A simple automatic dyeing machine

Abstract

The present invention discloses a simple automatic dyeing machine, comprising a supporting base plate, a lateral moving mechanism, a flipping mechanism, a glass slide holder, a liquid storage assembly, a waste liquid tank and a valve control pipeline, wherein the fixed part of the lateral moving mechanism is fixed on the supporting base plate, the fixed part of the flipping mechanism is fixed on the moving part of the lateral moving mechanism, the glass slide holder is fixed on the flipping part of the flipping mechanism, the waste liquid tank is placed on the supporting base plate, the glass slide holder is located directly above the waste liquid tank, the liquid storage assembly comprises a liquid storage tank and a liquid storage support frame, the liquid storage tank is installed directly above the supporting base plate through the liquid storage support frame, the liquid storage tank is divided into a first dyeing reagent storage unit, a second dyeing reagent storage unit ... an Nth dyeing reagent storage unit and a cleaning agent storage unit, and the valve control assembly comprises a first liquid supply assembly ... an Nth liquid supply assembly and an N+1th liquid supply assembly. The dyeing machine has a simple structure, a reasonable design, a compact structure, a relatively small volume, a relatively low price, and is suitable for large-scale promotion and application.

Description

Technical Field

[0001] The invention relates to the technical field of cell or pathological section pre-processing, and in particular to a simple automatic staining machine. Background Art

[0002] Traditional cell or pathology section pre-processing involves sample staining operations, which are generally performed by technicians with clinical experience, who manually process cell pathology samples with different reagents in accordance with the preparation and staining operation requirements to complete the sample staining process.

[0003] The automatic dyeing machines developed and designed based on the above process are mainly for large medical institutions. Therefore, this kind of dyeing machine is large in size, high in cost, and often sold at a high price. Equipment cost and volume factors are not conducive to the promotion of such large automatic dyeing devices, while clinical departments or primary medical institutions hope to have automatic dyeing devices that are small in size, low in price, and not inferior to traditional dyeing machines. Summary of the invention

[0004] Based on the above-mentioned prior art, the present invention provides a simple automatic dyeing machine, which has a simple structure, reasonable design, compact structure, relatively small size, relatively low price, and is suitable for large-scale promotion and application.

[0005] The technical solution adopted to achieve the above-mentioned purpose of the present invention is:

[0006] A simple automatic staining machine comprises a supporting bottom plate, a lateral moving mechanism, a flipping mechanism, a glass slide holder, a liquid storage component, a waste liquid tank and a valve-controlled pipeline, wherein a fixed part of the lateral moving mechanism is fixed to the supporting bottom plate, a fixed part of the flipping mechanism is fixed to a moving part of the lateral moving mechanism, the glass slide holder is fixed to a flipping part of the flipping mechanism, the glass slide holder can reciprocate in a horizontal direction and can flip, the waste liquid tank has an opening at the top, the waste liquid tank is arranged on the supporting bottom plate, the glass slide holder is always located directly above the waste liquid tank, the liquid storage component comprises a liquid storage tank and a liquid storage support frame, the liquid storage support frame is fixed to the supporting bottom plate, the liquid storage tank is fixed to the liquid storage support frame, and the liquid storage tank is divided into a first staining reagent storage unit, a second staining reagent storage unit ... an Nth staining reagent storage unit and a cleaning reagent storage unit;

[0007] The valve control assembly includes a first liquid supply assembly...an Nth liquid supply assembly and an N+1th liquid supply assembly, the first liquid supply assembly includes a first liquid supply pipeline and a first electromagnetic valve group, the first electromagnetic valve group includes a plurality of first electromagnetic valves distributed along a straight line perpendicular to the moving direction of the glass slide holder, the first liquid supply pipeline is connected to the liquid outlet of the first staining reagent unit, the first liquid supply pipeline is respectively connected to the plurality of first electromagnetic valves...the Nth electromagnetic valve group includes a plurality of Nth electromagnetic valves distributed along a straight line perpendicular to the moving direction of the glass slide holder, the Nth liquid supply pipeline is connected to the Nth staining reagent unit. The liquid outlet is connected, the Nth liquid supply pipeline is respectively connected to a plurality of Nth solenoid valves, the N+1th solenoid valve group includes a plurality of N+1th solenoid valves distributed along a straight line perpendicular to the moving direction of the glass slide holder, the N+1th liquid supply pipeline is connected to the liquid outlet of the cleaning reagent storage unit, and the N+1th liquid supply pipeline is respectively connected to a plurality of N+1th solenoid valves; the first solenoid valve group...the Nth solenoid valve group and the N+1th solenoid valve group are distributed along a straight line parallel to the moving direction of the glass slide holder, and the first solenoid valve...the Nth solenoid valve and the N+1th solenoid valve are distributed in a matrix;

[0008] The glass slide holder is located below the first solenoid valve group, the Nth solenoid valve group and the N+1th solenoid valve group. The glass slide holder is provided with multiple glass slide positions for mounting glass slides. The number of glass slide positions, the first solenoid valve...the Nth solenoid valve and the N+1th solenoid valve is equal. The straight lines where the moving trajectories of the multiple glass slide positions are located are respectively located directly below the straight lines where the rows or columns of the matrix are located.

[0009] The lateral moving mechanism is a lateral screw slide, which comprises a base, a slide and a screw. The base is fixed on the supporting bottom plate, and the turning mechanism is installed on the slide.

[0010] The lateral movement mechanism also includes a limit block, a first position sensor for sensing that the slide returns to an initial position, and a second position sensor for sensing that the slide reaches an end position. The first position sensor and the second position sensor are respectively installed on both sides of the base, and the limit block is installed on the slide. The limit block can trigger the first position sensor and the second position sensor respectively.

[0011] The flip mechanism comprises a flip motor and a flip assembly. The flip motor is fixed on the slide, the flip transmission assembly is connected to the output end of the flip motor, and the glass slide holder is installed on the flip assembly.

[0012] The flipping mechanism also includes a motor mounting plate, a connecting support plate, a reducer, a first bearing seat, a second bearing seat, a guide rail and a slider. The connecting support plate is horizontally fixed to the slide, the motor mounting plate is fixed to the connecting support plate, the motor mounting plate is perpendicular to the connecting support plate, the flipping motor and the reducer are both fixedly mounted on the outer side of the motor mounting plate, the output end of the flipping motor is connected to the input end of the reducer, the guide rail is mounted on the supporting bottom plate, the guide rail is parallel to the screw, and the guide rail and the screw are respectively located on both sides of the waste liquid tank, the slider is mounted on the guide rail, the first bearing seat is fixed to the connecting support plate, the second bearing is fixed to the slider, the flipping assembly includes a first flipping axis, a flipping support plate and a second flipping axis, the first flipping axis passes through the first bearing seat and the motor mounting plate, the first flipping axis is rotatably connected to the motor mounting plate, one end of the first flipping axis is connected to the output end of the reducer, the second flipping axis passes through the second bearing seat, the flipping support plate is located between the first bearing seat and the second bearing seat, and the flipping support plate is respectively connected to the other end of the first flipping axis and one end of the second flipping axis.

[0013] The glass slide holder comprises a bracket and a film making chamber, the bracket comprises a bracket, a spring clip and a support rod, the bracket is square-shaped, there are multiple support rods, the multiple support rods are evenly distributed between the bracket and the flip support plate, the lower end of each support rod is fixed to the flip support plate, the upper end of each support rod is fixed to the bottom of the bracket, the side walls and the top of the bracket in a length direction are open, a plurality of evenly distributed partitions are arranged in the bracket along its length direction, the bracket is divided into a plurality of glass slide placement grooves by the partitions, the openings on the side walls in the length direction of the bracket are divided into a plurality of glass slide placement grooves by the partitions, a pressure strip is arranged on the top opening of each glass slide placement groove, the number of the spring clips is equal to the number of the glass slide placement grooves, and each spring clip cooperates with the glass slide placement entrance of the corresponding glass slide placement groove.

[0014] The spring clamp comprises a clamping adjustment component and a baffle that can automatically reset. The clamping adjustment component comprises two fixing ears, a torsion spring and a clamping adjustment shaft. The two fixing ears are respectively fixed on the bottom of the bracket groove, and the two fixing ears are respectively located outside the bracket. The two ends of the clamping adjustment shaft are respectively rotatably connected with the two fixing ears. The torsion spring is sleeved on the middle part of the clamping adjustment shaft. One end of the torsion spring is fixedly connected with the clamping adjustment shaft. The baffle is L-shaped. One end of the baffle is respectively sleeved on both sides of the clamping adjustment shaft. The other end of the torsion spring is respectively sleeved with the baffle on the clamping The ends of the adjusting shaft are connected, and each clamping and adjusting assembly cooperates with the corresponding glass slide placement groove, and each clamping and adjusting assembly is fixed on the bottom of the corresponding glass slide placement groove. There are multiple film making chambers, and the number of film making chambers is equal to the number of glass slide placement grooves. When each film making chamber is placed on the corresponding glass slide placement groove, the corresponding torsion spring is in a natural state, a straight part of the corresponding baffle is close to the bottom of the corresponding glass slide placement groove, and the other straight part clamps the corresponding film making chamber, and each glass slide placement groove and the corresponding film making chamber constitute a glass slide position.

[0015] There are two slide placement slots, and the liquid storage box is square. The liquid storage box is divided into a cleaning reagent chamber, two first staining reagent chambers, two second staining reagent chambers, two third staining reagent chambers, two fourth staining reagent chambers, two fifth staining reagent chambers and a sixth staining reagent chamber. The cleaning reagent chamber, the first staining reagent chamber, the second staining reagent chamber, the third staining reagent chamber, the fourth staining reagent chamber, the fifth staining reagent chamber and the sixth staining reagent chamber are all square. The symmetry plane of the cleaning reagent chamber coincides with the symmetry plane of the liquid storage box. The two first staining reagent chambers are symmetrically distributed on both sides of the length direction of the cleaning reagent chamber. The two second staining reagent chambers are symmetrically distributed on both sides of the length direction of the cleaning reagent chamber. The two third staining reagent chambers are symmetrically distributed on both sides of the length direction of the cleaning reagent chamber, the two fourth staining reagent chambers are symmetrically distributed on both sides of the length direction of the cleaning reagent chamber, the two fifth staining reagent chambers are symmetrically distributed on both sides of the length direction of the cleaning reagent chamber, and the two sixth staining reagent chambers are symmetrically distributed on both sides of the length direction of the cleaning reagent chamber. The first staining reagent chamber, the second staining reagent chamber, the third staining reagent chamber, the fourth staining reagent chamber, the fifth staining reagent chamber and the sixth staining reagent chamber located on the same side of the cleaning reagent chamber are distributed in sequence along a straight line in the width direction of the liquid storage tank.

[0016] The first liquid supply assembly includes two first liquid supply pipes and two first solenoid valves, one end of the two first liquid supply pipes is connected to the two first dyeing reagent chamber outlets, the other end of the two first liquid supply pipes is connected to the two first solenoid valves, and the two first solenoid valves are symmetrically fixed on the bottom of the liquid storage tank. The second liquid supply assembly includes two second liquid supply pipes and two second solenoid valves, one end of the two second liquid supply pipes is connected to the two second dyeing reagent chamber outlets, the other end of the two second liquid supply pipes is connected to the two second solenoid valves, and the two second solenoid valves are symmetrically fixed on the bottom of the liquid storage tank. The third liquid supply assembly includes two third liquid supply pipes and two third solenoid valves, one end of the two third liquid supply pipes is connected to the two third dyeing reagent chamber outlets, the other end of the two third liquid supply pipes is connected to the two third solenoid valves, and the two third solenoid valves are symmetrically fixed on the bottom of the liquid storage tank. The fourth liquid supply assembly includes two fourth liquid supply pipes and two fourth solenoid valves, one end of the two fourth liquid supply pipes is connected to the two fourth dyeing reagent chamber outlets, The second liquid supply assembly comprises two fifth liquid supply pipes and two fifth solenoid valves, one end of the two fifth liquid supply pipes is connected to the liquid outlet of the two fifth dyeing reagent chamber, the other end of the two fifth liquid supply pipes is connected to the two fifth solenoid valves, and the two fifth solenoid valves are symmetrically fixed on the bottom of the liquid storage tank. The third liquid supply assembly comprises two sixth liquid supply pipes and two sixth solenoid valves, one end of the two sixth liquid supply pipes is connected to the liquid outlet of the two sixth dyeing reagent chamber, the other end of the two sixth liquid supply pipes is connected to the two sixth solenoid valves, and the two sixth solenoid valves are symmetrically fixed on the bottom of the liquid storage tank. The fourth liquid supply pipes are connected to the second solenoid valves, the other end of the two fourth solenoid valves is connected to the two fourth solenoid valves, and the two fourth solenoid valves are symmetrically fixed on the bottom of the liquid storage tank. The fifth liquid supply assembly comprises two fifth liquid supply pipes and two fifth solenoid valves, one end of the two fifth liquid supply pipes is connected to the liquid outlet of the two fifth dyeing reagent chamber, the other end of the two sixth liquid supply pipes is connected to the two sixth solenoid valves, and the two sixth solenoid valves are symmetrically fixed on the bottom of the liquid storage tank.

[0017] The liquid storage support frame includes a support platform and four columns. The support platform is square. The bottoms of the four columns are connected to the support base plate. The tops of the four columns are respectively connected to the four corners of the support platform. A through groove is opened in the middle of the support platform. The liquid storage tank is fixed on the support platform. Two first solenoid valves, two second solenoid valves, two third solenoid valves, two fourth solenoid valves, two fifth solenoid valves, two sixth solenoid valves and two seventh solenoid valves all pass through the through groove.

[0018] Compared with the prior art, the beneficial effects and advantages of the present invention are:

[0019] 1. This device abandons the high-throughput structural design of traditional staining machines and takes small volume and low cost as its design concept. While retaining the main structure such as the traditional staining reagent storage chamber, it controls the slide holder to drive the sample to react with different staining reagents to achieve the staining effect. Under the premise of not changing the quality of slide preparation and staining, it reduces the cost and volume of the entire equipment to meet the needs of clinical departments or primary medical institutions.

[0020] 2. The slide holder of the device can meet the forward and backward movement and flip movement control of 2 samples. At the same time, 6 sets of reagent storage chambers are reserved. Different staining requirements can be achieved by operating the solenoid valve. The motion control design simulates human manual operation to improve the operation stability and service life of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the structure of a simple automatic dyeing machine.

[0022] Figure 2 for Figure 1 main view.

[0023] Figure 3 It is a structural schematic diagram of the lateral movement mechanism.

[0024] Figure 4 It is a structural schematic diagram of the flipping mechanism.

[0025] Figure 5 A schematic diagram of the structure of the bracket.

[0026] Figure 6 for Figure 5 main view.

[0027] Figure 7 This is the assembly drawing of the valve control assembly and the fluid storage tank.

[0028] Figure 8 Schematic diagram of the external structure of the liquid storage tank.

[0029] Fig. 9 Schematic diagram of the internal structure of the liquid storage tank.

[0030] Among them, 1-support base plate, 2-base, 3-slide, 4-screw, 5-limit block, 6-first position sensor, 7-second position sensor, 8-flip motor, 9-motor mounting plate, 10-reducer, 11-first bearing seat, 12-second bearing seat, 13-guide rail, 14-slider, 15-film making chamber, 16-bracket, 17-support rod, 18-partition, 19-slide placement slot, 20-stop bar, 21-fixing ear, 22-torsion spring, 23-clamping adjustment shaft, 24-pressing strip, 25-liquid storage tank, 26-cleaning reagent chamber, 27-first staining reagent chamber, 28-second staining reagent chamber, 29-third staining Reagent chamber, 30-fourth staining reagent chamber, 31-fifth staining reagent chamber, 32-first liquid supply pipe, 33-first solenoid valve, 34-second liquid supply pipe, 35-second solenoid valve, 36-third liquid supply pipe, 37-third solenoid valve, 38-fourth liquid supply pipe, 39-fourth solenoid valve, 40-fifth liquid supply pipe, 41-fifth solenoid valve, 42-sixth liquid supply pipe, 43-sixth solenoid valve, 44-support table, 45-column, 46-waste liquid tank, 47-first flip axis, 48-second flip axis, 49-flip support plate, 50-sixth reagent chamber, 51-seventh liquid supply pipe, 52-seventh solenoid valve, 53-connecting support plate. DETAILED DESCRIPTION

[0031] The present invention is described in detail below with reference to the accompanying drawings.

[0032] The structure of the simple automatic dyeing machine provided in this embodiment is as follows Figure 1 and Figure 2 As shown, it includes a supporting base plate, a lateral moving mechanism, a flipping mechanism, a glass slide holder, a liquid storage component, a waste liquid tank, a valve-controlled pipeline and a controller.

[0033] The supporting base plate 1 is square in shape, provides a support and installation basis for the entire dyeing machine, and is located at the bottom of the entire equipment.

[0034] The waste liquid tank 46 is square and is placed in the middle of the supporting base plate 1. The length direction of the waste liquid tank 46 is parallel to the length direction or width direction of the supporting base plate 1. The top of the waste liquid tank 46 is open and is used to hold the waste liquid after dyeing.

[0035] like Figure 3As shown, the lateral movement mechanism is a lateral screw slide, and the lateral movement mechanism includes a screw motor, a base 2, a slide 3, a screw 4, a limit block 5, a first position sensor 6 and a second position sensor 7. The base 2 is fixed on the supporting bottom plate 1, and the screw 4 is parallel to the length direction of the waste liquid tank 46. The first position sensor 6 and the second position sensor 7 are respectively installed on both sides of the base 2, and the limit block 5 is fixed on the bottom of the connecting support plate 53. When the slide 3 moves to the point where the limit block 5 contacts the contact point of the first position sensor 6, the first position sensor 6 transmits a signal to the screw motor, the screw motor stops rotating, and the slide 3 returns to the initial position. When the slide 3 moves to the point where the limit block contacts the contact point of the second position sensor 7, the second position sensor 7 transmits a signal to the screw motor, the screw motor stops rotating, and the slide 3 reaches the terminal position.

[0036] like Figure 4 As shown, the flipping mechanism includes a flipping motor 8, a connecting support plate 53, a motor mounting plate 9, a reducer 10, a first bearing seat 11, a second bearing seat 12, a guide rail 13, a slider 14 and a flipping assembly. The connecting support plate is horizontally fixed to the slide, the motor mounting plate 9 is fixed to the connecting support plate 53, and the motor mounting plate 9 is perpendicular to the connecting support plate 53. The flipping motor 8 and the reducer 10 are both fixedly mounted on the outer side of the motor mounting plate 9, and the output end of the flipping motor 8 is connected to the input end of the reducer 10. The guide rail 13 is mounted on the supporting base plate 1, the guide rail 13 is parallel to the screw 4, and the guide rail 13 and the screw 4 are respectively located on both sides of the waste liquid tank 46, and the slider 14 is mounted on the guide rail 13. The first bearing seat 11 is fixed to the connecting support plate 53, and the second bearing seat 12 is fixed to the slider 14.

[0037] The flip assembly includes a first flip shaft 47, a flip support plate 49, and a second flip shaft 48. The flip support plate 49 is in a square strip shape. The first flip shaft 47 passes through the first bearing seat 11 and the motor mounting plate 9. The first flip shaft 47 is rotatably connected to the motor mounting plate 9. One end of the first flip shaft 47 is connected to the output end of the reducer 10. The second flip shaft 48 passes through the second bearing seat 12. The flip support plate 49 is located between the first bearing seat 11 and the second bearing seat 12. The two ends of the flip support plate 49 are fixedly connected to the other end of the first flip shaft 47 and one end of the second flip shaft 48, respectively. The flip support plate 49 is always located directly above the waste liquid tank 46.

[0038] like Figure 5As shown, the slide holder includes a bracket and two slide making chambers 15, and the bracket includes a bracket 16, a spring clip and a support rod 17. The bracket is quasi-square, and the side wall and the top of the bracket 16 are open in the length direction. There are four support rods 17, and the lower ends of the four support rods 17 are respectively connected to the four corners of the flip support plate 49, and the upper ends of the four support rods 17 are respectively connected to the four corners of the bottom of the bracket 16. A partition 18 is provided in the bracket 16, and the bracket 16 is divided into two symmetrical slide placement grooves 19 along its length direction by the partition. The opening on the side wall of the bracket 16 in the length direction is divided into two slide placement entrances of the slide placement grooves 19 by the partition, and the top openings of the two slide placement grooves 19 are provided with a pressure strip 24.

[0039] When the screw motor rotates, the screw motor drives the slide 3 to move along the length direction of the screw 4, the slide 3 drives the entire flip mechanism to move, the flip mechanism drives the slide holder to move along the length direction of the waste liquid tank 46, thereby driving the two film making chambers 15 to move along the set trajectory. When the flip motor 8 rotates, the flip motor 8 drives the first flip shaft 47 to rotate, the first flip shaft 47 drives the flip support plate 49 to flip, the flip support plate 49 drives the bracket to flip, thereby driving the two film making chambers 15 to flip.

[0040] There are two spring clips, which are respectively matched with the slide insertion ports of the two slide placement slots 19, and the two spring clips are symmetrically arranged. The spring clip includes a clamping adjustment component that can automatically reset and a stop bar 20, and the stop bar 20 is L-shaped. Figure 6 As shown, the clamping adjustment assembly includes two fixing ears 21, a torsion spring 22 and a clamping adjustment shaft 23. The two fixing ears 21 are respectively fixed on the bottom of the bracket 16, and the two fixing ears 21 are respectively located outside the bracket 21, and the two ends of the clamping adjustment shaft 23 are respectively rotatably connected with the two fixing ears 21. The torsion spring 22 is sleeved on the middle part of the clamping adjustment shaft 23, one end of the torsion spring 22 is fixedly connected to the clamping adjustment shaft 23, one end of the stop bar 20 is respectively sleeved on both sides of the clamping adjustment shaft 23, and the other end of the torsion spring 22 is respectively connected to the end of the stop bar 20 sleeved on the clamping adjustment shaft 23. Each clamping adjustment assembly is respectively matched with the corresponding glass slide placement groove 19, and each clamping adjustment assembly is fixed on the bottom of the corresponding glass slide placement groove 19. When each film making chamber 15 is placed on the corresponding glass slide placement groove 19 , the corresponding torsion spring 22 is in a natural state, a straight portion of the corresponding baffle 20 is in close contact with the bottom of the corresponding glass slide placement groove 19 , and the other straight portion clamps the corresponding film making chamber 15 .

[0041] When it is necessary to place the slide clamp of the slide preparation chamber in the slide placement groove 19, press the baffle 20 downward, and then place the slide clamp of the slide preparation chamber 15 into the slide placement groove 19 from the slide placement entrance. After placement, release the baffle 20, and the baffle 20 will be reset under the action of the torsion spring 22. At this time, the top of the slide clamp of the slide preparation chamber 15 is pressed and fixed by the pressure strip 24, and the side of the slide clamp of the slide preparation chamber 15 is clamped and fixed by the baffle 20.

[0042] The liquid storage support frame includes a support platform 44 and four columns 45. The support platform 44 is square. The bottoms of the four columns 45 are connected to the support base plate 1, and the tops of the four columns 45 are respectively connected to the four corners of the support platform 44. A through groove is opened in the middle of the support platform 44.

[0043] The liquid storage tank 25 is square, and the bottom of the liquid storage tank 25 is fixed on the support platform 44. The liquid storage tank 25 is divided into a cleaning reagent chamber 26, two first staining reagent chambers 27, two second staining reagent chambers 28, two third staining reagent chambers 29, two fourth staining reagent chambers 30, two fifth staining reagent chambers 31 and two sixth staining reagent chambers 50. The cleaning reagent chamber 26, the first staining reagent chamber 27, the second staining reagent chamber 28, the third staining reagent chamber 29, the fourth staining reagent chamber 30, the fifth staining reagent chamber 31 and the sixth staining reagent chamber 50 are all square. The symmetry plane of the cleaning reagent chamber 26 coincides with the symmetry plane of the liquid storage tank 25. The top of the cleaning reagent chamber 26 is provided with a liquid inlet, and the bottom of the cleaning reagent chamber 26 is provided with two symmetrically distributed liquid outlets. The two first staining reagent chambers 27 are symmetrically distributed on both sides of the length direction of the cleaning reagent chamber 26. The tops of the two first staining reagent chambers 27 are symmetrically provided with liquid inlets, and the bottoms of the two first staining reagent chambers 27 are symmetrically provided with liquid outlets. The two second staining reagent chambers 28 are symmetrically distributed outside the two sides of the length direction of the cleaning reagent chamber 26, and the tops of the two second staining reagent chambers 28 are symmetrically provided with liquid inlets, and the bottoms of the two second staining reagent chambers 28 are symmetrically provided with liquid outlets. The two third staining reagent chambers 29 are symmetrically distributed outside the two sides of the length direction of the cleaning reagent chamber 26, and the tops of the two third staining reagent chambers 29 are symmetrically provided with liquid inlets, and the bottoms of the two third staining reagent chambers 29 are symmetrically provided with liquid outlets. The two fourth staining reagent chambers 30 are symmetrically distributed outside the two sides of the length direction of the cleaning reagent chamber 26, and the tops of the two fourth staining reagent chambers 30 are symmetrically provided with liquid inlets, and the bottoms of the two fourth staining reagent chambers 30 are symmetrically provided with liquid outlets. The two fifth staining reagent chambers 31 are symmetrically distributed outside the two sides of the length direction of the cleaning reagent chamber 26, and the tops of the two fifth staining reagent chambers 31 are symmetrically provided with liquid inlets, and the bottoms of the two fifth staining reagent chambers 31 are symmetrically provided with liquid outlets. The two sixth staining reagent chambers 50 are symmetrically distributed outside the two sides of the length direction of the cleaning reagent chamber 26, and the tops of the two sixth staining reagent chambers 50 are symmetrically provided with liquid inlets, and the bottoms of the two sixth staining reagent chambers 50 are symmetrically provided with liquid outlets. The first staining reagent chamber 27, the second staining reagent chamber 28, the third staining reagent chamber 29, the fourth staining reagent chamber 30, the fifth staining reagent chamber 31 and the sixth staining reagent chamber 50 located outside the same side of the cleaning reagent chamber 26 are sequentially distributed along a straight line in the width direction of the liquid storage tank 25.

[0044] The valve control assembly includes a first liquid supply assembly, a second liquid supply assembly, a third liquid supply assembly, a fourth liquid supply assembly, a fifth liquid supply assembly and a sixth liquid supply assembly. The first liquid supply assembly includes two first liquid supply pipes 32 and two first solenoid valves 33. One end of the two first liquid supply pipes 32 is connected to the liquid outlets of the two first dyeing reagent chambers 27, respectively. The other end of the two first liquid supply pipes 32 is connected to the two first solenoid valves 33, respectively. The two first solenoid valves 33 are symmetrically fixed on the bottom of the liquid storage tank 25, and the two first solenoid valves 33 pass through the through grooves. The second liquid supply assembly includes two second liquid supply pipes 34 and two second solenoid valves 35. One end of the two second liquid supply pipes 34 is connected to the liquid outlets of the two second dyeing reagent chambers 28, respectively. The other end of the two second liquid supply pipes 34 is connected to the two second solenoid valves 35, respectively. The two second solenoid valves 35 are symmetrically fixed on the bottom of the liquid storage tank 25, and the two second solenoid valves 35 pass through the through grooves. The third liquid supply assembly includes two third liquid supply pipes 36 and two third solenoid valves 37. One end of the two third liquid supply pipes 36 is connected to the liquid outlets of the two third dyeing reagent chambers 29, and the other end of the two third liquid supply pipes 36 is connected to the two second solenoid valves 37. The two third solenoid valves 37 are symmetrically fixed on the bottom of the liquid storage tank 25, and the two third solenoid valves 37 pass through the through slots. The fourth liquid supply assembly includes two fourth liquid supply pipes 38 and two fourth solenoid valves 39. One end of the two fourth liquid supply pipes 38 is connected to the liquid outlets of the two fourth dyeing reagent chambers 30, and the other end of the two fourth liquid supply pipes 38 is connected to the two fourth solenoid valves 39. The two fourth solenoid valves 39 are symmetrically fixed on the bottom of the liquid storage tank 25, and the two fourth solenoid valves 39 pass through the through slots. The fifth liquid supply assembly includes two fifth liquid supply pipes 40 and two fifth solenoid valves 41. One end of the two fifth liquid supply pipes 40 is connected to the liquid outlets of the two fifth dyeing reagent chambers 31, respectively. The other end of the two fifth liquid supply pipes 40 is connected to the two fifth solenoid valves 41, respectively. The two fifth solenoid valves 41 are symmetrically fixed on the bottom of the liquid storage tank 25, and the two fifth solenoid valves 41 pass through the through slots. The sixth liquid supply assembly includes two sixth liquid supply pipes 42 and two sixth solenoid valves 43. One end of the two sixth liquid supply pipes 42 is connected to the liquid outlets of the two sixth dyeing reagent chambers 50, respectively. The other end of the two sixth liquid supply pipes 42 is connected to the two sixth solenoid valves 43, respectively. The two sixth solenoid valves 43 are symmetrically fixed on the bottom of the liquid storage tank 25, and the two sixth solenoid valves 43 pass through the through slots. The seventh liquid supply assembly includes two seventh liquid supply tubes 51 and two seventh solenoid valves 52. One end of the two seventh liquid supply tubes 51 are respectively connected to the two liquid outlets of the cleaning reagent chamber 26, and the other ends of the two seventh liquid supply tubes 51 are respectively connected to the two seventh solenoid valves 52. The two seventh solenoid valves 52 are symmetrically fixed on the bottom of the liquid storage tank 25, and the two seventh solenoid valves 52 respectively pass through the through grooves.

[0045] The first solenoid valve 33, the second solenoid valve 35, the third solenoid valve 37, the fourth solenoid valve 39, the fifth solenoid valve 41, the sixth solenoid valve 43 and the seventh solenoid valve 52 are arranged in a matrix of seven rows and two columns, the two first solenoid valves 33 are located on the first row, the two second solenoid valves 35 are located on the second row, the two third solenoid valves 37 are located on the third row, the two fourth solenoid valves 39 are located on the fourth row, the two fifth solenoid valves 41 are located on the fifth row, the two sixth solenoid valves 43 are located on the sixth row, and the two seventh solenoid valves 52 are located on the seventh row. The two film-making chambers 15 can reciprocate in the horizontal direction driven by the lateral moving mechanism, and the straight lines where the moving trajectories of the two film-making chambers 15 are located are respectively located directly below the straight lines where the columns of the matrix are located.

[0046] The controller is connected to the screw motor, the flip motor 8, the first position sensor 6, the second position sensor 7, the first solenoid valve 33, the second solenoid valve 35, the third solenoid valve 37, the fourth solenoid valve 39, the fifth solenoid valve 41, the sixth solenoid valve 43 and the seventh solenoid valve 52 respectively. The controller automatically controls the working state, working time and working duration of the screw motor, the flip motor 8, the first position sensor 6, the second position sensor 7, the first solenoid valve 33, the second solenoid valve 35, the third solenoid valve 37, the fourth solenoid valve 39, the fifth solenoid valve 41, the sixth solenoid valve 43 and the seventh solenoid valve 52, so that the dyeing machine can automatically dye after the sample to be tested is placed in it.

[0047] The method of using the above-mentioned simple automatic dyeing machine is:

[0048] 1. Put hematoxylin dye solution in the two first dyeing reagent chambers 27, put blue dye solution in the two second dyeing reagent chambers 28, put 95% ethanol solution in the two third dyeing reagent chambers 29; put EA50 dye solution in the two fourth dyeing reagent chambers 30, put anhydrous ethanol in the two fifth dyeing reagent chambers 31, keep the sixth dyeing reagent chamber 50 for standby use, put pure water in the cleaning agent chamber 26, put two samples on two glass slides, put the two glass slides on two slide making chambers 15, and put the two slide making chambers 15 on two glass slide placement grooves 19;

[0049] 2. Turn on the controller, the screw motor, the flip motor 8, the first position sensor 6, the second position sensor 7, the first solenoid valve 33, the second solenoid valve 35, the third solenoid valve 37, the fourth solenoid valve 39, the fifth solenoid valve 41 and the sixth solenoid valve 43 and other electrical components. The controller detects whether the slide 3 is in the initial position. If not, the screw motor works and the slide 3 starts to move until the first position sensor 6 is triggered. The controller receives a signal and the screw motor stops working. At this time, the slide 3 is in the initial position and the two film making chambers 15 are also in the initial position.

[0050] 3. The screw motor works, and the screw motor drives the two film-making chambers 15 to move to a set distance. At this time, the two film-making chambers 15 are respectively located directly below the two seventh solenoid valves 52. The screw motor stops working, and the two seventh solenoid valves 52 are connected. Pure water flows into the two film-making chambers 15 to clean the two samples to be tested. The two seventh solenoid valves 52 are closed after reaching the set working time, and the flip motor 8 starts to rotate. The flip motor 8 drives the two film-making chambers 15 to flip and dump the liquid in the two film-making chambers 15 into the waste liquid tank 46. The flip motor 8 rotates to the preset time and then rotates in the reverse direction. After rotating in the reverse direction for the set time, it stops rotating. At this time, the two film-making chambers 15 are just located directly below the two seventh solenoid valves 52. The screw motor works, and the screw motor drives the two film-making chambers 15 to move to the initial position;

[0051] 4. The screw motor continues to work, and the screw motor drives the two film-making chambers 15 to move to the set distance. At this time, the two film-making chambers 15 are respectively located directly below the two first electromagnetic valves 33, and the screw motor stops working. The two first electromagnetic valves 33 are connected, and the hematoxylin dye solution flows into the two film-making chambers 15 respectively to dye the two samples to be tested. The two first electromagnetic valves 33 are closed after reaching the set working time. After the dyeing time reaches the preset time, the flip motor 8 starts to rotate, and the flip motor 8 drives the two film-making chambers 15 to flip, and dumps the liquid in the two film-making chambers 15 into the waste liquid tank 46. The flip motor 8 rotates to the preset time and then rotates in the reverse direction. After rotating in the reverse direction for the set time, it stops rotating. At this time, the two film-making chambers 15 are just located directly below the two first electromagnetic valves 33, and the screw motor works, and the screw motor drives the two film-making chambers 15 to move to the initial position;

[0052] 5. Repeat step 4 and stain the two samples again with hematoxylin stain;

[0053] 6. Wash the two samples twice with pure water according to the method in step 4;

[0054] 7. Stain the two samples once with blue staining solution according to the method in step 4;

[0055] 8. According to the method in step 4, stain and fix the two samples twice with 95% ethanol solution;

[0056] 9. Stain the two samples twice with EA50 staining solution according to the method in step 4;

[0057] 10. According to the method in step 4, the two samples were stained and fixed three times with 95% ethanol solution;

[0058] 11. According to the method in step 4, stain and fix the two samples twice with anhydrous ethanol.

Claims

1. A simple automatic dyeing machine, characterized in that: It includes a supporting base plate, a lateral moving mechanism, a flipping mechanism, a glass slide holder, a liquid storage component, a waste liquid tank and a valve-controlled pipeline. The fixed part of the lateral moving mechanism is fixed to the supporting base plate, the fixed part of the flipping mechanism is fixed to the moving part of the lateral moving mechanism, the glass slide holder is fixed to the flipping part of the flipping mechanism, the glass slide holder can reciprocate in the horizontal direction, and the glass slide holder can be flipped. The waste liquid tank is open at the top, the waste liquid tank is arranged on the supporting base plate, the glass slide holder is always located directly above the waste liquid tank, the liquid storage component includes a liquid storage tank and a liquid storage support frame, the liquid storage support frame is fixed to the supporting base plate, the liquid storage tank is fixed to the liquid storage support frame, and the liquid storage tank is divided into a first staining reagent storage unit, a second staining reagent storage unit ... an Nth staining reagent storage unit and a cleaning reagent storage unit; The valve control assembly includes a first liquid supply assembly...an Nth liquid supply assembly and an N+1th liquid supply assembly, the first liquid supply assembly includes a first liquid supply pipeline and a first electromagnetic valve group, the first electromagnetic valve group includes a plurality of first electromagnetic valves distributed along a straight line perpendicular to the moving direction of the glass slide holder, the first liquid supply pipeline is connected to the liquid outlet of the first staining reagent unit, the first liquid supply pipeline is respectively connected to the plurality of first electromagnetic valves...the Nth electromagnetic valve group includes a plurality of Nth electromagnetic valves distributed along a straight line perpendicular to the moving direction of the glass slide holder, the Nth liquid supply pipeline is connected to the Nth staining reagent unit. The liquid outlet is connected, the Nth liquid supply pipeline is respectively connected to a plurality of Nth solenoid valves, the N+1th solenoid valve group includes a plurality of N+1th solenoid valves distributed along a straight line perpendicular to the moving direction of the glass slide holder, the N+1th liquid supply pipeline is connected to the liquid outlet of the cleaning reagent storage unit, and the N+1th liquid supply pipeline is respectively connected to a plurality of N+1th solenoid valves; the first solenoid valve group...the Nth solenoid valve group and the N+1th solenoid valve group are distributed along a straight line parallel to the moving direction of the glass slide holder, and the first solenoid valve...the Nth solenoid valve and the N+1th solenoid valve are distributed in a matrix; The slide holder is located below the first solenoid valve group ... the Nth solenoid valve group and the N+1th solenoid valve group, and a plurality of slide positions for mounting slides are provided on the slide holder, and the number of slide positions, the first solenoid valve ... the Nth solenoid valve and the N+1th solenoid valve is equal, and the straight lines where the moving tracks of the plurality of slide positions are located are respectively located directly below the straight lines where the rows or columns of the matrix are located; The lateral movement mechanism is a lateral screw slide, which includes a base, a slide, a screw, a limit block, a first position sensor for sensing that the slide returns to an initial position, and a second position sensor for sensing that the slide reaches an end position. The base is fixed to a supporting bottom plate, the first position sensor and the second position sensor are respectively mounted on both sides of the base, and the limit block is mounted on the slide, and the limit block can trigger the first position sensor and the second position sensor respectively. The flip mechanism comprises a flip motor and a flip assembly. The flip motor is fixed on the slide, the flip transmission assembly is connected to the output end of the flip motor, and the glass slide holder is installed on the flip assembly.

2. The simple automatic dyeing machine according to claim 1, characterized in that: The flipping mechanism also includes a motor mounting plate, a connecting support plate, a reducer, a first bearing seat, a second bearing seat, a guide rail and a slider. The connecting support plate is horizontally fixed to the slide, the motor mounting plate is fixed to the connecting support plate, the motor mounting plate is perpendicular to the connecting support plate, the flipping motor and the reducer are both fixedly mounted on the outer side of the motor mounting plate, the output end of the flipping motor is connected to the input end of the reducer, the guide rail is mounted on the supporting bottom plate, the guide rail is parallel to the screw, and the guide rail and the screw are respectively located on both sides of the waste liquid tank, the slider is mounted on the guide rail, the first bearing seat is fixed to the connecting support plate, the second bearing is fixed to the slider, the flipping assembly includes a first flipping axis, a flipping support plate and a second flipping axis, the first flipping axis passes through the first bearing seat and the motor mounting plate, the first flipping axis is rotatably connected to the motor mounting plate, one end of the first flipping axis is connected to the output end of the reducer, the second flipping axis passes through the second bearing seat, the flipping support plate is located between the first bearing seat and the second bearing seat, and the flipping support plate is respectively connected to the other end of the first flipping axis and one end of the second flipping axis.

3. The simple automatic dyeing machine according to claim 2, characterized in that: The glass slide holder comprises a bracket and a film making chamber, the bracket comprises a bracket, a spring clip and a support rod, the bracket is square-shaped, there are multiple support rods, the multiple support rods are evenly distributed between the bracket and the flip support plate, the lower end of each support rod is fixed to the flip support plate, the upper end of each support rod is fixed to the bottom of the bracket, the side walls and the top of the bracket in a length direction are open, a plurality of evenly distributed partitions are arranged in the bracket along its length direction, the bracket is divided into a plurality of glass slide placement grooves by the partitions, the openings on the side walls in the length direction of the bracket are divided into a plurality of glass slide placement grooves by the partitions, a pressure strip is arranged on the top opening of each glass slide placement groove, the number of the spring clips is equal to the number of the glass slide placement grooves, and each spring clip cooperates with the glass slide placement entrance of the corresponding glass slide placement groove.

4. The simple automatic dyeing machine according to claim 3 is characterized in that: The spring clamp comprises a clamping adjustment component and a baffle that can automatically reset. The clamping adjustment component comprises two fixing ears, a torsion spring and a clamping adjustment shaft. The two fixing ears are respectively fixed on the bottom of the bracket groove, and the two fixing ears are respectively located outside the bracket. The two ends of the clamping adjustment shaft are respectively rotatably connected with the two fixing ears. The torsion spring is sleeved on the middle part of the clamping adjustment shaft. One end of the torsion spring is fixedly connected with the clamping adjustment shaft. The baffle is L-shaped. One end of the baffle is respectively sleeved on both sides of the clamping adjustment shaft. The other end of the torsion spring is respectively sleeved with the baffle on the clamping The ends of the adjusting shaft are connected, and each clamping and adjusting assembly cooperates with the corresponding glass slide placement groove, and each clamping and adjusting assembly is fixed on the bottom of the corresponding glass slide placement groove. There are multiple film making chambers, and the number of film making chambers is equal to the number of glass slide placement grooves. When each film making chamber is placed on the corresponding glass slide placement groove, the corresponding torsion spring is in a natural state, a straight part of the corresponding baffle is close to the bottom of the corresponding glass slide placement groove, and the other straight part clamps the corresponding film making chamber, and each glass slide placement groove and the corresponding film making chamber constitute a glass slide position.

5. The simple automatic dyeing machine according to claim 3, characterized in that: There are two slide placement slots, and the liquid storage box is square. The liquid storage box is divided into a cleaning reagent chamber, two first staining reagent chambers, two second staining reagent chambers, two third staining reagent chambers, two fourth staining reagent chambers, two fifth staining reagent chambers and a sixth staining reagent chamber. The cleaning reagent chamber, the first staining reagent chamber, the second staining reagent chamber, the third staining reagent chamber, the fourth staining reagent chamber, the fifth staining reagent chamber and the sixth staining reagent chamber are all square. The symmetry plane of the cleaning reagent chamber coincides with the symmetry plane of the liquid storage box. The two first staining reagent chambers are symmetrically distributed on both sides of the length direction of the cleaning reagent chamber. The two second staining reagent chambers are symmetrically distributed on both sides of the length direction of the cleaning reagent chamber. The two third staining reagent chambers are symmetrically distributed on both sides of the length direction of the cleaning reagent chamber, the two fourth staining reagent chambers are symmetrically distributed on both sides of the length direction of the cleaning reagent chamber, the two fifth staining reagent chambers are symmetrically distributed on both sides of the length direction of the cleaning reagent chamber, and the two sixth staining reagent chambers are symmetrically distributed on both sides of the length direction of the cleaning reagent chamber. The first staining reagent chamber, the second staining reagent chamber, the third staining reagent chamber, the fourth staining reagent chamber, the fifth staining reagent chamber and the sixth staining reagent chamber located on the same side of the cleaning reagent chamber are distributed in sequence along a straight line in the width direction of the liquid storage tank.

6. The simple automatic dyeing machine according to claim 5, characterized in that: The first liquid supply assembly includes two first liquid supply pipes and two first solenoid valves, one end of the two first liquid supply pipes is connected to the two first dyeing reagent chamber outlets, the other end of the two first liquid supply pipes is connected to the two first solenoid valves, and the two first solenoid valves are symmetrically fixed on the bottom of the liquid storage tank. The second liquid supply assembly includes two second liquid supply pipes and two second solenoid valves, one end of the two second liquid supply pipes is connected to the two second dyeing reagent chamber outlets, the other end of the two second liquid supply pipes is connected to the two second solenoid valves, and the two second solenoid valves are symmetrically fixed on the bottom of the liquid storage tank. The third liquid supply assembly includes two third liquid supply pipes and two third solenoid valves, one end of the two third liquid supply pipes is connected to the two third dyeing reagent chamber outlets, the other end of the two third liquid supply pipes is connected to the two third solenoid valves, and the two third solenoid valves are symmetrically fixed on the bottom of the liquid storage tank. The fourth liquid supply assembly includes two fourth liquid supply pipes and two fourth solenoid valves, one end of the two fourth liquid supply pipes is connected to the two fourth dyeing reagent chamber outlets, The second liquid supply assembly comprises two fifth liquid supply pipes and two fifth solenoid valves, one end of the two fifth liquid supply pipes is connected to the liquid outlet of the two fifth dyeing reagent chamber, the other end of the two fifth liquid supply pipes is connected to the two fifth solenoid valves, and the two fifth solenoid valves are symmetrically fixed on the bottom of the liquid storage tank. The third liquid supply assembly comprises two sixth liquid supply pipes and two sixth solenoid valves, one end of the two sixth liquid supply pipes is connected to the liquid outlet of the two sixth dyeing reagent chamber, the other end of the two sixth liquid supply pipes is connected to the two sixth solenoid valves, and the two sixth solenoid valves are symmetrically fixed on the bottom of the liquid storage tank. The fourth liquid supply pipes are connected to the second solenoid valves, the other end of the two fourth solenoid valves is connected to the two fourth solenoid valves, and the two fourth solenoid valves are symmetrically fixed on the bottom of the liquid storage tank. The fifth liquid supply assembly comprises two fifth liquid supply pipes and two fifth solenoid valves, one end of the two fifth liquid supply pipes is connected to the liquid outlet of the two fifth dyeing reagent chamber, the other end of the two sixth liquid supply pipes is connected to the two sixth solenoid valves, and the two sixth solenoid valves are symmetrically fixed on the bottom of the liquid storage tank.

7. The simple automatic dyeing machine according to claim 5, characterized in that: The liquid storage support frame includes a support platform and four columns. The support platform is square. The bottoms of the four columns are connected to the support base plate. The tops of the four columns are respectively connected to the four corners of the support platform. A through groove is opened in the middle of the support platform. The liquid storage tank is fixed on the support platform. Two first solenoid valves, two second solenoid valves, two third solenoid valves, two fourth solenoid valves, two fifth solenoid valves, two sixth solenoid valves and two seventh solenoid valves all pass through the through groove.

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