Method and culture medium for efficiently culturing dendrobium huoshanense seedlings

Abstract

The invention relates to a method for efficiently culturing dendrobium huoshanense seedlings. N6 is used as a base culture medium, and types of hormones, types of organic additives and mixing use proportions are determined at different growth stages. The method comprises steps of sterile sowing of dendrobium huoshanense, protocorm differentiation, seedling strengthening and rooting culture and test-tube plantlet transplantation. The adopted culture medium is unique and materials are available and not affected by production places and seasons, the method is high in operability, the germinationrate and differentiation rate of seeds are high, a large quantity of tissue cultured seedlings which are strong and more consistent in growth and have the transplantation survival rate larger than orequal to 95% are successfully obtained, and a new way is explored for artificial rapid propagation of dendrobium huoshanense.

Description

technical field

[0001] The invention relates to a seedling propagation method of rare and endangered medicinal plants, in particular to a method for efficiently cultivating seedlings of Dendrobium officinalis by mixing and using different organic additives. Background technique

[0002] Dendrobium huoshanense has high medicinal value and has been considered as the best product in Dendrobium since ancient times. Due to its low reproductive capacity and artificial over-harvesting, the wild resources are basically extinct, and it has become an endangered and rare plant. The "critically endangered" (first-level national protection) species in the "Red Catalog of Species in China".

[0003] Dendrobium is rich in polysaccharides, amino acids, dendrobine, dendrobine and more than ten kinds of alkaloids. According to the identification of the Institute of Medicinal Plants, Chinese Academy of Medical Sciences: the polysaccharide content of Dendrobium officinalis is 18.974%, and the ...

Examples

Embodiment 1

[0025] Embodiment 1: a kind of method of high-efficiency cultivation rice dendrobium seedling, comprises the following steps:

[0026] (1) Sterile sowing: take the mature and uncracked rice dendrobium capsules as explants, disinfect with 72% (V / V) alcohol for 50 seconds, rinse twice with sterile water, and then use 1% Disinfect with sodium hypochlorite for 10 minutes, then rinse with sterile water for 5 times and then dry; cut the fruit, use an inoculation needle to inoculate the yellow powdery seeds into a culture bottle containing about 100ml of seed germination medium for cultivation, and the seeds will germinate into protocorms , the seed germination medium is 1 / 2N6 medium+6-BA 1.0mg / L+NAA0.3mg / L+150g / L banana+25g / L sucrose+5g / L agar+0.8g / L activated carbon, The rest is water; a plump dark green and dense protocorm with a diameter of about 1.5-2.0 mm is obtained.

[0027] (2) Protocorm differentiation: Inoculate dense protocorms obtained by aseptic sowing into a culture b...

Embodiment 2

[0032] Embodiment 2 A method for efficiently cultivating rice seedlings of Dendrobium, comprising the following steps:

[0033] (1) Sterile sowing: take the mature and uncracked rice dendrobium capsules as explants, disinfect with 72% (V / V) alcohol for 50 seconds, rinse twice with sterile water, and then use 1% Disinfect with sodium hypochlorite for 10 minutes, then rinse with sterile water for 5 times and then dry; cut the fruit, use an inoculation needle to inoculate the yellow powdery seeds into a culture bottle containing about 100ml of seed germination medium for cultivation, and the seeds will germinate into protocorms , the seed germination medium is 1 / 2N6 medium+6-BA 1.0mg / L+NAA0.3mg / L+150g / L banana+25g / L sucrose+5g / L agar+0.8g / L activated carbon, The rest is water; a plump dark green and dense protocorm with a diameter of about 1.5-2.0 mm is obtained.

[0034] (2) Protocorm differentiation: Inoculate dense protocorms obtained by aseptic sowing into a culture bottle f...

Embodiment 3

[0038] Embodiment 3: a kind of method for efficiently cultivating rice seedlings of Dendrobium, comprises the following steps:

[0039] (1) Sterile sowing: take the mature and uncracked rice dendrobium capsules as explants, disinfect with 72% (V / V) alcohol for 50 seconds, rinse twice with sterile water, and then use 1% Disinfect with sodium hypochlorite for 10 minutes, then rinse with sterile water for 5 times and then dry; cut the fruit, use an inoculation needle to inoculate the yellow powdery seeds into a culture bottle containing about 100ml of seed germination medium for cultivation, and the seeds will germinate into protocorms , the seed germination medium is 1 / 2N6 medium+6-BA 1.0mg / L+NAA0.3mg / L+150g / L banana+25g / L sucrose+5g / L agar+0.8g / L activated carbon, The rest is water; a plump dark green and dense protocorm with a diameter of about 1.5-2.0 mm is obtained.

[0040] (2) Protocorm differentiation: Inoculate dense protocorms obtained by aseptic sowing into a culture ...

technical field

[0001] The invention relates to a seedling propagation method of rare and endangered medicinal plants, in particular to a method for efficiently cultivating seedlings of Dendrobium officinalis by mixing and using different organic additives. Background technique

[0002] Dendrobium huoshanense has high medicinal value and has been considered as the best product in Dendrobium since ancient times. Due to its low reproductive capacity and artificial over-harvesting, the wild resources are basically extinct, and it has become an endangered and rare plant. The "critically endangered" (first-level national protection) species in the "Red Catalog of Species in China".

[0003] Dendrobium is rich in polysaccharides, amino acids, dendrobine, dendrobine and more than ten kinds of alkaloids. According to the identification of the Institute of Medicinal Plants, Chinese Academy of Medical Sciences: the polysaccharide content of Dendrobium officinalis is 18.974%, and the ...

Examples

Embodiment 1

[0025] Embodiment 1: a kind of method of high-efficiency cultivation rice dendrobium seedling, comprises the following steps:

[0026] (1) Sterile sowing: take the mature and uncracked rice dendrobium capsules as explants, disinfect with 72% (V / V) alcohol for 50 seconds, rinse twice with sterile water, and then use 1% Disinfect with sodium hypochlorite for 10 minutes, then rinse with sterile water for 5 times and then dry; cut the fruit, use an inoculation needle to inoculate the yellow powdery seeds into a culture bottle containing about 100ml of seed germination medium for cultivation, and the seeds will germinate into protocorms , the seed germination medium is 1 / 2N6 medium+6-BA 1.0mg / L+NAA0.3mg / L+150g / L banana+25g / L sucrose+5g / L agar+0.8g / L activated carbon, The rest is water; a plump dark green and dense protocorm with a diameter of about 1.5-2.0 mm is obtained.

[0027] (2) Protocorm differentiation: Inoculate dense protocorms obtained by aseptic sowing into a culture b...

Embodiment 2

[0032] Embodiment 2 A method for efficiently cultivating rice seedlings of Dendrobium, comprising the following steps:

[0033] (1) Sterile sowing: take the mature and uncracked rice dendrobium capsules as explants, disinfect with 72% (V / V) alcohol for 50 seconds, rinse twice with sterile water, and then use 1% Disinfect with sodium hypochlorite for 10 minutes, then rinse with sterile water for 5 times and then dry; cut the fruit, use an inoculation needle to inoculate the yellow powdery seeds into a culture bottle containing about 100ml of seed germination medium for cultivation, and the seeds will germinate into protocorms , the seed germination medium is 1 / 2N6 medium+6-BA 1.0mg / L+NAA0.3mg / L+150g / L banana+25g / L sucrose+5g / L agar+0.8g / L activated carbon, The rest is water; a plump dark green and dense protocorm with a diameter of about 1.5-2.0 mm is obtained.

[0034] (2) Protocorm differentiation: Inoculate dense protocorms obtained by aseptic sowing into a culture bottle f...

Embodiment 3

[0038] Embodiment 3: a kind of method for efficiently cultivating rice seedlings of Dendrobium, comprises the following steps:

[0039] (1) Sterile sowing: take the mature and uncracked rice dendrobium capsules as explants, disinfect with 72% (V / V) alcohol for 50 seconds, rinse twice with sterile water, and then use 1% Disinfect with sodium hypochlorite for 10 minutes, then rinse with sterile water for 5 times and then dry; cut the fruit, use an inoculation needle to inoculate the yellow powdery seeds into a culture bottle containing about 100ml of seed germination medium for cultivation, and the seeds will germinate into protocorms , the seed germination medium is 1 / 2N6 medium+6-BA 1.0mg / L+NAA0.3mg / L+150g / L banana+25g / L sucrose+5g / L agar+0.8g / L activated carbon, The rest is water; a plump dark green and dense protocorm with a diameter of about 1.5-2.0 mm is obtained.

[0040] (2) Protocorm differentiation: Inoculate dense protocorms obtained by aseptic sowing into a culture ...