Chemiluminescent Immunoassay Analyzer

Abstract

The present invention provides a chemiluminescent immunoassay instrument, the chemiluminescent immunoassay instrument has a waste liquid extraction port (6) and a cleaning port (7), the chemiluminescent immunoassay instrument comprises a reaction cup (4), an upper light shielding component (10) and a side light shielding component (20), the upper light shielding component (10) comprises a liquid injection cover plate (11), the side light shielding component (20) comprises a reaction cup turntable (22), and a small light shielding cover plate (21), the reaction cup turntable (22) is provided with a turntable through hole in the axial direction (A) for the reaction cup (4) to pass through, the small light shielding cover plate (21) is cylindrical, one end of the small light shielding cover plate (21) in the axial direction (A) is sleeved on the liquid injection cover plate (11), and the other end abuts against the reaction cup turntable (22) and surrounds the turntable through hole. The mechanism is small in size, low in cost, compact in structure, reliable in operation, and can effectively block external light from entering the detection system.

Description

Technical Field

[0001] The invention relates to an immunoanalyzer, in particular to a chemiluminescent immunoanalyzer. Background Art

[0002] The chemiluminescent immunoassay analyzer uses chemiluminescent immunoassay technology to perform qualitative or quantitative detection of analytes in human serum, plasma or other body fluid samples.

[0003] Chemiluminescent immunoassay includes two systems: immunoassay and chemiluminescent analysis. The immunoassay system uses chemiluminescent substances or enzymes as markers, directly marking them on antigens or antibodies, and then reacting with antigens and antibodies to form antigen-antibody immune complexes. The chemiluminescent analysis system adds oxidants or enzyme luminescent substrates after the immune reaction. After the chemiluminescent substance is oxidized by the oxidant, it forms an excited intermediate, which emits photons to release energy to return to a stable ground state. The luminescent intensity can be detected using a luminescent signal measuring instrument (photomultiplier tube). Based on the relationship between the chemiluminescent marker and the luminescent intensity, the content of the analyte can be calculated using a standard curve.

[0004] The working principle of the chemiluminescent immunoassay analyzer is as follows: the sample adding module adds the sample and reagent to the reaction cup. After the sample and reagent in the reaction cup are incubated in the incubation zone, the reaction carrier is adsorbed by the magnetic field and the unbound sample and reagent are removed by washing, leaving only the complex formed by the reaction. Then hydrogen peroxide is added, mixed thoroughly, and moved to the measurement area. The light shielding mechanism raises the light shield to exclude external stray light, then adds sodium hydroxide to the reaction cup, and starts the photomultiplier tube at the same time to capture the photons generated by the reaction. The number of photons (luminescence intensity) is measured by the photomultiplier tube, and the luminescence intensity is converted into the concentration of the analyte through the software.

[0005] Since the reactants of the chemiluminescent reaction emit light by themselves, and the light emission is weak (especially when measuring low-concentration substances), the photomultiplier tube should be used to avoid interference from external light and use a dark room for light protection.

[0006] The darkroom structure of existing chemiluminescence immunoassay analyzers is relatively complex, which not only has a high cost, but also has a high probability of failure.

[0007] Chemiluminescent immunoassay analyzers are usually used in point-of-care testing (POCT) equipment, which is characterized by its small size and compact structure. Therefore, there are waste liquid extraction ports and cleaning ports close to the reading port of the photomultiplier tube, and the apertures of these openings are not less than 6 mm. The presence of these large-diameter through holes allows external light, especially strong light, to enter the interior of the reaction disk through these ports, and through the reflection of the light, pass through the gaps between the components, and finally reach the detection surface of the photomultiplier tube, thereby affecting the accuracy of the test results. Summary of the invention

[0008] The purpose of the present invention is to overcome or at least alleviate the deficiencies of the above-mentioned prior art and to provide a chemiluminescent immunoassay analyzer.

[0009] The present invention provides a chemiluminescent immunoassay analyzer, which has a waste liquid extraction port and a cleaning port, and comprises a reaction cup, an upper light shielding component and a side light shielding component, wherein the upper light shielding component and the side light shielding component can block light from reaching the reaction cup from the waste liquid extraction port and the cleaning port,

[0010] The upper light shielding component includes a liquid injection cover plate arranged above the reaction cup,

[0011] The side light shielding component includes a reaction cup turntable that can rotate around its own central axis, and a light shielding small cover plate arranged between the liquid injection cover plate and the reaction cup turntable.

[0012] The reaction cup turntable is provided with a turntable through hole which penetrates in the axial direction for the reaction cup to pass through. The small light-shielding cover plate is cylindrical. One end of the small light-shielding cover plate in the axial direction is sleeved on the liquid injection cover plate, and the other end abuts against the reaction cup turntable and surrounds the turntable through hole.

[0013] Preferably, two concentric cylindrical structures are formed on one end of the liquid injection cover plate facing the reaction cup turntable, and the small light shielding cover plate is embedded between the two cylindrical structures.

[0014] Preferably, the side light-shielding component also includes an upper cover plate, which is formed with an inner cavity and an upper opening, the inner cavity of the upper cover plate accommodates the reaction cup turntable, the upper opening of the upper cover plate cooperates with the outer peripheral surface of the small light-shielding cover plate, and the liquid injection cover plate is fixed to the upper cover plate.

[0015] Preferably, the chemiluminescent immunoassay analyzer further comprises a lower light shielding component, wherein the lower light shielding component comprises an incubation tray.

[0016] The incubation tray accommodates the portion of the reaction cup extending out of the reaction cup turntable,

[0017] The inner peripheral area of ​​the end surface of the incubation tray facing the reaction cup turntable partially protrudes toward the reaction cup turntable to form an annular first-level step, and the first-level step extends into the inner cavity of the upper cover plate.

[0018] Preferably, the chemiluminescent immunoassay analyzer further includes a photomultiplier tube and a light guide rod, and the lower light shielding component further includes a light guide rod sleeve.

[0019] One end of the light guide rod passes through the side wall of the incubation tray and extends to the reaction cup, and the other end of the light guide rod extends out of the incubation tray and is connected to the photomultiplier tube.

[0020] The light guide rod sleeve wraps the portion of the light guide rod exposed outside the incubation tray.

[0021] Preferably, the lower light shielding component further includes two movable light shielding sheets.

[0022] The incubation tray includes an annular incubation groove for accommodating a portion of the reaction cups.

[0023] The movable light shielding sheet can move along the axial direction to enter or leave the incubation tank. When the movable light shielding sheet enters the incubation tank, the two movable light shielding sheets respectively shield light on both sides of the circumference of the reaction cup.

[0024] Preferably, the thickness of the movable light blocking sheet is 3 mm to 5 mm.

[0025] Preferably, in the radial direction of the incubation tray, the size of the movable light shielding sheet is larger than the size of the incubation tank, and the incubation tray is formed with a groove penetrating in the axial direction to accommodate the movable light shielding sheet.

[0026] Preferably, the movable light shielding sheet is driven by a driving mechanism, and the driving mechanism comprises a lead screw motor, a lead screw and a linear guide rail, the lead screw motor provides a driving force, and the linear guide rail provides guidance for the movable light shielding sheet.

[0027] Preferably, the upper light shielding component further includes a water pipe joint and a liquid injection water pipe, the water pipe joint is connected to the upper part of the liquid injection cover plate, and the liquid injection water pipe sequentially passes through the water pipe joint and the liquid injection cover plate and extends to the top of the reaction cup.

[0028] The chemiluminescent immunoassay analyzer according to the present invention has small size, low cost, compact structure, reliable operation, and can effectively block external light from entering the detection system. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 is a schematic diagram of a chemiluminescent immunoassay analyzer according to one embodiment of the present invention.

[0030] Figure 2 FIG. 1 is a cross-sectional view of a partial structure of a chemiluminescent immunoassay analyzer according to one embodiment of the present invention.

[0031] Figure 3 yes Figure 2 A locally enlarged schematic diagram.

[0032] Figure 4FIG. 4 is a cross-sectional view perpendicular to the axial direction of a partial structure of a chemiluminescent immunoassay analyzer according to one embodiment of the present invention.

[0033] Description of reference numerals:

[0034] 10 upper light shielding component; 11 liquid injection cover plate; 12 water pipe joint; 13 liquid injection water pipe;

[0035] 20 lateral light shielding component; 21 small light shielding cover plate; 22 reaction cup turntable; 23 upper cover plate;

[0036] 30 lower light shielding component; 31 incubation tray; 311 first step; 32 movable light shielding sheet; 33 light guide rod sleeve;

[0037] 4 reaction cups; 5 photomultiplier tubes; 51 light guide rods; 6 waste liquid extraction ports; 7 cleaning ports;

[0038] 80 driving mechanism; 81 lead screw motor; 82 lead screw; 83 linear guide rail. DETAILED DESCRIPTION

[0039] The exemplary embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood that these specific descriptions are only used to teach those skilled in the art how to implement the present invention, and are not intended to exhaust all possible modes of the present invention, nor to limit the scope of the present invention.

[0040] Figure 1 It is an overall schematic diagram of a chemiluminescent immunoassay analyzer (hereinafter also referred to as analyzer) according to an embodiment of the present invention. A waste liquid extraction port 6 and a plurality of cleaning ports 7 are arranged in close proximity to the reading port of the photomultiplier tube 5. The analyzer according to the present invention will prevent external light from entering the gap between the reaction cup 4 and the detection surface of the photomultiplier tube 5 through these ports to affect the accuracy of the test results.

[0041] Figures 2 to 4 It is a partial cross-sectional view of a chemiluminescent immunoassay analyzer according to an embodiment of the present invention in a light-shielding darkroom, wherein the light-shielding darkroom is composed of three parts: an upper light-shielding component 10 , a side light-shielding component 20 , and a lower light-shielding component 30 .

[0042] The upper light shielding component 10 includes components such as a liquid injection cover plate 11 , a water pipe joint 12 , and a liquid injection water pipe 13 .

[0043] The side light shielding component 20 includes a light shielding small cover plate 21 , a reaction cup turntable 22 , an upper cover plate 23 and other components.

[0044] The lower light shielding component 30 includes an incubation tray 31 , a movable light shielding sheet 32 ​​, a light guide rod sleeve 33 and other components.

[0045] All the above parts are made of opaque materials to ensure that no light can penetrate the parts.

[0046] According to the following Figures 2 to 4 The various parts of the darkroom device of the mechanism of the present invention are introduced.

[0047] The liquid injection cover plate 11 is arranged just above the reaction cup 4, and a threaded hole is arranged on the upper part of the liquid injection cover plate 11, and the water pipe joint 12 extends into the threaded hole and is threadedly connected with the liquid injection cover plate 11. The liquid injection cover plate 11 and the water pipe joint 12 are also provided with a through hole for the liquid injection water pipe 13 to pass through, and the liquid injection water pipe 13 passes through the water pipe joint 12 and the liquid injection cover plate 11 from top to bottom in sequence, thereby extending to just above the reaction cup 4.

[0048] The upper light shielding component 10 including the liquid injection cover plate 11 , the water pipe joint 12 and the liquid injection water pipe 13 can shield the external light on the top of the reaction cup 4 .

[0049] The reaction cup turntable 22 can rotate around its central axis. A turntable through hole is provided on the reaction cup turntable 22 and passes through in the axial direction A for the reaction cup 4 to pass through. The turntable through hole is a stepped hole. The large end of the stepped hole is arranged at the top to cooperate with the light-shielding small cover plate 21, and the small end of the stepped hole is arranged at the bottom to cooperate with the reaction cup 4, so that when the reaction cup turntable 22 rotates around its central axis, the reaction cup 4 can be driven to rotate together.

[0050] The light-shielding small cover plate 21 is cylindrical, and one end of the light-shielding small cover plate 21 in the axial direction A is sleeved on the liquid injection cover plate 11, and the other end is against the upper surface of the reaction cup turntable 22 and surrounds the turntable through hole. Preferably, the light-shielding small cover plate 21 relies on its own gravity to lean against the upper surface of the reaction cup turntable 22, so that it can fit with the upper surface of the reaction cup turntable 22 without other gravity mechanisms or clamping mechanisms. The outer peripheral surface of the light-shielding small cover plate 21 also cooperates with the upper cover plate 23 to block the entry of external light.

[0051] The upper cover plate 23 is in a cover shape, including a top surface and side walls and an inner cavity surrounded by the top surface and the side walls. The top surface of the upper cover plate 23 has a plurality of openings for forming a cleaning port, a waste liquid extraction port, a liquid injection port, and the like.

[0052] exist Figures 2 to 3 In the chemiluminescent immunoassay analyzer shown, the opening formed on the upper cover plate 23 is used to cooperate with the light-shielding small cover plate 21 to form a liquid injection port. The top surface of the upper cover plate 23 is also fixedly connected to the liquid injection cover plate 11, and the inner cavity of the upper cover plate 23 accommodates the reaction cup turntable 22, and the incubation tray 31 is also partially accommodated in the accommodating cavity of the upper cover plate 23.

[0053] The small light-shielding cover plate 21 in the side light-shielding component 20 can shield the external light transmitted through the gap between the reaction cup turntable 22 and the upper cover plate 23 .

[0054] The incubation tray 31 is arranged below the reaction cup turntable 22, and an annular incubation groove for accommodating the reaction cup 4 is axially arranged on the incubation tray 31. A through hole cooperating with the light guide rod 51 is horizontally arranged on the side wall of the incubation tray 31. The upper cover plate 23 is partially pressed against the outer peripheral area of ​​the incubation tray 31. The inner peripheral area of ​​the incubation tray 31 partially protrudes toward the reaction cup turntable 22 to form a step 311, so that the incubation tray 31 is partially accommodated in the accommodating cavity of the upper cover plate 23. The above-mentioned cooperation formed by the upper cover plate 23 and the incubation tray 31 also helps to block the external light.

[0055] The incubation groove of the incubation tray 31 is an annular groove, so the incubation groove is open on both sides of the circumference of the reaction cup 4. In the present invention, a movable light shielding sheet 32 ​​is respectively arranged on both sides of the circumference of the part of the reaction cup 4 accommodated in the incubation groove. The movable light shielding sheet 32 ​​is arranged close to the reaction cup 4 in the circumferential direction.

[0056] Reference Figure 4 In the radial direction R of the incubation tray 31, the size of the movable light shielding sheet 32 ​​is larger than the size of the incubation tank, and the movable light shielding sheet 32 ​​can axially enter the incubation tray 31 through the through groove provided on the incubation tray 31. The movable light shielding sheet 32 ​​and the incubation tray 31 together surround the reaction cup 4 therein, so that the movable light shielding sheet 31 can block the light transmitted from the incubation tank.

[0057] Preferably, the thickness of the movable light blocking sheet is 3 mm to 5 mm.

[0058] Preferably, the movable light shielding sheet 31 is driven by a driving mechanism 80, which includes a screw motor 81, a screw 82 and a linear guide 83. The screw motor 81 provides driving force, and the linear guide 83 provides guidance for the movable light shielding sheet 32, so that the movable light shielding sheet 32 ​​can axially enter the incubation tray 31.

[0059] One end of the light guide rod 51 passes through the through hole of the side wall of the incubation tray 31 and extends to the reaction cup 4, and the other end of the light guide rod 51 is connected to the photomultiplier tube 5. The part of the light guide rod 51 exposed outside the side wall of the incubation tray 31 is wrapped by the light guide rod sleeve 33.

[0060] The lower light shielding component 30 composed of the incubation tray 31, the movable light shielding plate 32 and the light guide rod sleeve 33 can shield the external light near the reading port of the photomultiplier tube 4.

[0061] The present invention has at least one of the following advantages:

[0062] (i) The mechanism of the present invention shields the four sides of the reaction cup by means of the upper light shielding component 10, the side light shielding component 20 and the lower light shielding component 30, thereby ensuring that the amount of stray light entering is minimized, thereby improving the accuracy of the test results.

[0063] (ii) The mechanism of the present invention adopts a light-blocking small cover plate 21, which can not only replace the fitting method of the motor moving up and down, but also isolate the interference of upper light including strong light, through precise long-distance fitting in the vertical direction and seamless fitting on the reaction cup turntable 22 by gravity. It has a simple structure, low cost and precise control.

[0064] (iii) The mechanism of the present invention adopts the method of driving the movable light shielding sheet 31 by the bottom motor 81, which increases the minimum reflection path of the light in the thickness direction of the movable light shielding sheet 31, greatly improves the light shielding effect, and completely blocks the external stray light at the bottom of the reaction cup, with significant effect. Since the movable light shielding sheet 31 is thickened, the number of light shielding sheets is reduced from the common four to two, which significantly reduces the size of the component and reduces the manufacturing cost of the component.

[0065] (iv) In the case of severe ambient stray light interference, the mechanism of the present invention uses only one motor 81, which has a simple structure, good effect, low cost, few failures, and high space utilization.

[0066] Of course, the present invention is not limited to the above-mentioned embodiments, and those skilled in the art may make various modifications to the above-mentioned embodiments of the present invention under the guidance of the present invention without departing from the scope of the present invention.

Claims

1. A chemiluminescent immunoassay analyzer, comprising a waste liquid extraction port (6) and a cleaning port (7), characterized in that: The chemiluminescent immunoassay analyzer comprises a reaction cup (4), an upper light shielding component (10) and a side light shielding component (20), wherein the upper light shielding component (10) and the side light shielding component (20) can block light from reaching the reaction cup (4) from the waste liquid extraction port (6) and the cleaning port (7). The upper light shielding component (10) comprises a liquid injection cover plate (11) arranged above the reaction cup (4), The side light shielding component (20) comprises a reaction cup turntable (22) which can rotate around its own central axis, and a light shielding small cover plate (21) arranged between the liquid injection cover plate (11) and the reaction cup turntable (22). The reaction cup turntable (22) is provided with a turntable through hole which penetrates in the axial direction (A) for the reaction cup (4) to pass through, and the light shielding small cover plate (21) is cylindrical, and one end of the light shielding small cover plate (21) in the axial direction (A) is sleeved on the liquid injection cover plate (11), and the other end abuts against the reaction cup turntable (22) and surrounds the turntable through hole.

2. The chemiluminescent immunoassay analyzer according to claim 1, characterized in that: One end of the liquid injection cover plate (11) facing the reaction cup turntable (22) is formed with two concentric cylindrical structures, and the light shielding small cover plate (21) is embedded between the two cylindrical structures.

3. The chemiluminescent immunoassay analyzer according to claim 1, characterized in that: The side light shielding component (20) also includes an upper cover plate (23), the upper cover plate (23) is formed with an inner cavity and an upper opening, the inner cavity of the upper cover plate (23) accommodates the reaction cup turntable (22), the upper opening of the upper cover plate (23) cooperates with the outer peripheral surface of the light shielding small cover plate (21), and the liquid injection cover plate (11) is fixed to the upper cover plate (23).

4. The chemiluminescent immunoassay analyzer according to claim 3, characterized in that: The chemiluminescent immunoassay analyzer further comprises a lower light shielding component (30), wherein the lower light shielding component (30) comprises an incubation tray (31). The incubation tray (31) accommodates the portion of the reaction cup (4) extending out of the reaction cup turntable (22). The inner peripheral area of ​​the end surface of the incubation plate (31) facing the reaction cup turntable (22) partially protrudes toward the reaction cup turntable (22) to form an annular first step (311), and the first step (311) extends into the inner cavity of the upper cover plate (23).

5. The chemiluminescent immunoassay analyzer according to claim 4, characterized in that: The chemiluminescent immunoassay analyzer further comprises a photomultiplier tube (5) and a light guide rod (51), and the lower light shielding component (30) further comprises a light guide rod sleeve (33). One end of the light guide rod (51) passes through the side wall of the incubation tray (31) and extends to the reaction cup (4), and the other end of the light guide rod (51) extends out of the incubation tray (31) and is connected to the photomultiplier tube (5). The light guide rod sleeve (33) wraps the portion of the light guide rod (51) exposed outside the incubation tray (31).

6. The chemiluminescent immunoassay analyzer according to claim 4, characterized in that: The lower light shielding component (30) further comprises two movable light shielding sheets (32). The incubation tray (31) comprises an annular incubation groove for accommodating a portion of the reaction cup (4). The movable light shielding sheet (32) can move along the axial direction (A) to enter or leave the incubation tank. When the movable light shielding sheet (32) enters the incubation tank, the two movable light shielding sheets (32) respectively shield light on both sides of the circumference of the reaction cup (4).

7. The chemiluminescent immunoassay analyzer according to claim 6, characterized in that: The thickness of the movable light blocking sheet (32) is 3 mm to 5 mm.

8. The chemiluminescent immunoassay analyzer according to claim 6, characterized in that: In the radial direction (R) of the incubation tray (31), the size of the movable light shielding plate is larger than the size of the incubation tank, and the incubation tray (31) is formed with a groove penetrating in the axial direction (A) to accommodate the movable light shielding plate (32).

9. The chemiluminescent immunoassay analyzer according to claim 6, characterized in that: The movable light shielding plate (32) is driven by a driving mechanism (80), wherein the driving mechanism (80) comprises a screw motor (81), a screw (82) and a linear guide rail (83), wherein the screw motor (81) provides a driving force, and the linear guide rail (83) provides guidance for the movable light shielding plate (32).

10. The chemiluminescent immunoassay analyzer according to claim 1, characterized in that: The upper light shielding component (10) further comprises a water pipe joint (12) and a liquid injection water pipe (13), wherein the water pipe joint (12) is connected to the upper part of the liquid injection cover plate (11), and the liquid injection water pipe (13) passes through the water pipe joint (12) and the liquid injection cover plate (11) in sequence and extends to the top of the reaction cup (4).

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