A portable rapid detection device for Microcystis aeruginosa

Abstract

The present invention relates to a portable rapid detection device for Microcystis aeruginosa, which comprises a detection device box body, wherein the inner space of the detection device box body is divided into a signal processing area and a signal receiving area by a fixing plate inside the detection device box body; a signal processing module is arranged in the signal processing area; an absorption cell for placing a cuvette is arranged in the signal receiving area, a photoelectric sensor placement cell for placing a photoelectric sensor is arranged on the side of the absorption cell close to the fixing plate, and a light-through hole is opened between the absorption cell and the photoelectric sensor placement cell; a fixing hole is opened on the side of the absorption cell away from the fixing plate, a rubber fixing sheet is arranged in the fixing hole, and a light-emitting diode is arranged on the inner end surface of the rubber fixing sheet; the excitation light emitted by the light-emitting diode is received by the photoelectric sensor through the fixing hole, the absorption cell and the light-through hole; the photoelectric sensor is connected to the signal processing module. The detection device of the present invention has a simple structure, is easy to carry, and can quickly detect the concentration of Microcystis aeruginosa in a solution.

Description

Technical Field

[0001] The invention relates to the detection of Microcystis aeruginosa, in particular to a portable rapid detection device of Microcystis aeruginosa, belonging to the technical field of algae detection equipment. Background Art

[0002] In recent years, human activities have caused a sharp deterioration of the water environment, and a series of water ecological problems caused by eutrophication have become increasingly serious, attracting widespread attention from the world. Eutrophication of water bodies refers to the phenomenon that a large amount of nutrients such as nitrogen and phosphorus required for biological growth flow into slow-flowing water bodies, causing water pollution. Its most prominent feature is that algae and other plankton reproduce rapidly in a short period of time, consume the amount of dissolved oxygen in the water, and cause the death of aquatic plants and animals. At the same time, due to eutrophication of water bodies, blue algae and green algae are dominant species of algae that grow in large quantities on the surface of the water body, forming a layer of "green scum", which reduces the transparency of the water. The underwater algae die because they cannot photosynthesize, not only emitting a foul odor, but also releasing various types of algae toxins into the water body after the algae cells rupture, which will also threaten the life and health of other organisms. Among them, microcystins have the highest frequency, the largest production and the most serious harm in algal bloom pollution. Therefore, it is necessary to quickly obtain the concentration information of microcystins in water to achieve pollution warning and ensure the safety of drinking water. At present, the method of measuring algae concentration is mainly to collect water samples on site and send them to the laboratory, which is not only time-consuming and laborious, but also will miss the best time to warn of algae outbreaks. Therefore, there is an urgent need for a simple, efficient and economical device to achieve on-site rapid detection of Microcystis concentration. Summary of the invention

[0003] The present invention aims to overcome the above-mentioned deficiencies of the prior art and provide a portable rapid detection device for Microcystis aeruginosa. The detection device has a simple structure, is easy to carry, and can quickly detect the concentration of Microcystis aeruginosa in a solution.

[0004] The technical solution adopted to achieve the purpose of the present invention is a portable rapid detection device for Microcystis aeruginosa, which includes a detection device box body, wherein the internal space of the detection device box body is divided into a signal processing area and a signal receiving area by a fixing plate inside the detection device box body; a signal processing module is arranged in the signal processing area; an absorption cell for placing a cuvette is arranged in the signal receiving area, a photoelectric sensor placement cell for placing a photoelectric sensor is arranged on the side of the absorption cell close to the fixing plate, and a light-through hole is opened between the absorption cell and the photoelectric sensor placement cell; a fixing hole is opened on the side of the absorption cell away from the fixing plate, a rubber fixing sheet is arranged in the fixing hole, and a light-emitting diode is arranged on the inner end surface of the rubber fixing sheet; the light-through hole is on the same horizontal line as the center of the fixing hole, and the excitation light emitted by the light-emitting diode is received by the photoelectric sensor through the fixing hole, the absorption cell and the light-through hole; the photoelectric sensor is connected to the signal processing module; and a cuvette placement rack for placing a cuvette is also arranged in the signal receiving area.

[0005] In the above technical solution, a pin is connected to one end of the photoelectric sensor, and a slot is provided on the side of the photoelectric sensor placement pool close to the fixed plate. The pin of the photoelectric sensor is placed in the photoelectric sensor placement pool through the slot, and the pin is connected to the signal processor through an electric wire to transmit the electrical signal to the signal processing module.

[0006] In the above technical solution, the signal processing module includes a current amplifier, a signal converter, a development board and an expansion board. The current amplifier and the signal converter are placed horizontally, the current amplifier is located below the signal converter, and the current amplifier and the signal converter are connected through a built-in card slot; the development board and the expansion board are placed vertically; the current amplifier is used to amplify the weak current generated by the photoelectric sensor, the signal converter converts the electrical signal into a digital signal, and the development board and the expansion board are used to import a program with a known Microcystis aeruginosa concentration and an electrical signal.

[0007] Furthermore, a limit block is provided at the bottom of the signal processing area of ​​the portable Microcystis aeruginosa rapid detection device, and a vertical limit plate is connected to the limit block, and the vertical limit plate is used to fix the current amplifier, the signal converter, the development board and the expansion board.

[0008] Furthermore, the signal processing area of ​​the portable Microcystis aeruginosa rapid detection device is provided with a power block for supplying power to the light-emitting diode, the current amplifier, the signal converter, the development board and the expansion board.

[0009] Furthermore, a box cover is provided above the signal receiving area of ​​the portable Microcystis aeruginosa rapid detection device. The box cover is connected to the detection device box body through a hinge on one side close to the cuvette placement rack, and is connected to the lock on the detection device box body through a card block on the other side.

[0010] In the above technical solution, a power switch button, a liquid crystal display and an indicator light are embedded on the left side of the upper surface of the detection device box body. The power switch button is connected to the power block, and the liquid crystal display and the indicator light are connected to the signal processing module.

[0011] The portable rapid detection device for Microcystis aeruginosa of the present invention only needs to put the solution to be detected into a cuvette, cover the cuvette cover, put the cuvette into an absorption cell, cover the box cover, and press the power switch button to automatically detect the concentration of the Microcystis aeruginosa solution, and can be intuitively displayed through the liquid crystal display screen on the surface of the detection device box body. The detection process is automatically completed by the components inside the signal receiving area and the signal processing area, and no additional manual operation is required. Therefore, the detection device is easy to use and can quickly detect the concentration of Microcystis aeruginosa in the solution to be tested. The detection device of the present invention has a simple structure, is easy to carry, is conducive to rapid detection in different occasions, thereby improving the efficiency of detection, and in addition, the cost of each component used in the device of the present invention is economical. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the structure of the portable Microcystis aeruginosa rapid detection device of the present invention.

[0013] Figure 2 This is a schematic diagram of the internal structure of the portable Microcystis aeruginosa rapid detection device of the present invention after the lid is opened.

[0014] Figure 3 This is a front schematic diagram of the portable Microcystis aeruginosa rapid detection device of the present invention after the lid is opened.

[0015] Figure 4 This is a schematic diagram of the back side of the portable Microcystis aeruginosa rapid detection device of the present invention after the lid is opened.

[0016] In the figure, 1-power switch button, 2-LCD display, 3-indicator light, 4-box cover, 5-card block, 6-hole of cuvette holder, 7-cuvette holder, 8-lock, 9-vertical limit plate, 10-limit block, 11-power block, 12-fixed plate, 13-fixed block, 14-absorption cell, 15-photoelectric sensor holder, 16-photoelectric sensor, 17-light-emitting diode pin, 18-card slot, 19-hinge, 20-current amplifier, 21-signal converter, 22-development board, 23-expansion board, 24-signal processing area, 25-signal receiving area, 26-light-emitting diode, 27-rubber fixing sheet, 28-light-through hole. DETAILED DESCRIPTION

[0017] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0018] like Figure 1-2As shown, the portable rapid detection device for Microcystis aeruginosa of the present invention comprises a detection device box body, and the internal space of the detection device box body is divided into a signal processing area 24 and a signal receiving area 25 by a fixing plate 12, wherein:

[0019] The signal receiving area 25 is provided with an absorption pool 14 for placing a cuvette, and a photoelectric sensor placement pool 15 for placing a photoelectric sensor 16 is provided on the side of the absorption pool 14 close to the fixed plate 12 (the left side in the figure), and a light-through hole 28 is opened between the absorption pool 14 and the photoelectric sensor placement pool 15; a circular fixing hole is opened on the side of the absorption pool 14 away from the fixed plate 12 (the right side in the figure), and a rubber fixing sheet 27 is arranged in the circular fixing hole, and a light-emitting diode 26 is arranged on the inner end surface of the rubber fixing sheet 27; the light-through hole 28 is on the same horizontal line as the center of the circular fixing hole, and the excitation light emitted by the light-emitting diode 26 passes through the fixing hole, the absorption pool 14, and the light-through hole 28 and is received by the photoelectric sensor 16; the signal receiving area is also provided with a cuvette placement rack 7 for placing a cuvette. In this embodiment, a cuvette placement rack hole 6 is opened on the upper surface of the cuvette placement rack 7, and the cuvette is placed in the cuvette placement rack hole 6. One end of the photoelectric sensor 16 is connected with a pin, and a slot is provided on the side of the photoelectric sensor placement pool 15 close to the fixing plate 12 (the left side in the figure), and the pin of the photoelectric sensor 16 is placed in the photoelectric sensor placement pool 15 through the slot.

[0020] A signal processing module is provided in the signal processing area 24, and the pins of the photoelectric sensor 16 are connected to the signal processing module through wires to transmit the electrical signal to the signal processing module. Specifically, the signal processing module of the present invention includes a current amplifier 20, a signal converter 21, a development board 22 and an expansion board 23. The current amplifier 20 and the signal converter 21 are placed horizontally, and the current amplifier 20 is located below the signal converter 21. The current amplifier 20 and the signal converter 21 are connected through a self-contained card slot; the development board 22 and the expansion board 23 are placed vertically. In order to keep the signal processing module fixed and stable, the present embodiment can also be provided with a limit block 10 at the bottom of the signal processing area 21. The limit block 10 can be set to four blocks, and the four limit blocks 10 can be respectively located at the positions of the four corners. A vertical limit plate 9 is connected to the limit block 10, and the vertical limit plate 9 is used to fix the current amplifier 20, the signal converter 21, the development board 22 and the expansion board 23. The current amplifier 20 is used to amplify the weak current generated by the photoelectric sensor 16, the signal converter 21 converts the electrical signal into a digital signal, and the development board 22 and the expansion board 23 are used to import a program with known Microcystis aeruginosa concentration and electrical signal.

[0021] A power block 11 for supplying power to the light emitting diode 26 , the current amplifier 20 , the signal converter 21 , the development board 22 and the expansion board 23 is also provided at the bottom of the signal processing area 24 .

[0022] In order to facilitate fixing of the components in the signal receiving area 25 and improve the portability of the present invention, a box cover 4 is provided above the signal receiving area 25 of the present invention. Figure 3 and Figure 4 As shown, the box cover 4 is connected to the detection device box body through a hinge 19 on one side close to the cuvette placement rack 7, and is connected to the lock buckle 8 on the detection device box body through a clamping block 5 on the other side.

[0023] In order to facilitate operation and display, the present invention has a power switch button 1, a liquid crystal display screen 2 and an indicator light 3 embedded on the upper surface of the detection device box above the signal processing area 24. The power switch button 1 is connected to the power block 11, and the liquid crystal display screen 2 and the indicator light 3 are connected to the signal processing module. The development board 22 and the expansion board 23 obtain the digital signal transmitted by the signal converter 21 and process it. The measured value obtained by the processing is transmitted to the liquid crystal display screen 2 for display.

[0024] The use process and working principle of the portable Microcystis aeruginosa rapid detection device of the present invention are as follows:

[0025] When in use, remove the lock buckle 8 on the box cover 4 from the right block 5 at the top of the outer side of the box body, open the box cover, take out the cuvette on the cuvette placement rack 7, put the solution to be tested into the cuvette, cover the cuvette cover, put the cuvette into the absorption pool 14, press the power switch button 1, the light emitting diode 26, the indicator light 3, and the liquid crystal display 2 light up, indicating that the detection device is started normally, cover the box cover 4, and to ensure safety during the detection process, the lock buckle 8 on the box cover 4 can be put on the block 5.

[0026] The light emitting diode 26 emits 590nm visible light. When the stock solution of Microcystis aeruginosa placed in the cuvette in the absorption cell 14 is irradiated with 590nm visible light, its unique phycocyanin will immediately release the absorbed energy in the form of radiation transition after being stimulated by strong light, and emit a fluorescence wavelength centered at 630nm. The 630nm fluorescence is received by the photoelectric sensor 16 after passing through the light hole 28. The light signal received by the photoelectric sensor 16 is processed by the current amplifier 20 and the signal converter 21 to obtain a digital signal of fluorescence intensity. Since when the solution has a certain concentration, according to the Lambert-Beer law, when the excitation light intensity and wavelength are constant, the fluorescence intensity emitted by the fluorescent substance is proportional to the concentration of the cyanobacteria solution. Therefore, the digital signal of fluorescence intensity is converted to obtain a digital signal of the concentration of the Microcystis aeruginosa solution and then transmitted to the liquid crystal display 2 for display.

[0027] After the detection is completed, read the data on the LCD screen 2, press the power switch button 1 to turn off the detection device, take out the cuvette, clean the liquid in the cuvette, put it back into the cuvette placement rack 7, and then put the lock 8 on the box cover 4 onto the card block 5.

Claims

1. A portable rapid detection device for Microcystis aeruginosa, characterized in that: The invention comprises a detection device box body, wherein the interior space of the detection device box body is divided into a signal processing area and a signal receiving area by a fixing plate (12); a signal processing module is arranged in the signal processing area; an absorption pool (14) for placing a cuvette is arranged in the signal receiving area, a photoelectric sensor placement pool (15) for placing a photoelectric sensor (16) is arranged on a side of the absorption pool (14) close to the fixing plate (12), and a light-through hole (28) is provided between the absorption pool (14) and the photoelectric sensor placement pool (15); a fixing hole is arranged on a side of the absorption pool (14) away from the fixing plate (12), a rubber fixing sheet (27) is arranged in the fixing hole, and a light-emitting diode (26) is arranged on the inner end surface of the rubber fixing sheet (27); the light-through hole (28) and the center of the fixing hole are on the same horizontal line, and the excitation light emitted by the light-emitting diode (26) is received by the photoelectric sensor (16) through the fixing hole, the absorption pool and the light-through hole; the photoelectric sensor (16) is connected to the signal processing module; and a cuvette placement rack for placing the cuvette is also arranged in the signal receiving area; One end of the photoelectric sensor (16) is connected to a pin, and a slot is provided on a side of the photoelectric sensor placement pool (15) close to the fixing plate (12). The pin of the photoelectric sensor (16) is placed in the photoelectric sensor placement pool (15) through the slot, and the pin is connected to the signal processor through an electric wire to transmit the electrical signal to the signal processing module; The signal processing module comprises a current amplifier (20), a signal converter (21), a development board (22) and an expansion board (23); the current amplifier (20) and the signal converter (21) are placed horizontally, the current amplifier (20) is located below the signal converter (21), and the current amplifier (20) and the signal converter (21) are connected via a self-contained card slot; the development board (22) and the expansion board (23) are placed vertically; the current amplifier (20) is used to amplify the weak current generated by the photoelectric sensor (16), the signal converter (21) converts the electrical signal into a digital signal, and the development board (22) and the expansion board (23) are used to import a program with a known concentration of Microcystis aeruginosa and an electrical signal; A limit block (10) is provided at the bottom of the signal processing area, and a vertical limit plate (9) is connected to the limit block (10), and the vertical limit plate (9) is used to fix the current amplifier (20), the signal converter (21), the development board (22) and the expansion board (23); The signal processing area is provided with a power supply block (11) for supplying power to the light-emitting diode (26), the current amplifier (20), the signal converter (21), the development board (22) and the expansion board (23); A box cover is provided above the signal receiving area, one side of the box cover close to the cuvette placement rack is connected to the detection device box body through a hinge, and the other side of the box cover is connected to the lock on the detection device box body through a card block; A power switch button (1), a liquid crystal display screen (2) and an indicator light (3) are embedded on the left side of the upper surface of the detection device box body; the power switch button (1) is connected to a power block (11), and the liquid crystal display screen (2) and the indicator light (3) are connected to a signal processing module; finally, a digital signal of fluorescence intensity is obtained, which is converted and processed to obtain a digital signal of the concentration of the Microcystis aeruginosa solution, and then transmitted to the liquid crystal display screen (2) for display.

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