45results about How to "Increase pupation rate" patented technology

The invention relates to an artificial feeding method of tested insect, especially relates to an artificial feeding method of tested chive maggot, wherein the Chinese chive feedstuff is replaced by the artificial synthetic feedstuff, thus the survival rate, pupation rate, emergence rate, single-female fecundity are greatly increased compared with the prior tested chive maggot breeding method, therefore the good condition is provided for regular supply of tested chive maggot.

The invention provides a semi-artificial feed for Spodoptera frugiperda and a preparation method of the semi-artificial feed and a method for feeding the Spodoptera frugiperda. The semi-artificial feed comprises soybean meal (7.00%-9.00%), yeast meal (3.00%-4.50%), corn meal (7.50%-9.50%), corn oil (0.20%-0.30%), ascorbic acid (0.20%-0.30%), sorbic acid (0.10%-0.20%), methyl parahydroxybenzoate (0.10%-0.20%), cholesterol (0.060%-0.065%), agar (1.00%-1.50%), and water (75.00%-80.00%), base on weight percentage. The feed provided by the invention has low cost, wide source of feed raw materials,and simple and convenient preparation, is suitable for growth, development and reproduction of the Spodoptera frugiperda, can be used for group feeding, and thus can realize long-term successive large-scale feeding of the Spodoptera frugiperda (large-scale feeding with successive generations). Moreover, the preparation method of the semi-artificial feed for Spodoptera frugiperda is provided.

The invention discloses a barley pest breeding method. The method comprises the following steps: preparation of feed, preparation of a breeding box, preparation of an incubator, feed casting, egg collection and hatching as well as feeding management. When barley pests are bred with the method, mutual interference and killing under the condition of social feeding can be reduced effectively; through strict control of feed and breeding density, the percentage of pupation of the barley pests is increased while the nutrients required by growth of the barley pests is guaranteed, the economic benefit is increased, and the excellent barley pest breeding method has the advantages of low labor intensity, high percentage of pupation, low cost and the like.

The invention discloses artificial synthesized feed for proxenuslepigone moschler and a preparation method thereof. The feed adopts wheat bran and mushroom powder as main components and comprises whole meal flour, peptone, and other auxiliary materials. The proxenuslepigone moschler which is fed by the feed in a certain ratio has significantly improved survival rate, pupation rate, eclosion rate and single-female egg laying amount. The feed cannot be influenced by seasons and is free from pesticide or other residual components. In addition, the feed is prepared by a microwave oven with simple operation, the preparation amount can be controlled and the feed can be prepared when needed, thereby improving the feed utilization rate and solving a problem that too much feed is prepared for one time and needs to be stored in a refrigerator.

The invention relates to a large-scale artificial feeding method for tested insects, in particular to a large-scale artificial feeding method for tested bradysia odoriphaga. Conventional leeks, green Chinese onions and artificial feeds are replaced with peanuts, and agar moisture preservation and manual water addition moisture preservation methods are replaced with fine sand. Compared with the conventional feeding method for tested bradysia odoriphaga, the method has the advantages that equivalent survival rate, pupation rate, eclosion rate and egg number per female are achieved, the cost is reduced, manual operation steps are reduced, and favorable conditions are created for the normal supply of the tested bradysia odoriphaga.

The invention discloses an artificial feed for diamondback moths, and the feed consists of corn germ powder, new cabbage leaf powder, casein, spiral seaweed powder, cholesterol, fresh lemon juice, choline chloride, glucosinolate, and thyme essential oil. The artificial feed can improve the immunity, disease resistance and adaptability of young and adult diamondback moths, thereby improving the percentage of pupation, eclosion rate and egg laying amount, shortening the larva period, prolonging the service life of adults, and achieving the large-scale, standardization and high-efficiency indoor cultivation of diamondback moths.

The invention discloses a method for reproducing stegobium paniceum (L.) by utilizing semi-artificial feed, belongs to an insect reproduction method, and aims to provide a stegobium paniceum (L.) reproduction method which is uniform in insect growth and development and good in physiological status consistency. The method comprises the steps of egg collection, egg hatching and larvae rearing. Specifically, the method comprises the following steps: putting adults into a culture dish loaded with feed, sealing an opening of the culture dish with a preservative film with vent holes, and then putting the culture dish into a constant-temperature environment; collecting eggs, putting the eggs between two layers of preservative films, and then hatching the eggs in the constant-temperature environment; and after the eggs are hatched into larvae, putting the larvae into a new culture dish loaded with feed, forming a plurality of small holes in the surface of the feed by pricking, sealing an opening of the culture dish with a preservative film with vent holes, and then performing culture in the constant-temperature environment until the larvae grow into adults. The method is simple to operate; the egg hatching rate, the survival rate, the pupation rate and the like are all remarkably increased; and feed raw materials are easily available. The method is the stegobium paniceum (L.) reproduction method.

The present invention discloses an artificial compound feed for tenebrio molitor. The feed is characterized by comprising the following components in mass percentages: 70%-74% of wheat bran, 6%-8% of corn flour, 2%-4% of soybean powder, 4%-6% of milk, 2%-6% of white sugar, 0.3%-0.7% of compound vitamin, 2.2%-2.5% of salt, 5%-8% of green vegetables and 0.2%-0.5% of royal jelly, and the mass percentage of the components is 100%. At the same time, the present invention also discloses a preparation method of the artificial compound feed. The artificial compound feed for the tenebrio molitor is rich in various nutrient components, proteins, carbohydrates, etc. The salt and compound vitamin can maintain basic life activities of the tenebrio molitor, and the royal jelly is rich in nutrients and has very good functions of preventing bacteria and enhancing immunity. By using the feed to breed adult tenebrio molitor, egg laying amount of the adult tenebrio molitor is increased, hatching rate is increased and diseases are greatly reduced. By using the feed to breed tenebrio molitor larvae, individual weight of the larvae is increased significantly, mortality rate is decreased, pupation rate is increased, and diseases are greatly reduced. The preparation method is simple and easy for commercialization.

The invention relates to a feeding matrix suitable for 3-instar or older ghostmoth larvae and a cultivation method. The feeding matrix comprises vermiculite, river sand and coco coir, the raw materials are prepared into different proportions, so that the growth and development of the ghostmoth larvae in different ages can be met. According to the cultivation method disclosed by the invention, thefeeding matrix is utilized, and regular replacement and supplement of the feeding matrix is carried out until the ghostmoth larvae completely pupates. The method disclosed by the invention has the beneficial effects that the aged larvae can be subjected to pupation, eclosion and mating and oviposition through feeding, so that a stable ghostmoth source is provided for indoor feeding; the vitality of the bred ghostmoth larvae larvae is guaranteed.

The invention discloses a method for artificially rearing athetis lepigone for test. According to the method for artificially rearing the athetis lepigone for test, artificially synthesized fodder is used for feeding the athetis lepigone, the method for artificially rearing the athetis lepigone for test is adopted in a matching mode at the same time, the survival rate of the athetis lepigone, the pupation rate of the athetis lepigone, the eclosion rate of the athetis lepigone and the egg laying amount of single female athetis lepigone are improved greatly, and a favorable condition is offered to the regular supply of the athetis lepigone for test. Meanwhile, the sensitivity of the athetis lepigone to a test is improved, and therefore the accuracy rate and the realness of a biotic experiment are improved. Moreover, according to the method for artificially rearing the athetis lepigone for test, due to the fact that the artificially synthesized fodder is adopted, the athetis lepigone is reared without the limitation of seasons, and rearing consistency is improved.

The invention discloses a method for improving the insect resistance capability of hybrid poplar, which comprises the following steps: obtaining SEQ ID No.1 through RT-PCR with the mRNA of the leaves of poplar as a template and SEQ ID No.2 and No.3 as primers and connecting pMD18-T, leading the obtained recombinant plasmid into E.coli strain DH5alpha, screening positive clones, extracting plasmid and carrying out double enzyme digestion, connecting the SEQ ID No.1 and pBI121 subjected to double enzyme digestion, transforming E.coli strain DH5alpha, screening bacterial colony containing resistance plasmid, extracting plasmid for PCR verification and double enzyme digestion verification, choosing the correct recon, naming the correct recon as pBI121-POP3 and transferring the correct recon into agrobacterium tumefaciens for colony PCR identification; transforming the hybrid poplar by the obtained strain to obtain transgenic seedlings. The method provided by the invention can improve the insect resistance capability of the hybrid poplar.

The invention relates to an automatic production line and an automatic production method for large-scale expanding propagation of noctuid larvae. The automatic production line for large-scale expanding propagation of the noctuid larvae comprises a conveying device, a feed filling device which is used for filling noctuid feed into feeding holes of noctuid feeding trays, a cooling device which is used for cooling the noctuid feeding trays filled with the noctuid feed to a preset temperature, a noctuid larva sub-packaging device which is used for uniformly mixing the noctuid larvae with a mediumto form a noctuid larva sub-packaging material and quantitatively putting the noctuid larva sub-packaging material into the feeding holes of the noctuid feeding trays, and a sealing device which is used for pasting sealing films with air holes on the noctuid feeding trays so as to seal the feeding holes, wherein the feed filling device, the cooling device, the noctuid larva sub-packaging device and the sealing device are sequentially distributed in the front-back direction of the automatic production line for large-scale expanding propagation of the noctuid larvae. According to the automatic production line for large-scale expanding propagation of the noctuid larvae, the automation degree of noctuid larva subpackaging is improved, and the subpackaging efficiency is improved.

The invention provides a method for efficiently and intensively collecting natural enemy oligota sp. insect pupae. The collection method sequentially comprises the following steps that A1, an inversetruncated-cone-shaped container with two openings in the two ends and a cover is taken, the natural enemy oligota sp. insect larvae at the age of three days are placed on leaves with preys, and the leaves are put into the inverse truncated-cone-shaped container; A2, a culture dish is taken, and a gypsum block with small holes is placed in the culture dish; the inverse truncated-cone-shaped container is vertically placed in the culture dish, the gypsum block is inversely placed in the inverse truncated-cone-shaped container; then the top of the inverse truncated-cone-shaped container is coveredwith the container cover; A3, two days later, the natural enemy oligota sp. insect pupae are collected into the small holes of the gypsum block of the culture dish, and after the inverse truncated-cone-shaped container is removed, the gypsum block with the insect pupae inside is taken out. By means of the method, by utilizing two important habits that natural enemy oligota sp. insects fall downwards (to the ground) to pupate and find a hidden space to pupate, the insect pupae can be efficiently collected.

The invention relates to an artificial feed for promoting growth of lepidoptera insects. The artificial feed comprises soybean meal, yeast powder, vitamin C, agar, linseed oil, 36 vol% acetic acid, aureomycin, sorbic acid, sodium diacetate and water. The artificial feed provided by the invention can promote the growth and development of target insect larvae, promote the pupation rate and eclosion rate of the larvae, and improve the egg laying rate of adults and the hatching rate of eggs. Meanwhile, the feed is not prone to mildewing and rotting in the later period, and the method is suitable for artificial mass feeding of indoor target insect larvae. The artificial feed disclosed by the invention is prepared by simple artificial stirring and uniform mixing, is rapid in forming, simple to operate, economical and environment-friendly, and has remarkable ecological, economic and social benefits.

The invention provides a method for breeding beet armyworm. Different beet armyworm unit area breeding amounts are set for different beet armyworm growing stages, and thus healthy and safe growth of beet armyworm is promoted. Feed is prepared from the main ingredients of corn flour, yeast powder, citric acid, sodium benzoate, saccharose, vitamin C, folium mori, radix angelica sinensis powder, semen cassiae powder and water. The two traditional Chinese medicine materials of radix angelica sinensis powder and semen cassiae powder are added on the basis of traditional feed for beet armyworm and can improve the immunity of beet armyworm in combination with folium mori, and thus the effect of increasing the larva survival rate, pupation rate and eclosion rate of beet armyworm is achieved; detection shows that the propagation efficiency of beet armyworm bred with the feed can be improved by 10% or above.