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Method for breaking diapause of diapause larvae of monochamus alternatus

A technology for diapause and diapause of A. pineapple, applied in food processing, animal husbandry, additional food elements, etc., can solve problems such as diapause of larvae, achieve the effects of good growth and reproduction, and improve the rate of pupation

Active Publication Date: 2021-07-23
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problem that Monochamus alternatus larvae resist unfavorable factors in the diapause state in winter, the purpose of the present invention is to provide a method for breaking diapause of Monochamus alternatus larvae, through a special feeding formula to achieve breaking diapause The effect of avoiding the existing monochamus monochamus larvae in the normal feed feeding situation of a large number of diapause of larvae defects

Method used

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  • Method for breaking diapause of diapause larvae of monochamus alternatus
  • Method for breaking diapause of diapause larvae of monochamus alternatus
  • Method for breaking diapause of diapause larvae of monochamus alternatus

Examples

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Effect test

Embodiment 1

[0032] This embodiment provides an artificial feed for breaking diapause of Monochamus alternatus larvae, comprising the following components: 300ml sterile water, 30g agar, 40g sucrose, 60g wheat bran, 4g sodium benzoate, 2g sorbic acid, 10g shrimp Powder, 25g dry yeast, 150g sawdust.

[0033] Based on the above-mentioned feed ingredients, the present embodiment provides a method for preparing the above-mentioned feed for breaking diapause of Monochamus alternatus larvae, comprising the following steps:

[0034] (1) 300mL sterile water for standby;

[0035] (2) Pulverize the pine wood material and the phloem respectively, and measure the required raw materials of each component for subsequent use;

[0036] (3) Pour 300ml of 50mmol / L NaCl solution into the pot, add 30g of agar and stir until it melts;

[0037] (4) Add 40g of sucrose, 60g of wheat bran, 4g of sodium benzoate, 2g of sorbic acid, 10g of shrimp powder, 25g of dry yeast, and 150g of sawdust and stir until it beco...

Embodiment 2

[0040] The present embodiment provides a kind of artificial feed for breaking diapause of Monochamus alternatus larvae, comprising the following components: 300ml of 10mmol / L NaCl solution, 30g agar, 40g sucrose, 60g wheat bran, 4g sodium benzoate, 2g sorbic acid, 10g shrimp powder, 25g dry yeast, 150g sawdust.

[0041] Based on the above-mentioned feed formula, the present embodiment provides the preparation method of corresponding artificial feed, which comprises the following steps:

[0042](1) Prepare 10mmol / L NaCl solution with NaCl medicine and sterile water, and take 300mL for later use;

[0043] (2) Pulverize the pine wood material and the phloem respectively, and measure the required raw materials of each component for subsequent use;

[0044] (3) Pour 300ml of 10mmol / L NaCl solution into the pot, add 30g of agar and stir until it melts;

[0045] (4) Add 40g of sucrose, 60g of wheat bran, 4g of sodium benzoate, 2g of sorbic acid, 10g of shrimp powder, 25g of dry yea...

Embodiment 3

[0048] A kind of artificial feed for breaking diapause of Monochamus alternatus larvae in this embodiment includes the following components: 50mmol / L NaCl solution of 300ml, 30g agar, 40g sucrose, 60g wheat bran, 4g sodium benzoate, 2g sorbic acid, 10g Shrimp powder, 25g dry yeast, 150g sawdust.

[0049] Based on the above-mentioned feed formula, the present embodiment provides the preparation method of corresponding artificial feed, which comprises the following steps:

[0050] (1) Prepare 50mmol / L NaCl solution with NaCl medicine and sterile water, and take 300mL for later use;

[0051] (2) Pulverize the pine wood material and the phloem respectively, and measure the required raw materials of each component for subsequent use;

[0052] (3) Pour 300ml of 50mmol / L NaCl solution into the pot, add 30g of agar and stir until it melts;

[0053] (4) Add 40g of sucrose, 60g of wheat bran, 4g of sodium benzoate, 2g of sorbic acid, 10g of shrimp powder, 25g of dry yeast, and 150g of...

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Abstract

The invention discloses a method for breaking diapause of diapause larvae of monochamus alternatus. The method comprises the following steps: S01, preparing NaCL feed; and S02, under the conditions that the temperature ranges from 25 DEG C to 27 DEG C and the humidity ranges from 70% to 80%, placing the aged diapause larvae of the monochamus alternatus into an incubator, and feeding the diapause larvae of the monochamus alternatus with the NaCL feed. According to the scheme, a specific feed formula and a specific feeding environment are adopted, and a diapause breaking test of the diapause larvae of the monochamus alternatus shows that the pupation rate of the diapause larvae of the monochamus alternatus is greatly increased, so that the monochamus alternatus can better grow and breed in an artificial environment. The scheme is suitable for breaking diapause of the diapause larvae of the monochamus alternatus in winter, so that the monochamus alternatus larvae normally develop, the life history of the monochamus alternatus larvae is not limited by growth seasons, and the monochamus alternatus larvae can normally grow and develop in one year. A good foundation is laid for realizing subculture feeding of the monochamus alternatus and constructing an experimental population under an artificial condition.

Description

technical field [0001] The invention relates to the technical field of artificial breeding of Monochamus alternatus, in particular to a method for breaking diapause of Monochamus alternatus larvae. Background technique [0002] Monochamus alternatus, also known as Monochamus alternatus, belongs to the genus Mochamonis genus of Coleoptera Cyperopteridae, and is distributed in Fujian, Liaoning, Hebei, Jiangxi, Anhui, Henan, Shaanxi, Shandong, Hunan, and Hubei in China. , Jiangsu, Zhejiang, Guangdong, Guangxi, Sichuan, Yunnan, Guizhou, Tibet, Taiwan, Hong Kong and other places, and abroad are distributed in Japan, North Korea, Laos, Vietnam and other countries. Monochamus alternatus is a major stem-boring pest. The 1-2 instar larvae of Monochamus alternata feed on the phloem under the epidermis of pine trees, destroying the nutrient and water transportation channels of pine trees, causing tree vigor to weaken and growth to be hindered;3 The post-age larvae drill into the boreh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A23K50/90A23K20/22A23K20/163A23K10/37A23K20/111A23K20/105A23K10/22A23K10/18A23K10/30
CPCA01K67/033A23K50/90A23K20/22A23K20/163A23K10/37A23K20/111A23K20/105A23K10/22A23K10/18A23K10/30Y02P60/87
Inventor 胡霞杨梅娇李国强初旭马秋雨张飞萍吴松青王荣
Owner FUJIAN AGRI & FORESTRY UNIV
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