Zinc-rich candida tropicalis strain and use thereof

Abstract

The present invention relates to a zinc-rich candida tropicalis strain and use thereof. The strain has strong zinc enrichment capacity, high growth rate and high yield, is used for preparing a zinc-rich yeast feed additive, improves a reproductive performance of sows, increases the zinc content in colostrum of the sows, reduces morbidity of piglets and promotes growth. The candida tropicalis strain UDY-12 is bred by utilizing an ultraviolet mutation technology and utilizing a microbial genetic breeding technology and a microbial fermentation technology according to a tolerance characteristic of the yeast strain to zinc. The strain has capability of converting inorganic zinc into yeast zinc during a high-biomass fermentation, has strong zinc enrichment capacity, high growth rate and high yield, can be used for preparing a zinc-rich yeast feed additive product, has wide raw material source, simple and convenient operation, short fermentation period, has high organic zinc content, is safe, efficient and non-toxic, supplements yeast zinc to sows, improves a reproductive performance of the sows, improves zinc content in colostrum of the sows, reduces piglet morbidity and promotes growth.

Description

technical field

[0001] The invention relates to the technical field of microbial fermentation, in particular to a zinc-rich Candida tropicalis strain and its application. Background technique

[0002] Zinc is an essential trace element for animal growth and development, and plays an important role in maintaining animal health and normal physiological functions. Studies have shown that zinc can participate in the secretion and composition of various enzymes and functional proteins in animals. Zinc also participates in the development of animal immune organs, affects the specific and non-specific immune functions of animals, and plays an important role in maintaining the normal immune function of the body and preventing the invasion of pathogenic microorganisms. etc. are of great significance. Zinc is also closely related to many basic physiological and biochemical activities of animals. It not only participates in the metabolism of DNA, RNA, protein, sugar, lipid, minerals a...

Examples

Embodiment 1

[0022] The application method of a strain of zinc-rich Candida tropicalis strain UDY-12 in the preparation of zinc-rich yeast feed additive of the present invention comprises the following steps:

[0023] (1) Preparation of seed solution: insert Candida tropicalis strain UDY-12 into Zn 2+ Erlenmeyer flasks with plate medium, Zn 2 + The mass content is 500μg / mL, cultured at 28°C for 36h, and then inserted with Zn 2+ In the Erlenmeyer flask of YEPD medium, place on a shaker, the shaker speed is 180r / min, and cultivate at 28°C for 60h to obtain a fermentation culture seed solution;

[0024] The Zn 2+ is zinc sulfate;

[0025] (2) Preparation of fermentation culture: Add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 30g / L to make the zinc content reach 300μg / mL, add 2g of potassium dihydrogen phosphate, and use wort medium to set The capacity is up to 1000mL, divided into conical flasks, 100mL per bottle, sterilized at 120°C for 15 minutes,...

Embodiment 2

[0028] The application method of a strain of zinc-rich Candida tropicalis strain UDY-12 in the preparation of zinc-rich yeast feed additive of the present invention comprises the following steps:

[0029] (1) Preparation of seed solution: insert Candida tropicalis strain UDY-12 into Zn 2+Erlenmeyer flasks with plate medium, Zn 2 + The mass content is 500μg / mL, cultured at 28°C for 36h, and then inserted with Zn 2+ In the Erlenmeyer flask of YEPD medium, place on a shaker, the shaker speed is 180r / min, and cultivate at 28°C for 60h to obtain a fermentation culture seed liquid;

[0030] The Zn 2+ is zinc oxide;

[0031] (2) Preparation of fermentation culture: Add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 50g / L to make the zinc content reach 500μg / mL, add 2g of potassium dihydrogen phosphate, and use wort medium to set The capacity is up to 1000mL, divided into conical flasks, 100mL per bottle, sterilized at 120°C for 15min, when the ...

Embodiment 3

[0034] The application method of a strain of zinc-rich Candida tropicalis strain UDY-12 in the preparation of zinc-rich yeast feed additive of the present invention comprises the following steps:

[0035] (1) Preparation of seed solution: insert Candida tropicalis strain UDY-12 into Zn 2+ Erlenmeyer flasks with plate medium, Zn 2 + The mass content is 500μg / mL, cultured at 28°C for 36h, and then inserted with Zn 2+ In the Erlenmeyer flask of YEPD medium, place on a shaker, the shaker speed is 180r / min, and cultivate at 28°C for 60h to obtain a fermentation culture seed liquid;

[0036] The Zn 2+ is zinc sulfate;

[0037] (2) Preparation of fermentation culture: Add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 70g / L to make the zinc content reach 800μg / mL, add 2g of potassium dihydrogen phosphate, and use wort medium to set The capacity is up to 1000mL, divided into Erlenmeyer flasks, 100mL per bottle, sterilized at 120°C for 15 minutes...

technical field

[0001] The invention relates to the technical field of microbial fermentation, in particular to a zinc-rich Candida tropicalis strain and its application. Background technique

[0002] Zinc is an essential trace element for animal growth and development, and plays an important role in maintaining animal health and normal physiological functions. Studies have shown that zinc can participate in the secretion and composition of various enzymes and functional proteins in animals. Zinc also participates in the development of animal immune organs, affects the specific and non-specific immune functions of animals, and plays an important role in maintaining the normal immune function of the body and preventing the invasion of pathogenic microorganisms. etc. are of great significance. Zinc is also closely related to many basic physiological and biochemical activities of animals. It not only participates in the metabolism of DNA, RNA, protein, sugar, lipid, minerals a...

Examples

Embodiment 1

[0022] The application method of a strain of zinc-rich Candida tropicalis strain UDY-12 in the preparation of zinc-rich yeast feed additive of the present invention comprises the following steps:

[0023] (1) Preparation of seed solution: insert Candida tropicalis strain UDY-12 into Zn 2+ Erlenmeyer flasks with plate medium, Zn 2 + The mass content is 500μg / mL, cultured at 28°C for 36h, and then inserted with Zn 2+ In the Erlenmeyer flask of YEPD medium, place on a shaker, the shaker speed is 180r / min, and cultivate at 28°C for 60h to obtain a fermentation culture seed solution;

[0024] The Zn 2+ is zinc sulfate;

[0025] (2) Preparation of fermentation culture: Add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 30g / L to make the zinc content reach 300μg / mL, add 2g of potassium dihydrogen phosphate, and use wort medium to set The capacity is up to 1000mL, divided into conical flasks, 100mL per bottle, sterilized at 120°C for 15 minutes,...

Embodiment 2

[0028] The application method of a strain of zinc-rich Candida tropicalis strain UDY-12 in the preparation of zinc-rich yeast feed additive of the present invention comprises the following steps:

[0029] (1) Preparation of seed solution: insert Candida tropicalis strain UDY-12 into Zn 2+Erlenmeyer flasks with plate medium, Zn 2 + The mass content is 500μg / mL, cultured at 28°C for 36h, and then inserted with Zn 2+ In the Erlenmeyer flask of YEPD medium, place on a shaker, the shaker speed is 180r / min, and cultivate at 28°C for 60h to obtain a fermentation culture seed liquid;

[0030] The Zn 2+ is zinc oxide;

[0031] (2) Preparation of fermentation culture: Add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 50g / L to make the zinc content reach 500μg / mL, add 2g of potassium dihydrogen phosphate, and use wort medium to set The capacity is up to 1000mL, divided into conical flasks, 100mL per bottle, sterilized at 120°C for 15min, when the ...

Embodiment 3

[0034] The application method of a strain of zinc-rich Candida tropicalis strain UDY-12 in the preparation of zinc-rich yeast feed additive of the present invention comprises the following steps:

[0035] (1) Preparation of seed solution: insert Candida tropicalis strain UDY-12 into Zn 2+ Erlenmeyer flasks with plate medium, Zn 2 + The mass content is 500μg / mL, cultured at 28°C for 36h, and then inserted with Zn 2+ In the Erlenmeyer flask of YEPD medium, place on a shaker, the shaker speed is 180r / min, and cultivate at 28°C for 60h to obtain a fermentation culture seed liquid;

[0036] The Zn 2+ is zinc sulfate;

[0037] (2) Preparation of fermentation culture: Add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 70g / L to make the zinc content reach 800μg / mL, add 2g of potassium dihydrogen phosphate, and use wort medium to set The capacity is up to 1000mL, divided into Erlenmeyer flasks, 100mL per bottle, sterilized at 120°C for 15 minutes...