A python oil compound for treating skin burns, scalds and cuts and a preparation method thereof

Abstract

The invention discloses a python oil compound for treating skin burns, scalds and cuts and a preparation method thereof, and relates to the technical field of traditional Chinese medicine. The python oil compound formula is composed of python oil extract, aloe, borneol, jade square gold tablets, psoralea corylifolia, scutellaria baicalensis, rosmarinus officinalis, sophora flavescens extract, cnidium monnieri and glycerol. Appropriate raw materials are weighed in parts by weight, and the python oil compound is obtained through screening, crushing, boiling and cooling. The python oil compound for treating skin burns, scalds and cuts provided by the invention has a reasonable ratio of Chinese medicinal materials, has strong permeability, can promote blood circulation, has obvious analgesic effect, can repair damaged cells, is not easy to leave scars after healing, and has the advantages of simple preparation process and convenient product use.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese herbal medicines, and in particular to a python oil compound for treating skin burns, scalds and cuts and a preparation method thereof. Background Art

[0002] Burns and scalds are common accidental injuries in life. Burns and scalds caused by boiling water, boiling porridge, hot oil, and hot steam are common. Burns and scalds are a complex surgical disease caused by the action of fire, boiling oil, fire, electricity, or acid-base substances on human skin. Mild burns or scalds cause redness, swelling, burning pain, or blisters on the epidermis; severe burns and scalds damage the skin and subcutaneous tissue, attack the internal organs, damage the yin and yang, and often lead to water and electrolyte imbalance in the human body, redness, swelling, ulceration, infection and other serious consequences of skin tissue. Cuts refer to the skin, subcutaneous tissue or deep tissue being cut by sharp objects such as glass slides and blades, resulting in damage and laceration. The wound is relatively neat and small in area, but there is more bleeding. The edge tissue of a few wounds is relatively rough due to fragmentation. In severe cases, muscles, nerves, large blood vessels, etc. may be cut, and even limbs may be severed. If the wound is not treated in time, it will not heal for a long time due to infection, resulting in scars and affecting limb function.

[0003] At present, the treatment of burns and scalds is generally made into ointments using antibiotic drugs extracted from Chinese herbal medicines or minerals, and then applied to the wound surface. Although the above ointments can produce certain therapeutic effects in treatment, most scald ointments are mainly for the treatment of acute burns, and have slow effects on swelling, pain relief, and tissue regeneration, poor permeability, and long healing time, and their healing effect is limited. At present, drugs for cuts generally use oral antibiotics such as amoxicillin capsules and cefixime granules. Such antibiotics are prone to the production of drug-resistant bacteria, causing adverse reactions in the human body, and are prone to causing side effects such as dual infection. Therefore, there is an urgent need for a python oil complex that is safe to use, highly permeable, has good healing effects and a short healing time, and can treat burns, scalds, and cuts at the same time. Summary of the invention

[0004] In view of the above-mentioned defects of the prior art, the present invention provides a python oil compound for treating skin burns, scalds and cuts and a preparation method thereof, so as to solve the problems raised by the above-mentioned background technology.

[0005] To achieve the above object, the present invention provides the following technical scheme: a python oil complex for treating skin burns, scalds and cuts, comprising the following ingredients: python oil extract (Python molurus bivittatus), aloe (a concentrated dry product of leaf juice of Aloe barbadensis Miller, a plant of the Liliaceae family), borneol (a crystal extracted from a resin processed product of the plant Dipterocarp Tree Dryobalanops aromatica Gaertn.f.), Yujin Fangpian (produced by Luoyang Xinchundu Biopharmaceutical Co., Ltd.), psoralea corylifolia (a dried mature fruit of the leguminous plant Psoralea corylifolia L.), hooked twigs (a hooked branch of the plant Ramulus Uncariae Cum Uncis), and rosenbergia lappa (a plant of the Apocynaceae family).

[0006] The ingredients include dried leafy vines of Trachelospermum jasminoides (Lindl.) Lem.), Sophora flavescens extract (extracted from the dried root of Sophorae Flavescentis Radix), Cnidium monnieri (dried mature fruit of Cnidium monnieri (L.) Cuss. of the Apiaceae family), and glycerin (produced by Croda Pharmaceuticals and Health).

[0007] Preferably, the python oil complex is composed of the following ingredients in parts by weight: 5-7 parts of python oil extract, 2-4 parts of aloe, 2-4 parts of borneol, 8-10 parts of Yujinfang tablets, 1-3 parts of psoralea corylifolia, 5-7 parts of Goutian chinensis, 4-6 parts of Caulis Rosmarinis, 1-3 parts of Sophora flavescens extract, 4-6 parts of Cnidium monnieri and 6-8 parts of glycerin.

[0008] Preferably, the python oil complex is composed of the following ingredients in parts by weight: 5 parts of python oil extract, 2 parts of aloe, 2 parts of borneol, 8 parts of Yujinfang tablets, 1 part of psoralea corylifolia, 5 parts of Goutian chinensis, 4 parts of Rosa rugosa, 1 part of Sophora flavescens extract, 4 parts of Cnidium monnieri and 6 parts of glycerin.

[0009] Preferably, the python oil complex is composed of the following ingredients in parts by weight: 7 parts of python oil extract, 4 parts of aloe, 4 parts of borneol, 10 parts of Yujinfang tablets, 3 parts of psoralea corylifolia, 7 parts of Goutian chinensis, 6 parts of Rosa rugosa, 3 parts of Sophora flavescens extract, 6 parts of Cnidium monnieri and 8 parts of glycerin.

[0010] Preferably, the python oil complex is composed of the following ingredients in parts by weight: 6 parts of python oil extract, 3 parts of aloe, 3 parts of borneol, 9 parts of Yujinfang tablets, 2 parts of psoralea corylifolia, 6 parts of Goutian chinensis, 5 parts of Rosa rugosa, 2 parts of Sophora flavescens extract, 5 parts of Cnidium monnieri and 7 parts of glycerin.

[0011] Furthermore, a preparation method of a python oil compound for treating skin burns, scalds and cuts is provided, wherein the preparation method is carried out according to the following steps:

[0012] S1. Raw material preparation: weigh the raw materials according to weight, and select, screen, and remove dust and impurities respectively;

[0013] S2, raw material grinding: Yujin Fangpian, Bu Gu Zhi, Gouteng, Luo Shi Caulis, Cnidium monnieri are mixed and put into a traditional Chinese medicine powder grinder for grinding, and Borneol is ground into powder and mixed with aloe vera, Sophora flavescens extract, and glycerin for later use;

[0014] S3, raw material soaking: put the raw materials described in S2 into python oil extract, soak for 8 to 10 hours, and obtain raw material soaking liquid;

[0015] S4, one-time boiling: the raw material soaking liquid described in S3 is first boiled with a slow fire, stirring continuously during the boiling process, and after boiling for 9 to 15 minutes, the residue is filtered to remove the residue, and the remaining liquid is the liquid medicine;

[0016] S5, secondary boiling: cool the liquid medicine obtained in S4 to 40-45°C, and then boil it over high heat for 6-8 minutes, stirring continuously during the boiling process;

[0017] S6. Cooling: Cool the liquid medicine obtained in S5 naturally in a dry and dust-free environment, and bottle it for later use after cooling.

[0018] Furthermore, the raw material soaking time in step S3 is 9 hours, the slow fire boiling time in step S4 is 12 minutes, and the high fire boiling time in step S5 is 7 minutes;

[0019] Further, the preparation method of python oil extract is as follows:

[0020] S1. First, perform a laparotomy on the python, remove the fat from its body, and clean it to remove impurities;

[0021] S2, removing the film on the surface of python fat by hand to obtain membrane-free python fat;

[0022] S3. Pour the membrane-free python fat obtained in S2 into a snake oil refining pot, adjust the temperature to maintain at 95±10° C., wait until all the snake oil is refined, filter to remove the oil residue, and obtain the python oil extract after cooling.

[0023] Furthermore, the preparation method of the Sophora flavescens extract is as follows:

[0024] Take the processed Sophora flavescens, add 85% ethanol to soak for 0.5h, and reflux extract 3 times at 80℃ on a digital constant temperature electric heating jacket, wherein the amount of ethanol added is 10 times the weight of Sophora flavescens, and the reflux extraction time is 2h. The collected extract is the Sophora flavescens extract.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows: the python oil complex for treating skin burns, scalds and cuts provided by the present invention has a reasonable ratio of medicinal materials, strong permeability, can promote blood circulation, has obvious analgesic effect, can repair damaged cells, is not easy to leave scars after healing, has a simple preparation process, and is easy to use; it can simultaneously target skin burns, scalds and cuts; the python oil complex prepared by the present invention has a strong inhibitory effect on Candida albicans, Pseudomonas aeruginosa and Staphylococcus aureus, and takes effect quickly (the inhibition rate can reach more than 97% 24 hours after contact with the bacteria); the python oil complex with the best ratio has a wound healing rate of 98% in the treatment of burns, scalds and cuts within 7 days. DETAILED DESCRIPTION

[0026] The technical solution of the present invention is further described in detail below in conjunction with specific implementation methods.

[0027] Example 1

[0028] The python oil complex for treating skin burns, scalds and cuts of the present embodiment is composed of the following ingredients in parts by weight: 5 parts of python oil extract, 2 parts of aloe, 2 parts of borneol, 8 parts of Yujinfang tablets, 1 part of psoralea corylifolia, 5 parts of Gastrodia elata, 4 parts of Caulis gentiana, 1 part of Sophora flavescens extract, 4 parts of Cnidium monnieri and 6 parts of glycerin.

[0029] The preparation method is carried out according to the following steps:

[0030] S1. Raw material preparation: python oil extract, aloe, borneol, Yujin Fangpian, psoralea corylifolia, gouting, rosenbergia lappa, sophora flavescens extract, cnidium monnieri and glycerin are weighed according to weight, and the raw materials are selected, screened, dusted and removed;

[0031] S2, raw material grinding: Yujin Fangpian, Bu Gu Zhi, Gouteng, Luo Shi Caulis, Cnidium monnieri are mixed and put into a traditional Chinese medicine powder grinder for grinding, and Borneol is ground into powder and mixed with aloe vera, Sophora flavescens extract, and glycerin for later use;

[0032] S3, raw material soaking: put the raw materials described in S2 into python oil extract, soak for 8 hours, and obtain raw material soaking liquid;

[0033] S4, one-time boiling: the raw material soaking liquid described in S3 is first boiled with a slow fire, stirring continuously during the boiling process, and after boiling for 9 minutes, the residue is filtered to remove the residue, and the remaining liquid is the liquid medicine;

[0034] S5, secondary boiling: cool the liquid medicine obtained in S4 to 40-45°C, and then boil it over high heat for 6 minutes, stirring continuously during the boiling process;

[0035] S6. Cooling: Cool the liquid medicine obtained in S5 naturally in a dry and dust-free environment, and bottle it for later use after cooling.

[0036] Example 2

[0037] The python oil complex of the present embodiment is for treating skin burns, scalds and cuts, and the python oil complex is composed of the following ingredients in parts by weight: 7 parts of python oil extract, 4 parts of aloe, 4 parts of borneol, 10 parts of Yujinfang tablets, 3 parts of psoralea corylifolia, 7 parts of Gastrodia elata, 6 parts of Caulis gentiana, 3 parts of Sophora flavescens extract, 6 parts of Cnidium monnieri and 8 parts of glycerin.

[0038] The preparation method is carried out according to the following steps:

[0039] S1. Raw material preparation: python oil extract, aloe, borneol, Yujin Fangpian, psoralea corylifolia, gouting, rosenbergia lappa, sophora flavescens extract, cnidium monnieri and glycerin are weighed according to weight, and the raw materials are selected, screened, dusted and removed;

[0040] S2, raw material grinding: Yujin Fangpian, Bu Gu Zhi, Gouteng, Luo Shi Caulis, Cnidium monnieri are mixed and put into a traditional Chinese medicine powder grinder for grinding, and Borneol is ground into powder and mixed with aloe vera, Sophora flavescens extract, and glycerin for later use;

[0041] S3, raw material soaking: put the raw materials described in S2 into python oil extract, soak for 10 hours, and obtain raw material soaking liquid;

[0042] S4, one-time boiling: the raw material soaking liquid described in S3 is first boiled with a slow fire for 15 minutes, stirring continuously during the boiling process, and filtering to remove the residue after the boiling time is reached, and the remaining liquid is the liquid medicine;

[0043] S5, secondary boiling: cool the liquid medicine obtained in S4 to 40-45°C, and then boil it over high heat for 8 minutes, stirring continuously during the boiling process;

[0044] S6. Cooling: Cool the liquid medicine obtained in S5 naturally in a dry and dust-free environment, and bottle it for later use after cooling.

[0045] Example 3

[0046] The python oil complex of the present embodiment is for treating skin burns, scalds and cuts, and the python oil complex is composed of the following ingredients in parts by weight: 6 parts of python oil extract, 3 parts of aloe, 3 parts of borneol, 9 parts of Yujinfang tablets, 2 parts of psoralea corylifolia, 6 parts of Gastrodia elata, 5 parts of Caulis gentiana, 2 parts of Sophora flavescens extract, 5 parts of Cnidium monnieri and 7 parts of glycerin.

[0047] The preparation method is carried out according to the following steps:

[0048] S1. Raw material preparation: python oil extract, aloe, borneol, Yujin Fangpian, psoralea corylifolia, gouting, rosenbergia lappa, sophora flavescens extract, cnidium monnieri and glycerin are weighed according to weight, and the raw materials are selected, screened, dusted and removed;

[0049] S2, raw material grinding: Yujin Fangpian, Bu Gu Zhi, Gouteng, Luo Shi Caulis, Cnidium monnieri are mixed and put into a traditional Chinese medicine powder grinder for grinding, and Borneol is ground into powder and mixed with aloe vera, Sophora flavescens extract, and glycerin for later use;

[0050] S3, raw material soaking: put the raw materials described in S2 into python oil extract, soak for 9 hours, and obtain raw material soaking liquid;

[0051] S4, one-time boiling: the raw material soaking liquid described in S3 is first boiled with a slow fire for 12 minutes, stirring continuously during the boiling process, and filtering to remove the residue after the boiling time is reached, and the remaining liquid is the liquid medicine;

[0052] S5, secondary boiling: cool the liquid obtained in S4 to 40-45°C, and then boil it over high heat for 7 minutes, stirring continuously during the boiling process;

[0053] S6. Cooling: Cool the liquid medicine obtained in S5 naturally in a dry and dust-free environment, and bottle it for later use after cooling.

[0054] Comparative Example 1

[0055] This comparative example differs from Example 3 in that no python oil is added, and the other components and contents remain unchanged.

[0056] The comparative example discloses a compound for treating skin burns, scalds and cuts, which is composed of the following ingredients in parts by weight: 3 parts of aloe, 3 parts of borneol, 9 parts of Yujinfang tablets, 2 parts of psoralea corylifolia, 6 parts of Gastrodia elata, 5 parts of Caulis Rosenbergiae, 2 parts of Sophora flavescens extract, 5 parts of Cnidium monnieri and 7 parts of glycerol.

[0057] The preparation method is carried out according to the following steps:

[0058] S1. Raw material preparation: weigh aloe vera, borneol, Yujin Fangpian, psoralea corylifolia, gouting, rosenbergia lappa, sophora flavescens extract, cnidium monnieri and glycerin according to weight, select, screen, remove dust and impurities respectively;

[0059] S2, raw material grinding: Yujin Fangpian, Bu Gu Zhi, Gouteng, Luo Shi Caulis, Cnidium monnieri are mixed and put into a traditional Chinese medicine powder grinder for grinding, and Borneol is ground into powder and mixed with aloe vera, Sophora flavescens extract, and glycerin for later use;

[0060] S3, one-time boiling: crush the raw materials described in S2, boil them with a slow fire for 12 minutes, stir them continuously during the boiling process, filter and remove the residue after the boiling time, and the remaining liquid is the liquid medicine;

[0061] S5, secondary boiling: cool the liquid obtained in S4 to 40-45°C, and then boil it over high heat for 7 minutes, stirring continuously during the boiling process;

[0062] S6. Cooling: Cool the liquid medicine obtained in S5 naturally in a dry and dust-free environment, and bottle it for later use after cooling.

[0063] Comparative Example 2

[0064] This comparative example differs from Example 3 in that no jade gold square tablets are added, and the other components and their contents remain unchanged.

[0065] The python oil complex of the present embodiment is for treating skin burns, scalds and cuts, and the python oil complex is composed of the following ingredients in parts by weight: 6 parts of python oil extract, 3 parts of aloe, 3 parts of borneol, 2 parts of psoralea corylifolia, 6 parts of Gynura strychnifolia, 5 parts of Caulis Rosenbergii, 2 parts of Sophora flavescens extract, 5 parts of Cnidium monnieri and 7 parts of glycerin.

[0066] The preparation method is carried out according to the following steps:

[0067] S1. Raw material preparation: weigh the raw materials according to weight, and select, screen, remove dust and impurities respectively, including python oil extract, aloe vera, borneol, psoralea corylifolia, galangal, rosa centella asiatica, sophora flavescens extract, cnidium monnieri and glycerin;

[0068] S2, raw material grinding: mix psoralea corylifolia, galangal, scutellaria baicalensis, and cnidium monnieri, put them into a traditional Chinese medicine powder grinder, grind borneol into powder, mix with aloe vera, sophora flavescens extract, and glycerin for later use;

[0069] S3, raw material soaking: put the raw materials described in S2 into python oil extract, soak for 9 hours, and obtain raw material soaking liquid;

[0070] S4, one-time boiling: the raw material soaking liquid described in S3 is first boiled with a slow fire for 12 minutes, stirring continuously during the boiling process, and filtering to remove the residue after the boiling time is reached, and the remaining liquid is the liquid medicine;

[0071] S5, secondary boiling: cool the liquid obtained in S4 to 40-45°C, and then boil it over high heat for 7 minutes, stirring continuously during the boiling process;

[0072] S6. Cooling: Cool the liquid medicine obtained in S5 naturally in a dry and dust-free environment, and bottle it for later use after cooling.

[0073] Comparative Example 3

[0074] This comparative example differs from Example 3 in that no Psoralea corylifolia is added, and the other components and their contents remain unchanged.

[0075] The python oil complex of the present embodiment is for treating skin burns, scalds and cuts, and the python oil complex is composed of the following ingredients in parts by weight: 6 parts of python oil extract, 3 parts of aloe, 3 parts of borneol, 9 parts of Yujin square tablets, 6 parts of Goutian ternata, 5 parts of Rosa rugosa, 2 parts of Sophora flavescens extract, 5 parts of Cnidium monnieri and 7 parts of glycerin.

[0076] The preparation method is carried out according to the following steps:

[0077] S1. Raw material preparation: weigh the raw materials according to weight, and select, screen, remove dust and impurities respectively, including python oil extract, aloe vera, borneol, Yujin Fangpian, Gouting, Rosa rugosa, Sophora flavescens extract, Cnidium monnieri and glycerin;

[0078] S2, raw material grinding: Yujin Fangpian, Gouting, Rosa rugosae and Cnidium monnieri are mixed and put into a traditional Chinese medicine powder grinder for grinding, and Borneol is ground into powder separately and then mixed with aloe vera, Sophora flavescens extract and glycerin for later use;

[0079] S3, raw material soaking: put the raw materials described in S2 into python oil extract, soak for 9 hours, and obtain raw material soaking liquid;

[0080] S4, one-time boiling: the raw material soaking liquid described in S3 is first boiled with a slow fire for 12 minutes, stirring continuously during the boiling process, and filtering to remove the residue after the boiling time is reached, and the remaining liquid is the liquid medicine;

[0081] S5, secondary boiling: cool the liquid obtained in S4 to 40-45°C, and then boil it over high heat for 7 minutes, stirring continuously during the boiling process;

[0082] S6. Cooling: Cool the liquid medicine obtained in S5 naturally in a dry and dust-free environment, and bottle it for later use after cooling.

[0083] Comparative Example 4

[0084] This comparative example differs from Example 3 in that no diced starch is added, and the other components and contents remain unchanged.

[0085] The python oil complex of the present embodiment is for treating skin burns, scalds and cuts, and the python oil complex is composed of the following ingredients in parts by weight: 6 parts of python oil extract, 3 parts of aloe, 3 parts of borneol, 9 parts of Yujinfang tablets, 2 parts of psoralea corylifolia, 5 parts of Rhizoma Cynomorii, 2 parts of Sophora flavescens extract, 5 parts of Fructus Cnidium monnieri and 7 parts of glycerol.

[0086] The preparation method is carried out according to the following steps:

[0087] S1. Raw material preparation: python oil extract, aloe, borneol, Yujin Fangpian, psoralea corylifolia, rosenbergia lappa, sophora flavescens extract, cnidium monnieri and glycerin are weighed according to weight, and the raw materials are selected, screened, dusted and removed;

[0088] S2, raw material grinding: Yujin Fangpian, Psoralea corylifolia, Caulis rosenbergii and Fructus cnidii are mixed and put into a traditional Chinese medicine powder grinder for grinding, and Borneol is ground into powder separately and then mixed with aloe vera, Sophora flavescens extract and glycerin for later use;

[0089] S3, raw material soaking: put the raw materials described in S2 into python oil extract, soak for 9 hours, and obtain raw material soaking liquid;

[0090] S4, one-time boiling: the raw material soaking liquid described in S3 is first boiled with a slow fire for 12 minutes, stirring continuously during the boiling process, and filtering to remove the residue after the boiling time is reached, and the remaining liquid is the liquid medicine;

[0091] S5, secondary boiling: cool the liquid obtained in S4 to 40-45°C, and then boil it over high heat for 7 minutes, stirring continuously during the boiling process;

[0092] S6. Cooling: Cool the liquid medicine obtained in S5 naturally in a dry and dust-free environment, and bottle it for later use after cooling.

[0093] Comparative Example 5

[0094] This comparative example is different from Example 3 in that: Roseburia schrenkiana is not added, and the other components and their contents remain unchanged.

[0095] The python oil complex of the present embodiment is for treating skin burns, scalds and cuts, and the python oil complex is composed of the following ingredients in parts by weight: 6 parts of python oil extract, 3 parts of aloe, 3 parts of borneol, 9 parts of Yujinfang tablets, 2 parts of psoralea corylifolia, 6 parts of Gouteng, 2 parts of Sophora flavescens extract, 5 parts of Cnidium monnieri and 7 parts of glycerin.

[0096] The preparation method is carried out according to the following steps:

[0097] S1. Raw material preparation: python oil extract, aloe, borneol, Yujin Fangpian, psoralea corylifolia, gouting, sophora flavescens extract, cnidium monnieri and glycerin are weighed according to weight, and the raw materials are selected, screened, dusted and removed;

[0098] S2, raw material crushing: Yujin Fangpian, Bu Gu Zhi, Gouting, Cnidium monnieri are mixed and put into a traditional Chinese medicine powder grinder for crushing, and Borneol is ground into powder separately and then mixed with aloe vera, Sophora flavescens extract, and glycerin for later use;

[0099] S3, raw material soaking: put the raw materials described in S2 into python oil extract, soak for 9 hours, and obtain raw material soaking liquid;

[0100] S4, one-time boiling: the raw material soaking liquid described in S3 is first boiled with a slow fire for 12 minutes, stirring continuously during the boiling process, and filtering to remove the residue after the boiling time is reached, and the remaining liquid is the liquid medicine;

[0101] S5, secondary boiling: cool the liquid obtained in S4 to 40-45°C, and then boil it over high heat for 7 minutes, stirring continuously during the boiling process;

[0102] S6. Cooling: Cool the liquid medicine obtained in S5 naturally in a dry and dust-free environment, and bottle it for later use after cooling.

[0103] Test Example 1 24h Antibacterial Ability Evaluation Test

[0104] 1.1 Trial Drugs

[0105] The python oil complexes prepared in Examples 1 to 3 and Comparative Examples 1 to 5 were used as test drugs.

[0106] 1.2 Test subjects

[0107] Candida albicans, Pseudomonas aeruginosa and Staphylococcus aureus were isolated and purified from the laboratory.

[0108] 1.3 Test methods

[0109] The 24h contact antibacterial ability evaluation test of the python oil compound was conducted according to the national standard GB / T 16886.5-2003.

[0110] Inhibition rate = colony diameter (CK) - colony diameter (treatment) / colony diameter (CK)

[0111] 1.4 Test results

[0112] Table 1 Antibacterial rate after 24h contact

[0113]

[0114] The results of test 1 show that the inhibitory effect of the python oil complex on Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus is more than 97%, proving that the active ingredients in the python oil complex have antibacterial ability. By comparing Example 3 with Comparative Example 1, the inhibitory effect of Comparative Example 1 on Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus is less than 55%, indicating that the addition of python oil to the formula of the present application is very important for the efficacy of the complex.

[0115] Test Example 2 Burn Wound Healing Effect

[0116] 2.1 Trial Drugs

[0117] The python oil complexes prepared in Examples 1 to 3 and Comparative Examples 1 to 5 were used as test drugs.

[0118] 2.2 Test subjects

[0119] 100 male Wistar rats were used as experimental animals, with 10 rats in each group.

[0120] 2.3 Test methods

[0121] The back of male Wistar rats was depilated with 8% sodium sulfide solution, and 1-2% fentanyl mixture was injected into the local part of the mouse muscle for anesthesia, and then scalded with 85℃ water bath for 15 seconds, causing 10% deep II degree skin burns on the back of the rats. After the injury, 5mL of normal saline was injected into the abdominal cavity, and the python oil complex was directly applied to the affected area three times a day. Ten of the rats were injected with 5mL of normal saline into the abdominal cavity after the scald, and then no treatment was used as the blank group; the other 10 rats were injected with 5mL of normal saline into the abdominal cavity after the scald, and commercially available burn and scald ointment (main ingredients: 30g of raw rhubarb powder, 60g of sanguisorba officinalis powder, 500ml of sesame oil, 60g of yellow wax, 192g of badger oil, 28.8g of sanguisorba officinalis, 6g of borneol, and 12g of white vaseline) was directly applied to the affected area three times a day, which was used as the positive control group; the observation points were selected at 3, 7, 10, and 14 days after the injury to calculate the wound healing rate.

[0122] Wound healing rate = (original wound area - unhealed wound area) / original wound area.

[0123] 2.4 Test results

[0124] Table 2 Burn wound healing rate

[0125]

[0126] The results of Experiment 2 show that adding the python oil complex with the best ratio has the highest wound healing rate and the shortest time for burns, scalds and cuts; by comparing Example 3 with Comparative Examples 1 to 5, the absence of python oil in the complex leads to a low healing rate of burn wounds and a long treatment period; the addition of Yujin Fangpian, Psoralea corylifolia, Gouting and Rosa rugosa to the python oil complex can promote the absorption of drugs by the skin and shorten the healing time; the results of the blank group and the positive control group show that if no treatment measures are taken after the burn, it will only lead to wound deterioration. The python oil complex prepared in Example 3 is better than the burn ointment in treating burns and scalds, and has a shorter healing time.

[0127] Experimental Example 3: Healing Effect of Cutting Wounds

[0128] 3.1 Trial Drugs

[0129] The python oil complex prepared in Example 3 was used as a test drug.

[0130] 3.2 Test subjects

[0131] Thirty male Wistar rats were used as experimental animals, with 10 rats in each group.

[0132] 3.3 Test methods

[0133] The back of anesthetized male Wistar rats was cut with a scissor knife to form a wound with a length of 1 to 2 cm and a depth of 4 to 5 cm. After the injury, 5 mL of normal saline was injected into the abdominal cavity, and the python oil complex prepared in Example 3 was directly applied to the affected area three times a day. Ten of the rats were injected with 5 mL of normal saline into the abdominal cavity after scalding, and then no treatment was performed as a blank group; the other 10 rats were injected with 5 mL of normal saline into the abdominal cavity after scalding, and commercially available fusidic acid cream (main ingredients: 10 g of hexadecanol, 12 g of glycerol, 10 g of liquid paraffin, 22 g of polysorbate, 15 g of potassium sorbate, 5 g of butylated hydroxyanisole, and 30 g of pure water) was directly applied to the affected area three times a day, and this treatment was used as a positive control; observation points were selected at 3, 7, 10, and 14 days after the injury to calculate the wound healing rate.

[0134] Wound healing rate = (original wound area - unhealed wound area) / original wound area.

[0135] 3.4 Test results

[0136] Table 3 Cutting wound healing rate

[0137]

[0138] The results of Experiment 3 show that the python oil complex prepared in the optimal ratio Example 3 has a good healing effect not only on burns and scalds, but also on cuts; the results of the blank group and the positive control group show that if no treatment measures are taken after scalding, the wound surface will only increase and the healing rate will decrease. The python oil complex prepared in Example 3 is better than fusidic acid cream in treating cuts, and the fusidic acid cream has a long healing period for cuts.

[0139] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A python oil compound for treating skin burns, scalds and cuts, characterized in that: The invention is prepared from the following ingredients in parts by weight: 5-7 parts of python oil extract, 2-4 parts of aloe vera, 2-4 parts of borneol, 8-10 parts of Yujin Fangpian, 1-3 parts of psoralea corylifolia, 5-7 parts of gouting, 4-6 parts of rosenbergia lappa, 1-3 parts of sophora flavescens extract, 4-6 parts of cnidium monnieri and 6-8 parts of glycerin; The preparation method of the python oil extract is as follows: S1. First, perform a laparotomy on the python, remove the fat from its body, and clean it to remove impurities; S2, removing the film on the surface of python fat by hand to obtain membrane-free python fat; S3, pouring the membrane-free python fat obtained in S2 into a snake oil refining pot, adjusting the temperature to maintain at 95±10° C., until all the snake oil is refined, filtering to remove the oil residue, and cooling to obtain the python oil extract; The trachelospermum jasminoides is the dried leafy vine stem of the plant Trachelospermum jasminoides (Lindl.) Lem. of the Apocynaceae family; The preparation method of the Sophora flavescens extract is as follows: Take the processed Sophora flavescens, add 85% ethanol to soak for 0.5h, and reflux extract 3 times at 80℃ on a digital constant temperature electric heating jacket, wherein the amount of ethanol added is 10 times the weight of Sophora flavescens, and the reflux extraction time is 2h. The collected extract is the Sophora flavescens extract.

2. The python oil compound for treating skin burns, scalds and cuts according to claim 1, characterized in that: The invention is prepared from the following ingredients in parts by weight: 5 parts of python oil extract, 2 parts of aloe vera, 2 parts of borneol, 8 parts of Yujin Fang tablets, 1 part of psoralea corylifolia, 5 parts of Goutian chinensis, 4 parts of Caulis Rosmarinis, 1 part of Sophora flavescens extract, 4 parts of Cnidium monnieri and 6 parts of glycerol.

3. The python oil compound for treating skin burns, scalds and cuts according to claim 1, characterized in that: The invention is prepared from the following ingredients in parts by weight: 7 parts of python oil extract, 4 parts of aloe vera, 4 parts of borneol, 10 parts of Yujinfang tablets, 3 parts of psoralea corylifolia, 7 parts of Goutian chinensis, 6 parts of Rosa rugosa vine, 3 parts of Sophora flavescens extract, 6 parts of Cnidium monnieri and 8 parts of glycerol.

4. The python oil compound for treating skin burns, scalds and cuts according to claim 1, characterized in that: The invention is prepared from the following ingredients in parts by weight: 6 parts of python oil extract, 3 parts of aloe, 3 parts of borneol, 9 parts of Yujinfang tablets, 2 parts of psoralea corylifolia, 6 parts of Goutian chinensis, 5 parts of Rosa rugosa vine, 2 parts of Sophora flavescens extract, 5 parts of Cnidium monnieri and 7 parts of glycerol.

5. The method for preparing a python oil compound for treating skin burns, scalds and cuts according to any one of claims 1 to 4, characterized in that: The preparation method is carried out according to the following steps: S1. Raw material preparation: weigh the raw materials according to weight, and select, screen, and remove dust and impurities respectively; S2, raw material grinding: Yujin Fangpian, Bu Gu Zhi, Gouteng, Luo Shi Caulis, Cnidium monnieri are mixed and then ground, Borneol is ground into powder and then mixed with aloe vera, Sophora flavescens extract, and glycerin for later use; S3, raw material soaking: put the raw materials described in S2 into python oil extract, soak for 8 to 10 hours, and obtain raw material soaking liquid; S4, one-time boiling: the raw material soaking liquid described in S3 is first boiled with a slow fire, stirring continuously during the boiling process, and after boiling for 9 to 15 minutes, the residue is filtered to remove the residue, and the remaining liquid is the liquid medicine; S5, secondary boiling: after cooling the liquid medicine obtained in S4, boil it again with high fire for 6-8 minutes, stirring continuously during boiling; S6. Cooling: Cool the liquid medicine obtained in S5 naturally in a dry and dust-free environment, and bottle it for later use after cooling.

6. The method for preparing a python oil compound for treating skin burns, scalds and cuts according to claim 5, characterized in that: The raw material soaking time in step S3 is 9 hours, the slow fire boiling time in step S4 is 12 minutes, and the high fire boiling time in step S5 is 7 minutes.

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