A system for preparing samples for mass spectrometry identification of microorganisms

Abstract

The present invention discloses a system for preparing samples for mass spectrometry identification of microorganisms, including a raw material placement module, a spotting module, a mobile module, a control module and a shell. The raw material placement module includes a bottom base, and a culture dish placement area and a target plate placement area are provided on the bottom base. A target plate placement mechanism is provided on the target plate placement area, and the target plate placement mechanism includes a heating film and an inner positioning block. The spotting module includes a bracket, a collection component, a first mobile module and a heating device. The control module includes an electric controller and an identification mechanism, and a protective door that can be opened and closed is provided on the shell. The mobile module drives the bracket and the bottom base to move relative to each other, so that the inoculation needle moves to the target plate to form a colony after taking bacteria from the culture dish. After the spotting is completed, the heating device heats the inoculation needle to automatically disinfect it, and the heating film is also started to heat up the target plate, and the colonies form samples on the target plate. The present invention can complete the preparation of samples without manual operation, reduce pollution in the preparation process, and improve preparation efficiency.

Description

Technical Field

[0001] The invention relates to a system for preparing a microorganism mass spectrometry identification sample in the technical field of biomedical instruments. Background Art

[0002] The main task of the clinical microbiology laboratory is to assist clinicians in diagnosing and identifying various infectious diseases and to guide the timely and rational use of drugs. However, the cultivation of pathogenic microorganisms often takes a long time, which can easily delay the timely treatment of patients. Therefore, the rapid and accurate identification of infectious bacteria is the key to determining whether patients can receive timely treatment.

[0003] Matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology determines the type of microorganism by detecting microorganism-specific fingerprint spectra and then matching and analyzing them with the database. Compared with traditional phenotypic identification and molecular biology techniques, MALDI-TOF MS is fast, accurate, and low-cost, and the database can be continuously updated and improved, which greatly improves the efficiency of clinical microorganism identification, especially microaerobic bacteria, anaerobic bacteria, mycobacteria, fungi and other difficult-to-cultivate microorganisms. Although this technology has been introduced to the clinic for less than 10 years, it is now recognized as a milestone in the rapid identification of microorganisms.

[0004] Pretreatment of microbial specimens is an important part of the inspection process. It is labor-intensive and time-consuming, and operators are exposed to the risk of biological infection. Manual operations are greatly affected by human factors, which directly affects the accuracy of all subsequent tests. Therefore, a fully automatic microbial sample processing system with standard processing capabilities is particularly needed in the market, which can automatically, quickly and safely complete the inoculation of colonies from culture dishes to MALDI-TOF MS target plates and perform lysis and fixation. Summary of the invention

[0005] The object of the present invention is to solve at least one of the technical problems existing in the prior art and to provide a system for preparing microbial mass spectrometry identification samples, which can quickly and automatically prepare mass spectrometry samples.

[0006] According to an embodiment of the present invention, a system for preparing microbial mass spectrometry identification samples is provided, comprising a raw material placement module, the raw material placement module comprising a bottom base, the bottom base being provided with a culture dish placement area and a target plate placement area, the target plate placement area being provided with a target plate placement mechanism, the target plate placement mechanism comprising a heating film and an inner positioning block, the top of the inner positioning block being used to support the target plate, the heating film being installed between the inner positioning block and the bottom base; a spotting module, the spotting module comprising a bracket, a collection component, a first movable module and a heating device, the collection component comprising a mounting substrate and an inoculation needle fixed on the mounting substrate, the inoculation needle being connected to the heating device, the mounting substrate being movably connected to the bracket, the first A moving module is connected to the mounting substrate, and the first moving module is used to drive the inoculation needle to move in the vertical direction; a moving module, the moving module is connected to the base or the bracket, and the moving module is used to drive the relative movement of the bracket and the base; a control module, the control module includes an electric controller and an identification mechanism, the identification mechanism is used to identify the culture dish on the culture dish placement area, the identification mechanism is electrically connected to the electric controller, and the electric controller is electrically connected to the raw material placement module, the spotting module and the moving module respectively; a shell, the shell is provided with an openable and closable protective door, a cavity is formed in the shell, and the raw material placement module, the spotting module, the moving module and the identification mechanism are all arranged in the cavity.

[0007] According to an embodiment of the present invention, further, the shell has an opening, the protective door is installed on the opening, and a door power mechanism is provided in the cavity, the door power mechanism includes a first moving component and a second moving component, the first moving component is installed on the output end of the second moving component, the output end of the first moving component is connected to the protective door, the first moving component is used to change the distance between the protective door and the opening, and the second moving component is used to drive the protective door to move horizontally away from or closer to the opening.

[0008] According to an embodiment of the present invention, further, the spotting module also includes a guiding mechanism, the guiding mechanism includes a slide rail and a sliding member that cooperate with each other, the slide rail is fixed on the bracket, the sliding member is connected to the mounting substrate, and the output end of the first movable module is connected to the sliding member.

[0009] According to an embodiment of the present invention, further, the number of the inoculation needles is more than two, the mounting substrate is movably mounted on the sliding member, the spotting module also includes a switching power member, the switching power member is connected to the mounting substrate, the switching power member is used to drive the mounting substrate to move along a preset direction, and the inoculation needles are arranged at intervals along the preset direction.

[0010] According to an embodiment of the present invention, further, a reagent placement area is provided on the base, and the collection component further comprises at least two injection needles, and the injection needles are arranged at intervals along a preset direction.

[0011] According to an embodiment of the present invention, further, the control module also includes a positioning mechanism, which is electrically connected to the electronic controller, and the positioning mechanism includes a positioning camera and a monitoring component, the positioning camera is used to locate the position of the target plate and the position of the bacterial colony in the culture dish, and the monitoring component is used to locate the position of the inoculation needle and the injection needle.

[0012] According to an embodiment of the present invention, further, two positioning blocks arranged opposite to each other are provided on the culture dish placement area, and a culture dish placement space capable of accommodating the culture dish is formed between the two positioning blocks.

[0013] According to an embodiment of the present invention, further, the target plate placement mechanism also includes a target plate seat, the top of the target plate seat is provided with a first groove for accommodating the target plate, the bottom of the target plate seat is provided with a second groove that can be sleeved on the inner positioning block, and the first groove is connected to the second groove.

[0014] According to an embodiment of the present invention, further, the mobile module includes a second mobile module and a third mobile module, the third mobile module is connected to the output end of the second mobile module, the bottom base is connected to the output end of the third mobile module, and a preset angle is formed between the moving direction of the second mobile module and the moving direction of the third mobile module.

[0015] According to an embodiment of the present invention, further, a sterilization mechanism is provided in the cavity.

[0016] The beneficial effects of the present invention are as follows: the mobile module drives the bracket and the bottom base to move relative to each other, so that after the inoculation needle takes bacteria from the culture dish, it moves to the target plate to form a colony, and after the spotting is completed, the heating device heats the inoculation needle to automatically sterilize, and the heating film is also started to heat the target plate, and the colonies form a sample on the target plate. The present invention does not require manual operation and can be widely used in operations such as colony inoculation and preparation of target plates for microbial mass spectrometry identification, reducing pollution in the preparation process and improving preparation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly describes the drawings required for use in the description of the embodiments. Obviously, the drawings described are only part of the embodiments of the present invention, not all of the embodiments, and those skilled in the art can also obtain other design solutions and drawings based on these drawings without creative work.

[0018] Figure 1is a schematic diagram of the appearance of an embodiment of the present invention;

[0019] Figure 2 is a schematic diagram of a protective door and a door power mechanism in an embodiment of the present invention;

[0020] Figure 3 is a schematic diagram of a raw material placement module in an embodiment of the present invention;

[0021] Figure 4 is a schematic diagram of a mobile module in an embodiment of the present invention;

[0022] Figure 5 is a schematic diagram of a spotting module in an embodiment of the present invention;

[0023] Figure 6 Schematic diagram of a control module in an embodiment of the present invention. DETAILED DESCRIPTION

[0024] This section will describe in detail the specific embodiments of the present invention. The preferred embodiments of the present invention are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the present invention, but it cannot be understood as a limitation on the scope of protection of the present invention.

[0025] In the description of the present invention, it should be understood that descriptions involving orientations, such as up, down, front, back, left, right, etc., and orientations or positional relationships indicated are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0026] In the description of the present invention, "several" means one or more, "more" means more than two, "greater than", "less than", "exceed" etc. are understood as not including the number itself, and "above", "below", "within" etc. are understood as including the number itself. If there is a description of "first" or "second", it is only used for the purpose of distinguishing the technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0027] In the description of the present invention, unless otherwise clearly defined, terms such as setting, installing, connecting, etc. should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in the present invention based on the specific content of the technical solution.

[0028] Reference Figure 1 to Figure 6The system for preparing a microbial mass spectrometry identification sample in an embodiment of the present invention comprises a housing 10, a raw material placement module, a spotting module, a mobile module and a control module. The housing 10 is provided with a protective door 20 that can be opened and closed. A cavity is formed in the housing 10. The raw material placement module, the spotting module and the mobile module are all arranged in the cavity. The raw material placement module comprises a bottom base 30, and a culture dish placement area and a target plate placement area are provided on the bottom base 30. It is easy to understand that the target plate 31 referred to in the present application refers not only to a target plate dedicated to a mass spectrometer, but also to any plate-shaped or non-plate-shaped container that can carry a microbial sample. The mobile module is connected to the raw material placement module or the spotting module, and the mobile module is used to drive the relative movement of the spotting module and the raw material placement module. The control module controls the spotting module to collect bacteria from the culture dish 32 and inoculate it on the target plate 31 to form an identification sample, and finally the protective door 20 is opened to take out the identification sample. The system for preparing microbial mass spectrometry identification samples of the present invention can be used in the preparation of microbial mass spectrometry identification target plates, and can also be used in the field of biochemical experiments such as colony selection and inoculation.

[0029] Reference Figure 2, the housing 10 has an opening, the protective door 20 is installed on the opening, a door power mechanism is arranged in the cavity, the door power mechanism includes a first moving component 22 and a second moving component 21, the first moving component 22 is installed on the output end of the second moving component 21, the output end of the first moving component 22 is connected to the protective door 20, the first moving component 22 is used to change the distance between the protective door 20 and the opening, and the second moving component 21 is used to drive the protective door 20 away from or close to the opening. The distance between the protective door 20 and the opening refers to the distance between the end face of the protective door 20 and the plane where the opening is located, and the moving direction of the second moving component 21 is parallel to the plane where the opening is located. In this embodiment, the first moving component 22 drives the protective door 20 to move along the Y axis, and the second moving component 21 drives the protective door 20 to move along the Z axis. Vertical plates 23 for installing the mechanism in the door hole are respectively arranged on two opposite sides of the opening, and the second moving component 21 is fixed on the vertical plates 23. Optionally, the first moving assembly 22 is a linear slide rail module, a connecting frame is provided on the inner side of the protective door 20, and the protective door 20 is connected to the linear slide rail module through the connecting frame. The second moving assembly 21 includes a synchronous belt and two synchronous wheels, the synchronous belt is sleeved on the two synchronous wheels, and the first moving assembly 22 and the synchronous belt are two sections. The two synchronous wheels are respectively a first synchronous wheel and a second synchronous wheel, the first synchronous wheel is rotatably mounted on a vertical plate 23, and the second synchronous wheel is mounted on the same vertical plate 23 through a tensioning plate. Specifically, the second synchronous wheel is rotatably mounted on the tensioning plate, and the tensioning plate is slidably mounted on the vertical plate 23. By adjusting the position of the tensioning plate, the spacing between the two synchronous wheels can be controlled, thereby tensioning the synchronous belt. The door power mechanism includes a motor, and the wheel center of one of the synchronous wheels of the motor is connected to drive the synchronous belt to rotate. Further, in order to ensure that a guide groove 24 is also provided on the vertical plate 23, the first moving assembly 22 can cooperate with the guide groove 24, so as to ensure that the protective door 20 will not deviate when moving along the Z axis. The protective door 20 is embedded in the opening without occupying any extra space outside the outer shell 10. When the protective door 20 is opened, the first moving component 22 is first started to cause the protective door 20 to move outward, and then the second moving component 21 is started to translate the protective door 20 to expose the opening. Correspondingly, the same applies when the protective door 20 is closed.

[0030] Reference Figure 3The raw material placement module includes a bottom base 30, on which a culture dish placement area and a target plate placement area are provided, and a target plate placement mechanism is provided on the target plate placement area. The target plate placement mechanism includes a heating film and an inner positioning block, the top of the inner positioning block is used to support the target plate 31, and the heating film is installed between the inner positioning block and the bottom base 30, that is, the two end surfaces of the heating film are respectively attached to the inner positioning block and the bottom base 30. The heating film can generate heat, and the heat is then conducted to the target plate 31 through the inner positioning block to achieve heating of the target plate 31. The top of the inner positioning block is a plane, and the top of the inner positioning block can directly contact the target plate 31 to increase the contact area between the inner positioning block and the target plate 31, and improve the heating efficiency, improve the heating effect of the target plate 31, and save energy. It can be understood that a temperature sensor is also installed on the inner positioning block, which is used to detect the temperature of the target plate 31 to prevent the sample itself from being damaged by excessive temperature.

[0031] Further, the target plate placement mechanism also includes a target plate seat 34, the top of the target plate seat 34 is provided with a first groove that can accommodate the target plate 31, the bottom of the target plate seat 34 is provided with a second groove that can be sleeved on the inner positioning block, and the first groove is connected to the second groove. In this embodiment, the target plate seat 34 is a hollow frame structure, the first groove of the target plate seat 34 is connected to the second groove, and there is no interlayer in the middle, so that the target plate 31 can directly contact the inner positioning block. The size of the first groove is designed according to the size of the target plate 31, so that the target plate 31 can be placed in the first groove without moving freely, and the target plate 31 can be quickly and conveniently fixed; the second groove is detachably sleeved on the inner positioning block, so as to achieve fast and stable positioning between the target plate 31 and the inner positioning block. It is easy to understand that the target plate seat 34 has a variety of specifications, which can be adaptively replaced according to the specifications of the target plate 31, thereby improving applicability.

[0032] In some embodiments, two positioning blocks 35 are arranged opposite to each other on the culture dish placement area, and a culture dish placement space capable of accommodating the culture dish 32 is formed between the two positioning blocks 35. Optionally, the inner side of the positioning block 35 is arc-shaped to adapt to the outer peripheral shape of the culture dish 32. Placing the culture dish 32 in the culture dish placement space can prevent the culture dish 32 from moving on the bottom base 30, thereby ensuring the accuracy of subsequent bacteria picking.

[0033] In some embodiments, such as the present embodiment, a reagent placement area is further provided on the base 30, and the reagent placement area is used to place reagents such as culture fluid, and the preparation effect of the microbial sample can be improved by dripping the culture fluid onto the target plate 31. Optionally, a plurality of reagent placement slots 33 are provided on the reagent placement area, and the plurality of reagent placement slots 33 are arranged along the X-axis for easy access.

[0034] Reference Figure 5The spotting module includes a bracket 50, a collection component, a first movable module 51 and a heating device. The collection component includes a mounting substrate 53 and an inoculation needle 54 fixed on the mounting substrate 53. The inoculation needle 54 is connected to the heating device. The mounting substrate 53 is movably connected to the bracket 50. The first movable module 51 is connected to the mounting substrate 53. The first movable module 51 is used to drive the inoculation needle 54 to move in the vertical direction. Since the inoculation needle 54 moves in the vertical direction, the height of the inoculation needle 54 relative to the base 30 will change, so that the bacteria picking action can be achieved. In this process, the movement trajectory of the inoculation needle 54 can be a straight line or a curve. The inoculation needle 54 is connected to the heating device, and the heating device can heat the inoculation needle 54, or the inoculation needle 54 can generate heat by itself through conduction, thereby achieving rapid and automatic sterilization of the inoculation needle 54, which is helpful for entering the next bacteria picking work.

[0035] Furthermore, the spotting module further includes a guide mechanism, which includes a slide rail and a sliding member that cooperate with each other, the slide rail is fixed on the bracket 50, the sliding member is connected to the mounting substrate 53, and the output end of the first movable module 51 is connected to the sliding member. The sliding member always slides along the slide rail, thereby limiting the mounting substrate 53 to move linearly under the drive of the first movable module 51 without deviation.

[0036] It can be understood that the inoculation needle 54 includes a nickel-chromium wire, which is folded in half to form a rod-shaped structure with a total length of about 1 to 2 cm. The needle head is the bending part of the nickel-chromium wire, which is convenient for picking up sample bacteria. The two ends of the nickel-chromium wire are connected to 24V DC. When the power is turned on, the needle head burns red, and the temperature can reach 200 to 300°C, which can inactivate the bacteria remaining on the inoculation needle 54 in a short time. The inoculation needle 54 also includes a ceramic tube, which is sleeved on the outside of the rod-shaped structure, which can play the role of insulation, heat insulation and easy fixation.

[0037] In some embodiments, the number of inoculation needles 54 is more than two, the mounting substrate 53 is movably mounted on the sliding member, and the spotting module further includes a switching power member 52, the switching power member 52 is connected to the mounting substrate 53, and the switching power member 52 is used to drive the mounting substrate 53 to move in a preset direction, and each inoculation needle 54 is arranged at intervals along the preset direction. By driving the mounting substrate 53 to move by the switching power member 52, each inoculation needle 54 can be switched to a working position. In the present application, the working position refers to the position where the needle head of the inoculation needle 54 or the injection needle 55 faces the culture dish 32 or the target plate 31. Since the number of inoculation needles 54 is more than two, the efficiency of bacteria picking can be effectively improved. Taking two inoculation needles 54 as an example, the two inoculation needles 54 are respectively the first inoculation needle 54 and the second inoculation needle 54. When the first inoculation needle 54 is performing bacteria picking, the second inoculation needle 54 can be heated and sterilized; after the first inoculation needle 54 completes bacteria picking, the second inoculation needle 54 is sterilized and cooled to room temperature, and then the second inoculation needle 54 is switched to the working position by switching the power part 52. This cycle is repeated to achieve uninterrupted bacteria picking operation.

[0038] As one optional embodiment, the preset direction is the horizontal direction, the switching power member 52 is a linear module, and the preset direction is parallel to the horizontal direction. Specifically, the preset direction is parallel to the X-axis, so that different inoculation needles 54 correspond to the culture dish 32 or the target plate 31. As another optional embodiment, as in this embodiment, the preset direction is a closed loop direction, the switching power member 52 is a rotating module, and the mounting base plate 53 can rotate on the sliding member to switch the inoculation needle 54 to the working position, effectively saving the installation space.

[0039] In some embodiments, when a reagent placement area is provided on the base 30, the collection assembly further includes at least two injection needles 55, and the injection needles 55 are arranged at intervals along a preset direction. Each injection needle 55 can be switched to a working position by switching the power member 52, so as to contact reagents such as lysate and matrix solution to the target plate 31. It is easy to understand that each injection needle 55 is used to absorb different reagents for injection, so as to better adapt to the sample preparation of more different types of microorganisms.

[0040] Reference Figure 4 , the moving module is connected to the bottom base 30 or the bracket 50, and the moving module is used to drive the relative movement of the bracket 50 and the bottom base 30, so that the inoculation needle 54 can move between the top of the culture dish 32 and the top of the target plate 31, and the injection needle 55 can move between the top of the reagent placement area and the top of the target plate 31, so as to output the bacteria or reagent to the target plate 31 to form a sample. In this embodiment, the moving module is connected to the bottom base 30, which can not only drive the relative movement between the bracket 50 and the bottom base 30, but also drive the bottom base 30 to extend out of the opening, which is more convenient for reagent replenishment or sample placement.

[0041] The mobile module includes a second mobile module 41 and a third mobile module 42, the third mobile module 42 is connected to the output end of the second mobile module 41, the bottom base 30 is connected to the output end of the third mobile module 42, and a preset angle is formed between the moving direction of the second mobile module 41 and the moving direction of the third mobile module 42. Optionally, the preset intersection angle is a right angle. In this embodiment, the moving direction of the second mobile module 41 is parallel to the Y axis, and the moving direction of the third mobile module 42 is parallel to the X axis. When the second mobile module 41 drives the bottom base 30 to move along the Y axis, the collection component can move relatively between the reagent placement area, the culture dish 32 and the target plate 31 to complete the operation of picking bacteria or injecting reagents. When the third movable module 42 drives the bottom base 30 to move along the X-axis, the collection assembly can move in the X-axis direction relative to the bottom base 30, so that the inoculation needle 54 can inoculate multiple colonies on the target plate 31 along the X-axis to increase the number of colonies in the sample; at the same time, since the multiple reagent placement slots 33 in the reagent placement area are also arranged along the X-axis, the injection needle 55 can also absorb different reagents.

[0042] Reference Figure 6 The control module includes an electric controller and an identification mechanism. The identification mechanism is used to identify the culture dish 32 on the culture dish placement area. The identification mechanism is electrically connected to the electric controller, and the electric controller is electrically connected to the raw material placement module, the sample spotting module and the moving module respectively. Optionally, the identification mechanism includes a barcode reading camera 61. The barcode reading camera 61 is arranged at the bottom of the culture dish placement space. A barcode is attached to the outer bottom surface of the culture dish 32. When the culture dish 32 is placed in the culture dish placement space, the camera of the barcode reading camera 61 is aimed at the barcode on the outer bottom surface of the culture dish 32, so as to read the information of the bacterial species in the culture dish 32. The barcode reading camera 61 transmits the information of the culture dish 32 to the electric controller, so that the electric controller can control the relative movement of the collection component and the base, and drip different reagents onto the target plate 31 to fix the microorganisms and prepare samples.

[0043] Furthermore, the control module also includes a positioning mechanism, which is electrically connected to the electric controller, and the positioning mechanism includes a positioning camera 62 and a monitoring component 63. The positioning camera 62 is used to locate the position of the target plate 31 and the position of the bacterial colony in the culture dish 32, so that the inoculation needle 54 can accurately extend into the culture dish 32 to collect the bacterial species and smear the bacterial species on the target plate 31. The monitoring component 63 is used to locate the position of the inoculation needle 54 and the injection needle 55. Specifically, the monitoring component 63 includes a first monitoring camera and a second monitoring camera. The first monitoring camera is used to monitor the position of the injection needle 55, and the second monitoring camera is used to monitor the position of the inoculation needle 54. The monitoring component 63 can feed back the position information of the injection needle 55 and the inoculation needle 54 to the electric controller.

[0044] In some embodiments, a sterilization mechanism is provided in the cavity, and optionally, the sterilization mechanism includes at least one ultraviolet sterilization lamp. After preparing the sample, turning on the ultraviolet sterilization lamp can sterilize the cavity thoroughly to avoid affecting the next sample preparation.

[0045] A specific method of using the system for preparing a microbial mass spectrometry identification sample in this embodiment is as follows:

[0046] S1. Before use, the protective door 20 is closed and loaded, and the ultraviolet sterilization lamp is turned on to sterilize and disinfect the cavity.

[0047] S2. Start the door power mechanism, the protective door 20 moves outward and then translates upward to expose the opening, the moving module drives the bottom base 30 to move in the Y-axis direction to approach or extend out of the opening, and then the formic acid reagent bottle, matrix reagent bottle, cleaning reagent tube and waste liquid reagent bottle are respectively placed in the reagent placement groove 33, the target plate 31 is placed in the first groove of the target plate seat 34, and the culture dish 32 is placed in the culture dish placement space.

[0048] S3. The door power mechanism is started again to close the protective door 20, and the execution program is input to the electronic controller to start the system operation.

[0049] S4. The barcode reading camera 61 reads the barcode at the bottom of the culture dish 32, the positioning camera locates the positions of the target plate 31 and the colonies in the culture dish 32, and the colonies to be selected are selected in turn in the electronic controller.

[0050] S5. The moving module is started, so that the formic acid solution reagent bottle on the bottom base 30 moves to the bottom of the collection component, and the rotating module drives the mounting base 53 to rotate so that an injection needle 55 rotates to the working position, and then the first moving module 51 drives the collection component to move downward, the injection needle 55 absorbs 1 μL of formic acid solution, and then the collection component rises.

[0051] S6. The moving module is started, so that the target plate 31 on the bottom base 30 moves to the bottom of the collection assembly, and the injection needle 55 drips the formic acid solution to the corresponding target point.

[0052] S7. Repeat steps S5 to S6 according to the number of bacteria to be picked.

[0053] S8. The moving module is started, so that the culture dish 32 on the base 30 moves to the bottom of the collection component, and the rotating module drives the mounting base 53 to rotate so that an inoculation needle 54 rotates to the working position, and then the first moving module 51 drives the collection component to move downward, the inoculation needle 54 picks up the colony on the culture dish 32, and then the collection component rises.

[0054] S9. The moving module is started, so that the target plate 31 on the bottom base 30 moves to the bottom of the collection component. The first moving module 51 drives the collection component to move downward so that the inoculation needle 54 can slaughter the microbial colony at the central position of the target point and spread it evenly to form a thin layer, and then the collection component rises.

[0055] S10. Then repeat steps S8 to S9, and at the same time, the corresponding heating device of the inoculation needle 54 used in the previous step is started, and the inoculation needle 54 is heated for sterilization. The two inoculation needles 54 perform the sterilization and sterilization operations alternately.

[0056] S11. After the last bacterial colony to be tested is inoculated onto the target plate 31, wait for 5 minutes, then start the moving module to move the matrix solution bottle on the bottom base 30 to the bottom of the collection component, the rotating module drives the mounting base plate 53 to rotate to rotate another injection needle 55 to the working position, after the injection needle 55 absorbs the matrix solution, the moving module starts again to make the target plate 31 located below the collection component, the injection needle 55 drips the matrix solution onto the target point coated with the bacterial solution, and then the heating film starts to heat to accelerate the volatilization of the matrix;

[0057] S12. After the matrix solution is added to each target point, the moving module is started so that the cleaning reagent tube is located below the collection component. After the injection needle 55 absorbs the cleaning reagent, the moving module is started again so that the waste liquid reagent bottle is located below the collection component, and the injection needle 55 injects the cleaning liquid into the waste liquid reagent bottle.

[0058] S13. After the matrix solution is dripped onto all the target points, the door power mechanism opens the protective door 20, and the moving module pushes the bottom base 30 out of the opening. The target plate 31 can be directly placed in the MALDI-TOF mass spectrometer for microbial identification, the culture dish 32 can be recovered, and the remaining reagents are handled according to regulations.

[0059] S14. The mobile module retracts the base 30 into the cavity, closes the protective door 20, and turns on the ultraviolet sterilization lamp to sterilize and disinfect the cavity.

[0060] The above is a specific description of the preferred implementation mode of the present invention, but the invention is not limited to the embodiments. Those skilled in the art may make various equivalent modifications or substitutions without violating the spirit of the present invention. These equivalent modifications or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A system for preparing samples for mass spectrometry identification of microorganisms, characterized in that: include: A raw material placement module, the raw material placement module comprising a bottom base, the bottom base being provided with a culture dish placement area and a target plate placement area, the target plate placement area being provided with a target plate placement mechanism, the target plate placement mechanism comprising a heating film and an inner positioning block, the top of the inner positioning block being used to support the target plate, the heating film being installed between the inner positioning block and the bottom base; A spotting module, the spotting module comprising a bracket, a collection component, a first movable module and a heating device, the collection component comprising a mounting substrate and an inoculation needle fixed on the mounting substrate, the inoculation needle is connected to the heating device, the mounting substrate is movably connected to the bracket, the first movable module is connected to the mounting substrate, and the first movable module is used to drive the inoculation needle to move in a vertical direction; A moving module, the moving module is connected to the base or the bracket, and the moving module is used to drive the relative movement between the bracket and the base; A control module, the control module includes an electric controller and an identification mechanism, the identification mechanism is used to identify the culture dish on the culture dish placement area, the identification mechanism is electrically connected to the electric controller, and the electric controller is electrically connected to the raw material placement module, the sample spotting module and the moving module respectively; A shell, wherein the shell is provided with an openable and closable protective door, a cavity is formed in the shell, and the raw material placement module, the sample spotting module, the moving module and the identification mechanism are all arranged in the cavity; The spotting module also includes a guiding mechanism, which includes a slide rail and a sliding member that cooperate with each other. The slide rail is fixed on the bracket, and the sliding member is connected to the mounting substrate. The output end of the first movable module is connected to the sliding member. The number of the inoculation needles is more than two, and each of the inoculation needles is arranged at intervals along a preset direction. The mounting substrate is movably mounted on the sliding member. The spotting module also includes a switching power member, which is connected to the mounting substrate. The switching power member is used to drive the mounting substrate to move along a preset direction so that each of the inoculation needles switches to a working position. The inoculation needles include a first inoculation needle and a second inoculation needle. When the first inoculation needle is located at the working position for bacteria picking, the heating device heats and sterilizes the second inoculation needle. After the first inoculation needle completes bacteria picking, the second inoculation needle is sterilized and cooled to room temperature.

2. The system for preparing samples for mass spectrometry identification of microorganisms according to claim 1, characterized in that: The shell has an opening, the protective door is installed on the opening, and a door power mechanism is provided in the cavity. The door power mechanism includes a first moving component and a second moving component. The first moving component is installed on the output end of the second moving component, and the output end of the first moving component is connected to the protective door. The first moving component is used to change the distance between the protective door and the opening, and the second moving component is used to drive the protective door to move horizontally away from or close to the opening.

3. The system for preparing samples for mass spectrometry identification of microorganisms according to claim 1, characterized in that: The bottom base is also provided with a reagent placement area, and the collection component also includes at least two injection needles, and the injection needles are arranged at intervals along a preset direction.

4. The system for preparing samples for mass spectrometry identification of microorganisms according to claim 3, characterized in that: The control module also includes a positioning mechanism, which is electrically connected to the electric controller. The positioning mechanism includes a positioning camera and a monitoring component. The positioning camera is used to locate the position of the target plate and the position of the bacterial colony in the culture dish, and the monitoring component is used to locate the position of the inoculation needle and the injection needle.

5. The system for preparing samples for mass spectrometry identification of microorganisms according to claim 1, characterized in that: The culture dish placement area is provided with two positioning blocks which are arranged opposite to each other, and a culture dish placement space which can accommodate the culture dish is formed between the two positioning blocks.

6. The system for preparing samples for mass spectrometry identification of microorganisms according to claim 1, characterized in that: The target plate placement mechanism also includes a target plate seat, the top of the target plate seat is provided with a first groove for accommodating the target plate, the bottom of the target plate seat is provided with a second groove that can be sleeved on the inner positioning block, and the first groove is connected to the second groove.

7. The system for preparing samples for mass spectrometry identification of microorganisms according to claim 1, characterized in that: The mobile module includes a second mobile module and a third mobile module, the third mobile module is connected to the output end of the second mobile module, the bottom base is connected to the output end of the third mobile module, and a preset angle is formed between the moving direction of the second mobile module and the moving direction of the third mobile module.

8. The system for preparing samples for mass spectrometry identification of microorganisms according to claim 1, characterized in that: A sterilization mechanism is arranged in the cavity.

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