SNP (Single Nucleotide Polymorphism) molecular marker related to sika deer antler weight character as well as detection primer, kit and application of SNP molecular marker

A molecular marker, sika velvet technology, applied in the field of molecular genetics, can solve the problems of less research on related traits, achieve early screening and prediction, improve accuracy and efficiency, and improve breeding efficiency

CN114231644APending Publication Date: 2022-03-25INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2022-03-25

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Abstract

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to a sika deer antler weight character as well as a detection primer, a kit and application thereof, and relates to the technical field of molecular genetics. The SNP molecular marker is located on an MSRB3 gene, T / C base mutation exists at the g.44455582 site, and the polymorphism of the site has significant correlation with the weight character of sika deer antler. According to the invention, early prediction and screening of sika deer can be realized by detecting the SNP site, sika deer with high antler weight and sika deer with low antler weight can be distinguished, and breeding of sika deer with high antler yield can be assisted.
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Description

technical field

[0001] The invention relates to the technical field of molecular genetics, in particular to a SNP molecular marker related to the heavy trait of sika antler, detection primers, a kit and application thereof. Background technique

[0002] Methionine sulfoxide reductase B3 (methionine sulfoxide reductase B3, MSRB3) gene is a member of the MSRB gene family and is an oxidoreductase that can specifically catalyze the reduction of R-methionine sulfoxide to methionine ( Kwak GH et al., 2017). MSRB3 is a pleiotropic gene that can encode two types of proteins, MSRB3A and MSRB3B, in humans. The former is located in the endoplasmic reticulum and the latter is located in the mitochondria (Kim HY et al., 2004). MSRB3 plays a key role in aging and stress tolerance, and Drosophila lines overexpressing the human MSRB3 gene exhibited longer lifespan and increased resistance to oxidative, cold and heat stress (D.H.Lim et al. 2012 and G.H.Kwak et al. 2012). The MSRB3 gene re...

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Embodiment

[0046] 1. Materials and methods

[0047] 1.1 Test animals

[0048] A total of 314 sika deer males at the age of 24 months with the same feeding conditions were selected as research objects in Dongdalu Farm, Changchun, Jilin. After venous blood was collected, it was anticoagulated with ethylenediamine tetraacetic acid (EDTA) and frozen for future use. Weigh the weight of Ergang antler at about 45 days of growth and record the data.

[0049] 1.2 Main reagents

[0050] Blood DNA Extraction Kit, FastPfu PCR SuperMix (-dye) was purchased from Beijing Quanshijin Biotechnology Co., Ltd.; 2×Es Taq MasterMix (Dye) was purchased from Beijing Kangwei Century Biotechnology Co., Ltd.; DNA Marker, 6×Loading Buffer, 10×Loading Buffer All were purchased from TaKaRa Company.

[0051] 1.3 DNA extraction and primer synthesis

[0052] Referring to the MSRB3 gene sequence in the sika deer genome, primers were designed using Primer-BLAST in NCBI. The amplified product contained all exons and...