A pesticide composition for preventing and treating Euryale ferox leaf gall disease and its application

Abstract

The present invention provides a pesticide composition for preventing and treating leaf tumor of Gorgon fruit and its application, belonging to the technical field of pesticides. The present invention provides a synergistic fungicidal composition for preventing and treating leaf tumor of Gorgon fruit, comprising tebuconazole and pyraclostrobin. The present invention uses tebuconazole and pyraclostrobin in combination as a synergistic fungicidal composition for preventing and treating leaf tumor of Gorgon fruit, and the combined use of tebuconazole and pyraclostrobin can achieve a synergistic technical effect in preventing and treating leaf tumor of Gorgon fruit. The results of the embodiments show that when the synergistic fungicidal composition for preventing and treating leaf tumor of Gorgon fruit provided by the present invention is applied to the early stage of infection of leaf tumor of Gorgon fruit, the prevention and treatment effect of leaf tumor is as high as 97%, and Gorgon fruit does not show symptoms of dwarfing, chlorosis and deformity.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating Euryale ferox leaf gall disease and application thereof. Background Art

[0002] The aquatic herb Euryale ferox Salisb. belongs to the genus Euryale (Nymphaeaceae) and is distributed in both the north and south of my country. Because its mature fruit is shaped like a chicken's beak, it is called "chicken head rice". Euryale ferox is the seed of the plant Euryale ferox. It tastes sweet and astringent, and is neutral in nature. It has the effects of nourishing the kidney and strengthening the essence, nourishing the spleen and stopping diarrhea, and removing dampness and stopping leukorrhea. It is a natural tonic that can be used as both medicine and food. However, with the increase in the cultivation area of ​​Euryale ferox, the disease problem has become increasingly serious, affecting the yield and quality of Euryale ferox, and restricting the production of Euryale ferox.

[0003] Leaf gall disease is a common disease on Euryale ferox, the pathogen is Fereydounia khargensis. It usually occurs from late June to the early stage of harvest. The lesions are small yellowish green spots in the early stage, and gradually expand to form tumors in the later stage. The maximum diameter of the tumor can reach 30cm and the thickness is 5-8cm. After the tumor is formed, it will affect the water discharge of Euryale ferox, affect the quality and yield of Euryale ferox, and reduce the yield by 10%-20% in mild years and 40%-60% in severe years.

[0004] Since Gorgon fruit is an aquatic crop, it is difficult to prevent and control Gorgon fruit leaf gall disease. Commonly used drugs in production, such as carbendazim, are commonly used drugs, which often cause the risk of drug resistance in bacteria, and the prevention and control effect is increasingly reduced. Therefore, currently providing a drug combination that can effectively prevent and control Gorgon fruit leaf gall disease without toxic side effects on plants is a technical problem that needs to be solved to improve the yield and quality of Gorgon fruit. Summary of the invention

[0005] In view of the defects in the prior art, the purpose of the present invention is to provide a synergistic fungicide composition for preventing and treating Euryale ferox leaf gall disease, which can effectively prevent and treat Euryale ferox leaf gall disease and is safe and has no side effects.

[0006] The objective of the present invention is achieved through the following technical solutions:

[0007] The invention provides a synergistic fungicide composition for preventing and treating Euryale ferox leaf gall disease, comprising tebuconazole and pyraclostrobin.

[0008] Preferably, the mass ratio of tebuconazole to pyraclostrobin is (1-5): (1-5).

[0009] Preferably, the mass ratio of tebuconazole to pyraclostrobin is 5:1.

[0010] The present invention provides a synergistic compound fungicide, comprising the synergistic composition described in the above technical solution and auxiliary ingredients acceptable to pesticides;

[0011] The auxiliary components include one or more of auxiliary agents, solvents or fillers.

[0012] Preferably, the auxiliary components include wetting and dispersing agents, thickeners, defoaming agents, antifreeze agents, preservatives and water.

[0013] Preferably, the synergistic compound fungicide comprises the following components in parts by weight: 0.01 to 20 parts of tebuconazole, 0.01 to 10 parts of pyraclostrobin, 1 to 10 parts of wetting and dispersing agent, 0.1 to 3 parts of thickener, 0.1 to 1 part of defoaming agent, 1 to 8 parts of antifreeze agent, 0.1 to 1 part of preservative and 50 to 60 parts of water.

[0014] Preferably, the dosage form of the synergistic fungicide includes a liquid preparation; the liquid preparation includes a suspension or a microcapsule suspension.

[0015] The present invention provides the use of the synergistic fungicide composition described in the above technical solution or the synergistic compound fungicide described in the above technical solution in inhibiting pathogenic bacteria of Euryale ferox leaf gall disease and / or preventing and controlling Euryale ferox leaf gall disease.

[0016] The present invention also provides a method for preventing and treating Euryale ferox leaf gall disease using the synergistic fungicide composition or the synergistic fungicide described in the above technical solution, comprising: applying the synergistic fungicide composition twice;

[0017] The first application of pesticide should be carried out at the early stage of the disease of Euryale ferox leaf gall.

[0018] The second application of pesticide should be carried out 7 to 10 days after the first application.

[0019] Preferably, the dosage of the synergistic fungicidal composition is 75-105 mL / hm 2 ; The application method of the synergistic fungicidal composition is spraying.

[0020] Beneficial effects:

[0021] The invention provides a synergistic fungicide composition for preventing and treating Euryale ferox leaf gall disease, comprising tebuconazole and pyraclostrobin. Tebuconazole is a highly efficient, broad-spectrum, systemic triazole fungicide pesticide, which can inhibit the biosynthesis of ergosterol of fungi, and has three functions of protecting, treating, and eradicating pathogenic microorganisms for plants, with a wide fungicide spectrum and a long lasting effect. Pyraclostrobin is a methoxyacrylate fungicide, which inhibits mitochondrial respiration and eventually causes cell death, and has the functions of protecting, treating, and leaf penetration and conduction, and is mainly used for preventing and treating various diseases caused by fungi on crops. Pyraclostrobin can also induce physiological phenomena of plants, such as improving the absorption of nitrogen, thereby promoting rapid growth of crops, increasing crop yields, and achieving the purpose of high crop yields. The present invention uses tebuconazole and pyraclostrobin as a synergistic fungicide composition for preventing and treating leaf tumor of Gorgon fruit. The combined use of tebuconazole and pyraclostrobin can achieve a synergistic technical effect in preventing and treating leaf tumor of Gorgon fruit. The results of the embodiment show that the synergistic fungicide composition for preventing and treating leaf tumor of Gorgon fruit provided by the present invention is applied to the early stage of infection of leaf tumor of Gorgon fruit, and the prevention and treatment effect of leaf tumor is as high as 97%, which is significantly higher than the prevention and treatment effect of the two agents used alone, and Gorgon fruit does not show dwarfing, chlorosis and deformity and other phytotoxic symptoms. DETAILED DESCRIPTION

[0022] The invention provides a synergistic fungicide composition for preventing and treating Euryale ferox leaf gall disease, comprising tebuconazole and pyraclostrobin.

[0023] The mass ratio of tebuconazole to pyraclostrobin in the fungicidal composition provided by the present invention is preferably (1-5):(1-5), more preferably (1-5):1, and more preferably 5:1.

[0024] In the present invention, tebuconazole (Tebuconazole) is a highly efficient, broad-spectrum, systemic triazole fungicide pesticide, which can inhibit the biosynthesis of ergosterol of fungi, and has three functions of protecting, treating and eradicating pathogenic microorganisms for plants, with a wide bactericidal spectrum and a long lasting effect. Pyraclostrobine (Pyraclostrobine) is a methoxyacrylate fungicide, which inhibits mitochondrial respiration, eventually leading to cell death, has protection, treatment, and leaf penetration and conduction effects, and is mainly used to prevent and treat various diseases caused by fungi on crops. Pyraclostrobine can also induce physiological phenomena of plants, such as improving the absorption of nitrogen, thereby promoting the rapid growth of crops, increasing crop yields, and achieving the purpose of high crop yields. The present invention uses tebuconazole and pyraclostrobine in combination to achieve a synergistic technical effect in preventing and controlling Euryale ferox leaf tumor disease. The present invention uses tebuconazole and pyraclostrobine in combination to reduce the incidence of Euryale ferox leaf tumor disease and improve the prevention and control effect of Euryale ferox leaf tumor disease in the field.

[0025] The present invention provides a synergistic compound fungicide, comprising the synergistic composition described in the above technical solution and auxiliary ingredients acceptable to pesticides;

[0026] The auxiliary components include one or more of auxiliary agents, solvents or fillers.

[0027] In the present invention, the auxiliary components preferably include a wetting and dispersing agent, a thickener, a defoaming agent, an antifreeze agent, a preservative and water. In the present invention, when the auxiliary components of the synergistic compound fungicide include the above components, the synergistic compound fungicide preferably includes the following components by mass: 0.01-20 parts of tebuconazole, 0.01-10 parts of pyraclostrobin, 1-10 parts of a wetting and dispersing agent, 0.1-3 parts of a thickener, 0.1-1 parts of a defoaming agent, 1-8 parts of an antifreeze agent, 0.1-1 parts of a preservative and 50-60 parts of water; the synergistic compound fungicide more preferably includes the following components by mass: 20 parts of tebuconazole; 4 parts of pyraclostrobin; 9 parts of a wetting and dispersing agent, 3 parts of a thickener, 1 part of a defoaming agent, 8 parts of an antifreeze agent, 1 part of a preservative, and 54 parts of water. The synergistic compound fungicide used in the embodiment of the present invention is prepared by 25% tebuconazole suspension and 25% pyraclostrobin microcapsule suspension according to different mass ratios.

[0028] In the present invention, the dosage form of the synergistic fungicide preferably includes a liquid preparation; the liquid preparation preferably includes a suspension or a microcapsule suspension.

[0029] The present invention provides the use of the synergistic fungicide composition or the synergistic compound fungicide described in the above technical solution in inhibiting and / or preventing the leaf gall disease of Gorgon euryale. In the present invention, the leaf gall disease pathogen of Gorgon euryale includes Fereydouniakhargensis.

[0030] The results of the embodiments of the present invention show that when tebuconazole and pyraclostrobin are used in different mass ratios to prevent and control the pathogenic bacteria of Euryale ferox leaf tumor, the effects are quite different. When tebuconazole and pyraclostrobin are used in a mass ratio of (5-1): (1-5), the two have good effects in preventing and controlling the pathogenic bacteria of Euryale ferox leaf tumor; when tebuconazole and pyraclostrobin are used in a mass ratio of 5:1, the two have a synergistic effect in preventing and controlling the pathogenic bacteria of Euryale ferox leaf tumor.

[0031] The present invention uses tebuconazole and pyraclostrobin in combination as a pesticide preparation for preventing and treating leaf tumor of Gorgon fruit, which can significantly improve the prevention and treatment effect of leaf tumor of Gorgon fruit, and the prevention effect is as high as 94% to 98%. At the same time, the use of tebuconazole and pyraclostrobin in combination as a pesticide preparation for preventing and treating leaf tumor of Gorgon fruit will not cause Gorgon fruit to produce dwarfing, chlorosis or deformity and other phytotoxic symptoms. Therefore, the present invention uses tebuconazole and pyraclostrobin as active ingredients for preventing and treating leaf tumor of Gorgon fruit, which can effectively prevent and treat leaf tumor of Gorgon fruit, and is safe to Gorgon fruit plants and has no side effects.

[0032] The present invention also provides a method for preventing and treating Euryale leaf gall disease using the synergistic fungicide composition or the synergistic fungicide described in the above technical solution, comprising: applying the synergistic fungicide composition twice; applying the first application at the early stage of Euryale leaf gall disease; and applying the second application 7 to 10 days after the first application. In the present invention, the application amount of the synergistic fungicide composition is preferably 75 to 105 mL / hm 2 , more preferably 85 to 105 mL / hm 2 , more preferably 105mL / hm 2 In the present invention, the application method of the synergistic fungicidal composition is preferably spraying.

[0033] In order to further illustrate the present invention, the technical solutions provided by the present invention are described in detail below in conjunction with embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0034] Example 1

[0035] Indoor bioassay.

[0036] Test agents: 97% tebuconazole technical and 98% pyraclostrobin technical, of which 97% tebuconazole technical was purchased from Jiangsu Qizhou Green Chemical Co., Ltd., and 98% pyraclostrobin technical was purchased from Jiangsu Qizhou Green Chemical Co., Ltd.

[0037] Test object: Fereydouniakhargensis (pathogen of Euryale ferox leaf gall disease), the pathogen was collected by Huaiyin Agricultural Science Research Institute in Xuhuai area of ​​Jiangsu Province at the Euryale ferox production base in Huai'an City, and was obtained and preserved through isolation, pathogenicity determination and molecular biological identification.

[0038] Tebuconazole technical and pyraclostrobin technical were used to prepare 10000 μg / mL tebuconazole mother solution and pyraclostrobin mother solution, respectively, and the solvent used was acetone. Tebuconazole mother solution and pyraclostrobin mother solution were compounded at volume ratios of 1:1, 1:2, 1:3, 1:4, 1:5, 5:1, 4:1, 3:1 and 2:1 (i.e., tebuconazole and pyraclostrobin were compounded at mass ratios of 1:1, 1:2, 1:3, 1:4, 1:5, 5:1, 4:1, 3:1 and 2:1), and the highest concentration of each treatment was first prepared, and then the mixture was diluted by 2 times to prepare different concentrations as shown in Table 1, wherein the concentration shown in Table 1 is the total concentration of tebuconazole and pyraclostrobin (the sum of the concentrations of tebuconazole and pyraclostrobin).

[0039] Indoor toxicity determination method: Determined by mycelium growth rate method. The drugs of different concentrations and proportions shown in Table 1 were mixed with glucose potato culture medium (200g potato, 20g glucose, 16g agar powder, prepared into 1L culture medium) and poured into a 9cm diameter disposable culture dish to make a drug-containing plate. Each concentration was repeated 3 times, and an equal amount of acetone was added as a control. Use a puncher with an inner diameter of 5mm to punch out the activated pathogen cake, transfer the cake to the center of the drug-containing plate, and place it in a 25℃ constant temperature incubator for dark culture.

[0040] Table 1 Concentrations of tebuconazole, pyraclostrobin and their compound agents

[0041]

[0042] When the control colony grows to two-thirds of the size of the culture dish, the colony diameter is measured by the cross method, and the inhibition rate is calculated in Excel. The inhibition rate calculation formula is as shown in formula (1); the EC is obtained by processing the data with DPS 50 , E.C. 50 The calculation formula is as shown in formula (2); the Wadley method was used to evaluate the combined toxicity of tebuconazole and pyraclostrobin on the mycelial growth of Euryale ferox leaf gall pathogen, and the calculation formula of the combined toxicity is as shown in formula (3).

[0043]

[0044]

[0045]

[0046] Among them, EC 50 (Exp) is the theoretical value, A and B are single doses of tebuconazole and pyraclostrobin, EC 50 (A) is the EC of tebuconazole 50 Actual value, EC 50 (B) is the EC of pyraclostrobin 50Actual value, a, b are the proportions of the corresponding single agent in the mixture, EC 50 (Obs) is the actual value. SR>1.5 is a synergistic effect; SR=0.5-1.5 is an additive effect; SR<0.5 is an antagonistic effect.

[0047] The results of the indoor toxicity assay are shown in Table 2.

[0048] Table 2 The combined toxicity of tebuconazole and pyraclostrobin against the pathogen of Euryale ferox leaf gall disease

[0049]

[0050] As can be seen from Table 2, when tebuconazole and pyraclostrobin are compounded at a mass ratio of 1:1, 1:2, 1:3, 1:4, 1:5, 5:1, 4:1, 3:1, and 2:1, the synergistic coefficient is greater than 0.5, which has an additive effect. Among them, when the mass ratio of tebuconazole to pyraclostrobin is 5:1, the synergistic coefficient is greater than 1.5, which is 1.5029, and has a synergistic effect. Therefore, the best mass ratio of 5:1 with a synergistic effect was selected for the field efficacy test.

[0051] Example 2

[0052] Field efficacy test1.

[0053] The test site is Cheqiao Town, Huai'an District, Huai'an City, Jiangsu Province (33°30′33"N, 119°20′16"E). The test field covers an area of ​​5 mu, with a soil pH of 7.2 and an organic matter content of 1.8%.

[0054] Drugs: 25% tebuconazole suspension and 25% pyraclostrobin microcapsule suspension, wherein 25% tebuconazole suspension was purchased from Jiangsu Jianpai Agrochemical Co., Ltd., and 25% pyraclostrobin suspension was purchased from Lianbao Crop Science and Technology Co., Ltd.

[0055] The test crop is Gorgon fruit, the variety is Green Gorgon, which was sown on May 10, 2022. Cultivation management is carried out in accordance with local scientific cultivation measures. The test target is leaf gall disease. The application method is conventional spraying, using Singapore Linong electric sprayer (model: Pjb-16, sprayer number: P-02), the nozzle is a hollow cone. Before application, the sprayer must be calibrated according to SOP / EO-01-V2.0 "Use, calibration and maintenance of sprayers".

[0056] The compound ratio with synergistic effect indoors was screened, i.e., tebuconazole and pyraclostrobin were used in a mass ratio of 5:1 for field efficacy test. The pesticides were applied twice, the first application time was at the early stage of the disease, and the second application time was 10 days after the first application. 25% tebuconazole suspension and 25% pyraclostrobin microcapsule suspension were applied separately as the control drug group, and the clean water treatment group was used as the control group. The dosage of the test group and the control group is shown in Table 3.

[0057] The first application time is August 1, 2022; the second application time is August 11, 2022, a total of 2 applications.

[0058] Table 3 Dosages of Tebuconazole and Pyraclostrobin compound and Tebuconazole and Pyraclostrobin alone

[0059]

[0060] Among them, 25% tebuconazole suspension and 25% pyraclostrobin microcapsule suspension are compounded in a volume ratio of 5:1 to obtain a ratio in which tebuconazole and pyraclostrobin are compounded in a mass ratio of 5:1. 25% tebuconazole suspension and 25% pyraclostrobin microcapsule suspension are compounded in a volume ratio of 5:1 to obtain a synergistic compound fungicide.

[0061] The synergistic compound fungicide was applied in the amounts described in Table 3. When applied, the synergistic compound fungicide was diluted. When applied, the synergistic compound fungicide was mixed with water and applied, and about 30 kg of water was applied per mu to dilute the synergistic compound fungicide.

[0062] In the first test, 75 mL of the synergistic compound fungicide was mixed with 450 kg of water and then applied to 1 hectare;

[0063] In test 2, 85 mL of the synergistic compound fungicide was mixed with 450 kg of water and applied to 1 hectare;

[0064] In test 3, 105 mL of the synergistic compound fungicide was mixed with 450 kg of water and applied to 1 hectare;

[0065] Control group 1: 112.5 mL of 25% tebuconazole suspension was mixed with 450 kg of water and applied to 1 hectare;

[0066] Control Group 2: 360 mL of 25% pyraclostrobin microcapsule suspension was mixed with 450 kg of water and applied to 1 hectare.

[0067] Each test was conducted in three parallel groups. 14 days after the second application of the pesticide, the incidence of Euryale ferox leaf gall disease was observed and counted, and the incidence rate, disease index and control effect of Euryale ferox leaf gall disease were calculated.

[0068] Field efficacy test: Disease classification standard of Euryale ferox leaf gall disease:

[0069] Level 0: no lesions;

[0070] Level 1: lesion area <5%;

[0071] Level 3: 6% ≤ lesion area < 15%;

[0072] Level 5: 16% ≤ lesion area < 25%;

[0073] Level 7: 26% ≤ lesion area < 50%;

[0074] Level 9: Lesion area ≥51%.

[0075] Disease index = [Σ(disease level × number of diseased leaves)] / (total number of leaves investigated × 9) × 100

[0076] Control effect = (disease index of control group - disease index of treatment group) / disease index of control group × 100%.

[0077] The statistical results of the field efficacy test in Cheqiao Town, Huai'an District, Huai'an City, Jiangsu Province are shown in Table 4.

[0078] Table 4 Statistical results of field efficacy of different test groups

[0079] Group Incidence (%) Disease index Control effect (%) Test Group 1 18.36 0.2058 94.31±1.09c Test Group 2 17.89 0.1956 96.49±0.86b Experiment 3 groups 10.23 0.1558 97.20±2.14a Control drug group 1 18.65 0.5143 90.77±0.63d Control drug group 2 20.58 0.8000 85.64±0.34e Control water group 95.21 5.5714 ——

[0080] As can be seen from Table 4, the combination of tebuconazole and pyraclostrobin can reduce the incidence of Euryale ferox leaf gall disease and improve the prevention effect in the field. The prevention effect survey results show that the dosage of the preparation of the present invention is 105mL / hm 2 The control effect was the best, with a control efficiency of 97.20%, significantly better than the control effect of the two single agents. Field observations showed that the above-mentioned test agents and control single agent treatments were safe for the test crop Euryale ferox, and no phytotoxicity symptoms (such as dwarfing, chlorosis and deformity, etc.) were found.

[0081] Example 3

[0082] Field efficacy test2.

[0083] Test location: Nanzha Town, Huai'an City, Jiangsu Province (33°29′49"N, 118°87′36"E).

[0084] The specific test crops, test agents, the settings of each test group, the application of the test agents, and the observation and statistics of the incidence of Euryale ferox leaf gall disease are the same as those in Example 2.

[0085] The statistical results of the field efficacy test in Nanzha Town, Huai'an City, Jiangsu Province are shown in Table 5.

[0086] Table 5 Statistical results of field efficacy of different test groups

[0087]

[0088]

[0089] As can be seen from Table 5, the combination of tebuconazole and pyraclostrobin can reduce the incidence of Euryale ferox leaf gall disease and improve the prevention effect in the field. The prevention effect survey results show that the dosage of the preparation of the present invention is 105mL / hm 2 The control effect was the best when the control efficiency was 96.52%, which was significantly better than the control effect of the two single agents. Field observations showed that the above-mentioned test agents and control single agent treatments were safe for the test crop Euryale ferox, and no symptoms of drug damage (such as dwarfing, chlorosis and deformity, etc.) were found.

[0090] In summary, the present invention uses a compound of tebuconazole and pyraclostrobin as a synergistic fungicidal composition for preventing and controlling Euryale ferox leaf tumor disease. The combined use of tebuconazole and pyraclostrobin can achieve a synergistic technical effect in preventing and controlling Euryale ferox leaf tumor disease, can reduce the incidence of Euryale ferox leaf tumor disease, and improve the prevention and control effect of Euryale ferox leaf tumor disease in the field.

[0091] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A synergistic fungicidal composition for preventing and treating Euryale ferox leaf gall disease in inhibiting pathogenic bacteria of Euryale ferox leaf gall disease and / or preventing and treating Euryale ferox leaf gall disease, wherein the synergistic fungicidal composition comprises tebuconazole and pyraclostrobin; The mass ratio of tebuconazole to pyraclostrobin is 5:

1.

2. The application according to claim 1, characterized in that: The synergistic fungicidal composition also includes pesticide-acceptable auxiliary ingredients; The auxiliary components include one or more of auxiliary agents, solvents or fillers.

3. The application according to claim 2, characterized in that: The auxiliary components include wetting and dispersing agents, thickeners, defoamers, antifreeze agents, preservatives and water.

4. The use according to claim 1, characterized in that: The synergistic fungicidal composition comprises the following components in parts by weight: 0.01 to 20 parts of tebuconazole, 0.01 to 10 parts of pyraclostrobin, 1 to 10 parts of a wetting and dispersing agent, 0.1 to 3 parts of a thickener, 0.1 to 1 part of a defoaming agent, 1 to 8 parts of an antifreeze agent, 0.1 to 1 part of a preservative and 50 to 60 parts of water.

5. The use according to claim 1, characterized in that: The dosage form of the synergistic bactericidal composition includes a liquid preparation; the liquid preparation includes a suspension or a microcapsule suspension.

6. A method for preventing and treating Euryale ferox leaf gall disease using a synergistic fungicidal composition, characterized in that: include: Apply the synergistic fungicidal composition twice; The first application of pesticide should be carried out at the early stage of the disease of Euryale ferox leaf gall. The second application of pesticide should be carried out 7 to 10 days after the first application; The synergistic fungicidal composition comprises tebuconazole and pyraclostrobin; The mass ratio of tebuconazole to pyraclostrobin is 5:

1.

7. The method according to claim 6, characterized in that The application amount of the synergistic bactericidal composition is 75-105 mL / hm 2 ; The application method of the synergistic fungicidal composition is spraying.

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