A solution for preventing and treating dairy cow hoof disease and preparation method thereof

Abstract

The invention discloses a solution for preventing and treating hoof disease of dairy cows, and belongs to the technical field of veterinary disinfectants. The solution is composed of plant extracts, bactericides, hoof care agents, methyl salicylate, emollients, synergists, toners, and deionized water, and has good bactericidal, blood coagulation and detumescence effects, can effectively kill infectious pathogens of hoof disease of dairy cows, and promote keratin recovery. The solution of the invention does not contain antibiotics, copper sulfate, and strong oxidizing components, avoids irritation to the skin, is biodegradable and environmentally friendly; the preparation process is simple, the raw materials are simple and easy to obtain, and can be industrially produced.

Description

Technical Field

[0001] The invention belongs to the technical field of veterinary disinfectants, and particularly relates to a solution for preventing and treating cow hoof diseases and a preparation method thereof. Background Art

[0002] Hoof rot is a common disease that affects the health of dairy cows. The incidence rate is high, ranging from 30-50% in summer. In the death culling, hoof rot accounts for up to 40%, which seriously affects the health of dairy cows and reduces the useful life of dairy cows. In severe cases, cows die due to sepsis caused by worsening systemic symptoms. The Chinese government has introduced supportive regulations and policies, such as the Outline of the Plan for the Rectification and Revitalization of the Dairy Industry, to encourage the development of large-scale ranches. This has promoted larger-scale dairy farming, and more and more dairy cows are placed on large ranches.

[0003] The most commonly used dairy cow hoof baths in the domestic market are copper sulfate and formaldehyde. Copper sulfate is highly corrosive, has serious environmental hazards, and has average therapeutic effects. As the country's environmental protection efforts increase, it is gradually being eliminated. Formaldehyde solution is volatile and neurotoxic, with low safety, and many ranches have experienced accidents of human poisoning or even death due to formaldehyde. In addition, many places have banned the use of formaldehyde for dairy cow hoof baths.

[0004] At present, the prevention and control scheme for the hoof disease of dairy cows mainly includes adjusting and optimizing the summer diet formula, preventing malnutrition, strengthening the sanitary management of cowsheds, and timely repairing the hoof of cattle. Among them, it is the most common method to regularly soak the hoof of cattle in medicinal baths to prevent and treat the rot of cattle hoofs. Patent CN108606978A discloses a kind of hoof bathing liquid for dairy cows. Polyethylene glycol is used as a film-forming agent in the invention, and a long-term protective effect is played after the film formation of the hoof bathing liquid. However, due to the non-biodegradability of polyethylene glycol, it has a certain impact on the environment. In addition, long-term use of polyethylene glycol can cause dry skin, which may affect the treatment effect and reduce the efficacy of hoof bathing liquid in the process of treating hoof disease. Patent CN107837398A discloses a kind of composite hoof bathing liquid for dairy cows, which contains glutaraldehyde components in the components, and long-term use will have the appearance of drug-resistant strains to a large extent. At the same time, glutaraldehyde is unstable, and it is placed at room temperature for 2 weeks, and the concentration is significantly reduced. And the irritation of glutaraldehyde gas is large, and allergic dermatitis phenomenon may occur on the skin of cattle, which has a certain impact on the prevention and treatment effect. In addition, the patent also discloses the use of aluminum salt as an astringent, which is mainly used to repair and promote rapid wound healing. However, aluminum salt can acidify the soil and cause certain harm to the environment.

[0005] Therefore, it is very necessary to develop a safe and efficient sterilization solution that can significantly prevent and treat hoof diseases in dairy cows. Summary of the invention

[0006] In order to solve the problems in the prior art, the present invention proposes a solution for preventing and treating hoof diseases in dairy cows. The plant extract contained in the solution of the present invention can be compounded with a bactericide to enhance bactericidal and hoof protection, and it is a broad-spectrum bactericidal agent. The solution can effectively prevent hoof diseases in dairy cows in daily use, and can be used to relieve pain and promote blood circulation and remove blood stasis, control the condition, and reduce the pain caused by the disease when the dairy cow is sick; the solution of the present invention does not contain antibiotics, copper sulfate, and strong oxidizing components, avoids irritation to the skin, is biodegradable and environmentally friendly; the preparation process is simple, the raw materials are simple and easy to obtain, and can be industrially produced.

[0007] To achieve the above purpose, the specific technical solution of the present invention is as follows:

[0008] The invention provides a solution for preventing and treating hoof diseases of dairy cows. The components in the solution are plant extracts, bactericides, hoof protectors, methyl salicylate, emollients, synergists, pH regulators, toners and deionized water.

[0009] The solution for preventing and treating hoof disease of dairy cows comprises, by weight, 3-15 parts of plant extract, 1-10 parts of bactericide, 5-20 parts of hoof protector, 1-40 parts of methyl salicylate, 10-30 parts of emollient, 0.2-1 parts of synergist, 1-6 parts of toner and 30-80 parts of deionized water.

[0010] Preferably, the components of the solution for preventing and treating hoof disease in dairy cows include 8-15 parts of plant extract, 1-5 parts of fungicide, 11-16 parts of hoof protector, 1-6 parts of methyl salicylate, 15-22 parts of emollient, 0.2-1 parts of synergist, 1-5 parts of toner and 35-50 parts of deionized water by weight.

[0011] More preferably, the components of the solution for preventing and treating hoof disease in dairy cows include, by weight, 10 parts of plant extract, 2 parts of fungicide, 15 parts of hoof protector, 3 parts of methyl salicylate, 20 parts of emollient, 0.5 parts of synergist, 3 parts of toner, and 49.5 parts of deionized water.

[0012] The plant extract is one or more of thymol, cinnamaldehyde, pollen typhae extract, clove extract, motherwort extract, frankincense extract, zedoaria extract and calendula officinalis extract.

[0013] Preferably, the plant extract is one or more of cinnamaldehyde, pollen typhae extract and motherwort extract.

[0014] More preferably, the plant extract is one or both of cinnamaldehyde and motherwort extract.

[0015] The bactericide is one or more of nano silver solution, didecyl dimethyl propionic acid, hydroxyethyl quaternary ammonium salt, and gemini quaternary ammonium salt.

[0016] Preferably, the bactericide is one or both of nanosilver solution and hydroxyethyl quaternary ammonium salt, and more preferably, the bactericide is nanosilver.

[0017] The hoof care agent is one or more of amino acid chelated copper, amino acid chelated zinc, EDTA copper and EDTA zinc.

[0018] The preferred hoof care agent is one or both of EDTA copper and EDTA zinc.

[0019] The emollient is one or more of glycerin, sorbitol, honey, hyaluronic acid, gelatin, propylene glycol, water-soluble silicone oil, and water-soluble olive oil.

[0020] Preferably, the emollient is one or more of glycerin, sorbitol and hyaluronic acid.

[0021] The auxiliary agent is one or more of soybean lecithin, polysorbate-60, methyl glucose sesquistearate, sodium stearoyl glutamate and glucoside.

[0022] The preferred auxiliary agent is one or more of sodium stearoyl glutamate and glucoside, and more preferably sodium stearoyl glutamate.

[0023] The toner includes one or more of brilliant blue, acid blue, lemon yellow, and alizarin cyanine green, preferably brilliant blue.

[0024] The method for preparing the solution for preventing and treating cow hoof disease comprises the following steps:

[0025] (1) dissolving the above components in deionized water to prepare solution 1;

[0026] (2) filtering solution 1 to convert it into a clear solution, namely solution 2;

[0027] (3) adding a pH adjuster and fully dissolving to obtain a third solution, wherein the pH value of the solution is 2-5;

[0028] (4) adding a colorant, stirring evenly and then fixing the volume to obtain the solution for preventing and treating hoof disease in dairy cows.

[0029] The pH regulator includes one or more of acetic acid, boric acid, phosphoric acid, citric acid, hydrochloric acid and sodium hydroxide.

[0030] Preferably, the pH value of the solution in step (3) is 2.5-4.

[0031] The thymol in the solution for preventing and treating cow hoof disease is a monoterpene phenol compound with a special fragrance and obvious effects in antifungal, antioxidation, anti-inflammatory and antibacterial effects.

[0032] Cinnamaldehyde is an aldehyde compound with bactericidal, disinfectant and antiseptic properties, especially against fungi, and is anti-ulcer and antiviral.

[0033] Clove extract belongs to the clove buds of the myrtle plant, which are slightly ground into sticks. The corolla is spherical, with 4 petals, imbricate and clasped, brown to yellowish brown, and the petals contain stamens and styles. After crushing, many yellow granular anthers can be seen. The calyx tube is cylindrical, slightly flat, and some are slightly curved, reddish brown or brown, with 4 triangular sepals on the upper part, separated in a "cross" shape. It is solid and oily. It has antibacterial, analgesic and anti-inflammatory effects.

[0034] Typhae pollen extract is the pollen of Typha longibracteata, Typha angustifolia, Typha latifolia or various plants of the same genus in the Typhaceae family. The plant morphology is detailed in the entry for "Typhae". It has the effects of hemostasis, blood stasis and blood coagulation.

[0035] Motherwort extract is the above-ground part of the Lamiaceae plant Leonurus japonicus, which has the functions of invigorating blood circulation, promoting regeneration, clearing away heat and reducing swelling.

[0036] Frankincense extract is a yellow-white powdery fluid extract of the resin of the Oleaceae plant Boswellia carlesii. It has the effects of promoting blood circulation, relieving pain, reducing swelling and promoting tissue regeneration.

[0037] Ze Lan extract is made from the tender stems and leaves of sweet potato seedlings of the Lamiaceae family. It has the effects of promoting blood circulation, removing blood stasis, promoting diuresis and reducing swelling.

[0038] Calendula officinalis extract is an extract from the petals of the marigold plant of the Asteraceae family. It has anti-aging and antibacterial effects, especially against staphylococci and streptococci. The water extract of the leaves has the effect of accelerating blood coagulation (shortening the prothrombin time).

[0039] Nanosilver solution uses physical disinfection, does not produce harmful gases, has a spectrum of bactericidal effects, is non-irritating to the environment, has strong permeability, and its antibacterial effect becomes increasingly stronger when it comes into contact with water. It is beneficial for the treatment of cattle hoof disease and does not produce drug resistance, which can effectively avoid attacks caused by drug resistance and long-term treatment.

[0040] Methyl salicylate is generally used to relieve pain in local soft tissue inflammation. It has anti-inflammatory and analgesic effects. It can be used in dairy cow hoof baths to assist in the treatment process. While using the bath to sterilize, it can also relieve the pain of sick cows and promote local blood circulation.

[0041] Trace amounts of copper, zinc, etc. are the most critical mineral elements in the keratinization process of cattle hooves. They can improve the body's physical fitness and enhance the ability to resist pathogens.

[0042] Surfactants such as sodium stearoyl glutamate are selected as auxiliary agents. This ingredient is an amino acid of a surfactant. During use, it not only has a good penetration effect, but also can play a role in cleaning and foaming. It is very suitable for the cow's hoof area with thin and sensitive stratum corneum.

[0043] The solution for preventing and treating cow hoof diseases of the present invention can be a hoof bath liquid or an external spray or a smear.

[0044] Beneficial effects:

[0045] (1) The present invention provides a solution for preventing and treating hoof disease in dairy cows, which adopts a nano-silver solution and a plant extract to enhance the synergy, has good bactericidal, coagulation and detumescence effects, can effectively kill the infectious pathogens of hoof disease in dairy cows, and promote the recovery of cuticle.

[0046] (2) The present invention contains methyl salicylate, which has good analgesic and anti-inflammatory functions, relieves the pain of sick cattle, and is beneficial to the prevention and treatment of cattle hoof diseases.

[0047] (3) The present invention does not contain glutaraldehyde and antibiotics, is non-irritating and non-carcinogenic to the skin, does not produce drug resistance, and is harmless to the environment.

[0048] (4) The preparation method of the present invention has simple process, readily available raw materials, and is easy to industrialize. DETAILED DESCRIPTION

[0049] The above contents will be further described in detail below through specific implementation methods in the form of embodiments.

[0050] The solutions for preventing and treating cow hoof diseases in the present invention include but are not limited to the following:

[0051] Example 1

[0052] A solution for preventing and treating hoof disease of dairy cows, comprising the following raw materials by weight: 4 parts of cinnamaldehyde, 6 parts of motherwort, 2 parts of nano silver ion solution, 7 parts of EDTA copper, 8 parts of EDTA zinc, 3 parts of methyl salicylate, 20 parts of glycerol, 0.5 parts of sodium stearoyl glutamate, and 3 parts of brilliant blue. Preparation method: Add the above materials, dissolve in 49.5 parts of ionized water, filter, add acetic acid to adjust pH, stir evenly, and dilute to 100 parts. The pH of the bath solution is 2.7.

[0053] Example 2

[0054] A solution for preventing and treating hoof disease of dairy cows, comprising the following raw materials by weight: 5 parts of cinnamaldehyde, 5 parts of motherwort, 2 parts of hydroxyethyl quaternary ammonium salt solution, 9 parts of EDTA copper, 6 parts of EDTA zinc, 3 parts of methyl salicylate, 20 parts of glycerol, 0.5 parts of sodium stearoyl glutamate, and 3 parts of brilliant blue. Preparation method: Add the above materials, dissolve in 49.5 parts of ionized water, filter, add acetic acid to adjust the pH, stir well, and dilute to 100 parts. The pH of the bath solution is 2.8.

[0055] Example 3

[0056] A solution for preventing and treating hoof disease of dairy cows, comprising the following raw materials by weight: 10 parts of thymol, 2 parts of hydroxyethyl quaternary ammonium salt solution, 3 parts of amino acid chelated copper, 10 parts of amino acid chelated zinc, 3 parts of methyl salicylate, 15 parts of glycerol, 0.5 parts of sodium stearoyl glutamate, and 3 parts of brilliant blue. Preparation method: Add the above materials, dissolve in 50 parts of ionized water, filter, add acetic acid to adjust pH, stir well, and dilute to 100 parts. The pH of the bath solution is 3.2.

[0057] Example 4

[0058] A solution for preventing and treating hoof disease of dairy cows, comprising the following raw materials by weight: 5 parts of cinnamaldehyde, 7 parts of motherwort extract, 2 parts of nano silver solution, 3 parts of amino acid chelated copper, 10 parts of amino acid chelated zinc, 15 parts of hyaluronic acid, 0.5 parts of sodium stearoyl glutamate, and 3 parts of brilliant blue. Preparation method: Add the above materials, dissolve in 40 parts of ionized water, filter, add acetic acid to adjust the pH, stir well, and dilute to 100 parts. The pH of the bath solution is 2.5.

[0059] Example 5

[0060] A solution for preventing and treating hoof disease of dairy cows, comprising the following raw materials by weight: 15 parts of calendula extract, 2 parts of nano-silver solution, 3 parts of EDTA copper, 8 parts of EDTA zinc, 2 parts of methyl salicylate, 15 parts of hyaluronic acid, 0.5 parts of sodium stearoyl glutamate, and 3 parts of brilliant blue. Preparation method: Add the above materials, dissolve in 35 parts of ionized water, filter, add acetic acid to adjust pH, stir evenly, and dilute to 100 parts. The pH of the bath solution is 3.2.

[0061] Example 6

[0062] A solution for preventing and treating hoof disease of dairy cows, comprising the following raw materials by weight: 6 parts of cinnamaldehyde, 4 parts of motherwort, 2 parts of nano silver ion solution, 10 parts of EDTA copper, 5 parts of EDTA zinc, 3 parts of methyl salicylate, 20 parts of glycerol, 0.5 parts of water-soluble azone, and 3 parts of brilliant blue. Preparation method: Add the above materials, dissolve in 49.5 parts of ionized water, filter, add acetic acid to adjust pH, stir well, and dilute to 100 parts. The pH of the bath solution is 3.6.

[0063] The operation and use method of the solution for preventing and treating hoof diseases of dairy cows of the present invention is as follows: taking the solution for preventing and treating hoof diseases of dairy cows, diluting it 50 times for use.

[0064] Example 7 Bactericidal effect verification

[0065] Sampling in the cattle farm, select cows with the same degree of hoof rot for sampling, lift and fix the affected limbs, and wash the affected areas with water. After using the solution for preventing and treating hoof disease in dairy cows in Examples 1-6 of the present invention, use a disposable cotton swab to dip different positions of the lesion tissue 5 times, put it into a sterilized EP tube, add an equal amount of physiological saline, and store it at low temperature. The bacterial solution was shaken and diluted (1:9), 100 μl of the dilution was drawn and added dropwise to the plate (anaerobic broth culture medium and blood agar culture medium prepared in advance), and evenly spread with a triangular spreading rod. The plates were placed in a 37°C constant temperature incubator and an anaerobic incubator and cultured for 48 hours. The plate colony counting method was used to perform statistics based on the test results. The laboratory recorded the bacteria on the hooves of 48 dairy cows before and after treatment (8 in each group), and the results are shown in the following table.

[0066] Table 1 Bacterial count results before and after treatment in different embodiments (n=8)

[0067]

[0068] According to the results, it can be seen that Examples 1-6 have an inhibitory effect on hoof bacteria, and the number of bacteria after treatment is significantly lower than the number of bacteria before treatment. Among them, the bactericidal effect of Example 1 and Example 2 is more significant than that of Example 3 and Example 5, and the number of colonies is significantly reduced. It can be concluded that the plant extracts in Examples 3 and 5 are thymol and calendula, and after the bacterial counting method is performed, the treatment effect is not as good as the combined effect of cinnamaldehyde and motherwort. In Example 4, methyl salicylate was not added, and the number of bacteria after treatment was significantly higher than the formula with methyl salicylate added; in Example 6, the synergist is water-soluble azone, and the number of bacteria after treatment is significantly higher than the formula of the present invention in which the synergist is sodium stearoyl glutamate. Therefore, cinnamaldehyde and motherwort are selected as plant extracts, nanosilver is selected as bactericide, and methyl salicylate and synergist sodium stearoyl glutamate are combined to make the hoof bath liquid have a good bactericidal effect, and the bactericidal rate can reach 99%. Bactericidal rate = (1-number of colonies after treatment / number of colonies before treatment) × 100.

[0069] Example 8 Clinical effect test

[0070] In order to verify the efficacy of the solution for preventing and treating hoof diseases of dairy cows of the present invention, 1,400 cows with limb and hoof diseases were selected from a ranch in Henan Province, including 560 cases of hoof rot, 350 cases of interdigital hyperplasia, 280 cases of hoof ulcers, and 210 cases of interdigital dermatitis.

[0071] The same number of cows with hoof disease were selected from each type of cows with hoof disease as the experimental group, and the rest of the diseased cows were selected as the control group. The same volume of 8% copper sulfate solution was added to the hoof bath of the control group according to the use requirements.

[0072] Usage: According to the requirements of hoof disease treatment, the hooves of the selected dairy cows are now thoroughly cleaned. The test group is divided into 6 groups, and the hoof bathing liquid prepared by the 1-6 formulas in the implementation case is added to 6 hoof bathing pools. The hoof bathing liquid is diluted 50 times and then soaked for treatment. Each cow is bathed for 30 minutes each time, twice a day, and continuously bathed for 10 days. The treatment effect is observed and recorded on the 5th and 10th days respectively. The experimental results are shown in Tables 2 and 3.

[0073] Efficacy evaluation criteria: cured - lameness, pain, swelling and other symptoms disappear; effective - lameness is alleviated, pain is improved, and the swelling range is reduced; ineffective - lameness does not disappear, pain does not disappear, and swelling does not subside.

[0074] Table 2 Statistics of 5-day hoof bath results

[0075]

[0076] Table 3 Statistics of results of 10-day hoof bath

[0077]

[0078]

[0079] Through clinical trials, it can be concluded that the solution for preventing and treating hoof diseases of dairy cows of the present invention has a good therapeutic effect on hoof rot, interdigital hyperplasia, hoof ulcers and interdigital dermatitis of dairy cows, and has obvious therapeutic advantages compared with the copper sulfate solution used in pastures. And compared with the copper sulfate solution of the control group, the solution of the present invention uses compound plant extracts, nanosilver solution and methyl salicylate, and synergist compound, which not only has a greater advantage in therapeutic effect, but the use of methyl salicylate also plays an analgesic effect, reducing the pain of sick cows. In summary, the present invention is more advantageous than conventional copper sulfate solution in treating hoof diseases of cattle, and increases breeding benefits.

Claims

1. A solution for preventing and treating hoof disease in dairy cows, characterized in that: The solution is composed of the following raw materials in parts by weight: 4 parts of cinnamaldehyde, 6 parts of motherwort, 2 parts of nano silver ion solution, 7 parts of EDTA copper, 8 parts of EDTA zinc, 3 parts of methyl salicylate, 20 parts of glycerol, 0.5 parts of sodium stearoyl glutamate, and 3 parts of brilliant blue.

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