Anti-wrinkle and firming micro-nano essence lotion and preparation method thereof

Abstract

The invention discloses an anti-wrinkle firming micro-nano essence lotion and a preparation method thereof. The micro-nano essence lotion comprises the following components in parts by weight: 0.005-0.1 parts of ginseng tissue culture, 0.1-5 parts of PEG / PPG-70 / 30 tocopherol ether, 0.1-1 parts of hydrogenated lecithin, 3-12 parts of emollient oil, 3-15 parts of moisturizer, 0.0001-0.01 parts of red scorpion toxin peptide, 0.1-3 parts of ectoine, 0.05-2 parts of immortal grass extract, 0.01-2 parts of ergothioneine, 0.001-0.1 parts of acetyl hexapeptide-8, 0.001-0.1 parts of dipeptide diaminobutyryl benzylamide diacetate, 0.1-2 parts of hydrolyzed collagen, and 50-85 parts of deionized water. The present invention uses the synergistic effect of multiple anti-wrinkle and firming ingredients to combine immediate anti-wrinkle with long-term anti-wrinkle. Multiple effective moisturizing ingredients deeply replenish water and retain water on the surface. Long-term use can achieve the effects of lifting and firming, improving skin texture, brightening skin, and replenishing and moisturizing.

Description

Technical Field

[0001] The present invention belongs to the field of skin care products, and in particular, relates to an anti-wrinkle and firming micro-nano essence lotion and a preparation method thereof, and more particularly to an anti-wrinkle and firming micro-nano essence lotion containing ginseng tissue culture and a preparation method thereof. Background Art

[0002] As the physiological age increases, external pressure and environmental factors affect the skin, the cell metabolism of the skin slows down and the cell aging speed accelerates. As a result, with the loss of collagen, elastin and hyaluronic acid in the cells, the deep wrinkles, expression lines and skin sagging on the skin surface will become more serious year by year. Endogenous skin aging is one of the physiological characteristics of human metabolism and is an irreversible natural process. Exogenous aging refers to the aging process that occurs when the body is stimulated by the external environment and under the influence of long-term accumulation, such as ultraviolet radiation, pollution, smoking, drinking, long-term malnutrition, stress and other factors.

[0003] As people's requirements for quality of life improve, their awareness of skin protection is also gradually increasing. For example, by strengthening skin care, cleaning, moisturizing and sun protection, and using some firming, anti-wrinkle, anti-oxidant and skin-brightening skin care products, they hope to achieve the purpose of firming the skin, relieving the formation of skin wrinkles and brightening the skin, thereby slowing down the aging of the skin.

[0004] Elastase has the ability to degrade collagen, elastin and other proteins. Changes in the structure of elastin during skin aging are one of the important causes of wrinkles and skin sagging, and inhibiting elastase from decomposing elastin can play an anti-aging and anti-wrinkle role. Normal physiological metabolism of the human body and environmental influences, such as ultraviolet radiation and environmental pollution, can also lead to the generation of reactive oxygen species (ROS), and excessive free radicals can cause problems such as skin aging. Reducing the generation of free radicals and removing aging metabolites are also one of the methods to slow down skin aging and wrinkle formation.

[0005] Most of the anti-wrinkle ingredients added in existing anti-wrinkle and firming skin care products are often simply compounded, with a relatively simple mechanism of action, unable to play a systematic role, and unable to achieve a synergistic effect, resulting in mediocre anti-wrinkle effects. In addition, the time period required for the effects to take effect is long, and the effects are slow to take effect, or some products have short-term anti-wrinkle effects, but the duration is short, and there is no long-term anti-wrinkle effect; that is, it is impossible to achieve both immediate anti-wrinkle and long-lasting anti-wrinkle effects at the same time.

[0006] In view of this, the present invention is proposed. Summary of the invention

[0007] The technical problem to be solved by the present invention is to overcome the shortcomings of the prior art and provide a micro-nano essence milk and a preparation method thereof. The micro-nano essence milk contains ginseng tissue culture, and the multiple effective ingredients therein act synergistically to quickly improve skin wrinkles in a short period of time, achieving the effects of lifting and firming, combining immediate and long-term anti-wrinkle to improve skin wrinkles, brighten the skin, and replenish water and moisturize.

[0008] In order to solve the above technical problems, the basic concept of the technical solution adopted by the present invention is:

[0009] The present invention provides a micro-nano essence milk containing ginseng tissue culture, comprising the following components in parts by weight:

[0010] 0.005-0.1 parts of ginseng tissue culture, 0.1-5 parts of PEG / PPG-70 / 30 tocopherol ether, 0.1-1 parts of hydrogenated lecithin, 3-12 parts of emollients, 3-15 parts of moisturizers, 0.0001-0.01 parts of red scorpion toxin peptide, 0.1-3 parts of ectoine, 0.05-2 parts of immortal grass extract, 0.01-2 parts of ergothioneine, 0.001-0.1 parts of acetyl hexapeptide-8, 0.001-0.1 parts of dipeptide diaminobutyryl benzylamide diacetate, 0.1-2 parts of hydrolyzed collagen, and 50-85 parts of deionized water.

[0011] In the micro-nano essence emulsion of the present invention, PEG / PPG-70 / 30 tocopherol ether is combined with hydrogenated lecithin, and the structure of the micro-nano emulsion can be obtained by a conventional emulsification method. Compared with conventional ordinary emulsions, the droplet structure of the micro-nano emulsion is smaller, its stability is better, and it is more conducive to the long-term stable storage of skin care products; at the same time, the smaller the particle size droplets, the easier the nutrients are absorbed by the skin, and the absorption rate is also faster. The preparation of micro-nano emulsions generally requires expensive equipment and complex processes, and general atmospheric pressure emulsification methods, such as homogenizers, can only prepare conventional ordinary emulsions. In the present invention, PEG / PPG-70 / 30 tocopherol ether is combined with hydrogenated lecithin, and the structure of the micro-nano emulsion can be obtained by a normal pressure emulsification method.

[0012] Among them, PEG / PPG-70 / 30 tocopherol ether has an aromatic ring (VE) structure, and also has heterocyclic and branched chains, which can be better soluble in cosmetic oils and fats. The emulsified particles are smaller and more stable than conventional emulsifiers. Hydrogenated lecithin is a stable emulsifier formed by hydrogenation of lecithin under the action of a catalyst. It is a white free-flowing powder, odorless, and a new type of emulsifier that retains the active ingredients of lecithin intact. It has strong hydrophilicity and moisturizing properties, and has a strong affinity for the skin and mucous membranes. It can be used in cosmetic formulations to moisturize, emulsify, disperse, and anti-oxidize. The two complement each other, and through synergistic effects, they emulsify the moisturizing oils and fats together to form a micro-nano emulsion structure.

[0013] In a further embodiment, the mass ratio of PEG / PPG-70 / 30 tocopherol ether to hydrogenated lecithin is 1:(0.1-2),

[0014] Preferably, the mass ratio of PEG / PPG-70 / 30 tocopherol ether: hydrogenated lecithin is 1:0.5.

[0015] When the mass ratio of PEG / PPG-70 / 30 tocopherol ether: hydrogenated lecithin is 1:(0.1-2), the two have a better synergistic effect, and the particle size distribution range of the prepared nano-microemulsion is appropriate. When the mass ratio of the two is 1:0.5, the effect is best. If there is too much hydrogenated lecithin, the auxiliary emulsifier is too high, the HLB value is too high, the emulsification effect becomes worse, and the droplet size will be larger; if the amount of hydrogenated lecithin is too little, the auxiliary emulsifier is less, and the synergistic effect and the effect of reducing the droplet size cannot be achieved.

[0016] In a further solution, the particle size distribution range of the obtained nano-microemulsion is: D50: 100-300nm, D90: 350-500nm. In this way, the prepared nano-microemulsion has a small particle size, good stability, and the nutrients are more easily absorbed by the skin, the emulsion is absorbed faster, and the skin feels more refreshing.

[0017] In a further embodiment, the mass ratio of red scorpion toxin peptide, acetyl hexapeptide-8, and dipeptide diaminobutyryl benzylamide diacetate is 1:(5-10):(2-5);

[0018] Preferably, the mass ratio of red scorpion toxin peptide, acetyl hexapeptide-8, and dipeptide diaminobutyryl benzylamide diacetate is 1:8:3.

[0019] The micro-nano essence milk of the present invention adopts three instant anti-wrinkle peptides with different mechanisms of action, namely, red scorpion toxin peptide, acetyl hexapeptide-8, and dipeptide diaminobutyryl benzylamide diacetate. The three cooperate to work synergistically. Among them, red scorpion toxin can effectively relax facial muscles, and the facial expression is more natural while instant anti-wrinkle; acetyl hexapeptide-8 acts on muscle contraction signal conduction to reduce muscle contraction force; dipeptide diaminobutyryl benzylamide diacetate mainly acts on instant tightening of the surface skin; by relaxing facial muscles, reducing muscle contraction force and tightening the surface skin state, the three are carried out simultaneously, thereby achieving better instant anti-wrinkle effect. Red scorpion toxin peptide is a bio-simulated conotoxin peptide, which provides a very strong muscle relaxation effect after sorting and artificial synthesis, and provides a rapid and instant anti-wrinkle effect. Red scorpion toxin provides effective muscle relaxation but not completely stiff and paralyzed, retaining 5% of the neuromuscular current conduction of the muscle, so it provides a natural rejuvenation effect. Red scorpion toxin is a highly folded peptide with strong penetration, high stability, and targeted relaxation effect on muscles. Acetyl hexapeptide-8 is a neurotransmitter inhibitory peptide that is suitable for reducing wrinkles caused by facial muscle contraction, reducing the strength of facial muscle contraction, and relaxing facial muscles, especially those near the eyes and forehead, similar to the effect of botulinum toxin. Dipeptide diaminobutyryl benzylamide diacetate is a synthetic peptide that simulates snake venom serum, which can block the nerve conduction of acetylcholine, make loose skin firmer and more elastic, and thus improve dynamic wrinkles.

[0020] The present invention finds that when the mass ratio of red scorpion toxin peptide, acetyl hexapeptide-8, and dipeptide diaminobutyryl benzylamide diacetate is 1:(5-10):(2-5), the three instant anti-wrinkle peptides have a better synergistic effect and an excellent instant wrinkle removal effect. When the mass ratio is 1:8:3, the effect is optimal.

[0021] In a further embodiment, the mass ratio of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and chrysanthemum officinale extract is 1:(5-20):(5-20):(10-30):(10-30),

[0022] Preferably, the mass ratio of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and herbaceous vine extract is 1:15:15:20:25.

[0023] In the anti-wrinkle and firming micro-nano essence lotion of the present invention, multiple anti-wrinkle and firming functional ingredients of ginseng tissue culture + hydrolyzed collagen + ergothioneine + ectoine + immortal grass extract synergize, ginseng tissue culture can deeply supplement and promote the synthesis of collagen and elastin, ergothioneine has very excellent antioxidant and free radical scavenging effects, ectoine and immortal grass extract have the ability to protect epidermal cell activity, increase skin cell vitality, and promote skin cell metabolism; and then the five anti-wrinkle and firming raw materials act simultaneously from multiple aspects to achieve better long-term anti-wrinkle and firming effects. As a beauty and skin care product, ginseng can not only improve the activity of skin antioxidant enzymes, increase collagen content, inhibit the activity of metalloproteinase MMP-1, slow down collagen loss, reduce skin wrinkles, but also protect ultraviolet rays from causing damage to keratinocytes and dermal fibroblasts. Ergothioneine is a natural amino acid that exists naturally in animals and plants. It is rich in content. It cannot be synthesized by the animal body itself and can only be taken from food. It is a rare amino acid. Ergothioneine plays an important role in the synthesis of body cells and has strong antioxidant properties. It is also a cell physiological protector with multiple physiological functions such as scavenging free radicals, anti-aging, anti-radiation, and maintaining normal cell growth. Ecdoin comes from highly halophilic bacteria, so Ecdoin is called "halophilic bacteria extract". Under extreme conditions of high salt, high temperature, and high ultraviolet radiation, Ecdoin protects halophilic bacteria from harm. Research results show that Ecdoin also has good repair, protection, anti-aging, moisturizing, and sun protection effects on the skin. Hydrolyzed collagen is also called collagen peptides or collagen powder, not just protein powder: it is rich in amino acids needed by the human body, can build and repair skin cells, and replenish all the amino acids required for skin collagen synthesis, so as to increase skin moisture and skin elasticity. The extract of immortal grass has been scientifically studied abroad. Its main ingredient is glyceryl glucoside, which can protect the stability of cell membranes and cell structures. It has excellent water-locking ability similar to hyaluronic acid, and stimulates cell vitality from multiple aspects such as metabolism, anti-oxidation, and collagen synthesis.

[0024] In a further embodiment, the anti-wrinkle and firming micro-nano essence lotion further comprises the following ingredients in parts by weight:

[0025] Palmitoyl tetrapeptide-7 0.001-0.1 parts, palmitoyl pentapeptide-4 0.001-0.1 parts, palmitoyl tripeptide-5 0.001-0.1 parts, hexapeptide-9 0.001-0.1 parts.

[0026] The anti-wrinkle and firming micro-nano essence lotion of the present invention also includes a variety of peptides, which further promote protein synthesis or inhibit collagen degradation, further improve the anti-wrinkle and firming effect, ensure long-term anti-wrinkle effect, and improve skin.

[0027] In a further embodiment, in the anti-wrinkle and firming micro-nano essence lotion, the emollient oil comprises the following ingredients in parts by weight: 0.5-5 parts of squalane, 1-5 parts of isononyl isononanoate, 1-5 parts of polydimethylsiloxane, and 0.01-1 parts of ubiquinone;

[0028] The moisturizing agent comprises the following components in parts by weight:

[0029] Betaine 0.5-3 parts, panthenol 0.1-2 parts, acetyl glucosamine 0.1-3 parts, sodium hyaluronate 0.01-1 parts, hydrolyzed sodium hyaluronate 0.01-1 parts, 1,2-pentanediol 1-5 parts, butylene glycol 2-6 parts, trehalose 0.1-3 parts.

[0030] The micro-nano essence milk of the present invention also includes a variety of lubricating, moisturizing and anti-oxidation ingredients, which promote the expression of aquaporin, replenish moisture in the deep layer, retain moisture on the surface layer, and enhance the moisturizing ability of skin cells.

[0031] That is, the anti-wrinkle and firming micro-nano essence lotion containing ginseng tissue culture of the present invention comprises the following components in parts by weight:

[0032] 0.005-0.1 parts of ginseng tissue culture, 0.1-5 parts of PEG / PPG-70 / 30 tocopherol ether, 0.0001-0.01 parts of red scorpion toxin peptide, 0.5-5 parts of squalane, 0.5-3 parts of betaine, 0.1-3 parts of ectoine, 0.1-2 parts of panthenol, 0.1-3 parts of acetyl glucosamine, 0.05-2 parts of immortal grass extract, 0.1-1 parts of hydrogenated lecithin, 0.01-1 parts of sodium hyaluronate, 0.01-2 parts of ergothioneine, 0.01-1 parts of hydrolyzed sodium hyaluronate, 1-5 parts of 1,2-pentanediol, 2-6 parts of butylene glycol, 1-5 parts of isononyl isononanoate, 0.1-3 parts of trehalose, 1-5 parts of polydimethylsiloxane, palmitoyl tetrapeptide-7 0.001-0.1 parts, palmitoyl pentapeptide-4 0.001-0.1 parts, palmitoyl tripeptide-5 0.001-0.1 parts, hexapeptide-9 0.001-0.1 parts, acetyl hexapeptide-8 0.001-0.1 parts, dipeptide diaminobutyroyl benzylamide diacetate 0.001-0.1 parts, hydrolyzed collagen 0.1-2 parts, ubiquinone 0.01-1 parts, deionized water 50-85 parts.

[0033] As a preferred embodiment, the anti-wrinkle and firming micro-nano essence lotion containing ginseng tissue culture of the present invention comprises the following components in parts by weight:

[0034] 0.05 parts of ginseng tissue culture, 0.8 parts of PEG / PPG-70 / 30 tocopherol ether, 0.01 parts of red scorpion toxin peptide, 3 parts of squalane, 3 parts of betaine, 1 part of ectoine, 1 part of panthenol, 2 parts of acetyl glucosamine, 1.25 parts of immortal grass extract, 0.4 parts of hydrogenated lecithin, 0.5 parts of sodium hyaluronate, 0.75 parts of ergothioneine, 0.5 parts of hydrolyzed sodium hyaluronate, 4 parts of 1,2-pentanediol, 4 parts of butylene glycol, 3 parts of isononyl isononanoate, 1 part of trehalose, 3 parts of polydimethylsiloxane, 0.1 parts of palmitoyl tetrapeptide-7, 0.1 parts of palmitoyl pentapeptide-4, 0.1 parts of palmitoyl tripeptide-5, 0.1 parts of hexapeptide-9, acetyl hexapeptide-8 0.08 parts, 0.03 parts of dipeptide diaminobutyryl benzylamide diacetate, 0.75 parts of hydrolyzed collagen, 0.05 parts of ubiquinone, and deionized water are added to make the total amount 100 parts.

[0035] When the weight proportions of the components are as mentioned above, the skin texture and wrinkle improvement effects are better, and the lifting and firming effects are better.

[0036] In addition, in the present invention, the ginseng tissue culture is a tissue-cultured ginseng adventitious root, which can be obtained by cultivation using the methods in the prior art. As a preferred method, the ginseng tissue culture in the present invention can adopt the method of cultivating ginseng adventitious roots in the invention patents CN 112931216, CN112806267, and CN 113186150 authorized by the applicant, and the present invention will not repeat them. Then the ginseng adventitious roots obtained by tissue culture are placed in a 40°C blast dryer, dried for 4-8 hours, heated to 45-50°C, and continued to dry for 5-10 hours; then cooled to 35-40°C, low-temperature blast drying for 10-15 hours until the moisture content reaches less than 10%, then stopped drying and cooled to room temperature. Adopting a medium-low temperature interval method, blasting or air circulation method for drying can prevent the ginseng adventitious roots from discoloring and losing nutrients to the greatest extent. The dried ginseng adventitious roots are crushed and sieved by an ultrafine grinder to obtain 200-400 mesh ginseng tissue culture, which has a high content of ginsenosides and a high content of rare ginsenosides.

[0037] The micro-nano essence milk containing ginseng tissue culture of the present invention has multiple effective moisturizing ingredients added to the product, which can enhance the skin's water retention capacity and has a certain effect of brightening the skin. For example, immortal grass extract, acetyl glucosamine, hyaluronic acid, panthenol, etc. can promote the expression of aquaporin, deeply replenish water, retain water on the surface, and enhance the moisturizing capacity of skin cells.

[0038] In the present invention, the elastase inhibition rate of the micro-nano essence milk diluted to 50% is 10-30%, and the scavenging rate of DPPH free radicals is 20-40%, which further proves that the product has good firming and anti-wrinkle effects.

[0039] The present invention also provides a method for preparing a micro-nano essence milk containing ginseng tissue culture, comprising the following steps:

[0040] 1 Water phase: add moisturizer and immortal grass extract to 90% of deionized water, heat to 80-85℃, and stir to fully disperse;

[0041] S2 oil phase: Stir and heat PEG / PPG-70 / 30 tocopherol ether, hydrogenated lecithin, and emollient oil to melt and disperse evenly, and heat the oil phase to 80-85°C and maintain;

[0042] S3: Mix the oil phase and the water phase, start homogenization and stirring for 10-15 minutes, and maintain the temperature at 80-85°C;

[0043] S4: After homogenization, cool to 45-40°C, add ginseng tissue culture, red scorpion toxin peptide, ectoine, ergothioneine, acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, and hydrolyzed collagen, stir evenly, and add the remaining 10% deionized water to 100 parts.

[0044] In a further embodiment, the preparation method comprises the following steps:

[0045] S1 aqueous phase: add butylene glycol, pentylene glycol, trehalose, betaine, panthenol, acetyl glucosamine, celery extract, sodium hyaluronate, and hydrolyzed sodium hyaluronate to 90% deionized water, heat to 80-85°C, and stir to fully disperse;

[0046] S2 oil phase: Stir and heat squalane, PEG / PPG-70 / 30 tocopherol ether, polydimethylsiloxane, isononyl isononanoate, hydrogenated lecithin, and ubiquinone to melt and disperse evenly, and heat the oil phase to 80-85°C and maintain;

[0047] S3: Mix the oil phase and the water phase, start homogenization and stirring for 10-15 minutes, and maintain the temperature at 80-85°C;

[0048] S4: After homogenization, cool to 45-40°C, add ginseng tissue culture, red scorpion toxin peptide, ectoine, ergothioneine, acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, hydrolyzed collagen, palmitoyl tetrapeptide-7, palmitoyl pentapeptide-4, palmitoyl tripeptide-5, hexapeptide-9 and mix well, and add the remaining 10% deionized water to 100 parts.

[0049] After adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art:

[0050] 1. The dosage form of the present invention adopts a micro-nano emulsion texture, adds PEG / PPG-70 / 30 tocopherol ether and hydrogenated lecithin, and adopts a normal pressure emulsification method to obtain the structure of the micro-nano emulsion. Among them, PEG / PPG-70 / 30 tocopherol ether has an aromatic ring (VE) structure, and at the same time has heterocyclic rings and side chains, which can be better soluble with cosmetic oils and fats. The emulsified particles are smaller and more stable than conventional emulsifiers. The two complement each other, and through synergistic effects, they emulsify the moisturizing oils and fats together to form a micro-nano emulsion structure. The particle size distribution range of the obtained nano-microemulsion is: D50: 100-300nm, D90: 350-500nm. In this way, not only the equipment used is simple, the operation is simple, and the cost is low, but also the micro-nano emulsion has high stability, and the effective ingredients are easier to absorb, which is conducive to maintaining a better firming and wrinkle-removing effect.

[0051] 2. In the anti-wrinkle and firming micro-nano essence lotion of the present invention, three instant anti-wrinkle peptides with different action mechanisms, namely, red scorpion toxin peptide, acetyl hexapeptide-8, and dipeptide diaminobutyryl benzylamide diacetate, can synergistically enhance their effectiveness under a certain mass ratio to achieve an effect of 1+1+1>3, thereby achieving a better instant anti-wrinkle effect.

[0052] 3. The anti-wrinkle and firming micro-nano essence lotion of the present invention contains ginseng tissue culture + hydrolyzed collagen + ergothioneine + ectoine + immortal grass extract. Multiple anti-wrinkle and firming ingredients produce a synergistic effect under a certain mass ratio, deeply supplementing and promoting the synthesis of collagen and elastin from different levels, anti-oxidation, while protecting the activity of epidermal cells, enhancing cell vitality, and promoting skin cell metabolism, so as to achieve better long-term anti-wrinkle and firming effects.

[0053] 4. The anti-wrinkle and firming micro-nano essence lotion of the present invention also includes a variety of peptides to further promote protein synthesis or inhibit collagen degradation, further improve the anti-wrinkle and firming effect, ensure long-term anti-wrinkle effect, and improve the skin; as well as a variety of lubricating, moisturizing, and antioxidant ingredients that can promote the expression of aquaporin, deeply replenish water, retain water on the surface, and enhance the moisturizing ability of skin cells.

[0054] The specific implementation modes of the present invention are further described in detail below in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0055] The accompanying drawings are part of the present invention and are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention, but do not constitute an improper limitation of the present invention. Obviously, the drawings described below are only some embodiments. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. In the accompanying drawings:

[0056] Figure 1 It is a schematic diagram of the particle size distribution of the micro-nano emulsion of the present invention.

[0057] It should be noted that these drawings and textual descriptions are not intended to limit the conceptual scope of the present invention in any way, but are intended to illustrate the concept of the present invention for those skilled in the art by referring to specific embodiments. DETAILED DESCRIPTION

[0058] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. The following embodiments are used to illustrate the present invention but are not used to limit the scope of the present invention.

[0059] The ginseng tissue culture in the embodiments or test examples of the present invention is prepared by the method of Example 24 of the invention patent of the applicant's publication number CN112806267. The ginseng adventitious roots are placed in a 40°C blast dryer, dried for 5 hours, then heated to 45°C and continued to dry for 6 hours; then cooled to 35°C, low-temperature blast drying for 10 until the moisture content reaches less than 10%, then stopped drying and cooled to room temperature. Then, after being crushed and sieved by an ultrafine grinder, 200-400 mesh ginseng tissue culture is obtained.

[0060] It should be noted that the use of the above-mentioned ginseng adventitious roots is only a specific implementation method, which is for illustration and does not limit the scope of the present application.

[0061] Example 1

[0062] A micro-nano essence milk containing ginseng tissue culture comprises the following components in parts by weight: 0.02 parts of ginseng tissue culture, 0.8 parts of PEG / PPG-70 / 30 tocopherol ether, 0.005 parts of red scorpion toxin peptide, 2 parts of squalane, 2 parts of betaine, 0.3 parts of ectoine, 0.5 parts of panthenol, 1 part of acetyl glucosamine, 0.4 parts of immortal grass extract, 0.4 parts of hydrogenated lecithin, 0.15 parts of sodium hyaluronate, 0.2 parts of ergothioneine, 0.05 parts of hydrolyzed sodium hyaluronate, 2 parts of 1,2-pentanediol, 2 parts of butylene glycol, 2 parts of isononyl isononanoate, 0.5 parts of trehalose, 3 parts of polydimethylsiloxane, 0.02 parts of palmitoyl tetrapeptide-7, 0.02 parts of palmitoyl pentapeptide-4, 0.02 parts of palmitoyl tripeptide-5, 0.02 parts of hexapeptide-9, and acetyl hexapeptide-8 0.03 parts, 0.01 parts of dipeptide diaminobutyryl benzylamide diacetate, 0.2 parts of hydrolyzed collagen, 0.01 parts of ubiquinone, and deionized water are added to make the total amount 100 parts.

[0063] The preparation method comprises the following steps:

[0064] S1 aqueous phase: add butylene glycol, pentylene glycol, trehalose, betaine, panthenol, acetyl glucosamine, celery extract, sodium hyaluronate, and hydrolyzed sodium hyaluronate into 90% deionized water, heat to 85°C, and stir to fully disperse.

[0065] S2 oil phase: Stir and heat squalane, PEG / PPG-70 / 30 tocopherol ether, polydimethylsiloxane, isononyl isononanoate, hydrogenated lecithin, and ubiquinone to melt and disperse evenly, and heat the oil phase to 85°C and maintain.

[0066] S3: Mix the oil phase and the water phase, start homogenization and stirring for 10-15 minutes, and maintain the temperature at 80-85℃.

[0067] S4: After homogenization, cool to 45-40°C, add ginseng tissue culture, red scorpion toxin peptide, ectoine, ergothioneine, acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, hydrolyzed collagen, palmitoyl tetrapeptide-7, palmitoyl pentapeptide-4, palmitoyl tripeptide-5, hexapeptide-9 and mix well, and add the remaining 10% deionized water to 100 parts.

[0068] Example 2

[0069] A micro-nano essence milk containing ginseng tissue culture comprises the following components in parts by weight: 0.05 parts of ginseng tissue culture, 0.8 parts of PEG / PPG-70 / 30 tocopherol ether, 0.01 parts of red scorpion toxin peptide, 3 parts of squalane, 3 parts of betaine, 1 part of ectoine, 1 part of panthenol, 2 parts of acetyl glucosamine, 1.25 parts of immortal grass extract, 0.4 parts of hydrogenated lecithin, 0.5 parts of sodium hyaluronate, 0.75 parts of ergothioneine, 0.5 parts of hydrolyzed sodium hyaluronate, 4 parts of 1,2-pentanediol, 4 parts of butylene glycol, 3 parts of isononyl isononanoate, 1 part of trehalose, 3 parts of polydimethylsiloxane, 0.1 parts of palmitoyl tetrapeptide-7, 0.1 parts of palmitoyl pentapeptide-4, 0.1 parts of palmitoyl tripeptide-5, 0.1 parts of hexapeptide-9, and acetyl hexapeptide-8 0.08 parts, 0.03 parts of dipeptide diaminobutyryl benzylamide diacetate, 0.75 parts of hydrolyzed collagen, 0.05 parts of ubiquinone, and deionized water are added to make the total amount 100 parts.

[0070] Preparation method: The preparation method is the same as that in Example 1 and will not be described in detail.

[0071] Example 3

[0072] A micro-nano essence milk containing ginseng tissue culture comprises the following components in parts by weight: 0.05 parts of ginseng tissue culture, 0.8 parts of PEG / PPG-70 / 30 tocopherol ether, 0.01 parts of red scorpion toxin peptide, 3 parts of squalane, 2.5 parts of betaine, 1.5 parts of ectoine, 2 parts of panthenol, 3 parts of acetyl glucosamine, 1.5 parts of immortal grass extract, 0.4 parts of hydrogenated lecithin, 1 part of sodium hyaluronate, 1 part of ergothioneine, 1 part of hydrolyzed sodium hyaluronate, 3 parts of 1,2-pentanediol, 5 parts of butylene glycol, 3 parts of isononyl isononanoate, 3 parts of trehalose, 3 parts of polydimethylsiloxane, 0.06 parts of palmitoyl tetrapeptide-7, 0.06 parts of palmitoyl pentapeptide-4, 0.06 parts of palmitoyl tripeptide-5, 0.06 parts of hexapeptide-9, and acetyl hexapeptide-8 0.1 parts, dipeptide diaminobutyryl benzylamide diacetate 0.05 parts, hydrolyzed collagen 1 parts, ubiquinone 0.06 parts, and deionized water are added to make the total amount 100 parts.

[0073] Preparation method: The preparation method is the same as that in Example 1 and will not be described in detail.

[0074] The effect of the micro-nano essence emulsion containing ginseng tissue culture of the present invention is analyzed by experiments.

[0075] Experimental effect example 1: Testing the effect of the micro-nano essence lotion of the present invention on improving skin texture

[0076] 30 healthy participants aged 25-55 were selected and divided into 3 groups on average. The participants were trained by professionals and the experiment was conducted. The participants had no history of skin diseases. During the test, they were required to use products provided by professionals and not apply any drugs or cosmetics unrelated to the experiment. Before using the product, clean the face with the same cleanser, dry the face, and use the VISIA detector to take pictures and analyze the initial value of the skin texture before use. Then use it once in the morning and evening every day. Clean the face before using the essence lotion and use the same moisturizing water; use the same moisturizing cream after using the essence lotion. According to the above-mentioned usage method, after one month of trial, clean the face with the cleanser again, dry it, and use the VISIA detector again to take pictures and analyze the skin texture value after use. The changes in skin texture before and after the use of the mask were obtained through comparative analysis.

[0077] Group 1 selected the essence milk obtained in Example 1 for testing, and obtained the data in Table 1; Group 2 selected the essence milk obtained in Example 2 for testing, and obtained the data in Table 2; Group 3 selected the essence milk obtained in Example 3 for testing, and obtained the data in Table 3.

[0078] All experimental data in this experiment were obtained under the same conditions. The VISIA tester took photos and analyzed the skin texture features to measure the number. The smaller the value, the better. The smaller the value, the smoother and more delicate the skin surface.

[0079] Table 1: Quantitative values ​​of skin texture characteristics before and after using the essence lotion prepared in Example 1

[0080] Experiencer Initial Value Test value Change value No. 1 2420 1928 -492 No. 2 2162 1725 -437 No.3 1028 824 -204 No. 4 3012 2769 -243 No. 5 1967 1427 -540 No. 6 2264 1978 -286 No.7 1845 1726 -119 No. 8 2009 1746 -263 No. 9 3054 2679 -375 No. 10 1649 1426 -223

[0081] Table 2: Quantitative values ​​of skin texture characteristics before and after using the essence lotion prepared in Example 2

[0082]

[0083]

[0084] Table 3: Quantitative values ​​of skin texture characteristics before and after using the essence lotion prepared in Example 3

[0085] Experiencer Initial Value Test value Change value No. 21 2185 1796 -389 No. 22 1876 1354 -522 No. 23 2598 2177 -421 No. 24 1888 1499 -389 No. 25 2471 2011 -460 No. 26 3276 2596 -680 No. 27 1968 1642 -326 No. 28 1856 1411 -445 No. 29 2019 1644 -375 No. 30 1866 1531 -335

[0086] It can be seen from the results in Tables 1, 2 and 3 that after using the essence lotion of the present invention, the test values ​​of the skin texture characteristic quantity of the test subjects were all lower than the initial values; that is, the skin smoothness was improved to varying degrees; this illustrates the effect of the essence lotion of the present invention in smoothing the skin.

[0087] Experimental effect example 2: Detection of the anti-wrinkle and firming effect of the micro-nano essence lotion of the present invention

[0088] Due to the lack of collagen and elastin, the skin structure collapses, causing the skin to sag and droop, resulting in true wrinkles. The VISIA detector can reflect the condition of skin wrinkles by taking photos and analyzing them. This experiment tested the changes in skin wrinkles before and after the experiencer used the essence lotion. The skin wrinkle test data of this experiment was obtained by the American VISIA detector.

[0089] The test was conducted according to the test method and test process of Experimental Effect Example 1, and the changes in skin wrinkles before and after the use of the essence lotion were obtained through comparative analysis.

[0090] Group 1 selected the essence milk obtained in Example 1 for testing, and obtained the data in Table 4; Group 2 selected the essence milk obtained in Example 2 for testing, and obtained the data in Table 5; Group 3 selected the essence milk obtained in Example 3 for testing, and obtained the data in Table 6.

[0091] All experimental data in this experiment were obtained under the same conditions. The VISIA tester took photos and analyzed them to measure the number of skin wrinkle characteristics. The smaller the value, the better. The smaller the value, the fewer skin wrinkles.

[0092] Table 4: Changes in skin wrinkles before and after using the essence lotion prepared in Example 1

[0093] Experiencer Initial Value Test value Change value No. 1 106 98 -8 No. 2 127 104 -23 No.3 87 79 -8 No. 4 64 52 -12 No. 5 113 96 -17 No. 6 110 99 -11 No.7 82 75 -7 No. 8 94 86 -8 No. 9 91 78 -13 No. 10 72 58 -14

[0094] Table 5: Changes in skin wrinkles before and after using the essence lotion prepared in Example 2

[0095] Experiencer Initial Value Test value Change value No. 11 89 44 -45 No. 12 120 88 -32 No. 13 58 27 -31 No. 14 105 84 -21 No. 15 118 81 -37 No.16 149 102 -47 No. 17 86 52 -34 No.18 92 62 -30 No.19 129 91 -38 No. 20 67 38 -29

[0096] Table 6: Changes in skin wrinkles before and after using the essence lotion prepared in Example 3

[0097]

[0098]

[0099] From the results of Table 4, Table 5 and Table 6, it can be seen that after using the essence lotion of the present invention, the test values ​​of the number of skin wrinkle characteristics of the test subjects are all lower than the initial values; that is, the skin wrinkle problems are improved to varying degrees; this shows that the essence lotion of the present invention has the effect of lifting, firming and reducing wrinkles.

[0100] According to the experience data analysis in Tables 1-6, the improvement effect of the essence lotion of Example 2 is significantly better than that of Example 3 and Example 1 in terms of the improvement effect of the experiencers' texture and wrinkles. The proportion of each component used in Example 1 is smaller than that in Example 2, while that in Example 3 is higher, which shows that under the combined ingredient ratio of Example 2, the various ingredients work synergistically to achieve better firming, anti-wrinkle and improvement of skin texture.

[0101] Experimental effect example 3: Detection of the particle size of the essence milk of the present invention

[0102] The particle size distribution of the essence sample of Example 2 was tested, and the test results are as follows:

[0103] Table 7 Particle size distribution of micro-nano essence milk

[0104]

[0105] It can be seen from Table 7 that the particle size D50 of the micro-nano emulsion of the present invention is 134 nm, and D90 is 277 nm; when the emulsifier PEG / PPG-70 / 30 tocopherol ether is combined with hydrogenated lecithin in a ratio of 1:0.5, after a normal pressure emulsification method at 80-85°C and homogenization for 10-15 minutes, a micro-nano emulsion with a particle size of about 200 nm can be achieved.

[0106] In this way, the smaller the particle size of the prepared nano-microemulsion, the better the stability, the nutrients are easier to be absorbed by the skin, the emulsion is absorbed faster and the skin feels more refreshing when used.

[0107] In addition, with reference to the prescription and preparation method of Example 2, the mass ratio of PEG / PPG-70 / 30 tocopherol ether to hydrogenated lecithin was adjusted, wherein the addition amount of PEG / PPG-70 / 30 tocopherol ether was 0.8 parts, and the addition amount of hydrogenated lecithin was 0.08 parts, 0.4 parts, 0.8 parts and 1.6 parts, respectively, to investigate the effect of the mass ratio of PEG / PPG-70 / 30 tocopherol ether to hydrogenated lecithin on the particle size of the emulsion. The results are shown in Table 8.

[0108] Table 8

[0109]

[0110]

[0111] Result analysis: Through the test data of particle size distribution of essence milk prepared by emulsifiers with different mass ratios, it can be seen that the particle size of essence milk is the smallest under the mass ratio of PEG / PPG-70 / 30 tocopherol ether and hydrogenated lecithin at a mass ratio of 1:0.5, and D50 can reach about 200nm. It further shows that when there is too much hydrogenated lecithin, the emulsification effect becomes worse and the droplet size will be larger; if the amount of hydrogenated lecithin is too little, there will be less auxiliary emulsifier, and it is impossible to achieve synergistic effect and reduce the droplet size; therefore, PEG / PPG-70 / 30 tocopherol ether and hydrogenated lecithin have a synergistic effect.

[0112] Experimental Effect Example 4: Detecting the efficacy of the essence milk of the present invention through in vitro experiments

[0113] The essence milk of Example 2 of the present invention was subjected to in vitro tests for elastase inhibition rate and DPPH free radical scavenging rate.

[0114] Elastase inhibition test: After the essence milk is diluted to 50% emulsion with buffer, it is mixed with elastase and incubated at 25°C for 10 minutes. The absorbance value is measured by ultraviolet spectrophotometer for analysis to obtain the essence milk elastase inhibition rate and compared with the blank control. The results are as follows:

[0115] Table 9

[0116]

[0117] DPPH free radical scavenging rate experiment: After diluting the essence milk into 50% emulsion with 95% ethanol, mix it with the DPPH free radical sample and let it stand at room temperature for 5 minutes. The absorbance value was measured by ultraviolet spectrophotometer for analysis. The DPPH free radical scavenging rate data of the essence milk was obtained and compared with the blank control. The results are as follows:

[0118] Table 10

[0119] Blank control Essence milk sample P-value Significance of difference Free radical scavenging rate, % 1.84±0.16 39.5±0.15 ＜0.05 Significant

[0120] Comparative test of the effect of the composition ratio of essence lotion on product efficacy:

[0121] Comparative Example 1:

[0122] The skin care composition of this comparative example is different from that of Example 2 in that:

[0123] The addition ratio of red scorpion toxin peptide, acetyl hexapeptide-8 and dipeptide diaminobutyryl benzyl amide in the skin care composition is 1:3:2, and the total weight components of the three ingredients remain unchanged.

[0124] Comparative Example 2:

[0125] The skin care composition of this comparative example is different from that of Example 2 in that:

[0126] The addition ratio of red scorpion toxin peptide, acetyl hexapeptide-8 and dipeptide diaminobutyryl benzyl amide in the skin care composition is 1:7:6; and the total weight components of the three ingredients remain unchanged.

[0127] Comparative Example 3:

[0128] The skin care composition of this comparative example is different from that of Example 2 in that:

[0129] The addition ratio of red scorpion toxin peptide, acetyl hexapeptide-8 and dipeptide diaminobutyryl benzyl amide in the skin care composition is 1:10:6. The addition amount of red scorpion toxin peptide remains unchanged, while the addition amounts of acetyl hexapeptide-8 and dipeptide diaminobutyryl benzyl amide increase accordingly, that is, the total addition amount of the three raw materials increases.

[0130] Comparative Example 4:

[0131] The skin care composition of this comparative example is different from that of Example 2 in that:

[0132] The ratio of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and celery extract in the skin care composition is 1:5:5:10:10; and the total weight composition of the five ingredients remains unchanged.

[0133] Comparative Example 5:

[0134] The skin care composition of this comparative example is different from that of Example 2 in that:

[0135] The ratio of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and celery extract in the skin care composition is 1:8:5:12:12; and the total weight composition of the five ingredients remains unchanged.

[0136] Comparative Example 6:

[0137] The skin care composition of this comparative example is different from that of Example 2 in that:

[0138] The ratio of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and celery extract in the skin care composition is 1:10:10:15:20; and the total weight composition of the five ingredients remains unchanged.

[0139] Comparative Example 7:

[0140] The skin care composition of this comparative example is different from that of Example 3 in that:

[0141] The ratio of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and celery extract in the skin care composition is 1:20:15:15:10; and the total weight composition of the five ingredients remains unchanged.

[0142] Comparative Example 8:

[0143] The skin care composition of this comparative example is different from that of Example 3 in that:

[0144] The ratio of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and immortal grass extract in the skin care composition is 1:20:20:25:30; the amount of ginseng tissue culture remains unchanged, and the other four components are increased accordingly, that is, the total anti-wrinkle raw materials are increased.

[0145] Comparative test results 1:

[0146] 10 people were recruited for product experience test in Example 2 and Comparative Examples 1-3 respectively. Before using the product, the face was cleaned with the same facial cleanser, and then the face was dried. The initial value of skin wrinkles before use was analyzed by taking photos with a VISIA detector. Then, according to the following usage method, it was used once in the morning and evening every day. After applying the same moisturizing water, essence milk sample, and the same moisturizing cream with only basic moisturizing effect for 1 hour, the skin wrinkles after use were tested again; the instant anti-wrinkle effect of the essence milk of Example 2 and Comparative Examples 1-3 were compared. Each group of values ​​takes the average value of the characteristic values ​​of 10 experiencers (the smaller the value, the better); and then the comparison results of the improvement effect of the product on skin wrinkles are obtained.

[0147] Table 11 Experimental test results of Example 2 and Comparative Examples 1-3

[0148] Average wrinkle count Initial Value Test value Change value Example 2 99.1 69.8 -29.3 Comparative Example 1 92.2 81.8 -10.4 Comparative Example 2 88.9 71.5 -17.4 Comparative Example 3 99.8 79 -20.8

[0149] It can be seen from the results of Table 8 that after using the samples of Example 2 of the present invention and Comparative Examples 1-3, the test values ​​of the number of skin wrinkle characteristics of the experiencers were all lower than the initial values; and the improvement effects of Example 2 were all higher than those of Comparative Examples 1-3; the three instant anti-wrinkle ingredients had significantly different anti-wrinkle effects at different proportions. Under the condition that the ratio of red scorpion toxin peptide, acetyl hexapeptide-8, and dipeptide diaminobutyryl benzylamide diacetate in Example 2 of the present invention was 1:8:3, the instant anti-wrinkle effect was the best. Comparative Examples 1-2 and Example 2 showed that the best effect was achieved under the optimal ratio when the total amount of anti-wrinkle peptides used remained the same. Even if the overall use of the instant anti-wrinkle peptides in Comparative Example 3 increased, the use effect was still not as good as that of Example 2 under the non-optimal addition ratio; therefore, it was shown that red scorpion toxin peptide, acetyl hexapeptide-8, and dipeptide diaminobutyryl benzylamide diacetate had obvious synergistic effects.

[0150] Comparative test results 2:

[0151] 10 people were recruited for product experience test in Example 2 and Comparative Examples 4-8 respectively, and the test was carried out according to the method of Experimental Example 2; the comparison results of the improvement effect on skin wrinkles are as follows: (each group of values ​​is the average value of the characteristic values ​​of 10 experiencers, and the smaller the value, the better)

[0152] Table 12 Experimental test results of Example 2 and Comparative Examples 4-8

[0153]

[0154]

[0155] As shown in Table 9, after using the samples of Example 2 and Comparative Examples 4-8 of the present invention, the test values ​​of the number of skin wrinkle characteristics of the experiencers were all lower than the initial values; and the improvement effects of Example 2 were all higher than those of Comparative Examples 4-8; the 5 long-acting anti-wrinkle ingredients had significantly different anti-wrinkle effects at different proportions. Under the condition that the ratio of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ektoin, and immortal grass extract in Example 2 of the present invention was 1:15:15:20:25, the immediate anti-wrinkle effect was the best. Comparative Examples 4-7 and Example 2 were compared and analyzed to show that the best effect was achieved under the optimal ratio conditions when the total amount of anti-wrinkle and firming ingredients used was the same. Even if the total amount of anti-wrinkle and firming ingredients used in Comparative Example 8 was increased, the effect was still not as good as that of Example 2 under the non-optimal addition ratio; therefore, it is shown that the combination of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ektoin, and immortal grass extract in the essence milk of the present invention has a significant synergistic effect.

[0156] The above is only a preferred embodiment of the present invention, and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technician familiar with the present invention can make some changes or modify the technical contents suggested above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the solution of the present invention.

Claims

1. An anti-wrinkle and firming micro-nano essence lotion, characterized by: The composition comprises the following components in parts by weight: 0.005-0.1 parts of ginseng tissue culture, 0.1-5 parts of PEG / PPG-70 / 30 tocopherol ether, 0.1-1 parts of hydrogenated lecithin, 3-12 parts of emollients, 3-15 parts of moisturizers, 0.0001-0.01 parts of red scorpion toxin peptide, 0.1-3 parts of ectoine, 0.05-2 parts of immortal grass extract, 0.01-2 parts of ergothioneine, 0.001-0.1 parts of acetyl hexapeptide-8, 0.001-0.1 parts of dipeptide diaminobutyryl benzylamide diacetate, 0.1-2 parts of hydrolyzed collagen, and 50-85 parts of deionized water; The mass ratio of PEG / PPG-70 / 30 tocopherol ether to hydrogenated lecithin is 1:(0.1-2).

2. The anti-wrinkle and firming micro-nano essence lotion according to claim 1, characterized in that: The mass ratio of PEG / PPG-70 / 30 tocopherol ether: hydrogenated lecithin is 1:0.

5.

3. The anti-wrinkle and firming micro-nano essence lotion according to claim 1, characterized in that: The particle size distribution range of the nano-microemulsion is: D50: 100-300nm, D90: 350-500nm.

4. The anti-wrinkle and firming micro-nano essence lotion according to claim 1, characterized in that: The mass ratio of red scorpion toxin peptide, acetyl hexapeptide-8, and dipeptide diaminobutyryl benzylamide diacetate is 1:(5-10):(2-5).

5. The anti-wrinkle and firming micro-nano essence lotion according to claim 4, characterized in that: The mass ratio of red scorpion toxin peptide, acetyl hexapeptide-8, and dipeptide diaminobutyryl benzylamide diacetate is 1:8:

3.

6. The anti-wrinkle and firming micro-nano essence lotion according to claim 1, characterized in that: The mass ratio of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and herbaceous vine extract is 1:(5-20):(5-20):(10-30):(10-30).

7. The anti-wrinkle and firming micro-nano essence lotion according to claim 6, characterized in that: The mass ratio of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and herbaceous vine extract is 1:15:15:20:

25.

8. The anti-wrinkle and firming micro-nano essence lotion according to any one of claims 1 to 7, characterized in that: The following ingredients are also included in parts by weight: Palmitoyl tetrapeptide-7 0.001-0.1 parts, palmitoyl pentapeptide-4 0.001-0.1 parts, palmitoyl tripeptide-5 0.001-0.1 parts, hexapeptide-9 0.001-0.1 parts.

9. The anti-wrinkle and firming micro-nano essence lotion according to any one of claims 1 to 7, characterized in that: The emollient grease comprises the following ingredients in parts by weight: 0.5-5 parts of squalane, 1-5 parts of isononyl isononanoate, 1-5 parts of polydimethylsiloxane, 0.01-1 parts of ubiquinone; The moisturizing agent comprises the following ingredients in parts by weight: 0.5-3 parts of betaine, 0.1-2 parts of panthenol, 0.1-3 parts of acetyl glucosamine, 0.01-1 parts of sodium hyaluronate, 0.01-1 parts of hydrolyzed sodium hyaluronate, 1-5 parts of 1,2-pentanediol, 2-6 parts of butanediol, and 0.1-3 parts of trehalose.

10. A method for preparing the anti-wrinkle and firming micro-nano essence lotion according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1 Water phase: add the moisturizer and the extract of Immortal Grass to 90% of deionized water, heat to 80-85°C, and stir to fully disperse; S2 oil phase: Stir and heat PEG / PPG-70 / 30 tocopherol ether, hydrogenated lecithin, and emollient oil to melt and disperse evenly, and heat the oil phase to 80-85°C and maintain; S3: Mix the oil phase and the water phase, start homogenization and stirring for 10-15 minutes, and maintain the temperature at 80-85°C; S4: After homogenization, cool to 45-40°C, add ginseng tissue culture, red scorpion toxin peptide, ectoine, ergothioneine, acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, and hydrolyzed collagen, stir evenly, and add the remaining 10% deionized water to 100 parts.

11. The preparation method according to claim 10, characterized in that: The following steps are involved: S1 aqueous phase: add butylene glycol, 1,2-pentanediol, trehalose, betaine, panthenol, acetyl glucosamine, celery extract, sodium hyaluronate, and hydrolyzed sodium hyaluronate to 90% of deionized water, heat to 80-85°C, and stir to fully disperse; S2 oil phase: Stir and heat PEG / PPG-70 / 30 tocopherol ether, hydrogenated lecithin, isononyl isononanoate, squalane, polydimethylsiloxane, and ubiquinone to melt and disperse evenly, and heat the oil phase to 80-85°C and maintain; S3: Mix the oil phase and the water phase, start homogenization and stirring for 10-15 minutes, and maintain the temperature at 80-85°C; S4: After homogenization, cool to 45-40°C, add ginseng tissue culture, red scorpion toxin peptide, ectoine, ergothioneine, acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, hydrolyzed collagen, palmitoyl tetrapeptide-7, palmitoyl pentapeptide-4, palmitoyl tripeptide-5, hexapeptide-9 and mix well, and add the remaining 10% deionized water to 100 parts.

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