A cell collection device for a membrane liquid-based cell slicer

Abstract

The present invention belongs to the field of liquid-based cell preparation, and in particular to a cell collection device for a membrane-type liquid-based cell preparation machine. The following scheme is proposed, which includes a second shell, a first shell is fixedly installed on the top of the second shell, a cylinder is fixedly installed on the upper end of the first shell, a lifting rod is connected to the output end of the cylinder, the lower end of the lifting rod passes through the first shell and extends to the inside of the first shell, and a collection box is fixedly installed, a mounting through hole is provided at the upper end of the second shell, the lower end of the collection box passes through the mounting through hole and extends to the inside of the second shell, two rectangular through holes are symmetrically provided at the left and right ends of the collection box, and filter membranes are fixedly installed on the inner walls of the two rectangular through holes along the vertical direction, a motor is fixedly installed on the right end of the first shell, and a bidirectional screw is connected to the output shaft of the motor. The present invention has good separation effect, convenient collection, high degree of automation, simple operation and high practicality.

Description

Technical Field

[0001] The invention relates to the field of liquid-based cell preparation, in particular to a cell collection device of a membrane-type liquid-based cell preparation machine. Background Art

[0002] The liquid-based cell slide machine can evenly distribute a thin layer of cells on the carrier, overcoming the problems of traditional Pap smear technology, such as too thick materials, overlapping cells, blurred background and poor staining. The liquid-based cell slide machine can reproduce the same specimen, which is more conducive to the accurate diagnosis and screening of pathological cells. This technology helps to screen abnormal cells, improve the sensitivity and specificity of cervical lesion screening, more easily detect early cancer, reduce the incidence of advanced cancer, and reduce the mortality rate of cervical cancer.

[0003] The working principle of the commonly used membrane liquid-based cell slice making machine is to immerse the filter membrane tube or filter membrane cup with a filter membrane installed at the bottom into the preservation solution containing cells, and then create negative pressure in the filter membrane tube / cup through an air pump, suck the preservation solution into the filter membrane tube / cup, so that the cells in the preservation solution are adsorbed on the outside of the filter membrane, and then take out the filter membrane tube / cup, apply appropriate positive pressure to the slide, and transfer the cells to the slide, thereby realizing fast and convenient cell preparation. In the process of collecting cells through the filter membrane, if the air pump running time is not set properly, it is possible to cause the negative pressure in the filter membrane tube to be too high, causing the filter membrane to burst. Or when the cell density in the preservation solution is low, a longer collection time is required. If the negative pressure collection time is set too short, the amount of collected cells will be insufficient. In addition, if the amount of preservation solution is small and the collection time is set too long, the air pump may run idle. A cell collection device for a membrane liquid-based cell slice making machine is proposed. Summary of the invention

[0004] The cell collection device of a membrane liquid-based cell slicer proposed by the present invention solves the problems of poor collection effect, complicated operation and low fault tolerance of the existing cell collection device.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A cell collection device for a membrane-type liquid-based cell preparation machine comprises a second shell, a first shell is fixedly mounted on the top of the second shell, a cylinder is fixedly mounted on the upper end of the first shell, a lifting rod is connected to the output end of the cylinder, a lower end of the lifting rod passes through the first shell and extends into the interior of the first shell, and a collection box is fixedly mounted thereon, a mounting through hole is provided at the upper end of the second shell, a lower end of the collection box passes through the mounting through hole and extends into the interior of the second shell, two rectangular through holes are symmetrically provided at the left and right ends of the collection box, and filter membranes are fixedly mounted on the inner side walls of the two rectangular through holes in a vertical direction, and an electric Machine, the motor output shaft is connected with a bidirectional screw, the left end of the bidirectional screw passes through the first shell and is rotatably connected with the left end inside the first shell, two ball sliders are symmetrically threadedly installed on the bidirectional screw, and scrapers are fixedly installed at the lower ends of the two ball sliders, and two collecting plates are symmetrically fixedly installed at the left and right ends of the collecting box and below the filter membrane, a piston rod is fixedly installed at the middle position of the bottom end of the second shell, the upper end of the piston rod is located inside the collecting box and is fixedly installed with a piston plate, the piston plate is movably arranged inside the collecting box, and the piston plate is in contact with the inner wall of the collecting box, and a door panel is hinged at the front end of the first shell.

[0007] Preferably, the two scrapers are symmetrically arranged just above the left and right end surfaces of the collecting box.

[0008] Preferably, two support rods are symmetrically fixedly installed on the upper end of the second shell, support plates are fixedly installed on the upper ends of the two support rods, and collection boxes are placed on the upper ends of the two support plates.

[0009] Preferably, transparent observation windows are fixedly installed at the rear end of the second shell and the front end of the door panel.

[0010] Preferably, two mounting rods are symmetrically fixedly installed on the left and right ends of the collection box and located below the collection plate, and positioning rods are fixedly installed on one end of the two mounting rods away from each other. Two positioning plates are symmetrically fixedly installed on the left and right ends of the inside of the second shell, and slots are provided on the two positioning plates. The two positioning rods are movably inserted in the two slots respectively.

[0011] Preferably, the left end inside the second shell is fixedly installed and connected with a liquid outlet pipe, and the liquid outlet pipe is provided with a switch valve.

[0012] Preferably, a handle is fixedly mounted on the front end of the door panel, and a sponge cover is provided on the handle.

[0013] Preferably, a rubber base is fixedly mounted on the lower end of the second shell.

[0014] The beneficial effects of the present invention are as follows: by providing a collection box, a filter membrane, a second housing, a collection plate, a positioning rod, a mounting rod, a slot, a positioning plate, a rubber base and a piston rod, etc., cells and cell preservation fluid can be automatically separated, and the separation effect is good and the stability is good; by providing a support rod, a support plate, a collection box, a scraper, a ball slider, a bidirectional screw rod and a motor, etc., the separated cells can be automatically collected, and the collection is convenient and fast, and the degree of automation is high. The present invention has good separation effect, convenient collection, high degree of automation, simple operation and high practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a three-dimensional diagram of a cell collection device of a membrane-type liquid-based cell slicer proposed by the present invention.

[0016] Figure 2 This is a front view of a cell collection device of a membrane-type liquid-based cell slicer proposed by the present invention.

[0017] Figure 3 This is a rear view of a cell collection device of a membrane-type liquid-based cell slide making machine proposed by the present invention.

[0018] Figure 4 The present invention provides a schematic structural diagram of a collection box in a cell collection device of a membrane-type liquid-based cell slicer.

[0019] Numbers in the figure: 1 first shell, 2 collecting box, 3 filter membrane, 4 second shell, 5 collecting plate, 6 positioning rod, 7 mounting rod, 8 slot, 9 positioning plate, 10 rubber base, 11 piston rod, 12 liquid outlet pipe, 13 switch valve, 14 piston plate, 15 mounting through hole, 16 support rod, 17 support plate, 18 collecting box, 19 scraper, 20 ball slider, 21 two-way screw rod, 22 lifting rod, 23 cylinder, 24 motor, 25 handle, 26 door panel, 27 transparent observation window. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0021] Reference Figure 1-4A cell collection device for a membrane-type liquid-based cell preparation machine comprises a second shell 4, a first shell 1 is fixedly mounted on the top of the second shell 4, a cylinder 23 is fixedly mounted on the upper end of the first shell 1, a lifting rod 22 is connected to the output end of the cylinder 23, a lower end of the lifting rod 22 passes through the first shell 1 and extends to the inside of the first shell 1, and a collection box 2 is fixedly mounted, a mounting through hole 15 is provided on the upper end of the second shell 4, a lower end of the collection box 2 passes through the mounting through hole 15 and extends to the inside of the second shell 4, two rectangular through holes are symmetrically provided on the left and right ends of the collection box 2, and a filter membrane 3 is fixedly mounted on the inner side walls of the two rectangular through holes in the vertical direction, a motor 24 is fixedly mounted on the right end of the first shell 1, and the motor 24 is fixedly mounted on the right end of the first shell 1. The output shaft of the machine 24 is connected to a bidirectional screw rod 21, the left end of the bidirectional screw rod 21 passes through the first shell 1, and is rotatably connected to the left end inside the first shell 1, two ball sliders 20 are symmetrically threadedly installed on the bidirectional screw rod 21, and scrapers 19 are fixedly installed at the lower ends of the two ball sliders 20, and two collecting plates 5 are symmetrically fixedly installed at the left and right ends of the collecting box 2 and below the filter membrane 3, a piston rod 11 is fixedly installed in the middle position of the bottom end of the second shell 4, the upper end of the piston rod 11 is located inside the collecting box 2, and a piston plate 14 is fixedly installed, the piston plate 14 is movably arranged inside the collecting box 2, and the piston plate 14 is in contact with the inner wall of the collecting box 2, and a door panel 26 is hinged at the front end of the first shell 1.

[0022] In this embodiment, two scrapers 19 are symmetrically arranged just above the left and right end surfaces of the collection box 2. Through the above design, it is convenient for the scraper 19 to scrape the cells collected on the filter membrane 3. Two support rods 16 are symmetrically fixedly installed on the upper end of the second shell 4. Support plates 17 are fixedly installed on the upper ends of the two support rods 16. Collection boxes 18 are placed on the upper ends of the two support plates 17, which can facilitate the collection of cells on the collection plate 5. Transparent observation windows 27 are fixedly installed on the rear end of the second shell 4 and the front end of the door panel 26, which can facilitate observation of the inside of the second shell 4. Two mounting rods 7 are symmetrically fixedly installed on the left and right ends of the collection box 2 and below the collection plate 5. The two mounting rods 7 are fixed at one end away from each other. A positioning rod 6 is installed, and two positioning plates 9 are symmetrically fixedly installed at the left and right ends of the inside of the second shell 4. Slots 8 are opened on the two positioning plates 9, and the two positioning rods 6 are movably inserted in the two slots 8 respectively. Through the above design, the stability of the collection box 2 when collecting cells can be improved. The left end of the inside of the second shell 4 is fixedly installed and connected with a liquid outlet pipe 12, and a switch valve 13 is arranged on the liquid outlet pipe 12. A handle 25 is fixedly installed at the front end of the door panel 26, and a sponge cover is arranged on the handle 25, which can facilitate the staff to open and close the door panel 26, and then facilitate the collection box 18 to be taken and placed. A rubber base 10 is fixedly installed at the lower end of the second shell 4, which can improve the stability of the present invention.

[0023] The electrical components mentioned in this article are all connected to an external main controller and 220V mains electricity, and the main controller can be a conventional known device for controlling a computer or the like.

[0024] Embodiment: First, open the door panel 26, and then pour the cell preservation solution into the second housing 4 through the installation through hole 15 until the positioning rod 6 is inserted into the positioning plate 9 to lock it, and then start the cylinder 23. The cylinder 23 drives the collection box 2 to descend through the lifting rod 22. The descending collection box 2 enters the second housing 4 through the installation through hole 15. The piston plate 14 enters the collection box 2. First, the cell preservation solution is pressed from the filter membrane 3 to the inside of the collection box 2. The cell preservation solution is filtered onto the filter membrane 3. Then start the cylinder 23. The cylinder 23 drives the collection box 2 to move up through the lifting rod 22. The piston plate 14 can move the second housing 4 upward. The cell preservation fluid inside the shell 4 is sucked into the collection box 2 through the filter membrane 3. When the collection box 2 moves up to above the second shell 4, the cells filtered out from the surface of the filter membrane 3 can be scraped to the upper end of the collection plate 5 by the scraper 19. When the scraper 19 moves to the collection plate 5, the motor 24 is started. The motor 24 drives the bidirectional screw rod 21. The bidirectional screw rod 21 drives the two ball sliders 20 to move in opposite directions, so that the cells on the collection plate 5 can be scraped into the collection box 18. Finally, the collection box 18 is taken out to complete the cell collection. By opening the switch valve 13, the remaining cell preservation fluid in the second shell 4 can be discharged through the liquid outlet pipe 12. By providing a collection box 2, a filter membrane 3, a second housing 4, a collection plate 5, a positioning rod 6, a mounting rod 7, a slot 8, a positioning plate 9, a rubber base 10 and a piston rod 11, etc., it is possible to realize automatic separation of cells and cell preservation fluid, with good separation effect and good stability; by providing a support rod 16, a support plate 17, a collection box 18, a scraper 19, a ball slider 20, a bidirectional screw rod 21 and a motor 24, etc., it is convenient to automatically collect the separated cells, and the collection is convenient and fast with a high degree of automation. The present invention has good separation effect, convenient collection, high degree of automation, simple operation and high practicality.

[0025] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0026] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0027] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A cell collection device for a membrane-based liquid-based cell preparation machine, comprising a second housing (4), characterized in that: The first housing (1) is fixedly mounted on the top of the second housing (4); a cylinder (23) is fixedly mounted on the upper end of the first housing (1); a lifting rod (22) is connected to the output end of the cylinder (23); the lower end of the lifting rod (22) passes through the first housing (1) and extends into the interior of the first housing (1); and a collecting box (2) is fixedly mounted thereon; a mounting through hole (15) is provided on the upper end of the second housing (4); the lower end of the collecting box (2) passes through the mounting through hole (15) and extends into the interior of the second housing (4); two rectangular through holes are symmetrically provided at the left and right ends of the collecting box (2); and filter membranes (3) are fixedly mounted on the inner side walls of the two rectangular through holes in a vertical direction; a motor (24) is fixedly mounted on the right end of the first housing (1); and the output shaft of the motor (24) is connected to a bidirectional screw rod (2 1), the left end of the bidirectional screw rod (21) passes through the first shell (1) and is rotatably connected to the left end inside the first shell (1), two ball sliders (20) are symmetrically threadedly installed on the bidirectional screw rod (21), and the lower ends of the two ball sliders (20) are fixedly installed with scrapers (19), and two collecting plates (5) are symmetrically fixedly installed at the left and right ends of the collecting box (2) and below the filter membrane (3), a piston rod (11) is fixedly installed at the middle position of the bottom end inside the second shell (4), the upper end of the piston rod (11) is located inside the collecting box (2) and is fixedly installed with a piston plate (14), the piston plate (14) is movably arranged inside the collecting box (2), and the piston plate (14) is in contact with the inner wall of the collecting box (2), and a door plate (26) is hinged at the front end of the first shell (1).

2. The cell collection device of a membrane-based liquid-based cell preparation machine according to claim 1, characterized in that: The two scrapers (19) are symmetrically arranged just above the left and right end surfaces of the collecting box (2).

3. The cell collection device of a membrane-based liquid-based cell preparation machine according to claim 1, characterized in that: Two support rods (16) are symmetrically fixedly mounted on the upper end of the second housing (4), support plates (17) are fixedly mounted on the upper ends of the two support rods (16), and collection boxes (18) are placed on the upper ends of the two support plates (17).

4. The cell collection device of a membrane-based liquid-based cell preparation machine according to claim 1, characterized in that: A transparent observation window (27) is fixedly mounted on the rear end of the second housing (4) and the front end of the door panel (26).

5. The cell collection device of a membrane-based liquid-based cell preparation machine according to claim 1, characterized in that: Two mounting rods (7) are symmetrically fixedly installed at the left and right ends of the collection box (2) and located below the collection plate (5); a positioning rod (6) is fixedly installed at one end of the two mounting rods (7) away from each other; two positioning plates (9) are symmetrically fixedly installed at the left and right ends inside the second shell (4); slots (8) are provided on the two positioning plates (9), and the two positioning rods (6) are movably inserted into the two slots (8) respectively.

6. The cell collection device of a membrane-based liquid-based cell preparation machine according to claim 1, characterized in that: The left end of the interior of the second housing (4) is fixedly installed and connected to a liquid outlet pipe (12), and a switch valve (13) is provided on the liquid outlet pipe (12).

7. The cell collection device of a membrane-based liquid-based cell preparation machine according to claim 1, characterized in that: A handle (25) is fixedly mounted on the front end of the door panel (26), and a sponge sleeve is provided on the handle (25).

8. The cell collection device of a membrane-based liquid-based cell preparation machine according to claim 1, characterized in that: A rubber base (10) is fixedly mounted on the lower end of the second housing (4).

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