Application of vanillin in inducing the synthesis of benzoxazine compounds

Abstract

The present invention relates to the field of crop technology, and in particular to the use of vanillin in inducing the synthesis of benzoxazine substances. The present invention finds that vanillin can be used to induce plants to produce benzoxazine defense substances, enhance crop resistance, and resist stress. The present invention is to explore the allelopathic mechanism of interspecific interaction inducing plant resistance improvement, and provides a theoretical basis for the rational use of crop diversity planting. At the same time, the above discovery of vanillin can provide materials for the development of new biogenic inducers, which is of great value in reducing the use of other chemical pesticides.

Description

Technical Field

[0001] The invention relates to the technical field of crops, and in particular to application of vanillin in inducing the synthesis of benzoxazine substances. Background Art

[0002] Benzoxazinoids (BXs) are a class of broad-spectrum resistance defense substances widely found in Gramineae plants, with multiple effects such as insect resistance, disease resistance, metal chelation and allelopathic effects. Among them, DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-4-one) and MBOA (6-methoxybenzoxazolin-2-one) are the most widely studied benzoxazinoid defense substances, which can resist biotic and abiotic stresses. Studies have shown that these two compounds have significant inhibitory effects on the release, movement, and germination of zoospores of pepper, Phytophthora fusca and soybean. For example, after intercropping corn and pepper, pepper root secretions can induce the synthesis and secretion of BXs in corn, enhance corn's resistance to its own disease, leaf spot disease, and inhibit the spread of pepper in the field. MBOA, as a direct product of DIMBOA degradation, can affect the defense capabilities of the next generation of plants by regulating the structure of related microbial communities in the soil.

[0003] Intercropping corn with other plants has become an important production method to ensure food production. For example, intercropping corn with soybeans, peppers, potatoes, tobacco, sugarcane and other species can effectively increase yields and reduce the prevalence of diseases. Intercropping can improve the utilization efficiency of resources such as light, temperature, water, and fertilizer, induce the synthesis of benzoxazine substances, and increase crop resistance, but little is known about the signal transmission and response strategies of resistance induced by interspecific interactions in intercropped plants.

[0004] Therefore, there is an urgent need to find some signal compounds that induce the production of the resistance substance MBOA to provide technical support for the utilization of corn stress resistance resources. Summary of the invention

[0005] In a first aspect, the present invention provides use of vanillin or a pharmaceutically acceptable salt, hydrate, or solvate thereof in inducing the synthesis of benzoxazine substances.

[0006] Preferably, the benzoxazine substance is MBOA.

[0007] In a second aspect, the present invention provides use of vanillin or a pharmaceutically acceptable salt, hydrate, or solvate thereof in preventing and controlling crop diseases.

[0008] Preferably, the disease is corn leaf blight.

[0009] In a third aspect, the present invention provides use of vanillin or a pharmaceutically acceptable salt, hydrate, or solvate thereof in inducing or enhancing crop resistance.

[0010] In a fourth aspect, the present invention provides the use of vanillin or its pharmaceutically acceptable salt, hydrate, or solvate in improving crop yield or quality.

[0011] In a fifth aspect, the present invention provides use of vanillin or a pharmaceutically acceptable salt, hydrate, or solvate thereof in the preparation of a pesticide; the pesticide is used to enhance crop resistance or resist stress.

[0012] In the present invention, the crop is a plant of the Poaceae family, acanthaceae family, or a plant of the Plantaginaceae family; preferably a plant of the genus Zea; more preferably corn.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] The present invention has found that vanillin can be used to induce plants to produce benzoxazine defense substances, enhance crop resistance, and resist stress. The present invention is to explore the allelopathic mechanism of plant interspecific interaction inducing plant resistance improvement, and provide a theoretical basis for the rational use of crop diversity planting. At the same time, the above discovery of vanillin can provide materials for the development of new biogenic inducers, which is of great value in reducing the use of other chemical pesticides. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a diagram of the experimental results of the vanillin provided by the present invention inducing corn to synthesize MBOA. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] If no specific techniques or conditions are specified in the examples, all are carried out according to conventional methods or the techniques or conditions described in the literature in the field, or according to the product instructions. If no manufacturer is specified for the reagents and instruments used, all are conventional products that can be purchased through regular channels.

[0018] In the following examples, high performance liquid chromatography (HPLC) was used to detect the content of MBOA, a resistance substance in corn plants.

[0019] Example

[0020] This example verifies the effect of vanillin on inducing the synthesis of MBOA in corn.

[0021] The specific method is: vanillin is prepared into solutions of 0 mg / L, 1 mg / L, 10 mg / L, 50 mg / L, 100 mg / L, and 200 mg / L, respectively, and after hydroponically treating corn plants for 48 hours, the changes in MBOA content in the corn plants are measured.

[0022] The results are as follows Figure 1 As shown in the figure, different lowercase letters indicate significant differences in the aboveground part (underground part) of corn plants between treatments with different concentrations of vanillin (P<0.05, n=3).

[0023] The results showed that vanillin could induce an increase in MBOA content in corn at a lower concentration (10 mg / L), and its effect on the MBOA content in the underground part of corn was more significant.

[0024] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

Application of 1.1-200 mg / L vanillin in inducing the synthesis of 6-methoxybenzoxazoline-2-one in corn.

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