Dilution solution injection system and sample analyzer

Abstract

The present application discloses a diluent perfusion system and a sample analyzer, the diluent perfusion system comprises: a power source; a first liquid circuit and a second liquid circuit whose volume is larger than that of the first liquid circuit, the input ends of the first liquid circuit and the second liquid circuit are connected to the diluent barrel, the output ends of the first liquid circuit and the second liquid circuit are connected to the power source, and the node of the input ends of the first liquid circuit and the second liquid circuit is defined as the first node; a controller, which is electrically connected to the first liquid circuit, the second liquid circuit and the power source, and is used to close the first liquid circuit and conduct the second liquid circuit after controlling the first liquid circuit to be turned on and the power source to transport the diluent in the diluent barrel to the first node through the first liquid circuit, so that the diluent is transported to the diluent storage device arranged on the second liquid circuit through the second liquid circuit. Through the above-mentioned diluent perfusion system, the time of perfusing the diluent can be saved, and the purpose of rapid perfusion can be achieved.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and in particular to a diluent perfusion system and a sample analyzer. Background Art

[0002] In the field of medical devices, sample analyzers are common in vitro diagnostic analysis instruments, generally used for testing and analyzing samples such as blood samples or body fluids. Sample analyzers can include multiple test items. As one of the important reagents for providing sample reaction environment and sample analysis, diluent is an indispensable reagent for sample analysis. Generally speaking, a diluent storage device is provided inside the sample analyzer. When the sample analyzer is actually operating, it is necessary to draw the diluent from the diluent reagent bottle outside the sample analyzer into the diluent storage device inside the analyzer. Some manufacturers currently use an external gas source to establish negative pressure to fill the diluent storage device, which is a relatively expensive method; some manufacturers use a 10ml diluent syringe to fill the diluent storage device, and the filling efficiency is relatively low.

[0003] Therefore the prior art needs to be improved. Summary of the invention

[0004] In view of this, the present application provides a diluent perfusion system and a sample analyzer, which can save the time of perfusing the diluent and achieve the purpose of rapid perfusion.

[0005] In order to solve the above technical problems, a technical solution adopted in the present application is to provide a diluent perfusion system, comprising:

[0006] A power source, used to provide power;

[0007] A first liquid circuit and a second liquid circuit provided with a diluent storage device, wherein an input end of the first liquid circuit is connected to an input end of the second liquid circuit and then connected to a diluent barrel located outside the sample analyzer, and an output end of the first liquid circuit is connected to an output end of the second liquid circuit and then connected to the power source, wherein a node between the input end of the first liquid circuit and the input end of the second liquid circuit is defined as a first node;

[0008] A controller is electrically connected to the first liquid circuit, the second liquid circuit and the power source, and is used to control the first liquid circuit to be closed and the second liquid circuit to be connected after controlling the first liquid circuit to be connected and the power source to be turned on, when the power source transports the diluent in the diluent barrel to the first node through the first liquid circuit, so that the diluent is transported to the diluent storage device through the second liquid circuit under the action of the power source, wherein the volume of the first liquid circuit is smaller than the volume of the second liquid circuit.

[0009] In order to solve the above technical problem, another technical solution adopted by the present application is: to provide a sample analyzer, including the above diluent perfusion system.

[0010] The beneficial effect is that the present application sets a first liquid circuit with a smaller volume than the second liquid circuit. After the power source transports the diluent in the diluent barrel to the first node through the first liquid circuit, the second liquid circuit is used to transport the diluent to the diluent storage device in the second liquid circuit, instead of directly transporting the diluent in the diluent barrel to the first node through the second liquid circuit with a larger volume and then transporting it to the diluent storage device, thereby saving the time for transporting the diluent to the first node and achieving the purpose of rapid infusion as a whole. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. Among them:

[0012] Figure 1 It is a structural schematic diagram of an embodiment of the diluent perfusion system of the present application;

[0013] Figure 2 It is a structural schematic diagram of another embodiment of the diluent perfusion system of the present application;

[0014] Figure 3 It is a structural schematic diagram of an implementation method of a sample analyzer of the present application. DETAILED DESCRIPTION

[0015] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0016] See also Figure 1 , Figure 1 1 is a schematic structural diagram of an embodiment of a diluent injection system of the present application. The diluent injection system 1 comprises: a power source 10 , a first liquid path 20 , a second liquid path 30 and a controller 40 .

[0017] The diluent filling system 1 is located inside the sample analyzer and is used to fill the diluent outside the sample analyzer into the sample analyzer. The power source 10 is used to provide power, which can transport the diluent outside the sample analyzer into the sample analyzer.

[0018] The second liquid path 30 is provided with a diluent storage device 31 , and the diluent poured into the sample analyzer is stored in the diluent storage device 31 .

[0019] The input end 201 of the first liquid circuit 20 is connected to the input end 301 of the second liquid circuit 30 and then connected to the diluent barrel 2 located outside the sample analyzer. The diluent barrel 2 is filled with diluent. The function of the diluent perfusion system 1 is to perfuse the diluent in the diluent barrel 2 into the diluent storage device 31. The output end 202 of the first liquid circuit 20 is connected to the output end 302 of the second liquid circuit 30 and then connected to the power source 10.

[0020] Since the input end 201 of the first liquid circuit 20 is connected to the input end 301 of the second liquid circuit 30 and then connected to the diluent barrel 2 located outside the sample analyzer, there will be a node 21 between the input end 201 of the first liquid circuit 20 and the input end 301 of the second liquid circuit 30. In this embodiment, the node 21 is defined as the first node 21.

[0021] The controller 40 is electrically connected to the first fluid path 20 , the second fluid path 30 and the power source 10 , and is used to control the opening and closing of the first fluid path 20 , the second fluid path 30 and the opening and closing of the power source 10 .

[0022] Specifically, when it is necessary to use the diluent perfusion system 1 to perfuse the diluent storage device 31, the controller 40 controls the first liquid circuit 20 to be turned on and the power source 10 to be turned on. At this time, the power source 10 transports the diluent in the diluent barrel 2 through the first liquid circuit 20. When the power source 10 transports the diluent in the diluent barrel 2 to the first node 21 through the first liquid circuit 20, the first liquid circuit 20 is controlled to be closed and the second liquid circuit 30 is turned on. At this time, the power source 10 transports the diluent through the second liquid circuit 30, wherein the diluent previously transported to the first node 21 is transported to the diluent storage device 31 through the second liquid circuit 30, and at the same time, the diluent in the diluent barrel 2 continues to be transported to the diluent storage device 31 through the second liquid circuit 30 under the action of the power source 10.

[0023] In the present embodiment, the volume of the first liquid path 20 is smaller than the volume of the second liquid path 30 .

[0024] In the prior art, the diluent perfusion system 1 usually only has a second liquid circuit 30 provided with a diluent storage device 31. When the diluent needs to be perfused, the power source 10 directly perfuses the diluent outside the sample analyzer into the diluent storage device 31 through the second liquid circuit 30 via the input end 301 of the second liquid circuit 30.

[0025] If the time taken by the power source 10 to transport the diluent in the diluent barrel 2 to the first node 21 through the first liquid path 20 is recorded as t 1 , the time for the power source 10 to transport the diluent in the diluent barrel 2 to the first node 21 through the second liquid path 30 is recorded as t 2， Since the volume of the first liquid path 20 is smaller than that of the second liquid path 30, t 1 Less than t 2 That is to say, in this embodiment, since the first liquid path 20 is provided, and before the second liquid path 30 is used to perfuse the diluent, the first liquid path 20 with a smaller volume is used in advance to transport the diluent to the first node 21, that is, the input end 301 of the second liquid path 30. Compared with the prior art in which the diluent in the diluent barrel 2 is directly transported to the first node 21 through the second liquid path 30 with a larger volume and then transported to the diluent storage device 31, the time for transporting the diluent to the input end 301 of the second liquid path 30 can be saved, thereby achieving the purpose of quickly perfusing the diluent as a whole.

[0026] In one application scenario, the controller 40 is specifically used to: when the time for controlling the conduction of the first liquid circuit 20 reaches a preset time threshold, control the first liquid circuit 20 to close and the second liquid circuit 30 to conduct. Specifically, when the conduction time of the first liquid circuit 20 reaches a preset time threshold, the diluent in the diluent barrel 2 is just transported to the first node 31, wherein the time threshold can be obtained by a technician in the field through multiple experiments or calculations, and is not limited here. Of course, in other embodiments, a liquid level detection sensor electrically connected to the controller 40 can also be set at the first node 21. When the liquid level detection sensor detects that the liquid level at the first node 21 reaches the requirement, the controller 40 controls the first liquid circuit 20 to close and controls the second liquid circuit 30 to conduct.

[0027] See also Figure 2 , Figure 2 It is a structural schematic diagram of another embodiment of the diluent perfusion system of the present application. In this embodiment, the first liquid circuit 20 specifically includes: a first two-way solenoid valve 22, and the second liquid circuit 30 specifically includes: a second two-way solenoid valve 32 and a third two-way solenoid valve 33.

[0028] The first two-way solenoid valve 22 is electrically connected to the controller 40, and the two ends of the first two-way solenoid valve 22 are respectively connected to the input end 301 of the second liquid circuit 30 and the output end 302 of the second liquid circuit 30. The controller 40 controls the conduction and closing of the first liquid circuit 20 through the first two-way solenoid valve 22. Specifically, when the controller 40 controls the first two-way solenoid valve 22 to open, the first liquid circuit 20 is connected, and when the controller 40 controls the first two-way solenoid valve 22 to close, the first liquid circuit 20 is closed.

[0029] The second two-way solenoid valve 32, the diluent storage device 31 and the third two-way solenoid valve 33 are connected in sequence, the end of the second two-way solenoid valve 32 not connected to the diluent storage device 31 is connected to the input end 201 of the first liquid circuit 20, the end of the third two-way solenoid valve 33 not connected to the diluent storage device 31 is connected to the output end 202 of the first liquid circuit 20, the second two-way solenoid valve 32 and the third two-way solenoid valve 33 are electrically connected to the controller 40, respectively, and the controller 40 controls the opening and closing of the second liquid circuit 30 through the second two-way solenoid valve 32 and the third two-way solenoid valve 33. Specifically, when the controller 40 controls the second two-way solenoid valve 32 and the third two-way solenoid valve 33 to be opened, the second liquid circuit 30 is opened, and when the controller 40 controls the second two-way solenoid valve 32 and the third two-way solenoid valve 33 to be closed, the second liquid circuit 30 is closed.

[0030] In this embodiment, the power source 10 is a liquid pump, which is used to form a negative pressure in the first liquid path 20 / the second liquid path 30 when the first liquid path 20 / the second liquid path 30 are connected. Specifically, when the power source 10 is working, the power source 10 draws out the air in the connected first liquid path 20 / the second liquid path 30 to form a negative pressure, so that the diluent in the diluent barrel 2 can be transported through the first liquid path 20 / the second liquid path 30.

[0031] When the second liquid path 30 is connected, in order to prevent the liquid pump from generating too much suction force in the second liquid path 30, thereby causing the diluent entering the diluent storage device 31 to generate a large number of small bubbles, thereby affecting the accuracy of the detection result, such as Figure 2 As shown, the second liquid circuit 30 also includes: a buffer tank 34, which is located between the diluent storage device 31 and the third two-way solenoid valve 33, and is used to balance the pressure in the second liquid circuit 30 during perfusion, relieve the suction force formed by the liquid pump in the second liquid circuit 30, and avoid the generation of small bubbles in the diluent.

[0032] Continue reading Figure 2 In order to achieve the connection between the first liquid circuit 20 and the second liquid circuit 30, the diluent perfusion system 1 also includes: a first three-way joint 51 and a second three-way joint 52. The input end 201 of the first liquid circuit 20 and the input end 301 of the second liquid circuit 30 are connected to the diluent barrel 2 through the first three-way joint 51, and the output end 202 of the first liquid circuit 20 and the output end 302 of the second liquid circuit 30 are connected to the power source 10 through the second three-way joint 52.

[0033] That is, the node 21 between the input end 201 of the first liquid circuit 20 and the input end 301 of the second liquid circuit 30 is the first three-way joint 51. When the controller 40 transports the diluent in the diluent barrel 2 to the first three-way joint 51 through the first liquid circuit 20, the controller 40 controls the first liquid circuit 20 to be closed and the second liquid circuit 30 to be connected.

[0034] At the same time, in order to prevent failure during the perfusion process, for example, when the diluent has reached the first node 21, the controller 40 has not yet controlled the first liquid path 20 to be closed, and the diluent will be transported to the first liquid path 20, or when the diluent storage device 31 is too full of diluent and the liquid pump continues to perfuse the diluent into the diluent storage device 31 through the second liquid path 30, the power source 10 in this embodiment is also connected to the waste liquid barrel 3 outside the sample analyzer to ensure that excess diluent in the entire diluent perfusion system 1 is discharged into the waste liquid barrel 3 outside the sample analyzer.

[0035] Continue reading Figure 2 The diluent injection system 1 further includes: a diluent injector 61 , a first three-way solenoid valve 62 , an air filter 71 and a second three-way solenoid valve 72 .

[0036] The first three-way solenoid valve 62 is electrically connected to the controller 40, the common end 621 of the first three-way solenoid valve 62 is connected to the diluent syringe 61, the normally closed end 622 of the first three-way solenoid valve 62 is connected to the diluent storage device 31, and the normally open end 623 of the first three-way solenoid valve 62 is connected to the reaction pool 4 of the sample analyzer. When the diluent syringe 61 needs to absorb the diluent in the diluent storage device 31, the controller 40 controls the first three-way solenoid valve 62 to be powered on, the diluent syringe 61 is connected to the diluent storage device 31, and the diluent syringe 61 is driven by a motor (not shown) to absorb a certain amount of diluent from the diluent storage device 31. When the diluent syringe 61 needs to deliver the absorbed diluent to the reaction pool 4, the controller 40 controls the first three-way solenoid valve 62 to be powered off, at which time the diluent syringe 61 is connected to the reaction pool 4, and the diluent syringe 61 injects the absorbed diluent into the reaction pool 4 driven by the motor.

[0037] At the same time, in order to maintain the liquid level balance in the diluent storage device 31, when the diluent syringe 61 needs to absorb the diluent in the diluent storage device 31, the controller 40 controls the first three-way solenoid valve 62 to be powered on, and also controls the second two-way solenoid valve 32 to be opened, so that the diluent in the diluent barrel 2 is poured into the diluent storage device 31 through the second liquid path 30.

[0038] The second three-way solenoid valve 72 is electrically connected to the controller 40, the common end 721 of the second three-way solenoid valve 72 is connected to the diluent storage device 31, the normally open end 723 of the second three-way solenoid valve 72 is connected to the buffer tank 34, and the normally closed end 722 of the second three-way solenoid valve 72 is connected to the air filter 71. It can be understood that in other embodiments, if there is no buffer tank 34, the normally open end 723 of the second three-way solenoid valve 72 is connected to the third two-way solenoid valve 33.

[0039] After the diluent filling system 1 has filled the diluent, when the diluent syringe 61 absorbs the diluent, the inside of the diluent syringe 61 is in a negative pressure state. Since in the previous filling process, under the action of the power source 10, the diluent storage device 31 may also be in a negative pressure state. In order to avoid excessive negative pressure in the entire diluent filling system 1 and cause damage to the device, the air filter 71 needs to periodically connect the diluent storage device 31 to the atmosphere after the diluent syringe 61 absorbs the diluent in the diluent storage device 31 to maintain the pressure balance in the diluent storage device 31. When the air filter 71 needs to connect the diluent storage device 31 to the atmosphere, the controller 40 controls the second three-way solenoid valve 72 to power on, and the air filter 71 is connected to the second three-way solenoid valve 72. When the air filter 71 needs to stop connecting the diluent storage device 31 to the atmosphere, the controller 40 controls the second three-way solenoid valve 72 to power off, and the second three-way solenoid valve 72 is connected to the buffer tank 34.

[0040] Continue reading Figure 2 The diluent filling system 1 further includes: a float sensor 80 , a heater 91 and a temperature sensor 92 .

[0041] The float sensor 80 is electrically connected to the controller 40 and is disposed in the diluent storage device 31 for monitoring the liquid level in the diluent storage device 31 so that when the liquid level in the diluent storage device 31 reaches a preset requirement, the controller 40 controls the second liquid path 30 to be closed.

[0042] The heater 91 and the temperature sensor 92 are located in the diluent storage device 31, and both are electrically connected to the controller 40. When the temperature sensor 92 measures that the temperature of the diluent in the diluent storage device 31 is lower than the target temperature, the controller 40 controls the heater 91 to heat the diluent in the diluent storage device 31 until the temperature of the diluent reaches the target temperature, wherein the heater 91 can be a heating plate or a heating coil.

[0043] Combine the following Figure 2 , the working method of the diluent perfusion system 1 is introduced in detail.

[0044] When it is necessary to use the diluent filling system 1 to fill the diluent, the controller 40 controls the first two-way solenoid valve 22 to open and controls the power source 10 to open. At this time, the first liquid circuit 20 is connected. Under the action of the power source 10, the diluent in the diluent barrel 2 is transported from the diluent barrel 2. When the diluent is transported to the first three-way joint 51, that is, the first node 21, the controller 40 controls the first two-way solenoid valve 22 to close, and controls the second two-way solenoid valve 32 and the third two-way solenoid valve 33 to open. At this time, the second liquid circuit 30 is connected, and the diluent previously transported to the first three-way joint 51 and the diluent in the diluent barrel 2 are transported to the diluent storage device 31 through the second liquid circuit 30. When the float sensor 80 detects that the liquid level in the diluent storage device 31 reaches the preset requirement, the controller 40 controls the second two-way solenoid valve 32 and the third two-way solenoid valve 33 to close. When the diluent injector 61 needs to absorb the diluent, the controller 40 controls the first three-way solenoid valve 62 to be powered on, and controls the second two-way solenoid valve 32 to be opened, and the diluent injector 61 absorbs the diluent under the action of the motor. After the absorption is completed, the controller 40 controls the second two-way solenoid valve 32 to be closed and controls the first three-way solenoid valve 62 to be powered off. The diluent injector 61 injects the absorbed diluent into the reaction pool 4 under the action of the motor. At this time, if it is necessary to connect the diluent storage device 31 to the atmosphere to balance its pressure, the controller 40 controls the second three-way solenoid valve 72 to be powered on, and injects the atmosphere into the diluent storage device 31 through the air filter 71. When the pressure reaches the requirement, the controller 40 controls the second two-way solenoid valve 72 to be powered off. In the operation process of the entire diluent perfusion system 1, if the temperature sensor 92 detects that the temperature of the diluent in the diluent storage device 31 is lower than the target temperature, the controller 40 controls the heater 91 to work until the temperature of the diluent reaches the target temperature.

[0045] See also Figure 3 , Figure 3 1 is a schematic diagram of the structure of an embodiment of a sample analyzer of the present application. The sample analyzer 5 includes the diluent perfusion system 1 in any of the above embodiments. The detailed structure can be found in the above embodiments and will not be described again.

[0046] The sample analyzer 5 may be a biological detection device such as a flow cytometer, a blood cell analyzer, etc., which is not limited here.

[0047] The above description is only an implementation method of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A diluent perfusion system, characterized in that: include: A power source, used to provide power; A first liquid circuit and a second liquid circuit provided with a diluent storage device, wherein an input end of the first liquid circuit is connected to an input end of the second liquid circuit and then connected to a diluent barrel located outside the sample analyzer, and an output end of the first liquid circuit is connected to an output end of the second liquid circuit and then connected to the power source, wherein a node between the input end of the first liquid circuit and the input end of the second liquid circuit is defined as a first node; a controller, electrically connected to the first liquid circuit, the second liquid circuit and the power source, for controlling the first liquid circuit to be closed and the second liquid circuit to be connected after controlling the first liquid circuit to be connected and the power source to be turned on, when the power source transports the diluent in the diluent barrel to the first node through the first liquid circuit, so that the diluent is transported to the diluent storage device through the second liquid circuit under the action of the power source, wherein the volume of the first liquid circuit is smaller than the volume of the second liquid circuit; Wherein, the diluent perfusion system further comprises a liquid level detection sensor, and when the liquid level detection sensor detects that the liquid level at the first node reaches the requirement, the controller controls the first liquid path to be closed and controls the second liquid path to be opened; And / or, the controller is specifically used to: when the time for controlling the conduction of the first liquid circuit reaches a preset time threshold, control the first liquid circuit to be closed and the second liquid circuit to be connected; Wherein, the first liquid circuit comprises: a first two-way solenoid valve, which is electrically connected to the controller, and two ends of the first two-way solenoid valve are respectively connected to an input end of the second liquid circuit and an output end of the second liquid circuit, and the controller controls the opening and closing of the first liquid circuit through the first two-way solenoid valve; Wherein, the diluent perfusion system also includes: a first three-way joint and a second three-way joint, the first liquid circuit input end and the second liquid circuit input end are connected to the diluent barrel through the first three-way joint, the first liquid circuit output end and the second liquid circuit output end are connected to the power source through the second three-way joint, and the power source is also connected to the waste liquid barrel outside the sample analyzer.

2. The diluent perfusion system according to claim 1, characterized in that: The second liquid circuit includes: a second two-way solenoid valve and a third two-way solenoid valve, the second two-way solenoid valve, the diluent storage device and the third two-way solenoid valve are connected in sequence, the end of the second two-way solenoid valve not connected to the diluent storage device is connected to the input end of the first liquid circuit, the end of the third two-way solenoid valve not connected to the diluent storage device is connected to the output end of the first liquid circuit, the second two-way solenoid valve and the third two-way solenoid valve are electrically connected to the controller respectively, and the controller controls the conduction and closing of the second liquid circuit through the second two-way solenoid valve and the third two-way solenoid valve.

3. The diluent perfusion system according to claim 2, characterized in that: The power source is a liquid pump, which is used to form a negative pressure in the first liquid path or the second liquid path when the first liquid path or the second liquid path is connected; The second liquid circuit further includes: a buffer tank, located between the diluent storage device and the third two-way solenoid valve, for buffering the suction force of the liquid pump.

4. The diluent perfusion system according to claim 3, characterized in that: The diluent perfusion system also includes: a diluent syringe and a first three-way solenoid valve, wherein the first three-way solenoid valve is electrically connected to the controller, a normally closed end of the first three-way solenoid valve is connected to the diluent storage device, a normally open end is connected to the reaction pool of the sample analyzer, and a common end is connected to the diluent syringe. When the diluent syringe needs to absorb the diluent in the diluent storage device, the controller controls the first three-way solenoid valve to be powered on, and when the diluent syringe needs to deliver the absorbed diluent into the reaction pool, the controller controls the first three-way solenoid valve to be powered off.

5. The diluent perfusion system according to claim 4, characterized in that: The diluent filling system also includes: an air filter and a second three-way solenoid valve, wherein the second three-way solenoid valve is electrically connected to the controller, the common end of the second three-way solenoid valve is connected to the diluent storage device, the normally open end is connected to the buffer tank, and the normally closed end is connected to the air filter. When the air filter needs to connect the diluent storage device to the atmosphere to maintain the pressure balance in the diluent storage device after the diluent syringe absorbs the diluent in the diluent storage device, the controller controls the second three-way solenoid valve to be powered on, and when the air filter needs to stop connecting the diluent storage device to the atmosphere, the controller controls the second three-way solenoid valve to be powered off.

6. The diluent perfusion system according to claim 1, characterized in that: The diluent filling system further includes: a float sensor, which is electrically connected to the controller and disposed in the diluent storage device, and is used to monitor the liquid level in the diluent storage device, so that when the liquid level in the diluent storage device reaches a preset requirement, the controller controls the second liquid circuit to be closed.

7. A sample analyzer, characterized in that: Comprising the diluent perfusion system as described in any one of claims 1 to 6.

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