45results about How to "Increase pupation rate" patented technology

The invention relates to an artificial feeding method of tested insect, especially relates to an artificial feeding method of tested chive maggot, wherein the Chinese chive feedstuff is replaced by the artificial synthetic feedstuff, thus the survival rate, pupation rate, emergence rate, single-female fecundity are greatly increased compared with the prior tested chive maggot breeding method, therefore the good condition is provided for regular supply of tested chive maggot.

The invention provides a semi-artificial feed for Spodoptera frugiperda and a preparation method of the semi-artificial feed and a method for feeding the Spodoptera frugiperda. The semi-artificial feed comprises soybean meal (7.00%-9.00%), yeast meal (3.00%-4.50%), corn meal (7.50%-9.50%), corn oil (0.20%-0.30%), ascorbic acid (0.20%-0.30%), sorbic acid (0.10%-0.20%), methyl parahydroxybenzoate (0.10%-0.20%), cholesterol (0.060%-0.065%), agar (1.00%-1.50%), and water (75.00%-80.00%), base on weight percentage. The feed provided by the invention has low cost, wide source of feed raw materials,and simple and convenient preparation, is suitable for growth, development and reproduction of the Spodoptera frugiperda, can be used for group feeding, and thus can realize long-term successive large-scale feeding of the Spodoptera frugiperda (large-scale feeding with successive generations). Moreover, the preparation method of the semi-artificial feed for Spodoptera frugiperda is provided.

The invention relates to an artificial large-scale rearing method of tested insects, in particular to an artificial mass rearing method of tested Chinese chive maggot. Potatoes replace existing Chinese chive, green Chinese onion and artificial feed, and a fine sand moisturizing method replaces an agar moisturizing method and an artificial water adding moisturizing method. Compared with an existing tested Chinese chive maggot rearing method, the survival rate, the pupation rate, the emergence rate and the egg number per female of the artificial rearing method are equal to those of the existing tested Chinese chive maggot rearing method, but cost is saved, artificial operation steps are decreased, and favorable conditions are provided for normal supply of the tested Chinese chive maggot.

The invention discloses a method for feeding Chilo sacchariphagus Bjojer or Chilo infuscatellus Snellen indoors. The method comprises the following steps: S1, egg mass disinfection; S2, larva feeding: putting small insect feed, and taking out egg shells and filter paper on the second-third day; and adding big insect feed on the 9th-12th day, using test tubes for single-head feeding on the 18th-22th day, placing the large insect feed again, and adding folded filter paper on the 23rd-26th day for pupation, wherein the small insect feed and the large insect feed comprise agar powder, fresh sweet corn kernels, sugarcane stem powder, casein, whole egg powder, yeast powder, cane sugar, cholesterol, sodium chloride, ascorbic acid, methylparaben, sorbic acid, multivitamin tablets, 40%v / v formaldehyde and distilled water. The formula components are prepared according to different proportions. S3, pupa treatment; S4, adult pairing for egg laying; and S5, circularly feeding. The feeding method is beneficial to the growth requirements of larvae and the requirements of pupation, eclosion, oviposition and other characteristics, can better meet the requirements of the larvae for nutrition, and is beneficial to the growth and development of the larvae.

The invention relates to a device suitable for propagation of tephritidae-type insects and a propagation method of the device. The device comprises a cultivation chamber, a pupating chamber and a ventilation lid; the cultivation chamber and the pupating chamber are both utensils with upper ends opened; the cultivation chamber is formed in the pupating chamber, and the ventilation lid is arranged at the top of the pupating chamber; a dry sand layer is laid at the inner bottom of the pupating chamber, the cultivation chamber is formed in the portion above the dry sand layer, the ventilation lidis in fastened or threaded connection with the pupating chamber, and a ventilation gauze net is arranged in the middle of the ventilation lid; the cultivation chamber is internally provided with an artificial feed layer and a natural feed layer, the artificial feed layer is arranged at the inner bottom of the cultivation chamber, the natural feed layer is arranged on the artificial feed layer, andmoisture-retention gauze is laid on the upper portion of the natural feed layer. The device has the advantages that the device is easy to manufacture, practical and reusable; by means of the method,the survival rate and the pupating rate of tephritidae-type larvae are high and reach 90% or above; by means of the device, the contamination of other indoor and outdoor insects, particularly fruit flies can be avoided, and purification of obtained tephritidae pupae and imagoes is guaranteed.

The invention discloses a method for artificially feeding drosophila suzukii matsumura, wherein adults which are feathered in the same day are selected to put into a self-made adult feeding cage, 5.5g artificial feed is stored in a culture dish A, then the culture dish A is arranged in a culture dish B which is paved with a sterilization filter paper, sterile water is injected on the sterilization filter paper which is arranged in the culture dish B, the culture dish B which is matched with the culture dish A is put into the self-made adult feeding cage, and is put into a phytotron to observe and count number of egg grains, the culture dish A is put into a glass beaker, and is sealed through a Para film and then is put into the phytotron to feed, the growth, the development and the feeding behavior of feed insects are observed in a stereomicroscope, are observed until larva are aged in fixed time every day, the adults which are feathered in the same day are conjugated according to the female-male ratio of 1:1, and the conjugated adults are respectively put into the adult feeding cage to feed. The method for artificially feeding the drosophila suzukii matsumura is simple, convenient in operation and easy to feed targeted insects.

The invention relates to a Chinese chive maggot feeding method. According to the method, existing Chinese chive, artificial feed and potatoes are replaced with carrots and agar moisture retention and fine sand moisture retention are replaced by utilizing garden soil. Compared with an existing test Chinese chive maggot feeding method, the feeding method provided by the invention has the advantages that the survival rate, the pupation rate and the eclosion rate are improved; and the egg number per female is equivalent. The cost is saved and manual operation steps are further reduced so that the Chinese chive maggot feeding method is suitable for feeding Chinese chive maggots.

The invention discloses a barley pest breeding method. The method comprises the following steps: preparation of feed, preparation of a breeding box, preparation of an incubator, feed casting, egg collection and hatching as well as feeding management. When barley pests are bred with the method, mutual interference and killing under the condition of social feeding can be reduced effectively; through strict control of feed and breeding density, the percentage of pupation of the barley pests is increased while the nutrients required by growth of the barley pests is guaranteed, the economic benefit is increased, and the excellent barley pest breeding method has the advantages of low labor intensity, high percentage of pupation, low cost and the like.

The invention discloses a feed for raising chilo venosatus walkers and a preparation method of the feed. The feed is prepared from the following raw materials in parts by weight: 750-770 parts of water, 36-40 parts of yeast extract powder, 28-32 parts of casein, 13-17 parts of agar, 0.4-0.5 part of potassium hydroxide, 3.5-4.0 parts of ascorbic acid, 0.25-0.35 part of multivitamin and minerals tablets, 13-17 parts of cane sugar, 6-8 parts of wheat bran, 8.5-11.5 parts of maize flour, 113-118 parts of sugarcane stem powder and 0-4.4 parts of a preservative. The preparation method comprises the step of uniformly mixing all the raw materials. The feed prepared by the preparation method disclosed by the invention is suitable for feeding the chilo venosatus walkers, the developmental rate, the survival rate, the pupal weight and the pupation rate of a larva can be greatly increased, the developmental physiological status of an insect source are basically consistent, and requirements for nutrient substances of the development of a whole generation or development of generations of the chilo venosatus walkers can also be met.

The invention discloses artificial synthesized feed for proxenuslepigone moschler and a preparation method thereof. The feed adopts wheat bran and mushroom powder as main components and comprises whole meal flour, peptone, and other auxiliary materials. The proxenuslepigone moschler which is fed by the feed in a certain ratio has significantly improved survival rate, pupation rate, eclosion rate and single-female egg laying amount. The feed cannot be influenced by seasons and is free from pesticide or other residual components. In addition, the feed is prepared by a microwave oven with simple operation, the preparation amount can be controlled and the feed can be prepared when needed, thereby improving the feed utilization rate and solving a problem that too much feed is prepared for one time and needs to be stored in a refrigerator.

The invention relates to a large-scale artificial feeding method for tested insects, in particular to a large-scale artificial feeding method for tested bradysia odoriphaga. Conventional leeks, green Chinese onions and artificial feeds are replaced with peanuts, and agar moisture preservation and manual water addition moisture preservation methods are replaced with fine sand. Compared with the conventional feeding method for tested bradysia odoriphaga, the method has the advantages that equivalent survival rate, pupation rate, eclosion rate and egg number per female are achieved, the cost is reduced, manual operation steps are reduced, and favorable conditions are created for the normal supply of the tested bradysia odoriphaga.

The invention discloses a method for reproducing stegobium paniceum (L.) by utilizing semi-artificial feed, belongs to an insect reproduction method, and aims to provide a stegobium paniceum (L.) reproduction method which is uniform in insect growth and development and good in physiological status consistency. The method comprises the steps of egg collection, egg hatching and larvae rearing. Specifically, the method comprises the following steps: putting adults into a culture dish loaded with feed, sealing an opening of the culture dish with a preservative film with vent holes, and then putting the culture dish into a constant-temperature environment; collecting eggs, putting the eggs between two layers of preservative films, and then hatching the eggs in the constant-temperature environment; and after the eggs are hatched into larvae, putting the larvae into a new culture dish loaded with feed, forming a plurality of small holes in the surface of the feed by pricking, sealing an opening of the culture dish with a preservative film with vent holes, and then performing culture in the constant-temperature environment until the larvae grow into adults. The method is simple to operate; the egg hatching rate, the survival rate, the pupation rate and the like are all remarkably increased; and feed raw materials are easily available. The method is the stegobium paniceum (L.) reproduction method.

The present invention discloses an artificial compound feed for tenebrio molitor. The feed is characterized by comprising the following components in mass percentages: 70%-74% of wheat bran, 6%-8% of corn flour, 2%-4% of soybean powder, 4%-6% of milk, 2%-6% of white sugar, 0.3%-0.7% of compound vitamin, 2.2%-2.5% of salt, 5%-8% of green vegetables and 0.2%-0.5% of royal jelly, and the mass percentage of the components is 100%. At the same time, the present invention also discloses a preparation method of the artificial compound feed. The artificial compound feed for the tenebrio molitor is rich in various nutrient components, proteins, carbohydrates, etc. The salt and compound vitamin can maintain basic life activities of the tenebrio molitor, and the royal jelly is rich in nutrients and has very good functions of preventing bacteria and enhancing immunity. By using the feed to breed adult tenebrio molitor, egg laying amount of the adult tenebrio molitor is increased, hatching rate is increased and diseases are greatly reduced. By using the feed to breed tenebrio molitor larvae, individual weight of the larvae is increased significantly, mortality rate is decreased, pupation rate is increased, and diseases are greatly reduced. The preparation method is simple and easy for commercialization.

The invention relates to a feeding matrix suitable for 3-instar or older ghostmoth larvae and a cultivation method. The feeding matrix comprises vermiculite, river sand and coco coir, the raw materials are prepared into different proportions, so that the growth and development of the ghostmoth larvae in different ages can be met. According to the cultivation method disclosed by the invention, thefeeding matrix is utilized, and regular replacement and supplement of the feeding matrix is carried out until the ghostmoth larvae completely pupates. The method disclosed by the invention has the beneficial effects that the aged larvae can be subjected to pupation, eclosion and mating and oviposition through feeding, so that a stable ghostmoth source is provided for indoor feeding; the vitality of the bred ghostmoth larvae larvae is guaranteed.

The invention provides a tenebrio molitor breeding method. The method comprises the steps that firstly, an insect species of tenebrio molitor is selected; secondly, an appropriate breeding box is selected; thirdly, indoor tenebrio molitor larvae are bred; fourthly, amplification and propagation of tenebrio molitor are conducted. By means of the tenebrio molitor breeding method, a large batch of tenebrio molitor basically consistent in development can be established, and dry tenebrio molitor excrement is collected. The method overcomes the phenomena that in the artificial breeding process of tenebrio molitor, growth is slow, pupation fails, fecundity is low and stress resistance is poor, increases the daily gain and the pupation rate of tenebrio molitor, improves the quality of tenebrio molitor, can achieve vegetable straw harmless disposal, and improves economic benefits of breeding.

The invention discloses a method for artificially rearing athetis lepigone for test. According to the method for artificially rearing the athetis lepigone for test, artificially synthesized fodder is used for feeding the athetis lepigone, the method for artificially rearing the athetis lepigone for test is adopted in a matching mode at the same time, the survival rate of the athetis lepigone, the pupation rate of the athetis lepigone, the eclosion rate of the athetis lepigone and the egg laying amount of single female athetis lepigone are improved greatly, and a favorable condition is offered to the regular supply of the athetis lepigone for test. Meanwhile, the sensitivity of the athetis lepigone to a test is improved, and therefore the accuracy rate and the realness of a biotic experiment are improved. Moreover, according to the method for artificially rearing the athetis lepigone for test, due to the fact that the artificially synthesized fodder is adopted, the athetis lepigone is reared without the limitation of seasons, and rearing consistency is improved.

The invention relates to an automatic production line and an automatic production method for large-scale expanding propagation of noctuid larvae. The automatic production line for large-scale expanding propagation of the noctuid larvae comprises a conveying device, a feed filling device which is used for filling noctuid feed into feeding holes of noctuid feeding trays, a cooling device which is used for cooling the noctuid feeding trays filled with the noctuid feed to a preset temperature, a noctuid larva sub-packaging device which is used for uniformly mixing the noctuid larvae with a mediumto form a noctuid larva sub-packaging material and quantitatively putting the noctuid larva sub-packaging material into the feeding holes of the noctuid feeding trays, and a sealing device which is used for pasting sealing films with air holes on the noctuid feeding trays so as to seal the feeding holes, wherein the feed filling device, the cooling device, the noctuid larva sub-packaging device and the sealing device are sequentially distributed in the front-back direction of the automatic production line for large-scale expanding propagation of the noctuid larvae. According to the automatic production line for large-scale expanding propagation of the noctuid larvae, the automation degree of noctuid larva subpackaging is improved, and the subpackaging efficiency is improved.

The invention discloses a pupation method for black solider fly larvae. The method comprises the steps that black solider fly larva prepupae are flatly laid on sawdust with the mass water content being 30-60% and then placed at the environment under the temperature of 25-34 DEG C and relative humidity of 65-80% to be cultured, the black solider fly larva prepupae climb into sawdust to search for appropriate temperature, humidity and pressure environment for standing metamorphosis, and after 4-7 days of metamorphosis, the black solider fly larvae become pupas. Compared with black solider fly larva prepupae not treated in sawdust, the pupation time is greatly shortened, the pupation rate is improved, uniformity is high, and therefore production efficiency is greatly improved.

The invention provides a method for efficiently and intensively collecting natural enemy oligota sp. insect pupae. The collection method sequentially comprises the following steps that A1, an inversetruncated-cone-shaped container with two openings in the two ends and a cover is taken, the natural enemy oligota sp. insect larvae at the age of three days are placed on leaves with preys, and the leaves are put into the inverse truncated-cone-shaped container; A2, a culture dish is taken, and a gypsum block with small holes is placed in the culture dish; the inverse truncated-cone-shaped container is vertically placed in the culture dish, the gypsum block is inversely placed in the inverse truncated-cone-shaped container; then the top of the inverse truncated-cone-shaped container is coveredwith the container cover; A3, two days later, the natural enemy oligota sp. insect pupae are collected into the small holes of the gypsum block of the culture dish, and after the inverse truncated-cone-shaped container is removed, the gypsum block with the insect pupae inside is taken out. By means of the method, by utilizing two important habits that natural enemy oligota sp. insects fall downwards (to the ground) to pupate and find a hidden space to pupate, the insect pupae can be efficiently collected.

The invention discloses a method for breaking diapause of diapause larvae of monochamus alternatus. The method comprises the following steps: S01, preparing NaCL feed; and S02, under the conditions that the temperature ranges from 25 DEG C to 27 DEG C and the humidity ranges from 70% to 80%, placing the aged diapause larvae of the monochamus alternatus into an incubator, and feeding the diapause larvae of the monochamus alternatus with the NaCL feed. According to the scheme, a specific feed formula and a specific feeding environment are adopted, and a diapause breaking test of the diapause larvae of the monochamus alternatus shows that the pupation rate of the diapause larvae of the monochamus alternatus is greatly increased, so that the monochamus alternatus can better grow and breed in an artificial environment. The scheme is suitable for breaking diapause of the diapause larvae of the monochamus alternatus in winter, so that the monochamus alternatus larvae normally develop, the life history of the monochamus alternatus larvae is not limited by growth seasons, and the monochamus alternatus larvae can normally grow and develop in one year. A good foundation is laid for realizing subculture feeding of the monochamus alternatus and constructing an experimental population under an artificial condition.

The invention relates to an artificial feed for promoting growth of lepidoptera insects. The artificial feed comprises soybean meal, yeast powder, vitamin C, agar, linseed oil, 36 vol% acetic acid, aureomycin, sorbic acid, sodium diacetate and water. The artificial feed provided by the invention can promote the growth and development of target insect larvae, promote the pupation rate and eclosion rate of the larvae, and improve the egg laying rate of adults and the hatching rate of eggs. Meanwhile, the feed is not prone to mildewing and rotting in the later period, and the method is suitable for artificial mass feeding of indoor target insect larvae. The artificial feed disclosed by the invention is prepared by simple artificial stirring and uniform mixing, is rapid in forming, simple to operate, economical and environment-friendly, and has remarkable ecological, economic and social benefits.

The invention discloses an artificial feeding method for chlorophorus diadema motschulsky. The artificial feeding method includes (1) obtaining virginal adults and eggs; (2) managing hatching of the eggs indoors; (3) feeding artificially indoors; and the like. A test result shows that the artificial feeding method for the chlorophorus diadema motschulsky has the advantages that egg laying amount,pupation rate and eclosion rate are remarkably increased; developmental duration can be shortened by about four times; the weights and the lengths of larva and adults are increased, and survival rateis high; a large quantity of chlorophorus diadema motschulsky with uniform development and consistent physiology can be obtained continuously without being limited by seasons; and the like.

The invention belongs to the technical field of natural enemy insect breeding, and particularly relates to an indoor breeding method for Microplitis prodeniae Rao and Kurian. The method comprises the following steps: (1) sowing rice seeds and raising seedlings for later use; (2) inoculating larvae of spodoptera frugiperda or prodenia litura on the bred rice seedlings; (3) putting the rice seedlings together with larvae of spodoptera litura or prodenia litura into an insect breeding cage, and culturing indoors; (4) inoculating male and female Microplitis prodeniae Rao and Kurian to enable the Microplitis prodeniae Rao and Kurian to parasitize; (5) removing the Microplitis prodeniae Rao and Kurian; (6) feeding the parasitized larvae in the step (4) until the parasitic wasp mature larvae get out of the wall of the host body, and conducting spinning, cocooning and pupation; (7) picking out cocoons of the Microplitis prodeniae Rao and Kurian, putting the cocoons into a finger-shaped tube, and waiting for eclosion of the cocoons of the Microplitis prodeniae Rao and Kurian; and (8) repeating the steps (1)-(7) to carry out subculture breeding on the Microplitis prodeniae Rao and Kurian. According to the method, the indoor artificial propagation of the Microplitis prodeniae Rao and Kurian is realized.

The invention provides a method for breeding beet armyworm. Different beet armyworm unit area breeding amounts are set for different beet armyworm growing stages, and thus healthy and safe growth of beet armyworm is promoted. Feed is prepared from the main ingredients of corn flour, yeast powder, citric acid, sodium benzoate, saccharose, vitamin C, folium mori, radix angelica sinensis powder, semen cassiae powder and water. The two traditional Chinese medicine materials of radix angelica sinensis powder and semen cassiae powder are added on the basis of traditional feed for beet armyworm and can improve the immunity of beet armyworm in combination with folium mori, and thus the effect of increasing the larva survival rate, pupation rate and eclosion rate of beet armyworm is achieved; detection shows that the propagation efficiency of beet armyworm bred with the feed can be improved by 10% or above.