Method for artificially feeding drosophila suzukii matsumura

Abstract

The invention discloses a method for artificially feeding drosophila suzukii matsumura, wherein adults which are feathered in the same day are selected to put into a self-made adult feeding cage, 5.5g artificial feed is stored in a culture dish A, then the culture dish A is arranged in a culture dish B which is paved with a sterilization filter paper, sterile water is injected on the sterilization filter paper which is arranged in the culture dish B, the culture dish B which is matched with the culture dish A is put into the self-made adult feeding cage, and is put into a phytotron to observe and count number of egg grains, the culture dish A is put into a glass beaker, and is sealed through a Para film and then is put into the phytotron to feed, the growth, the development and the feeding behavior of feed insects are observed in a stereomicroscope, are observed until larva are aged in fixed time every day, the adults which are feathered in the same day are conjugated according to the female-male ratio of 1:1, and the conjugated adults are respectively put into the adult feeding cage to feed. The method for artificially feeding the drosophila suzukii matsumura is simple, convenient in operation and easy to feed targeted insects.

Description

technical field [0001] The invention belongs to the technical field of insect feeding, and relates to an artificial feeding method of Drosophila suzuki. Background technique [0002] Suzuki's fruit fly (Drosophilasuzukii Matsumura), also known as cherry fruit fly, spotted winged fruit fly, belongs to Diptera (Diptera) Ring suborder (Cylorrhapha) Drosophilidae (Drosophilidae) Drosophila (Drosophila) fruit fruit fly subgenus (Subgenus Sophophora). [0003] Drosophila suzuki is a complete metamorphosis insect, including four stages of egg, larva, pupa and adult. Due to the small size of the worm and its relatively hidden place of life, it is very difficult to be found in the early and early stages of the worm's damage. Adults are small in size (about 2-3 mm in length) and soft in body. Adults tend to choose the hidden gaps and wounds of fruits for feeding and laying eggs, or female adults choose ripe fruits to lay eggs directly under the skin; newly hatched larvae directly la...

AI Technical Summary

Problems solved by technology

[0009] 1. Due to regional differences, there are differences in the varieties of materials (apples and bananas, etc.) used in experiments, resulting in poor breeding effects

[0010] 2. There may also be some differences in the feed production procedures, which also lead to unsatisfactory feeding effects (the publicized materials do not have feed production procedures, and there is no moisture content parameter added to the feed)

[0011] 3. Due to differences in regions and test time, the inventor of this paper has gone through many tests in the laboratory, and the results show that the method is not ideal for moisturizing the feed surface, resulting in the feed surface being easy to dry and lose water, which is not conducive to the feeding of fruit flies and spawn

In addition, agar is added to the feed, and the formed feed is in the form of a solid block. The surface of the feed is easy to air dry and affects the feeding and oviposition of fruit flies. The effect is not ideal

[0012] 4. Due to the particularity of the egg-laying habits of Drosophila Suzuki (eggs laid under the fruit skin, eggs laid in fruit gaps, and fruit wounds), the solid feed has a certain hardness, and the test shows that its eggs are mostly produced in the feed surface, which is not conducive to the hatching of eggs

The solid agar feed restricts the moth and activity of the larvae, and is not conducive to observing the biological characteristics of the larvae at any time

If there is a need for observation, the solid feed must be cut open, which not only destroys the living environment of the insect, but also easily damages the target insect body, affecting the growth and development of the insect, thereby affecting the test effect

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