A tissue sectioning device for making microscope specimens

Abstract

The present invention provides a tissue slicing device for making microscope specimens, which belongs to the field of laboratory tool technology. It includes a rotating cap, a screw, a top block, a limiting cylinder and a positioning column, one end of the screw is fixedly connected to the rotating cap, the screw is threadedly connected to the top block, the top block is rotatably connected to the positioning column, the limiting cylinder is rotatably connected to the rotating cap, the limiting cylinder is sleeved on the top block, the limiting cylinder and the top block are splined, one end of the positioning column has an avoidance hole for allowing the screw to be inserted, the other end of the positioning column is provided with a notch that runs through the circumference and end face of the positioning column, the notch has a clamping block that is rotatably connected to the positioning column, the clamping block includes a rotating part and two spring pieces arranged on the rotating part, and a clamping groove is provided between the two spring pieces; when the clamping block is located outside the notch, the two spring pieces are inclined at an angle of 5 to 10°, and when the clamping block is located inside the notch, the two spring pieces are parallel to each other. The present invention has the advantages of simple slicing operation, high precision, high safety, etc.

Description

Technical Field

[0001] The invention belongs to the technical field of laboratory tools and relates to a tissue slicing device for making microscope specimens. Background Art

[0002] When observing the tissue structure of biological samples through a microscope, the biological tissue needs to be sliced ​​and then pasted (sometimes stained) to make glass slides for microscopic observation. The thickness of the tissue slices is at the micron level. Manual slicing is not only difficult to control the thickness and inconvenient to operate, but also poses a safety hazard.

[0003] In the prior art, paraffin sectioning is usually used for better sectioning, but the process is complicated, especially in summer, paraffin is easy to soften and needs to be frozen and then quickly processed, which seriously affects the portability of the sectioning, especially when students in the laboratory perform related operations, there is great inconvenience. Summary of the invention

[0004] The purpose of the present invention is to provide a tissue slicing device for making microscope specimens in view of the above-mentioned problems existing in the prior art. The technical problem to be solved by the present invention is how to improve the slicing accuracy and slicing safety.

[0005] The object of the present invention can be achieved through the following technical scheme: a tissue slicing device for making microscope specimens, characterized in that it includes a rotating cap, a screw, a top block, a limiting cylinder and a positioning column, one end of the screw is fixedly connected to the rotating cap, the screw is threadedly connected to the top block, the top block is rotatably connected to the positioning column, the limiting cylinder is rotatably connected to the rotating cap, the limiting cylinder is sleeved on the top block, the limiting cylinder and the top block are splined, one end of the positioning column has an avoidance hole allowing the screw to be inserted, the other end of the positioning column is provided with a notch that runs through the circumference and end face of the positioning column, the notch has a clamping block rotatably connected to the positioning column, the clamping block includes a rotating part and two spring pieces arranged on the rotating part, and a clamping groove is provided between the two spring pieces; when the clamping block is located outside the notch, the two spring pieces are inclined at an angle of 5 to 10°, and when the clamping block is located in the notch, the two spring pieces are parallel to each other.

[0006] Furthermore, the middle portion of the inner side surface of the spring sheet has an arc-shaped groove.

[0007] Furthermore, a buffer sheet is adhered to the inner side of the spring sheet.

[0008] Furthermore, the peripheral surface of the positioning column has scale markings.

[0009] The top block and the positioning column, as well as the limiting cylinder and the rotating cap are rotatably connected through the structure of the slot and the convex rib, that is, the convex rib is inserted into the slot by means of a snap connection, so that the two rotatably connected are limited.

[0010] Rotating the rotating cap can realize translation of the top block and the positioning column along the axis of the limiting cylinder, so that the positioning column can be extended and retracted in the limiting cylinder.

[0011] Slicing steps: rotate the clamping block to disengage from the notch, reset the spring sheet, and make the clamping groove space between the two spring sheets larger, and clamp the processed tissue sample between the two spring sheets, with part of the tissue sample located on the positioning column and part of it clamped by the spring sheet; rotate the clamping block into the notch, and under the pressure of the inner wall of the notch, the openings of the two spring sheets are close to each other, so that the tissue sample is clamped, and the clamping block is also positioned in the notch at the same time; rotate the rotating cap to move the positioning column to an appropriate position along the limiting cylinder, and cut the tissue part extending out of the upper end face of the positioning column with a blade to make the fracture of the tissue sample smooth. At the same time, read the scale, continue to rotate the rotating cap, so that the positioning column extends out to the height of the thickness required for slicing, and cut the sample with the blade again to complete the slicing of the tissue.

[0012] When the tissue is cut by the blade, the blade cuts flatly along the cross section of the limiting cylinder to ensure the uniformity and accuracy of the slice thickness.

[0013] The buffer sheet can increase the range of sample clamping thickness and also protect the sample.

[0014] The setting of the groove enables the slicing device to be suitable for slicing rod-shaped samples such as plant stems, and through the avoidance of the groove and the pressing of the buffer sheet, it is suitable for clamping rod-shaped samples within a certain diameter range.

[0015] The slicing device has the characteristics of convenient operation, easy sample disassembly and assembly, simple structure, high slicing accuracy and safe operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a three-dimensional structural schematic diagram of the slicing device.

[0017] Figure 2 yes Figure 1 Exploded diagram of .

[0018] Figure 3 yes Figure 1 Schematic diagram of the structure after removing the limiting cylinder.

[0019] Figure 4 It is a three-dimensional structural schematic diagram of the positioning column.

[0020] In the figure, 1, rotating cap; 2, screw; 3, top block; 4, limiting cylinder; 5, positioning column; 51, notch; 52, spring piece; 53, clamping groove; 54, groove; 55, rotating part; 56, buffer sheet; 6, scale mark; 7, clamping block. DETAILED DESCRIPTION

[0021] The following are specific embodiments of the present invention and the accompanying drawings to further describe the technical solution of the present invention, but the present invention is not limited to these embodiments.

[0022] like Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, it includes a rotating cap 1, a screw rod 2, a top block 3, a limiting cylinder 4 and a positioning column 5, one end of the screw rod 2 is fixedly connected to the rotating cap 1, the screw rod 2 is threadedly connected to the top block 3, the top block 3 is rotatably connected to the positioning column 5, the limiting cylinder 4 is rotatably connected to the rotating cap 1, the limiting cylinder 4 is sleeved on the top block 3, the limiting cylinder 4 and the top block 3 are splined, one end of the positioning column 5 has an avoidance hole allowing the screw rod 2 to be inserted, the other end of the positioning column 5 is provided with a notch 51 that runs through the circumference and end surface of the positioning column 5, the notch 51 has a clamping block 7 rotatably connected to the positioning column 5, the clamping block 7 includes a rotating portion 55 and two spring sheets 52 arranged on the rotating portion 55, and a clamping groove 53 is provided between the two spring sheets 52; when the clamping block 7 is located outside the notch 51, the two spring sheets 52 are inclined at an angle of 5 to 10°, and when the clamping block 7 is located in the notch 51, the two spring sheets 52 are parallel to each other.

[0023] The middle of the inner side surface of the spring piece 52 has an arc-shaped groove 54 , a buffer sheet 56 is adhered to the inner side surface of the spring piece 52 , and the peripheral surface of the positioning column 5 has a scale mark 6 .

[0024] The top block 3 and the positioning column 5, as well as the limiting cylinder 4 and the rotating cap 1, are rotatably connected through the structure of the slot and the convex rib, that is, the convex rib is inserted into the slot by means of a snap connection, so that the two rotatably connected are limited.

[0025] Rotating the rotating cap 1 can realize translation of the top block 3 and the positioning column 5 along the axis of the limiting cylinder 4 , so that the positioning column 5 can be extended and retracted in the limiting cylinder 4 .

[0026] Slicing steps: rotate the clamping block 7 to disengage the clamping block 7 from the notch 51, and the spring sheet 52 is reset, so that the space of the clamping groove 53 between the two spring sheets 52 is larger, and the processed tissue sample is clamped between the two spring sheets 52, and the tissue sample is partially located on the positioning column 5, and partially clamped by the spring sheet 52; rotate the clamping block 7 to the notch 51, and under the pressure of the inner wall of the notch 51, the openings of the two spring sheets 52 are close to each other, so that the tissue sample is clamped, and the clamping block 7 is also positioned in the notch 51 at the same time; rotate the rotating cap 1 to move the positioning column 5 to an appropriate position along the limiting cylinder 4, and cut the tissue part extending out of the upper end surface of the positioning column 5 by a blade to make the fracture of the tissue sample smooth. At the same time, read the scale, continue to rotate the rotating cap 1, so that the positioning column 5 extends outward to the height of the thickness required for slicing, and cut the sample again by the blade to complete the slicing of the tissue.

[0027] When the tissue is cut by the blade, the blade cuts flatly along the cross section of the limiting cylinder 4 to ensure the uniformity and accuracy of the slice thickness.

[0028] The buffer sheet 56 can increase the range of sample clamping thickness and also protect the sample.

[0029] The provision of the groove 54 enables the slicing device to be suitable for slicing rod-shaped samples such as plant stems. Through the avoidance of the groove 54 and the pressing of the buffer sheet 56, it is suitable for clamping rod-shaped samples within a certain diameter range.

[0030] The slicing device has the characteristics of convenient operation, easy sample disassembly and assembly, simple structure, high slicing accuracy and safe operation.

[0031] The specific embodiments described herein are merely examples of the spirit of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in similar ways, but they will not deviate from the spirit of the present invention or exceed the scope defined by the appended claims.

Claims

1. A tissue sectioning device for preparing microscope specimens, characterized in that: The invention comprises a rotating cap (1), a screw rod (2), a top block (3), a limiting cylinder (4) and a positioning column (5), wherein one end of the screw rod (2) is fixedly connected to the rotating cap (1), the screw rod (2) is threadedly connected to the top block (3), the top block (3) is rotatably connected to the positioning column (5), the limiting cylinder (4) is rotatably connected to the rotating cap (1), the limiting cylinder (4) is sleeved on the top block (3), the limiting cylinder (4) and the top block (3) are spline-connected, one end of the positioning column (5) is provided with an avoidance hole for allowing the screw rod (2) to be inserted, and the positioning column (5) The other end of the positioning column (5) is provided with a notch (51) which penetrates the circumference and end surface of the positioning column (5); the notch (51) contains a clamping block (7) rotatably connected to the positioning column (5); the clamping block (7) comprises a rotating portion (55) and two spring sheets (52) arranged on the rotating portion (55); a clamping groove (53) is provided between the two spring sheets (52); when the clamping block (7) is located outside the notch (51), the two spring sheets (52) are inclined at an angle of 5 to 10 degrees; when the clamping block is located inside the notch (51), the two spring sheets (52) are parallel to each other.

2. A tissue sectioning device for preparing microscope specimens according to claim 1, characterized in that: The middle portion of the inner side surface of the spring piece (52) has an arc-shaped groove (54).

3. A tissue sectioning device for preparing microscope specimens according to claim 1 or 2, characterized in that: A buffer sheet (56) is adhered to the inner side of the elastic sheet (52).

4. A tissue sectioning device for preparing microscope specimens according to claim 1 or 2, characterized in that: The peripheral surface of the positioning column (5) is provided with scale marks (6).

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