A method for maintaining stable population of Bactrocera dorsalis indoors

Abstract

The invention discloses a method for stably maintaining a population of orange fruit flies indoors. The orange fruit flies are raised in an insect cage of 40 cm×37 cm×40 cm. A rope is horizontally arranged above the cage, and a hanging hook made of iron wire is hung on the rope. An apple with a hole is fixed on the hanging hook. A white plastic box is placed at the bottom of the insect cage. The box contains sterilized sand. A mineral water bottle filled with water and with an absorbent cotton strip is placed near an operating port of the insect cage for the orange fruit flies to take water. The method for raising orange fruit flies has a simple and convenient operation process, and the population quantity is easy to maintain stably. The device required by the method is simple to assemble, has low cost, and can be recycled. The method of the invention can achieve stable maintenance of the orange fruit fly population even if the population quantity of the orange fruit fly is small (60 pairs) indoors. Less time is spent in the process of maintaining the population indoors. The method is an efficient method for maintaining the population.

Description

Technical Field

[0001] The invention relates to a method for stably maintaining a population of oriental fruit fly indoors, and belongs to the field of agricultural science and technology. Background Art

[0002] Bactrocera dorsalis Hendel belongs to the genus Bactrocera of the family Tephritidae in the order Diptera. It is also known as the Oriental fruit fly, commonly known as citrus maggots, fruit maggots, etc. This insect can harm a variety of fruit trees (citrus, mango, peach, pomegranate, jujube, pear, apple, etc.) and vegetables (eggplant, pepper, cucumber, tomato, etc.), and is a Class II quarantine pest in my country. The female adult of the Bactrocera dorsalis Hendel can lay eggs under the epidermis of fresh damaged fruits, and the eggs hatch into larvae in the fruit. As the larvae develop, their appetite gradually increases, and they expand to the deep layer of the fruit flesh to feed. After the fruits are damaged, they shrink and rot, which seriously affects the yield and even leads to a total crop failure.

[0003] Most of the orange fruit flies overwinter as pupae in the field, and their population will decrease rapidly in winter, which brings inconvenience to the development of orange fruit fly research. Therefore, the study of orange fruit flies in the laboratory is particularly important. During the experiment (drug efficacy and drug resistance mechanism, cold resistance, biological control, etc.), not only is there a great demand for this insect, but it also takes a long time to acclimate after being obtained in the field. In order to ensure the supply of various laboratory research, it is inevitable to raise multiple generations indoors. At this time, it is very important to keep the population stable indoors.

[0004] At present, the indoor breeding of the orange fruit fly is to raise each insect stage separately. After the insect lays eggs, the eggs are swept into the larval feed. After the larvae mature, they are allowed to pupate in the soil. Before the initial emergence, the pupae are screened out and placed in a new cage. After the emergence into adults, water is added, the feed is changed, and the eggs are swept every day. The whole breeding process is very cumbersome and time-consuming, and the materials used are relatively expensive. Improper operation has a great impact on the population of the orange fruit fly. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a method for stably maintaining the population of oriental fruit fly indoors in view of the deficiencies in the prior art. The breeding process is simple and convenient, and the population quantity is easy to maintain stable.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] A method for stably maintaining a population of Bactrocera dorsalis indoors comprises the following steps:

[0008] (1) Making insect cages:

[0009] A rectangular frame is constructed by using 12 plastic sticks and 8 adapters, and a rectangular mesh cover is constructed by using a gauze net, wherein the length, width and height of the mesh cover are slightly larger than the length, width and height of the frame; one of the four sides of the mesh cover can be opened, and the side is a delivery side, and the delivery side is connected to the top surface, the bottom surface and the two side surfaces of the mesh cover through a zipper A; the zipper A is unzipped, the frame is placed in the mesh cover to form an insect cage, and then a horizontally arranged rope is placed on the top surface of the frame, and the two ends of the rope are tied to the two long sides or the two short sides of the top surface of the frame to form a connecting rope; the zipper A is pulled up, and a semicircular delivery opening is opened on the delivery side, and the delivery opening is equipped with a semicircular shielding net, and the shielding net is connected to the delivery opening through a zipper B; a circular operating opening is opened on the shielding net, and the circular operating opening is connected to a cylindrical mesh sleeve, and the end of the mesh sleeve is open;

[0010] (2) Preparation of breeding place:

[0011] Put sterilized sand in a white plastic box, spread it evenly, and spray double distilled water on the sterilized sand to increase its humidity; then pour double distilled water into a mineral water bottle until it is full, and insert a sterilized absorbent cotton strip into the mineral water bottle to block the bottle mouth; then put the mineral water bottle with the blocked bottle mouth into the white plastic box with sterilized sand; finally, open zipper B, and put the white plastic box with the mineral water bottle and sterilized sand into the insect cage through the insertion port;

[0012] (3) Production and incubation site:

[0013] Take a clean apple, spray anhydrous ethanol evenly on the surface of the apple and dry it under sterile conditions; use sterilized tweezers to poke holes on the surface of the apple; fix the apple on a hook made of iron wire, and the hook goes through the stem pit to the calyx pit of the apple; then hang the hook on the connecting rope in the insect cage; finally, pull up the zipper B and tighten the operation port;

[0014] (4) Rearing and daily management of Bactrocera dorsalis:

[0015] Open zipper B, take 60 pairs (60 males and 60 females) of newly emerged orange fruit flies from the population raised in the laboratory, place them in an insect cage containing sterilized sand, mineral water bottles, and apples, and quickly zip up the cage to prevent the orange fruit flies from flying out; observe the water level of double distilled water in the mineral water bottle every 6 days to ensure that the water in the bottle is sufficient for the orange fruit flies; replace apples every 3 days before laying eggs, dissect the replaced apples, and observe whether there are eggs and larvae in the fruit; if no eggs and larvae are found, store the apples at -20℃ for 48 hours and then throw them away. If eggs and larvae are found, place the apples in a new insect cage; 8 days after the mature larvae in the new insect cage pupate, hang a punctured apple again for the newly emerged orange fruit flies to eat.

[0016] In the above technical solution, in step (1), the 12 plastic sticks are 8 40 cm long and 4 37 cm long plastic sticks, and the specifications of the skeleton are: length 40 cm × width 37 cm × height 40 cm, and the volume is 59200 cm 3 .

[0017] In the above technical solution, in step (1), the mesh cover has the specifications of 42 cm in length × 39 cm in width × 42 cm in height, and the mesh of the gauze is 200 meshes. The mesh cover needs to be slightly larger than the frame and should not cause damage to the gauze.

[0018] In the above technical solution, in step (1), the connecting rope is 43 cm long and should be tied tightly to prevent the apples from falling.

[0019] In the above technical solution, in step (1), the operation port has a diameter of 12 cm; and the cylindrical mesh sleeve has a sleeve length of 18 cm.

[0020] In the above technical solution, in step (2), the sterilized sand is sieved with a 20-mesh sieve and then placed in a white plastic box with a thickness of 4-5 cm; double distilled water is sprayed into the sterilized sand to increase its humidity to 65-75%.

[0021] In the above technical solution, in step (2), the sterilized absorbent cotton strip has a length of 28-32 cm, the top of which is 2-3 cm above the bottle mouth and the bottle mouth is completely blocked with absorbent cotton to prevent the citrus fruit fly from entering the bottle and being drowned.

[0022] In the above technical solution, in step (2), the mineral water bottle has a capacity of 550 mL, and the bottom of the bottle is covered with sterilized soil. The position of the mineral water bottle is close to the inlet of the insect cage to facilitate later operations.

[0023] In the above technical solution, in step (2), the white plastic box has the specifications of 37 cm long × 30 cm wide × 15 cm high, and must match the insect cage to prevent the insect cage from being damaged.

[0024] In the above technical solution, in step (3), the apple has a diameter of 8-12 cm, preferably the Red Fuji variety; ST-14 tweezers from VETUS Company are used to pierce the surface of the apple; and the length of the iron wire used to make the hanging hook is 18 cm.

[0025] In the above technical solution, in step (4), the double distilled water in the mineral water bottle shall not be less than 2 / 3 (if it is a 550 ml bottle, the double distilled water shall not be less than 380 ml) to prevent the absorbent cotton on the bottle mouth from drying out.

[0026] In the above technical solution, the double distilled water is a kind of double distilled water, which is water that has been distilled twice, and prevents the citrus fruit fly from dying due to unclean water.

[0027] The present invention also provides a breeding cage for stably maintaining the population of Bactrocera dorsalis indoors, wherein the main body is a rectangular parallelepiped frame, and the outside of the frame is covered with a rectangular parallelepiped mesh cover, and the length, width and height of the mesh cover are slightly larger than the length, width and height of the frame, and the characteristics are:

[0028] The frame has a connecting rope disposed transversely on the top surface, and the two ends of the connecting rope are tied to the two long sides or the two short sides of the top surface of the frame;

[0029] The mesh cover has one side among the four sides that can be opened for delivery, and the delivery side is connected to the top surface, the bottom surface and the two side surfaces of the mesh cover through a zipper A;

[0030] A semicircular delivery opening is provided on the delivery side, and a semicircular shielding net is provided at the delivery opening, and the shielding net is connected to the delivery opening via a zipper B;

[0031] The shielding net is provided with a circular operation opening, and the circular operation opening is connected to a cylindrical net sleeve, the end of the net sleeve is open and can be tied with a knot or a rope;

[0032] A white plastic box is placed inside the mesh cover, and the white plastic box is also located inside the frame;

[0033] The white plastic box has sterilized sand in the bottom, and a mineral water bottle filled with double distilled water is placed inside, with the sterilized soil covering the bottom of the bottle;

[0034] The mouth of the mineral water bottle is blocked with a sterilized absorbent cotton strip;

[0035] The breeding cage is also provided with a hanging hook, one end of which is used for hanging apples, and the other end of which is hung on the connecting rope.

[0036] In the above technical solution, the skeleton has the following specifications: length 40cm×width 37cm×height 40cm, and the volume is 59200cm 3 .

[0037] In the above technical solution, the mesh cover has the specifications of 42cm in length×39cm in width×42cm in height, and the mesh size is 200 meshes.

[0038] In the above technical solution, the length of the connecting rope is 43 cm.

[0039] In the above technical solution, the blocking net has a mesh size of 200 meshes.

[0040] In the above technical solution, the operation port has a diameter of 12 cm.

[0041] In the above technical solution, the cylindrical mesh sleeve has a sleeve length of 18 cm and a mesh size of 200 meshes.

[0042] In the above technical solution, the white plastic box has the specifications of 37 cm in length × 30 cm in width × 15 cm in height.

[0043] In the above technical solution, the volume of the mineral water bottle is 550 mL, and the position of the mineral water bottle is close to the introduction port of the insect cage.

[0044] In the above technical solution, the sterilized absorbent cotton strip has a length of 28-32 cm, the top of which is 2-3 cm above the bottle mouth and the bottle mouth is completely blocked with absorbent cotton.

[0045] In the above technical solution, the hanging hook has an overall length of 18 cm and is made of iron.

[0046] The technical solution of the present invention has the advantages that: the method for raising oriental fruit flies is simple to operate, saves time, and is easy to maintain the population; the required device is easy to assemble, has low cost, and can be recycled. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 It is a schematic diagram of the overall structure of a breeding cage for stably maintaining a population of Bactrocera dorsalis indoors according to the present invention;

[0048] Figure 2 A schematic diagram of the skeleton of a breeding cage for stably maintaining a population of Bactrocera dorsalis indoors according to the present invention;

[0049] Figure 3 A schematic diagram of a net cover and a connecting rope of a breeding cage for stably maintaining a population of Bactrocera dorsalis indoors according to the present invention;

[0050] Figure 4 It is a schematic diagram of a release port of a breeding cage for stably maintaining a population of Bactrocera dorsalis indoors according to the present invention;

[0051] Figure 5 A schematic diagram of a shielding net of a breeding cage for stably maintaining a population of Bactrocera dorsalis indoors according to the present invention;

[0052] Figure 6 It is a schematic diagram of the operation port of a breeding cage for stably maintaining the population of Bactrocera dorsalis indoors according to the present invention;

[0053] Figure 7 It is a schematic diagram of a mesh sleeve of a breeding cage for stably maintaining a population of Bactrocera dorsalis indoors according to the present invention;

[0054] Figure 8 A schematic diagram of a white plastic box, a mineral water bottle, an absorbent cotton strip and a hanging hook of a breeding cage for stably maintaining a population of Bactrocera dorsalis indoors according to the present invention;

[0055] Among them, 1 is the skeleton; 2 is the net cover; 3 is the connecting rope; 4 is the delivery port; 5 is the shielding net; 6 is the operation port; 7 is the net sleeve; 8 is the white plastic box; 9 is the mineral water bottle; 10 is the absorbent cotton strip; and 11 is the hanging hook. DETAILED DESCRIPTION

[0056] The specific implementation of the technical solution of the present invention is described in detail below, but the present invention is not limited to the following description:

[0057] The present invention provides a method for stably maintaining a population of Bactrocera dorsalis indoors, comprising the following steps:

[0058] (1) Making insect cages:

[0059] A rectangular frame 1 is constructed by using 12 plastic sticks and 8 adapters, and a rectangular mesh cover 2 is constructed by using a gauze net, the length, width and height of the mesh cover are slightly larger than the length, width and height of the frame; one of the four sides of the mesh cover can be opened, and the side is the delivery side, and the delivery side is connected to the top surface, bottom surface and two side surfaces of the mesh cover through a zipper A; the zipper A is unzipped, the frame is placed in the mesh cover to form an insect cage, and then a horizontally arranged rope is placed on the top surface of the frame, and the two ends of the rope are tied to the two long sides or two short sides of the top surface of the frame to form a connecting rope 3; the zipper A is pulled up, and a semicircular delivery port 4 is opened on the delivery side, and the delivery port is equipped with a semicircular shielding net 5, and the shielding net is connected to the delivery port through a zipper B; a circular operating port 6 is opened on the shielding net, and the circular operating port is connected to a cylindrical mesh sleeve 7, and the end of the mesh sleeve is open;

[0060] (2) Preparation of breeding place:

[0061] Put sterilized sand in a white plastic box 8, spread it evenly, and spray double distilled water on the sterilized sand to increase its humidity; then pour double distilled water into a mineral water bottle 9 until it is full, and insert a sterilized absorbent cotton strip 10 into the mineral water bottle to block the bottle mouth; then put the mineral water bottle with the blocked bottle mouth into the white plastic box with sterilized sand; finally, open the zipper B, and put the white plastic box with the mineral water bottle and sterilized sand into the insect cage through the insertion port;

[0062] (3) Production and incubation site:

[0063] Take a clean apple, spray anhydrous ethanol evenly on the surface of the apple and dry it under sterile conditions; use sterilized tweezers to poke holes on the surface of the apple; fix the apple on a hook 11 made of iron wire, and the hook goes through the stem depression to the calyx depression of the apple; then hang the hook 11 on the connecting rope 3 in the insect cage; finally, pull up the zipper B and tighten the operation port;

[0064] (4) Rearing and daily care of the oriental fruit fly:

[0065] Open zipper B, take 60 pairs (60 males and 60 females) of newly emerged orange fruit flies from the population raised in the laboratory, place them in an insect cage containing sterilized sand, mineral water bottles, and apples, and quickly zip up the cage to prevent the orange fruit flies from flying out; observe the water level of double distilled water in the mineral water bottle every 6 days to ensure that the water in the bottle is sufficient for the orange fruit flies; replace apples every 3 days before laying eggs, dissect the replaced apples, and observe whether there are eggs and larvae in the fruit; if there are no eggs and larvae, store the apples at -20℃ for 48 hours and then throw them away. If there are eggs and larvae, place the apples in a new insect cage; hang a punctured apple in the new insect cage 8 days after the mature larvae pupate for the newly emerged orange fruit flies to eat.

[0066] The present invention also provides a breeding cage for the oriental fruit fly that is involved in the above method and is used to stably maintain the population of the oriental fruit fly indoors. The main body is a rectangular frame 1, and the outside of the frame is covered with a rectangular mesh cover 2. The length, width and height of the mesh cover are slightly larger than the length, width and height of the frame. Figure 1-8 As shown:

[0067] The frame has a connecting rope 3 disposed transversely on the top surface, and the two ends of the connecting rope are tied to the two long sides or the two short sides of the top surface of the frame;

[0068] The mesh cover has one side among the four sides that can be opened for delivery, and the delivery side is connected to the top surface, the bottom surface and the two side surfaces of the mesh cover through a zipper A;

[0069] A semicircular delivery opening 4 is provided on the delivery side, and a semicircular shielding net 5 is provided at the delivery opening, and the shielding net is connected to the delivery opening via a zipper B;

[0070] The shielding net is provided with a circular operation opening 6, which is connected to a cylindrical net sleeve 7, the end of which is open and can be tied with a knot or a rope;

[0071] A white plastic box 8 is placed inside the mesh cover, and the white plastic box is also located inside the frame;

[0072] The bottom of the white plastic box is filled with sterilized sand, and a mineral water bottle 9 filled with double distilled water is placed inside, and the sterilized soil covers the bottom of the bottle;

[0073] The mouth of the mineral water bottle is blocked with a sterilized absorbent cotton strip 10;

[0074] The breeding cage is also provided with a hanging hook 11, one end of which is hooked with apples, and the other end is hooked on a connecting rope.

[0075] In the present invention, the skeleton has the following specifications: length 40cm×width 37cm×height 40cm, volume 59200cm 3 .

[0076] In the present invention, the mesh cover has a size of 42 cm in length×39 cm in width×42 cm in height, and a mesh size of 200 meshes.

[0077] In the above technical solution, the length of the connecting rope is 43 cm.

[0078] In the present invention, the shielding net 5 has a mesh size of 200 meshes.

[0079] In the present invention, the operation port 6 has a diameter of 12 cm.

[0080] In the present invention, the cylindrical mesh sleeve 7 has a sleeve length of 18 cm and a mesh size of 200 meshes.

[0081] In the present invention, the white plastic box 8 has a size of 37 cm long×30 cm wide×15 cm high.

[0082] In the present invention, the volume of the mineral water bottle 9 is 550 mL, and the position of the mineral water bottle is close to the introduction port of the insect cage.

[0083] In the present invention, the sterilized absorbent cotton strip 10 has a length of 28-32 cm, the top of which is 2-3 cm above the bottle mouth and the bottle mouth is completely blocked with absorbent cotton.

[0084] In the present invention, the hanging hook 11 has an overall length of 18 cm and is made of iron.

[0085] The present invention will be described below in conjunction with specific embodiments:

[0086] Embodiment 1:

[0087] Preparation of cages for maintaining a stable population of Bactrocera dorsalis indoors:

[0088] ① First, use 8 plastic sticks of 40 cm in length, 4 plastic sticks of 37 cm in length and 8 adapters to build a rectangular frame 1. The specifications of the frame are: length 40 cm × width 37 cm × height 40 cm, and the volume is 59200 cm 3 ; A 20-mesh gauze is then used to construct a rectangular mesh cover 2, the length, width and height of which are slightly larger than those of the frame, and the specifications are 42 cm long × 39 cm wide × 42 cm high; one of the four sides of the mesh cover can be opened, which is the side for placing, and the side for placing is connected to the top surface, bottom surface and two side surfaces of the mesh cover by a zipper A;

[0089] ② Open the zipper A, put the frame into the net cover to form an insect cage, then place a horizontally arranged rope with a length of 43 cm on the top surface of the frame, and tie the two ends of the rope to the two short sides of the top surface of the frame to form a connecting rope 3; make the iron wire into an S-shaped hook 11 with an overall length of 18 cm;

[0090] ③ Pull up the zipper A, and open a semicircular delivery port 4 on the delivery side. The delivery port is equipped with a semicircular 200-mesh shielding net 5, which is connected to the delivery port through the zipper B; a circular operation port 6 with a diameter of 12 cm is opened on the shielding net, and a cylindrical net sleeve 7 with a length of 18 cm and a mesh of 200 mesh is connected to the circular operation port, and the end of the net sleeve is open;

[0091] ④ Place sterilized sand that has passed through a 20-mesh sieve in a white plastic box 8 with a size of 37 cm long × 30 cm wide × 15 cm high, with a thickness of 4-5 cm, and spray double distilled water into the sterilized sand to increase its humidity to 65-75%; then place a 550 mL mineral water bottle 9 filled with double distilled water in the white plastic box, with the sand covering the bottom of the bottle; insert a sterilized, 28-32 cm long absorbent cotton strip 10 into the mineral water bottle to block the bottle mouth, with the top exposed 2-3 cm from the bottle mouth;

[0092] ⑤ Open zipper B, and place the white plastic box containing the mineral water bottle and sterilized sand in the insect cage through the placement port. The position of the mineral water bottle is close to the placement port of the insect cage to facilitate later operations. In this way, a breeding cage for the indoor stable maintenance of the population of the oriental fruit fly is constructed.

[0093] Embodiment 2:

[0094] A breeding cage for indoor stable maintenance of the population of Bactrocera dorsalis prepared by the preparation method of Example 1, the main body is a rectangular frame 1, the outside of the frame is covered with a rectangular mesh cover 2, the length, width and height of the mesh cover are slightly larger than the length, width and height of the frame, the specifications of the frame are: length 40cm×width 37cm×height 40cm, and the volume is 59200cm 3 The specifications of the mesh cover are 42cm long × 39cm wide × 42cm high, and the mesh size is 200 meshes; Figure 1-8 As shown:

[0095] The frame has a connecting rope 3 with a length of 43 cm arranged transversely on the top surface, and the two ends of the connecting rope are tied to the two short sides of the top surface of the frame;

[0096] The mesh cover has one side among the four sides that can be opened for delivery, and the delivery side is connected to the top surface, the bottom surface and the two side surfaces of the mesh cover through a zipper A;

[0097] A semicircular delivery opening 4 is provided on the delivery side, and a semicircular shielding net 5 with a mesh size of 200 is provided at the delivery opening, and the shielding net is connected to the delivery opening via a zipper B;

[0098] The shielding net is provided with a circular operation opening 6 with a diameter of 12 cm, and the circular operation opening is connected with a cylindrical net sleeve 7 with a sleeve length of 18 cm and a mesh size of 200. The end of the net sleeve is open and can be tied with a knot or a rope.

[0099] A white plastic box 8 is placed inside the mesh cover, with specifications of 37cm long×30cm wide×15cm high, and the white plastic box is also located inside the frame;

[0100] The bottom of the white plastic box is filled with sterilized sand, and a mineral water bottle 9 with a volume of 550 mL filled with double distilled water is placed inside, and the sterilized soil covers the bottom of the bottle; the mineral water bottle is located near the opening of the insect cage;

[0101] The mouth of the mineral water bottle is blocked with a sterilized absorbent cotton strip 10 with a length of 28-32 cm, and the top of the strip is exposed 2-3 cm from the mouth of the bottle;

[0102] The breeding cage is also provided with an S-shaped hanging hook 11 made of iron wire with an overall length of 18 cm, one end of the hanging hook is used to hook apples, and the other end is hooked on the connecting rope.

[0103] Embodiment 3:

[0104] A method for stably maintaining the population of Bactrocera dorsalis indoors using the breeding cage of Example 2:

[0105] Take a clean Red Fuji apple with a diameter of about 10 cm, spray anhydrous ethanol evenly on the surface of the apple and dry it under sterile conditions; use sterilized ST-14 tweezers from VETUS Company to poke holes on the surface of the apple; then fix the apple on a hanging hook 11, and the hanging hook runs from the stem depression to the calyx depression of the apple; open the zipper B, hang the hanging hook 11 on the connecting rope 3 in the insect cage, and put a moist white plastic box containing sterilized sand and double distilled water in the insect cage; take 60 pairs (60 males and 60 females) of newly emerged orange fruit flies from the population raised in the laboratory, put them in an insect cage containing sterilized sand, a mineral water bottle, and an apple, quickly zip up the cage, and tighten the cuffs of the net sleeves to prevent the orange fruit flies from flying out.

[0106] Observe the water level of double distilled water in a 550mL mineral water bottle every 6 days to ensure that the water in the bottle is above 380mL; replace apples every 3 days before laying eggs, dissect the replaced apples, and observe whether there are eggs and larvae in the fruit; if there are no eggs and larvae, store the apples at -20℃ for 48 hours and then throw them away. If there are eggs and larvae, place the apples in a new insect cage; hang a punctured apple in the new insect cage 8 days after the mature larvae pupate for the newly emerged orange fruit flies to eat. Record the number of deaths of orange fruit flies every day. As shown in Table 1:

[0107] 1d 2d No. 3d No. 4d No. 5d 6d No. 7d Female 0 0 1 1 1 1 0 male worm 1 1 2 1 0 2 2

[0108] The above examples are only for illustrating the technical concept and technical features of the present invention, and cannot be used to limit the protection scope of the present invention. Any equivalent transformation or modification made according to the essence of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for stably maintaining a population of Bactrocera dorsalis indoors, characterized in that: The following steps are involved: Step 1: Make an insect cage: A rectangular frame (1) is constructed by using 12 plastic sticks and 8 adapters, and a rectangular mesh cover (2) is constructed by using a gauze net, wherein the length, width and height of the mesh cover are slightly larger than the length, width and height of the frame; one of the four side surfaces of the mesh cover can be opened, and the side surface is a delivery side surface, and the delivery side surface is connected to the top surface, the bottom surface and the two side surfaces of the mesh cover through a zipper A; the zipper A is opened, the frame is placed in the mesh cover to form an insect cage, and then a horizontally arranged rope is placed on the top surface of the frame, and the two ends of the rope are tied to the two long sides or the two short sides of the top surface of the frame to form a connecting rope (3); the zipper A is closed, and a semicircular delivery opening (4) is opened on the delivery side surface, and the delivery opening is provided with a semicircular shielding net (5), and the shielding net is connected to the delivery opening through a zipper B; a circular operating opening (6) is opened on the shielding net, and the circular operating opening is connected to a cylindrical mesh sleeve (7), and the end of the mesh sleeve is open; Step 2: Make a feeding place: Sterilized sand is placed in a white plastic box (8) and spread evenly, and double distilled water is sprayed on the sterilized sand to increase its humidity; then double distilled water is poured into a mineral water bottle (9) until it is full, and a sterilized absorbent cotton strip (10) is inserted into the mineral water bottle to block the bottle mouth; then the mineral water bottle with the blocked bottle mouth is placed in the white plastic box containing the sterilized sand; finally, the zipper B is opened, and the white plastic box containing the mineral water bottle and the sterilized sand is placed in the insect cage through the insertion port; Step 3: Make a hatching place: Take a clean apple, spray anhydrous ethanol evenly on the surface of the apple and dry it under sterile conditions; use sterilized tweezers to poke holes on the surface of the apple; fix the apple on a hook (11) made of iron wire, and the hook penetrates from the stem depression to the calyx depression of the apple; then hang the hook (11) on the connecting rope (3) in the insect cage; finally, pull up the zipper B and tighten the operation port; Step 4: Rearing and daily management of the oriental fruit fly: Open zipper B, take 60 pairs of newly emerged orange fruit flies from the population raised in the laboratory, 60 males and 60 females of the 60 pairs of orange fruit flies, put them in an insect cage containing sterilized sand, mineral water bottles, and apples, and quickly zip up the cage to prevent the orange fruit flies from flying out; observe the water level of double distilled water in the mineral water bottle every 6 days to ensure that the water in the bottle is sufficient for the orange fruit flies; replace apples every 3 days before laying eggs, dissect the replaced apples, and observe whether there are eggs and larvae in the fruit; if no eggs and larvae are found, store the apples at -20℃ for 48 hours and then throw them away. If eggs and larvae are found, place the apples in a new insect cage; 8 days after the mature larvae in the new insect cage pupate, hang a pierced apple again for the newly emerged orange fruit flies to eat.

2. The method according to claim 1, characterized in that: In step 1, the 12 plastic sticks are 8 40 cm long and 4 37 cm long, and the skeleton has the following specifications: length 40 cm×width 37 cm×height 40 cm, with a volume of 59200 cm 3 ; the mesh cover (2) has the following specifications: length 42 cm×width 39 cm×height 42 cm, and the mesh size is 200 mesh.

3. The method according to claim 1, characterized in that: In step 1, the length of the connecting rope (3) is 43 cm; the diameter of the operating port (6) is 12 cm; and the length of the cylindrical mesh sleeve (7) is 18 cm.

4. The method according to claim 1, characterized in that: In step 2, the sterilized sand is sieved with a 20-mesh sieve and then placed in a white plastic box with a thickness of 4-5 cm; double distilled water is sprayed into the sterilized sand to increase its humidity to 65-75%.

5. The method according to claim 1, characterized in that: In step 2, the sterilized cotton wool strip has a length of 28-32 cm, the top of which is 2-3 cm above the bottle mouth and the bottle mouth is completely blocked with the cotton wool.

6. The method according to claim 1, characterized in that: In step 2, the volume of the mineral water bottle is 550 mL, the bottom of the bottle is covered with sterilized soil, and the position of the mineral water bottle is close to the opening of the insect cage.

7. The method according to claim 1, characterized in that: In step 2, the white plastic box has the specifications of length 37 cm × width 30 cm × height 15 cm.

8. The method according to claim 1, characterized in that: In step 3, the apple has a diameter of 8-12 cm and is a Red Fuji variety; ST-14 tweezers from VETUS Company are used to pierce the surface of the apple; and the length of the iron wire used to make the hanging hook is 18 cm.

9. The method according to claim 1, characterized in that: In step 4, the double distilled water in the mineral water bottle must not be less than 2 / 3 to prevent the cotton wool on the bottle mouth from drying out.

10. A breeding cage used in the method of claim 1, wherein the main body is a rectangular parallelepiped frame (1), the outside of the frame is covered with a rectangular parallelepiped mesh cover (2), the length, width and height of the mesh cover are slightly larger than the length, width and height of the frame, and the characteristics are: The frame has a connecting rope (3) disposed transversely on the top surface, and the two ends of the connecting rope are tied to the two long sides or the two short sides of the top surface of the frame; The mesh cover has one side among the four sides that can be opened for delivery, and the delivery side is connected to the top surface, the bottom surface and the two side surfaces of the mesh cover through a zipper A; A semicircular delivery opening (4) is provided on the delivery side, and a semicircular shielding net (5) is provided at the delivery opening, and the shielding net is connected to the delivery opening via a zipper B; The shielding net is provided with a circular operating opening (6), the circular operating opening is connected to a cylindrical net sleeve (7), the end of the net sleeve is open and can be tied with a knot or a rope; A white plastic box (8) is placed inside the mesh cover, and the white plastic box is also located inside the frame; The bottom of the white plastic box is filled with sterilized sand, and a mineral water bottle (9) filled with double distilled water is placed inside, with the sterilized sand covering the bottom of the bottle; The mouth of the mineral water bottle is blocked with a sterilized absorbent cotton strip (10); The breeding cage is also provided with a hanging hook (11), one end of which is hooked with apples, and the other end of which is hooked on a connecting rope.

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