A device and method for screening and purifying particulate fat

Abstract

The present invention belongs to the field of medical technology, and specifically relates to a device and method for screening and purifying particulate fat. The device includes an upper tank body, an annular intermediate connecting piece and a lower tank body, wherein the upper and lower tank bodies are respectively provided with pipeline interfaces, and the upper and lower tank bodies are respectively detachably connected to the intermediate connecting piece, and a jet ring is detachably connected to the inner ring of the intermediate connecting piece, and a screen is provided above the jet ring, and the screen is detachably connected to the intermediate connecting piece, and a middle pipeline interface leading to the jet ring is also provided on the outer surface of the intermediate connecting piece. The device also includes a bracket, which is horizontally rotatably connected to the intermediate connecting piece, and the bracket is provided with a locking mechanism for limiting the rotation of the intermediate connecting piece. The present invention uses the principle of buoyancy to screen and collect particulate fat, does not use direct mechanical force, and uses shaking and jetting to separate fat particles and ensure the dredging of the screen, so as to efficiently screen fat particles of a specified size.

Description

Technical Field

[0001] The present invention relates to technologies such as fat transplantation, adipose tissue component extraction, and adipose stem cell extraction, belonging to the field of tissue engineering and regenerative medicine, and clinical medicine technology, and specifically to a device and method for screening and purifying particulate fat. Background Art

[0002] Tissue defects caused by trauma, tumors, congenital malformations, and aging are the focus of plastic surgery. Autologous fat particle transplantation is an important technical means currently used in tissue regeneration and repair and medical cosmetology, and has been increasingly widely used in clinical practice. However, the effect of fat transplants is still very limited, and problems such as absorption and necrosis in fat transplantation cannot be avoided. The main reasons are: first, the fat purification method commonly used in fat transplantation is inefficient, causes great damage, and has poor effects; second, the optimal size of fat particles for fat filling in different parts and different amounts is different, and there is currently a lack of effective methods for separating fat particles of different sizes.

[0003] Purification of granular fat is a critical process in fat transplantation. This process can remove a large amount of harmful components in granular fat transplants, such as water, oil, blood components, and fiber cords, which are not conducive to the survival of adipose tissue. Currently, the commonly used methods for purifying granular fat in the world include static method, centrifugation method, cotton pad method, etc. A large number of studies have confirmed that the transplantation effect of granular fat transplants obtained by the cotton pad method is significantly better than that of the centrifugation method and the static method. However, due to adsorption, extrusion, etc., the cotton pad method also causes the greatest loss of fat. So far, a fat purification method that is both less damaging to fat and can effectively remove impurities has not been found.

[0004] The size of fat particles is another important factor for survival after transplantation. After a large number of studies, it is believed that fat cells transplanted within 2mm of vascularized tissue can survive through plasma absorption and early revascularization. Yosh imura proposed the "three-zone" concept of transplantation substitution theory, believing that transplanted fat cells within 300um of the periphery of the fat graft can survive, and the regeneration zone and necrosis zone are located outside 300um from the inside to the outside. Eto et al. further proved that the fat tissue in the latter two zones is mainly differentiated and regenerated by activated adipose stem cells. Therefore, large-diameter granular fat may have marginal necrosis after transplantation, resulting in low fat graft volume retention rate. However, when large-volume fat is used to fill defects in the clinic, the granular fat transplantation obtained with a smaller caliber liposuction needle is not effective, and the incidence of complications is significantly increased. In our previous experiments, it was found that obvious fat droplets were produced when screening granular fat of 0.5mm and below, which indicates that when processing adipose tissue, small-diameter granular fat has weak mechanical resistance and is more likely to rupture. Therefore, we believe that granular fat with a suitable diameter can not only improve the survival rate of fat grafts, but also reduce the loss of granular fat.

[0005] The existing particulate fat filtering devices available on the market are all mechanical pressure type, which force the particulate fat to pass through the screen by applying positive or negative pressure, and all have serious shortcomings: first, the pressure itself causes great damage to the fat cells; second, the particulate fat is forced through the screen and is subjected to huge shear force, which destroys the integrity and vitality of the fat cells; third, the larger particles quickly clog the screen, and a large number of small particles cannot pass through the screen holes, and the efficiency is extremely low; fourth, the use of mechanical scraping to clear the screen further aggravates the damage to the fat cells.

[0006] In summary, how to effectively remove impurities from liposuction and effectively screen fat particles of the required size is a key issue to improve the survival and efficiency of fat transplantation. Existing technologies and equipment cannot obtain fat particles of a set diameter and uniform size without loss and high efficiency. It is necessary to provide a new device and method for screening and purifying fat particles to solve the shortcomings of the existing technology. Summary of the invention

[0007] In order to solve the deficiencies of the prior art, the present invention provides a device and method for screening and purifying particulate fat. The outstanding features of the device and method for screening and purifying particulate fat of the present invention are non-destructive and efficient: the buoyancy principle is used for screening and collection, and direct mechanical force is not used. Shaking and jetting are used to separate fat particles and ensure the dredging of the screen, so fat particles of a specified size can be efficiently screened. Its advantages are: first, it greatly protects the structure of fat particles and reduces damage to fat cells; second, the size of the obtained fat particles is uniform and precisely controllable; third, the filtration efficiency is improved, and waste and loss are reduced; fourth, the whole process is closed and sterile, reducing the risk of contamination; fifth, the process can be repeated to obtain fat particles of various sizes.

[0008] The technical solution provided by the present invention is as follows:

[0009] A particle fat screening and purification device comprises an upper tank body, an annular intermediate connecting piece and a lower tank body which are connected in sequence from top to bottom, an upper pipe interface is arranged at the upper part of the upper tank body, a lower pipe interface is arranged at the lower part of the lower tank body, the lower part of the upper tank body is detachably sealedly connected to the upper part of the intermediate connecting piece, the upper part of the lower tank body is detachably sealedly connected to the lower part of the intermediate connecting piece, a jet ring is detachably connected to the inner circle of the intermediate connecting piece, a screen is arranged above the jet ring, the screen is detachably connected to the intermediate connecting piece, a middle pipe interface leading to the jet ring is also arranged on the outer surface of the intermediate connecting piece, the particle fat screening and purification device also comprises a bracket, the bracket is horizontally rotatably connected to the intermediate connecting piece, the bracket is provided with a locking mechanism for limiting the rotation of the intermediate connecting piece around its horizontal axis every half circle.

[0010] The particle fat screening and purification device provided by the above technical solution uses the physical principle that the screen only allows objects smaller than the aperture size to pass through, so as to filter and separate fat particles of different sizes; two groups of screens with different apertures are used to filter the fat particles separately, first using the screen with a larger aperture and then using the screen with a smaller aperture, so that fat particles of any size between two apertures can be obtained, and oils smaller than the two apertures can be automatically filtered out; the characteristic that the specific gravity of fat tissue and oil is lower than that of water is utilized, and the buoyancy principle is applied to make the fat particles automatically float up in the liquid and pass through the screen to be concentrated in the uppermost layer of the liquid.

[0011] Specifically, the jet ring includes an annular body, and the body is provided with a plurality of guide grooves connecting the inner wall and the outer wall on the upper end surface around the circumference; the guide groove is a linear structure, and the normal angle between the guide groove and the outer wall of the body is 0-45°.

[0012] Furthermore, the main body includes an upper ring portion and a lower ring portion which are coaxially fixedly connected up and down, the inner diameters of the upper ring portion and the lower ring portion are the same, the outer diameter of the upper ring portion is smaller than the outer diameter of the lower ring portion, each of the guide grooves is arranged on the upper end surface of the upper ring portion, a gap is arranged between the outer peripheral wall of the upper ring portion and the intermediate connecting piece, the outer periphery of the lower ring portion is threadedly connected to the inner wall of the intermediate connecting piece, and the middle pipe interface is connected to the gap.

[0013] The above technical solution utilizes the flushing of jet water flow to separate the fat particles adhering to the sieve, thereby avoiding the clogging of the sieve holes; the jet water flow is utilized to continuously mix and move the fat particles and liquid in the chamber, thereby playing a "stirring" role without damaging the fat cells, loosening the adhesion between large and small fat particles, and facilitating the separation and filtration of the fat particles.

[0014] Specifically, the aperture of the sieve can be set to different specifications.

[0015] Specifically, the positioning pin mechanism is a positioning pin, which passes through the bracket and is plugged into it. The intermediate connector is provided with a pair of limit sockets that match the positioning pins respectively. The upper tank body, the intermediate connector and the lower tank body can be horizontally rotated together until the positioning pin is plugged into any one of the limit sockets for locking. A latch is provided between the tank body and the bracket. When the latch is in a locked state, the position of the tank body can be fixed to limit its flipping. The bracket is made of metal, including a base and two pillars. The top of the pillar has a groove corresponding to the rotating shaft of the tank body, which can allow the tank body to rotate and separate the two.

[0016] Specifically, the upper tank body is in an inverted funnel shape; and the lower tank body is in a funnel shape.

[0017] The above technical solution utilizes the unique funnel shape of the tank body to make the filtered granular fat automatically float up under the action of buoyancy and concentrate at the top opening of the funnel-shaped chamber, and lead out through the pipeline, thereby realizing the automatic collection of the granular fat after sorting; by gentle shaking, relative movement between fat particles is generated, thereby reducing the adhesion between fat particles and the screen, and providing floating space for smaller fat particles.

[0018] Specifically, the upper tank body is threadedly connected or snap-fitted to the intermediate connecting piece; the lower tank body is threadedly connected or snap-fitted to the intermediate connecting piece.

[0019] Specifically, the lower portion of the upper tank body and the upper portion of the intermediate connecting piece are sealed and connected via a sealing ring.

[0020] Specifically, the upper portion of the lower tank body is sealed and connected to the lower portion of the intermediate connecting member via a sealing ring.

[0021] Specifically, the upper pipeline interface is provided with an upper valve; the upper valve is connected to a collecting bottle.

[0022] Specifically, the lower pipeline interface is provided with a lower valve.

[0023] Specifically, the middle pipeline interface is provided with a middle valve; the middle valve is connected to a flushing liquid infusion bottle.

[0024] Specifically, a protrusion is provided on the inner periphery of the upper portion of the intermediate connecting member, and the screen is provided between the protrusion and the jet ring.

[0025] Specifically, the intermediate connecting member is provided with a rotating shaft, the rotating shaft is fixedly connected with a rocker arm, and the rocker arm is connected to a motor that drives it to rotate horizontally around the axis of the rotating shaft through a connecting rod. The rotating shaft provided in the middle allows the entire device to swing and flip around the rotating shaft.

[0026] The present invention also provides a method for screening and purifying particulate fat, which uses the particulate fat screening and purification device provided by the present invention to perform screening, comprising the following steps:

[0027] 1) obtaining fresh adipose tissue particles from which large fibers are removed through coarse filtration;

[0028] 2) installing a screen with a first aperture on the particle fat screening and purification device, then opening the lower valve and the upper valve, injecting the fresh adipose tissue particles obtained in step 1) from the lower pipeline interface, then closing the lower valve, opening the middle valve to inject flushing liquid until liquid flows out of the upper pipeline interface, and then closing the middle valve;

[0029] 3) Fixing the particle fat screening and purification device on a shaking device and shaking it, and after starting to shake, continuously or intermittently opening the middle valve to flush it with a flushing liquid;

[0030] 4) Shake until no fat flows out of the upper pipe interface, turn off the shaking device, collect the granular fat and oil after the primary screening in the collecting bottle, then suck out the granular fat after the primary screening, let it stand for a sufficient time, remove the lower layer of water and the upper layer of oil and set aside for use;

[0031] 5) closing all valves, turning the granular fat screening and purification device upside down, i.e., rotating it horizontally by 180°, locking the locking device, opening the upper valve, and then opening the middle valve to inject flushing liquid, and obtaining the first purified granular fat from the upper pipeline interface, wherein the particle size of the first purified granular fat is larger than the first pore size;

[0032] 6) Clean the inside of the granular fat screening and purification device, and repeat steps 2), 3), 4), and 5) in sequence to obtain secondarily purified granular fat, wherein, during the process of repeating step 2), the granular fat screening and purification device is replaced with a screen having a second aperture, and then the first screened granular fat obtained in step 4) is injected from the lower pipe interface, the second aperture is smaller than the first aperture, and the particle size of the second purified granular fat obtained is between the first aperture and the second aperture.

[0033] In the above technical scheme: coarse filtration can remove coarse fibers; in the initial filtration of steps 2), 3), 4), and 5), the screen (aperture 1) is installed according to the set aperture, and the granular fat tissue after coarse filtration is injected into the lower chamber of the tank body from the lower interface, and the two chambers of the tank body are filled with flushing liquid, and the entire device is placed on a shaking device, and flushing liquid is injected through the middle pipe interface. Under the auxiliary action of shaking and jet flow, fat particles and grease smaller than the aperture of the screen automatically float up through the screen under the action of buoyancy and enter the collector; step 6) secondary filtration In the process, the screening and purification device is emptied, and a set screen (aperture 2) is replaced, whose aperture is smaller than the primary filtration screen. The fat particles after the first screening are injected into the lower chamber of the tank body through the lower interface, and the two chambers of the tank body are filled with flushing liquid. The whole device is placed on a shaking table, and flushing liquid is injected through the middle interface. With the assistance of the shaking table swing and the built-in jet device, the fat particles and grease smaller than the aperture of the screen automatically float up through the screen under the action of buoyancy and are removed from the screening device; after the above multiple filtrations, granular fat within the set diameter range can be obtained.

[0034] Furthermore, the method further comprises step 7): repeating step 6) several times, replacing and installing screens with different apertures in each repetition, and passing the sieved granular fat obtained in the previous repetition into the granular fat screening and purification device for purification this time, wherein the apertures of the screens installed in each purification are successively reduced; and the particle size of the purified granular fat obtained in each repetition of step 6) is between the apertures of the screens used in two adjacent repetitions.

[0035] In the above technical solution, after multiple repetitions, fat particles within a specified particle size range can be obtained step by step and accurately.

[0036] Specifically, the shaking device is a shaking table; the shaking device is horizontal shaking. The amplitude and frequency can be adjusted as needed, for example, the frequency is 60-120 times / minute.

[0037] Specifically, the flushing solution is PBS buffer, balanced solution or physiological saline.

[0038] Specifically, in step 3), the middle valve is opened continuously or intermittently to be flushed with a flushing liquid. For example, the middle valve is opened every 1 minute to be flushed with a buffer solution.

[0039] The shaking time from step 3) to step 4) can be set as required, for example, 10-20 minutes.

[0040] Based on the above technical solution, the present invention can also be improved as follows.

[0041] Furthermore, the upper and lower tank bodies can be made of metal and alloy materials, organic chemical materials, glass, etc., and can be directly processed by casting, machining, 3D printing, etc., or manufactured by processes such as inverted molding, casting, extrusion molding, and firing.

[0042] A further beneficial effect of the above method is that different materials and processes can be used to produce devices with different costs according to different usage requirements, such as a one-time screening and purification device.

[0043] Furthermore, the coarse filtering device and method are as follows: an object with multiple holes or multiple protrusions on the surface is placed in the fat suspension for stirring.

[0044] A further beneficial effect of the above is that larger fibrous tissue can be adhered and separated from the particulate fat.

[0045] Furthermore, the coarse filtering device and method are as follows: the particulate fat is allowed to pass through a grid or mesh with larger gaps.

[0046] A further beneficial effect of the above method is that larger fibrous tissue is blocked outside the grid or mesh, thereby separating it from the particulate fat.

[0047] Furthermore, the valve device is a mechanical valve, an electronic valve, or a hydraulic valve.

[0048] The above further beneficial effects are: improving the use efficiency of the valve and reducing the risk of contamination during operation.

[0049] Furthermore, the directions of the plurality of holes of the jet ring are at a certain angle to the normal direction and are directed toward the screen.

[0050] A further beneficial effect of the above is that by controlling the diameter, direction and flow rate of the holes, the flushing effect of the water flow on the screen and the intensity of the stirring effect on the particulate fat suspension can be accurately controlled.

[0051] Furthermore, the sealing ring may be made of an elastomer of chemical materials, and may be located on the side and end surfaces where the upper and lower parts and the middle part of the tank body contact, and the cross-sectional shape may be any geometric shape.

[0052] A further beneficial effect of adopting the above method is: increasing the sealing effect of the tank body.

[0053] Furthermore, the screen can be made by one-time molding, 3D printing, laser punching, mechanical stamping, welding and other processes.

[0054] A further beneficial effect of adopting the above method is to improve the accuracy and mechanical strength of the screen.

[0055] Furthermore, the shaking device and method are: using any mechanical equipment that can generate shaking, including a shaking table.

[0056] The above method has the further beneficial effect of generating relative movement between fat particles, thereby reducing adhesion between fat particles and the screen, and providing floating space for smaller fat particles to avoid clogging of the mesh.

[0057] Furthermore, the shaking device and method are as follows: a rocker arm is directly connected to the rotating shaft of the tank body, connected to the crankshaft through a connecting rod, and then connected to an electric device through a reduction device to drive the tank body to swing.

[0058] A further beneficial effect of adopting the above is: integrating the device and reducing dependence on other equipment.

[0059] The beneficial effects of the device and method for screening and purifying particulate fat of the present invention are:

[0060] The outstanding advantages of the particle fat screening and purification device and method of the present invention are losslessness and high efficiency, which are specifically embodied in:

[0061] 1. Greatly protect the structure of fat particles and reduce damage to fat cells;

[0062] 2. The size of the obtained fat particles is uniform and precisely controllable;

[0063] 3. Improve the filtration efficiency and reduce the waste and loss of particulate fat;

[0064] 4. The whole process is closed and aseptic, reducing the risk of contamination;

[0065] 5. This process can be repeated many times to obtain fat particles of various sizes. BRIEF DESCRIPTION OF THE DRAWINGS

[0066] Figure 1 It is a schematic diagram of the main structure of the particle fat screening and purification device provided by the present invention.

[0067] Figure 2 It is a schematic diagram of the top view of the structure of the device for screening and purifying particulate fat provided by the present invention.

[0068] Figure 3It is a side structural schematic diagram of the particle fat screening and purification device provided by the present invention.

[0069] Figure 4 It is a front view of the jet ring in the particle fat screening and purification device provided by the present invention.

[0070] Figure 5 It is a top view of the jet ring in the particle fat screening and purification device provided by the present invention.

[0071] Figure 6 This is a schematic diagram of the external structure of the device for screening and purifying particulate fat provided by the present invention.

[0072] Figure 7 This is a rendering of the effect of the particulate fat screening and purification method provided by the present invention.

[0073] Attached Figure 1 , 2 , 3, 4, 5, 6, the structure list represented by each number is as follows:

[0074] 1. upper tank body, 2. middle connecting piece, 3. lower tank body, 4. jet ring, 401. guide groove, 402. upper ring part, 403. lower ring part, 5. screen, 6. bracket, 7. positioning pin, 8. rotating shaft. DETAILED DESCRIPTION

[0075] The principles and features of the present invention are described below. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0076] In a specific embodiment, Figure 1 , 2As shown in Figures 3 and 4, the particle fat screening and purification device comprises an upper tank body 1, an annular intermediate connecting piece 2 and a lower tank body 3 which are connected in sequence from top to bottom. An upper pipe interface is provided at the upper part of the upper tank body 1, and a lower pipe interface is provided at the lower part of the lower tank body 3. The upper tank body 1 is detachably connected to the upper part of the intermediate connecting piece 2 at the lower part, and the lower part of the upper tank body 1 and the upper part of the intermediate connecting piece 2 are sealed. The lower tank body 3 is detachably connected to the lower part of the intermediate connecting piece 2 at the upper part, and the lower tank body 3 is sealed to the lower part of the intermediate connecting piece 2 at the upper part. A jet ring 4 is detachably connected to the inner ring of the intermediate connecting piece 2, and a screen 5 is provided above the jet ring 4. The aperture of the screen 5 can be selected in different sizes. The screen 5 is detachably connected to the intermediate connecting piece 2, and a middle pipe interface leading to the jet ring 4 is also provided on the outer surface of the intermediate connecting piece 2. The particle fat screening and purification device also includes a bracket 6, which is horizontally connected to the intermediate connecting member 2 for rotation. The bracket 6 is provided with a locking mechanism that limits the rotation of the intermediate connecting member 2 around its horizontal axis every half turn. The particle fat screening and purification device provided by this technical solution can filter and separate fat particles of different sizes by using the physical principle that the screen only allows objects smaller than the aperture size to pass through; two groups of screens with different apertures are used to filter the fat particles separately, first using the screen with a larger aperture, and then using the screen with a smaller aperture, so that fat particles of any size between two apertures can be obtained, and fats smaller than two apertures can be automatically filtered out; the characteristic that the specific gravity of fat tissue and fat is lower than that of water is used, and the buoyancy principle is applied to make the fat particles automatically float up in the liquid and pass through the screen to be concentrated in the uppermost layer of the liquid.

[0077] In one embodiment, Figure 4 , 5 As shown, the jet ring 4 includes an annular body, and the body is provided with a plurality of guide grooves 401 connecting the inner wall and the outer wall thereof on the upper end face around the circumference; the guide grooves 401 are linear structures, and the normal angle between the guide grooves 401 and the outer wall of the body is 45°. The body includes a coaxial upper ring portion 402 and a lower ring portion 403, the inner diameters of the upper ring portion 402 and the lower ring portion 403 are the same, the outer diameter of the upper ring portion 402 is smaller than the outer diameter of the lower ring portion 403, each guide groove 401 is provided on the upper end face of the upper ring portion 402, a gap is provided between the peripheral wall of the upper ring portion 402 and the intermediate connector 2, the lower ring portion 403 is threadedly connected to the inner wall of the intermediate connector 2 at the outer circumference, and the middle pipe interface connects the gap. Based on this technical solution, the fat particles adhering to the sieve can be detached by flushing with a jet of water, thereby avoiding clogging of the sieve holes; the fat particles and liquid in the chamber are continuously mixed and moved by the jet of water, which has a "stirring" effect without damaging the fat cells, loosens the adhesion between large and small fat particles, and is beneficial to the separation and filtration of the fat particles.

[0078] In one embodiment, the locking mechanism is a positioning pin 7, which penetrates the bracket 6 and is plugged therein, and the intermediate connector 2 is provided with a pair of limit holes respectively matched with the positioning pin 7, and the upper tank body 1, the intermediate connector 2 and the lower tank body 3 can be horizontally rotated together until the positioning pin 7 is plugged into any one of the limit holes for locking. Based on this technical solution, the device can be flipped and positioned.

[0079] In one embodiment, the upper tank body 1 is in an inverted funnel shape; the lower tank body 3 is also in a funnel shape. This technical solution utilizes the unique funnel shape of the tank body to automatically float the filtered fat particles under the action of buoyancy and concentrate at the top opening of the funnel-shaped chamber, and lead out through the pipeline, thereby realizing the automatic collection of the fat particles after sorting; by using gentle shaking, relative movement between fat particles is generated, thereby reducing the adhesion between fat particles and the screen, and providing floating space for smaller fat particles.

[0080] In one embodiment, the upper pipe interface is provided with an upper valve; the upper valve is connected to a collection bottle. The lower pipe interface is provided with a lower valve. The middle pipe interface is provided with a middle valve; the middle valve is connected to a flushing liquid infusion bottle. The intermediate connector 2 is provided with a rotating shaft 8, and the rotating shaft 8 is fixedly connected to a rocker arm and connected to a motor that drives it to rotate horizontally around the axis of the rotating shaft through a connecting rod. Based on this technical solution, the control of the equipment operation process can be easily realized.

[0081] In one embodiment, a protrusion is provided on the inner circumference of the upper part of the intermediate connecting member 2, and the screen 5 is provided between the protrusion and the jet ring 4. Based on this technical solution, the installation and replacement of the screen 5 can be conveniently realized.

[0082] In one embodiment, the upper tank body 1 is threadedly connected to the intermediate connecting piece 2; the lower tank body 3 is threadedly connected to the intermediate connecting piece 2. Based on this technical solution, the tank body and the intermediate connecting piece can be easily assembled and disassembled.

[0083] In one embodiment, the method for screening and purifying particulate fat specifically comprises the following steps:

[0084] 1) Take 50 ml of fresh granular adipose tissue, let it stand for 15 minutes, gently stir it several times with two or more porous liposuction needles, discard the fiber strands adhering to it, add flushing solution and wash it three times to obtain granular adipose tissue after removing the crude fiber;

[0085] 2) Install a 3mm pore size screen on the particle fat screening and purification device, and tighten the tank. Connect the upper interface of the tank to the collection bottle with an infusion tube, and connect the middle interface to the infusion bottle with flushing solution with an infusion tube. Then inject fresh fat tissue particles from the lower pipeline interface, close the lower valve, open the middle valve, inject flushing solution until liquid flows out of the upper pipeline interface, and then close the middle valve;

[0086] 3) Fix the particle fat screening and purification device on the shaker, turn on the shaker, run it at a speed of 100 times / minute, open the middle port valve for 10 seconds every 1 minute, and continue for 10 minutes until no fat flows out of the upper port infusion tube;

[0087] 4) Shake until no fat flows out of the upper pipe interface, turn off the shaking device, collect the sieved granular fat in a collection bottle, then suck out the sieved granular fat, let it stand for 15 minutes, remove the lower layer of water and the upper layer of grease for later use;

[0088] 5) The granular fat screening and purification device is turned upside down and fixed, and then the inside of the device is cleaned with a flushing liquid from the middle valve, and the purified granular fat is collected at the pipe interface located at the top, and the particle size thereof is greater than 3 mm;

[0089] 6) Clean the inside of the fat particle screening and purification device, and repeat steps 2), 3), 4), and 5) in sequence to obtain the second purified fat particles, the diameter of the second purified fat particles is greater than 2 mm and less than 3 mm. In the process of repeating step 2), replace the fat particle screening and purification device with a 2 mm pore size screen, and then inject the first screened fat particles from the lower pipe interface.

[0090] 7) Repeat steps 5) and 6) several times, and replace the sieves with different apertures each time, and the apertures are 1mm, 0.5mm, 0.2mm, and 0.1mm in sequence, and the sieved granular fat obtained in the previous repetition is passed into the granular fat screening and purification device for purification, wherein the aperture of the sieve installed in each repetition is reduced in sequence. Finally, granular fat of different sizes with diameters of 1-2mm, 0.5-1mm, 0.2-0.5mm, and 0.1-0.2mm are obtained.

[0091] The effects of the purified granular fat obtained in each step are as follows Figure 3 In each test tube, the white part is the purified granular fat.

[0092] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A device for screening and purifying particulate fat, characterized in that: The invention comprises an upper tank body (1), an annular intermediate connecting piece (2) and a lower tank body (3) which are connected in sequence from top to bottom, wherein the upper part of the upper tank body (1) is provided with an upper pipe interface, and the lower part of the lower tank body (3) is provided with a lower pipe interface, the lower part of the upper tank body (1) is detachably sealedly connected to the upper part of the intermediate connecting piece (2), the upper part of the lower tank body (3) is detachably sealedly connected to the lower part of the intermediate connecting piece (2), a jet ring (4) is detachably connected to the inner ring of the intermediate connecting piece (2), and a screen (5) is provided above the jet ring (4), wherein the upper part of the upper tank body (1) is provided with an upper pipe interface, and the lower part of the lower tank body (3) is provided with a lower pipe interface, wherein the upper part of the upper tank body (1) is detachably sealedly connected to the upper part of the intermediate connecting piece (2), and the upper part of the lower tank body (3) is provided with a lower pipe interface, wherein the upper part of the upper tank body (1) is provided with an upper pipe interface, and the lower part of the lower tank body (3) is provided with a lower pipe interface, wherein the upper part of the lower tank body (3) is provided with a lower pipe interface, wherein the lower part of the upper tank body (1) is provided with an upper pipe interface, and the lower part of the lower tank body (3) is provided with an upper ... The screen (5) is detachably connected to the intermediate connecting member (2); a middle pipe interface leading to the jet ring (4) is also provided on the outer surface of the intermediate connecting member (2); the particle fat screening and purification device further comprises a bracket (6); the bracket (6) is horizontally rotatably connected to the intermediate connecting member (2); the bracket (6) is provided with a locking mechanism for limiting the rotation of the intermediate connecting member (2) around its horizontal axis every half circle; the upper tank body (1) is in the shape of an inverted funnel; the lower tank body (3) is in the shape of a funnel; the aperture of the screen (5) can be set to different specifications; The jet ring (4) comprises an annular body, wherein the body is provided with a plurality of guide grooves (401) connecting the inner wall and the outer wall thereof on the upper end surface in the circumferential direction; the guide grooves (401) are linear structures, and the normal angle between the guide grooves and the outer wall of the body is 0-45°; The locking mechanism is a positioning pin (7), which passes through the bracket (6) and is plugged into the bracket (6). The intermediate connecting member (2) is provided with a pair of limit holes respectively matched with the positioning pin (7) at the upper and lower sides. The upper tank body (1), the intermediate connecting member (2) and the lower tank body (3) can be horizontally rotated together until the positioning pin (7) is plugged into any one of the limit holes for locking.

2. The device for screening and purifying particulate fat according to claim 1, characterized in that: The body comprises an upper ring portion (402) and a lower ring portion (403) which are coaxially fixedly connected up and down, the inner diameters of the upper ring portion (402) and the lower ring portion (403) are the same, the outer diameter of the upper ring portion (402) is smaller than the outer diameter of the lower ring portion (403), each of the guide grooves (401) is arranged on the upper end surface of the upper ring portion (402), a gap is arranged between the outer peripheral wall of the upper ring portion (402) and the intermediate connecting piece (2), the outer periphery of the lower ring portion (403) is threadedly connected to the inner wall of the intermediate connecting piece (2), and the middle pipe interface is connected to the gap.

3. The device for screening and purifying particulate fat according to any one of claims 1 to 2, characterized in that: The upper tank body (1) is threadedly connected or snap-fitted to the intermediate connecting piece (2); the lower tank body (3) is threadedly connected or snap-fitted to the intermediate connecting piece (2); The lower part of the upper tank body (1) and the upper part of the intermediate connecting piece (2) are sealed and connected via a sealing ring; The upper part of the lower tank body (3) and the lower part of the intermediate connecting piece (2) are sealed and connected via a sealing ring; The upper pipeline interface is provided with an upper valve; the upper valve is connected to a collecting bottle; The lower pipeline interface is provided with a lower valve; The middle pipe interface is provided with a middle valve; the middle valve is connected to a flushing liquid infusion bottle; The upper inner periphery of the intermediate connecting member (2) is provided with a protrusion, and the screen (5) is arranged between the protrusion and the jet ring (4).

4. The device for screening and purifying particulate fat according to claim 3, characterized in that: The intermediate connecting member (2) is provided with a rotating shaft, the rotating shaft is fixedly connected to a rocker arm, and the rocker arm is connected to a motor that drives it to rotate horizontally around the axis of the rotating shaft through a connecting rod.

5. A method for screening and purifying particulate fat, using the particulate fat screening and purification device according to claim 3 or 4 for screening, characterized in that: The following steps are involved: 1) obtaining fresh adipose tissue particles from which large fibers are removed through coarse filtration; 2) installing a screen (5) with a first aperture on the particle fat screening and purification device, opening the upper valve and the lower valve, injecting the fresh fat tissue particles obtained in step 1) from the lower pipeline interface, closing the lower valve, then opening the middle valve to inject flushing liquid until liquid flows out of the upper pipeline interface, and then closing the middle valve; 3) Fixing the particle fat screening and purification device on a shaking device and shaking it, and after starting to shake, continuously or intermittently opening the middle valve to flush it with a flushing liquid; 4) Shake until no fat flows out of the upper pipe interface, turn off the shaking device, collect the granular fat and grease after the primary screening in the collecting bottle, then suck out the granular fat after the primary screening, let it stand, remove the lower layer of water and the upper layer of grease for standby use; 5) closing all valves, turning the granular fat screening and purification device upside down, locking the locking device, opening the upper valve, and then opening the middle valve to inject flushing liquid, and obtaining the first purified granular fat from the upper pipeline interface, wherein the particle size of the first purified granular fat is larger than the first pore size; 6) Clean the inside of the granular fat screening and purification device, and repeat steps 2), 3), 4), and 5) in sequence to obtain secondarily purified granular fat, wherein, during the process of repeating step 2), the granular fat screening and purification device is replaced with a screen (5) having a second aperture, and then the first screened granular fat obtained in step 4) is injected from the lower pipe interface, wherein the second aperture is smaller than the first aperture; and the particle size of the secondarily purified granular fat is between the first aperture and the second aperture.

6. The method for screening and purifying particulate fat according to claim 5, characterized in that: The method further comprises step 7): repeating step 6) several times, replacing and installing a sieve (5) with a different aperture in each repetition, and passing the sieved granular fat obtained in the previous repetition into the granular fat screening and purification device for purification, wherein the aperture of the sieve (5) installed in each purification is successively reduced; and the particle size of the purified granular fat obtained in each repetition of step 6) is between the apertures of the sieve (5) used in two adjacent repetitions.

7. The method for screening and purifying particulate fat according to any one of claims 5 to 6, characterized in that: The shaking device is a shaking table; the shaking device is in a horizontal shaking mode; The flushing solution is PBS buffer, balanced solution or physiological saline.

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