A Zinc-rich Candida tropicalis strain and its application

Abstract

The invention relates to a zinc-enriched Candida tropicalis strain and its application, which can effectively solve the problem that the strain has strong zinc-enriching ability, fast growth rate and high yield, and is used to prepare zinc-enriched yeast feed additives and improve the reproductive performance of sows. Increase the zinc content in the colostrum of sows to reduce the problem of piglet disease and growth promotion. The invention utilizes the microbial genetic breeding technology and the microbial fermentation technology, and according to the tolerance characteristic of the yeast strain to zinc, and utilizes the ultraviolet mutagenesis technology to select a strain of Candida tropicalis UDY-12. It has the ability to convert inorganic zinc into yeast zinc. The strain has strong zinc-enriching ability, fast growth rate and high yield. It can prepare zinc-enriched yeast feed additive products. It has a wide range of raw material sources, simple operation and short fermentation period. It has high organic zinc content, is safe, efficient and non-toxic. It can supplement yeast zinc to sows, improve the reproductive performance of sows, increase the zinc content in colostrum of sows, reduce the incidence of piglets and promote growth.

Description

technical field

[0001] The invention relates to the technical field of microbial fermentation, in particular to a zinc-rich Candida tropicalis strain and its application. Background technique

[0002] Zinc is a trace element necessary for animal growth and development, and plays an important role in maintaining animal health and normal physiological functions. Studies have shown that zinc can participate in the secretion and composition of various enzymes and functional proteins in animals. Zinc is also involved in the development of immune organs in animals, affecting the performance of specific and non-specific immune functions in animals, maintaining normal immune function in the body and preventing the invasion of pathogenic microorganisms. etc. are of great significance. Zinc is also closely related to many basic physiological and biochemical activities of animals. It not only participates in the metabolism of DNA, RNA, protein, carbohydrates, lipids, minerals and vita...

Examples

Embodiment 1

[0022] The application method of a zinc-rich Candida tropicalis strain UDY-12 of the present invention in the preparation of a zinc-rich yeast feed additive comprises the following steps:

[0023] (1) Preparation of seed solution: The Candida tropicalis strain UDY-12 was inserted into the solution containing Zn 2+ In the Erlenmeyer flask of the plate medium, Zn 2+ The mass content was 500 μg / mL, cultured at 28 °C for 36 h, and then inserted with Zn 2+ The YEPD medium in the Erlenmeyer flask was placed on a shaker, and the shaker speed was 180r / min, and cultured at 28°C for 60h to obtain a fermented cultured seed liquid;

[0024] the Zn 2+ is zinc sulfate;

[0025] (2) Preparation of fermentation culture: add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 30 g / L to make the zinc element content reach 300 μg / mL, add 2 g of potassium dihydrogen phosphate, and use the wort medium to determine The volume is up to 1000mL, and it is divided into...

Embodiment 2

[0028] The application method of a zinc-rich Candida tropicalis strain UDY-12 of the present invention in the preparation of a zinc-rich yeast feed additive comprises the following steps:

[0029] (1) Preparation of seed solution: The Candida tropicalis strain UDY-12 was inserted into the solution containing Zn 2+ In the Erlenmeyer flask of the plate medium, Zn 2+The mass content was 500 μg / mL, cultured at 28 °C for 36 h, and then inserted with Zn 2+ The YEPD medium was placed in a Erlenmeyer flask, placed on a shaker, and the shaker speed was 180r / min, and cultured at 28°C for 60h to obtain a fermented cultured seed liquid;

[0030] the Zn 2+ is zinc oxide;

[0031] (2) Preparation of fermentation culture: add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 50 g / L to make the zinc element content reach 500 μg / mL, add 2 g of potassium dihydrogen phosphate, and use the wort medium to determine The volume is up to 1000mL, and it is divided i...

Embodiment 3

[0034] The application method of a zinc-rich Candida tropicalis strain UDY-12 of the present invention in the preparation of a zinc-rich yeast feed additive comprises the following steps:

[0035] (1) Preparation of seed solution: The Candida tropicalis strain UDY-12 was inserted into the solution containing Zn 2+ In the Erlenmeyer flask of the plate medium, Zn 2+ The mass content was 500 μg / mL, cultured at 28 °C for 36 h, and then inserted with Zn 2+ The YEPD medium was placed in a Erlenmeyer flask, placed on a shaker, and the shaker speed was 180r / min, and cultured at 28°C for 60h to obtain a fermented cultured seed liquid;

[0036] the Zn 2+ is zinc sulfate;

[0037] (2) Preparation of fermentation culture: add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 70 g / L to make the zinc element content reach 800 μg / mL, add 2 g of potassium dihydrogen phosphate, and use the wort medium to determine The volume is 1000mL, and it is divided into...

technical field

[0001] The invention relates to the technical field of microbial fermentation, in particular to a zinc-rich Candida tropicalis strain and its application. Background technique

[0002] Zinc is a trace element necessary for animal growth and development, and plays an important role in maintaining animal health and normal physiological functions. Studies have shown that zinc can participate in the secretion and composition of various enzymes and functional proteins in animals. Zinc is also involved in the development of immune organs in animals, affecting the performance of specific and non-specific immune functions in animals, maintaining normal immune function in the body and preventing the invasion of pathogenic microorganisms. etc. are of great significance. Zinc is also closely related to many basic physiological and biochemical activities of animals. It not only participates in the metabolism of DNA, RNA, protein, carbohydrates, lipids, minerals and vita...

Examples

Embodiment 1

[0022] The application method of a zinc-rich Candida tropicalis strain UDY-12 of the present invention in the preparation of a zinc-rich yeast feed additive comprises the following steps:

[0023] (1) Preparation of seed solution: The Candida tropicalis strain UDY-12 was inserted into the solution containing Zn 2+ In the Erlenmeyer flask of the plate medium, Zn 2+ The mass content was 500 μg / mL, cultured at 28 °C for 36 h, and then inserted with Zn 2+ The YEPD medium in the Erlenmeyer flask was placed on a shaker, and the shaker speed was 180r / min, and cultured at 28°C for 60h to obtain a fermented cultured seed liquid;

[0024] the Zn 2+ is zinc sulfate;

[0025] (2) Preparation of fermentation culture: add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 30 g / L to make the zinc element content reach 300 μg / mL, add 2 g of potassium dihydrogen phosphate, and use the wort medium to determine The volume is up to 1000mL, and it is divided into...

Embodiment 2

[0028] The application method of a zinc-rich Candida tropicalis strain UDY-12 of the present invention in the preparation of a zinc-rich yeast feed additive comprises the following steps:

[0029] (1) Preparation of seed solution: The Candida tropicalis strain UDY-12 was inserted into the solution containing Zn 2+ In the Erlenmeyer flask of the plate medium, Zn 2+The mass content was 500 μg / mL, cultured at 28 °C for 36 h, and then inserted with Zn 2+ The YEPD medium was placed in a Erlenmeyer flask, placed on a shaker, and the shaker speed was 180r / min, and cultured at 28°C for 60h to obtain a fermented cultured seed liquid;

[0030] the Zn 2+ is zinc oxide;

[0031] (2) Preparation of fermentation culture: add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 50 g / L to make the zinc element content reach 500 μg / mL, add 2 g of potassium dihydrogen phosphate, and use the wort medium to determine The volume is up to 1000mL, and it is divided i...

Embodiment 3

[0034] The application method of a zinc-rich Candida tropicalis strain UDY-12 of the present invention in the preparation of a zinc-rich yeast feed additive comprises the following steps:

[0035] (1) Preparation of seed solution: The Candida tropicalis strain UDY-12 was inserted into the solution containing Zn 2+ In the Erlenmeyer flask of the plate medium, Zn 2+ The mass content was 500 μg / mL, cultured at 28 °C for 36 h, and then inserted with Zn 2+ The YEPD medium was placed in a Erlenmeyer flask, placed on a shaker, and the shaker speed was 180r / min, and cultured at 28°C for 60h to obtain a fermented cultured seed liquid;

[0036] the Zn 2+ is zinc sulfate;

[0037] (2) Preparation of fermentation culture: add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 70 g / L to make the zinc element content reach 800 μg / mL, add 2 g of potassium dihydrogen phosphate, and use the wort medium to determine The volume is 1000mL, and it is divided into...