A macrocirculatory cell interception device, a fluid circulation pump and a downward-probing shaking table

Abstract

The present invention relates to the technical field of cell retention devices, and in particular to a macrocirculation cell retention device, a fluid circulation pump and a downward-probing shaker. The downward-probing shaker of the present invention comprises: a shaker body; a rocking plate on the top of the rocking plate body is installed on an upper load-bearing plate; the four corners of the upper load-bearing plate are respectively installed on the upper ends of four downward-probing columns, and a column fixing frame is installed in the middle of the four downward-probing columns; the lower ends of the four downward-probing columns are respectively installed on the bottom plate of the rocking plate; the bottom plate of the rocking plate is installed with a sliding sleeve and a sleeve locking screw, and the sleeve locking screw is installed outside the center line of the sliding sleeve; the sliding sleeve is slidably installed with an openable and closable heat-insulating tank body. The present invention has outstanding substantial characteristics and significant progress, which is conducive to popularization and application.

Description

Technical Field

[0001] The invention relates to the technical field of cell retention devices, and in particular to a macrocirculation cell retention device, a fluid circulation pump and a downward-probing shaking table. Background Art

[0002] At present, the world's biotechnology pharmaceutical industry is developing rapidly and has achieved great success. The expression and production of various types of diagnostic and therapeutic monoclonal antibodies, vaccines, growth factors and other biologically active proteins through in vitro culture of animal cells has a very broad market application prospect. Since the perfusion process has the advantages of high quality and high efficiency when large-scale suspension culture of animal cells, the key equipment in the perfusion process - the cell retention device has become the most important bioprocess equipment. Because without this equipment, the perfusion process cannot be realized, and the high efficiency of the cell retention device largely determines the production scale, product cost and overall efficiency of biotechnology pharmaceutical companies.

[0003] At present, the mainstream cell retention devices most used in the world, in addition to using hollow fiber tangential flow filtration technology, all use the unique "one pump and one push" reciprocating motion of the diaphragm pump to make the cell mixture flow back and forth. During the reciprocating flow of the cell mixture, the supernatant is extracted by using a peristaltic pump in the outer cavity of the hollow fiber membrane tube, realizing the key step of perfusion process cell cultivation - extracting the supernatant and retaining the cells in the reaction tank at the same time. Due to the characteristics of the hollow fiber membrane structure, the above method can retain 100% of the cells in the reaction tank. It is also this diaphragm pump that causes the cell mixture to change direction at a high frequency (one reciprocation takes two seconds) and also changes the liquid pressure at the same frequency, causing damage to the cells.

[0004] In addition, in such a flow environment, on the one hand, because the direction of the cell mixture flow is parallel to the center line of the hollow fiber tube, the cell mixture is drawn in and out in parallel to the center line of the hollow fiber tube, but the flow direction is opposite. When the peristaltic pump extracts the supernatant, the area of ​​the outer cavity of the hollow fiber tube is negatively pressurized, so that some cells in the cell mixture flowing in the hollow fiber membrane tube also flow out of the tube together with the supernatant due to the negative pressure of the outer cavity of the hollow fiber membrane tube. When the cells reach the micropores on the inner wall of the hollow fiber membrane tube, a small amount of cells are blocked on the micropores, and are not carried down by the mixed liquid flowing downward in the tube. In this way, accumulation occurs over time, and gradually most of the micropores on the inner wall of the hollow fiber membrane tube are blocked by cells. The supernatant cannot be extracted, causing the cell retention device to fail. To continue the perfusion process culture, a new device must be replaced. Since the device is expensive, it will greatly increase the cost.

[0005] The information disclosed in this background technology section is only intended to deepen the understanding of the overall background technology of the present invention, and should not be regarded as acknowledging or suggesting in any form that the information constitutes the prior art already known to those skilled in the art. Summary of the invention

[0006] The object of the present invention is to provide a macrocirculatory cell interception device, a fluid circulation pump and a downward-probing shaking table to solve the technical problems existing in the prior art.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a downward-reaching rocking table, which comprises: a rocking table body;

[0009] The rocking plate on the top of the rocking table body is installed on the upper load-bearing plate; the four corners of the upper load-bearing plate are respectively installed on the upper ends of four downward-expanding columns, and a column fixing frame is installed in the middle of the four downward-expanding columns; the lower ends of the four downward-expanding columns are respectively installed on the rocking plate bottom plate; the rocking plate bottom plate is installed with a sliding sleeve and a sleeve locking screw, and the sleeve locking screw is installed on the outside of the center line of the sliding sleeve; the upper part of the sliding sleeve is installed with an insulation tank body bottom plate and an openable insulation tank body, and installation openings for pipelines to extend vertically downward are reserved at the bottom of the insulation tank body and the rocking plate bottom plate.

[0010] As a further technical solution, the openable and closable insulation tank body includes: an insulation tank body bottom plate, a front half of the insulation tank and a rear half of the insulation tank; the insulation tank body bottom plate is installed with the front half of the insulation tank and the rear half of the insulation tank; the bottom of the insulation tank body bottom plate is provided with a sliding seat for being sleeved on the sliding sleeve; the front half of the insulation tank and the rear half of the insulation tank are connected on the rotating side through the insulation tank body hinge; the front half of the insulation tank and the rear half of the insulation tank are provided with locking bolts on the opening and closing side.

[0011] As a further technical solution, a stopper is provided on the sliding path of the insulation tank body on the rocking plate bottom plate, and an L-shaped abutment is provided on the outer side of the insulation tank body bottom plate; the stopper is adapted to the L-shaped abutment on the outer side of the insulation tank body bottom plate.

[0012] As a further technical solution, four tank positioning pins are arranged between the front half and the rear half of the insulation tank. The four tank positioning pins not only have the function of positioning the front and rear halves together, but also enable the front and rear halves of the insulation tank to bear the weight together after being combined.

[0013] By adopting the above technical solution, the present invention has the following beneficial effects:

[0014] The downward-probing shaking table in the present invention can meet the fluid mechanics requirements of the circulating pump and has better effects than the existing traditional shaking tables. Because the traditional shaking table has a shaking table body (shaking table driving mechanism part) arranged at its lower part, due to structural limitations, there is no space for the pipeline to extend vertically downward in the middle position of the shaking table, and the pipeline must be arranged in a bend. From the perspective of fluid mechanics, this is obviously not as good as the downward-probing shaking table in this embodiment.

[0015] In the second aspect, the present invention provides a fluid circulation pump, which includes: a double-layer hard plastic shake pot, a stirring pot and the above-mentioned downward-probing shaker; the double-layer hard plastic shake pot is arranged in an openable and closable heat-insulating tank body; the upper water outlet of the stirring pot and the two return water outlets at the bottom of the inner tank of the double-layer hard plastic shake pot are connected by two return water silicone hoses; the lower water inlet of the stirring pot and the outer tank bottom water outlet at the bottom of the outer tank of the double-layer hard plastic shake pot are connected by a water outlet silicone hose; a stirring shaft is arranged in the stirring tank, a stirring paddle is installed on the stirring shaft, and a motor is installed on the end of the stirring shaft.

[0016] As a further technical solution, the double-layer hard plastic shaking can comprises: an outer can, an inner can and a can cover; an outer can bottom water outlet is arranged at the center of the bottom of the outer can; the upper part of the outer can is in the shape of a conical can body, and the lower part of the outer can is in the shape of a barrel can body; the outer can is symmetrically provided with two water inlet convex parts at the position of its upper conical can body; four positioning columns are arranged at the bottom of the outer can; the outer can bottom water outlet at the bottom of the outer can can extend vertically downward from the mounting holes reserved in the bottom plate of the heat-insulating can body and the bottom plate of the shaking plate; two water return ports are symmetrically arranged at the bottom of the inner can; The upper part is in the shape of a conical tank body, and the lower part of the inner tank is in the shape of a barrel tank body; the inner tank is symmetrically provided with an inner tank water outlet at the position of the upper conical tank body; an inner tank water outlet tangential flow baffle is provided on one side edge of the inner tank water outlet; an inner tank water outlet lower horizontal flow baffle is provided on the bottom side edge of the inner tank water outlet; the two return water inlets at the bottom of the inner tank can extend vertically downward from the mounting holes reserved at the bottom plate of the thermal insulation tank body and the bottom plate of the rocking plate; the tank cover and the flange of the outer tank are sealed and connected by a sealing rubber ring of the outer tank flange; and a number of air filters are provided on the tank cover.

[0017] As a further technical solution, the inner tank is inserted into the inner part of the outer tank, and a positioning seat is provided at the bottom of the inner tank, and the positioning seat is inserted and connected with the positioning column at the bottom of the outer tank.

[0018] As a further technical solution, any one of the water outlet silicone hose and the two water return silicone hoses is provided with a hollow fiber microfiltration membrane column; the hollow fiber microfiltration membrane column is connected to the stirring tank via a fixing clamp.

[0019] As a further technical solution, the fluid circulation pump includes: a movable bracket; the movable bracket includes: a bracket, a movable bracket bottom plate, and four movable bracket wheels; the top of the bracket is provided with a pipe clamp, the pipe clamp is used to pass through the downward-probing rocking table support shaft, and the rocking table body is installed on the downward-probing rocking table support shaft; the lower part of the bracket is provided with a movable bracket bottom plate; the bottom of the bracket is provided with four movable bracket wheels.

[0020] By adopting the above technical solution, the present invention has the following beneficial effects:

[0021] During the cell retention process, the fluid circulation pump of the present invention allows the cell mixture to flow in a single direction in the large circulation loop inside and outside the stirring tank and the double-layer hard plastic shaking tank, with a stable flow rate, no frequency, high-speed repeated changes in direction and pressure, and very little cell damage (because animal cells themselves have no cell walls, only a thin cell membrane, and a very small shear force can break the cell membrane and cause cell death). Moreover, during the cell retention process, due to the oscillating movement of the mixed liquid in the double-layer hard plastic shaking tank, the water splashes and water droplets are raised, which greatly increases the gas-liquid ratio surface area in the shaking tank, thereby greatly increasing the dissolved oxygen level and speed, and also greatly improving the speed of removing harmful gases such as carbon dioxide and ammonia from the mixed liquid, and has the functions of oxygenation and purification of harmful gases. At present, the existing cell retention devices in the world do not have these two functions.

[0022] In the third aspect, the present invention also provides a large circulating cell retention device, which includes: a supernatant extraction and backwashing system and the above-mentioned fluid circulation pump; the supernatant extraction and backwashing system includes: a hollow fiber microfiltration membrane column, a peristaltic pump, a diaphragm pump assembly, an insulation tank, a supernatant turnover bag, a supernatant storage bag and a supernatant storage tank; the upper part of the hollow fiber microfiltration membrane column is provided with a supernatant extraction outlet, and the lower part of the hollow fiber microfiltration membrane column is provided with a supernatant backwashing inlet; one end of the peristaltic pump hose of the peristaltic pump is connected to the supernatant extraction outlet, and the other end of the peristaltic pump hose of the peristaltic pump is connected to the upper part of the supernatant turnover bag, and the lower part of the supernatant turnover bag is connected to the diaphragm pump assembly; the middle part of the supernatant turnover bag is connected to the supernatant storage bag in the supernatant storage tank; the diaphragm pump assembly is connected to the supernatant backwashing inlet.

[0023] By adopting the above technical solution, the present invention has the following beneficial effects:

[0024] In the cell retention process of the present invention, the cell mixture flows in a large circulation loop inside and outside the stirring tank and the double-layer hard plastic shaking tank in a single direction, with a stable flow rate, no frequency, and high-speed repeated changes in direction and pressure, resulting in very little cell damage (because animal cells themselves have no cell walls, only a thin cell membrane, and a very small shear force can break the cell membrane and cause cell death).

[0025] When the macrocirculation cell interception device of the present invention extracts the supernatant, the cell concentration of the cell mixture is always the same as the cell concentration of the cell mixture in the stirring tank. Extracting the supernatant does not increase the concentration, and extraction is very easy.

[0026] When the backwashing system of the macrocirculation cell retention device of the present invention flushes the cells blocked in the micropores of the hollow fiber membrane tube wall, it can be flushed clean every time without any residue. There is no problem of long-term accumulation. The reason is that the backwashing is in the direction of the outer normal of the micropores of the hollow fiber membrane tube, and the pressure is flushed from the outside of the hollow fiber membrane tube to the inside of the tube. In this way, the direction of the impact force is accurate and it is easy to flush out all the cells blocked in the micropores, and flow out of the lower end of the hollow fiber microfiltration membrane column together with the cell mixture flowing from top to bottom in the hollow fiber membrane tube. Moreover, only a small amount of backwashing liquid is used, and the flushing time is extremely short. This is a special advantage of the backwashing system in the present invention.

[0027] During the cell trapping process, the present invention greatly increases the surface area of ​​gas-liquid ratio in the shaking tank due to the oscillating motion of the mixed liquid in the tank, which raises water splashes and water droplets, thereby greatly increasing the dissolved oxygen level and speed, and also greatly improving the speed of removing harmful gases such as carbon dioxide and ammonia from the mixed liquid, and has the functions of increasing oxygen and purifying harmful gases. At present, the existing cell trapping devices in the world do not have these two functions.

[0028] In the present invention, since the volume of the shaking pot can be greatly increased, the diameter of the circulation pipeline can also be greatly increased, and the shaking speed can also be greatly increased, the result is that the flow rate in the large circulation loop can reach a large amount and the flow rate can also reach a high amount. For example: the volume of the shaking pot is 20 liters, the rotation speed is 80 revolutions per minute, and the flow measurement is 4 times the volume, that is, 80 liters per minute. Moreover, the volume of the shaking pot of the present invention can be enlarged to 200 liters or even 500 liters, which far exceeds the flow rate of existing cell retention devices in the world. In this way, the greater the circulation flow rate, the higher the dissolved oxygen level and the faster the dissolved oxygen speed. The present invention can better meet the needs of large-scale animal cell culture with large volume and high density. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0030] Figure 1 A side view of the downward-probing shaking table provided in the first embodiment of the present invention;

[0031] Figure 2A top view of the downward-probing shaking table provided in the first embodiment of the present invention;

[0032] Figure 3 A top view of the in-situ state of the downward-probing shaking table provided in the first embodiment of the present invention;

[0033] Figure 4 A top view of the downward-reaching shaking table provided in the first embodiment of the present invention with the heat preservation tank body pulled out;

[0034] Figure 5 A top view of the downward-reaching shaking table provided in the first embodiment of the present invention with the heat-insulating tank body opened and the double-layer hard plastic shaking tank loaded;

[0035] Figure 6 A top view of the downward-reaching shaking table provided in the first embodiment of the present invention with the heat preservation tank body closed, the heat preservation tank body pushed back and locked in the working position;

[0036] Figure 7 A side view of a fluid circulation pump provided in Embodiment 1 of the present invention;

[0037] Figure 8 A side view of a double-layer hard plastic shake can provided in Example 1 of the present invention;

[0038] Fig. 9 A top view of a double-layer hard plastic shake can provided in Example 1 of the present invention;

[0039] Fig.10 A side view of a movable bracket provided in Embodiment 1 of the present invention;

[0040] Fig.11 A top view of the movable bracket provided in the first embodiment of the present invention;

[0041] Fig.12 A side view of the macrocirculatory cell retention device provided in Example 1 of the present invention;

[0042] Fig.13 A side view of the supernatant extraction and backwashing system provided in Example 1 of the present invention. DETAILED DESCRIPTION

[0043] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] The specific implementation of the present invention is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the present invention, and is not used to limit the present invention. Embodiment 1

[0045] Combination Figure 1 , Figure 2 As shown, the first embodiment provides a downward-protruding rocking table, which includes: a rocking table body 202; the rocking table body 202 is installed on a downward-protruding rocking table support shaft 220. The rocking plate 208 on the top of the rocking table body 202 is installed on the upper load-bearing plate 210; the four corners of the upper load-bearing plate 210 are respectively installed on the upper ends of four downward-expanding columns 214, and the middle parts of the four downward-expanding columns 214 are installed with a column fixing frame 216; the lower ends of the four downward-expanding columns 214 are respectively installed on the rocking table bottom plate 218; the rocking table bottom plate 218 is installed with a sliding sleeve 320 and a sleeve locking screw 322, and the sleeve locking screw 322 is installed outside the center line of the sliding sleeve 320; the sliding sleeve 320 is slidably installed with an openable and closable insulation tank body, and an installation opening is reserved between the bottom of the insulation tank body and the rocking table bottom plate 218 for the pipeline to extend vertically downward, which meets the fluid mechanics requirements of the circulating pump and has better effect than the existing traditional rocking tables. Because the traditional shaking table has a shaking table body (shaking table drive box part) at its lower part, due to structural limitations, there is no space for the pipeline to extend vertically downward in the middle of the shaking table, and the pipeline has to be set in a bend. From the perspective of fluid mechanics, this is obviously not as good as the downward-protruding shaking table in this embodiment.

[0046] In this embodiment, the specific form of the rocking table body 202 is not limited and can refer to the prior art.

[0047] For example: the rocking table body 202 includes: a rocking table drive box (eccentrically installed, with the eccentric distance 206 shown in the figure as a reference); a drive motor is installed in the rocking table drive box; a small pulley is installed on the power output shaft of the drive motor; the small pulley is connected to the large pulley through a belt tensioning connection; the large pulley is installed on the active eccentric shaft; the upper end of the active eccentric shaft is connected to the upper rocking plate; the upper rocking plate is connected to four sets of driven shafts; the upper rocking plate is connected to the rocking plate 208 by bolts, and the rocking plate 208 is installed on the upper bearing plate 210. It can be seen that in the downward-exploring rocking table in this embodiment, the upper bearing plate 210 is fixed on the rocking plate 208, and four downward-exploring columns 214 are downwardly installed under the upper bearing plate 210, and the lower end of the downward-exploring columns 214 is equipped with a rocking plate bottom plate 218. A column fixing frame 216 is installed on the upper half of the four columns to ensure the rigidity and stability of the frame; a sliding sleeve 320, a sleeve locking screw 322 and a stopper 312 are installed on the rocker bottom plate 218, and an insulation tank bottom plate 340 is installed on the top of the two sliding sleeves 320, and an insulation tank body that can be divided into two halves is installed thereon. At this point, the equipment is installed.

[0048] Combination Figures 1 to 6As shown, in the present embodiment, preferably, the openable and closable heat-insulating tank body comprises: a heat-insulating tank body bottom plate 340, a heat-insulating tank front half 301 and a heat-insulating tank rear half 300; the heat-insulating tank body bottom plate 340 is installed with the heat-insulating tank front half 301 and the heat-insulating tank rear half 300; the bottom of the heat-insulating tank body bottom plate 340 is provided with a sliding seat 321 for being sleeved on the sliding sleeve 320; the heat-insulating tank front half 301 and the heat-insulating tank rear half 300 are connected on the rotating side through the heat-insulating tank body hinge 330; the heat-insulating tank front half 301 and the heat-insulating tank rear half 300 are provided with locking bolts 350 on the opening and closing side.

[0049] In this embodiment, preferably, the bottom plate 340 of the heat-insulating tank body is provided with a U-shaped installation opening.

[0050] Correspondingly, the rocker bottom plate 218 is also provided with a U-shaped mounting opening.

[0051] At the same time, a space for accommodating the double-layer hard plastic shake pot 400 is formed between the front half 301 of the heat preservation pot and the rear half 300 of the heat preservation pot. The maximum opening and closing angle between the front half 301 of the heat preservation pot and the rear half 300 of the heat preservation pot can be set to 79°.

[0052] In this embodiment, preferably, the rocking plate bottom plate 218 is provided with a stopper 312 on the sliding path of the insulation tank body, and the outer side of the insulation tank body bottom plate 340 has an L-shaped stopper; the stopper 312 is adapted to the L-shaped stopper on the outer side of the insulation tank body bottom plate 340.

[0053] In this embodiment, preferably, four tank positioning pins 303 are provided between the front half 301 and the rear half 300 of the heat preservation tank. Embodiment 2

[0054] This second embodiment provides a fluid circulation pump based on the above-mentioned first embodiment.

[0055] Combination Figures 7 to 11 As shown, the fluid circulation pump includes: a double-layer hard plastic shaking pot 400, a stirring pot 102 and the downward-probing shaking table 200 described in the first embodiment.

[0056] In this embodiment, the double-layer hard plastic shake pot 400 is arranged in an insulated tank body that can be opened and closed; the upper water outlet of the stirring pot is connected to the two return water ports 420 at the bottom of the inner tank 405 of the double-layer hard plastic shake pot 400 through two return water silicone hoses 421; the lower water inlet of the stirring pot 102 is connected to the outer tank bottom water outlet 410 at the bottom of the outer tank 414 of the double-layer hard plastic shake pot 400 through a water outlet silicone hose 411. It is noted that the static liquid level of the fluid circulation pump is above the upper water outlet of the stirring pot and below the inner tank water outlet. The fluid circulation pump uses a downward-probing shaker as the motive force to shake the double-layer hard plastic shake pot clockwise (clockwise direction 450), connect the stirring pot, and make the mixed culture fluid circulate in the shaking pot, the stirring pot and the pipeline; the fluid circulation pump is installed on a movable bracket through a downward-probing shaker support shaft.

[0057] In this embodiment, preferably, a stirring shaft 108 is provided in the stirring tank 102, a stirring paddle 120 is installed on the stirring shaft 108, and a motor 110 is installed at the end of the stirring shaft 108. Preferably, any one of the outlet silicone hose 411 and the two return silicone hoses 421 is provided with a hollow fiber microfiltration membrane column 500; the hollow fiber microfiltration membrane column 500 is connected to the stirring tank 102 via a fixing clamp.

[0058] In the present embodiment, preferably, the double-layer hard plastic shaking pot 400 comprises: an outer pot 414, an inner pot 405 and a pot cover 402; an outer pot bottom water outlet 410 is arranged at the bottom center of the outer pot 414; the upper part of the outer pot 414 is in the shape of a conical pot body, and the lower part of the outer pot 414 is in the shape of a barrel pot body; the outer pot 414 is symmetrically provided with two water inlet convex parts 422 at the upper conical pot body position; four positioning columns 425 are arranged at the bottom of the outer pot 414; the outer pot bottom water outlet 410 at the bottom of the outer pot 414 can extend vertically downward from the mounting holes reserved in the bottom plate 340 of the heat-insulating pot body and the bottom plate 218 of the shaking plate; two water return ports 420 are symmetrically arranged at the bottom of the inner pot 405; The upper part of the inner tank 405 is in the shape of a conical tank body, and the lower part of the inner tank 405 is in the shape of a barrel tank body; the inner tank 405 is symmetrically provided with an inner tank water outlet at the position of its upper conical tank body; an inner tank water outlet tangential flow baffle 461 is provided on one side edge of the inner tank water outlet; an inner tank water outlet lower horizontal flow baffle 462 is provided on the bottom side edge of the inner tank water outlet; the two return water ports 420 at the bottom of the inner tank 405 can extend vertically downward from the mounting holes reserved on the insulation tank body bottom plate 340 and the rocking plate bottom plate 218; the tank cover 402 and the flange of the outer tank 414 are sealed and connected by the outer tank body flange sealing rubber ring 423; and a plurality of air filters 403 are provided on the tank cover 402.

[0059] Preferably, the inner tank 405 is inserted into the inner part of the outer tank 414 , and a positioning seat is provided at the bottom of the inner tank 405 , and the positioning seat is inserted and connected with the positioning column 425 at the bottom of the outer tank 414 .

[0060] In this embodiment, preferably, the fluid circulation pump comprises: a movable bracket 600; the movable bracket 600 comprises: a bracket 601, a movable bracket bottom plate 610, and four movable bracket running wheels 620;

[0061] The top of the bracket 601 is provided with a pipe clamp 605, and the pipe clamp 605 is used to pass through the downward-protruding rocking table support shaft 220, and the rocking table body 202 is installed on the downward-protruding rocking table support shaft 220; the lower part of the bracket 601 is provided with a movable bracket bottom plate 610; the bottom of the bracket 601 is provided with four movable bracket wheels 620.

[0062] Preferably, the rocking table body 202 is mounted on a downward rocking table support shaft 220. The rocking table 208 on the top of the rocking table body 202 is mounted on an upper load-bearing plate 210; the four corners of the upper load-bearing plate 210 are respectively mounted on the upper ends of four downward columns 214, and a column fixing frame 216 is installed in the middle of the four downward columns 214; the lower ends of the four downward columns 214 are respectively mounted on a rocking table bottom plate 218; the rocking table bottom plate 218 is installed with a sliding sleeve 320 and a sleeve locking screw 322, and the sleeve locking screw 322 is installed outside the center line of the sliding sleeve 320; the sliding sleeve 320 is slidably installed with an openable and closable heat preservation tank body, and an installation port for the pipeline to extend vertically downward is reserved between the bottom of the heat preservation tank body and the rocking table bottom plate 218, which meets the fluid mechanics requirements of the circulating pump and has better effects than the existing traditional rocking tables. Because the traditional shaking table has a shaking table body (shaking table drive box part) at its lower part, due to structural limitations, there is no space for the pipeline to extend vertically downward in the middle of the shaking table, and the pipeline has to be set in a bend. From the perspective of fluid mechanics, this is obviously not as good as the downward-protruding shaking table in this embodiment.

[0063] In this embodiment, the specific form of the rocking table body 202 is not limited and can refer to the prior art.

[0064] For example, the rocking bed body 202 includes: a rocking bed drive box; a driving motor is installed in the rocking bed drive box; a small pulley is installed on the power output shaft of the driving motor; the small pulley is connected to the large pulley through a belt tensioning connection; the large pulley is installed on the active eccentric shaft; the upper end of the active eccentric shaft is connected to the upper rocking plate; the upper rocking plate is connected to four sets of driven shafts; the upper rocking plate is connected to the rocking plate 208 by bolts, and the rocking plate 208 is installed on the upper bearing plate 210. It can be seen that in the downward rocking bed in this embodiment, the upper bearing plate 210 is fixed on the rocking plate 208, and four downward columns 214 are installed downwardly under the upper bearing plate 210, and the lower ends of the downward columns 214 are equipped with a rocking plate bottom plate 218. A column fixing frame 216 is installed on the upper half of the four columns to ensure the rigidity and stability of the frame; a sliding sleeve 320, a sleeve locking screw 322 and a stopper 312 are installed on the rocker bottom plate 218, and an insulation tank bottom plate 340 is installed on the top of the two sliding sleeves 320, and an insulation tank body that can be divided into two halves is installed thereon. At this point, the equipment is installed.

[0065] In this embodiment, preferably, the openable and closable insulation tank body includes: an insulation tank body bottom plate 340, an insulation tank front half 301 and an insulation tank rear half 300; the insulation tank body bottom plate 340 is installed with the insulation tank front half 301 and the insulation tank rear half 300; the bottom of the insulation tank body bottom plate 340 is provided with a sliding seat 321 for being sleeved on the sliding sleeve 320; the insulation tank front half 301 and the insulation tank rear half 300 are connected on the rotating side through the insulation tank body hinge 330; the insulation tank front half 301 and the insulation tank rear half 300 are provided with locking bolts 350 on the opening and closing side.

[0066] In this embodiment, preferably, the bottom plate 340 of the heat-insulating tank body is provided with a U-shaped installation opening.

[0067] Correspondingly, the rocker bottom plate 218 is also provided with a U-shaped mounting opening.

[0068] At the same time, a space for accommodating the double-layer hard plastic shake pot 400 is formed between the front half 301 of the heat preservation pot and the rear half 300 of the heat preservation pot. The maximum opening and closing angle between the front half 301 of the heat preservation pot and the rear half 300 of the heat preservation pot can be set to 79°.

[0069] In this embodiment, preferably, the rocking plate bottom plate 218 is provided with a stopper 312 on the sliding path of the insulation tank body, and the outer side of the insulation tank body bottom plate 340 has an L-shaped stopper; the stopper 312 is adapted to the L-shaped stopper on the outer side of the insulation tank body bottom plate 340.

[0070] In this embodiment, preferably, four tank positioning pins 303 are provided between the front half 301 and the rear half 300 of the heat preservation tank.

[0071] In this embodiment, during the cell retention process, the cell mixture flows in a single direction in a large circulation loop inside and outside the stirring tank and the double-layer hard plastic shaking tank. The flow rate is stable, without frequency, and the direction and pressure change repeatedly at high speed, resulting in very little cell damage (because animal cells themselves have no cell walls, only a thin cell membrane, which can be broken by very small shear force).

[0072] In this embodiment, during the cell trapping process, the oscillating motion of the mixed liquid in the tank causes the water splashes and water droplets to be raised, which greatly increases the gas-liquid ratio surface area in the shaking tank, thereby greatly increasing the dissolved oxygen level and speed, and also greatly improving the speed of removing harmful gases such as carbon dioxide and ammonia from the mixed liquid, thereby having the functions of increasing oxygen and removing harmful gases. At present, the existing cell trapping devices in the world do not have these two functions.

[0073] In this embodiment, since the volume of the shaking tank can be very large, the diameter of the circulation pipeline can also be very large, and the shaking speed can also be very large, the result is that the flow rate in the large circulation loop can reach a very large value, and the flow rate can also reach a very high value. For example: the volume of the shaking tank is 20 liters, the rotation speed is 80 revolutions per minute, and the flow measurement is 4 times the volume, that is, 80 liters per minute. Moreover, the volume of the shaking tank of the present invention can be enlarged to 200 liters or even 500 liters, which far exceeds the flow rate of existing cell retention devices in the world. In this way, the larger the circulation flow rate, the higher the dissolved oxygen level and the faster the dissolved oxygen speed. The present invention can better meet the needs of large-scale animal cell culture with large volume and high density. Embodiment 3

[0074] Combination Fig.12 As shown, this embodiment 3 provides a macrocirculatory cell retention device based on the above-mentioned embodiments 1 and 2, including: a supernatant extraction and backwashing system and the fluid circulation pump described in embodiment 2. It can be understood that the macrocirculatory cell retention device specifically includes: a downward-probing shaker, a double-layer hard plastic shake tank 400, a stirring tank 102, a movable bracket 600 and a supernatant extraction and backwashing system;

[0075] like Fig.13As shown, in this embodiment, preferably, the supernatant extraction and backwashing system includes: a hollow fiber microfiltration membrane column 500, a peristaltic pump 570, a diaphragm pump assembly 560, an insulation tank 586, a supernatant turnover bag 580, a supernatant storage bag 583 and a supernatant storage tank 585; the upper part of the hollow fiber microfiltration membrane column 500 is provided with a supernatant extraction port 520, and the lower part of the hollow fiber microfiltration membrane column 500 is provided with a supernatant backwashing inlet 510; the peristaltic pump One end of the peristaltic pump hose 572 of 570 is connected to the supernatant extraction port 520, the other end of the peristaltic pump hose 572 of the peristaltic pump 570 is connected to the upper part of the supernatant turnover bag 580, the lower part of the supernatant turnover bag 580 is connected to the diaphragm pump assembly 560; the middle part of the supernatant turnover bag 580 is connected to the supernatant storage bag 583 in the supernatant storage tank 585; the diaphragm pump assembly 560 is connected to the supernatant backwash inlet 510.

[0076] The working process of the supernatant extraction and backwashing system is as follows: the upper end of the hollow fiber microfiltration membrane column 500 is connected to the water outlet at the upper part of the stirring tank 102 through the liquid inlet silicone hose 503, and the cell mixture flows into the hollow fiber microfiltration membrane column 500 from top to bottom and flows out from the lower end, and flows out through the liquid outlet silicone hose 504 connected to the lower part of the stirring tank 102 (the liquid outlet silicone hose 504 is fixed by a silicone tube band 507). The upper part of the hollow fiber microfiltration membrane column 500 has a supernatant extraction port 520, and the lower part has a supernatant backwashing inlet 510; the hollow fiber microfiltration membrane column 500 has a hollow fiber membrane 502. The hollow fiber microfiltration membrane column 500 is fixed to the outer wall of the stirring tank 102 by a fixing clamp 590; the peristaltic pump 570 extracts the supernatant through the peristaltic pump hose 572 and then pumps it into the supernatant turnover bag 580; the supernatant turnover bag 580 has an air filter 582 on the upper part and a supernatant overflow port in the middle. When the supernatant level is higher than the supernatant overflow port, the supernatant will flow from the supernatant inlet 589 into the supernatant storage bag 583 for storage, and the supernatant storage bag 583 has an air filter 587 on the upper part. The lower part of the supernatant turnover bag 580 has a liquid outlet for backwashing. The supernatant turnover bag 580 is entirely installed in the heat-insulating tank body; the supernatant storage bag 593 is specially used to store the supernatant overflowing from the supernatant overflow port of the supernatant turnover bag 580, and it is also in a sealed and sterile state 10.

[0077] In this embodiment, preferably, the diaphragm pump assembly 560 is connected to the supernatant backwash inlet 510 at the lower outer part of the hollow fiber microfiltration membrane column through a silicone hose at the front end, and is connected to the outflow port of the supernatant turnover bag 580 through a silicone hose at the rear end. The diaphragm pump assembly 560 is provided with a front one-way check valve 564 and a rear one-way check valve 562; at the same time, it has a silicone hollow sphere with rebound vacuum capability and an electromagnet assembly that can be pressed and opened reciprocatingly up and down.

[0078] The fluid circulation pump comprises: a double-layer hard plastic shaking pot 400, a stirring pot 102 and the downward-probing shaking table 200 described in the first embodiment.

[0079] In this embodiment, the double-layer hard plastic shake pot 400 is arranged in an insulated tank body that can be opened and closed; the upper water outlet of the stirring pot is connected to the two return water ports 420 at the bottom of the inner tank 405 of the double-layer hard plastic shake pot 400 through two return water silicone hoses 421; the lower water inlet of the stirring pot 102 is connected to the outer tank bottom water outlet 410 at the bottom of the outer tank 414 of the double-layer hard plastic shake pot 400 through a water outlet silicone hose 411. It is noted that the static liquid level of the fluid circulation pump is above the upper water outlet of the stirring pot and below the inner tank water outlet. The fluid circulation pump uses a downward-probing shaker as the motive force to shake the double-layer hard plastic shake pot clockwise (clockwise direction 450), connect the stirring pot, and make the mixed culture fluid circulate in the shaking pot, the stirring pot and the pipeline; the fluid circulation pump is installed on a movable bracket through a downward-probing shaker support shaft.

[0080] In this embodiment, preferably, a stirring shaft 108 is provided in the stirring tank 102, a stirring paddle 120 is installed on the stirring shaft 108, and a motor 110 is installed at the end of the stirring shaft 108. Preferably, any one of the outlet silicone hose 411 and the two return silicone hoses 421 is provided with a hollow fiber microfiltration membrane column 500; the hollow fiber microfiltration membrane column 500 is connected to the stirring tank 102 via a fixing clamp.

[0081] In the present embodiment, preferably, the double-layer hard plastic shaking pot 400 comprises: an outer pot 414, an inner pot 405 and a pot cover 402; an outer pot bottom water outlet 410 is arranged at the bottom center of the outer pot 414; the upper part of the outer pot 414 is in the shape of a conical pot body, and the lower part of the outer pot 414 is in the shape of a barrel pot body; the outer pot 414 is symmetrically provided with two water inlet convex parts 422 at the upper conical pot body position; four positioning columns 425 are arranged at the bottom of the outer pot 414; the outer pot bottom water outlet 410 at the bottom of the outer pot 414 can extend vertically downward from the mounting holes reserved in the bottom plate 340 of the heat-insulating pot body and the bottom plate 218 of the shaking plate; two water return ports 420 are symmetrically arranged at the bottom of the inner pot 405; The upper part of the inner tank 405 is in the shape of a conical tank body, and the lower part of the inner tank 405 is in the shape of a barrel tank body; the inner tank 405 is symmetrically provided with an inner tank water outlet at the position of its upper conical tank body; an inner tank water outlet tangential flow baffle 461 is provided on one side edge of the inner tank water outlet; an inner tank water outlet lower horizontal flow baffle 462 is provided on the bottom side edge of the inner tank water outlet; the two return water ports 420 at the bottom of the inner tank 405 can extend vertically downward from the mounting holes reserved on the insulation tank body bottom plate 340 and the rocking plate bottom plate 218; the tank cover 402 and the flange of the outer tank 414 are sealed and connected by the outer tank body flange sealing rubber ring 423; and a plurality of air filters 403 are provided on the tank cover 402.

[0082] Preferably, the inner tank 405 is inserted into the inner part of the outer tank 414 , and a positioning seat is provided at the bottom of the inner tank 405 , and the positioning seat is inserted and connected with the positioning column 425 at the bottom of the outer tank 414 .

[0083] In this embodiment, preferably, the fluid circulation pump comprises: a movable bracket 600; the movable bracket 600 comprises: a bracket 601, a movable bracket bottom plate 610, and four movable bracket running wheels 620;

[0084] The top of the bracket 601 is provided with a pipe clamp 605, and the pipe clamp 605 is used to pass through the downward-protruding rocking table support shaft 220, and the rocking table body 202 is installed on the downward-protruding rocking table support shaft 220; the lower part of the bracket 601 is provided with a movable bracket bottom plate 610; the bottom of the bracket 601 is provided with four movable bracket wheels 620.

[0085] Preferably, the rocking table body 202 is mounted on a downward rocking table support shaft 220. The rocking table 208 on the top of the rocking table body 202 is mounted on an upper load-bearing plate 210; the four corners of the upper load-bearing plate 210 are respectively mounted on the upper ends of four downward columns 214, and a column fixing frame 216 is installed in the middle of the four downward columns 214; the lower ends of the four downward columns 214 are respectively mounted on a rocking table bottom plate 218; the rocking table bottom plate 218 is installed with a sliding sleeve 320 and a sleeve locking screw 322, and the sleeve locking screw 322 is installed outside the center line of the sliding sleeve 320; the sliding sleeve 320 is slidably installed with an openable and closable heat preservation tank body, and an installation port for the pipeline to extend vertically downward is reserved between the bottom of the heat preservation tank body and the rocking table bottom plate 218, which meets the fluid mechanics requirements of the circulating pump and has better effects than the existing traditional rocking tables. Because the traditional shaking table has a shaking table body (shaking table drive box part) at its lower part, due to structural limitations, there is no space for the pipeline to extend vertically downward in the middle of the shaking table, and the pipeline has to be set in a bend. From the perspective of fluid mechanics, this is obviously not as good as the downward-protruding shaking table in this embodiment.

[0086] In this embodiment, the specific form of the rocking table body 202 is not limited and can refer to the prior art.

[0087] For example, the rocking bed body 202 includes: a rocking bed drive box; a driving motor is installed in the rocking bed drive box; a small pulley is installed on the power output shaft of the driving motor; the small pulley is connected to the large pulley through a belt tensioning connection; the large pulley is installed on the active eccentric shaft; the upper end of the active eccentric shaft is connected to the upper rocking plate; the upper rocking plate is connected to four sets of driven shafts; the upper rocking plate is connected to the rocking plate 208 by bolts, and the rocking plate 208 is installed on the upper bearing plate 210. It can be seen that in the downward rocking bed in this embodiment, the upper bearing plate 210 is fixed on the rocking plate 208, and four downward columns 214 are installed downwardly under the upper bearing plate 210, and the lower ends of the downward columns 214 are equipped with a rocking plate bottom plate 218. A column fixing frame 216 is installed on the upper half of the four columns to ensure the rigidity and stability of the frame; a sliding sleeve 320, a sleeve locking screw 322 and a stopper 312 are installed on the rocker bottom plate 218, and an insulation tank bottom plate 340 is installed on the top of the two sliding sleeves 320, and an insulation tank body that can be divided into two halves is installed thereon. At this point, the equipment is installed.

[0088] In this embodiment, preferably, the openable and closable insulation tank body includes: an insulation tank body bottom plate 340, an insulation tank front half 301 and an insulation tank rear half 300; the insulation tank body bottom plate 340 is installed with the insulation tank front half 301 and the insulation tank rear half 300; the bottom of the insulation tank body bottom plate 340 is provided with a sliding seat 321 for being sleeved on the sliding sleeve 320; the insulation tank front half 301 and the insulation tank rear half 300 are connected on the rotating side through the insulation tank body hinge 330; the insulation tank front half 301 and the insulation tank rear half 300 are provided with locking bolts 350 on the opening and closing side.

[0089] In this embodiment, preferably, the bottom plate 340 of the heat-insulating tank body is provided with a U-shaped installation opening.

[0090] Correspondingly, the rocker bottom plate 218 is also provided with a U-shaped mounting opening.

[0091] At the same time, a space for accommodating the double-layer hard plastic shake pot 400 is formed between the front half 301 of the heat preservation pot and the rear half 300 of the heat preservation pot. The maximum opening and closing angle between the front half 301 of the heat preservation pot and the rear half 300 of the heat preservation pot can be set to 79°.

[0092] In this embodiment, preferably, the rocking plate bottom plate 218 is provided with a stopper 312 on the sliding path of the insulation tank body, and the outer side of the insulation tank body bottom plate 340 has an L-shaped stopper; the stopper 312 is adapted to the L-shaped stopper on the outer side of the insulation tank body bottom plate 340.

[0093] In this embodiment, preferably, four tank positioning pins 303 are provided between the front half 301 and the rear half 300 of the heat preservation tank.

[0094] In summary, in the cell retention process of the present invention, the cell mixture flows in a single direction in a large circulation loop inside and outside the stirring tank and the double-layer hard plastic shaking tank, with a stable flow rate, no frequency, and high-speed repeated changes in direction and pressure, and the cell damage is very small (because animal cells themselves have no cell walls, only a thin cell membrane, and a very small shear force can break the cell membrane).

[0095] When the macrocirculation cell interception device of the present invention extracts the supernatant, the cell concentration of the cell mixture is always the same as the cell concentration of the cell mixture in the stirring tank. Extracting the supernatant does not increase the concentration, and extraction is very easy.

[0096] When the backwashing system of the macrocirculation cell retention device of the present invention flushes the cells blocked on the micropores of the hollow fiber membrane tube, it can be flushed clean every time without any residue. There is no problem of long-term accumulation. The reason is that the backwashing is in the direction of the outer normal of the micropores of the hollow fiber membrane tube, and the pressure is flushed from the outside of the hollow fiber membrane tube to the inside of the tube. In this way, the direction of the impact force is accurate and it is easy to flush out all the cells blocked on the micropores, and flow out of the lower end of the hollow fiber microfiltration membrane column together with the cell mixture flowing from top to bottom in the hollow fiber membrane tube. Moreover, only a small amount of backwashing liquid is used, and the flushing time is extremely short. This is a special advantage of the backwashing system in the present invention.

[0097] In the process of cell retention, the present invention greatly increases the surface area of ​​gas-liquid ratio in the shaking tank due to the oscillating motion of the mixed liquid in the tank, which raises water splashes and water droplets, thereby greatly increasing the dissolved oxygen level and speed, and also greatly improving the speed of removing harmful gases such as carbon dioxide and ammonia in the mixed liquid, and has the functions of increasing oxygen and removing harmful gases. At present, the existing cell retention devices in the world do not have these two functions.

[0098] In the present invention, since the volume of the shaking pot can be greatly increased, the diameter of the circulation pipeline can also be greatly increased, and the shaking speed can also be greatly increased, the result is that the flow rate in the large circulation loop can reach a large amount and the flow rate can also reach a high amount. For example: the volume of the shaking pot is 20 liters, the rotation speed is 80 revolutions per minute, and the flow measurement is 4 times the volume, that is, 80 liters per minute. Moreover, the volume of the shaking pot of the present invention can be enlarged to 200 liters or even 500 liters, which far exceeds the flow rate of existing cell retention devices in the world. In this way, the greater the circulation flow rate, the higher the dissolved oxygen level and the faster the dissolved oxygen speed. The present invention can better meet the needs of large-scale animal cell culture with large volume and high density.

[0099] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A downward-probing shaking table, characterized in that: include: Shaker body (202); The rocking plate (208) at the top of the rocking table body (202) is mounted on an upper load-bearing plate (210); the four corners of the upper load-bearing plate (210) are respectively mounted on the upper ends of four downward-probing columns (214); the middle parts of the four downward-probing columns (214) are mounted with a column fixing frame (216); the lower ends of the four downward-probing columns (214) are respectively mounted on a rocking plate bottom plate (218); the rocking plate bottom plate (218) is mounted with a sliding sleeve (320) and a sleeve The sleeve locking screw (322) is installed outside the center line of the sliding sleeve (320); the sliding sleeve (320) is slidably installed with an openable and closable heat preservation tank body, and a mounting opening for the pipeline to extend vertically downward is reserved between the bottom of the heat preservation tank body and the rocking table bottom plate (218), and the rocking table body is installed on the downward rocking table support shaft; the openable and closable heat preservation tank body includes: a heat preservation tank body bottom plate (340), a heat preservation tank front half body (301 ) and the rear half of the heat preservation tank (300); the heat preservation tank body bottom plate (340) is mounted with the front half of the heat preservation tank (301) and the rear half of the heat preservation tank (300); the bottom of the heat preservation tank body bottom plate (340) is provided with a sliding seat (321) for sleeved on the sliding sleeve (320); the front half of the heat preservation tank (301) and the rear half of the heat preservation tank (300) are connected on the rotating side through the heat preservation tank body hinge (330); the front half of the heat preservation tank (301) and the heat preservation tank The rear half of the tank (300) is provided with a locking bolt (350) on the opening and closing side; the rocking plate bottom plate (218) is provided with a stopper (312) on the sliding path of the heat-insulating tank body, and the outer side of the heat-insulating tank body bottom plate (340) has an L-shaped stopper; the stopper (312) is matched with the L-shaped stopper on the outer side of the heat-insulating tank body bottom plate (340); and four tank positioning pins (303) are provided between the front half of the heat-insulating tank (301) and the rear half of the heat-insulating tank (300).

2. A fluid circulation pump, characterized in that: include: A double-layer hard plastic shaking pot (400), a stirring pot (102), and a downward-probing shaking table as claimed in claim 1; The double-layer hard plastic shake pot (400) is arranged in an openable and closable heat-insulating pot body; The upper water outlet of the stirring tank is connected to two water return ports (420) at the bottom of the inner tank (405) of the double-layer hard plastic shaking tank (400) via two water return silicone hoses (421); the lower water inlet of the stirring tank is connected to the outer tank bottom water outlet (410) at the bottom of the outer tank (414) of the double-layer hard plastic shaking tank (400) via a water outlet silicone hose (411); a stirring shaft (108) is arranged in the stirring tank (102), a stirring paddle (120) is installed on the stirring shaft (108), and a motor (110) is installed at the end of the stirring shaft (108).

3. The fluid circulation pump according to claim 2, characterized in that: The double-layer hard plastic shaking pot (400) comprises: an outer pot (414), an inner pot (405) and a pot cover (402); an outer pot bottom water outlet (410) is arranged at the center of the bottom of the outer pot (414); the upper part of the outer pot (414) is in the shape of a conical pot body, and the lower part of the outer pot (414) is in the shape of a barrel pot body; the outer pot (414) is symmetrically provided with two water inlet convex parts (422) at the position of the upper conical pot body; four positioning columns (425) are arranged at the bottom of the outer pot (414); the outer pot bottom water outlet (410) at the bottom of the outer pot (414) can extend vertically downward from the mounting holes reserved in the bottom plate (340) of the heat-insulating pot body and the bottom plate (218) of the shaking plate; the bottom of the inner pot (405) is symmetrically provided with two water return ports (420); The upper part of the inner tank (405) is in the shape of a conical tank body, and the lower part of the inner tank (405) is in the shape of a cylindrical tank body; the inner tank (405) is symmetrically provided with an inner tank water outlet at the position of the upper conical tank body; an inner tank water outlet tangential flow baffle (461) is provided at one side edge of the inner tank water outlet; and an inner tank water outlet lower horizontal flow baffle (462) is provided at the bottom side edge of the inner tank water outlet; two return water ports (420) at the bottom of the inner tank (405) can extend vertically downward from the mounting holes reserved in the bottom plate (340) of the heat-insulating tank body and the bottom plate (218) of the rocking plate; the tank cover (402) and the flange of the outer tank (414) are sealed and connected via an outer tank body flange sealing rubber ring (423); and a plurality of air filters (403) are provided on the tank cover (402).

4. The fluid circulation pump according to claim 3, characterized in that: The inner tank (405) is inserted into the interior of the outer tank (414), and a positioning seat is provided at the bottom of the inner tank (405), and the positioning seat is inserted and connected to a positioning column (425) at the bottom of the outer tank (414).

5. The fluid circulation pump according to claim 4, characterized in that: Any one of the water outlet silicone hose (411) and the two water return silicone hoses (421) is provided with a hollow fiber microfiltration membrane column (500); the hollow fiber microfiltration membrane column (500) is connected to the stirring tank (102) via a fixing clamp.

6. The fluid circulation pump according to claim 5, characterized in that: include: Removable bracket (600); The movable support (600) comprises: a support (601), a movable support bottom plate (610), and four movable support wheels (620); A pipe clamp (605) is arranged at the top of the bracket (601), and the pipe clamp (605) is used to install a downward-probing rocking table support shaft (220), and the rocking table body (202) is installed on the downward-probing rocking table support shaft (220); a movable bracket bottom plate (610) is arranged at the bottom of the bracket (601); and four movable bracket running wheels (620) are arranged at the bottom of the bracket (601).

7. A macrocirculatory cell retention device, characterized in that: include: A supernatant extraction and backwashing system and a fluid circulation pump according to any one of claims 2 to 6; The supernatant extraction and backwashing system comprises: a hollow fiber microfiltration membrane column (500), a peristaltic pump (570), a diaphragm pump assembly (560), an insulation tank (586), a supernatant turnover bag (580), a supernatant storage bag (583) and a supernatant storage tank (585); the upper portion of the hollow fiber microfiltration membrane column (500) is provided with a supernatant extraction outlet (520), and the lower portion of the hollow fiber microfiltration membrane column (500) is provided with a supernatant backwashing inlet (510); the peristaltic pump (570) is provided with a peristaltic pump (510) and a backwashing inlet (520). One end of the hose (572) is connected to the supernatant extraction port (520), the other end of the peristaltic pump hose (572) of the peristaltic pump (570) is connected to the upper part of the supernatant turnover bag (580), the lower part of the supernatant turnover bag (580) is connected to the diaphragm pump assembly (560); the middle part of the supernatant turnover bag (580) is connected to the supernatant storage bag (583) in the supernatant storage tank (585); the diaphragm pump assembly (560) is connected to the supernatant backwash inlet (510).

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