A cell sorting system and a bad cell sorting method

Abstract

The present invention belongs to the field of cell sorting technology, and specifically relates to a cell sorting system and a method for sorting bad cells. The cell sorting system includes: at least one cell screening device, and a controller for controlling the cell screening device; the cell screening device includes: a culture dish, and a vibrator is arranged below the culture dish; wherein the vibrator is electrically connected to the controller; a water outlet is provided on the side wall of the culture dish, and a water outlet valve is arranged at the water outlet; the controller controls the vibrator to vibrate so that the non-adherent cells in the culture dish float up; after the liquid level of the culture fluid in the culture dish is higher than the water outlet, the controller controls the water outlet valve to open to discharge the culture fluid containing the non-adherent cells. The cell sorting system has a simple structure, can realize cell sorting, and improve the cell collection rate.

Description

Technical Field

[0001] The invention belongs to the technical field of cell biology, and in particular relates to a cell sorting system and a method for sorting bad cells. Background Art

[0002] Flow cytometers have a sorting function, but it relies on specific antibodies to label cells. When cells flow through a small tube, they are collected individually. However, cells require a 37-degree environment and will be damaged if they pass through the tube individually. Therefore, the survival rate of the cells collected has always been a problem, and the sorting machine costs more than one million.

[0003] Therefore, it is necessary to design a cell sorting system to improve the cell collection rate. Summary of the invention

[0004] The purpose of the present invention is to provide a cell sorting system and a bad cell sorting method.

[0005] In order to solve the above technical problems, the present invention provides a cell sorting system, comprising:

[0006] at least one cell screening device, and a controller for controlling the cell screening device;

[0007] The cell screening device comprises: a culture dish, a vibrator is arranged below the culture dish;

[0008] The vibrator is electrically connected to the controller;

[0009] The side wall of the culture dish is provided with a water outlet, and a water outlet valve is arranged at the water outlet;

[0010] The controller controls the vibrator to vibrate so that the non-adherent cells in the culture dish float up;

[0011] After the liquid level of the culture liquid in the culture dish is higher than the water outlet, the controller controls the water outlet valve to open to discharge the culture liquid containing the non-adherent cells.

[0012] Furthermore, the culture dish is also provided with a water inlet, and a water inlet valve is provided at the water inlet;

[0013] When the controller controls the water inlet valve to open, the culture fluid is added to the culture dish from the water inlet.

[0014] Furthermore, a liquid level meter is provided in the culture dish, and the liquid level meter is connected to the controller; and

[0015] The controller controls the opening of the water inlet valve and the water outlet valve through the liquid level meter, so that the liquid level of the culture liquid in the culture dish is kept approximately level with the height of the upper edge of the water outlet.

[0016] Furthermore, if there are more than two cell screening devices, a cascade setting is adopted, that is,

[0017] The water outlet of the previous cell screening device is connected to the water inlet of the next cell screening device, so that the non-adherent cells of the previous stage are transported to the next cell screening device for cultivation, thereby achieving cell sorting.

[0018] In another aspect, the present invention further provides a method for sorting bad cells, comprising:

[0019] Use cell sorting system to sort out bad cells.

[0020] Furthermore, the working method is suitable for implementing bad cell sorting using the cell sorting system.

[0021] Further, the controller controls the vibrator to vibrate so as to make the bad cells in the culture dish float up;

[0022] When the liquid level of the culture fluid in the culture dish is higher than the water outlet, the controller controls the water outlet valve to open to discharge the culture fluid containing bad cells so that the adherent cells are retained.

[0023] The beneficial effect of the present invention is that a cell sorting system and a bad cell sorting method of the present invention are provided with a vibrator under the culture dish, a water outlet is provided on the side wall of the culture dish, a water outlet valve is provided at the water outlet, and a controller controls the vibrator to vibrate so that the non-adherent cells in the culture dish float up; after the liquid level of the culture fluid in the culture dish is higher than the water outlet, the controller controls the water outlet valve to open to discharge the culture fluid containing the non-adherent cells. The cell sorting system has a simple structure, can sort and collect a large number of cells at one time, and improves the cell collection rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0025] Figure 1 is a schematic diagram of a cell sorting system of the present invention;

[0026] Figure 2 It is a schematic diagram of the serial arrangement of the cell sorting system of the present invention.

[0027] In the figure:

[0028] Vibrator 1, culture dish 2, water inlet 201, water outlet 202, water pump 3. DETAILED DESCRIPTION

[0029] The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic diagrams, which only illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0030] Example 1

[0031] Figure 1 is a schematic diagram of a cell sorting system of the present invention;

[0032] like Figure 1 As shown, this embodiment provides a cell sorting system, comprising: at least one cell screening device, and a controller for controlling the cell screening device; the cell screening device comprises: a culture dish 2, a vibrator 1 is arranged below the culture dish 2; wherein the vibrator 1 is electrically connected to the controller; a water outlet 202 is opened on the side wall of the culture dish 2, and a water outlet valve is arranged at the water outlet 202; the controller controls the vibrator 1 to vibrate so that the non-adherent cells in the culture dish 2 float up; after the liquid level of the culture fluid in the culture dish 2 is higher than the water outlet 202, the controller controls the water outlet valve to open to discharge the culture fluid containing the non-adherent cells.

[0033] In this embodiment, the start time, duration and frequency of vibration can be adjusted according to the type of cells. For example, vibration can be started 3 hours after the cells are cultured, the vibration duration can be 1 minute, and the vibration frequency can be in the range of 30-60HZ.

[0034] Furthermore, the culture dish 2 is also provided with a water inlet 201, and a water inlet valve is provided at the water inlet 201; when the controller controls the water inlet valve to open, the culture fluid is added to the culture dish 2 from the water inlet 201 to ensure that the culture fluid in the culture dish 2 can keep the cells growing and growing when there is no one on duty; thereby improving the degree of automation of the cell sorting system.

[0035] In this embodiment, a liquid level meter is provided in the culture dish 2, and the liquid level meter is connected to the controller; and the controller controls the opening of the water inlet valve and the water outlet valve through the liquid level meter, so that the liquid level of the culture liquid in the culture dish 2 is kept approximately level with the upper edge height of the water outlet 202, so that the suspended non-adherent cells can flow out of the water outlet 202 as much as possible, thereby improving the sorting efficiency.

[0036] The controller can adopt an industrial control board with an embedded processor and a plurality of IO ports, which can be connected to a vibrator, and the vibrator can be but not limited to a vibration motor; and the industrial control board also has a display interface and a USB interface, which can be used to connect a display and a keyboard to set corresponding parameters.

[0037] For example, when cardiomyocytes are collected, a small amount of fibroblasts will be mixed in. At this time, the fibroblasts need to be sorted out. The fibroblasts adhere to the wall faster than the cardiomyocytes. After the fibroblasts adhere to the wall, the controller controls the vibrator to vibrate so that the cardiomyocytes in the culture dish 2 float up. After the controller controls the water inlet valve to open, the culture fluid is added to the culture dish 2 from the water inlet 201. When the liquid level of the culture fluid in the culture dish 2 is higher than the water outlet 202, the controller controls the water outlet valve to open to discharge the culture fluid containing the cardiomyocytes, so that the fibroblasts are retained, and the discharged culture fluid containing the cardiomyocytes is collected for use.

[0038] Example 2

[0039] Figure 2 It is a schematic diagram of the serial arrangement of the cell sorting system of the present invention.

[0040] like Figure 2 As shown, based on Example 1, this embodiment provides a cell sorting system. If there are more than two cell screening devices, a serial setting is adopted, that is, the water outlet 202 of the cell screening device of the previous stage is connected to the water inlet 201 of the cell screening device of the next stage, so as to transport the non-adherent cells of the previous stage to the cell screening device of the next stage for cultivation, thereby realizing cell sorting.

[0041] Among them, the water outlet 202 of the previous level cell screening device is connected with the water inlet 201 of the next level cell screening device through a water pump 3, so that the low-position water outlet 202 of the culture dish 2 in the previous level cell screening device can smoothly transport the non-adherent cells to the high-position water inlet 201 of the culture dish 2 in the next level cell screening device.

[0042] Specifically, according to the different adhesion times of various cells, the controller controls the starting time of the vibrator 1 in the corresponding cell screening device; that is, after the corresponding cell screening device of the previous stage vibrates, the non-adherent cells in the culture dish 2 of this stage float up; after the liquid level of the culture fluid in the culture dish 2 of the current stage is higher than the water outlet 202, the controller controls the water outlet valve to open, so as to discharge the culture fluid containing non-adherent cells to the cell screening device of the next stage, so that the adherent cells are retained; sorting is carried out successively, so that the same type of cells are retained in the culture dish 2 in the corresponding cell screening device, thereby realizing cell sorting.

[0043] In this embodiment, the cell adhesion time of various cells is different according to different tissue acquisition methods. The types and concentrations of digestive enzymes (collagenase and trypsin, etc.) used when collecting cells will also cause differences in cell adhesion time. Therefore, the adhesion time of various cells in this embodiment can be specifically set according to the actual needs of cell cultivation. Those skilled in the art can set the specific start time and duration of vibration and the frequency of vibration according to the type of cells.

[0044] Example 3

[0045] Based on Example 1, this example provides a method for sorting bad cells, including: using the cell sorting system in Example 1 to sort bad cells.

[0046] The bad cells refer to cells in a bad state, which will produce lysed fragments. Due to gravity, the fragments will still stick to the bottom of the culture dish 2, so the vibrator is used to vibrate the fragments to float up and float on the upper layer.

[0047] The operation steps of this embodiment are as follows: the controller controls the vibrator to vibrate so that the bad cells in the culture dish 2 float up; when the liquid level of the culture fluid in the culture dish 2 is higher than the water outlet 202, the controller controls the water outlet valve to open to discharge the culture fluid containing the bad cells so that the adherent cells are retained.

[0048] In summary, a cell sorting system and a method for sorting bad cells of the present invention are provided with a vibrator 1 below a culture dish 2, a water outlet 202 is provided on the side wall of the culture dish 2, a water outlet valve is provided at the water outlet 202, and a controller controls the vibrator 1 to vibrate so that the non-adherent cells in the culture dish 2 float up; after the liquid level of the culture fluid in the culture dish 2 is higher than the water outlet 202, the controller controls the water outlet valve to open to discharge the culture fluid containing the non-adherent cells. The cell sorting system has a simple structure and can sort and collect a large number of cells at one time, thereby improving the cell collection rate; the cell sorting system can be provided with N-1 cell screening devices according to the sorting of N types of cells, and can sort multiple cells, which has the advantage of strong practicality in screening cells.

[0049] Based on the above ideal embodiments of the present invention, the relevant staff can make various changes and modifications without departing from the technical concept of the present invention through the above description. The technical scope of the present invention is not limited to the contents of the specification, and its technical scope must be determined according to the scope of the claims.

Claims

1. A method for sorting bad cells, comprising: A cell sorting system is used to sort out bad cells; A cell sorting system, comprising: at least one cell screening device, and a controller for controlling the cell screening device; the cell screening device comprises: a culture dish, a vibrator is arranged below the culture dish; wherein the vibrator is electrically connected to the controller; a water outlet is provided on the side wall of the culture dish, and a water outlet valve is arranged at the water outlet; the controller controls the vibrator to vibrate so that bad cells in the culture dish float up; after the liquid level of the culture fluid in the culture dish is higher than the water outlet, the controller controls the water outlet valve to open so as to discharge the culture fluid containing bad cells; The culture dish is also provided with a water inlet, and a water inlet valve is provided at the water inlet; when the controller controls the water inlet valve to open, the culture liquid is added to the culture dish from the water inlet; A liquid level meter is provided in the culture dish, and the liquid level meter is connected to the controller; and the controller controls the opening of the water inlet valve and the water outlet valve through the liquid level meter, so that the liquid level of the culture liquid in the culture dish is kept approximately level with the upper edge height of the water outlet; The vibration start time, duration and vibration frequency of the vibrator are adjusted according to the type of cells.

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