Microbial flora storage device and method of storing same

Abstract

The application discloses a kind of microbial flora storage device and its storage method, including jar body, the top of jar body is equipped with seal door, the inner wall of jar body is rotatably connected with outer shaft sleeve, outer shaft sleeve is fixedly installed with transmission gear on the circumferential surface, the inner wall of outer shaft sleeve is rotatably connected with inner spiral pipe, the inner wall of inner spiral pipe is rotatably connected with cloth cold axle cylinder, the circumferential surface of jar body is respectively fixedly installed with servo motor and refrigeration mechanism, the output shaft end of servo motor is respectively connected with outer shaft sleeve and inner spiral pipe transmission, the port of refrigeration mechanism is fixedly communicated with cloth cold axle cylinder, the circumferential surface of cloth cold axle cylinder is equipped with several groups of air supply holes that are distributed in circular array, and the circumferential surface of inner spiral pipe is fixedly provided with main rotating seat.The application is placed module, refrigeration mechanism and other structures are set, so that the device can efficiently complete the storage culture operation of microbial flora, and the device changes the static storage structure of traditional storage device to dynamic storage structure when storing microbial flora.

Description

Technical Field

[0001] This invention relates to the field of microbial community storage devices, and more specifically, to a microbial community storage device and its storage method. Background Technology

[0002] The human gastrointestinal tract is home to a wide variety of microorganisms, known as the gut microbiota. These microorganisms are combined in a certain proportion, and each microorganism restrains and depends on the others, forming an ecological balance in terms of both quality and quantity.

[0003] The gut microbiota responds to the nutrients consumed by the host through various hormones and to the host's state. They also produce compounds that signal systems within the body, including neurotransmitters (such as γ-aminobutyric acid), amino acids (such as tyrosine and tryptophan; tryptophan can be converted into mood-regulating molecules, dopamine, and serotonin), and many other substances. These nutrients play a vital role in human health, and their deficiency can lead to various diseases. During the cultivation of gut microbiota, low-temperature storage is necessary. Existing storage devices are prone to uneven cooling of the microbiota during storage, and the distribution of the microbiota in the culture medium is also inconsistent. Therefore, this invention provides a microbial microbiota storage device and method to address the problems mentioned in the background section. Summary of the Invention

[0004] (a) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, the present invention aims to provide a microbial community storage device and storage method, thereby solving the problems that existing storage devices are prone to uneven cooling of the microbial community during storage operations, and that the distribution of the microbial community in the culture medium is uneven during storage.

[0006] (II) Technical Solution

[0007] To achieve the above objectives, the present invention provides the following technical solution: a microbial community storage device comprising a tank, a sealing door installed on the top of the tank, an outer bushing rotatably connected to the inner wall of the tank, a transmission gear fixedly installed on the circumferential side of the outer bushing, an inner spiral tube rotatably connected to the inner wall of the outer bushing, a cooling shaft rotatably connected to the inner wall of the inner spiral tube, a servo motor and a refrigeration mechanism fixedly installed on the circumferential side of the tank, and the output shaft end of the servo motor being connected to the outer bushing and the inner spiral tube respectively. The cooling mechanism is connected to a cooling shaft. The cooling shaft has several sets of air outlets arranged in a circular array on its circumferential side. A main rotating seat is fixedly mounted on the circumferential side of the inner rotating tube. The main rotating seat is rotatably connected to the tank body. A set of placement modules arranged in a circular array is fixedly installed on the inner wall of the main rotating seat. The inner walls of each placement module are connected to a transmission gear. A vibration mechanism that cooperates with the placement modules is fixedly installed at the bottom of the tank body. An auxiliary module is fixedly installed on the upper part of the tank body.

[0008] As a preferred embodiment, the placement module includes a spin seat and a lower gear ring mounted on the bottom surface of the main spin seat. The circumferential side of the spin seat is rotatably connected to the main spin seat. An upper gear ring, which is connected to a transmission gear, is fixedly mounted on the top surface of the spin seat. A set of placement cylinders arranged in a circular array are rotatably connected to the inner wall of the spin seat. A driven gear is fixedly mounted on the circumferential side of each placement cylinder. The circumferential side of the driven gear is connected to the lower gear ring. A culture tube with an open top is clamped into the inner wall of the placement cylinder. An air injection clamp is rotatably connected to the bottom axis of the spin seat. A set of clamps arranged in a circular array are fixedly connected to the circumferential side of the air injection clamp. The circumferential side of each clamp cooperates with the culture tube. The bottom end of the air injection clamp is rotatably connected to the excitation mechanism.

[0009] As a preferred embodiment, the vibration mechanism includes an air pump, an air guide swirl seat, an electromagnetic ring, and a vibration cylinder. The top surface of the air pump is fixedly connected to the tank body, the peripheral side of the air guide swirl seat is rotatably connected to the tank body, one end of the air pump outlet is rotatably connected to the air guide swirl seat, the top surface of the air guide swirl seat is rotatably connected to the air injection clamp, the bottom end of the cooling shaft cylinder is rotatably connected to the air guide swirl seat, a support ring is fixedly installed on the inner wall of the vibration cylinder at the position corresponding to the bottom of each placement module, the inner wall of the vibration cylinder is slidably connected to the air guide swirl seat, a set of anti-compression springs arranged in a circular array are installed between the opposing surfaces of the vibration cylinder and the air guide swirl seat, and a permanent magnet ring that cooperates with the electromagnetic ring is fixedly installed on the bottom surface of the vibration cylinder, the electromagnetic ring is located below the permanent magnet ring and the bottom surface of the electromagnetic ring is fixedly connected to the tank body.

[0010] As a preferred embodiment, a pressure sensor is fixedly installed at the connection between the air pump and the air guide swirl seat. The air guide swirl seat has a hollow structure. A sealing ring that fits against the tank body is fixedly installed on the peripheral side of the excitation cylinder. A guide hole that slides with the air guide swirl seat is fixedly opened on the inner wall of the excitation cylinder. The cross-section of the guide hole is a regular polygon. A guide sleeve that matches the shape of the guide hole is fixedly installed at the bottom of the air guide swirl seat.

[0011] As a preferred embodiment, the magnetism of the electromagnetic ring is the same as that of the permanent magnet ring.

[0012] As a preferred embodiment, the refrigeration mechanism includes a refrigerator fixedly connected to the tank body, a refrigeration annular cavity opened inside the tank body, an insulation layer disposed on the outside of the tank body, and a refrigeration coil installed on the inner wall of the refrigeration annular cavity. One end of the return air port of the refrigerator is connected to the air outlet of the refrigeration coil, one end of the air outlet of the refrigerator is connected to a main air supply pipe, one end of the air outlet of the main air supply pipe is connected to the air inlet of the refrigeration coil, the top end of the main air supply pipe is fixedly connected to a branch air supply pipe, and the peripheral side of the branch air supply pipe is fixedly connected to the cooling shaft cylinder.

[0013] As a preferred embodiment, the auxiliary module includes an electrical control base fixedly connected to the tank body, a temperature probe and an oxygen concentration sensor installed on the top of the tank body. The electrical control base has a built-in microcontroller. The monitoring ends of the temperature probe and the oxygen concentration sensor extend into the interior of the tank body. The data ends of the temperature probe and the oxygen concentration sensor are electrically connected to the microcontroller. An oxygen inlet pipe and an exhaust pipe are fixedly installed on the top of the tank body. Both the oxygen inlet pipe and the exhaust pipe have air valves installed inside.

[0014] As a preferred embodiment, both the outer bushing and the inner spiral tube are hollow tubular structures with openings at both ends. Driven bevel gears are fixedly installed on the circumferential surfaces of both the outer bushing and the inner spiral tube. Two active bevel gears are installed on the output shaft end of the servo motor. The circumferential surfaces of the two active bevel gears mesh with the two driven bevel gears respectively. The two driven bevel gears are symmetrically arranged about the horizontal plane where the axis of the servo motor is located.

[0015] As a preferred embodiment, the cooling shaft cylinder is a hollow cylindrical structure with an open top and a closed bottom, and the axis of the air supply hole is perpendicular to the axis of the cooling shaft cylinder.

[0016] The present invention also provides a method for storing microbial communities, comprising the following steps:

[0017] SS001, Pre-placement: Before operation, open the sealing door and place the bacteria to be stored into each culture tube. After the bacteria are placed, place the culture tubes into the placement cylinders in each placement module in sequence. After placement, close the sealing door. After the sealing door is closed, the air pump fills each clamp with air until the monitoring value of the air pressure sensor reaches the set threshold. After the air pump finishes air intake, the oxygen inlet pipe is connected to the external oxygen supply equipment. The oxygen inlet pipe, in conjunction with the oxygen concentration sensor, keeps the oxygen concentration inside the tank constant.

[0018] SS002. Storage Operation: During storage, the refrigeration unit, in conjunction with a temperature probe, maintains the internal temperature of the tank at a set low. During storage, the servo motor operates periodically. Within the servo motor's cycle, the inner rotating tube drives the main rotating seat to revolve. During this revolve, the outer bushing drives the upper gear ring to rotate via a transmission gear. The rotation of the upper gear ring then causes the main rotating seat to rotate at a set speed simultaneously with its revolve. During this rotation, due to the meshing connection between the driven gear and the lower gear ring, the placement cylinder also rotates synchronously with the main rotating seat. Through this rotation, the storage tank... Each culture tube is cooled evenly, and the electromagnetic ring is periodically energized during the working cycle of the servo motor. During the energizing cycle of the electromagnetic ring, a repulsive force is generated between the electromagnetic ring and the permanent magnet ring. After the repulsive force is generated, the excitation cylinder moves upward. By controlling the energizing cycle of the electromagnetic ring, the up-and-down vibration frequency of the excitation cylinder can be effectively controlled. By controlling the current supplied to the electromagnetic ring, the up-and-down displacement stroke of the excitation cylinder can be effectively controlled. After the excitation cylinder vibrates, it then acts on the culture tube through the support ring. After the support ring acts on the culture tube, it causes the culture tube to vibrate up and down. When it is necessary to remove the culture tube, the sealing door is opened, and the removal of the stored culture tube can be completed.

[0019] (III) Beneficial Effects

[0020] Compared with the prior art, the present invention provides a microbial community storage device and storage method, which has the following beneficial effects.

[0021] 1. This invention, through the arrangement of placement modules, refrigeration mechanisms, and other structures, enables the device to efficiently complete the storage and cultivation of microbial communities. Furthermore, during the storage of microbial communities, this device transforms the traditional static storage structure into a dynamic storage structure. During operation, the servo motor operates periodically. Within the working cycle of the servo motor, the inner rotating tube drives the main rotating seat to revolve. During the revolve motion of the main rotating seat, the outer bushing drives the upper gear ring to rotate through the transmission gear. After the upper gear ring rotates, the main rotating seat then rotates at a set speed while revolving. When the main rotating seat rotates, due to the meshing connection between the driven gear and the lower gear ring, the placement cylinder also rotates synchronously when the main rotating seat rotates. Through the occurrence of the above rotation, each culture tube is cooled evenly.

[0022] 2. This invention adds an oscillation mechanism for the bacterial community to the traditional storage device by setting up an electromagnetic ring and a permanent magnet ring. During operation, the electromagnetic ring is periodically energized within the working cycle of the servo motor. During the energizing cycle of the electromagnetic ring, a repulsive force is generated between the electromagnetic ring and the permanent magnet ring. After the repulsive force is generated, it drives the excitation cylinder to move upward. By controlling the energizing cycle of the electromagnetic ring, the up-and-down vibration frequency of the excitation cylinder can be effectively controlled. By controlling the current supplied to the electromagnetic ring, the up-and-down displacement stroke of the excitation cylinder can be effectively controlled. After the excitation cylinder vibrates, it then acts on the culture tube through the support ring. After the support ring acts on the culture tube, it causes the culture tube to vibrate up and down. Through the occurrence of up-and-down vibration of the culture tube, the bacterial community is uniformly distributed in the culture tube. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the structure of a microbial community storage device according to the present invention;

[0024] Figure 2 For the present invention Figure 1 A schematic diagram of the cross-sectional structure;

[0025] Figure 3 For the present invention Figure 2 A magnified schematic diagram of the partial structure at point A in the middle;

[0026] Figure 4 For the present invention Figure 2 A magnified view of the structure at point B in the middle;

[0027] Figure 5 For the present invention Figure 2 A magnified schematic diagram of the local structure at point C;

[0028] Figure 6 This is a schematic diagram of the structure of the excitation cylinder and the culture tube of the present invention;

[0029] Figure 7 For the present invention Figure 6 A magnified schematic diagram of the local structure at point D;

[0030] Figure 8 This is a schematic diagram of the structure when the placement module of the present invention is installed on the main rotating seat;

[0031] Figure 9 For the present invention Figure 8 A magnified schematic diagram of the local structure at point E;

[0032] Figure 10 This is a schematic diagram of the structure of the present invention, which houses the rotating cylinder and the driven gear;

[0033] Figure 11 This is a schematic diagram of the main rotating seat and air supply hole of the present invention;

[0034] Figure 12 This is a schematic diagram of the structure of the excitation cylinder of the present invention.

[0035] In the diagram: 1. Tank body; 2. Sealing door; 3. Outer bushing; 4. Transmission gear; 5. Inner swivel tube; 6. Servo motor; 7. Air supply hole; 8. Main swivel seat; 9. Placement module; 10. Spin seat; 11. Lower gear ring; 12. Upper gear ring; 13. Placement swivel cylinder; 14. Driven gear; 15. Culture tube; 16. Gas injection clamp; 17. Clamping bag; 18. Air pump; 19. Air guide swivel seat; 20. Electromagnetic ring; 21. Vibration cylinder; 22. Support ring; 23. Compression spring; 24. Permanent magnet ring; 25. Air pressure sensor; 26. Gas supply branch pipe; 27. Refrigerator; 28. Refrigeration ring cavity; 29. ​​Insulation layer; 30. Refrigeration coil; 31. Main gas supply pipe; 32. Electrical control base; 33. Temperature probe; 34. Oxygen concentration sensor; 35. Oxygen inlet pipe; 36. Exhaust pipe; 37. Cooling swivel cylinder. Detailed Implementation

[0036] The present invention will be further described and illustrated below with reference to specific embodiments and the accompanying drawings:

[0037] Please see Figure 1-12 The present invention: a microbial community storage device adopts the following technical solution: including a tank body 1, a sealing door 2 installed on the top of the tank body 1, the sealing door 2 being hinged to the tank body 1, a fastener that cooperates with the tank body 1 being fixedly provided on the surface of the sealing door 2, a lock that cooperates with the fastener being fixedly provided on the surface of the tank body 1, and a sealing ring that cooperates with the tank body 1 being lined on the inner wall of the sealing plate.

[0038] An outer bushing 3 is rotatably connected to the inner wall of the tank body 1. A transmission gear 4 is fixedly installed on the circumferential side of the outer bushing 3. An inner spiral tube 5 is rotatably connected to the inner wall of the outer bushing 3. A cooling shaft cylinder 37 is rotatably connected to the inner wall of the inner spiral tube 5. The port of the refrigeration mechanism is fixedly connected to the cooling shaft cylinder 37.

[0039] Both the outer bushing 3 and the inner spiral tube 5 are hollow tubular structures with open ends, and the cooling shaft cylinder 37 is a hollow cylindrical structure with an open top and a closed bottom.

[0040] A servo motor 6 and a refrigeration mechanism are fixedly installed on the periphery of the tank body 1, and the output shaft of the servo motor 6 is connected to the outer bushing 3 and the inner spiral tube 5 respectively.

[0041] Both the outer bushing 3 and the inner spiral tube 5 are fixedly mounted with driven bevel gears. The output shaft end of the servo motor 6 is equipped with two driving bevel gears. The peripheral surfaces of the two driving bevel gears mesh with the two driven bevel gears respectively. The two driven bevel gears are symmetrically arranged about the horizontal plane where the axis of the servo motor 6 is located.

[0042] By setting the upper arrangement of the two driven bevel gears, the rotation directions of the outer bushing 3 and the inner spiral tube 5 are reversed;

[0043] The circumferential side of the cooling shaft cylinder 37 is provided with several sets of air supply holes 7 arranged in a circular array. The axis of the air supply holes 7 is perpendicular to the axis of the cooling shaft cylinder 37. The axis of the cooling shaft cylinder 37 is on the same straight line as the axis of the tank body 1. The diameter of the air supply holes 7 can be customized according to actual needs.

[0044] The inner swivel tube 5 is fixedly mounted with a main swivel seat 8. The circumferential side of the main swivel seat 8 is rotatably connected to the tank body 1. A set of placement modules 9 arranged in a circular array are fixedly installed on the inner wall of the main swivel seat 8. The inner walls of the set of placement modules 9 are all connected to the transmission gear 4. The bottom of the tank body 1 is fixedly mounted with a vibration mechanism that cooperates with the placement modules 9. The upper part of the tank body 1 is fixedly mounted with an auxiliary module.

[0045] The placement module 9 includes a spin seat 10 and a lower gear ring 11 mounted on the bottom surface of the main spin seat 8. The lower gear ring 11 and the main spin seat 8 are coaxially arranged.

[0046] The circumferential side of the spin seat 10 is rotatably connected to the main spin seat 8. The top surface of the spin seat 10 is fixedly installed with an upper gear ring 12 that is connected to the transmission gear 4. The inner wall of the spin seat 10 is rotatably connected with a set of placement cylinders 13 arranged in a circular array. The placement cylinders 13 are hollow cylindrical structures with openings at both ends.

[0047] Each placement cylinder 13 has a driven gear 14 fixedly installed on its circumferential side. The circumferential side of the driven gear 14 is connected to the lower gear ring 11 for transmission. The inner wall of the placement cylinder 13 is fitted with a culture tube 15 with an open top. The culture tube 15 is made of glass.

[0048] An air injection clamp tube 16 is rotatably connected to the bottom axis of the spin seat 10. A set of clamps 17 arranged in a circular array are fixedly connected to the circumferential side of the air injection clamp tube 16. The circumferential side of each clamp 17 is matched with the culture tube 15. The bottom end of the air injection clamp tube 16 is rotatably connected to the excitation mechanism.

[0049] The excitation mechanism includes an air pump 18, an air guide swirl seat 19, an electromagnetic ring 20, and an excitation cylinder 21. The air guide swirl seat 19 has a hollow structure, and a sealing ring that fits into the tank body 1 is fixedly installed on the peripheral side of the excitation cylinder 21.

[0050] The top surface of the air pump 18 is fixedly connected to the tank body 1, the peripheral side of the air guide swivel seat 19 is rotatably connected to the tank body 1, one end of the air outlet of the air pump 18 is rotatably connected to the air guide swivel seat 19, and a pressure sensor 25 is fixedly installed at the connection between the air pump 18 and the air guide swivel seat 19. The function of the pressure sensor 25 is to assist in monitoring the clamping strength of the clamping bag 17.

[0051] When in use, the air pressure sensor 25 feeds back the monitored data to the microcontroller inside the electrical control base 32 in real time. The microcontroller controls the working status of the air pump 18 based on the data feedback from the air pressure sensor 25.

[0052] The model number of the barometric pressure sensor 25 is QMP6988;

[0053] Air pump 18 is a dual-purpose pump for both suction and extraction;

[0054] The top surface of the air guide swirl seat 19 is rotatably connected to the air injection clamp tube 16, the bottom end of the cooling shaft cylinder 37 is rotatably connected to the air guide swirl seat 19, and the inner wall of the excitation cylinder 21 is fixedly installed with a support ring 22 corresponding to the bottom position of each placement module 9. The inner wall of the excitation cylinder 21 is slidably connected to the air guide swirl seat 19. The inner wall of the excitation cylinder 21 is fixedly provided with a guide hole that slides with the air guide swirl seat 19. The cross-section of the guide hole is a regular polygon. The bottom of the air guide swirl seat 19 is fixedly provided with a guide sleeve that matches the shape of the guide hole.

[0055] By setting the regular polygonal structure of the guide hole, on the one hand, it is ensured that the excitation cylinder 21 can slide up and down along the air guide swirl seat 19, and on the other hand, it is ensured that the excitation cylinder 21 can rotate synchronously with the air guide swirl seat 19.

[0056] A set of anti-compression springs 23 arranged in a circular array are installed between the opposing surfaces of the excitation cylinder 21 and the air guide swirl seat 19. A permanent magnet ring 24 that cooperates with the electromagnetic ring 20 is fixedly installed on the bottom surface of the excitation cylinder 21. The electromagnetic ring 20 is located below the permanent magnet ring 24 and the bottom surface of the electromagnetic ring 20 is fixedly connected to the tank body 1. The magnetism of the electromagnetic ring 20 is the same as that of the permanent magnet ring 24.

[0057] The permanent magnet ring 24 is essentially a permanent magnet, and the electromagnetic ring 20 is essentially an electromagnet. The electromagnet generates magnetism when energized and loses magnetism when de-energized.

[0058] The refrigeration mechanism includes a refrigerator 27 fixedly connected to the tank body 1, a refrigeration annular cavity 28 opened inside the tank body 1, an insulation layer 29 disposed on the outside of the tank body 1, and a refrigeration coil 30 installed on the inner wall of the refrigeration annular cavity 28.

[0059] The refrigerator 27 is a commonly used device in the prior art, and will not be described in detail here. The function of the refrigerator 27 is to supply cold air.

[0060] The model number of the cooler 27 is XH-X266;

[0061] One end of the return air port of the refrigeration unit 27 is connected to the air outlet of the refrigeration coil 30. One end of the air outlet of the refrigeration unit 27 is connected to the main air supply pipe 31. One end of the air outlet of the main air supply pipe 31 is connected to the air inlet of the refrigeration coil 30. The top end of the main air supply pipe 31 is fixedly connected to the air supply branch pipe 26. The circumferential side of the air supply branch pipe 26 is fixedly connected to the cooling shaft cylinder 37.

[0062] The auxiliary module includes an electrical control base 32 fixedly connected to the tank body 1, a temperature probe 33 and an oxygen concentration sensor 34 installed on the top of the tank body 1. The electrical control base 32 has a built-in microcontroller. The monitoring ends of the temperature probe 33 and the oxygen concentration sensor 34 extend into the interior of the tank body 1. The data ends of the temperature probe 33 and the oxygen concentration sensor 34 are electrically connected to the microcontroller. An oxygen inlet pipe 35 and an exhaust pipe 36 are fixedly installed on the top of the tank body 1. Both the oxygen inlet pipe 35 and the exhaust pipe 36 have air valves installed inside.

[0063] The temperature probe 33 is model DS18B20;

[0064] The oxygen concentration sensor 34 is model OOM102-1;

[0065] The microcontroller model is FX1N-60MR-001;

[0066] A method for storing microbial flora, comprising the following steps:

[0067] SS001, Pre-placement: Before operation, open the sealing door 2 and place the bacteria to be stored into each culture tube 15. After the bacteria are placed, place the culture tubes 15 into the placement cylinders 13 in each placement module 9 in sequence. After placement, close the sealing door 2. After the sealing door 2 is closed, the air pump 18 inflates each clamp 17 until the monitoring value of the air pressure sensor 25 reaches the set threshold. After the air pump 18 finishes inhaling air, the oxygen inlet pipe 35 is connected to the external oxygen supply equipment. The oxygen inlet pipe 35, in cooperation with the oxygen concentration sensor 34, keeps the oxygen concentration inside the tank 1 constant.

[0068] SS002, Storage Operation: During storage, the cooler 27, in conjunction with the temperature probe 33, maintains the interior of the tank 1 at a set low temperature. During storage, the servo motor 6 operates periodically. Within the working cycle of the servo motor 6, the inner rotating tube 5 drives the main rotating seat 8 to revolve. During the revolve of the main rotating seat 8, the outer bushing 3 drives the upper gear ring 12 to rotate via the transmission gear 4. After the upper gear ring 12 rotates, the main rotating seat 8 rotates at a set speed while revolving. During the rotation of the main rotating seat 8, due to the meshing connection between the driven gear 14 and the lower gear ring 11, the placement cylinder 13 also rotates synchronously with the rotation of the main rotating seat 8. Through this rotation, each culture tube 15... The electromagnetic ring 20 is periodically energized during the working cycle of the servo motor 6, and a repulsive force is generated between the electromagnetic ring 20 and the permanent magnet ring 24 during the energizing cycle of the electromagnetic ring 20. After the repulsive force is generated, the excitation cylinder 21 is driven to move upward. By controlling the energizing cycle of the electromagnetic ring 20, the up-and-down vibration frequency of the excitation cylinder 21 can be effectively controlled. By controlling the current supplied to the electromagnetic ring 20, the up-and-down displacement stroke of the excitation cylinder 21 can be effectively controlled. After the excitation cylinder 21 vibrates, it then acts on the culture tube 15 through the support ring 22. After the support ring 22 acts on the culture tube 15, the culture tube 15 is then excited up and down. When it is necessary to take out the culture tube 15, the sealing door 2 is opened, and the removal operation of the stored culture tube 15 can be completed.

[0069] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A microbial flora storage device comprising a tank body (1), a top of the tank body (1) is provided with a sealing door (2), characterized in that: The inner wall of the tank body (1) is rotationally connected with an outer shaft sleeve (3), the peripheral surface of the outer shaft sleeve (3) is fixedly installed with a transmission gear (4), the inner wall of the outer shaft sleeve (3) is rotationally connected with an inner spiral pipe (5), the inner wall of the inner spiral pipe (5) is rotationally connected with a cold distribution shaft cylinder (37), the peripheral surface of the tank body (1) is respectively fixedly installed with a servo motor (6) and a refrigeration mechanism, the output shaft ends of the servo motor (6) are respectively in transmission connection with the outer shaft sleeve (3) and the inner spiral pipe (5), the port of the refrigeration mechanism is in fixed communication with the cold distribution shaft cylinder (37), the peripheral surface of the cold distribution shaft cylinder (37) is provided with a plurality of groups of air supply holes (7) distributed in a circumferential array, the peripheral surface of the main rotating seat (8) is rotationally connected with the tank body (1), the inner wall of the main rotating seat (8) is fixedly installed with a plurality of groups of placement modules (9) distributed in a circumferential array, the inner walls of the groups of placement modules (9) are all in transmission connection with the transmission gear (4), the bottom of the tank body (1) is fixedly installed with a vibration exciting mechanism matched with the placement modules (9), and the upper portion of the tank body (1) is fixedly installed with an auxiliary module group. The placement modules (9) respectively include a self-rotating seat (10) and a lower gear ring (11) installed at the bottom surface of the main rotating seat (8), the peripheral surface of the self-rotating seat (10) is rotationally connected with the main rotating seat (8), the top surface of the self-rotating seat (10) is fixedly installed with an upper gear ring (12) in transmission connection with the transmission gear (4), the inner wall of the self-rotating seat (10) is rotationally connected with a plurality of groups of placement rotating cylinders (13) distributed in a circumferential array, the peripheral surface of each placement rotating cylinder (13) is fixedly installed with a driven gear (14), the peripheral surface of the driven gear (14) is in transmission connection with the lower gear ring (11), the inner wall of the placement rotating cylinder (13) is clamped with a bacteria cultivation pipe (15) with an open top end, the bottom surface axial position of the self-rotating seat (10) is rotationally connected with a gas injection clamp pipe (16), the peripheral surface of the gas injection clamp pipe (16) is fixedly communicated with a plurality of groups of clamp sacs (17) distributed in a circumferential array, the peripheral surface of each clamp sac (17) is matched with the bacteria cultivation pipe (15), and the bottom end of the gas injection clamp pipe (16) is in rotational communication with the vibration exciting mechanism. The exciting mechanism respectively comprises a gas pump (18), a gas guide rotary seat (19), an electromagnetic ring (20) and an exciting cylinder (21), the top surface of the gas pump (18) is fixedly connected with the tank body (1), the peripheral surface of the gas guide rotary seat (19) is rotationally connected with the tank body (1), one end of the gas outlet of the gas pump (18) is rotationally communicated with the gas guide rotary seat (19), the top surface of the gas guide rotary seat (19) is rotationally communicated with the gas injection clamp pipe (16), the bottom end of the cold distribution shaft cylinder (37) is rotationally connected with the gas guide rotary seat (19), the inner wall of the exciting cylinder (21) is fixedly provided with a plurality of supporting rings (22) corresponding to the positions of the bottom of each placing module (9), the inner wall of the exciting cylinder (21) is slidingly connected with the gas guide rotary seat (19), a plurality of compression-resistant springs (23) in a circumferential array are arranged between the opposite surfaces of the exciting cylinder (21) and the gas guide rotary seat (19), the bottom surface of the exciting cylinder (21) is fixedly provided with a permanent magnet ring (24) matched with the electromagnetic ring (20), the electromagnetic ring (20) is arranged below the permanent magnet ring (24), and the bottom surface of the electromagnetic ring (20) is fixedly connected with the tank body (1); The magnetism of the electromagnetic ring (20) is the same as that of the permanent magnet ring (24); The outer shaft sleeve (3) and the inner rotary pipe (5) are both hollow tubular structures with open ends, the peripheral surfaces of the outer shaft sleeve (3) and the inner rotary pipe (5) are fixedly provided with driven bevel gears, the output shaft end of the servo motor (6) is provided with two driving bevel gears, the peripheral surfaces of the two driving bevel gears are respectively engaged with the two driven bevel gears, and the two driven bevel gears are symmetrically arranged with the horizontal plane of the axis of the servo motor (6) as the axis.

2. The microbial consortia storage device of claim 1, wherein: A gas pressure sensor (25) is fixedly arranged at the communication position of the gas pump (18) and the gas guide rotary seat (19), the gas guide rotary seat (19) is a hollow structure, the peripheral surface of the exciting cylinder (21) is fixedly provided with a sealing ring abutting against the tank body (1), the inner wall of the exciting cylinder (21) is fixedly provided with a guide hole slidingly matched with the gas guide rotary seat (19), the cross section of the guide hole is a regular polygon, and the bottom of the gas guide rotary seat (19) is fixedly provided with a guide sleeve matched with the shape of the guide hole.

3. The microbial consortium storage device of claim 1, wherein: The refrigeration mechanism comprises a refrigeration device (27) fixedly connected with the tank body (1), a refrigeration ring cavity (28) formed in the tank body (1), a heat preservation layer (29) arranged on the outer side of the tank body (1) and a refrigeration coil pipe (30) fixedly arranged on the inner wall of the refrigeration ring cavity (28), one end of the gas return port of the refrigeration device (27) is communicated with the gas outlet of the refrigeration coil pipe (30), one end of the gas outlet of the refrigeration device (27) is communicated with a gas supply main pipe (31), one end of the gas outlet of the gas supply main pipe (31) is communicated with the gas inlet of the refrigeration coil pipe (30), the top end of the gas supply main pipe (31) is fixedly communicated with a gas supply branch pipe (26), and the peripheral surface of the gas supply branch pipe (26) is fixedly communicated with the cold distribution shaft cylinder (37).

4. The microbial consortium storage device of claim 1, wherein: The auxiliary module respectively includes an electric control seat (32) fixedly connected with the tank body (1), a temperature probe (33) and an oxygen concentration sensor (34) installed on the top of the tank body (1), a single-chip microcomputer is built in the electric control seat (32), the monitoring ends of the temperature probe (33) and the oxygen concentration sensor (34) extend to the inside of the tank body (1), the data ends of the temperature probe (33) and the oxygen concentration sensor (34) are electrically connected with the single-chip microcomputer, the oxygen inlet pipe (35) and the exhaust pipe (36) are fixedly installed on the top of the tank body (1), and air valves are installed in the oxygen inlet pipe (35) and the exhaust pipe (36).

5. The microbial consortium storage device of claim 1, wherein: The cloth cooling shaft cylinder (37) is a hollow cylinder structure with an open top and a closed bottom, and the axis of the air supply hole (7) is perpendicular to the axis of the cloth cooling shaft cylinder (37).

6. A method for storing a microbial flora using a microbial flora storage device according to any one of claims 1 to 5, characterized by: The method comprises the following steps: SS001, pre-placing, before work, opening the sealing door (2), placing the bacteria group to be stored in each bacteria culture tube (15), placing the bacteria group, then placing the bacteria culture tube (15) in the placing rotary cylinder (13) in each placing module (9) in sequence, after placing, closing the sealing door (2), after closing the sealing door (2), the air pump (18) inflates each clamping capsule (17) until the monitoring value of the air pressure sensor (25) reaches the set threshold value, after the air pump (18) finishes inflating, the oxygen inlet pipe (35) is communicated with the external oxygen supply equipment, and the oxygen inlet pipe (35) keeps the oxygen concentration in the tank body (1) constant through cooperation with the oxygen concentration sensor (34); SS002、storage operation, when the storage operation, the refrigerator (27) by cooperating with the temperature probe (33), the internal tank (1) to maintain a set temperature, and storage operation, the servo motor (6) periodically work, in the work cycle of the servo motor (6), the inner tube (5) drive main rotating seat (8) revolution, the main rotating seat (8) revolution in the process, the outer shaft sleeve (3) through the transmission gear (4) drive upper gear ring (12) rotation, upper gear ring (12) rotation, in turn, make the main rotating seat (8) revolution at the same time occur at a set speed of rotation, the main rotating seat (8) rotation, due to the driven gear (14) and the lower gear ring (11) meshing connection settings, placed rotating cylinder (13) in the main rotating seat (8) rotation, also occur synchronous rotation, through the rotation of the above-mentioned, thus each incubation tube (15) are uniformly cold, and in the work cycle of the servo motor (6), the electromagnetic ring (20) periodically energized, in the energized cycle of the electromagnetic ring (20), electromagnetic ring (20) and the permanent magnet ring (24) between the repulsion, repulsion after, thus drive the excitation cylinder (21) upward movement, through the energized cycle control of the electromagnetic ring (20), can effectively control the excitation cylinder (21) vibration frequency, through the electromagnetic ring (20) of the current control, can effectively control the excitation cylinder (21) of the displacement stroke, excitation cylinder (21) vibration, in turn, through the support ring (22) acting on the incubation tube (15), support ring (22) acting on the incubation tube (15), in turn, make the incubation tube (15) up and down excitation, when you need to take out the incubation tube (15), open the door (2), in turn, can complete the storage of the incubation tube (15) take out the operation.

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