A fully automatic liquid-based cell slide dyeing and sealing system and its control method
Through the design of the fully automatic liquid-based cell production and staining system, the problem of cross-contamination and long-term sampling time in the prior art is solved, and efficient cell production automation is achieved, ensuring production quality and efficiency.
Patent Information
- Application Number
- CN202211055797.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-08-31
- Publication Date
- 2025-01-03
- Estimated Expiration
- 2042-08-31
AI Technical Summary
In the existing natural sedimentation cell production technology, the steel needles used for sampling are cross-contaminated, and the staining and sealing process time is too long, resulting in the cell layer being exposed to the air and atrophy, affecting the production quality.
A fully automatic liquid-based cell production and dyeing system is designed, including a shelf platform, sample storage mechanism, circulation conveying mechanism, glass slide loading mechanism, settlement chamber loading mechanism, sample loading mechanism, coding mechanism, and liquid addition The mechanism, settlement bin cutting mechanism and sealing mechanism ensure wet sealing through automated processes, avoid dry sealing, and improve production quality.
The degree of automation of production is improved, the quality of cell production is ensured, cross-contamination and cell atrophy is avoided, and the efficiency and consistency of production is improved.
Smart Images

Figure CN115583503B_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of cell slide making, and in particular relates to a full-automatic liquid-based cell slide making staining and sealing system and a control method thereof. Background Art
[0002] The basic principle of natural sedimentation cell preparation technology is to use gradient separation and liquid-based technology to transfer representative cells to a glass slide by natural sedimentation, and then stain them to form a cell sheet with natural cell morphology, uniform distribution, single-layer tiling, clean background, distinct color, and clear structure, so that researchers can observe and study clearly. In the existing technology, sampling uses recycled steel needles, which may cause cross contamination; the connection time between the staining and sealing processes is too long, and the cell layer is exposed to the air, causing cell atrophy and affecting the quality of preparation. Summary of the invention
[0003] In order to solve the problems in the related art, the present application provides a fully automatic liquid-based cell slide dyeing and sealing system and a control method thereof, which can improve the degree of automation of slide production and effectively improve the quality of slide production.
[0004] The technical solution is as follows:
[0005] A fully automatic liquid-based cell slide staining and sealing system comprises an upper platform, a sample storage mechanism, a lower platform, a circulating conveying mechanism, a glass slide loading mechanism, a sedimentation bin loading mechanism, a sample loading mechanism, a coding mechanism, a liquid adding mechanism, a sedimentation bin unloading mechanism, a sealing mechanism and a film collecting mechanism, wherein: the upper platform is arranged above the lower platform; the circulating conveying mechanism, the glass slide loading mechanism, the sedimentation bin loading mechanism, the sample loading mechanism, the coding mechanism, the liquid adding mechanism, the sedimentation bin unloading mechanism, the sealing mechanism and the film collecting mechanism are all installed on the upper platform; the glass slide loading mechanism, the sedimentation bin loading mechanism, the sample loading mechanism, the coding mechanism, the liquid adding mechanism, the sedimentation bin unloading mechanism, the sealing mechanism and the film collecting mechanism are sequentially arranged on the side of the circulating conveying mechanism along the conveying direction of the circulating conveying mechanism; the sample storage mechanism is installed on the lower platform; and the sample loading mechanism is used to transfer the sample of the sample storage mechanism to the sedimentation bin on the circulating conveying mechanism.
[0006] The processes corresponding to the liquid adding mechanism and the sealing mechanism are connected and set to ensure wet sealing of the slices and avoid dry sealing, thus ensuring the quality of the slices.
[0007] Furthermore, the lower platform is provided with a transfer rail frame, and a plurality of fixing clips are slidably provided on the track of the transfer rail frame, and the bottom of the sample storage mechanism is installed on the fixing clips; the transfer rail frame can carry at least two groups of sample storage mechanisms at the same time.
[0008] The sample storage mechanism can be moved on the transfer rail frame after being fixed by a fixing clamp, which is conducive to rapid sample supply.
[0009] Furthermore, the sample storage mechanism includes a syringe storage area and a sample storage area. The syringe storage area stores sampling syringes, and the sample storage area stores sample bottles. The sampling syringes correspond to the sample bottles one by one.
[0010] The sampling syringes are matched one by one with the sample bottles, and the sampling syringes are disposable to ensure that the sample bottles will not be contaminated at all.
[0011] Furthermore, the sample loading mechanism is provided with a mechanical clamp, which can take samples from the sample bottle with a sampling syringe and then add the sample in the sampling syringe into a sedimentation bin of the sample loading station of the circulation conveying mechanism.
[0012] Furthermore, the sampling syringe includes a sampling barrel, a needle tube and a piston push rod, wherein: the needle tube is installed at the front end of the sampling barrel; the needle tube is connected to the interior of the sampling barrel; the piston end of the piston push rod is inserted into the rear end of the sampling barrel; the piston end of the piston push rod can perform piston movement inside the sampling barrel; a ring platform is provided on the circumference of the sampling barrel; the outer diameter of the ring platform is larger than the aperture size of the syringe plug hole; the needle tube is used to be inserted into the sample bottle to connect the sample bottle with the interior of the sampling barrel; the piston push rod is used to create negative pressure inside the sampling barrel.
[0013] Furthermore, the sample storage area is provided with a first frame plate and a second frame plate, the first frame plate is arranged parallel to the second frame plate, the first frame plate is provided with a placement hole, and the second frame plate is provided with a lifting hole corresponding to the placement hole one by one; the aperture of the placement hole is larger than the diameter of the sample bottle, and the aperture of the lifting hole is smaller than the diameter of the sample bottle, and the sample bottle is inserted into the placement hole and placed on the lifting hole; the sample storage mechanism also includes a lifting assembly, a rotating assembly and a scanning assembly; the rotating assembly is installed on the lifting end of the lifting assembly; the rotating part of the rotating assembly is arranged below the lifting hole; the rotating part of the rotating assembly can pass through the lifting hole to contact the bottom of the sample bottle; the scanning end of the scanning assembly corresponds to the side of the sample bottle.
[0014] The sample bottle is rotated by the rotating component until the code is scanned successfully. There is no need to place the sample bottle in a fixed posture, which facilitates the quick placement of the sample bottle.
[0015] Furthermore, the sedimentation bin feeding mechanism includes a feeding clamp, a temporary storage structure, a material transfer clamp and a sorting structure, wherein: the feeding clamp is arranged at the rear material storage channel of the temporary storage structure; the material transfer clamp is arranged at the side of the temporary storage structure; the sorting structure is arranged at the front material storage channel of the temporary storage structure; the feeding clamp is used to transfer the sedimentation bin of the temporary storage structure to the circulating conveying mechanism; the material transfer clamp is used to transfer the sedimentation bin sorted by the sorting structure to the temporary storage structure; the sorting structure is used to organize the scattered sedimentation bins into sedimentation bins with fixed postures.
[0016] By setting the sorting structure, the sedimentation bin can be sorted automatically without manual adjustment of the sedimentation bin posture, and it can run automatically for a long time.
[0017] Furthermore, the sorting structure includes a material accumulation box and a push rod, wherein: the material accumulation box is a funnel structure; the top end of the push rod passes through the bottom of the material accumulation box; the top end of the push rod corresponds to the inner hole of the sedimentation bin, and the bottom end of the push rod is provided with a driving device; the driving device is used to drive the push rod to push upward from the bottom of the material accumulation box.
[0018] Furthermore, the circulating conveying mechanism adopts an integrated circulating conveying structure.
[0019] The circulating conveying mechanism can be set up to make the circulating conveying integrated and avoid jamming failures.
[0020] A control method for a fully automatic liquid-based cell slide dyeing and sealing system comprises the following steps:
[0021] Step 1: The lifting component lifts up the rotating component, and the rotating component lifts the sample bottle to the scanning area of the code scanning component. The rotating component adjusts the direction of the information label on the side of the sample bottle by rotation, so that the code scanning component obtains the sample information;
[0022] Step 2: The slide loading mechanism loads the corresponding slide onto the circulation conveying mechanism according to the sample information;
[0023] Step 3, when the glass slide is transported to the station where the sedimentation bin loading mechanism is located, the sedimentation bin loading mechanism loads the sedimentation bin onto the glass slide;
[0024] Step 4: When the glass slide and the sedimentation chamber are transported to the station where the sample loading mechanism is located, the mechanical gripper clamps the sampling syringe to extract the sample in the corresponding sample bottle, and then adds the extracted sample into the sedimentation chamber;
[0025] Step 5, when the glass slide and the sedimentation bin are transported to the station where the coding mechanism is located, the coding mechanism codes the sedimentation bin;
[0026] Step six, after the slide and the sample in the sedimentation chamber have been settled, they are transported to the station where the liquid adding mechanism is located. The liquid adding mechanism first fixes the cleaning liquid in the sedimentation chamber, then washes it with the buffer solution, and adds the hematoxylin dye solution. After the first step of staining is completed, the buffer solution is washed and the EA dye solution is added. After the second step of staining is completed, the cleaning liquid is added for washing, and then the transparent liquid is added to complete the staining and preparation.
[0027] Step 7, when the glass slide and the sedimentation bin are transported to the station where the sedimentation bin unloading mechanism is located, the sedimentation bin unloading mechanism takes away the sedimentation bin and discards it;
[0028] Step eight, when the slide is transported to the workstation where the sealing mechanism is located, the sealing mechanism takes the slide, adds sealing glue, adsorbs the cover glass, seals the slide, and then puts the sealed slide into the slide collection box.
[0029] It is to be understood that the foregoing general description and the following detailed description are exemplary only and are not restrictive of the invention. BRIEF DESCRIPTION OF THE DRAWINGS
[0030] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and, together with the description, serve to explain the principles of the invention.
[0031] Figure 1 It is a schematic diagram of the overall structure of the present invention;
[0032] Figure 2 It is a schematic diagram of the top view of the structure of the upper platform of the present invention;
[0033] Figure 3 It is a schematic diagram of the coordination relationship between the lifting assembly, the rotating assembly and the code scanning assembly of the present invention;
[0034] Figure 4 It is a schematic structural diagram of the first frame plate and the second frame plate of the present invention;
[0035] Figure 5 It is a structural schematic diagram of the sampling syringe of the present invention;
[0036] Figure 6 It is a schematic diagram of the top view of the structure of the sedimentation bin unloading mechanism of the present invention;
[0037] Figure 7 It is a side structural schematic diagram of the sedimentation bin unloading mechanism of the present invention;
[0038] Figure 1-7 Including: 11. upper platform; 2. sample storage mechanism; 27. lifting assembly; 25. rotating assembly; 26. code scanning assembly; 21. sampling syringe; 211. sampling tube; 212. needle tube; 213. piston push rod; 22. sample bottle; 23. first shelf; 24. second shelf; 12. lower platform; 121. transfer rail; 122. fixing clamp; 3. circulating conveying mechanism; 4. slide loading mechanism; 5. sedimentation bin loading mechanism; 51. loading claw; 52. temporary storage structure; 53. material transfer claw; 54. sorting structure; 541. material accumulation box; 542. ejector; 6. sample loading mechanism; 8. liquid adding mechanism; 9. sedimentation bin unloading mechanism; 10. sealing mechanism. DETAILED DESCRIPTION
[0039] Exemplary embodiments will be described in detail herein, examples of which are shown in the accompanying drawings. When the following description refers to the drawings, the same numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present invention. Instead, they are merely examples of devices and methods consistent with some aspects of the present invention as detailed in the appended claims.
[0040] As attached Figure 1-7 As shown:
[0041] A fully automatic liquid-based cell slide dyeing and sealing system comprises an upper platform 11, a sample storage mechanism 2, a lower platform 12, a circulating conveying mechanism 3, a glass slide loading mechanism 4, a sedimentation bin loading mechanism 5, a sample loading mechanism 6, a coding mechanism, a liquid adding mechanism 8, a sedimentation bin unloading mechanism 9, a sealing mechanism 10 and a film collecting mechanism, wherein: the upper platform 11 is set above the lower platform 12; the circulating conveying mechanism 3, the glass slide loading mechanism 4, the sedimentation bin loading mechanism 5, the sample loading mechanism 6, the coding mechanism, the liquid adding mechanism 8, the sedimentation bin unloading mechanism 9, the sealing mechanism 10 and the film collecting mechanism. The lowering bin unloading mechanism 9, the sealing mechanism 10 and the collecting mechanism are all installed on the upper platform 11; the slide loading mechanism 4, the sedimentation bin loading mechanism 5, the sample loading mechanism 6, the coding mechanism, the liquid adding mechanism 8, the sedimentation bin unloading mechanism 9, the sealing mechanism 10 and the collecting mechanism are arranged in sequence on the side of the circulating conveying mechanism 3 along the conveying direction of the circulating conveying mechanism 3; the sample storage mechanism 2 is installed on the lower platform 12; the sample loading mechanism 6 is used to transfer the sample of the sample storage mechanism 2 to the sedimentation bin on the circulating conveying mechanism 3.
[0042] The glass slide loading mechanism 4, the sample loading mechanism 6, the coding mechanism, the liquid adding mechanism 8 and the sealing mechanism 10 are all conventional existing technologies and will not be described in detail here; the glass slide loading mechanism 4 loads the glass slide to the circulating conveying mechanism 3, and the circulating conveying mechanism 3 transports the glass slide through the sedimentation bin loading mechanism 5 for sedimentation bin loading. After the sample loading mechanism 6 takes the sample from the sample storage mechanism 2 and the coding mechanism codes it, the liquid adding mechanism 8 performs staining and slide preparation, and then the sealing mechanism 10 performs sealing in time.
[0043] The processes corresponding to the liquid adding mechanism 8 and the film sealing mechanism 10 are connected and arranged to ensure wet sealing of the film and avoid dry sealing, thereby ensuring the film production quality.
[0044] The lower platform 12 is provided with a transfer rail frame 121 , on the track of which a plurality of fixing clips 122 are slidably provided, and the bottom of the sample storage mechanism 2 is mounted on the fixing clips 122 ; the transfer rail frame 121 can carry at least two sets of sample storage mechanisms 2 at the same time.
[0045] The sample storage mechanism 2 can be moved on the transfer rail frame 121 after being fixed by the fixing clamp 122. For example, if two groups of sample storage mechanisms 2 are carried at the same time, after one group of sample storage mechanisms 2 has taken out all the samples, it can be moved to the side for removal, which is conducive to rapid sample supply.
[0046] The sample storage mechanism 2 comprises a syringe storage area and a sample storage area. The syringe storage area stores a sampling syringe 21 , and the sample storage area stores a sample bottle 22 . The sampling syringes 21 correspond to the sample bottles 22 one by one.
[0047] The sampling syringe 21 corresponds to the sample bottle 22 one by one, and the sampling syringe 21 is disposable, ensuring that the sample bottle 22 will not be contaminated at all.
[0048] The loading mechanism 6 is provided with a mechanical clamp, which can take a sample from the sample bottle 22 with the sampling syringe 21 and then add the sample in the sampling syringe 21 into the sedimentation bin of the loading station of the circulation conveying mechanism 3 .
[0049] The mechanical gripper grabs the sampling syringe 21 and moves it to the top of the sample bottle 22 to extract the sample, and then moves it to the top of the sedimentation bin of the sample loading station of the circulation conveying mechanism 3 to inject the sample.
[0050] The sampling syringe 21 includes a sampling barrel 211, a needle tube 212 and a piston push rod 213, wherein: the needle tube 212 is installed at the front end of the sampling barrel 211; the needle tube 212 is connected to the interior of the sampling barrel 211; the piston end of the piston push rod 213 is inserted into the rear end of the sampling barrel 211; the piston end of the piston push rod 213 can perform piston movement inside the sampling barrel 211; a ring platform is provided on the circumference of the sampling barrel 211; the outer diameter of the ring platform is larger than the aperture size of the syringe jack; the needle tube 212 is used to be inserted into the sample bottle 22 to connect the sample bottle 22 with the interior of the sampling barrel 211; the piston push rod 213 is used to create negative pressure inside the sampling barrel 211.
[0051] The sample storage area is provided with a first frame plate 23 and a second frame plate 24. The first frame plate 23 is arranged parallel to the second frame plate 24. The first frame plate 23 is provided with a placement hole, and the second frame plate 24 is provided with a lifting hole corresponding to the placement hole one by one; the aperture of the placement hole is larger than the diameter of the sample bottle 22, and the aperture of the lifting hole is smaller than the diameter of the sample bottle 22. The sample bottle 22 is inserted into the placement hole and placed on the lifting hole; the sample storage mechanism 2 also includes a lifting component 27, a rotating component 25 and a code scanning component 26; the rotating component 25 is installed on the lifting end of the lifting component 27; the rotating part of the rotating component 25 is arranged below the lifting hole; the rotating part of the rotating component 25 can pass through the lifting hole to contact the bottom of the sample bottle 22; the scanning end of the code scanning component 26 corresponds to the side of the sample bottle 22.
[0052] The sample bottle 22 is placed on the second rack 24. The placement hole is used to limit the sample bottle 22 to prevent the sample bottle 22 from tipping over. The lifting hole at the bottom of the sample bottle 22 is used to pass through the rotating component 25. The rotating component 25 lifts the sample bottle 22 away from the second rack 24 to eliminate the friction between the sample bottle 22 and the second rack 24, so that the rotating component 25 can rotate to drive the sample bottle 22. The sample bottle 22 continues to rotate until the information label on the side is aligned with the scanning end of the scanning component 26.
[0053] The sample bottle 22 is rotated by the rotating component 25 until the code is scanned successfully. There is no need to place the sample bottle 22 in a fixed posture, which facilitates the rapid placement of the sample bottle 22.
[0054] The sedimentation bin feeding mechanism 5 includes a feeding clamp 51, a temporary storage structure 52, a material transfer clamp 53 and a sorting structure 54, wherein: the feeding clamp 51 is arranged at the rear storage passage of the temporary storage structure 52; the material transfer clamp 53 is arranged at the side of the temporary storage structure 52; the sorting structure 54 is arranged at the front storage passage of the temporary storage structure 52; the feeding clamp 51 is used to transfer the sedimentation bin of the temporary storage structure 52 to the circulating conveying mechanism 3; the material transfer clamp 53 is used to transfer the sedimentation bin sorted by the sorting structure 54 to the temporary storage structure 52; the sorting structure 54 is used to organize the scattered sedimentation bins into sedimentation bins with fixed postures.
[0055] The sorting structure 54 sends the sedimentation bin out in a fixed posture, and the material transfer clamp 53 transfers it to the temporary storage structure 52. When the sedimentation bin needs to be loaded, the loading clamp 51 takes the sedimentation bin out of the temporary storage structure 52 for loading.
[0056] By setting the sorting structure 54, the sedimentation bin can be automatically sorted without manual adjustment of the sedimentation bin posture, and can run automatically for a long time.
[0057] The sorting structure 54 includes a material accumulation box 541 and a push rod 542, wherein: the material accumulation box 541 is a funnel structure; the top end of the push rod 542 passes through the bottom of the material accumulation box 541; the top end of the push rod 542 corresponds to the inner hole of the sedimentation bin; the bottom end of the push rod 542 is provided with a driving device; the driving device is used to drive the push rod 542 to be pushed upward from the bottom of the material accumulation box 541.
[0058] The push rod 542 pushes upward into the material accumulation box 541, fits into a sedimentation bin inside the material accumulation box 541, and pushes the sedimentation bin out of the material accumulation box 541 to be taken away by the material transfer clamp 53.
[0059] The circulating conveying mechanism 3 adopts a circulating conveying setting.
[0060] A control method for a fully automatic liquid-based cell slide dyeing and sealing system comprises the following steps:
[0061] Step 1: The lifting component 27 lifts the rotating component 25, and the rotating component 25 lifts the sample bottle 22 to the scanning area of the code scanning component 26. The rotating component 25 adjusts the direction of the information label on the side of the sample bottle 22 by rotating, so that the code scanning component 26 obtains the sample information;
[0062] Step 2: the glass slide loading mechanism 4 loads the corresponding glass slide onto the circulation conveying mechanism 3 according to the sample information;
[0063] Step 3, when the glass slide is transported to the station where the sedimentation bin loading mechanism 5 is located, the sedimentation bin loading mechanism 5 loads the sedimentation bin onto the glass slide;
[0064] Step 4: When the glass slide and the sedimentation chamber are transported to the station where the sample loading mechanism 6 is located, the mechanical gripper clamps the sampling syringe 21 to extract the sample in the corresponding sample bottle 22, and then adds the extracted sample into the sedimentation chamber;
[0065] Step 5, when the glass slide and the sedimentation bin are transported to the station where the coding mechanism is located, the coding mechanism codes the sedimentation bin;
[0066] Step six, after the glass slide and the sample in the sedimentation chamber have been settled, they are transported to the workstation where the liquid adding mechanism 8 is located. The liquid adding mechanism 8 first fixes the sedimentation chamber with the cleaning liquid, then cleans it with the buffer solution, and adds the hematoxylin dye solution. After the first step of staining is completed, the buffer solution is used for cleaning, and the EA dye solution is added. After the second step of staining is completed, the cleaning liquid is added for cleaning, and then the transparent liquid is added to complete the staining and preparation.
[0067] Step 7, when the glass slide and the sedimentation bin are transported to the station where the sedimentation bin unloading mechanism 9 is located, the sedimentation bin unloading mechanism 9 takes away the sedimentation bin and discards it;
[0068] Step eight, when the glass slide is transported to the workstation where the sealing mechanism 10 is located, the sealing mechanism 10 takes the glass slide, adds sealing glue, adsorbs the cover glass, seals the glass slide, and then puts the sealed glass slide into the glass slide collection box.
[0069] Those skilled in the art will readily appreciate other embodiments of the present invention after considering the specification and practicing the invention invented herein. This application is intended to cover any variations, uses or adaptations of the present invention that follow the general principles of the present invention and include common knowledge or customary techniques in the art that are not invented by the present invention. The specification and examples are intended to be exemplary only, and the true scope and spirit of the present invention are indicated by the appended claims.
[0070] It should be understood that the present invention is not limited to the exact construction that has been described above and shown in the drawings and that various modifications and changes may be made without departing from the scope thereof. The scope of the present invention is limited only by the appended claims.
Claims
1. A fully automatic liquid-based cell slide preparation and staining system, characterized in that: The fully automatic liquid-based cell slide preparation, staining and sealing system comprises a loading platform, a sample storage mechanism, a lowering platform, a circulating conveying mechanism, a slide loading mechanism, a sedimentation bin loading mechanism, a sample loading mechanism, a coding mechanism, a liquid adding mechanism, a sedimentation bin unloading mechanism, a sealing mechanism and a slide collecting mechanism, wherein: The upper platform is mounted above the lower platform; The circulating conveying mechanism, the glass slide loading mechanism, the sedimentation bin loading mechanism, the sample loading mechanism, the coding mechanism, the liquid adding mechanism, the sedimentation bin unloading mechanism, the sealing mechanism and the collecting mechanism are all installed on the upper platform; The glass slide loading mechanism, the sedimentation bin loading mechanism, the sample loading mechanism, the coding mechanism, the liquid adding mechanism, the sedimentation bin unloading mechanism, the sealing mechanism and the collecting mechanism are sequentially arranged on the side of the circulating conveying mechanism along the conveying direction of the circulating conveying mechanism; The sample storage mechanism is installed on the lower stand; The sample loading mechanism is used to transfer the sample from the sample storage mechanism to the sedimentation bin on the circulating conveying mechanism; The sample storage mechanism comprises a syringe storage area and a sample storage area, wherein the syringe storage area stores sampling syringes, and the sample storage area stores sample bottles, and the sampling syringes correspond to the sample bottles one by one; The sample loading mechanism is provided with a mechanical clamp, which can take the sample from the sample bottle with the sampling syringe and then add the sample in the sampling syringe into the sedimentation bin of the sample loading station of the circulation conveying mechanism; The sampling syringe comprises a sampling cylinder, a needle tube and a piston push rod, wherein: The needle tube is installed at the front end of the sampling cylinder; The needle tube is connected to the interior of the sampling cylinder; The piston end of the piston push rod is inserted into the rear end of the sampling cylinder; The piston end of the piston push rod can perform piston motion inside the sampling cylinder; A ring platform is provided around the sampling tube; The needle tube is used to be inserted into the sample bottle to connect the sample bottle with the interior of the sampling cylinder; The piston push rod is used to create negative pressure inside the sampling cylinder; The sample storage area is provided with a first frame plate and a second frame plate, the first frame plate is arranged parallel to the upper side of the second frame plate, the first frame plate is provided with a placement hole, and the second frame plate is provided with a lifting hole corresponding to the placement hole one by one; the aperture of the placement hole is larger than the diameter of the sample bottle, and the aperture of the lifting hole is smaller than the diameter of the sample bottle, and the sample bottle is inserted into the placement hole and placed on the lifting hole; The sample storage mechanism further comprises a lifting assembly, a rotating assembly and a code scanning assembly; the rotating assembly is mounted on the lifting end of the lifting assembly; the rotating part of the rotating assembly is arranged below the lifting hole; the rotating part of the rotating assembly can pass through the lifting hole to contact the bottom of the sample bottle; the code scanning end of the code scanning assembly corresponds to the side of the sample bottle; The sedimentation bin feeding mechanism includes a feeding clamp, a temporary storage structure, a material transfer clamp and a sorting structure, wherein: The feeding clamp is arranged at the material storage rear channel of the temporary storage structure; The material transfer clamp is arranged on the side of the temporary storage structure; The sorting structure is arranged at the front passage of the temporary storage structure; The feeding clamp is used to transfer the sedimentation bin of the temporary storage structure to the circulating conveying mechanism; The material transfer clamp is used to transfer the sedimentation bin sorted by the sorting structure to the temporary storage structure; The sorting structure is used to organize the scattered sedimentation bins into sedimentation bins with fixed postures; The sorting structure includes a material accumulation box and a top rod, wherein: The material accumulation box is in a funnel structure; The top end of the push rod passes through the bottom of the material storage box; The top end of the push rod corresponds to the inner hole of the sedimentation bin; The bottom end of the top rod is provided with a driving device; The driving device is used to drive the ejector rod to eject upward from the bottom of the material accumulation box.
2. The fully automatic liquid-based cell slide preparation and staining system according to claim 1, characterized in that: The lower platform is provided with a transfer rail frame, and a plurality of fixing clips are slidably provided on the track of the transfer rail frame, and the bottom of the sample storage mechanism is installed on the fixing clips; The transfer rail frame can carry at least two sets of the sample storage mechanisms simultaneously.
3. The fully automatic liquid-based cell slide preparation and staining system according to claim 1, characterized in that: The circulating conveying mechanism adopts an integrated circulating conveying structure.
4. A control method for a fully automatic liquid-based cell slide staining and sealing system, based on the fully automatic liquid-based cell slide staining and sealing system according to any one of claims 1 to 3, characterized in that: The following steps are involved: Step 1: The lifting component lifts the rotating component, and the rotating component lifts the sample bottle to the scanning area of the code scanning component. The rotating component adjusts the direction of the information label on the side of the sample bottle by rotation, so that the code scanning component obtains the sample information; Step 2: the glass slide loading mechanism loads the corresponding glass slide onto the circulating conveying mechanism according to the sample information; Step 3, when the glass slide is transported to the station where the sedimentation bin loading mechanism is located, the sedimentation bin loading mechanism loads the sedimentation bin onto the glass slide; Step 4, when the glass slide and the sedimentation chamber are transported to the station where the sample loading mechanism is located, the mechanical gripper clamps the sampling syringe to extract the sample in the corresponding sample bottle, and then adds the extracted sample into the sedimentation chamber; Step 5, when the glass slide and the sedimentation bin are transported to the station where the coding mechanism is located, the coding mechanism codes the sedimentation bin; Step 6, after the glass slide and the sample in the sedimentation chamber are precipitated, they are transported to the station where the liquid adding mechanism is located, and the liquid adding mechanism first fixes the sedimentation chamber with a cleaning solution, then washes with a buffer solution, and adds a hematoxylin dye solution. After the first step of dyeing is completed, the buffer solution is washed, and the EA dye solution is added. After the second step of dyeing is completed, the cleaning solution is added for washing, and then a transparent liquid is added to complete the dyeing and slide preparation; Step 7, when the glass slide and the sedimentation bin are transported to the station where the sedimentation bin unloading mechanism is located, the sedimentation bin unloading mechanism takes away the sedimentation bin and discards it; Step eight, when the glass slide is transported to the workstation where the sealing mechanism is located, the sealing mechanism takes away the glass slide, adds sealing glue, adsorbs the cover glass, seals the glass slide, and then puts the sealed glass slide into a glass slide collection box.
Citation Information
Patent Citations
Long-stroke automatic feeding device
CN111232629A
Cell slide preparation device
CN112179739A
Bar code identification device for placing samples in multiple circles
CN212932305U
Medical testing machine
CN216559968U
