A device for completely decellularizing amniotic membrane by flat-laying fixation

Abstract

The present invention discloses a device for completely decellularizing amniotic membrane by flattening and fixing in the field of experimental devices, including a box body, clamping pliers are arranged oppositely on both sides of the width of the box body, a pocket groove is arranged between the clamping pliers, the surface of the pocket groove has a through hole, a receiving group is arranged below the pocket groove, the receiving group includes a fitting bowl, a telescopic adjustment rod, a shaft connector, a driving shaft and a rotating motor connected in sequence from the top of the box body to the bottom of the box body, the pocket groove is located in the movement stroke of the fitting bowl, the end of the telescopic adjustment rod away from the fitting bowl is connected to the driving shaft through the shaft connector, and the end of the driving shaft away from the telescopic adjustment rod is connected to the rotating motor. In this technical solution, the rise and fall of the fitting bowl is used to change the amniotic membrane environment, and the amniotic membrane can be converted into an immersion environment and a drying environment. At the same time, the amniotic membrane is stretched and suspended in the air by the clamping pliers, so as to avoid the contact surface of the amniotic membrane and the container from being covered, thereby increasing the area of ​​immersion and cleaning, and reducing the time requirement for clamping the amniotic membrane for surface replacement.

Description

Technical Field

[0001] The invention belongs to the field of experimental devices, in particular to an amniotic membrane flat-laying fixed complete decellularization device. Background Art

[0002] The amnion is a translucent membrane attached to the fetus. The surface of the amnion is smooth, without blood vessels, nerves or lymph. The normal thickness of the amnion is 0.02-0.05 mm. Through an electron microscope, it can be seen that there are microvilli on the surface of the amniotic epithelial cells, which constitute an important exchange site between the amniotic fluid and the amnion. Because it contains growth factors, extremely low immunogenicity, antibacterial, anti-inflammatory, anti-fibrosis, and inhibits the formation of new blood vessels, it has been used in the medical field for more than 100 years. Its main uses include repairing ocular surface diseases, repairing tympanic membrane injuries, and covering the surface after burns.

[0003] The prior art CN1369555A discloses an amniotic membrane tissue engineering scaffold and an amniotic membrane decellularization method. This method uses amniotic membrane as a tissue engineering scaffold to provide a sufficient preparation source, but this method requires the addition of multiple reagents, and the dry and wet environments of the amniotic membrane need to be changed when extracting the amniotic membrane as a tissue engineering scaffold (for example, a wet environment of reagents is required for soaking, but a dry environment is required during scraping to avoid surface cells from aggregating and adhering in a wet environment). Currently, there is no device that can achieve the change of dry and wet environments, and multiple devices need to be combined to generate, which reduces efficiency. Summary of the invention

[0004] In order to solve the above problems, the purpose of the present invention is to provide an amniotic membrane flat-laying fixed complete decellularization device to achieve the conversion of dry and wet environments in one device, saving time for changing instruments.

[0005] In order to achieve the above-mentioned purpose, the technical solution of the present invention is as follows: A device for completely decellularizing amniotic membrane by flattening and fixing it comprises a box body, clamping clamps are arranged opposite to each other on both sides of the width of the box body, a pocket groove is arranged between the clamps, the surface of the pocket groove has a through hole, a receiving group is arranged below the pocket groove, the receiving group comprises a fitting bowl, a telescopic adjustment rod, a shaft connector, a driving shaft and a rotating motor which are connected in sequence from the top of the box body to the bottom of the box body, the telescopic adjustment rod is connected to the adjustment motor, the telescopic adjustment rod is fixedly connected to the bottom of the fitting bowl, the pocket groove is located in the movement range of the fitting bowl, the end of the telescopic adjustment rod away from the fitting bowl is connected to the driving shaft through the shaft connector, and the end of the driving shaft away from the telescopic adjustment rod is connected to the rotating motor.

[0006] The telescopic adjustment rod in the present technical solution includes but is not limited to the structure of CN205268076U.

[0007] The following beneficial effects are achieved after adopting the above scheme: In this technical scheme, the rise and fall of the fitting bowl is utilized to change the environment of the amniotic membrane, and the amniotic membrane can be converted into an immersion environment and a drying environment. At the same time, the amniotic membrane is stretched and suspended in the air by the clamping forceps, which avoids the contact surface between the amniotic membrane and the container from being covered, thereby increasing the immersion and cleaning area and reducing the time requirement for clamping the amniotic membrane for surface replacement.

[0008] Further, the pocket groove has an interlayer, a convex column is provided at the center of the pocket groove, the convex column is slidably connected to the pocket groove, and the bottom of the convex column contacts a lifting group, the lifting group is located in the interlayer, the lifting group includes a rectangular plate, a cam, a hairspring, a first tension spring, a spring rod and a base, the hairspring is connected to the base through the first tension spring, the cam is hinged to the surface of the rectangular plate, a torsion spring is provided at the hinge of the cam and the rectangular plate, and the cam is in a horizontal state in the natural state of the torsion spring;

[0009] A spring rod is connected between the base and the rectangular plate, a slideway is provided on the surface of the rectangular plate, the hairspring is slidably connected to the slideway, and when the hairspring slides along the lowest point of the slideway to the highest point of the slideway, the cam is transformed from a horizontal state to a vertical state.

[0010] Beneficial effect: This technical solution utilizes the convex column to transform the state of the amniotic membrane. During the immersion stage, the amniotic membrane is concave, and when the convex column is lifted, the amniotic membrane convexes after being subjected to force. The convex amniotic membrane facilitates the sliding of the cleaning liquid along the surface of the amniotic membrane, and the convex position of the amniotic membrane is convenient for brushing and cleaning.

[0011] Furthermore, the slide includes a first vertical slide, a second vertical slide and an inclined slide. The first vertical slide and the second vertical slide are connected by an arc-shaped transition. The inclined slide is connected between the first vertical slide and the second vertical slide. The inclined slide coincides with the contour line of the cam in the horizontal state. A vertical slide groove is opened at the vertical center line of the cam, and the slide groove coincides with the second vertical slide.

[0012] Beneficial effect: When the technical solution is implemented, the lifting plate is bendable, and the rising hairspring will slide along the first vertical slide. During the sliding process, the hairspring will convert the horizontal cam into a vertical cam. At this time, the vertical cam will push up the protrusion, thereby raising the amniotic membrane. Then the spring rod returns to its position, and the hairspring descends along the second vertical slide, thereby jamming the cam. At the same time, the first tension spring under the hairspring bends and the hairspring is offset. At this time, the restoring force of the first tension spring and the cam are not on the same horizontal line. Therefore, the restoring force of the first tension spring produces a locking effect, keeping the cam in the ejected state.

[0013] When it needs to return to its original position, you only need to bend the lifting plate again. At this time, the hairspring slides out along the second vertical slide. The cam is in a free state when it does not contact the hairspring. Therefore, the cam returns to a horizontal state under the drive of the torsion spring, so that the cam's slide groove is staggered with the second vertical slide. After the hairspring is lifted up, it will slide along the contour line of the cam driven by the first tension spring. When sliding, the hairspring slides down along the oblique slide to return to its original position.

[0014] Furthermore, a sealing ring is provided at the overlap of the boss and the pocket groove to provide waterproofing.

[0015] Furthermore, a liquid bottle is provided below the fitting bowl, a liquid inlet pipe is connected between the liquid bottle and the fitting bowl, the liquid inlet pipe and the liquid bottle are threadedly connected, an electric control valve is provided at the connection between the liquid inlet pipe and the fitting bowl, a start button is electrically connected to the electric control valve, and the start button is located at the top of the box body. It is convenient for the liquid bottle to hold liquid and new reagents can be added again.

[0016] Furthermore, the clamping forceps are slidably connected to the top of the box body to accommodate amniotic membranes of different sizes.

[0017] Furthermore, a box door is hinged on the surface of the box body, and a handle is provided on the surface of the box door. The box door is opened by the handle to replace the liquid bottle.

[0018] Furthermore, the surface of the box body is provided with an opening, the surface of the base is integrally manufactured with a shifting block, a second tension spring is provided between the shifting block and the opening, and the shifting block slides along the length direction of the opening, and the base is shifted along the shifting speed. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a schematic diagram of an embodiment of the present invention;

[0020] Figure 2 This is the structural diagram of the jacking group. DETAILED DESCRIPTION

[0021] The following is further described in detail through specific implementation methods:

[0022] The figure marks in the drawings of the specification include: box body 1, box door 2, handle 3, clamping pliers 4, pocket groove 5, through hole 6, fitting bowl 7, telescopic adjustment rod 8, shaft connector 9, driving shaft 10, rotating motor 11, adjusting motor 12, liquid storage bottle 13, liquid inlet pipe 14, electric control valve 15, start button 16, boss 17, lifting group 18, rectangular plate 181, cam 182, hairspring 183, first tension spring 184, spring rod 185, base 186, first vertical slide 19, second vertical slide 20, inclined slide 21, shift block 22, second tension spring 23, slide groove 24.

[0023] Embodiment 1

[0024] The embodiment is basically as shown in the attached Figure 1As shown: an amniotic membrane flat-laying fixed complete decellularization device includes a box body 1, a box door 2 is hinged on the surface of the box body 1, and a handle 3 is provided on the surface of the box door 2. Clamping clamps 4 are oppositely arranged on both sides of the width of the box body 1, and the clamping clamps 4 are slidably connected to the top of the box body 1. A pocket groove 5 is provided between the clamping clamps 4, and the surface of the pocket groove 5 has a through hole 6. A receiving group is provided below the pocket groove 5, and the receiving group includes a fitting bowl 7, a telescopic adjustment rod 8, a shaft connector 9, a driving shaft 10 and a rotating motor 11 connected in sequence from the top of the box body 1 to the bottom of the box body 1, the telescopic adjustment rod 8 is connected to the adjustment motor 12, the telescopic adjustment rod 8 is fixedly connected to the bottom of the fitting bowl 7, the pocket groove 5 is located in the movement stroke of the fitting bowl 7, the end of the telescopic adjustment rod 8 away from the fitting bowl 7 is connected to the driving shaft 10 through the shaft connector 9, and the end of the driving shaft 10 away from the telescopic adjustment rod 8 is connected to the rotating motor 11.

[0025] A liquid bottle 13 is provided below the bonding bowl 7, a liquid inlet pipe 14 is connected between the liquid bottle 13 and the bonding bowl 7, the liquid inlet pipe 14 and the liquid bottle 13 are threadedly connected, an electric control valve 15 is provided at the connection between the liquid inlet pipe 14 and the bonding bowl 7, the electric control valve 15 is electrically connected to a start button 16, and the start button 16 is located at the top of the box body 1.

[0026] The specific implementation process is as follows: when the present technical solution is used, the amniotic membrane is clamped by the clamping forceps 4, and then the position of the clamping forceps 4 is adjusted so that the amniotic membrane is concave in the pocket groove 5. At this time, the clamping forceps 4 serves as a supporting point, and then the pocket groove 5 is perfused with trypsin solution. At this time, trypsin slides into the fitting bowl 7 along the through hole 6. At this time, the fitting bowl 7 is gradually filled with trypsin solution, and then the adjusting motor 12 is started to move the telescopic adjusting rod 8 upward. During the upward process, the telescopic adjusting rod 8 fits the fitting bowl 7 and the pocket groove 5. At this time, the trypsin solution wraps the amniotic membrane through the through hole 6, so that the amniotic membrane is in an immersed state. When the amniotic membrane is completely immersed (such as 30 minutes), the trypsin solution can be replaced, and then the rotating motor 11 is started to cause the fitting bowl 7 to rotate. During the rotation, stirring and flushing are performed to wash away the epithelial cells of the amniotic epithelial cell layer and the superficial fibroblasts of the fibroblast layer. The washed away cells fall into the fitting bowl 7 under the drive of gravity and water flow, thereby obtaining acellular amniotic membrane.

[0027] The amniotic membrane environment can be changed by rising and falling of the fitting bowl 7, and the amniotic membrane can be converted into a soaking environment and a drying environment. At the same time, the amniotic membrane is stretched and suspended in the air by the clamping forceps 4, which avoids the contact surface between the amniotic membrane and the container from being covered, thereby increasing the soaking and cleaning area and reducing the time requirement for clamping the amniotic membrane surface.

[0028] Embodiment 2

[0029] Please refer to Figure 2The difference between the present technical solution and the above-mentioned solution is that: the pocket groove 5 has an interlayer, a boss 17 is provided at the center of the pocket groove 5, a sealing ring is provided at the overlap of the boss 17 and the pocket groove 5, the boss 17 is slidably connected with the pocket groove 5, and the bottom of the boss 17 contacts a lifting group 18, the lifting group 18 is located in the interlayer, the lifting group 18 includes a rectangular plate 181, a cam 182, a hairspring 183, a first tension spring 184, a spring rod 185 and a base 186, the hairspring 183 is connected to the base 186 through the first tension spring 184, the cam 182 is hinged to the surface of the rectangular plate 181, a torsion spring is provided at the hinge of the cam 182 and the rectangular plate 181, and the cam 182 is in a horizontal state in the natural state of the torsion spring;

[0030] A spring rod 185 is connected between the base 186 and the rectangular plate 181, a slideway is provided on the surface of the rectangular plate 181, a hairspring 183 is slidably connected to the slideway, and when the hairspring 183 slides along the lowest point of the slideway to the highest point of the slideway, the cam 182 is transformed from a horizontal state to a vertical state, the slideway includes a first vertical slideway 19, a second vertical slideway 20 and an inclined slideway 21, the first vertical slideway 19 and the second vertical slideway 20 are connected in an arc-shaped transition, the first vertical slideway 19 and the second vertical slideway 20 are connected with the inclined slideway 21, the inclined slideway 21 coincides with the contour line of the cam 182 in the horizontal state, a vertical slideway 24 is provided at the vertical center line of the cam 182, the slideway 24 coincides with the second vertical slideway 20, the surface of the box body 1 has an opening, a shift block 22 is integrally manufactured on the surface of the base 186, a second tension spring 23 is provided between the shift block 22 and the opening, and the shift block 22 slides along the length direction of the opening.

[0031] When the technical solution is implemented, the lifting plate is bent and the rising hairspring 183 will slide along the first vertical slide 19. During the sliding process, the hairspring 183 will convert the horizontal cam 182 into a vertical cam 182. At this time, the vertical cam 182 will push up the boss 17, thereby raising the amniotic membrane. Then the spring rod 185 returns to its position, and the hairspring 183 descends along the second vertical slide 20, thereby jamming the cam 182. At the same time, the first tension spring 184 under the hairspring 183 bends, and the hairspring 183 is offset. At this time, the restoring force of the first tension spring 184 and the cam 182 are not on the same horizontal line, so the restoring force of the first tension spring 184 produces a locking effect, keeping the cam 182 in the ejected state.

[0032] When it is necessary to return to the original position, it is only necessary to bend the lifting plate again. At this time, the hairspring 183 slides out along the second vertical slide. The cam 182 is in a free state when it does not contact the hairspring 183. Therefore, the cam 182 returns to the horizontal state under the drive of the torsion spring, so that the slide groove 24 of the cam 182 is staggered with the second vertical slide 20. After the hairspring 183 is lifted up, it will slide along the contour line of the cam 182 driven by the first tension spring 184. When sliding, the hairspring 183 slides down along the oblique slide 21 to return to the original position.

[0033] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0034] The above is only an embodiment of the present invention. The common sense such as the known specific structure and characteristics in the scheme is not described in detail here. The ordinary technicians in the relevant field know all the common technical knowledge in the technical field of the invention before the application date or priority date, can obtain all the existing technologies in the field, and have the ability to apply the conventional experimental means before that date. The ordinary technicians in the relevant field can improve and implement this scheme in combination with their own abilities under the enlightenment given by this application. Some typical known structures or known methods should not become obstacles for ordinary technicians in the relevant field to implement this application. It should be pointed out that for those skilled in the art, without departing from the structure of the present invention, several deformations and improvements can be made, which should also be regarded as the protection scope of the present invention, which will not affect the effect of the implementation of the present invention and the practicality of the patent. The protection scope required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A device for completely decellularizing amniotic membrane by flattening and fixing, characterized in that: The invention comprises a box body, clamping pliers are arranged opposite to each other on both sides of the width of the box body, a pocket groove is arranged between the clamping pliers, a through hole is arranged on the surface of the pocket groove, a receiving group is arranged below the pocket groove, the receiving group comprises a fitting bowl, a telescopic adjustment rod, a shaft connector, a driving shaft and a rotating motor connected in sequence from the top of the box body to the bottom of the box body, the telescopic adjustment rod is connected to the adjusting motor, the telescopic adjustment rod is fixedly connected to the lower part of the fitting bowl, the pocket groove is located in the movement stroke of the fitting bowl, the end of the telescopic adjustment rod away from the fitting bowl is connected to the driving shaft through the shaft connector, and the end of the driving shaft away from the telescopic adjustment rod is connected to the rotating motor; A liquid holding bottle is provided below the laminating bowl, a liquid inlet pipe is connected between the liquid holding bottle and the laminating bowl, the liquid inlet pipe and the liquid holding bottle are threadedly connected, an electric control valve is provided at the connection between the liquid inlet pipe and the laminating bowl, the electric control valve is electrically connected to a start button, and the start button is located at the top of the box body; The clamping jaws are slidably connected to the top of the box body.

2. The device for completely decellularizing amniotic membrane by flat-laying fixation according to claim 1, characterized in that: The pocket groove has an interlayer, a convex column is provided at the center of the pocket groove, the convex column is slidably connected to the pocket groove, and the bottom of the convex column contacts a lifting group, the lifting group is located in the interlayer, the lifting group includes a rectangular plate, a cam, a hairspring, a first tension spring, a spring rod and a base, the hairspring is connected to the base through the first tension spring, the cam is hinged to the surface of the rectangular plate, a torsion spring is provided at the hinge of the cam and the rectangular plate, and the cam is in a horizontal state in the natural state of the torsion spring; A spring rod is connected between the base and the rectangular plate, a slideway is provided on the surface of the rectangular plate, the hairspring is slidably connected to the slideway, and when the hairspring slides along the lowest point of the slideway to the highest point of the slideway, the cam is transformed from a horizontal state to a vertical state.

3. The device for completely decellularizing amniotic membrane by flat-laying fixation according to claim 2, characterized in that: The slide includes a first vertical slide, a second vertical slide and an inclined slide. The first vertical slide and the second vertical slide are connected in an arc transition. The inclined slide is connected between the first vertical slide and the second vertical slide. The inclined slide coincides with the contour line of the cam in a horizontal state. A vertical slide groove is opened at the vertical center line of the cam, and the slide groove coincides with the second vertical slide.

4. The device for completely decellularizing amniotic membrane by flat-laying fixation according to claim 2, characterized in that: The overlapping part of the boss and the pocket groove is provided with a sealing ring.

5. The device for completely decellularizing amniotic membrane by flat-laying fixation according to claim 1, characterized in that: A door is hinged on the surface of the box body, and a handle is provided on the surface of the door.

6. The device for completely decellularizing amniotic membrane by flat-laying fixation according to claim 2, characterized in that: The surface of the box body is provided with an opening, the surface of the base is integrally manufactured with a shifting block, a second tension spring is provided between the shifting block and the opening, and the shifting block slides along the length direction of the opening.

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