Composition containing hyaluronic acid or its salt for improving body immunity and promoting gastrointestinal health and application thereof

Abstract

The present invention discloses a hyaluronic acid or salt composition and its application for improving body immunity and protecting gastrointestinal health. The composition containing hyaluronic acid or salt thereof comprises three molecular weight hyaluronic acids or salts thereof. The composition of the present invention can enhance immunity, relieve intestinal inflammation, promote intestinal peristalsis, and regulate intestinal flora through synergistic enhancement, and can be added to food and health food as an effective ingredient, or used as an excipient in combination with drugs.

Description

Technical Field

[0001] The present invention relates to the technical field of food, health care products or medicine, and in particular to a composition of hyaluronic acid or its salt for improving body immunity and promoting gastrointestinal health and an application thereof. Background Art

[0002] Hyaluronic acid (HA) is an acidic mucopolysaccharide composed of repeated disaccharide units of D-glucuronic acid and N-acetylglucosamine, which is widely present in the interstitial tissue of animals and the capsule of some bacteria. At present, hyaluronic acid has been widely used in the fields of cosmetics and medicine. With the in-depth research and understanding of hyaluronic acid at home and abroad, its application in the food field, especially in functional food, has also shown extensive and unique value.

[0003] The sudden outbreak of COVID-19 has spread across the globe, and most countries have not yet achieved effective control of the epidemic. In this case, strengthening routine prevention and control and improving the body's own immune capacity have become the key to preventing COVID-19 and resisting diseases. Immunity is the body's own defense mechanism, which is the body's ability to identify and eliminate any foreign bodies (viruses, bacteria, etc.) that invade from outside, deal with damaged, aged, dead, and degenerated cells, and identify and deal with mutant cells and virus-infected cells in the body. Improving immunity is a physiological reaction of the human body to identify and exclude "foreigners". It can be enhanced in certain ways. In addition to exercise, you can also eat more beneficial foods, especially functional foods that regulate immune factors and improve resistance.

[0004] The gastrointestinal tract is the largest immune and detoxification organ in the human body and has a significant impact on health. More than 70% of the human immune cells are located on the gastrointestinal mucosa, which makes the intestines form an immune barrier to resist bacteria and viruses and maintain the stability of the intestinal environment. However, in recent years, the incidence of gastrointestinal diseases has become increasingly high, such as gastrointestinal inflammation, gastrointestinal stones, gastric ulcers, intestinal flora imbalance, etc. More and more people are suffering from intestinal diseases. Therefore, the development of raw materials and products that promote intestinal health has the functions of preventing and relieving intestinal inflammation, promoting intestinal peristalsis, and regulating intestinal flora, which can meet the needs of the market and people, and also have considerable economic benefits.

[0005] At present, people are more and more deeply studying HA. HA has a certain effect on improving human immunity as a functional raw material. The application of hyaluronic acid in improving human immunity and improving the symptoms of immunity deficiency in Chinese patent application CN1498626A introduces a composition with hyaluronic acid as the functional component. The product has no toxic effect and has the effect of improving immunity in mouse tests and human experiments. However, the specific molecular weight of hyaluronic acid is not described, and the function of hyaluronic acid with different molecular weights is not reflected, and the reference value is low. Chinese patent CN108498549B introduces a mixture of hyaluronic acid and lactic acid bacteria for improving immune function, which is used to alleviate and treat complications induced by anti-tumor chemotherapy, reduce the side effects of chemotherapy drugs, and improve the condition of low immunity. This method needs to act on lactic acid bacteria, and lactic acid bacteria need to be prepared into freeze-dried bacterial powder. The composition also needs to add other auxiliary materials, such as freeze-drying protective agent and molding agent. The operation is complicated, and there are many kinds of added substances, which fail to give full play to the effect of hyaluronic acid with different molecular weights.

[0006] In addition, HA also has a certain effect in promoting gastrointestinal health. Research from the University of Oklahoma Health Sciences Center shows that 35kDa HA can enhance epithelial barrier function and prevent the occurrence of necrotizing enterocolitis in mice. There are also relevant domestic literature reports that in intestinal infections of mice caused by pathogenic bacteria (Listeria, Citrobacter rodentium, enteropathogenic Escherichia coli), the use of 530kDa hyaluronic acid treatment can play a protective role. These experiments use HA of a single molecular weight and have a single effect.

[0007] Therefore, in order to solve the problem of single efficacy of single molecular weight HA, the present invention intends to provide a HA complex and its products with clear ingredients and significant effects, so as to give full play to the role of hyaluronic acid in improving immunity and protecting gastrointestinal health. Summary of the invention

[0008] In view of the above problems, the present invention provides a composition of hyaluronic acid or its salts for improving the body's immunity and promoting gastrointestinal health. The composition is composed of hyaluronic acid or its salts with different molecular weights. According to the different functions of hyaluronic acid with different molecular weights, the composition can regulate immunity through synergistic enhancement, improve the ability to resist gastrointestinal inflammation, promote intestinal peristalsis and the growth of intestinal probiotics, etc.

[0009] The specific technical solutions of the present invention are as follows:

[0010] 1. A composition containing hyaluronic acid or its salt, comprising three molecular weight hyaluronic acids or their salts, wherein:

[0011] The molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa, preferably 10k-100kDa, and more preferably 30k-50kDa;

[0012] The molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-1000kDa, preferably 200k-600kDa, and more preferably 200k-400kDa;

[0013] The molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa, preferably 1300k-1500kDa.

[0014] 2. The composition of item 1, wherein the total number of parts after mixing is 10 parts by weight,

[0015] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0016] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0017] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0018] 3. The composition according to any one of items 1 to 2, wherein the hyaluronic acid or its salt is selected from one or more of hyaluronic acid, sodium hyaluronate, potassium hyaluronate, calcium hyaluronate and zinc hyaluronate, preferably sodium hyaluronate or potassium hyaluronate.

[0019] 4. Use of the composition described in any one of items 1 to 3 in the field of food, health products or medicine.

[0020] 5. A food composition comprising the composition described in any one of items 1 to 3.

[0021] 6. The food composition described in item 5, wherein the food composition is used to improve the body's immune ability, increase intestinal motility and / or regulate intestinal flora.

[0022] 7. A health care product composition, comprising the composition described in any one of items 1 to 3.

[0023] 8. The food composition described in item 7, wherein the health care product composition is used to improve the body's immune ability, increase intestinal motility and / or regulate intestinal flora.

[0024] 9. A pharmaceutical composition comprising the composition described in any one of items 1 to 3.

[0025] 10. The pharmaceutical composition according to item 9, wherein the pharmaceutical composition is used to enhance the body's immune capacity, relieve intestinal inflammation, increase intestinal motility and / or regulate intestinal flora.

[0026] Effects of the Invention

[0027] 1. The composition provided by the present invention has clear ingredients, simple operation, and is easy to prepare and use.

[0028] 2. The composition provided by the present invention has significant effects on improving the body's immunity, promoting intestinal peristalsis, the growth of intestinal probiotics and protecting gastric mucosa, and can be added to food and health food as an effective ingredient.

[0029] 3. The composition provided by the present invention has significant effects on improving the body's immunity, relieving gastrointestinal inflammation, promoting intestinal peristalsis, the growth of intestinal probiotics and protecting gastric mucosa, and can be used as an auxiliary material in combination with drugs. DETAILED DESCRIPTION

[0030] The present invention is described in detail below. Although specific embodiments of the present invention are shown, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0031] It should be noted that certain words are used in the specification and claims to refer to specific components. Those skilled in the art should understand that technicians may use different nouns to refer to the same component. This specification and claims do not use the difference in nouns as a way to distinguish components, but use the difference in the functions of the components as the criterion for distinction. As mentioned throughout the specification and claims, "including" or "comprising" are open-ended terms and should be interpreted as "including but not limited to". The subsequent description of the specification is a preferred embodiment of the present invention, but the description is based on the general principles of the specification and is not intended to limit the scope of the present invention. The scope of protection of the present invention shall be determined by the attached claims.

[0032] The present invention provides a composition containing hyaluronic acid or its salt, which comprises three hyaluronic acids or its salts with different molecular weights.

[0033] The molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa, preferably 10k-100kDa, and more preferably 30k-50kDa;

[0034] The molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-1000kDa, preferably 200k-600kDa, and more preferably 200k-400kDa; and

[0035] The molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa, preferably 1300k-1500kDa.

[0036] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-100kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-1000kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa.

[0037] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-100kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-600kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa.

[0038] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-100kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-600kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa.

[0039] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-100kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa.

[0040] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-100kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa.

[0041] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-100kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-1000kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa.

[0042] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-600kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa.

[0043] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-600kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa.

[0044] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-1000kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa.

[0045] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-1000kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa.

[0046] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa.

[0047] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa.

[0048] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 30k-50kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-1000kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa.

[0049] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 30k-50kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-1000kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa.

[0050] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 30k-50kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 20k-600kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa.

[0051] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 30k-50kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 20k-600kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa.

[0052] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 30k-50kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 20k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa.

[0053] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 30k-50kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 20k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa.

[0054] For example, the molecular weight of the first molecular weight hyaluronic acid or its salt can be 10 kDa, 20 kDa, 30 kDa, 40 kDa, 50 kDa, 60 kDa, 70 kDa, 80 kDa, 90 kDa, 100 kDa, 160 kDa, 200 kDa, etc.;

[0055] The molecular weight of the second molecular weight hyaluronic acid or its salt may be 200 kDa, 280 kD, 300 kDa, 400 kDa, 500 kDa, 600 kDa, 700 kDa, 800 kDa, 900 kDa, 1000 kDa, etc.;

[0056] The molecular weight of the third molecular weight hyaluronic acid or its salt can be 1000 kDa, 1100 kDa, 1200 kDa, 1300 kDa, 1350 kDa, 1400 kDa, 1500 kDa, etc.

[0057] In one embodiment, based on 10 parts by weight of the total number of parts after mixing,

[0058] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0059] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0060] For example, in parts by weight, the total number of parts after mixing is 10 parts,

[0061] The first molecular weight hyaluronic acid or its salt may be 1 part, 2 parts, 3 parts, 4 parts, etc.;

[0062] The second molecular weight hyaluronic acid or its salt can be 3 parts, 4 parts, 5 parts, 6 parts, etc.;

[0063] The third molecular weight hyaluronic acid or its salt can be 1 part, 2 parts, 3 parts, etc.

[0064] In the above specific embodiment, for example, 1-4 parts, preferably 2-4 parts, of 3 parts of a first molecular weight hyaluronic acid or a salt thereof, 3-6 parts, preferably 4-6 parts, of 5 parts of a second molecular weight hyaluronic acid or a salt thereof, and 1-3 parts of 2 parts of a third molecular weight hyaluronic acid or a salt thereof are weighed and mixed to obtain the composition containing hyaluronic acid or a salt thereof described herein.

[0065] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-100kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0066] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0067] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0068] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0069] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-100kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0070] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0071] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0072] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0073] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-100kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-600kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0074] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0075] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0076] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0077] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-100kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0078] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0079] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0080] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0081] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-100kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0082] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0083] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0084] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0085] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-100kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-1000kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0086] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0087] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0088] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0089] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-600kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0090] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0091] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0092] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0093] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-600kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0094] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0095] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0096] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0097] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-1000kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa, based on 10 parts by weight of the total number of parts after mixing.

[0098] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0099] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0100] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0101] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-1000kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0102] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0103] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0104] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0105] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0106] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0107] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0108] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0109] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 10k-200kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0110] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0111] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0112] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0113] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 30k-50kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-1000kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0114] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0115] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0116] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0117] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 30k-50kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 200k-1000kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0118] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0119] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0120] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0121] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 30k-50kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 20k-600kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0122] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0123] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0124] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0125] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 30k-50kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 20k-600kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0126] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0127] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0128] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0129] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three hyaluronic acids or their salts with different molecular weights, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 30k-50kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 20k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0130] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0131] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0132] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0133] In one embodiment, the present invention provides a composition containing hyaluronic acid or its salt, which comprises three different molecular weight hyaluronic acids or their salts, wherein the molecular weight of the first molecular weight hyaluronic acid or its salt is 30k-50kDa; the molecular weight of the second molecular weight hyaluronic acid or its salt is 20k-400kDa; and the molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa, based on 10 parts by weight of the total number of parts after mixing,

[0134] The first molecular weight hyaluronic acid or its salt is 1-4 parts, preferably 2-4 parts;

[0135] The second molecular weight hyaluronic acid or its salt is 3-6 parts, preferably 4-6 parts; and

[0136] The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

[0137] In one embodiment, the hyaluronic acid or its salt is selected from one or more of hyaluronic acid, sodium hyaluronate, potassium hyaluronate, calcium hyaluronate and zinc hyaluronate, preferably sodium hyaluronate or potassium hyaluronate.

[0138] The present invention provides application of the above-mentioned composition in the field of food, health care products or medicine.

[0139] By applying the above-mentioned composition to the field of food or health products, the body's immune ability can be improved, and intestinal peristalsis and the growth of intestinal probiotics can be promoted.

[0140] By applying the above-mentioned composition to the pharmaceutical field, the body's immune ability can be improved, gastrointestinal inflammation can be relieved, and intestinal motility and the growth of intestinal probiotics can be promoted.

[0141] The present invention provides a food composition, which comprises the above-mentioned composition.

[0142] In one embodiment, the food composition may further include auxiliary materials, such as preservatives, food coloring, flavoring agents, emulsifiers, spices, and the like.

[0143] The present invention provides a health care product composition, which comprises the above-mentioned composition.

[0144] In one embodiment, the health care product composition may further include auxiliary materials, such as preservatives, food coloring, flavoring agents, emulsifiers, spices, etc.

[0145] The present invention provides a pharmaceutical composition, which comprises the above-mentioned composition.

[0146] In one embodiment, the pharmaceutical composition may further include excipients, such as lubricants, adhesives, colorants, diluents, absorbents, and the like.

[0147] The dosage form of the above-mentioned food composition, health product composition, and pharmaceutical composition is not limited, and can be, for example, in solid form, semi-solid form, or liquid form.

[0148] Example

[0149] The present invention generally and / or specifically describes the materials and test methods used in the test. In the following examples, unless otherwise specified, % represents wt%, i.e., weight percentage. The reagents or instruments used without indicating the manufacturer are all conventional reagent products that can be obtained commercially, wherein the first molecular weight hyaluronic acid or its salt, the second molecular weight hyaluronic acid or its salt, and the third molecular weight hyaluronic acid or its salt are all provided by Bloomage Biotechnology Co., Ltd.

[0150] Example 1

[0151] A first molecular weight sodium hyaluronate with a molecular weight of 40 kDa, a second molecular weight sodium hyaluronate with a molecular weight of 280 kDa, and a third molecular weight sodium hyaluronate with a molecular weight of 1350 kDa are mixed at a mass ratio of 3:5:2, and mixed thoroughly for use.

[0152] Example 2

[0153] The first molecular weight sodium hyaluronate with a molecular weight of 40 kDa, the second molecular weight sodium hyaluronate with a molecular weight of 600 kDa and the third molecular weight sodium hyaluronate with a molecular weight of 1350 kDa are mixed in a mass ratio of 3:5:2, and mixed thoroughly for use.

[0154] Example 3

[0155] The first molecular weight sodium hyaluronate with a molecular weight of 40 kDa, the second molecular weight sodium hyaluronate with a molecular weight of 280 kDa and the third molecular weight sodium hyaluronate with a molecular weight of 1000 kDa are mixed in a mass ratio of 3:5:2, and mixed thoroughly for use.

[0156] Example 4

[0157] A first molecular weight sodium hyaluronate with a molecular weight of 160 kDa, a second molecular weight sodium hyaluronate with a molecular weight of 280 kDa, and a third molecular weight sodium hyaluronate with a molecular weight of 1350 kDa are mixed at a mass ratio of 3:5:2, and mixed thoroughly for use.

[0158] Example 5

[0159] The first molecular weight sodium hyaluronate with a molecular weight of 160 kDa, the second molecular weight sodium hyaluronate with a molecular weight of 600 kDa, and the third molecular weight sodium hyaluronate with a molecular weight of 1350 kDa are mixed in a mass ratio of 3:5:2, and mixed thoroughly for use.

[0160] Example 6

[0161] A first molecular weight sodium hyaluronate with a molecular weight of 160 kDa, a second molecular weight sodium hyaluronate with a molecular weight of 280 kDa, and a third molecular weight sodium hyaluronate with a molecular weight of 1000 kDa are mixed at a mass ratio of 3:5:2, and mixed thoroughly for use.

[0162] Example 7

[0163] The first molecular weight sodium hyaluronate with a molecular weight of 40 kDa, the second molecular weight sodium hyaluronate with a molecular weight of 600 kDa, and the third molecular weight sodium hyaluronate with a molecular weight of 1000 kDa are mixed in a mass ratio of 3:5:2, and mixed thoroughly for use.

[0164] Example 8

[0165] The first molecular weight sodium hyaluronate with a molecular weight of 160 kDa, the second molecular weight sodium hyaluronate with a molecular weight of 600 kDa, and the third molecular weight sodium hyaluronate with a molecular weight of 1000 kDa are mixed in a mass ratio of 3:5:2, and mixed thoroughly for use.

[0166] Example 9

[0167] A first molecular weight sodium hyaluronate with a molecular weight of 40 kDa, a second molecular weight sodium hyaluronate with a molecular weight of 280 kDa, and a third molecular weight sodium hyaluronate with a molecular weight of 1350 kDa are mixed at a mass ratio of 3:6:1, and mixed thoroughly for use.

[0168] Example 10

[0169] A first molecular weight sodium hyaluronate with a molecular weight of 40 kDa, a second molecular weight sodium hyaluronate with a molecular weight of 280 kDa, and a third molecular weight sodium hyaluronate with a molecular weight of 1350 kDa are mixed at a mass ratio of 2:5:3, and mixed thoroughly for use.

[0170] Embodiment 11

[0171] A first molecular weight sodium hyaluronate with a molecular weight of 40 kDa, a second molecular weight sodium hyaluronate with a molecular weight of 280 kDa, and a third molecular weight sodium hyaluronate with a molecular weight of 1350 kDa are mixed at a mass ratio of 1:3:1, and mixed thoroughly for use.

[0172] Comparative Example 1

[0173] The first type of sodium hyaluronate with a molecular weight of 40 kDa is dissolved in physiological saline according to the concentration required for each experiment, and is set aside after being completely dissolved.

[0174] Comparative Example 2

[0175] Sodium hyaluronate of the third molecular weight with a molecular weight of 1350 kDa is dissolved in physiological saline at the concentration required for each experiment and is set aside after being completely dissolved.

[0176] Comparative Example 3

[0177] Mix the first molecular weight sodium hyaluronate with a molecular weight of 40 kDa and the third molecular weight sodium hyaluronate with a molecular weight of 1350 kDa in a mass ratio of 3:2, mix well and set aside.

[0178] Comparative Example 4

[0179] Sodium hyaluronate of the second molecular weight of 280 kDa is dissolved in physiological saline at the concentration required for each experiment and is set aside after being completely dissolved.

[0180] Comparative Example 5

[0181] The second molecular weight sodium hyaluronate with a molecular weight of 280 kDa and the third molecular weight sodium hyaluronate with a molecular weight of 1350 kDa are mixed at a mass ratio of 5:2, and mixed thoroughly for use.

[0182] Comparative Example 6

[0183] Sodium hyaluronate with a molecular weight of 80 kDa is dissolved in physiological saline according to the concentration required for each experiment, and is set aside after being completely dissolved.

[0184] Comparative Example 7

[0185] Mix the first molecular weight sodium hyaluronate with a molecular weight of 40 kDa and the second molecular weight sodium hyaluronate with a molecular weight of 280 kDa in a mass ratio of 3:5, mix well and set aside.

[0186] Table 1 Dosage table of components used in Examples and Comparative Examples

[0187]

[0188] Experimental Materials

[0189] The different strains of zebrafish used in the experiment were provided by the Zebrafish Drug Screening Platform of the Institute of Biology, Shandong Academy of Sciences; the hyaluronic acid samples were provided by Bloomage Biotech Co., Ltd.

[0190] Instruments and Equipment

[0191] SZX16 fluorescence microscope and DP2-BSW image acquisition system (Olympus, Japan); Forma 3111 water-jacketed CO2 incubator (Forma, USA); zebrafish breeding equipment (Beijing Aisheng Technology Co., Ltd.)

[0192] Experimental Example 1: Immunity Regulation Experiment

[0193] Using the zebrafish strain Tg (lyz: GFP) as a model, after the fertilized eggs developed at 48 hpf (hour postfertilization), they were treated with the immunosuppressant chloramphenicol to create an immunosuppressive model, and hyaluronic acid samples were added for treatment. After 24 hours of sample treatment, the samples were observed under a fluorescence microscope, photographed, and the number of immune cells was counted. GraphPad software was used to perform statistical analysis on the data. The results are shown in Table 2.

[0194] Table 2 Effects of hyaluronic acid composition on zebrafish immune cells

[0195] Group Dosage (μg / mL) Number of immune cells Control group 200 55.4±4.06 Model Group 200 <![CDATA[29.4±3.68 ## ]]> Example 1 200 <![CDATA[54.2±6.31 ** ]]> Example 2 200 <![CDATA[49.0±3.09 ** ]]> Example 3 200 <![CDATA[46.4±4.76 ** ]]> Example 4 200 <![CDATA[48.5±1.07 ** ]]> Example 5 200 <![CDATA[47.3±2.68 ** ]]> Example 6 200 <![CDATA[45.2±2.02 ** ]]> Example 7 200 <![CDATA[45.9±5.18 ** ]]> Example 8 200 <![CDATA[44.0±6.00 ** ]]> Example 9 200 <![CDATA[50.2±1.80 ** ]]> Example 10 200 <![CDATA[53.1±3.09 ** ]]> Embodiment 11 200 <![CDATA[51.7±2.24 ** ]]> Comparative Example 1 200 37.6±1.86 Comparative Example 2 200 <![CDATA[40.5±3.07 * ]]> Comparative Example 3 200 <![CDATA[42.0±1.89 * ]]> Comparative Example 4 200 36.2±4.03 Comparative Example 5 200 <![CDATA[41.3±2.97 * ]]> Comparative Example 6 200 32.3±2.11 Comparative Example 7 200 40.9±3.04

[0196] Note: Compared with the control group, #: P<0.05, significant; ##: P<0.01, extremely significant. Compared with the model group, *: P<0.05, significant; **: P<0.01, extremely significant.

[0197] As can be seen from Table 2, compared with the control group, the immune cells in the model group were disordered and the number was significantly reduced, indicating that the model was successfully constructed; compared with the model group, the number of zebrafish immune cells in the sample group increased significantly, especially Example 1 had the best effect, followed by 10, 11, 9, and 2, indicating that feeding the zebrafish with the hyaluronic acid composition can significantly improve its immune activity.

[0198] Experimental Example 2 Experiment on regulating intestinal motility activity

[0199] Wild AB zebrafish of 5dpf (day post fertilization) were used as experimental animals to study the effect of hyaluronic acid samples on intestinal motility. After pre-staining the zebrafish intestine with calcein, the mean fluorescence intensity (IOD) of the intestine was calculated. The sample was then incubated with the pre-stained zebrafish in the dark, and the IOD of the zebrafish intestine after sample treatment was calculated. According to the formula: intestinal IOD coefficient = (intestinal IOD mean before sample treatment - intestinal IOD mean after sample treatment) / intestinal IOD mean before sample treatment, the intestinal IOD coefficient of the zebrafish in the sample treatment group was calculated, and statistical analysis was performed. The results are shown in Table 3.

[0200] Table 3 Experimental study on the effect of hyaluronic acid composition on intestinal peristalsis of zebrafish

[0201] Group Dosage (μg / mL) Intestinal IOD coefficient Control group 200 0.41±0.024 Example 1 200 <![CDATA[0.87±0.015 ** ]]> Example 2 200 <![CDATA[0.77±0.034 ** ]]> Example 3 200 <![CDATA[0.76±0.036 ** ]]> Example 4 200 <![CDATA[0.75±0.021 ** ]]> Example 5 200 <![CDATA[0.70±0.022 * ]]> Example 6 200 <![CDATA[0.65±0.039 * ]]> Example 7 200 <![CDATA[0.70±0.060 * ]]> Example 8 200 <![CDATA[0.62±0.073 * ]]> Example 9 200 <![CDATA[0.80±0.053 ** ]]> Example 10 200 <![CDATA[0.84±0.044 ** ]]> Embodiment 11 200 <![CDATA[0.82±0.046 ** ]]> Comparative Example 1 200 0.52±0.013 Comparative Example 2 200 0.52±0.032 Comparative Example 3 200 0.56±0.017 Comparative Example 4 200 0.49±0.042 Comparative Example 5 200 0.54±0.032 Comparative Example 6 200 0.43±0.016 Comparative Example 7 200 0.53±0.022

[0202] Note: Compared with the control group, *: P<0.05, significant; **: P<0.01, extremely significant.

[0203] It can be seen from Table 3 that compared with the blank control group, the fluorescent dye in the intestine of zebrafish in the sample group was significantly reduced, especially Example 1 had the best effect, followed by 10, 2, 9, and 11, indicating that the hyaluronic acid composition can promote intestinal peristalsis.

[0204] Experiment 3: Promoting the growth of intestinal probiotics

[0205] The intestinal probiotic bacteria Lactobacillus rhamnosus and Lactobacillus helveticus were selected as the starting strains. The conventional Lactobacillus culture medium MRS was used as the basic culture medium. The hyaluronic acid or its salt sample was 0.2% (w / v) of the culture medium volume, the negative control was the sugar-free MRS culture medium, and the positive control was the glucose MRS culture medium with the same concentration of HA. The bacterial concentration (A) at different culture times was detected. 600 ), a total of 8 strains of intestinal probiotics were selected for in vitro proliferation experiments, and the results are shown in Table 4.

[0206] Table 4 Effect of hyaluronic acid composition on probiotic growth (OD 600 )

[0207]

[0208] Note: Compared with the control group, *: P<0.05, significant; **: P<0.01, extremely significant.

[0209] It can be seen from Table 4 that the HA mixture has different degrees of growth-promoting effects on the eight probiotics. Taking the data of 12h as an example, compared with the blank control group, the test samples can effectively increase the concentration and viable count of intestinal probiotics, especially Example 1 has the best effect, followed by 10, 11, 9, and 2, indicating that the hyaluronic acid composition has the function of promoting the growth of intestinal probiotics.

[0210] Experiment 4: Relieve intestinal inflammation

[0211] Fluorescent zebrafish with specifically labeled immune cells at 3dpf were used as experimental animals and randomly divided into blank control group, model group and different sample groups. No drugs or test substances were added to the blank control group. Trinitrobenzene sulfonic acid (TNBS) with a final concentration of 50μg / mL was added to the model group and different sample groups at the same time. There were 10 larvae in each group, and 2 replicates were set at the same time. The zebrafish in the above groups were placed in a constant temperature incubator at 28.5℃ and incubated continuously for 3 days in the dark. After the treatment, the larvae in each group were washed, and the larvae in the blank control group and the model group were placed in new culture water, and the larvae in other groups were placed in culture water containing 100μg / mL different samples to be tested, and cultured for 3 days at 28.5℃. After the treatment, the number of immune cells showing red fluorescence in the intestinal part of zebrafish was counted using Imagepro-plus software, and the data were statistically analyzed using GraphPad software.

[0212] Table 5 Effect of hyaluronic acid composition on gastrointestinal inflammation

[0213] Group Dosage (μg / mL) Immune cell count Control group 100 19.9±0.936 Model Group 100 <![CDATA[28.9±1.441 ## ]]> Example 1 100 <![CDATA[20.3±0.615 * ]]> Example 2 100 <![CDATA[18.5±1.441 ** ]]> Example 3 100 <![CDATA[18.2±1.013 ** ]]> Example 4 100 <![CDATA[17.7±0.775 ** <!-- 14 -->]]> Example 5 100 <![CDATA[19.2±1.114 * ]]> Example 6 100 <![CDATA[18.9±1.174 * ]]> Example 7 100 <![CDATA[19.9±0.900 * ]]> Example 8 100 <![CDATA[20.5±1.062 * ]]> Example 9 100 <![CDATA[19.9±0.632 * ]]> Example 10 100 <![CDATA[17.2±0.791 ** ]]> Embodiment 11 100 <![CDATA[19.2±0.466 * ]]> Comparative Example 1 100 24.7±0.800 Comparative Example 2 100 24.2±1.012 Comparative Example 3 100 23.5±1.061 Comparative Example 4 100 22.7±1.067 Comparative Example 5 100 22.3±1.078 Comparative Example 6 100 25.5±0.614 Comparative Example 7 100 23.4±0.723

[0214] Note: Compared with the control group, ##: P<0.01, extremely significant. Compared with the model group, *: P<0.05, significant; **: P<0.01, extremely significant.

[0215] As can be seen from Table 5, compared with the blank control group, the number of immune cells in the intestinal part of the zebrafish in the model group increased significantly, and it was statistically significant; this result shows that when the zebrafish is in an intestinal inflammatory state, the immune cells in the body will migrate to the inflammatory site. When the zebrafish were treated with different samples, the number of immune cells in the intestinal part decreased compared with the model group, and it was extremely statistically significant; this result shows that the test samples alleviated the inflammatory state of the zebrafish intestine to varying degrees, so the number of immune cells migrating to the zebrafish intestine also decreased.

[0216] In summary, the use of the composition described in the present invention can significantly improve the number of immune cells in zebrafish with immune disorders, enhance immune activity, promote intestinal peristalsis, increase the survival rate of various intestinal probiotics, improve intestinal flora, and significantly relieve enteritis. This shows that the composition described in the present invention has a significant effect on improving immunity and protecting the gastrointestinal tract, and can be added to foods and health foods as an effective ingredient, or used in combination with drugs.

[0217] The above is only a preferred embodiment of the present invention, and does not limit the present invention in other forms. Any technician familiar with the profession may use the above disclosed technical content to change or modify it into an equivalent embodiment with equivalent changes. However, any simple modification, equivalent change and modification made to the above embodiment according to the technical essence of the present invention without departing from the technical solution of the present invention still belongs to the protection scope of the technical solution of the present invention.

Claims

1. A composition containing hyaluronic acid or its salt, comprising three molecular weight hyaluronic acids or their salts, wherein: The molecular weight of the first molecular weight hyaluronic acid or its salt is 20k-160kDa; The molecular weight of the second molecular weight hyaluronic acid or its salt is 280k-600kDa; The molecular weight of the third molecular weight hyaluronic acid or its salt is 1000k-1500kDa; Taking the total number of parts after mixing as 10 parts by weight, The first molecular weight hyaluronic acid or its salt is 1-4 parts; The second molecular weight hyaluronic acid or its salt is 3-6 parts; and The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

2. The composition according to claim 1, wherein The molecular weight of the first molecular weight hyaluronic acid or its salt is 20k-100kDa; The molecular weight of the second molecular weight hyaluronic acid or its salt is 280k-400kDa; The molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa.

3. The composition according to claim 1, wherein The molecular weight of the first molecular weight hyaluronic acid or its salt is 30k-50kDa; The molecular weight of the second molecular weight hyaluronic acid or its salt is 280k-400kDa; The molecular weight of the third molecular weight hyaluronic acid or its salt is 1300k-1500kDa.

4. The composition according to any one of claims 1 to 3, wherein Taking the total number of parts after mixing as 10 parts by weight, The first molecular weight hyaluronic acid or its salt is 2-4 parts; The second molecular weight hyaluronic acid or its salt is 4-6 parts; and The ratio of the third molecular weight hyaluronic acid or its salt is 1-3 parts.

5. The composition according to any one of claims 1 to 3, wherein The hyaluronic acid or its salt is selected from one or more of hyaluronic acid, sodium hyaluronate, potassium hyaluronate, calcium hyaluronate and zinc hyaluronate.

6. The composition according to any one of claims 1 to 3, wherein the salt of hyaluronic acid is sodium hyaluronate or potassium hyaluronate.

7. Use of the composition according to any one of claims 1 to 6 in the field of preparing food or medicine.

8. Use of the composition according to any one of claims 1 to 6 in the field of preparing health care products.

9. A food composition comprising the composition according to any one of claims 1 to 6.

10. The food composition according to claim 9, wherein The food composition is used for improving the body's immune ability, increasing intestinal motility and / or regulating intestinal flora.

11. A health care product composition comprising the composition according to any one of claims 1 to 6.

12. The health care product composition according to claim 11, wherein The health care product composition is used to improve the body's immune ability, increase intestinal motility and / or regulate intestinal flora.

13. A pharmaceutical composition comprising the composition according to any one of claims 1 to 6.

14. The pharmaceutical composition according to claim 13, wherein The pharmaceutical composition is used to improve the body's immune ability, relieve intestinal inflammation, increase intestinal motility and / or regulate intestinal flora.

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