A method for preparing a stropharia substitute culture medium and a mushroom bag

Abstract

The invention discloses a spherical cap mushroom substitute culture medium and a method for preparing a mushroom bag. The formula of the substitute culture medium and the weight ratio of each component are: licorice residue, corn flour, glucose, potassium dihydrogen phosphate, magnesium sulfate, calcium hydroxide, etc. And combined with a specific mushroom bag preparation method: according to the culture medium formula, each raw material is mixed evenly, and water is added and stirred to fully mix, the material-water ratio is 1:1.5-2.4, and the culture material is filled to no more than 2 / 3 of the volume of the mushroom bag during bagging, and the culture material is compacted, and the compactness is moderate, and then the ring and the cover are buckled, and a large space is left in the upper part of the mushroom bag, and the material bag is sterilized with high pressure steam at 121°C for 2.5-3 hours. The substitute culture medium and the method for preparing the mushroom bag of the present invention can provide suitable nutritional conditions and a breathable space environment for the growth of spherical cap mushroom mycelium, can significantly improve the growth rate, density and growth of spherical cap mushroom mycelium, improve the quality of the strain, and provide favorable technical support for the safe and efficient seed production of spherical cap mushroom.

Description

Technical Field

[0001] The invention relates to the technical field of edible fungus strain breeding, specifically to the technical field related to the breeding of Stropharia capillaris strains, and more specifically to the technical field of a Stropharia capillaris strain substitute culture medium and a mushroom bag preparation method. Background Art

[0002] Stropharia rugosoannulata, also known as wine-red Stropharia rugosoannulata, Pei's Stropharia rugosoannulata, and red pine mushroom, is a rising star in edible fungi. Stropharia rugosoannulata has tender meat, crisp stem, delicious taste, fresh fragrance, rich aroma of dried mushrooms, and rich nutrition. The fruiting body contains rich protein, polysaccharides, vitamins and minerals, and a full range of amino acids. It enjoys the reputation of "mountain treasures". Stropharia rugosoannulata polysaccharides have the effects of aiding digestion, preventing coronary heart disease, and relieving mental fatigue. It also has the effects of lowering blood pressure and cholesterol, improving human immunity, and anti-tumor activity.

[0003] However, the production of stropharia is a major problem in the development of the industry. Preliminary investigations and studies have found that the cultivation time of stropharia original and cultivated species is generally more than 60 days, and the fastest is 40 days. At the same time, the mycelium is often weak due to inappropriate culture medium formula and unreasonable preparation method, and the mycelium cannot grow full of fungus bags. Even if the relevant substitute culture medium and preparation method of the prior art are used for reference, it is still impossible to obtain a good cultivation effect. "Stropharia" is a species of edible fungi different from "stropharia, enoki mushroom, enoki mushroom, enoki mushroom, enoki mushroom, enoki mushroom, enoki mushroom", and its living habits, cultivation methods, and mycelium growth requirements for nutrition and environmental conditions are all different. Compared with other species, the mycelium of stropharia has a greater demand for oxygen and is more sensitive to an oxygen-deficient environment. When cultivating mycelium, if the ventilation is slightly insufficient, the mycelium will grow slowly or even stagnate, and the mycelium is weak. In addition, compared with other species, when using containers (such as fungus bags or fungus bottles) to cultivate Stropharia cap mushroom mycelium, Stropharia cap mushroom has more stringent requirements on the culture medium formula. Currently, the raw materials and formulas suitable for its growth are very limited, and the developed culture medium formulas are not very ideal, mainly because the culture time is too long.

[0004] Therefore, there is an urgent need to develop new raw materials and formulas for the substitute culture medium for Pleurotus ostreatus and to explore a method for preparing fungus bags that can enhance the growth vigor and growth rate of mycelium, in order to provide a guarantee for the safe and efficient seed production of Pleurotus ostreatus. Summary of the invention

[0005] In view of the technical status that the formula of the substitute culture medium in the prior art during the cultivation of Stropharia is still not suitable for the growth of Stropharia mycelium, and cannot meet the nutritional requirements of the good and fast growth of the mycelium; and the preparation method of the Stropharia mushroom bag is still unreasonable, especially the air permeability in the mushroom bag is poor, and the oxygen demand of the mycelium growth cannot be met, this application aims to provide a Stropharia mushroom substitute culture medium and a method for preparing a mushroom bag, a substitute culture medium formula and the weight of each component, and a specific method for preparing a mushroom bag. The substitute culture medium and the method for preparing a mushroom bag of the present invention can provide suitable nutritional conditions and a breathable space environment for the growth of Stropharia mushroom mycelium, can significantly improve the growth rate, density and growth potential of Stropharia mushroom mycelium, improve the quality of the strain, and provide favorable technical support for the safe and efficient production of Stropharia mushroom seeds.

[0006] The present invention is achieved through the following technical solutions:

[0007] The present application provides a stropharia substitute culture medium, wherein the formula of the substitute culture medium comprises, by weight percentage: 10%-94.6% of licorice residue, 0%-84.6% of corn cobs, 2% of corn flour, 2% of glucose, 0.3% of potassium dihydrogen phosphate, 0.1% of magnesium sulfate, and 1% of calcium hydroxide.

[0008] Preferably, the formula of the feed substitute culture medium contains, by weight percentage: 30%-94.6% licorice residue, 0%-64.6% corn cob, 2% corn flour, 2% glucose, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and 1% calcium hydroxide.

[0009] More preferably, the formula of the feed substitute culture medium contains, by weight percentage: 94.6% liquorice residue, 2% glucose, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and 1% calcium hydroxide.

[0010] Furthermore, the present application also provides a method for preparing a Stropharia cap mushroom bag, wherein the Stropharia cap mushroom bag is prepared by the following steps:

[0011] (1) pre-wetting fresh, non-moldy licorice residue and corn cobs with tap water for more than 12 hours to completely saturate them, and then evenly mixing corn flour, glucose, potassium dihydrogen phosphate, magnesium sulfate, and calcium hydroxide according to the formula ratio;

[0012] (2) adjusting the water content of the culture medium to 60-70% with tap water, testing the pH of the culture medium, and making sure that the pH value is between 9 and 11. After the culture medium is prepared, bag it in time and do not store it overnight;

[0013] (3) The culture medium in step (2) is packaged, and a high temperature and high pressure resistant angled bag with a specification of 13-18 cm × 23-38 cm × 0.0025-0.004 cm is selected. When bagging, the culture medium is loaded into the bag, compacted to a moderate degree of compaction, and then the ring is put on and the cover is buckled, leaving a large space in the upper half of the bag. The loaded bag is sterilized by high pressure steam at 121° C. for 2.5-3 hours. After the sterilization is completed, inoculate in time when the bag temperature drops below 30° C.

[0014] Preferably, the mixed components use tap water to adjust the moisture content of the culture medium so that the material-water ratio is 1:1.5-2.4.

[0015] More preferably, the mixed components use tap water to adjust the moisture content of the culture medium so that the material-water ratio is 1:2.2.

[0016] Preferably, when bagging, the culture medium is loaded into the bag so as not to exceed 2 / 3 of the volume of the bag.

[0017] Furthermore, the present application also provides the use of the above-mentioned Stropharia officinalis substitute culture medium in the cultivation of Stropharia officinalis.

[0018] Furthermore, the present application also provides the application of the method for preparing a Stropharia cap mushroom bag in Stropharia cap mushroom cultivation.

[0019] By implementing the technical solution of the present invention, the following beneficial effects can be achieved:

[0020] The invention provides a stropharia substitute culture medium and a method for preparing a mushroom bag. By developing new raw materials and formulas of the stropharia substitute culture medium, optimizing the method for preparing the mushroom bag, and increasing the air permeability of the mushroom bag, a formula of the stropharia substitute culture medium and a method for preparing the mushroom bag that are very beneficial to the growth of stropharia hyphae are screened. By using the culture medium formula and the method for preparing the mushroom bag of the invention, the stropharia strain culture time is only 20-30 days, which can be shortened by 10-20 days compared with the conventional strain culture time, the mycelium growth speed is significantly improved, and the mycelium density and growth potential are also significantly increased, thereby providing strong technical support for the safe and efficient stropharia strain production. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Shown is the growth rate trend of Stropharia rugosotis mycelium on culture media with different material-to-water ratios.

[0022] Figure 2 Shown is the growth status of Stropharia rugosotis mycelium on culture media with different material-water ratios.

[0023] Figure 3 Shown is a diagram of a new method for preparing mushroom bags for Stropharia rugosotis. Specific embodiments

[0024] The following examples are used to further illustrate the present invention, but should not be construed as limiting the present invention. Without departing from the spirit and substance of the present invention, modifications or substitutions made to the methods, steps or conditions of the present invention are within the scope of the present invention.

[0025] The reagents used in this application are: licorice residue, corn cobs, corn flour, glucose, potassium dihydrogen phosphate, magnesium sulfate, calcium hydroxide, PD liquid culture medium, and the commonly used reagents are domestic analytical grade reagents.

[0026] The instruments used in this application include: constant temperature water bath, high pressure sterilizer, constant temperature incubator, constant temperature shaker.

[0027] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following examples are all commercially available unless otherwise specified.

[0028] Example 1: A stropharia substitute culture medium

[0029] A stropharia substitute culture medium, wherein the formula of the substitute culture medium comprises, by weight percentage, 10%-94.6% of liquorice residue, 0%-84.6% of corn cobs, 2% of corn flour, 2% of glucose, 0.3% of potassium dihydrogen phosphate, 0.1% of magnesium sulfate, and 1% of calcium hydroxide.

[0030] Example 2: A stropharia substitute culture medium

[0031] A stropharia substitute culture medium, wherein the formula of the substitute culture medium comprises, by weight percentage, 10% liquorice residue, 84.6% corn cobs, 2% corn flour, 2% glucose, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and 1% calcium hydroxide.

[0032] Example 3: A stropharia substitute culture medium

[0033] A stropharia substitute culture medium, wherein the formula of the substitute culture medium comprises, by weight percentage, 20% liquorice residue, 74.6% corn cobs, 2% corn flour, 2% glucose, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and 1% calcium hydroxide.

[0034] Example 4: A Stropharia officinalis substitute culture medium

[0035] A stropharia substitute culture medium, wherein the formula of the substitute culture medium comprises, by weight percentage, 30% liquorice residue, 64.6% corn cobs, 2% corn flour, 2% glucose, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and 1% calcium hydroxide.

[0036] Example 5: A Stropharia officinalis substitute culture medium

[0037] A stropharia substitute culture medium, wherein the formula of the substitute culture medium comprises, by weight percentage, 40% liquorice residue, 54.6% corn cobs, 2% corn flour, 2% glucose, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and 1% calcium hydroxide.

[0038] Example 6: A Stropharia officinalis substitute culture medium

[0039] A stropharia substitute culture medium, wherein the formula of the substitute culture medium comprises, by weight percentage, 50% liquorice residue, 44.6% corn cobs, 2% corn flour, 2% glucose, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and 1% calcium hydroxide.

[0040] Example 7: A Stropharia officinalis substitute culture medium

[0041] A stropharia substitute culture medium, wherein the formula of the substitute culture medium comprises, by weight percentage, 60% liquorice residue, 34.6% corn cobs, 2% corn flour, 2% glucose, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and 1% calcium hydroxide.

[0042] Example 8: A Stropharia officinalis substitute culture medium

[0043] A stropharia substitute culture medium, wherein the formula of the substitute culture medium comprises, by weight percentage, 70% liquorice residue, 24.6% corn cobs, 2% corn flour, 2% glucose, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and 1% calcium hydroxide.

[0044] Example 9: A Stropharia officinalis substitute culture medium

[0045] A stropharia substitute culture medium, wherein the formula of the substitute culture medium comprises, by weight percentage, 80% of liquorice residue, 14.6% of corn cobs, 2% of corn flour, 2% of glucose, 0.3% of potassium dihydrogen phosphate, 0.1% of magnesium sulfate, and 1% of calcium hydroxide.

[0046] Example 10: A Stropharia officinalis substitute culture medium

[0047] A stropharia substitute culture medium, wherein the formula of the substitute culture medium comprises, by weight percentage, 94.6% liquorice residue, 2% corn flour, 2% glucose, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and 1% calcium hydroxide.

[0048] Example 11: A method for preparing a stropharia bag

[0049] Pre-wet fresh, non-moldy licorice residue and corn cobs with tap water for more than 12 hours in advance to make them completely soaked, and then mix all the ingredients of corn flour, glucose, potassium dihydrogen phosphate, magnesium sulfate and calcium hydroxide evenly according to the formula ratio; adjust the culture medium with tap water, the material-water ratio is 1:1.5-2.4, the water content is 60-70%, the pH value of the culture medium is tested, and the pH value is preferably between 9 and 11. After the culture medium is prepared, it is bagged in time and cannot be left overnight; the culture medium is packaged, and a high-temperature and high-pressure angle bag with a specification of 13-18cm×23-38cm×0.0025-0.004cm is selected. When bagging, the culture medium is loaded into the bag, the culture medium is compacted to a moderate degree of compactness, and then the ring is put on and the cover is buckled, leaving a large space in the upper half of the bag, and the loaded bag is sterilized with high-pressure steam at 121°C for 2.5-3 hours. After the sterilization is completed, inoculate in time when the bag temperature drops below 30°C.

[0050] Example 12: A method for preparing a stropharia bag

[0051] Pre-wet fresh, non-moldy licorice residue and corn cobs with tap water for more than 12 hours in advance to make them completely soaked, and then mix all the ingredients of corn flour, glucose, potassium dihydrogen phosphate, magnesium sulfate and calcium hydroxide evenly according to the formula ratio; adjust the culture medium with tap water, the material-water ratio is 1:1.8, the water content is 60-70%, the pH value of the culture medium is tested, and the pH value is preferably between 9 and 11. After the culture medium is prepared, it is bagged in time and cannot be left overnight; the culture medium is packaged, and a high-temperature and high-pressure angle bag with a specification of 13-18cm×23-38cm×0.0025-0.004cm is selected. When bagging, the culture medium is loaded into the bag, the culture medium is compacted to a moderate degree of compaction, and then the ring is put on and the cover is buckled, leaving a large space in the upper half of the bag, and the loaded bag is sterilized by high-pressure steam at 121°C for 2.5-3 hours. After the sterilization is completed, inoculate in time when the bag temperature drops below 30°C.

[0052] Example 13: A method for preparing a stropharia bag

[0053] Pre-wet fresh, non-moldy licorice residue and corn cobs with tap water for more than 12 hours in advance to make them completely soaked, and then mix all the ingredients of corn flour, glucose, potassium dihydrogen phosphate, magnesium sulfate and calcium hydroxide evenly according to the formula ratio; adjust the culture medium with tap water, the material-water ratio is 1:2.0, the water content is 60-70%, the pH value of the culture medium is tested, and the pH value is preferably between 9 and 11. After the culture medium is prepared, it is bagged in time and cannot be left overnight; the culture medium is packaged, and a high-temperature and high-pressure angle bag with a specification of 13-18cm×23-38cm×0.0025-0.004cm is selected. When bagging, the culture medium is loaded into the bag, the culture medium is compacted to a moderate degree of compaction, and then the ring is put on and the cover is buckled, leaving a large space in the upper half of the bag, and the loaded bag is sterilized with high-pressure steam at 121°C for 2.5-3 hours. After the sterilization is completed, inoculate in time when the bag temperature drops below 30°C.

[0054] Example 14: A method for preparing a stropharia bag

[0055] Pre-wet fresh, non-moldy licorice residue and corn cobs with tap water for more than 12 hours in advance to make them completely soaked, and then mix all the ingredients of corn flour, glucose, potassium dihydrogen phosphate, magnesium sulfate and calcium hydroxide evenly according to the formula ratio; adjust the culture medium with tap water, the material-water ratio is 1:2.2, the water content is 60-70%, the pH value of the culture medium is tested, and the pH value is preferably between 9 and 11. After the culture medium is prepared, it is bagged in time and cannot be left overnight; the culture medium is packaged, and a high-temperature and high-pressure angle bag with a specification of 13-18cm×23-38cm×0.0025-0.004cm is selected. When bagging, the culture medium is loaded into the bag, the culture medium is compacted to a moderate degree of compaction, and then the ring is put on and the cover is buckled, leaving a large space in the upper half of the bag, and the loaded bag is sterilized by high-pressure steam at 121°C for 2.5-3 hours. After the sterilization is completed, inoculate in time when the bag temperature drops below 30°C.

[0056] Example 15: A method for preparing a stropharia bag

[0057] Pre-wet fresh, non-moldy licorice residue and corn cobs with tap water for more than 12 hours in advance to make them completely soaked, and then mix all the ingredients of corn flour, glucose, potassium dihydrogen phosphate, magnesium sulfate and calcium hydroxide evenly according to the formula ratio; adjust the culture medium with tap water, the material-water ratio is 1:2.4, the water content is 60-70%, the pH value of the culture medium is tested, and the pH value is preferably between 9 and 11. After the culture medium is prepared, it is bagged in time and cannot be left overnight; the culture medium is packaged, and a high-temperature and high-pressure angle bag with a specification of 13-18cm×23-38cm×0.0025-0.004cm is selected. When bagging, the culture medium is loaded into the bag, the culture medium is compacted to a moderate degree of compaction, and then the ring is put on and the cover is buckled, leaving a large space in the upper half of the bag, and the loaded bag is sterilized by high-pressure steam at 121°C for 2.5-3 hours. After the sterilization is completed, inoculate in time when the bag temperature drops below 30°C.

[0058] Example 16: Optimization of the formula of the stropharia substitute culture medium and the preparation method of the mushroom bag

[0059] 1. Formula of culture medium for Agaricus bisporus

[0060] Licorice residue 10-94.6%, corn cob 0-84.6%, corn flour 2%, glucose 2%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.1%, calcium hydroxide 1%, water content 60-70%, pH 7-8.

[0061] Note: The size of the corn cob particles used is 0.5-1cm×0.5-1cm and needs to be pre-wetted 12 hours in advance.

[0062] 2. Preparation method of Stropharia officinalis original culture medium

[0063] (1) Culture medium preparation and sterilization

[0064] Pre-wet fresh, mold-free licorice residue and corn cobs with tap water for more than 12 hours to fully saturate them, then mix all the ingredients evenly according to the formula ratio, adjust the moisture content of the culture medium to 60-70% with tap water, test the pH value of the culture medium, and make sure the pH value is between 9 and 11. After the culture medium is prepared, bag it in time and do not leave it overnight.

[0065] Choose high temperature and high pressure resistant angled bags with specifications of 13-18cm×23-38cm×0.0025-0.004cm. When bagging, fill the culture medium to 1 / 3 to 2 / 3 of the bag volume, compact the culture medium to a moderate degree of compaction, then put on the ring and cover, leaving a large space in the upper part of the bag, sterilize the filled bag with high pressure steam at 121℃ for 2.5-3 hours, and after sterilization, wait until the bag temperature drops below 30℃ and inoculate in time.

[0066] (2) Inoculation and culture

[0067] The liquid seed inoculation method is adopted. First, under sterile conditions, the mycelium blocks of Stropharia capensis (i.e., 3 to 4 colonies with a diameter of 7 to 8 cm) are inoculated into the PD liquid culture medium, antibiotics are added, and then the liquid strains are prepared by using a stirrer; the strains are shaken and inoculated into the culture medium in the sterilized material bag, with an inoculation volume of 10 to 20 ml per bag; and then the strains are cultured in the dark in a constant temperature box at 23°C, and the mycelium growth is observed and recorded every day.

[0068] 3. Screening experiment of substitute culture medium formula

[0069] (1) Culture medium formula design: Licorice residue and corn cobs were used as the main ingredients, and different weight ratios were set as experimental treatments. A total of 9 culture medium formulas were designed; the formula with corn cobs and rice husks as the main ingredients was used as the control (CK), which is a conventional formula currently used with good results; the names and proportions of each component are detailed in Table 1.

[0070] Table 1: Formula of Stropharia officinalis substitute medium

[0071]

[0072] (2) Result analysis:

[0073] The results are shown in Table 2: (1) In terms of mycelium growth rate, the mycelium growth rate of all formulations was in the range of 1.27-5.01 mm / d, among which the mycelium growth rate of the control (CK) group formulation was 2.66 mm / d, which was significantly higher than that of formulations 1 and 2, but significantly lower than that of formulations 3-9; (2) In terms of mycelium density, CK and formulations 1 and 2 were all at a relatively dense level, and formulations 3-9 were all at a dense level.

[0074] In summary, the introduction of licorice residue into the formula of conventional stropharia substitute medium can significantly promote the growth rate of stropharia mycelium, and has the effect of promoting the density and growth of stropharia mycelium, and the effect is very significant. At the same time, licorice residue can completely replace rice husks, providing more options for areas where rice husks are scarce.

[0075] Table 2: Mycelial growth of Stropharia rugosa substitute culture medium with different formulations

[0076]

[0077] Note: (1) The letters after the hyphae growth rate represent significant differences at p = 0.05; (2) Mycelium density classification, "+" means sparse, "++" means dense, and "+++" means dense;

[0078] 4. Experiment on optimizing water content of culture medium

[0079] (1) Experimental design of moisture content of culture medium: A total of 5 gradients of material-water ratio were set, from low to high, namely 1 / 1.5 (CK), 1 / 1.8, 1 / 2.0, 1 / 2.2, and 1 / 2.4, where CK is the conventional material-water ratio of edible fungus substitute culture medium; the culture medium formula used in the experiment was: 60% licorice residue, 34.6% corn cobs, 2% corn flour, 2% glucose, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and 1% calcium hydroxide; the culture medium was prepared according to the culture medium formula and material-water ratio, stirred and mixed thoroughly, and the pH was adjusted to 9-10. When bottling, the dry material amount and volume of the culture medium in each bottle were ensured to be consistent, and the filling amount was 2 / 3 of the bottle volume, and sterilized by high-pressure steam at 121°C for 2.5 hours.

[0080] (2) Results and analysis

[0081] The results showed that the mycelium growth rate increased with the increase of water content in the culture medium. Figure 1 , Attachment Figure 2 The mycelial growth rate was between 3.96-7.51 mm / d, as shown in Table 3. Among them, the growth rate was the slowest on the conventional medium with a feed-water ratio of 1 / 1.5 (CK), which was 3.96 mm / d, significantly lower than other feed-water ratios. Therefore, an appropriate increase in the water content of the substitute medium is beneficial to the growth of the mycelium of Stropharia rugosa.

[0082] Table 3: Growth of Stropharia rugosodium mycelium on culture media with different material-water ratios

[0083] Serial number Material-water ratio Mycelium growth rate (mm / d) Mycelium density Remark 1 1 / 1.5(CK) <![CDATA[3.96 a ]]> +++ 2 1 / 1.8 <![CDATA[4.26 ab ]]> +++ 3 1 / 2.0 <![CDATA[4.53 b ]]> +++ 4 1 / 2.2 <![CDATA[7.32 c ]]> +++ 5 1 / 2.4 <![CDATA[7.51 c ]]> +++

[0084] Based on the above experimental results, the formula of the substitute culture medium and the weight percentage of each component are: 94.6% licorice residue, 2% glucose, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and 1% calcium hydroxide. Combined with a specific method for preparing mushroom bags: mix the raw materials evenly according to the culture medium formula, add water and stir to fully mix, the material-water ratio is 1:2.2, and when bagging, fill the culture medium to no more than 2 / 3 of the volume of the mushroom bag, compact the culture medium, and the compaction degree is moderate, then put on the ring and buckle the lid, leaving a large space in the upper half of the mushroom bag, and sterilize the material bag with high-pressure steam at 121°C for 2.5-3 hours. Under the conditions of the relevant substitute culture medium and mushroom bag preparation method, it can provide suitable nutritional conditions and breathable space environment for the growth of Stropharia rugosa mycelium, and can significantly improve the growth rate, density and growth potential of Stropharia rugosa mycelium, improve the quality of the strain, and provide favorable technical support for the safe and efficient seed production of Stropharia rugosa.

[0085] Example 17: Optimization of the formula of the stropharia substitute culture medium and the preparation method of the mushroom bag

[0086] Based on the clear records in the above-mentioned Examples 1 to 16, the conventional method for preparing the mushroom bag with the culture medium base of the Stropharia capensis was used for cultivation as the control group, and the substitute culture medium formula in Example 10 of this application was combined with the method for preparing the mushroom bag in Example 14, and the filled material bags were sterilized with high-pressure steam at 121°C for 2.5-3 hours. After the sterilization, the temperature of the mushroom bag dropped to below 30°C and inoculated in time; liquid bacteria were used for inoculation, the inoculation volume was 15mL, and the culture was dark in an incubator at 25°C. As the experimental group, the time to fill the bag was compared between the two. The results showed that the time to fill the bag with the culture medium using this method was 25-30 days, which was about 15 days shorter than that of filling the bag with the culture medium without leaving any space.

[0087] As described above, the present invention can be well implemented. The above embodiments are only descriptions of the preferred implementation modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various changes and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the protection scope determined by the present invention.

Claims

1. A method for preparing a stropharia bag, characterized in that: The Stropharia rugosa mushroom bag is prepared by the following steps: (1) pre-wetting fresh, non-moldy licorice residue with tap water for more than 12 hours to make it completely soaked, and then mixing corn flour, glucose, potassium dihydrogen phosphate, magnesium sulfate, and calcium hydroxide according to the formula ratio; (2) adjusting the water content of the culture medium to 60-70% with tap water, testing the pH of the culture medium, and making sure that the pH value is between 9 and 11. After the culture medium is prepared, bag it in time and do not store it overnight; (3) The culture medium in step (2) is packaged, and a high temperature and high pressure resistant angled bag with a specification of 13-18 cm×23-38 cm×0.0025-0.004 cm is selected. When bagging, the culture medium is loaded into the bag, compacted to a moderate degree of compaction, and then the bag is covered with a ring, leaving a large space in the upper half of the bag, and the bag is sterilized with high pressure steam at 121° C. for 2.5-3 hours. After the sterilization is completed, inoculate the bag in time when the temperature of the bag drops below 30° C.; The feed medium formula is composed of the following components by weight percentage: licorice residue 94.6%, corn flour 2%, glucose 2%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.1%, calcium hydroxide 1%; The mixed components are adjusted with tap water to have a water content of the culture medium at a material-water ratio of 1:2.

2. When bagging, the culture medium is loaded into the bag to a volume not exceeding 2 / 3 of the bag volume.

2. A stropharia substitute culture medium, characterized in that: The method for preparing a Stropharia cap mushroom bag according to claim 1 is used to prepare the same.

3. Use of the Stropharia officinalis substitute culture medium as claimed in claim 2 in Stropharia officinalis cultivation.

4. Application of the method for preparing a Stropharia cap mushroom bag as claimed in claim 1 in Stropharia cap mushroom cultivation.

Images