A culture device for preparing large intestine cell extract and a method of using the same

Abstract

The present invention relates to the technical field of culture devices, and in particular to a culture device for preparing a large intestine cell extract and a method for using the culture device, comprising a culture device box, a support seat being slidably arranged in the culture device box, a circular plate being arranged above the support seat, a receiving groove being provided in the middle of the upper surface of the circular plate, a cylinder being movably inserted in the receiving groove, and the downward movement of a connecting block will drive a fixing rod and a support rod to move downward, so that a second round cover moves downward, and the movement of the second round cover will drive a stirring rod and a stirring rod to be inserted into the cylinder, and at this time, the second round cover is started to rotate the stirring rod, and the rotation of the stirring rod will drive the stirring rod to rotate, so that the large intestine cells and culture medium in the cylinder are stirred and mixed, thereby accelerating the reaction efficiency and thus accelerating the speed of preparing the large intestine cell extract.

Description

Technical Field

[0001] The present invention relates to the technical field of culture devices, and in particular to a culture device for preparing a large intestine cell extract and a use method thereof. Background Art

[0002] Cell culture technology refers to the growth of cells under in vitro conditions. During the culture process, cells no longer form tissues (animals), and the cultured products are single cells or cell groups. Cells must live in an artificial environment during culture. Due to changes in the environment, cell movement or the influence of some other factors, the culture time is prolonged and the cells are passaged, resulting in a single type.

[0003] For example, a Chinese patent discloses: a culture device, publication number: CN107406813A, "In the culture device, compared with the contact between the shelf and the same raw material, the friction coefficient is reduced, the shelf is easy to slide, and it is easy to pull out and put in, which can make the sliding of the shelf easy", but in actual use, it is not convenient to stir and mix the large intestine cells and culture medium in the cylinder, and it is not convenient to centrifuge the large intestine cells after the reaction is completed. The operation is relatively cumbersome, which is not convenient for the subsequent preparation of large intestine cell extracts, and reduces the efficiency of preparing large intestine cell extracts. Summary of the invention

[0004] In order to overcome the deficiencies of the prior art, the present invention provides a culture device for preparing a large intestine cell extract and a method for using the same.

[0005] To solve the above technical problems, the present invention provides the following technical solutions: a culture device for preparing colon cell extracts, comprising a culture device box, a support seat is slidably arranged in the culture device box, a circular plate is arranged above the support seat, a receiving groove is provided in the middle of the upper surface of the circular plate, a cylinder is movably inserted in the receiving groove, a rotating structure for driving the cylinder to rotate is provided in the culture device box, the rotating structure comprises a motor 1, a cylinder and a first round cover, the motor 1 is fixedly mounted on the upper end of the support seat, an output shaft 1 of the support seat is fixedly connected to the middle of the bottom end of the circular plate, both side ends of the cylinder are fixedly connected with plug rods, both sides of the upper surface of the circular plate are provided with positioning grooves, the bottom ends of the two plug rods are movably inserted in the positioning grooves respectively, the cylinder is fixedly mounted on the upper inner wall of the culture device box, a telescopic rod is provided on the cylinder, the bottom end of the first round cover is movably inserted in the cylinder, the middle of the first round cover A bearing 1 is fixedly installed, and the telescopic rod is rotatably connected to the first round cover through the bearing 1. The two side ends of the first round cover are fixedly connected with positioning blocks, and the two positioning blocks are respectively movably sleeved on the upper ends of the two insertion rods. A stirring structure is arranged in the culture device box, and the stirring structure includes a second round cover, a stirring rod and a connecting block. A driving structure for driving the support seat to move is arranged on the culture device box, and the driving structure includes a motor 3. A slide groove 3 is provided on the bottom surface of the culture device box, and the two side ends of the support seat are fixedly connected with connecting rods. Three round rods are fixedly installed on the upper end of the support seat, and the three round rods are distributed in a circle. A sliding door is provided on the culture device box, and the inner wall surfaces on both sides of the culture device box are provided with slide grooves 1 in the horizontal direction. The two connecting rods are respectively slidably arranged in the slide grooves 1, and the inner wall bottom surface of the culture device box is fixedly connected with a sliding rod, and the end of the sliding rod is fixedly connected with a baffle.

[0006] As a preferred technical solution of the present invention, the baffle plate movably passes through the slide groove three, and an annular groove is provided on the bottom surface of the circular plate.

[0007] As a preferred technical solution of the present invention, a through opening is provided on the upper surface of the culture device box, a circular ring communicating with the through opening is fixedly installed on the upper end of the culture device box, a fan is arranged inside the circular ring, a removable dustproof net is installed on the upper end of the circular ring, and two inner wall surfaces on both sides of the culture device box are provided with sliding grooves in the vertical direction, and the sliding grooves are slidably connected to the corresponding rectangular rods.

[0008] As a preferred technical solution of the present invention, a second motor is fixedly installed on the upper end of the second round cover, a second bearing is fixedly installed on the middle part of the second round cover, the stirring rod movably passes through the second round cover, and is rotatably connected to the second round cover through the second bearing.

[0009] As a preferred technical solution of the present invention, a stirring rod is fixedly installed on the stirring rod, the connecting block is fixedly installed on the telescopic rod, the end of the connecting block is fixedly connected to a fixing rod, and the connecting block is fixedly connected to the second round cover through the fixing rod.

[0010] As a preferred technical solution of the present invention, both side ends of the connecting block are fixedly connected with support rods, both side ends of the second round cover are fixedly connected with rectangular rods, the support rods are fixedly connected to the corresponding rectangular rods, and the fixed rod is located between the two support rods.

[0011] As a preferred technical solution of the present invention, the motor three is fixedly installed at the end of the culture device box, the output shaft three of the motor three is fixedly connected with a threaded rod, the threaded rod is rotatably installed in the culture device box, the threaded rod passes through the support seat, and is threadedly connected to the support seat.

[0012] A method for using a culture device for preparing a large intestine cell extract comprises the following steps:

[0013] S1: Pour large intestinal cells and a certain amount of culture medium into the cylinder;

[0014] S2: The stirring rod is driven to rotate by the rotation of the stirring rod, so as to stir and mix the large intestine cells and the culture medium in the cylinder;

[0015] S3: After the reaction between the large intestine cells and the culture medium in the cylinder is completed, the motor 1 is started to rotate the circular plate. The rotation of the circular plate drives the cylinder and the first circular cover to rotate, and the large intestine cells in the cylinder that have completed the reaction are centrifuged.

[0016] Compared with the prior art, the present invention can achieve the following beneficial effects:

[0017] First, open the sliding door, pour the colon cells and a certain amount of culture medium into the cylinder, start the cylinder, and move the telescopic rod downward. The movement of the telescopic rod will drive the connecting block to move downward. The downward movement of the connecting block will drive the fixed rod and the support rod to move downward, so that the second round cover moves downward. The movement of the second round cover will drive the stirring rod and the stirring rod to be inserted into the cylinder. At this time, start the second round cover to rotate the stirring rod. The rotation of the stirring rod will drive the stirring rod to rotate, and the colon cells and culture medium in the cylinder will be stirred and mixed, which will speed up the reaction efficiency and thus speed up the preparation of colon cell extracts.

[0018] Secondly, after the reaction of the large intestine cells and culture medium in the cylinder is completed, start motor three to rotate the threaded rod, and the rotation of the threaded rod will drive the support seat to move, so that the support seat moves towards the user, and the movement of the support seat will drive the circular plate and the cylinder to move, so that the cylinder is directly below the first round cover, start the cylinder to move the telescopic rod downward, and the movement of the telescopic rod will drive the first round cover to move downward, so that the bottom end of the first round cover is inserted into the cylinder, and the two positioning blocks are respectively movably sleeved on the insertion rod, so that the connection between the first round cover and the cylinder is completed, start motor one to rotate the circular plate, and the rotation of the circular plate will drive the cylinder and the first round cover to rotate, and the large intestine cells after the reaction in the cylinder are centrifuged, which is convenient for the subsequent preparation of large intestine cell extracts, improves the preparation efficiency, and is more convenient to operate.

[0019] Thirdly, the first round cover is movably inserted in the cylinder, and the two positioning blocks are movably sleeved on the insertion rod, so that the cylinder is connected to the circular plate, so that the rotation of the circular plate can drive the cylinder to rotate, thereby centrifuging the colon cells that have completed the reaction in the cylinder. Furthermore, the first round cover plays a blocking role, preventing the colon cells in the cylinder from being thrown out during the rotation of the cylinder, thereby ensuring the stable operation of the centrifugal treatment of the colon cells. By providing the second round cover, during the stirring and mixing process of the colon cells and the culture medium in the cylinder, the splashing of the colon cells and the culture medium can be effectively avoided, thereby ensuring the stable operation of the stirring structure.

[0020] Fourthly, a fan is arranged inside the ring, so that the air inside the culture device box and the outside air can circulate, which can provide an environment conducive to the cultivation of large intestine cells. A dustproof net is installed on the ring to prevent external dust and other impurities from entering the culture device box, thereby effectively avoiding the contamination of large intestine cells, which is conducive to the cultivation of large intestine cells and improves the practicality of the culture device for preparing large intestine cell extracts.

[0021] Fifth, by providing a driving structure, the rotation of the threaded rod will drive the support seat to move, and the movement of the support seat can drive the cylinder to move, thereby making it convenient for the user to place or take out the cylinder, making the operation more convenient and more convenient to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic structural diagram of the culture device for preparing large intestine cell extract of the present invention;

[0023] Figure 2 For the present invention Figure 1 The enlarged structural diagram at A in the middle;

[0024] Figure 3 It is a schematic diagram of the structure of the ring of the present invention;

[0025] Figure 4 It is a schematic diagram of the structure of the culture device box body cut away in the present invention;

[0026] Figure 5 It is a structural schematic diagram of the support base of the present invention;

[0027] Figure 6 It is a structural schematic diagram of the circular plate of the present invention;

[0028] Figure 7 It is a schematic structural diagram of the cylinder of the present invention.

[0029] Among them: 1. Culture device box; 11. Sliding door; 12. Slide slot 1; 13. Slide slot 2; 14. Through hole; 15. Slide rod; 16. Baffle; 17. Ring; 18. Dust net; 2. Support seat; 21. Slide slot 3; 22. Connecting rod; 23. Round plate; 24. Accommodating groove; 25. Cylinder; 26. Insert rod; 27. Annular groove; 28. Positioning groove; 29. ​​Round rod; 3. Motor 1; 4. Cylinder; 41. Telescopic rod; 5. First round cover; 51. Bearing 1; 52. Positioning block; 6. Second round cover; 61. Motor 2; 62. Bearing 2; 63. Rectangular rod; 7. Stirring rod; 71. Stirring rod; 8. Connecting block; 81. Fixed rod; 82. Support rod; 9. Motor 3; 91. Threaded rod. DETAILED DESCRIPTION

[0030] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific embodiments, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the embodiments in the implementation mode, other embodiments obtained by those skilled in the art without creative work all belong to the protection scope of the present invention. The experimental methods in the following embodiments, unless otherwise specified, are conventional methods, and the materials, reagents, etc. used in the following embodiments, unless otherwise specified, can all be obtained from commercial channels.

[0031] like Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 and Figure 7As shown, a culture device for preparing a large intestine cell extract and a method of using the same include a culture device housing 1, a support seat 2 is slidably arranged in the culture device housing 1, a circular plate 23 is arranged above the support seat 2, a receiving groove 24 is opened in the middle of the upper surface of the circular plate 23, a cylinder 25 is movably inserted in the receiving groove 24, a rotating structure for driving the cylinder 25 to rotate is arranged in the culture device housing 1, and the rotating structure includes a motor 3, a cylinder 4 and a first circular cover 5, the motor 3 is fixedly installed on the upper end of the support seat 2, the output shaft 1 of the support seat 2 is fixedly connected to the middle of the bottom end of the circular plate 23, and the two ends of the cylinder 25 are fixedly connected. The side ends are fixedly connected with the plug rod 26, and the two sides of the upper surface of the circular plate 23 are provided with positioning grooves 28. The bottom ends of the two plug rods 26 are respectively movably inserted in the positioning grooves 28. The cylinder 4 is fixedly installed on the upper inner wall of the culture device box 1. The cylinder 4 is provided with a telescopic rod 41. The bottom end of the first round cover 5 is movably inserted in the cylinder 25. A bearing 51 is fixedly installed in the middle of the first round cover 5. The telescopic rod 41 is rotatably connected to the first round cover 5 through the bearing 51. The two side ends of the first round cover 5 are fixedly connected with positioning blocks 52. The two positioning blocks 52 are respectively movably sleeved on the upper ends of the two plug rods 26. The culture device box 1 is provided with a telescopic rod 41. The bottom end of the first round cover 5 is movably inserted in the cylinder 25. The middle part of the first round cover 5 is fixedly installed with a bearing 51. The telescopic rod 41 is rotatably connected to the first round cover 5 through the bearing 51. The two side ends of the first round cover 5 are fixedly connected with positioning blocks 52. The two positioning blocks 52 are respectively movably sleeved on the upper ends of the two plug rods 26. A stirring structure is arranged in the device box 1, and the stirring structure includes a second round cover 6, a stirring rod 7 and a connecting block 8. A driving structure for driving the support seat 2 to move is arranged on the culture device box 1, and the driving structure includes a motor 3 9. A slide groove 3 21 is provided on the bottom surface of the culture device box 1, and both side ends of the support seat 2 are fixedly connected with connecting rods 22. Three round rods 29 are fixedly installed on the upper end of the support seat 2, and the three round rods 29 are distributed in a circle. A sliding door 11 is arranged on the culture device box 1, and both side inner wall surfaces of the culture device box 1 are provided with slide grooves 12 in the horizontal direction, and the two connecting rods 22 slide respectively. The culture device box 1 is arranged in the slide groove 12, the inner wall bottom surface of the culture device box 1 is fixedly connected with a slide rod 15, the end of the slide rod 15 is fixedly connected with a baffle 16, the baffle 16 movably passes through the slide groove 3 21, the bottom surface of the circular plate 23 is provided with an annular groove 27, the upper surface of the culture device box 1 is provided with a through opening 14, the upper end of the culture device box 1 is fixedly installed with a ring 17 connected with the through opening 14, a fan is arranged in the ring 17, and a detachable dustproof net 18 is installed on the upper end of the ring 17, and the inner wall surfaces on both sides of the culture device box 1 are provided with slide grooves 2 13 in the vertical direction, and the slide grooves 2 are slidably connected with the corresponding rectangular rods;A second motor 61 is fixedly installed on the upper end of the second round cover 6, a second bearing 62 is fixedly installed on the middle part of the second round cover 6, the stirring rod 7 movably penetrates the second round cover 6, and is rotatably connected to the second round cover 6 through the second bearing 62, a stirring rod 71 is fixedly installed on the stirring rod 7, the connecting block 8 is fixedly installed on the telescopic rod 41, and the end of the connecting block 8 is fixedly connected to the fixing rod 81, the sliding door 11 is opened, the large intestine cells and a certain amount of culture medium are poured into the cylinder 25, the cylinder 4 is started, and the telescopic rod 41 moves downward, the movement of the telescopic rod 41 drives the connecting block 8 to move downward, and the downward movement of the connecting block 8 drives the fixing rod 81 and the support rod 8 2 moves downward, so that the second round cover 6 moves downward, and the movement of the second round cover 6 drives the stirring rod 7 and the stirring rod 71 to be inserted into the cylinder 25. At this time, the second round cover 6 is started, so that the stirring rod 7 rotates, and the rotation of the stirring rod 7 drives the stirring rod 71 to rotate, so that the large intestine cells and the culture medium in the cylinder 25 are stirred and mixed, which speeds up the reaction efficiency, thereby speeding up the preparation of the large intestine cell extract. After the large intestine cells and the culture medium in the cylinder 25 have completed the reaction, the motor 3 9 is started to rotate the threaded rod 91, and the rotation of the threaded rod 91 drives the support seat 2 to move, so that the support seat 2 is moved closer to the cylinder 25. The cylinder 25 is moved in the direction of the user, and the movement of the support seat 2 drives the circular plate 23 and the cylinder 25 to move, so that the cylinder 25 is directly below the first circular cover 5. The cylinder 4 is started to move the telescopic rod 41 downward, and the movement of the telescopic rod 41 drives the first circular cover 5 downward, so that the bottom end of the first circular cover 5 is inserted into the cylinder 25, so that the two positioning blocks 52 are respectively movably sleeved on the insertion rod 26, so that the connection between the first circular cover 5 and the cylinder 25 is completed, and the motor 3 is started to rotate the circular plate 23, and the rotation of the circular plate 23 drives the cylinder 25 and the first circular cover 5 to rotate, so that the large intestine cells in the cylinder 25 after the reaction is completed are Centrifugal treatment is convenient for subsequent preparation of colon cell extract, improves preparation efficiency, and is more convenient to operate. The connecting block 8 is fixedly connected to the second round cover 6 through a fixing rod 81. The two side ends of the connecting block 8 are fixedly connected with support rods 82. The two side ends of the second round cover 6 are fixedly connected with rectangular rods 63. The support rods 82 are fixedly connected with the corresponding rectangular rods 63. The fixing rod 81 is between the two support rods 82. The motor 3 9 is fixedly installed at the end of the culture device box 1. The output shaft 3 of the motor 3 9 is fixedly connected with a threaded rod 91. The threaded rod 91 is rotatably installed in the culture device box 1. The threaded rod 91 passes through the support seat 2 and is threadedly connected with the support seat 2. ;

[0032] When in use, the sliding door 11 is opened, and the large intestine cells and a certain amount of culture medium are poured into the barrel 25, and the cylinder 4 is started to make the telescopic rod 41 move downward, and the movement of the telescopic rod 41 will drive the connecting block 8 to move downward, and the downward movement of the connecting block 8 will drive the fixing rod 81 and the supporting rod 82 to move downward, so that the second round cover 6 moves downward, and the movement of the second round cover 6 will drive the stirring rod 7 and the stirring rod 71 to be inserted into the barrel 25. At this time, the second round cover 6 is started to make the stirring rod 7 rotate, and the rotation of the stirring rod 7 will drive the stirring rod 71 to rotate, so that the large intestine cells and the culture medium in the barrel 25 are stirred and mixed, which speeds up the reaction efficiency, thereby speeding up the preparation of the large intestine cell extract;

[0033] After the reaction of the large intestine cells and the culture medium in the cylinder 25 is completed, the motor 3 9 is started to rotate the threaded rod 91. The rotation of the threaded rod 91 drives the support seat 2 to move, so that the support seat 2 moves in the direction close to the user. The movement of the support seat 2 drives the circular plate 23 and the cylinder 25 to move, so that the cylinder 25 is directly below the first round cover 5. The cylinder 4 is started to move the telescopic rod 41 downward. The movement of the telescopic rod 41 drives the first round cover 5 to move downward, so that the bottom end of the first round cover 5 is inserted into the cylinder 25, so that the two positioning blocks 52 are respectively movably sleeved on the insertion rod 26, so that the connection between the first round cover 5 and the cylinder 25 is completed. The motor 1 3 is started to rotate the circular plate 23. The rotation of the circular plate 23 drives the cylinder 25 and the first round cover 5 to rotate, and the large intestine cells in the cylinder 25 after the reaction are completed are centrifuged, which is convenient for the subsequent preparation of large intestine cell extracts, improves the preparation efficiency, and is more convenient to operate.

[0034] The first round cover 5 is movably inserted into the cylinder 25, and the two positioning blocks 52 are movably sleeved on the insertion rod 26, so that the cylinder 25 is connected to the circular plate 23, so that the rotation of the circular plate 23 can drive the cylinder 25 to rotate, so that the colon cells in the cylinder 25 that have been reacted can be centrifuged. Further, the first round cover 5 plays a blocking role, preventing the colon cells in the cylinder 25 from being thrown out during the rotation of the cylinder 25, thereby ensuring the stable operation of the centrifugal treatment of the colon cells. By providing the second round cover 6, in the process of stirring and mixing the colon cells and the culture medium in the cylinder 25, the colon cells and the culture medium can be effectively prevented from splashing out, thereby ensuring the stable operation of the stirring structure;

[0035] The fan is arranged in the ring 17, so that the air in the culture device box 1 and the outside air can be circulated, which can provide an environment conducive to the cultivation of large intestine cells. The dustproof net 18 is installed on the ring 17 to prevent the outside dust and other impurities from entering the culture device box 1, so that the large intestine cells can be effectively prevented from being contaminated, which is conducive to the cultivation of large intestine cells and improves the practicality of the culture device for preparing large intestine cell extracts.

[0036] By providing a driving structure, the rotation of the threaded rod 91 will drive the support base 2 to move, and the movement of the support base 2 can drive the cylinder 25 to move, so that it is convenient for the user to place or remove the cylinder 25, and the operation is more convenient and the use is more convenient.

[0037] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited thereto, and various changes can be made within the knowledge scope of technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. A culture device for preparing a large intestine cell extract, comprising a culture device housing (1), characterized in that: A support seat (2) is slidably arranged in the culture device box (1), a circular plate (23) is arranged above the support seat (2), a receiving groove (24) is provided in the middle of the upper surface of the circular plate (23), a barrel (25) is movably inserted in the receiving groove (24), a rotating structure for driving the barrel (25) to rotate is arranged in the culture device box (1), the rotating structure comprises a motor (3), a cylinder (4) and a first circular cover (5), the motor (3) is fixedly mounted on the upper end of the support seat (2), an output shaft (1) of the support seat (2) is fixedly connected to the middle of the bottom end of the circular plate (23), both side ends of the barrel (25) are fixedly connected with plug rods (26), both sides of the upper surface of the circular plate (23) are provided with positioning grooves (28), and the bottom ends of the two plug rods (26) are respectively movably inserted in the positioning grooves (28). The cylinder (4) is fixedly mounted on the upper inner wall surface of the culture device housing (1), a telescopic rod (41) is arranged on the cylinder (4), the bottom end of the first round cover (5) is movably inserted into the cylinder (25), a bearing 1 (51) is fixedly mounted on the middle part of the first round cover (5), the telescopic rod (41) is rotatably connected to the first round cover (5) via the bearing 1 (51), both side ends of the first round cover (5) are fixedly connected with positioning blocks (52), the two positioning blocks (52) are respectively movably sleeved on the upper ends of the two insertion rods (26), a stirring structure is arranged in the culture device housing (1), the stirring structure comprises a second round cover (6), a stirring rod (7) and a connecting block (8), a driving structure for driving the support seat (2) to move is arranged on the culture device housing (1), the driving structure comprises a motor 3 (9); A second motor (61) is fixedly mounted on the upper end of the second round cover (6), a second bearing (62) is fixedly mounted on the middle portion of the second round cover (6), and the stirring rod (7) movably passes through the second round cover (6) and is rotatably connected to the second round cover (6) via the second bearing (62); A stirring rod (71) is fixedly mounted on the stirring rod (7), the connecting block (8) is fixedly mounted on the telescopic rod (41), the end of the connecting block (8) is fixedly connected to a fixing rod (81), and the connecting block (8) is fixedly connected to the second round cover (6) via the fixing rod (81); Both side ends of the connection block (8) are fixedly connected to support rods (82), and both side ends of the second round cover (6) are fixedly connected to rectangular rods (63), the support rods (82) are fixedly connected to the corresponding rectangular rods (63), and the fixed rod (81) is located between the two support rods (82); The bottom surface of the culture device box (1) is provided with a slide groove (21), the two side ends of the support seat (2) are fixedly connected with connecting rods (22), and the upper end of the support seat (2) is fixedly installed with three round rods (29), and the three round rods (29) are distributed in a circle; The culture device box (1) is provided with a sliding door (11), and the inner wall surfaces on both sides of the culture device box (1) are provided with sliding grooves (12) in the horizontal direction, and the two connecting rods (22) are respectively slidably arranged in the sliding grooves (12), and the bottom surface of the inner wall of the culture device box (1) is fixedly connected to a sliding rod (15), and the end of the sliding rod (15) is fixedly connected to a baffle (16); Two inner wall surfaces on both sides of the culture device box (1) are provided with sliding grooves (13) in the vertical direction; the sliding grooves (13) are slidably connected to the corresponding rectangular rods (63).

2. A culture device for preparing a large intestine cell extract according to claim 1, characterized in that: The baffle (16) movably passes through the slide groove three (21), and the bottom surface of the circular plate (23) is provided with an annular groove (27).

3. A culture device for preparing a large intestine cell extract according to claim 2, characterized in that: A through opening (14) is provided on the upper surface of the culture device case (1); a circular ring (17) communicating with the through opening (14) is fixedly mounted on the upper end of the culture device case (1); a fan is arranged inside the circular ring (17); and a detachable dust screen (18) is mounted on the upper end of the circular ring (17).

4. A culture device for preparing a large intestine cell extract according to claim 1, characterized in that: The motor three (9) is fixedly mounted at the end of the culture device housing (1); the output shaft three of the motor three (9) is fixedly connected to a threaded rod (91); the threaded rod (91) is rotatably mounted in the culture device housing (1); the threaded rod (91) passes through the support seat (2) and is threadedly connected to the support seat (2).

5. A method for using a culture device for preparing a large intestine cell extract according to any one of claims 1 to 4, characterized in that: The following steps are involved: S1: Pour large intestinal cells and a certain amount of culture medium into the cylinder (25); S2: The stirring rod (7) is rotated to drive the stirring bar (71) to rotate, thereby stirring and mixing the colon cells and the culture medium in the cylinder (25); S3: After the reaction between the colon cells and the culture medium in the cylinder (25) is completed, the motor 1 (3) is started to rotate the circular plate (23). The rotation of the circular plate (23) drives the cylinder (25) and the first circular cover (5) to rotate, and the colon cells in the cylinder (25) that have completed the reaction are centrifuged.

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