Indoor breeding method for Microplitis prodeniae Rao and Kurian

Abstract

The invention belongs to the technical field of natural enemy insect breeding, and particularly relates to an indoor breeding method for Microplitis prodeniae Rao and Kurian. The method comprises the following steps: (1) sowing rice seeds and raising seedlings for later use; (2) inoculating larvae of spodoptera frugiperda or prodenia litura on the bred rice seedlings; (3) putting the rice seedlings together with larvae of spodoptera litura or prodenia litura into an insect breeding cage, and culturing indoors; (4) inoculating male and female Microplitis prodeniae Rao and Kurian to enable the Microplitis prodeniae Rao and Kurian to parasitize; (5) removing the Microplitis prodeniae Rao and Kurian; (6) feeding the parasitized larvae in the step (4) until the parasitic wasp mature larvae get out of the wall of the host body, and conducting spinning, cocooning and pupation; (7) picking out cocoons of the Microplitis prodeniae Rao and Kurian, putting the cocoons into a finger-shaped tube, and waiting for eclosion of the cocoons of the Microplitis prodeniae Rao and Kurian; and (8) repeating the steps (1)-(7) to carry out subculture breeding on the Microplitis prodeniae Rao and Kurian. According to the method, the indoor artificial propagation of the Microplitis prodeniae Rao and Kurian is realized.

Description

technical field

[0001] The invention belongs to the technical field of natural enemy insect breeding, in particular to an indoor breeding method of Spodoptera litura. Background technique

[0002] Spodoptera lituraFabricius, Spodopterafrugiperda (Smith), Spodoptera exigua Hübner and Spodoptera mauritia (Boisduval) all belong to Noctuidae of the order Lepidoptera. It is a vicious agricultural pest distributed all over the world. It has the characteristics of wide distribution, miscellaneous food, strong fecundity, and great destructiveness. The larvae eat plants, which can cause holes or nicks in plant leaves, and in severe cases, cause plant death or even complete harvest, which is harmful to agricultural production. It has caused huge economic losses and serious social impacts. At present, chemical control is still the main method for controlling noctuid pests, but problems such as pesticide residues, ecological environment pollution, and increased pest resistance caused b...

Examples

Embodiment 1

[0032] An indoor rearing method of Spodoptera litura side ditch wasp, comprising the following steps:

[0033] (1) the rice seed is soaked in water for 3h to promote germination, and after germination, use the seedling-raising square cup of upper diameter 7cm, bottom diameter 7cm, height 8cm to sow seedlings, when the rice seedlings grow to 12cm, standby;

[0034] (2) inserting 90 Spodoptera frugiperda or Spodoptera litura larvae from the end of the first instar to the second instar on the bred rice seedlings;

[0035] (3) Put the rice seedlings together with the larvae of Spodoptera litura in a cage with a size of 30cm×30cm×30cm, and place the cage at a temperature of 27±2°C and a photoperiod of L:D=14:10 , indoor culture with a relative humidity of 70±5%;

[0036] (4) Put 5 pairs of mated adult Spodoptera litura side ditch bees into the insect cage, and put honey water with a mass concentration of 10% in the insect cage at the same time, and dip it with absorbent cotton. P...

Embodiment 2

[0042] An indoor rearing method of Spodoptera litura side ditch wasp, comprising the following steps:

[0043] (1) the rice seed is soaked in water for 4h to promote germination, and after germination, use the seedling-raising square cup of upper diameter 7cm, bottom diameter 7cm, height 8cm to sow seedlings, when the rice seedlings grow to 10cm, standby;

[0044] (2) insert 100 Spodoptera frugiperda larvae from the end of the first instar to the second instar on the bred rice seedlings;

[0045] (3) Put the rice seedlings together with Spodoptera frugiperda larvae into an insect cage with a size of 30cm×30cm×30cm, and place the insect cage at a temperature of 27±2°C, and the photoperiod is L:D=14: 10. Indoor culture with a relative humidity of 70±5%;

[0046] (4) Put 5 pairs of mated adult Spodoptera litura side ditch bees adult bees after eclosion in the insect cage, put honey water with a mass concentration of 10% in the insect cage at the same time, and dip it with absorb...

Embodiment 3

[0052] A kind of indoor rearing method of Spodoptera litura wasp, comprising the following steps:

[0053] (1) rice seeds are soaked in water for 5 hours to accelerate germination, and after germination, use a seedling-raising square cup with an upper diameter of 7cm, a bottom diameter of 7cm, and a height of 8cm to sow and raise seedlings, and when the rice seedlings grow to 8cm, it is for subsequent use;

[0054] (2) Insert 110 Spodoptera frugiperda larvae from the end of the 1st instar to the 2nd instar on the rice seedlings that have been bred;

[0055] (3) put the rice seedlings together with the Spodoptera frugiperda larvae into the insect cages whose specifications are 30cm×30cm×30cm, and place the insect cages at a temperature of 27±2°C, with a photoperiod of L:D=14: 10. Indoor cultivation with a relative humidity of 70±5%;

[0056] (4) Put 5 pairs of mated adults of Spodoptera litura after mating into the pitcher cage, and put into the pitcher cage at the same time t...

technical field

[0001] The invention belongs to the technical field of natural enemy insect breeding, in particular to an indoor breeding method of Spodoptera litura. Background technique

[0002] Spodoptera lituraFabricius, Spodopterafrugiperda (Smith), Spodoptera exigua Hübner and Spodoptera mauritia (Boisduval) all belong to Noctuidae of the order Lepidoptera. It is a vicious agricultural pest distributed all over the world. It has the characteristics of wide distribution, miscellaneous food, strong fecundity, and great destructiveness. The larvae eat plants, which can cause holes or nicks in plant leaves, and in severe cases, cause plant death or even complete harvest, which is harmful to agricultural production. It has caused huge economic losses and serious social impacts. At present, chemical control is still the main method for controlling noctuid pests, but problems such as pesticide residues, ecological environment pollution, and increased pest resistance caused b...

Examples

Embodiment 1

[0032] An indoor rearing method of Spodoptera litura side ditch wasp, comprising the following steps:

[0033] (1) the rice seed is soaked in water for 3h to promote germination, and after germination, use the seedling-raising square cup of upper diameter 7cm, bottom diameter 7cm, height 8cm to sow seedlings, when the rice seedlings grow to 12cm, standby;

[0034] (2) inserting 90 Spodoptera frugiperda or Spodoptera litura larvae from the end of the first instar to the second instar on the bred rice seedlings;

[0035] (3) Put the rice seedlings together with the larvae of Spodoptera litura in a cage with a size of 30cm×30cm×30cm, and place the cage at a temperature of 27±2°C and a photoperiod of L:D=14:10 , indoor culture with a relative humidity of 70±5%;

[0036] (4) Put 5 pairs of mated adult Spodoptera litura side ditch bees into the insect cage, and put honey water with a mass concentration of 10% in the insect cage at the same time, and dip it with absorbent cotton. P...

Embodiment 2

[0042] An indoor rearing method of Spodoptera litura side ditch wasp, comprising the following steps:

[0043] (1) the rice seed is soaked in water for 4h to promote germination, and after germination, use the seedling-raising square cup of upper diameter 7cm, bottom diameter 7cm, height 8cm to sow seedlings, when the rice seedlings grow to 10cm, standby;

[0044] (2) insert 100 Spodoptera frugiperda larvae from the end of the first instar to the second instar on the bred rice seedlings;

[0045] (3) Put the rice seedlings together with Spodoptera frugiperda larvae into an insect cage with a size of 30cm×30cm×30cm, and place the insect cage at a temperature of 27±2°C, and the photoperiod is L:D=14: 10. Indoor culture with a relative humidity of 70±5%;

[0046] (4) Put 5 pairs of mated adult Spodoptera litura side ditch bees adult bees after eclosion in the insect cage, put honey water with a mass concentration of 10% in the insect cage at the same time, and dip it with absorb...

Embodiment 3

[0052] A kind of indoor rearing method of Spodoptera litura wasp, comprising the following steps:

[0053] (1) rice seeds are soaked in water for 5 hours to accelerate germination, and after germination, use a seedling-raising square cup with an upper diameter of 7cm, a bottom diameter of 7cm, and a height of 8cm to sow and raise seedlings, and when the rice seedlings grow to 8cm, it is for subsequent use;

[0054] (2) Insert 110 Spodoptera frugiperda larvae from the end of the 1st instar to the 2nd instar on the rice seedlings that have been bred;

[0055] (3) put the rice seedlings together with the Spodoptera frugiperda larvae into the insect cages whose specifications are 30cm×30cm×30cm, and place the insect cages at a temperature of 27±2°C, with a photoperiod of L:D=14: 10. Indoor cultivation with a relative humidity of 70±5%;

[0056] (4) Put 5 pairs of mated adults of Spodoptera litura after mating into the pitcher cage, and put into the pitcher cage at the same time t...

Images