A kind of fixing device and fixing method of female mouse

Abstract

The present invention discloses a fixing device and fixing method for female mice, belonging to the technical field of mouse mating experiments. The fixing device comprises a fixing seat and a limiting plate, the fixing seat comprises a bottom plate, a support plate and a limiting frame, one end of the support plate is rotatably connected to the middle part of the bottom plate, and the inclination angle a of the support plate relative to the bottom plate is configured to be adjustable, the support plate is used to support the buttocks of female mice, the limiting frame is fixed on the bottom plate and is located on the front side of the support plate, the limiting plate is fixed on the head of the female mice, the limiting plate and the limiting frame are detachably connected, and a limiting space for limiting the head of the female mice can be formed between the limiting plate, the limiting frame and the bottom plate. The fixing device can not only lift the buttocks of female mice, so that the female mice are in a posture that is conducive to mating with male mice, which is conducive to improving the experimental efficiency and shortening the experimental time; and can reduce the number of times the female mice are anesthetized, which is conducive to avoiding damage to the female mice caused by multiple anesthesia.

Description

Technical Field

[0001] The invention relates to the technical field of mouse mating experiments, and in particular to a fixing device and a fixing method for female mice. Background Art

[0002] Mice are one of the most commonly used living experimental subjects in various experiments, such as courtship, mating, sperm allocation, pregnancy and fertility. However, under natural conditions, mating behavior is determined by both males and females, and it is difficult to distinguish the roles of each. When obtaining semen through natural mating, if a female mouse intends to mate, it will lower its chest to the floor and raise its vaginal opening, inviting the male mouse to mate in this posture. When a female mouse refuses to mate, it will have many forms of refusal, such as arching its back to hide its vaginal opening, running away, and directly fighting with the male mouse.

[0003] Since the mating intention of female mice is uncontrollable, the probability of female mice refusing to mate is high when conducting experiments to obtain semen through natural mating, which in turn leads to excessive experimental time, more energy consumed by experimenters, excessive influence of females on experimental results, and inability to analyze male factors alone. Therefore, in existing experiments, female mice are generally fixed with a fixing device to reduce the influence of female mice on male mice and the interaction between the two sexes.

[0004] However, the existing fixing device is relatively simple, which includes an ear grabbing mechanism and a tooth tray mechanism at a certain distance, and the ear grabbing mechanism and the tooth tray mechanism are used to fix the ears and mouth of the female mouse respectively, so as to directly fix the head of the female mouse. The existing fixing device cannot lift the vaginal opening of the female mouse, so that the female mouse cannot be adjusted to a posture conducive to mating. In addition, since the existing fixing device will cause severe pain to the female mouse, the female mouse needs to be anesthetized, and since the female mouse needs to participate in multiple mating experiments, the female mouse needs to be anesthetized multiple times in a short period of time. Multiple anesthesia will not only seriously affect the state of the female mouse, thereby preventing the female mouse from being in the correct mating posture, but may even accidentally kill the female mouse.

[0005] Therefore, how to propose a device and method that can fix female mice to make them present a mating posture and can prevent female mice from being anesthetized multiple times is a technical problem that urgently needs to be solved. Summary of the invention

[0006] One purpose of the present invention is to provide a fixing device for female mice, which can not only fix and adjust the experimental posture of female mice, but also help to reduce the time required for the experiment, improve the experimental efficiency, and reduce the damage of the experiment to female mice.

[0007] To achieve this object, the present invention adopts the following technical solutions:

[0008] A fixing device for female mice, comprising:

[0009] A fixing seat, the fixing seat comprising a bottom plate, a support plate and a limiting frame, one end of the support plate is rotatably connected to the middle of the bottom plate, and the inclination angle a of the support plate relative to the bottom plate is configured to be adjustable, the support plate is used to support the buttocks of a female mouse, and the limiting frame is fixed to the bottom plate and is located at the front side of the support plate;

[0010] A limiting plate is fixed on the head of the female mouse, the limiting plate is detachably connected to the limiting frame, and a limiting space for limiting the head of the female mouse can be formed between the limiting plate, the limiting frame and the bottom plate.

[0011] Preferably, two rotating shaft seats are convexly provided at intervals along the first direction in the middle of the bottom plate, each rotating shaft seat is provided with a first threaded through hole along the first direction, the rotating end of the support plate is placed between the two rotating shaft seats, and a second threaded through hole is provided on the rotating end of the support plate along the first direction;

[0012] The fixing device for the female mouse also includes two fastening screws, which pass through the first threaded through holes of the two rotating shaft seats one by one and are then tightened at both ends of the second threaded through holes.

[0013] Preferably, the limiting frame comprises two limiting rods, the two limiting rods are arranged at intervals along the first direction, and the two limiting rods are detachably connected to two ends of the limiting plate.

[0014] Preferably, the limit rod comprises a vertical rod, a horizontal rod and a locking knob, the bottom end of the vertical rod is detachably connected to the bottom plate, one end of the horizontal rod is rotatably connected to the top of the vertical rod, and the other end of the horizontal rod is provided with a third threaded through hole in the vertical direction;

[0015] The two ends of the limit plate are respectively provided with limit slots, and the threaded rod of the locking knob passes through the limit slots and is threadedly connected in the third threaded through hole.

[0016] Preferably, the limiting groove is a U-shaped groove.

[0017] Preferably, a glue coating hole is provided in the middle of the limiting plate.

[0018] Preferably, the limiting plate includes a first connecting part, a second connecting part and a third connecting part which are arranged in sequence along the first direction, the width of the second connecting part along the second direction is greater than the width of the first connecting part and the width of the third connecting part, the glue coating hole is opened through the second connecting part, the two limiting grooves are respectively opened on the first connecting part and the third connecting part, and the second direction is perpendicular to the first direction.

[0019] Another object of the present invention is to provide a method for fixing female mice, which is beneficial to improving experimental efficiency, shortening the time required for the experiment, and can avoid female mice being anesthetized multiple times, thereby reducing the damage to female mice caused by the experiment.

[0020] To achieve this object, the present invention adopts the following technical solutions:

[0021] A method for fixing female mice, using the above-mentioned fixing device for female mice, the method for fixing female mice specifically comprises the following steps:

[0022] S1. Fix the middle part of the restraining plate on the head of the female mouse;

[0023] S2, placing the hip of the female mouse with the limiting plate on the support plate of the fixing seat, and placing the head of the female mouse on the bottom plate of the fixing seat;

[0024] S3, connecting and fixing the limiting plate and the limiting frame to limit the head of the female mouse within the limiting space formed by the limiting plate, the limiting frame and the bottom plate;

[0025] S4. Adjust the inclination angle a of the support plate relative to the base plate to a target value.

[0026] Preferably, the step of connecting and fixing the limiting plate and the limiting frame in S3 specifically comprises the following steps:

[0027] S31, fixing the vertical rods of the two limiting rods included in the limiting frame to the bottom plate;

[0028] S32, rotating the horizontal rod of the limiting rod around the vertical rod so that the third threaded through hole on the horizontal rod is located directly above the limiting groove of the limiting plate;

[0029] S33, passing the threaded rod of the locking knob of the limiting rod through the limiting groove and then tightening it into the third threaded through hole.

[0030] Preferably, the S4 specifically includes the following steps:

[0031] S41, screwing down a fastening screw from both ends of the second threaded through hole of the support plate respectively;

[0032] S42, adjusting the inclination angle a of the support plate relative to the base plate to a target value;

[0033] S43, passing the two fastening screws through the first threaded through holes on the two rotating shaft seats in the middle of the base plate one by one, and then tightening them again at the two ends of the second threaded through holes.

[0034] Beneficial effects of the present invention:

[0035] The present invention provides a fixing device for female mice, the fixing device comprises a fixing seat and a limiting plate, the fixing seat comprises a bottom plate, a support plate and a limiting frame, one end of the support plate is rotatably connected to the middle part of the bottom plate, and the inclination angle a of the support plate relative to the bottom plate is configured to be adjustable, the support plate is used to support the buttocks of the female mice, the limiting frame is fixed on the bottom plate and is located at the front side of the support plate, the limiting plate is fixed on the head of the female mice, the limiting plate and the limiting frame are detachably connected, and a limiting space for limiting the head of the female mice can be formed between the limiting plate, the limiting frame and the bottom plate. The fixing device can lift the buttocks of the female mice by setting a supporting plate with a variable inclination angle compared to the bottom plate, so that the female mice are in a posture that is conducive to mating with male mice, which is conducive to improving the experimental efficiency and shortening the experimental time; and by fixing the limiting plate on the head of the mouse, only the limiting plate and the limiting frame need to be disassembled and assembled during the experiment, which reduces the number of times the female mice need to be anesthetized, which is conducive to avoiding the damage of the female mice caused by multiple anesthesia. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 is a top view of a fixing seat of a fixing device for female mice provided in an embodiment of the present invention;

[0037] Figure 2 is a front view of a fixing seat of a fixing device for female mice provided in an embodiment of the present invention;

[0038] Figure 3 is a side view of a fixing seat of a fixing device for female mice provided in an embodiment of the present invention;

[0039] Figure 4 4 is a top view of a limiting plate of a fixing device for female mice provided in an embodiment of the present invention.

[0040] In the figure:

[0041] 100, fixed seat;

[0042] 110, bottom plate; 120, support plate; 130, limit rod; 131, vertical rod; 132, horizontal rod; 133, locking knob; 140, rotating shaft seat; 150, fastening screw;

[0043] 200, limit plate;

[0044] 210, limiting groove; 220, glue coating hole. DETAILED DESCRIPTION

[0045] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0046] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance. Among them, the terms "first position" and "second position" are two different positions.

[0047] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0048] The present invention provides a fixing device for female mice (hereinafter referred to as the fixing device), which can be used in mating experiments between female mice and male mice. The fixing device can fix the female mice and adjust the posture of the female mice so that the female mice present a posture with the head down and the hips up that is suitable for mating with male mice.

[0049] Specifically, Figures 1 to 4As shown, the fixing device includes a fixing seat 100 and a limiting plate 200. Among them, the fixing seat 100 is the main structure of the fixing device, which specifically includes a base plate 110, a support plate 120 and a limiting frame. In the present embodiment, the base plate 110 is a rectangular plate-like structure, which can be a whole plate or formed by splicing and fixing multiple plates. The length of the base plate 110 is 132mm, the width is 65mm, and the height is 0.8mm. The support plate 120 is used to support the buttocks of female mice, and one end of the support plate 120 is rotatably connected to the middle part of the base plate 110, and the inclination angle a of the support plate 120 relative to the base plate 110 is configured to be adjustable. In the present embodiment, the length of the support plate 120 is 65mm and the width is 25mm. Optionally, the adjustment range of the inclination angle a is 0°-90°, for example, it can be 15°, 25°, 35°, 50°, 70°, or 89°.

[0050] The limiting frame is used to assist in fixing the head of the female mouse. The limiting frame is fixed on the base plate 110 and is located on the front side of the support plate 120. The front side here refers to the position of the female mouse placed on the base plate 110 as a reference. The limiting frame is located on the side close to the head of the female mouse, and the support plate 120 is located on the side close to the buttocks of the female mouse. The limiting plate 200 is used to assist in fixing the head of the female mouse, and the limiting plate 200 and the limiting frame can be used in conjunction. Specifically, the limiting plate 200 is fixed on the head of the female mouse, and the limiting plate 200 is detachably connected to the limiting frame, and a limiting space for limiting the head of the female mouse can be formed between the limiting plate 200, the limiting frame and the base plate 110.

[0051] The fixing device is provided with a support plate 120 with a variable inclination angle compared to the bottom plate 110, so that the buttocks of the female mouse can be lifted by the support plate 120, so that the female mouse is in a posture with the head down and the buttocks up, which is conducive to mating with the male mouse, so as to simulate natural mating. The use of the fixing device limits the rejection behavior of the female mouse without the intention of mating, so that the female mouse cannot hide its vaginal opening, and the vaginal opening is forced to be exposed, which is conducive to reducing the impact of the female mouse's lack of mating intention on the experiment, and is conducive to improving the efficiency of the experiment and shortening the experimental time.

[0052] Furthermore, since the limiting plate 200 does not need to be disassembled after being fixed on the head of the female mouse, when the female mouse needs to conduct multiple experiments in a short period of time, it is only necessary to disassemble and assemble the limiting plate 200 and the limiting frame. Compared with the repeatedly worn fixing device used in the prior art, in order to reduce the pain of wearing the fixing device, the female mouse needs to be anesthetized for each experiment. The fixing device in this embodiment directly fixes the limiting plate 200 on the head of the female mouse. During the experiment, only the limiting plate 200 needs to be disassembled and assembled, and no additional operation is required for the female mouse, thereby reducing the number of times the female mouse needs to be anesthetized and avoiding damage to the female mouse caused by multiple anesthesia.

[0053] Optionally, continue to refer to Figures 1 to 3 As shown, two rotating shaft seats 140 are convexly arranged at intervals along the first direction in the middle of the bottom plate 110, and the two rotating shaft seats 140 are respectively the first rotating shaft seat and the second rotating shaft seat, and a first threaded through hole is respectively arranged on the first rotating shaft seat and the second rotating shaft seat along the first direction. The support plate 120 includes two ends, a rotating end and an adjusting end, and the rotating end of the support plate 120 is placed between the first rotating shaft seat and the second rotating shaft seat, and a second threaded through hole is opened on the rotating end of the support plate 120 along the first direction. Further optionally, the width of the rotating end along the first direction is smaller than that of the end of the adjusting end, so that the rotating end is easier to be placed between the first rotating shaft seat and the second rotating shaft seat.

[0054] Continue to refer to Figures 1 to 3 As shown, the fixing device of the female mouse also includes two fastening screws 150, namely, a first fastening screw and a second fastening screw. The first fastening screw passes through the first threaded through hole on the first rotating shaft seat and is tightened at one end of the second threaded through hole. The second fastening screw passes through the first threaded through hole of the second rotating shaft seat and is tightened at the other end of the second threaded through hole. When it is necessary to adjust the inclination angle a of the support plate 120 relative to the base plate 110, it is only necessary to completely unscrew the first fastening screw and the second fastening screw from both ends of the second threaded through hole. At this time, the inclination angle a of the support plate 120 relative to the base plate 110 can be adjusted by applying force to the adjustment end of the support plate 120. When the inclination angle a is adjusted, it is only necessary to re-tighten the first fastening screw and the second fastening screw at both ends of the second threaded through hole. The structure for adjusting the inclination angle a of the support plate 120 is compact, the method is simple, and the cost is low.

[0055] Optionally, the limiting frame includes two limiting rods 130, the two limiting rods 130 are spaced apart along the first direction, and the two limiting rods 130 are detachably connected to both ends of the limiting plate 200. Figure 2 and Figure 3 As shown, each limiting rod 130 includes a vertical rod 131, a horizontal rod 132 and a locking knob 133. A threaded connection hole is provided on the bottom plate 110, and a stud is provided at the bottom end of the vertical rod 131. The stud is threadedly connected in the threaded connection hole, so that the vertical rod 131 can be detachably connected to the bottom plate 110. Of course, in addition to the threaded connection, other detachable connection methods are also applicable to the bottom plate 110 and the vertical rod 131 in this embodiment. The detachable connection between the limiting frame and the bottom plate 110 makes the entire fixing device easy to carry.

[0056] One end of the horizontal rod 132 is rotatably connected to the top of the vertical rod 131, and the other end of the horizontal rod 132 is provided with a third threaded through hole in the vertical direction. The two ends of the limit plate 200 are respectively provided with a limit groove 210, and the threaded rod of the locking knob 133 is threadedly connected in the third threaded through hole after passing through the limit groove 210. The knob of the locking knob 133 and the horizontal rod 132 clamp the limit plate 200 from the upper and lower sides of the limit plate 200, so that the connection between the limit frame and the limit plate 200 can be realized. This structure is simple to assemble and relatively stable, and can realize the stable limiting of female mice. Of course, in other embodiments, the vertical rod 131 can also be rotatably connected to the base plate 110, and the horizontal rod 132 can be fixedly connected to the vertical rod 131, so as to realize the rotation connection between the limit rod 130 and the base plate 110.

[0057] Optionally, the limiting groove 210 is a U-shaped groove, so that the limiting plate 200 can be inserted between the knob of the locking knob 133 and the horizontal rod 132 from the side opening of the U-shaped groove, thereby reducing the difficulty of assembly. Of course, other shapes of limiting grooves 210 are also applicable to this embodiment, such as rectangular grooves, waist-shaped grooves, etc.

[0058] Optionally, a glue hole 220 is provided in the middle of the limiting plate 200. The limiting plate 200 can be bonded to the head of the female mouse by placing glue in the glue hole 220. In this way, the bonding strength is high, and the limiting plate 200 is not easy to fall off from the head of the female mouse.

[0059] Further optionally, the limiting plate 200 includes a first connection portion, a second connection portion, and a third connection portion sequentially arranged along the first direction, the width of the second connection portion along the second direction is greater than the width of the first connection portion and the width of the third connection portion, the glue hole 220 is opened through the second connection portion, and the two limiting grooves 210 are respectively opened on the first connection portion and the third connection portion, and the second direction is perpendicular to the first direction. Such an arrangement can not only improve the aesthetics of the limiting plate 200 and make it more suitable for the head of the female mouse, but also appropriately expand the size of the glue hole 220, thereby further improving the strength of the adhesive.

[0060] This embodiment also provides a method for fixing female mice using the above-mentioned fixing device. The fixing method is beneficial to improving experimental efficiency, shortening the time required for the experiment, and can avoid female mice being anesthetized multiple times, thereby reducing the damage to female mice caused by the experiment.

[0061] Specifically, the fixation method of the female mouse comprises the following steps:

[0062] S1, fixing the middle part of the limiting plate 200 on the head of the female mouse;

[0063] S2, placing the hip of the female mouse with the limiting plate 200 on the support plate 120 of the fixing seat 100, and placing the head of the female mouse on the bottom plate 110 of the fixing seat 100;

[0064] S3, connecting and fixing the limiting plate 200 and the limiting frame to limit the head of the female mouse in the limiting space formed by the limiting plate 200, the limiting frame and the bottom plate 110;

[0065] S4. Adjust the inclination angle a of the support plate 120 relative to the bottom plate 110 to a target value.

[0066] Optionally, in the above step S1, first, anesthetize the female mouse with 50 mg / kg of 1% sodium pentobarbital, remove the hair on the head of the female mouse, and remove a circular skin area with a diameter of about 1 cm; then, use medical hydrogen peroxide to clean the skull of the female mouse and remove tissue, use a cranial drill to make the skull rougher, and after the bleeding of the head of the female mouse stops, drop a drop of 406 glue on the skull to form a thin layer of glue, and after the first layer of glue dries, spread a layer of 5400 glue on it to form a second layer of glue; then, bond the metal limiting plate 200 to the second layer of glue; finally, mix 5400 glue and dental powder and apply it to the glue hole 220 on the limiting plate 200 until the glue hole 220 is filled, and the same mixture is also applied to the edge of the limiting plate 200 that contacts the skin of the female mouse.

[0067] Optionally, the step S3 of connecting and fixing the limiting plate 200 and the limiting frame specifically includes the following steps:

[0068] S31, fixing the vertical rods 131 of the two limiting rods 130 included in the limiting frame on the bottom plate 110;

[0069] S32, rotating the horizontal rod 132 of the limiting rod 130 around the vertical rod 131, so that the third threaded through hole on the horizontal rod 132 is located directly above the limiting groove 210 of the limiting plate 200;

[0070] S33, passing the threaded rod of the locking knob 133 of the limiting rod 130 through the limiting groove 210 and then tightening it into the third threaded through hole.

[0071] Optionally, the above step S4 specifically includes the following steps:

[0072] S41, screwing down a fastening screw 150 from both ends of the second threaded through hole of the support plate 120;

[0073] S42, adjusting the inclination angle a of the support plate 120 relative to the base plate 110 to a target value;

[0074] S43, passing two fastening screws 150 through the first threaded through holes on the two rotating shaft seats 140 disposed in the middle of the bottom plate 110 one by one, and then tightening them again at the two ends of the second threaded through holes.

[0075] Optionally, after the above step S4, the following steps are also included:

[0076] S5. Place the freely moving male mouse into the cage of the fixed female mouse, so that the female mouse and the male mouse can mate.

[0077] Specifically, first, the male mice will check the adapted fixed female mice and the above-mentioned fixing device, and the male mice will soon get used to the appearance of the female mice and the above-mentioned fixing device. After multiple rounds of training, the male mice will be familiar with the fixed position of the female mice and the above-mentioned fixing device, and the male mice can easily complete mating. After training, about 10% of male mice can get used to the fixing device and successfully ejaculate, and the first sniffing of genitals, riding on the back, and thrusting usually occur within an hour, which is much shorter than the natural mating time.

[0078] In addition, the fixation device can be used in conjunction with female condoms for mice. In current experiments, female mice need to be killed to collect sperm. The use of female condoms enables male mice to thrust in and out of the condoms. Fixed female mice are more difficult to expel condoms than freely moving mice, so it is easier to collect sperm. Moreover, female condoms can collect clean semen without being interfered by the body fluids of female mice. Clean semen is ideal for proteomic analysis. Most importantly, we do not need to sacrifice female mice, and female mice can be used multiple times, which is suitable for more complex experimental designs.

[0079] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the embodiments here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A fixing device for female mice, characterized in that: include: A fixing seat (100), the fixing seat (100) comprising a bottom plate (110), a support plate (120) and a limiting frame, one end of the support plate (120) being rotatably connected to the middle portion of the bottom plate (110), and an inclination angle a of the support plate (120) relative to the bottom plate (110) being configured to be adjustable, the support plate (120) being used to support the buttocks of a female mouse, and the limiting frame being fixed to the bottom plate (110) and located at the front side of the support plate (120); a limiting plate (200), wherein the limiting plate (200) is fixed to the head of the female mouse, the limiting plate (200) is detachably connected to the limiting frame, and a limiting space for limiting the head of the female mouse can be formed between the limiting plate (200), the limiting frame and the bottom plate (110); The limiting frame, the limiting plate (200) and the support plate (120) with adjustable angle are used in cooperation to place the female mouse in a mating posture with the head down and the hips up; The limiting frame comprises two limiting rods (130), the two limiting rods (130) are arranged at intervals along the first direction, and the two limiting rods (130) are respectively detachably connected to two ends of the limiting plate (200); The limiting rod (130) comprises a vertical rod (131), a horizontal rod (132) and a locking knob (133); the bottom end of the vertical rod (131) is detachably connected to the bottom plate (110); one end of the horizontal rod (132) is rotatably connected to the top of the vertical rod (131); a third threaded through hole is provided on the other end of the horizontal rod (132) in the vertical direction; limiting grooves (210) are respectively provided at both ends of the limiting plate (200); and the threaded rod of the locking knob (133) passes through the limiting groove (210) and is threadedly connected to the third threaded through hole.

2. The fixing device for female mice according to claim 1, characterized in that: Two rotating shaft seats (140) are protruded at intervals along a first direction in the middle of the bottom plate (110), each of the rotating shaft seats (140) having a first threaded through hole extending along the first direction, the rotating end of the support plate (120) is placed between the two rotating shaft seats (140), and a second threaded through hole is opened along the first direction on the rotating end of the support plate (120); The female mouse fixing device further comprises two fastening screws (150), the two fastening screws (150) correspondingly passing through the first threaded through holes of the two rotating shaft seats (140) one by one and then being tightened at two ends of the second threaded through holes.

3. The fixing device for female mice according to claim 1, characterized in that: The limiting groove (210) is a U-shaped groove.

4. The fixing device for female mice according to claim 1, characterized in that: A glue coating hole (220) is provided in the middle of the limiting plate (200).

5. The fixing device for female mice according to claim 4, characterized in that: The limiting plate (200) comprises a first connection portion, a second connection portion and a third connection portion which are sequentially arranged along a first direction, the width of the second connection portion along the second direction is greater than the width of the first connection portion and the width of the third connection portion, the glue coating hole (220) is provided through the second connection portion, the two limiting grooves (210) are respectively provided on the first connection portion and the third connection portion, and the second direction is perpendicular to the first direction.

6. A method for fixing female mice, characterized in that: Using the female mouse fixing device according to any one of claims 1 to 5, the female mouse fixing method specifically comprises the following steps: S1. Fixing the middle part of the limiting plate (200) on the head of the female mouse; S2, placing the buttocks of the female mouse with the limiting plate (200) on the support plate (120) of the fixing seat (100), and placing the head of the female mouse on the bottom plate (110) of the fixing seat (100); S3, connecting and fixing the limiting plate (200) and the limiting frame, so as to limit the head of the female mouse within the limiting space formed by the limiting plate (200), the limiting frame and the bottom plate (110); S4. Adjusting the inclination angle a of the support plate (120) relative to the bottom plate (110) to a target value.

7. The method for fixing female mice according to claim 6, characterized in that: The step of connecting and fixing the limiting plate (200) and the limiting frame in S3 specifically comprises the following steps: S31, fixing the vertical rods (131) of the two limiting rods (130) included in the limiting frame onto the bottom plate (110); S32, rotating the horizontal rod (132) of the limiting rod (130) around the vertical rod (131) so that the third threaded through hole on the horizontal rod (132) is located directly above the limiting groove (210) of the limiting plate (200); S33, passing the threaded rod of the locking knob (133) of the limiting rod (130) through the limiting groove (210) and then tightening it into the third threaded through hole.

8. The method for fixing female mice according to claim 6, characterized in that: The S4 specifically includes the following steps: S41, screwing a fastening screw (150) from each of the two ends of the second threaded through hole of the support plate (120); S42, adjusting the inclination angle a of the support plate (120) relative to the bottom plate (110) to a target value; S43, passing the two fastening screws (150) through the first threaded through holes on the two rotating shaft seats (140) disposed in the middle of the bottom plate (110) one by one, and then tightening them again at the two ends of the second threaded through holes.

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