Complex special enzyme for efficient sea cucumber peeling and preparation method and application thereof

Abstract

The invention discloses a complex special enzyme for efficient sea cucumber peeling and a preparation method and application thereof. The complex special enzyme comprises the following raw materials: papain, bromelain, beta-glucanase, hemicellulase, phytase, exopetidase, an acidity regulating anti-caking agent, trehalose and the balance glucose; the raw materials are proportioned and put into a three-dimensional stirring device for uniform stirring and mixing, to obtain the complex special enzyme for efficient sea cucumber peeling; sea cucumbers are soaked in clear water, the complex special enzyme for efficient sea cucumber peeling is added in the weight ratio, stirring is conducted, the sea cucumbers can be efficiently and rapidly peeled, and the same effect can be achieved when the sea cucumber peel remover is applied to different types of sea cucumbers such as cucumaria cucumaria and stichopus japonicus. According to the invention, all components are synergistic, and complex enzyme hydrolysis is utilized to control the hydrolysis degree of connective tissue protein of the sea cucumbers, so that the sea cucumbers can be peeled and decolored rapidly and efficiently, and are safe, nutritional and healthy; operations of cryogenic freezing, manual peeling, mechanical peeling and the like are not needed by adopting the complex special enzyme, and the complex special enzyme is easy to operate, and has a remarkable peeling effect.

Description

Technical field

[0001] The invention belongs to the field of biological processing hormones and specifically designs a high-efficiency sea cucumber peeling compound special enzyme, a preparation method and an application thereof.

Background technique

[0002] Sea cucumber has high nutritional value and its demand is increasing. The food developed by using its function has become an important direction of deep processing of sea cucumber. However, there are a lot of pigments and sediment in the skin of sea cucumber, which affects the quality of its products, which is not conducive to the deep processing of sea cucumber products. In the pursuit of nutrition How to peel sea cucumbers quickly and well has become a thorny problem in a modern society with all kinds of food, color, aroma and taste.

[0003] Artificial peeling, acid peeling, alkaline peeling, and monomeric enzyme peeling cannot fully meet the market demand due to problems such as poor controllability, low efficie...

Examples

Embodiment 1

[0040] A preparation method for high-efficiency sea cucumber peeling compound special enzyme, comprising the following steps:

[0041]S1: Extraction of papain: collect papaya pulp, mix papaya pulp and water according to the weight ratio of 1:5, wait for the papaya pulp to be fully dissolved and extract for 30 minutes, filter, and the filtrate will be removed by microfiltration bio-organic membrane within 30 minutes Impurities are sterilized until the total number of food-grade colonies is ≤1×10 4 cfu / g, then concentrated to 70% concentration through ultrafiltration biofilm, freeze-dried to obtain papain with a purity of ≥ 98%, crushed to 90% and can pass through an 80-mesh standard sieve in powder form for future use;

[0042] S2: Extract bromelain: mix pineapple juice and water at a weight ratio of 1:5, and pass the mixed solution through microfiltration bio-organic membrane to remove impurities and bacteria within 30 minutes until the total number of food-grade colonies is ≤...

Embodiment 2

[0052] A preparation method for high-efficiency sea cucumber peeling compound special enzyme, comprising the following steps:

[0053] S1: Extraction of papain: collect papaya pulp, mix papaya pulp and water according to the weight ratio of 1:5, wait for the papaya pulp to be fully dissolved and extract for 30 minutes, filter, and the filtrate will be removed by microfiltration bio-organic membrane within 30 minutes Impurities are sterilized until the total number of food-grade colonies is ≤1×10 4 cfu / g, then concentrated to 70% concentration through ultrafiltration biofilm, freeze-dried to obtain papain with a purity of ≥ 98%, crushed to 90% and can pass through an 80-mesh standard sieve in powder form for future use;

[0054] S2: Extract bromelain: mix pineapple juice and water at a weight ratio of 1:5, and pass the mixed solution through microfiltration bio-organic membrane to remove impurities and bacteria within 30 minutes until the total number of food-grade colonies is ...

Embodiment 3

[0064] A preparation method for high-efficiency sea cucumber peeling compound special enzyme, comprising the following steps:

[0065] S1: Extraction of papain: collect papaya pulp, mix papaya pulp and water according to the weight ratio of 1:5, wait for the papaya pulp to be fully dissolved and extract for 30 minutes, filter, and the filtrate will be removed by microfiltration bio-organic membrane within 30 minutes Impurities are sterilized until the total number of food-grade colonies is ≤1×10 4 cfu / g, then concentrated to 70% concentration through ultrafiltration biofilm, freeze-dried to obtain papain with a purity of ≥ 98%, crushed to 90% and can pass through an 80-mesh standard sieve in powder form for future use;

[0066] S2: Extract bromelain: mix pineapple juice and water at a weight ratio of 1:5, and pass the mixed solution through microfiltration bio-organic membrane to remove impurities and bacteria within 30 minutes until the total number of food-grade colonies is ...

Technical field

[0001] The invention belongs to the field of biological processing hormones and specifically designs a high-efficiency sea cucumber peeling compound special enzyme, a preparation method and an application thereof.

Background technique

[0002] Sea cucumber has high nutritional value and its demand is increasing. The food developed by using its function has become an important direction of deep processing of sea cucumber. However, there are a lot of pigments and sediment in the skin of sea cucumber, which affects the quality of its products, which is not conducive to the deep processing of sea cucumber products. In the pursuit of nutrition How to peel sea cucumbers quickly and well has become a thorny problem in a modern society with all kinds of food, color, aroma and taste.

[0003] Artificial peeling, acid peeling, alkaline peeling, and monomeric enzyme peeling cannot fully meet the market demand due to problems such as poor controllability, low efficie...

Examples

Embodiment 1

[0040] A preparation method for high-efficiency sea cucumber peeling compound special enzyme, comprising the following steps:

[0041]S1: Extraction of papain: collect papaya pulp, mix papaya pulp and water according to the weight ratio of 1:5, wait for the papaya pulp to be fully dissolved and extract for 30 minutes, filter, and the filtrate will be removed by microfiltration bio-organic membrane within 30 minutes Impurities are sterilized until the total number of food-grade colonies is ≤1×10 4 cfu / g, then concentrated to 70% concentration through ultrafiltration biofilm, freeze-dried to obtain papain with a purity of ≥ 98%, crushed to 90% and can pass through an 80-mesh standard sieve in powder form for future use;

[0042] S2: Extract bromelain: mix pineapple juice and water at a weight ratio of 1:5, and pass the mixed solution through microfiltration bio-organic membrane to remove impurities and bacteria within 30 minutes until the total number of food-grade colonies is ≤...

Embodiment 2

[0052] A preparation method for high-efficiency sea cucumber peeling compound special enzyme, comprising the following steps:

[0053] S1: Extraction of papain: collect papaya pulp, mix papaya pulp and water according to the weight ratio of 1:5, wait for the papaya pulp to be fully dissolved and extract for 30 minutes, filter, and the filtrate will be removed by microfiltration bio-organic membrane within 30 minutes Impurities are sterilized until the total number of food-grade colonies is ≤1×10 4 cfu / g, then concentrated to 70% concentration through ultrafiltration biofilm, freeze-dried to obtain papain with a purity of ≥ 98%, crushed to 90% and can pass through an 80-mesh standard sieve in powder form for future use;

[0054] S2: Extract bromelain: mix pineapple juice and water at a weight ratio of 1:5, and pass the mixed solution through microfiltration bio-organic membrane to remove impurities and bacteria within 30 minutes until the total number of food-grade colonies is ...

Embodiment 3

[0064] A preparation method for high-efficiency sea cucumber peeling compound special enzyme, comprising the following steps:

[0065] S1: Extraction of papain: collect papaya pulp, mix papaya pulp and water according to the weight ratio of 1:5, wait for the papaya pulp to be fully dissolved and extract for 30 minutes, filter, and the filtrate will be removed by microfiltration bio-organic membrane within 30 minutes Impurities are sterilized until the total number of food-grade colonies is ≤1×10 4 cfu / g, then concentrated to 70% concentration through ultrafiltration biofilm, freeze-dried to obtain papain with a purity of ≥ 98%, crushed to 90% and can pass through an 80-mesh standard sieve in powder form for future use;

[0066] S2: Extract bromelain: mix pineapple juice and water at a weight ratio of 1:5, and pass the mixed solution through microfiltration bio-organic membrane to remove impurities and bacteria within 30 minutes until the total number of food-grade colonies is ...

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