Preparation method of corn protein active peptide carried calcium ion nano chelate

Abstract

The invention solves the problems of poor solubility, low absorption and utilization rate, low product functionality, high preparation cost and the like of chelated calcium. The preparation method of the corn protein active peptide carried calcium ion nano chelate comprises the following steps: 1, carrying out enzyme hydrolysis on zein to obtain corn protein active peptide; 2, separating and screening corn active peptide with specific molecular weight and good chelating effect with calcium ions; and 3, preparing the peptide calcium nano chelate by taking the corn peptide as a carrier. The invention has the advantages that: 1, the nutritional value and bioavailability of calcium element are obviously improved; 2, the particle size is small, the solubility is high, and the stability is good; and 3, the safety is high, and the application prospect is wide. The preparation method of the corn protein active peptide carried calcium ion nano chelate is high in calcium ion chelating efficiency, good in controlled release property and strong in nutrition functionality.

Description

Technical field:

[0001] The invention relates to a preparation method of a zein active peptide-carrying calcium ion nano-chelate. Background technique:

[0002] As an essential nutrient, calcium plays an extremely important role in the human body. Calcium directly participates in the regulation of many physiological activities such as the human respiratory system, endocrine system, nervous system, and immune system. When the human body’s calcium intake is insufficient or the bioavailability is low, the body will cause calcium deficiency, resulting in rickets, bone Occurrence of diseases such as looseness. The human body can only maintain the calcium balance in the body through diet. The calcium content required by normal adults is 800-1000 mg per day. According to the latest monitoring data on the nutrition and health status of Chinese residents, the per capita daily calcium intake of urban and rural residents in my country is 366.1mg, which is less than 50% of the recomme...

Examples

specific Embodiment approach 1

[0025] Specific embodiment one: a kind of preparation method of zein active peptide carrying calcium ion nano-chelate is realized through the following steps:

[0026] 1, a kind of preparation method of zein active peptide carrying calcium ion nano-chelate, it is characterized in that: the method comprises the following steps:

[0027] (1) Enzymatic hydrolysis preparation of zein peptide

[0028] Disperse the corn gluten powder into 10-20 times the volume of deionized water, adjust the pH to 3.0, add protease with 0.5%-3.0% protein powder mass, and enzymatically hydrolyze at 40-60°C for 1-6 hours. After the enzyme reaction, cook at 95°C for 5 minutes to inactivate the enzyme. After centrifugation, the supernatant was taken to obtain the enzymatic hydrolyzate of zein peptide.

[0029] (2) Separation and screening of corn active peptides

[0030] Centrifuge the above zein peptide enzymatic hydrolysis solution at 4000r / min for 20min, then use ultrafiltration membranes with dif...

specific Embodiment approach 2

[0033]Specific embodiment two: The difference between this embodiment and specific embodiment one is that step (1) disperses zein powder with a purity greater than 95% into deionized water of 10 to 20 times the volume, and adds 1.0% protein powder mass ~2.0% pepsin, hydrolyze at 50~55℃ for 3~4 hours. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0034] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step (1), ultrafiltration membranes with molecular weight cut-offs of 8000Da and 1000Da are respectively used to obtain corn peptide solutions with molecular weights of 1000-8000Da. Other steps are the same as in the first embodiment.

Technical field:

[0001] The invention relates to a preparation method of a zein active peptide-carrying calcium ion nano-chelate. Background technique:

[0002] As an essential nutrient, calcium plays an extremely important role in the human body. Calcium directly participates in the regulation of many physiological activities such as the human respiratory system, endocrine system, nervous system, and immune system. When the human body’s calcium intake is insufficient or the bioavailability is low, the body will cause calcium deficiency, resulting in rickets, bone Occurrence of diseases such as looseness. The human body can only maintain the calcium balance in the body through diet. The calcium content required by normal adults is 800-1000 mg per day. According to the latest monitoring data on the nutrition and health status of Chinese residents, the per capita daily calcium intake of urban and rural residents in my country is 366.1mg, which is less than 50% of the recomme...

Examples

specific Embodiment approach 1

[0025] Specific embodiment one: a kind of preparation method of zein active peptide carrying calcium ion nano-chelate is realized through the following steps:

[0026] 1, a kind of preparation method of zein active peptide carrying calcium ion nano-chelate, it is characterized in that: the method comprises the following steps:

[0027] (1) Enzymatic hydrolysis preparation of zein peptide

[0028] Disperse the corn gluten powder into 10-20 times the volume of deionized water, adjust the pH to 3.0, add protease with 0.5%-3.0% protein powder mass, and enzymatically hydrolyze at 40-60°C for 1-6 hours. After the enzyme reaction, cook at 95°C for 5 minutes to inactivate the enzyme. After centrifugation, the supernatant was taken to obtain the enzymatic hydrolyzate of zein peptide.

[0029] (2) Separation and screening of corn active peptides

[0030] Centrifuge the above zein peptide enzymatic hydrolysis solution at 4000r / min for 20min, then use ultrafiltration membranes with dif...

specific Embodiment approach 2

[0033]Specific embodiment two: The difference between this embodiment and specific embodiment one is that step (1) disperses zein powder with a purity greater than 95% into deionized water of 10 to 20 times the volume, and adds 1.0% protein powder mass ~2.0% pepsin, hydrolyze at 50~55℃ for 3~4 hours. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0034] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step (1), ultrafiltration membranes with molecular weight cut-offs of 8000Da and 1000Da are respectively used to obtain corn peptide solutions with molecular weights of 1000-8000Da. Other steps are the same as in the first embodiment.

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