Pseudomonas brassicacearum Y1, culture method and application thereof, and soil conditioner

Abstract

The invention provides a pseudomonas brassicacearum Y1, a culture method and application thereof, and a soil conditioner. The invention discloses pseudomonas brassicacearum Y1 for the first time, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.20127. The pseudomonas brassicacearum Y1 has relatively strong capabilities of dissolving phosphorus, dissolving silicon, dissolving potassium, fixing nitrogen, producing IAA and resisting salt and alkali, can also produce siderophore ACC deaminase, and has oxidase and catalase activity and weak nitrate reductase activity; and the Y1 has a certain antagonistic effect on some phytopathogens. The pseudomonas brassicacearum Y1 is compounded with trichoderma and other components to obtain the soil conditioner, and the soil conditioner can promote the increase of nutrient elements such as available phosphorus, silicon and potassium in soil and promote balanced absorption of nutrients of crops. Tests show that the soil conditioner has an obvious improvement effect on desert soil in China.

Description

technical field

[0001] The invention relates to the technical field of agricultural microorganisms, in particular to Pseudomonas rape Y1, a cultivation method and application thereof, and a soil improver. Background technique

[0002] my country's desertified land area is 1.7212 million km 2 (2014), accounting for 17.93% of the country's land area, with a desertification area of ​​2.6116 million km 2 , accounting for 27.25% of the country's land area. These lands are important cultivated land resources that can be developed. If they can be used, it will not only alleviate the tension of our country's cultivated land resources, but also fully turn various wastes into resources and achieve resource recycling. Restoring the fragile ecological environment plays a key role. How to convert it into arable land requires improvement of these barrier lands, and soil improvement technology also requires the cooperation of improvement substances, which are called soil amendments (soi...

Examples

Embodiment 1

[0048] Example 1 Screening to obtain Pseudomonas rape Y1

[0049] The screening process of Pseudomonas rapeseed Y1 is as follows:

[0050] (1) Retrieve soil samples from the wild walnut tree in Tekes County, Ili Kazak Autonomous Prefecture, Xinjiang, screen Pseudomonas rape Y1 according to the screening medium and conditions of Pseudomonas rape Y1, and isolate and purify, and finally obtain pure strains;

[0051] The screening medium is Ashby medium, the components are as follows: glucose 1%, KH 2 PO 4 0.02%, CaCO 3 0.5%, MgSO 4 ·7H 2 O 0.02%, NaCl 0.02%, CaSO 4 2H 2 O 0.01%, agar 2%, pH 7.0

[0052] Screening conditions: culture at 28°C for 7 days; observe through a microscope to determine whether it is a pure strain, and use the plate streak method to obtain pure strains;

[0053] (2) Prepare the suspension of Pseudomonas rape Y1 strain

[0054] Pick a single colony of the strain and insert it into a simple medium, the components of which are as follows: LB medium...

Embodiment 2

[0121] Example 2 Determination of Pseudomonas rape Y1 inhibiting pathogenic fungi

[0122] Two-point confrontation method: inoculate pathogenic fungi and isolated growth-promoting bacteria on two points 2 cm away from the center on the rye sucrose medium or PDA medium plate [inoculate Phytophthora infestans (Phytophthorainfestans) on rye medium] ; Inoculate Fusarium oxysporum (Fusarium oxysporum) and Sclerotinia (Sclerotinia sclerotiorum) respectively on the PDA medium]. Each treatment was repeated 3 times, and the plate inoculated with pathogenic fungi but not isolated growth-promoting bacteria was used as a control. Culture in dark at 18°C ​​(Phytophthora infestans) or 25°C (Fusarium oxysporum, Sclerotinia sclerotiorum), observe the antibacterial effect after 7 days and calculate the antibacterial rate. Bacterial inhibition rate (%)=(control colony radius-treated colony radius) / control colony radius×100.

[0123] Result: if figure 1 Shown, in the inhibition test of potato...

Embodiment 3

[0128] The culture method of Pseudomonas brassicacearum Y1 (Pseudomonas brassicacearum Y1), comprises the steps:

[0129] (1) Pseudomonas rape Y1 was inoculated into a slant culture medium, activated and cultivated at a temperature of 25-30° C. for 10-16 hours to obtain an activated strain.

[0130] The composition of the slant medium is as follows: tryptone 1%, yeast extract 0.5%, NaCl 1%, agar 2%, pH 7.0.

[0131] (2) Inoculate the activated bacterial strain prepared in step (1) in a primary seed medium, and cultivate it for 10 to 16 hours at a temperature of 25 to 30° C. and a rotating speed of 160 to 240 r / min to obtain a seed solution;

[0132] Primary seed medium: starch starch 0.05%, glucose 0.05%, yeast extract 0.05%, peptone 0.05%, (NH 4 ) 2 SO 4 0.05%, K 2 HPO 4 0.03%, MgSO 4 ·7H 2 O 0.005%, pH 7.0.

[0133] (3) inoculate the seed solution prepared in step (2) in the secondary seed medium, the inoculum amount is 8-12% (volume percentage), and cultivate under ...

technical field

[0001] The invention relates to the technical field of agricultural microorganisms, in particular to Pseudomonas rape Y1, a cultivation method and application thereof, and a soil improver. Background technique

[0002] my country's desertified land area is 1.7212 million km 2 (2014), accounting for 17.93% of the country's land area, with a desertification area of ​​2.6116 million km 2 , accounting for 27.25% of the country's land area. These lands are important cultivated land resources that can be developed. If they can be used, it will not only alleviate the tension of our country's cultivated land resources, but also fully turn various wastes into resources and achieve resource recycling. Restoring the fragile ecological environment plays a key role. How to convert it into arable land requires improvement of these barrier lands, and soil improvement technology also requires the cooperation of improvement substances, which are called soil amendments (soi...

Examples

Embodiment 1

[0048] Example 1 Screening to obtain Pseudomonas rape Y1

[0049] The screening process of Pseudomonas rapeseed Y1 is as follows:

[0050] (1) Retrieve soil samples from the wild walnut tree in Tekes County, Ili Kazak Autonomous Prefecture, Xinjiang, screen Pseudomonas rape Y1 according to the screening medium and conditions of Pseudomonas rape Y1, and isolate and purify, and finally obtain pure strains;

[0051] The screening medium is Ashby medium, the components are as follows: glucose 1%, KH 2 PO 4 0.02%, CaCO 3 0.5%, MgSO 4 ·7H 2 O 0.02%, NaCl 0.02%, CaSO 4 2H 2 O 0.01%, agar 2%, pH 7.0

[0052] Screening conditions: culture at 28°C for 7 days; observe through a microscope to determine whether it is a pure strain, and use the plate streak method to obtain pure strains;

[0053] (2) Prepare the suspension of Pseudomonas rape Y1 strain

[0054] Pick a single colony of the strain and insert it into a simple medium, the components of which are as follows: LB medium...

Embodiment 2

[0121] Example 2 Determination of Pseudomonas rape Y1 inhibiting pathogenic fungi

[0122] Two-point confrontation method: inoculate pathogenic fungi and isolated growth-promoting bacteria on two points 2 cm away from the center on the rye sucrose medium or PDA medium plate [inoculate Phytophthora infestans (Phytophthorainfestans) on rye medium] ; Inoculate Fusarium oxysporum (Fusarium oxysporum) and Sclerotinia (Sclerotinia sclerotiorum) respectively on the PDA medium]. Each treatment was repeated 3 times, and the plate inoculated with pathogenic fungi but not isolated growth-promoting bacteria was used as a control. Culture in dark at 18°C ​​(Phytophthora infestans) or 25°C (Fusarium oxysporum, Sclerotinia sclerotiorum), observe the antibacterial effect after 7 days and calculate the antibacterial rate. Bacterial inhibition rate (%)=(control colony radius-treated colony radius) / control colony radius×100.

[0123] Result: if figure 1 Shown, in the inhibition test of potato...

Embodiment 3

[0128] The culture method of Pseudomonas brassicacearum Y1 (Pseudomonas brassicacearum Y1), comprises the steps:

[0129] (1) Pseudomonas rape Y1 was inoculated into a slant culture medium, activated and cultivated at a temperature of 25-30° C. for 10-16 hours to obtain an activated strain.

[0130] The composition of the slant medium is as follows: tryptone 1%, yeast extract 0.5%, NaCl 1%, agar 2%, pH 7.0.

[0131] (2) Inoculate the activated bacterial strain prepared in step (1) in a primary seed medium, and cultivate it for 10 to 16 hours at a temperature of 25 to 30° C. and a rotating speed of 160 to 240 r / min to obtain a seed solution;

[0132] Primary seed medium: starch starch 0.05%, glucose 0.05%, yeast extract 0.05%, peptone 0.05%, (NH 4 ) 2 SO 4 0.05%, K 2 HPO 4 0.03%, MgSO 4 ·7H 2 O 0.005%, pH 7.0.

[0133] (3) inoculate the seed solution prepared in step (2) in the secondary seed medium, the inoculum amount is 8-12% (volume percentage), and cultivate under ...

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