A Chinese medicine formula for treating sows' postpartum lactation deficiency and its preparation method

Abstract

The invention discloses a Chinese medicine formula for treating sows' postpartum milk deficiency and a preparation method thereof. The active substances in the Chinese medicine formula include the following components: stalactite, saltpeter, white gypsum, tongcao, platycodon grandiflorum, motherwort, gypsum, rhubarb, sodium bicarbonate, liquorice, compound probiotics, and compound enzyme preparations. Compared with the prior art, the Chinese medicine formula prepared by the invention adopts multiple Chinese medicines and is divided into two doses, which respectively solve the problem of sows' postpartum milk deficiency and the problem of subsequent defecation difficulties, and has the effects of antibacterial and anti-inflammatory, antiviral, improving body immunity, regulating intestinal flora, and promoting intestinal digestion and absorption.

Description

Technical Field

[0001] The invention relates to the technical field of livestock feed additives, and in particular to a Chinese medicine formula for treating sows' postpartum lactation deficiency and a preparation method thereof. Background Art

[0002] The udder of a sow is quite different from that of other livestock such as cows and sheep. Cows and sheep have milk reservoirs in their udders, but the milk reservoirs in pigs are extremely underdeveloped and cannot store milk, so piglets cannot suck milk at any time. They can only get milk when the sow "let milk". The initiation of this process requires sufficient stimulation of the piglets and increased udder pressure. The stress caused by the external environment to the sow will cause the body to antagonize the release of oxytocin, resulting in stagnation of milk discharge.

[0003] The lactation period of sows is usually 60 days, and the milk production is about 450kg, with an average daily milk production of 7.5kg. The water content of the milk is more than 70%. When the sow loses too much water, it will enter the feedback regulation stage, and the back half of the intestine will strengthen the water reabsorption, resulting in dry stools and difficulty in defecation, which will affect the sow's feed intake and thus affect the sow's continuous lactation. The lactation stimulants currently used in the market only have a certain therapeutic effect on the low milk production of sows after delivery, and there is no relevant treatment for postpartum constipation or dry stools that are likely to be caused in sows. Therefore, it is very important to improve the original lactation stimulant feed additives, expand the indications, and promote sow digestion and absorption.

[0004] Invention patent CN104306472A discloses a Chinese medicine preparation for preventing and treating postpartum hypogalactia in sows and its preparation method, which is composed of the following raw materials by weight: 5-15 parts of roasted Astragalus, 8-13 parts of Ligustrum lucidum, 6-15 parts of Vaccaria segetalis, 3-10 parts of Patrinia scabra, 4-12 parts of Scutellaria baicalensis, 10-30 parts of Dipsacus asper, 4-12 parts of Epimedium, 5-15 parts of Magnolia officinalis, 7-15 parts of Taraxacum officinale, 5-13 parts of Red Peony Root, and 2-7 parts of Angelica sinensis. The Chinese medicine oral powder and injection prepared from the above raw materials for preventing and treating postpartum hypogalactia in sows are safe and reliable, have no side effects and are quick to take effect. They also have preventive and therapeutic effects on sows, can effectively prevent and treat symptoms such as mastitis, endometritis, colds, puerperal fever, etc., can effectively increase lactation by 32%, thereby improving the growth and development level of piglets and increasing the net weight gain of piglets by 10%. However, the Chinese medicine preparation prepared by the invention cannot effectively treat constipation, and the drug absorption is low and the effect is poor. Summary of the invention

[0005] In view of the shortcomings of the Chinese medicine formula in the prior art, such as poor absorption effect, inability to treat constipation and low efficacy, the technical problem to be solved by the present invention is to provide a Chinese medicine formula with good absorption effect and obvious effect for treating sow postpartum low milk production and a preparation method thereof.

[0006] In order to achieve the above-mentioned object of the invention, the present invention adopts the following technical solutions:

[0007] A Chinese medicine formula for treating sows' postpartum lactation deficiency, the preparation method of which is as follows, calculated by weight:

[0008] T1. Add 1 to 3 parts of active ingredients to 8 to 12 parts of water, stir magnetically for 20 to 50 minutes at a stirring speed of 400 to 800 rpm to obtain a drug solution, spray the drug solution on the surface of 0.5 to 2 parts of the modified powder in a fluidized bed, and then dry at 40 to 70°C for 10 to 20 minutes to obtain a drug carrier; the fluidized bed operating parameters are set to a spray rate of 2 to 5 g / min, an atomizing pressure of 1.0 to 3.0 bar, a material temperature of 30 to 40°C, an inlet temperature of 40 to 70°C, and a jet speed of 10 to 30 m 3 / h;

[0009] T2, add 1 to 3 parts of auxiliary agent to 8 to 12 parts of water, then add 0.4 to 0.6 parts of lactose and 0.8 to 1.2 parts of cassava starch and stir for 20 to 40 minutes at a stirring speed of 100 to 400 rpm to prepare a sustained-release coating liquid, and then spray the sustained-release coating liquid on the surface of the drug carrier prepared in step T1 through a fluidized bed. The operating parameters of the fluidized bed are set to a spray rate of 1 to 3 g / min, an atomizing pressure of 1.0 to 3.0 bar, a material temperature of 25 to 35°C, an inlet temperature of 30 to 50°C, and a jet speed of 10 to 20 m 3 / h, and dried at 30-50°C for 20-30h to obtain a traditional Chinese medicine prescription.

[0010] The active ingredients include the following raw materials: stalactite, saltpeter, white ochre, tongcao, platycodon, motherwort, gypsum, rhubarb, sodium bicarbonate, liquorice, compound probiotics and compound enzyme preparation.

[0011] Stalactite has the therapeutic effects of promoting lactation, relieving lower-burner exhaustion, and strengthening yin. It can be supplemented with saltpeter, white litharge, tongcao, platycodon, and licorice to promote lactation in sows with poor milk supply. By feeding gypsum, anemarrhena, processed rhubarb, sodium bicarbonate, licorice, and complex enzyme preparations, the efficacy of traditional Chinese medicine can be improved, and probiotics can be used to treat subsequent intestinal problems, ensuring stable production in the pig farm's delivery room.

[0012] Preferably, the active ingredient comprises the following raw materials in parts by weight: 10-30 parts of stalactite, 10-30 parts of saltpeter, 10-30 parts of white ochre, 20-90 parts of tongcao, 40-80 parts of platycodon, 30-80 parts of motherwort, 30-60 parts of gypsum, 30-60 parts of anemarrhena, 30-80 parts of processed rhubarb, 10-50 parts of sodium bicarbonate, 10-20 parts of licorice, 3-5 parts of compound probiotics, and 1-2 parts of compound enzyme preparation.

[0013] The preparation method of the active ingredient comprises the following steps:

[0014] Step 1, weighing stalactite, saltpeter, white ochre, tongcao, platycodon grandiflorum, and motherwort according to weight; grinding and cooling to room temperature to obtain a Chinese medicine mixture A;

[0015] Step 2, weighing gypsum, rhubarb, processed rhubarb, sodium bicarbonate, and liquorice according to weight, grinding and cooling to room temperature to obtain a traditional Chinese medicine mixture B;

[0016] Step 3: mix the Chinese medicine mixture A prepared in step 1 and the Chinese medicine mixture B prepared in step 2, grind and sieve to obtain a Chinese medicine mixed powder, add probiotic powder, then add complex enzyme preparation powder, stir, dry, and obtain active ingredients.

[0017] Preferably, the method for preparing the active ingredient comprises the following steps:

[0018] Step 1, weigh stalactite, saltpeter, white ochre, tongcao, platycodon grandiflorum, and motherwort according to weight; put them into a grinder, grind for 1 to 3 hours, and then cool them to room temperature to obtain a Chinese medicine mixture A;

[0019] Step 2, weighing gypsum, rhubarb, processed rhubarb, sodium bicarbonate, and liquorice according to weight, putting them into a grinder, grinding them for 1 to 3 hours, and then cooling them to room temperature to obtain a traditional Chinese medicine mixture B;

[0020] Step 3, the Chinese medicine mixture A prepared in step 1 and the Chinese medicine mixture B prepared in step 2 are mixed, ground for 2 to 5 hours, and then sieved through a 60-100 mesh sieve to obtain a Chinese medicine mixed powder, probiotic powder is added, and then the composite enzyme preparation powder is added, stirred for 20 to 40 minutes at a stirring speed of 50 to 200 rpm, and then dried at 30 to 50° C. to control the water content to 5 to 10 wt%, to obtain an active ingredient.

[0021] The probiotic powder is a freeze-dried powder, which is composed of the following strains in parts by weight: 1-2 parts of Lactobacillus plantarum, 2-3 parts of Bacillus subtilis, 1-2 parts of Saccharomyces cerevisiae, 1-2 parts of Enterococcus faecalis, and 1-2 parts of Bacillus coagulans.

[0022] The composite enzyme preparation powder is a dry powder, which is composed of the following enzyme preparations in parts by weight: 3 to 5 parts of cellulase, 2 to 3 parts of xylanase, and 2 to 3 parts of glucose oxidase.

[0023] The preparation method of the auxiliary agent is as follows:

[0024] S1. Crush the shrimp and crab shells, sieve them, add them to an aqueous sodium hydroxide solution, stir, add anhydrous ethanol, stir, filter, collect the solids, wash, and dry them to obtain a pretreated product;

[0025] S2, adding the pretreated product prepared in step S1 to an aqueous sodium hydroxide solution, stirring, cooling in an ice-water bath, sieving, collecting solids, and rinsing to obtain an extract;

[0026] S3, adding L-histidine to water to obtain an L-histidine aqueous solution, adding sodium alginate powder to water, stirring to obtain a homogeneous solution; adding the extract prepared in step S2 to the homogeneous solution, then adding glycerol, stirring, adding the L-histidine aqueous solution, and stirring again,

[0027] After evaporation, the solution is placed in an incubator to obtain an auxiliary agent.

[0028] Preferably, the preparation method of the auxiliary agent is as follows, in parts by weight:

[0029] S1. Use an ultrafine grinder to crush 3-5 parts of shrimp and crab shells into fine particles, pass through a 100-300 mesh sieve, add to 15-25 parts of 2-6wt% sodium hydroxide aqueous solution, stir at 60-70°C for 3-8h, stirring at a speed of 100-300rpm, add 4-6 parts of anhydrous ethanol, stir at room temperature for 4-8h, stirring at a speed of 200-600rpm, then filter through a 300-500 mesh sieve, collect solids, wash with water until the washing water is neutral, and dry in a vacuum oven at 40-70°C for 10-20h to obtain a pretreated product;

[0030] S2, adding 0.5-1.5 parts of the pretreated product prepared in step S1 to 2-3 parts of 30-35wt% sodium hydroxide aqueous solution, stirring at 70-110°C for 1-5h, stirring at a speed of 100-300rpm, cooling to room temperature, cooling in an ice water bath, passing through a 300-500 mesh sieve, collecting the solid, and washing with water 1-3 times to obtain an extract;

[0031] S3, adding 0.4-0.8 parts of L-histidine to 2-5 parts of water to obtain an L-histidine aqueous solution, adding 0.4-0.8 parts of sodium alginate powder to 4-6 parts of water at 20-30° C., stirring for 1-3 hours to obtain a homogeneous solution; adding 0.1-0.5 parts of the extract prepared in step S2 to the homogeneous solution, then adding 0.04-0.08 parts of glycerol, stirring at 300-600 rpm for 1-3 hours, adding the L-histidine aqueous solution, stirring for another 10-30 minutes, the stirring speed is 100-300 rpm, evaporating at 40-60° C. for 60-80 hours, and then placing in an incubator at a temperature of 20-30° C. and a relative humidity of 45-55% for 30-50 hours to obtain an auxiliary agent.

[0032] The preparation method of the modified powder is as follows:

[0033] Z1, konjac mannan is added to the water, stirred, and with sodium hydroxide aqueous solution, pH is adjusted, after adding hydrogen peroxide, sodium bisulfate aqueous solution is added to terminate the reaction, and pH is adjusted, and then anhydrous ethanol precipitation is used, filtered, cleaned, dried, ground and sieved, and pre-treated powder is obtained;

[0034] Z2. Add the pretreated powder prepared in step Z1 into water, then slowly add cassava starch, stir, dry, grind, sieve, and then heat to obtain modified powder.

[0035] Preferably, the preparation method of the modified powder is as follows, in parts by weight:

[0036] Z1, 1.5~2 parts of konjac mannan are joined in 8~12 parts of water, at 400~600rpm and 40~50 ℃ of stirring 20~40min, with 0.5~2wt% sodium hydroxide aqueous solution, pH is adjusted to 7.5~8.5, add 0.2~0.5 part of hydrogen peroxide every 5~15min, add 2~5 times, after 3~5h, add 0.5~2mol / L sodium bisulfate aqueous solution, pH is adjusted to 6~7, use the absolute ethanol precipitation then, and filter by vacuum filter, clean 2~5 times with 65~80wt% ethanol aqueous solution, at 40~60 ℃ of vacuum drying 1~3h, grind through 60~150 mesh sieve, obtain pre-treatment powder;

[0037] Z2. Add 0.5-2 parts of the pretreated powder prepared in step Z1 to 3-5 parts of water, then slowly add 0.1-0.3 parts of cassava starch, stir at room temperature for 0.5-2h, stirring speed is 100-300rpm, dry at 30-50°C to a moisture content of 6-12wt%, further grind, pass through a 60-200 mesh sieve, and then heat at 110-140°C for 2-6h to obtain a modified powder.

[0038] Stalactite, sweet, warm, non-toxic; enters the spleen meridian of foot Taiyin, lung meridian of hand Taiyin, kidney meridian of foot Shaoyin, liver meridian of foot Jueyin. Functions and indications: five kinds of labor and seven kinds of injuries, cough and reverse breathing, treat cold cough, clear the voice, soothe the five internal organs, cure all kinds of diseases, benefit the nine thefts, promote lactation, replenish qi and supplement deficiency, treat weak pain and cold, lower burner injury and exhaustion, and strengthen yin.

[0039] Nitrum, also known as saltpeter, potassium saltpeter, etc.; bitter, cold, non-toxic. Functions and indications: heat accumulation in the five internal organs, stomach bloating and closure, cleansing the accumulated food, removing the old and bringing in the new, and removing evil spirits. Sudden cold, severe fever in the abdomen, stopping irritability and thirst, promoting urination, and treating ulcers. It can break up accumulation and disperse hardness, treat abdominal bloating, break blood, and expel scrofula, and eliminate the root cause.

[0040] Halloysitum Album, sweet, sour, flat, non-toxic. Functions and indications: It is an astringent and antidiarrheal medicine. It nourishes the lung qi, thickens the intestines, replenishes the bone marrow, treats the deficiency of the five internal organs, palpitations, heart trouble, stops abdominal pain, diarrhea, small intestine turmoil, hot loose stools with pus and blood, women's metrorrhagia, discharges carbuncle, sore and hemorrhoids. It is now generally used as an absorbent.

[0041] Tongcao, Tetrapanax papyrifer (Hook.) K.Koch, also known as Kou Tuo, Linan, etc.; sweet; light; slightly cold; non-toxic. Enter the lungs, large and small intestines. Functions: improve eyesight, reduce fever, induce labor, promote the release of the uterus, and promote lactation.

[0042] Platycodon grandiflorus (Jacq.) A.DC, pungent, slightly warm, crude platycodon saponin has sedative, analgesic and antipyretic effects. Functions and indications: clearing the lungs, relieving sore throat, expectorant, pus discharge. Used for cough with a lot of sputum, chest tightness, sores and ulcers with pus that do not break.

[0043] Motherwort, Leonurus species, tastes slightly bitter and cold in nature. It has the effects of promoting blood circulation and removing blood stasis, reducing inflammation, clearing away heat and detoxifying. Its active ingredient is leonurine.

[0044] Gypsum Fibrosum, sweet and spicy, very cold in nature. Enters the lung and stomach meridians. Functions and indications: clearing away heat and purging fire, relieving restlessness and quenching thirst; calcined: astringing sores, promoting tissue regeneration, absorbing dampness, and stopping bleeding.

[0045] Anemarrhena asphodeloides Bunge, bitter, cold, enter the lung, stomach and kidney meridians. Functions and indications: thirst due to febrile disease, dry cough due to lung heat, hot flashes due to bone steaming, internal heat and thirst, and constipation due to dry intestines.

[0046] Prepared rhubarb, Rheum officinale Baill, functions and indications: purgative and attacking accumulation, clearing away heat and purging fire, cooling blood and detoxifying, removing blood stasis and dredging menstruation.

[0047] Sodium bicarbonate is odorless, alkaline in taste, easily soluble in water, and can make up for the lack of lysine in feed.

[0048] The feed additive of a lactation promoter containing traditional Chinese medicine and probiotics and the preparation method thereof are prepared according to common problems of sows after delivery by adopting a traditional Chinese medicine formula, wherein the traditional Chinese medicine group: stalactite and tongcao have the functions of promoting lactation, saltpeter, motherwort, platycodon grandiflorum and white ochre have the functions of anti-inflammatory, antiviral, clearing away heat and detoxifying and promoting the discharge of lochia, gypsum, rhubarb, processed rhubarb and sodium bicarbonate have the functions of removing stomach fire, cooling blood and alleviating defecation difficulty, and liquorice is used to neutralize the cooling property of gypsum, rhubarb and processed rhubarb.

[0049] The effects of each strain of probiotics are as follows: Lactobacillus can secrete vitamins and also has a therapeutic effect on constipation; yeast has the effect of promoting digestion; Bacillus can colonize in the intestines, promote the balance of intestinal flora, and assist in nutrient absorption; Enterococcus faecalis can secrete natural antibiotics and probiotic substances, which can inhibit the growth of Escherichia coli and Salmonella in the intestines; Bacillus coagulans can colonize in the latter half of the intestine, and at the same time secrete amino acids, vitamins and a variety of digestive enzymes, which are beneficial to the health of the body.

[0050] The functions of various enzymes in enzyme preparations are as follows: cellulase can convert insoluble cellulose into glucose and destroy the cell walls of Chinese medicinal plants, thereby increasing the release of medicinal ingredients in Chinese medicinal plant cells, increasing the content and absorption of medicinal ingredients, and thus improving the efficacy of Chinese medicine; xylanase mainly degrades heterogeneous polysaccharides in the cell walls of Chinese medicinal plants and degrades hemicellulose, thereby releasing the medicinal ingredients in the cell walls of Chinese medicinal plants; glucose oxidase can regulate the glucose content in blood sugar, thereby increasing the animal's feed intake and increasing the energy source for lactation.

[0051] The present invention uses sodium hydroxide to adjust konjac mannan, adds hydrogen peroxide to react, uses sodium bisulfate to terminate the reaction, mixes cassava starch, and obtains a modified powder after dry heat treatment. The solubility and swelling power are low, which is conducive to loading active ingredients. In addition, the addition of cassava starch significantly reduces the porosity and swelling rate of the modified powder, delays the release of the drug, and has good sustained-release performance. Then, the extract prepared from shrimp and crab shells is used as a cross-linking agent for sodium alginate powder, and the auxiliary agent is successfully prepared. Sodium alginate powder and L-histidine exist in calcium ion cross-linking, polyelectrolyte interaction and hydrogel bonding interaction with glycerol and extract, and then mixed with lactose and starch to improve the thermal stability of the outer coating film, improve the strength, mechanical properties and thermal stability of the auxiliary agent-coated drug carrier, and can show the ability of sustained release of drugs. In addition, the Chinese medicine group prepared by the spraying process has good squareness, good fluidity and good stability, can slowly release drugs in sow intestinal fluid, and improves the oral bioavailability of sows.

[0052] Compared with the prior art, the present invention has the following beneficial effects:

[0053] 1) The Chinese medicine formula prepared by the present invention adopts multiple Chinese medicines and is divided into two doses, which respectively solve the problem of sows' low milk production after delivery and the problem of subsequent defecation difficulties, and has the effects of antibacterial and anti-inflammatory, antiviral, improving body immunity, regulating intestinal flora, and promoting intestinal digestion and absorption;

[0054] 2) By preparing modified powders and additives, the strength, mechanical properties and thermal stability of the coated drug carrier are improved. The Chinese medicine group prepared by the spraying process has good roundness, good fluidity and good stability, can slowly release the drug in the intestinal fluid of sows, shows the ability of sustained drug release, and improves the oral bioavailability of sows. DETAILED DESCRIPTION

[0055] Main sources of substances:

[0056] Saltpeter: Originated from Hotan, Xinjiang.

[0057] Tongcao: Originated from Yulin, Shaanxi.

[0058] Platycodon: Origin: Yingshan, Hubei.

[0059] Anemarrhena asphodeloides: Origin: Minxian County, Gansu Province.

[0060] Motherwort: Origin: Jianou City, Fujian Province.

[0061] White ochre: Origin: Dongyang City, Zhejiang Province.

[0062] Gypsum: Origin: Zaozhuang, Shandong. Kuandian Manchu Autonomous County, Liaoning Province.

[0063] Stalactite: Origin: Dujiangyan, Sichuan.

[0064] Processed rhubarb: Origin: Longxi, Gansu.

[0065] Licorice: Originated from Shuozhou, Shanxi.

[0066] Shrimp and crab shells, Linqu County Huamao Feed Co., Ltd., item number: XKF0261.

[0067] Konjac mannan: Xi'an Four Seasons Biotechnology Co., Ltd., product number: SJMYPGLJT.

[0068] Lactobacillus plantarum: Latin name: Lactobacillus plantarum, strain number: CICC22209.

[0069] Bacillus subtilis: Latin name: Bacillus subtilis, strain number: CICC 25064.

[0070] Saccharomyces cerevisiae: Latin name: Saccharomyces cerevisiae strain number: CICC32883.

[0071] Enterococcus faecalis: Latin name: Enterococcus faecalis, strain number: CICC20396.

[0072] Bacillus coagulans, Latin name: Bacillus coagulans, strain number: ATCC7050 (American Type Culture Collection).

[0073] Cellulase, Jiangsu Yanke Biological Co., Ltd., product number: 1011, enzyme activity: 100,000;

[0074] Xylanase, Guangzhou Huaxi Biotechnology Co., Ltd., product number: 101, enzyme activity: 10,000;

[0075] Glucose oxidase, Novozymes China Investment Co., Ltd., model: Gluzyme Mono10000B, enzyme activity: 10000GODU / g;

[0076] Example 1

[0077] A Chinese medicine formula for treating sows' postpartum lactation deficiency, the preparation method of which is as follows:

[0078] T1. Add 2 kg of active ingredient into 10 kg of water, stir magnetically for 30 min at a stirring speed of 600 rpm to obtain a drug solution, spray the drug solution on the surface of 1 kg of modified powder in a fluidized bed, and then dry at 60 ° C for 15 min to obtain a drug carrier; the fluidized bed operating parameters are set to a spray rate of 4 g / min, atomization pressure of 2.0 bar, material temperature of 32 ° C, inlet temperature of 50 ° C, and a jet speed of 19 m 3 / h;

[0079] T2, add 1.5kg of auxiliary agent to 10kg of water, then add 0.5kg of lactose and 1kg of cassava starch and stir for 30min at a stirring speed of 200rpm to prepare a sustained-release coating liquid, and then spray the sustained-release coating liquid on the surface of the drug carrier prepared in step T1 through a fluidized bed. The fluidized bed operation parameters are set to a spray rate of 2g / min, an atomizing pressure of 1.44bar, a material temperature of 27.5°C, an inlet temperature of 35°C, and a jet speed of 15.5m 3 / h, and dried at 40°C for 24h to obtain the Chinese medicine formula.

[0080] The preparation method of the active ingredient comprises the following steps:

[0081] Step 1, weigh 2kg of stalactite, 2kg of saltpeter, 2kg of white ochre, 5kg of tongcao, 6kg of platycodon grandiflorum, and 5kg of motherwort; put them into a grinder and grind for 2h to obtain a Chinese medicine mixture A;

[0082] Step 2, weigh 4 kg of gypsum, 3 kg of rhubarb, 5 kg of processed rhubarb, 3 kg of sodium bicarbonate, and 1.5 kg of liquorice, put them into a grinder and grind them for 2 hours to obtain a Chinese medicine mixture B;

[0083] Step 3, the Chinese medicine mixture A prepared in step 1 and the Chinese medicine mixture B prepared in step 2 are mixed, ground for 3 hours, and then passed through an 80-mesh sieve to obtain a Chinese medicine mixed powder, 0.4 kg of probiotic powder is added, and then 0.18 kg of complex enzyme preparation powder is added, stirred for 30 minutes at a stirring speed of 100 rpm, and then dried at 40°C to control the water content to 7wt%, to obtain an active ingredient.

[0084] The probiotic powder is a freeze-dried powder, which is composed of the following strains: 0.075 kg of Lactobacillus plantarum, 0.1 kg of Bacillus subtilis, 0.075 kg of Saccharomyces cerevisiae, 0.075 kg of Enterococcus faecalis, and 0.075 kg of Bacillus coagulans.

[0085] The composite enzyme preparation powder is a dry powder, which is composed of the following enzyme preparations: 0.08 kg of cellulase, 0.05 kg of xylanase, and 0.05 kg of glucose oxidase.

[0086] The preparation method of the auxiliary agent is as follows:

[0087] S1. Use an ultrafine grinder to crush 4 kg of shrimp and crab shells into fine particles, pass through a 200-mesh sieve, add to 20 kg of a 5 wt% sodium hydroxide aqueous solution, stir at 65 ° C for 5 h, and stir at a speed of 200 rpm. Add 5 kg of anhydrous ethanol, stir at room temperature for 6 h, and stir at a speed of 400 rpm. Then filter through a 400-mesh sieve, collect the solid, wash with water until the washing water is neutral, and dry in a vacuum oven at 60 ° C for 12 h to obtain a pretreated product;

[0088] S2, adding 1 kg of the pretreated product prepared in step S1 to 2.5 kg of a 33 wt% sodium hydroxide aqueous solution, stirring at 100° C. for 3 h at a stirring speed of 200 rpm, cooling to room temperature, cooling in an ice water bath, passing through a 400 mesh sieve, collecting the solid, and rinsing with water twice to obtain an extract;

[0089] S3, adding 0.6 kg of L-histidine to 3 kg of water to obtain an L-histidine aqueous solution; at 25° C., adding 0.6 kg of sodium alginate powder to 5 kg of water, stirring for 2 hours to obtain a homogeneous solution; adding 0.3 kg of the extract prepared in step S2 to the homogeneous solution, then adding 0.06 kg of glycerol, stirring at 500 rpm for 2 hours, adding the L-histidine aqueous solution, stirring for another 20 minutes, the stirring speed is 200 rpm, evaporating at 45° C. for 72 hours, and then placing in an incubator at a temperature of 25° C. and a relative humidity of 50% for 48 hours to obtain an adjuvant.

[0090] The preparation method of the modified powder is as follows:

[0091] Z1, 1.8kg konjac mannan is joined in 10kg water, at 45 ℃, stir 30min with 500rpm, with 1wt% sodium hydroxide aqueous solution, regulate pH to 8.0, add 0.4kg hydrogen peroxide every 10min, add 3 times, after 4h, add 1mol / L sodium bisulfate aqueous solution and regulate pH to 6.5, then use the absolute ethanol precipitation, and filter by vacuum filter, clean 3 times with 70wt% ethanol aqueous solution, at 50 ℃ of vacuum drying 2h, grind by 100 mesh sieves, obtain pre-treatment powder;

[0092] Z2. Add 1 kg of the pretreated powder prepared in step Z1 to 4 kg of water, then slowly add 0.2 kg of cassava starch, stir at room temperature for 1 hour, the stirring speed is 200 rpm, dry at 45 ° C to a moisture content of 10 wt%, further grind, pass through a 100 mesh sieve, and then heat at 130 ° C for 4 hours to obtain a modified powder.

[0093] Example 2

[0094] The preparation method of a traditional Chinese medicine formula for treating sows' postpartum insufficient milk production is substantially the same as that of Example 1, with the only difference being that the preparation methods of the traditional Chinese medicine formula are different.

[0095] A preparation method of a traditional Chinese medicine formula for treating sow postpartum lactation is as follows: 1.5 kg of an auxiliary agent is added to 10 kg of water, and then 0.5 kg of lactose and 1 kg of cassava starch are added and stirred for 30 minutes at a stirring speed of 200 rpm to prepare a sustained-release coating liquid, and then the sustained-release coating liquid is sprayed on the surface of 2 kg of active ingredients through a fluidized bed, and the fluidized bed operation parameters are set to a spray speed of 2 g / min, an atomization pressure of 1.44 bar, a material temperature of 27.5°C, an inlet temperature of 35°C, and a spray speed of 15.5 m / s. 3 / h, and dried at 40°C for 24h to obtain the Chinese medicine formula.

[0096] The preparation method of the active ingredient is the same as that in Example 1.

[0097] The preparation method of the auxiliary agent is the same as that of Example 1.

[0098] Example 3

[0099] The preparation method of a traditional Chinese medicine formula for treating sows' postpartum insufficient milk production is substantially the same as that of Example 1, with the only difference being that the preparation methods of the traditional Chinese medicine formula are different.

[0100] A preparation method of a Chinese medicine formula for treating sow postpartum lactation is as follows:

[0101] T1. Add 2 kg of active ingredient into 10 kg of water, stir magnetically for 30 min at a stirring speed of 600 rpm to obtain a drug solution, spray the drug solution on the surface of 1 kg of modified powder in a fluidized bed, and then dry at 60 ° C for 15 min to obtain a drug carrier; the fluidized bed operating parameters are set to a spray rate of 4 g / min, atomization pressure of 2.0 bar, material temperature of 32 ° C, inlet temperature of 50 ° C, and a jet speed of 19 m 3 / h;

[0102] T2, 0.5kg lactose and 1kg cassava starch were added to 10kg water, and then stirred for 30min at a stirring speed of 200rpm to prepare a sustained-release coating liquid, and then the sustained-release coating liquid was sprayed on the surface of the drug carrier prepared in step T1 through a fluidized bed. The fluidized bed operation parameters were set to a spray rate of 2g / min, an atomization pressure of 1.44bar, a material temperature of 27.5°C, an inlet temperature of 35°C, and a jet speed of 15.5m 3 / h, and dried at 40°C for 24h to obtain the Chinese medicine formula.

[0103] The preparation method of the active ingredient is the same as that in Example 1.

[0104] The preparation method of the modified powder is the same as that of Example 1.

[0105] Example 4

[0106] The preparation method of a traditional Chinese medicine formula for treating sows' postpartum insufficient milk production is substantially the same as that of Example 1, with the only difference being that the preparation methods of the traditional Chinese medicine formula are different.

[0107] A preparation method of a Chinese medicine formula for treating sow postpartum lactation is as follows:

[0108] T1. Add 2 kg of active ingredient into 10 kg of water, stir magnetically for 30 min at a stirring speed of 600 rpm to obtain a drug solution, spray the drug solution on the surface of 1 kg of modified powder in a fluidized bed, and then dry at 60 ° C for 15 min to obtain a drug carrier; the fluidized bed operating parameters are set to a spray rate of 4 g / min, atomization pressure of 2.0 bar, material temperature of 32 ° C, inlet temperature of 50 ° C, and a jet speed of 19 m 3 / h;

[0109] T2, add 1.5kg of auxiliary agent to 10kg of water, stir for 30min, and stir at a speed of 200rpm to prepare a sustained-release coating liquid, and then spray the sustained-release coating liquid on the surface of the drug carrier prepared in step T1 through a fluidized bed. The fluidized bed operation parameters are set to a spray rate of 2g / min, an atomizing pressure of 1.44bar, a material temperature of 27.5℃, an inlet temperature of 35℃, and a spray speed of 15.5m 3 / h, and dried at 40°C for 24h to obtain the Chinese medicine formula.

[0110] The preparation method of the active ingredient is the same as that in Example 1.

[0111] The preparation method of the modified powder is the same as that of Example 1.

[0112] The preparation method of the auxiliary agent is the same as that of Example 1.

[0113] Comparative Example 1

[0114] The preparation method of a traditional Chinese medicine formula for treating sows' postpartum low milk production is substantially the same as that in Example 1, with the only difference being that the traditional Chinese medicine formula is replaced with an active ingredient.

[0115] The preparation method of the active ingredient is the same as that in Example 1.

[0116] Test Example 1

[0117] Milk composition testing

[0118] The test site is Huafu Pig Farm in Jinggangshan, Jiangxi Province. All sows are dual-purpose sows, and they are pregnant for the second time. The clinical effect of the Chinese medicine formula for treating sows with low milk after parturition is tested by setting a blank control for normal postpartum sows, and a medication group for poor milk sows and poor milk sows. The medication group for poor milk sows is divided into 5 groups, and the Chinese medicine formula prepared by the embodiment of the present invention and the comparative example is used for feeding. The feeding amount is 1% of the daily feed mass, and each group has 20 heads. The colostrum components, normal milk components, lactation cycle and lactation amount are tested, and the unit is g / 100g, and the average value is taken. The test statistical results are shown in Table 1, Table 2 and Table 3.

[0119] Table 1. Colostrum composition test results

[0120]

[0121] Table 2. Test results of regular milk composition table

[0122]

[0123]

[0124] Table 3. Lactation cycle and milk production test results

[0125]

[0126] Test Example 2

[0127] Determination of defecation difficulty rate in sows

[0128] By statistically analyzing the rate of difficult defecation during the lactation cycle of sows in Test Example 1, the number of sows with constipation and the number of sows with difficult defecation were recorded, and the test results are shown in Table 4.

[0129] Table 4: Test results of sow defecation difficulty rate

[0130]

[0131]

[0132] From Tables 1 to 4, it can be seen that Example 1 has the best effect in treating sows with postpartum lactation, and has a good effect in improving constipation. The main reason may be that in the present invention, stalactites and tongcao have the function of promoting lactation, saltpeter, motherwort, platycodon grandiflorum, and white calcite have the functions of anti-inflammatory, antiviral, clearing away heat and detoxification, and promoting the discharge of lochia, gypsum, rhubarb, processed rhubarb, and sodium bicarbonate have the functions of removing stomach fire, cooling blood, and relieving defecation difficulties, and licorice is used to neutralize the coolness of gypsum, rhubarb, and processed rhubarb. Among probiotics, lactic acid bacteria can secrete vitamins and have a therapeutic effect on constipation; brewer's yeast has the effect of promoting digestion; bacillus can colonize in the intestine, promote the balance of intestinal flora, and help nutrient absorption; enterococcus faecalis can secrete natural antibiotics and beneficial bacteria substances, which can inhibit the growth of Escherichia coli and Salmonella in the intestine; Bacillus coagulans can colonize in the second half of the intestine, and at the same time secrete amino acids, vitamins and a variety of digestive enzymes, which are beneficial to the health of the body. In addition, cellulase can convert insoluble cellulose into glucose and destroy the cell walls of Chinese medicinal plants, thereby increasing the release of medicinal ingredients in Chinese medicinal plant cells, increasing the content and absorption of medicinal ingredients, and thus improving the efficacy of Chinese medicine; xylanase mainly degrades heterogeneous polysaccharides in the cell walls of Chinese medicinal plants and degrades hemicellulose, thereby releasing the medicinal ingredients in the cell walls of Chinese medicinal plants; glucose oxidase can regulate the glucose content in blood sugar, thereby increasing the animal's feed intake and increasing the energy source for lactation.

[0133] Further, the present invention adjusts konjac mannan with sodium hydroxide, adds hydrogen peroxide to react, terminates the reaction with sodium bisulfate, and mixes cassava starch, obtains a modified powder after dry heat treatment, and the solubility and swelling power are low, which is conducive to loading active ingredients. And the incorporation of cassava starch significantly reduces the porosity and swelling rate of the modified powder, delays the release of the drug, and has good sustained release performance. Then, the extract prepared from shrimp and crab shells is used as a cross-linking agent for sodium alginate powder, and an auxiliary agent is successfully prepared. There are calcium ion cross-linking, polyelectrolyte interaction and hydrogel bonding interaction in sodium alginate powder and L-histidine and glycerol and extract, and then the mixing with lactose and starch can improve the thermal stability of the outer coating film, improve the strength, mechanical properties and thermal stability of the auxiliary agent-coated drug carrier, and can show the ability to continuously release drugs. In addition, the Chinese medicine group prepared by the spraying process has good roundness, good fluidity and good stability, can slowly release drugs in the intestinal fluid of sows, and improves the oral bioavailability of sows.

Claims

1. An active ingredient, characterized in that The invention is composed of the following raw materials in parts by weight: 10-30 parts of stalactite, 10-30 parts of saltpeter, 10-30 parts of white ochre, 20-90 parts of tongcao, 40-80 parts of platycodon, 30-80 parts of motherwort, 30-60 parts of gypsum, 30-60 parts of rhubarb, 30-80 parts of processed rhubarb, 10-50 parts of sodium bicarbonate, 10-20 parts of liquorice, 3-5 parts of compound probiotics, and 1-2 parts of compound enzyme preparation; The preparation method of the active ingredient comprises the following steps: Step 1, weigh stalactite, saltpeter, white stone fat, tongcao, platycodon grandiflorum, and motherwort according to weight; put them into a grinder, grind for 1 to 3 hours, and then cool them to room temperature to obtain a Chinese medicine mixture A; Step 2, weighing gypsum, rhubarb, processed rhubarb, sodium bicarbonate, and liquorice according to weight, putting them into a grinder, grinding them for 1 to 3 hours, and then cooling them to room temperature to obtain a Chinese medicine mixture B; Step 3, the Chinese medicine mixture A prepared in step 1 and the Chinese medicine mixture B prepared in step 2 are mixed, ground for 2-5 hours, and then sieved through a 60-100 mesh sieve to obtain a Chinese medicine mixed powder, probiotic powder is added, and then the composite enzyme preparation powder is added, stirred for 20-40 minutes at a stirring speed of 50-200 rpm, and then dried at 30-50° C. to control the water content to 5-10 wt %, to obtain an active ingredient; The probiotic powder is a freeze-dried powder, which is composed of the following strains in parts by weight: 1-2 parts of Lactobacillus plantarum, 2-3 parts of Bacillus subtilis, 1-2 parts of Saccharomyces cerevisiae, 1-2 parts of Enterococcus faecalis, and 1-2 parts of Bacillus coagulans; The composite enzyme preparation powder is a dry powder, which is composed of the following enzyme preparations in parts by weight: 3-5 parts of cellulase, 2-3 parts of xylanase, and 2-3 parts of glucose oxidase.

2. A method for preparing a Chinese medicine composition for treating sow postpartum lactation, characterized in that: By weight: T1. Add 1 to 3 parts of the active ingredient described in claim 1 to 8 to 12 parts of water, stir magnetically for 20 to 50 minutes at a stirring speed of 400 to 800 rpm to obtain a drug solution, spray the drug solution on the surface of 0.5 to 2 parts of the modified powder in a fluidized bed, and then dry at 40 to 70°C for 10 to 20 minutes to obtain a drug carrier; the fluidized bed operating parameters are set to a spray rate of 2 to 5 g / min, an atomizing pressure of 1.0 to 3.0 bar, a material temperature of 30 to 40°C, an inlet temperature of 40 to 70°C, and a jet speed of 10 to 30 m 3 / h; T2, add 1-3 parts of auxiliary agent to 8-12 parts of water, then add 0.4-0.6 parts of lactose and 0.8-1.2 parts of cassava starch and stir for 20-40 minutes at a stirring speed of 100-400 rpm to prepare a sustained-release coating liquid, and then spray the sustained-release coating liquid on the surface of the drug carrier prepared in step T1 through a fluidized bed. The fluidized bed operation parameters are set to a spray rate of 1-3 g / min, an atomization pressure of 1.0-3.0 bar, a material temperature of 25-35°C, an inlet temperature of 30-50°C, and a jet speed of 10-20 m 3 / h, drying at 30-50°C for 20-30h to obtain a Chinese medicine composition; The preparation method of the auxiliary agent is as follows: S1. Crush the shrimp and crab shells, sieve them, add them to an aqueous sodium hydroxide solution, stir, add anhydrous ethanol, stir, filter, collect the solids, wash, and dry them to obtain a pretreated product; S2, adding the pretreated product prepared in step S1 to an aqueous sodium hydroxide solution, stirring, cooling in an ice-water bath, sieving, collecting solids, and rinsing to obtain an extract; S3, adding L-histidine to water to obtain an L-histidine aqueous solution, adding sodium alginate powder to water, stirring to obtain a homogeneous solution; adding the extract prepared in step S2 to the homogeneous solution, then adding glycerol, stirring, adding the L-histidine aqueous solution, stirring again, evaporating, and then storing in an incubator to obtain an adjuvant; The preparation method of the modified powder is as follows: Z1, konjac mannan is added to the water, stirred, and with sodium hydroxide aqueous solution, pH is adjusted, after adding hydrogen peroxide, sodium bisulfate aqueous solution is added to adjust pH, then with absolute ethanol precipitation, filter, clean, dry, grind and sieve, obtain pre-treatment powder; Z2. Add the pretreated powder prepared in step Z1 into water, then slowly add cassava starch, stir, dry, grind, sieve, and then heat to obtain modified powder.

3. A Chinese medicine composition for treating sows' postpartum lactation deficiency, characterized in that: The method as claimed in claim 2 is used for preparation.

Images