Preparation and application of a probiotic fermented composite plant extract

Abstract

The present invention provides a preparation and application of a probiotic fermented composite plant preservative, the preservative composition comprising the following components: probiotic jujube bark fermentation liquid, yeast peony root bark fermentation liquid, synergistic enhancer and purified water; in addition, the present invention also discloses probiotic jujube bark fermentation liquid and yeast peony root bark fermentation liquid, and adds them to skin care products that need preservation or anti-oxidation, such as essence, lotion, cream, sunscreen and facial mask. Experiments have shown that the skin care preservative for inhibiting microbial growth provided by the present invention has significant efficacy. Through the antiseptic challenge test of skin care products, the product using the composition is added, and the antiseptic ability is significantly improved, and it also has anti-oxidation skin care effect; the preservative composition of the present invention is natural in origin, has good antibacterial effect, is safe and mild, and is easy to use.

Description

Technical Field

[0001] The invention relates to a composite plant fermentation liquid with antiseptic effect and a preparation method and application thereof, belonging to the technical field of skin care products. Background Art

[0002] Cosmetics are easily contaminated by microorganisms. The main reason is that most of the effective content of cosmetics is water, and they also contain some oils, nutrients and active ingredients. In addition, the pH of general cosmetics is 4-7, which can provide a good environment for the growth of microorganisms. Once a product is contaminated by microorganisms, it is easy to spoil. Mold spots caused by mold and yeast will appear on the edges of product packaging. Products contaminated by microorganisms will become turbid, precipitate, change color, change pH, foam and change taste. If it is an emulsion, it may break and form lumps. What's more frightening is that some microorganisms will also produce some toxic substances, causing skin allergies in users, and even skin infections. In severe cases, it can cause sepsis. Preservatives refer to substances that can prevent the growth of microorganisms.

[0003] In cosmetics, the role of preservatives is to protect products from microbial contamination and extend the shelf life of products; to ensure product safety and prevent consumers from possible infection caused by using products contaminated by microorganisms. If the amount of preservatives added is insufficient, microorganisms may adapt to the surrounding growth environment and develop drug resistance, thus causing the preservative to fail.

[0004] With the in-depth study of the toxicology and safety of cosmetic preservatives, the adverse effects of some preservatives have gradually attracted attention. Preservatives are the second largest cause of cosmetic contact dermatitis, second only to fragrances. Formaldehyde releasers, hydroxyphenyl esters, methylchloroisothiazolone / methylisothiazolinone, methyldibromoglutaronitrile, etc. have been found to cause allergic contact dermatitis in allergic people. Some preservatives also have certain carcinogenic risks or other health hazards. Therefore, as people pay more and more attention to skin safety, alternative preservative products that use "fence technology" more gently are increasingly favored by the market, that is, without adding traditional preservatives, but through a reasonable combination of various microbial inhibitory factors, such as adding multifunctional raw materials with microbial growth inhibitory activity, reducing the water activity of the formula, adjusting the pH value, strictly following the principles of good manufacturing quality management practices (GMP), and selecting appropriate packaging materials, the irritation of the formula is greatly reduced while effectively inhibiting the growth of microorganisms, meeting the needs of consumers for mild, green and safe cosmetics. The alternative preservative theory and technology using "fence technology" has gradually improved and become an industry development trend.

[0005] Although the irritation of preservatives to the skin can be appropriately reduced through reasonable combinations, there has been no research on the natural sources of preservatives. Therefore, it is necessary to develop a preservative obtained by fermenting natural plants with probiotics.

[0006] Zizyphus joazeiro, also known as orange tree, belongs to the family Rhamnaceae of the order Rosales. Zizyphus joazeiro bark is rich in compounds such as betulinic acid, caffeine, saponins, triterpenes, mineral salts (calcium, iron, phosphorus, magnesium, potassium), vitamin C, and other compounds. It contains a variety of active ingredients such as flavonoids and tannins, which are mostly used for decoction and oral administration to treat diarrhea, and for external application to heal burns. In traditional methods of extracting Zizyphus joazeiro bark, alcohol extraction is often used, so there is a risk of reagent residues, which may cause irritation when used in skin care products. Therefore, modern technology and processes can be used to process Zizyphus joazeiro bark to maximize its application value.

[0007] Peony root bark is rich in paeonol, which has bactericidal, analgesic, anti-inflammatory, antipyretic and anti-allergic effects. Peony root bark extract is usually extracted with acetone, ethanol, etc. After extraction, organic solvents such as acetone and ethanol must be removed as much as possible. The residual solvent is not conducive to the use of the extract in cosmetics, and may make the operation steps dangerous and cumbersome. Therefore, it is meaningful to use a more natural and safe extraction method to obtain peony root bark extract. Summary of the invention

[0008] The purpose of the present invention is to provide a probiotic fermented plant extract, which has the effect of inhibiting the growth of microorganisms, and has a good inhibitory effect on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Aspergillus niger at a relatively low concentration. At the same time, the inventor creatively discovered that the extract also has a certain antioxidant effect. The main active ingredients in the composition are of natural origin, safe, mild and skin-friendly.

[0009] The object of the present invention is achieved through the following technical solutions:

[0010] A probiotic fermentation composite plant extract comprises the following components: lactic acid bacteria jujube bark fermentation liquid, yeast peony root bark fermentation liquid, a synergistic enhancer, and the balance is purified water.

[0011] Furthermore, the composite plant extract comprises 51-61% of probiotic Ziziphus jojoba bark fermentation liquid, 23-33% of yeast Paeonia suffruticosa root bark fermentation liquid, 16% of synergistic enhancer, and the balance is water.

[0012] Furthermore, the synergist is one or two of caprylyl glycol, pentylene glycol, propylene glycol and hexylene glycol.

[0013] Furthermore, the synergist is propylene glycol.

[0014] Fermentation broth usually has stability issues. In the present application, a certain amount of propylene glycol is added to solubilize and stabilize at low temperatures, thereby maintaining the stability of the components in the composition and improving the stability of the fermentation composition.

[0015] The preparation method of the probiotic jujube bark fermentation liquid comprises the following steps:

[0016] (1) Wash the bark of the jujube tree with clean water, dry it at 60°C, crush it and pass it through a sieve with a pore size of 0.21 mm for later use, and sterilize it by irradiation;

[0017] (2) anaerobically culturing lactic acid bacteria using MRS medium at 37° C.; adding irradiated Ziziphus jojoba bark powder in an amount of 5-12% by volume to the lactic acid bacteria culture solution and anaerobically fermenting for 48 hours to obtain a fermentation solution;

[0018] (3) The fermentation liquid is subjected to solid-liquid centrifugation separation, and the supernatant is the probiotic jujube bark fermentation liquid.

[0019] Preferably, the lactobacillus is one of Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus gasseri and Lactobacillus helveticus; the bark of Zizyphus joazeiro is dried or newly harvested bark of Zizyphus joazeiro; the irradiation sterilization is cobalt 60 irradiation or plasma beam irradiation sterilization; the irradiation intensity is 5000-8000 kGy; the centrifugal speed is 6000-12000 r / min,

[0020] Preferably, the lactobacillus is Lactobacillus rhamnosus, the jujube tree bark is dried jujube tree bark, the irradiation sterilization is ion beam irradiation sterilization, the irradiation intensity is 7000 kGy, the fermentation time is 60 hours, and the centrifugal speed is 10000 r / min.

[0021] The method for preparing the yeast peony root bark fermentation liquid comprises the following preparation steps:

[0022] (1) cleaning and crushing the root bark of Paeonia suffruticosa, drying it into powder, and sterilizing it by irradiation;

[0023] (2) culturing the yeast using potato culture medium so that the live yeast content in the culture medium is ≥106 cfu / ml;

[0024] (3) adding irradiated peony root bark powder in an amount of 2-10% by mass to the yeast culture solution, fermenting for 48-72 hours to obtain a fermentation solution; centrifuging the fermentation solution to obtain a supernatant; centrifuging the supernatant again to obtain a final peony root bark fermentation solution.

[0025] Preferably, the peony root bark is dried or newly picked peony root bark; the irradiation sterilization is cobalt 60 irradiation or plasma beam irradiation sterilization; the irradiation intensity is 5000-8000 kGy; and the centrifugal speed is 6000-12000 r / min.

[0026] Preferably, the peony root bark is dried peony root bark; the irradiation sterilization is plasma beam irradiation sterilization; the irradiation intensity is 7000 kGy, the fermentation time is 60 h; and the centrifugal speed is 10000 r / min.

[0027] The second object of the present invention is to provide the use of the above-mentioned composite plant extract in skin care products.

[0028] The skin care products include those with preservation and / or antioxidant requirements, such as lotions, emulsions, creams, sunscreens and facial masks.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] (1) The present invention provides a probiotic fermentation composite plant extract, which is an aqueous solution. The raw materials are from natural sources, no organic solvent needs to be added, no solvent residue is left after extraction, and it is safe, mild and easy to use.

[0031] (2) The addition of the synergist (propylene glycol) in the present invention can improve the stability of the fermentation composition, maintain the stability of the components in the composition, and enable the composition to more fully exert its efficacy.

[0032] (3) The composite plant extract provided by the present invention has a significant effect of inhibiting the growth of microorganisms and can be added to skin care products. On the basis of not adopting traditional preservation methods, it can effectively extend the shelf life of skin care products and ensure product stability and safety.

[0033] (4) The present invention utilizes lactic acid bacteria and yeast to ferment the bark of Ziziphus jojoba and the root bark of Paeonia suffruticosa respectively, which can release the effective active ingredients better and increase the content of betulinic acid, caffeine, saponin and brass components. Compared with a single extraction method, the method of the present invention can retain the plant active ingredients in all directions. The composite plant extract not only has a stronger antibacterial effect, but also has a stronger antioxidant effect, meeting the public's growing antioxidant needs.

[0034] (5) The present invention unexpectedly discovered that adding a composite plant extract to a sunscreen lotion can alleviate the irritation caused by the sunscreen to the skin, while achieving the effects of preservation and soothing, thus providing a new idea for soothing cosmetics.

[0035] (6) The lactic acid bacteria used in the present invention is a general term for a class of Gram-positive bacteria that are non-motile, non-spore-forming, and can utilize carbohydrates for fermentation and produce a large amount of lactic acid. They are widely found in the digestive tract, vagina, and oral cavity of humans and animals, and their species mainly include Lactobacillus, Bifidobacterium, and Streptococcus. Lactic acid bacteria are one of the most important probiotics. According to the principle of competition among microorganisms, the products fermented by them are more beneficial to the microecology of human skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] The present invention will be further described below in conjunction with the accompanying drawings.

[0037] Figure 1 Example 5: Cytotoxicity changes of different concentrations of the composition

[0038] Figure 2 ; Comparative Example 1 Cytotoxicity Changes of Compositions at Different Concentrations

[0039] Figure 3 ; Comparative Example 2 Cytotoxicity Changes of Compositions at Different Concentrations

[0040] Figure 4 ; Cytotoxicity changes of different concentrations of Ziziphus jojoba bark extract

[0041] Figure 5 ; Changes in cytotoxicity of different concentrations of peony root bark extract DETAILED DESCRIPTION

[0042] In order to better understand the present invention, the content of the present invention is further explained below in conjunction with examples, but the content of the present invention is not limited to the following examples, and the examples should not be regarded as limiting the scope of protection of the present invention.

[0043] Example 1

[0044] The preparation method of probiotic jujube bark fermentation liquid comprises the following steps:

[0045] (1) Wash the bark of the jujube tree with clean water, dry it at 60°C, crush it and pass it through a sieve with a pore size of 0.21 mm for later use, and sterilize it by irradiation;

[0046] (2) anaerobically culturing lactic acid bacteria using MRS medium at 37° C.; adding irradiated Ziziphus jojoba bark powder in an amount of 5-12% by volume to the lactic acid bacteria culture solution and anaerobically fermenting for 48 hours to obtain a fermentation solution;

[0047] (3) The fermentation liquid is subjected to solid-liquid centrifugation separation, and the supernatant is the probiotic jujube bark fermentation liquid.

[0048] In the above preparation method (2), the lactobacillus is one of Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus gasseri and Lactobacillus helveticus, preferably Lactobacillus rhamnosus.

[0049] The jujube bark is dried or newly harvested jujube bark, preferably dried jujube bark; the irradiation sterilization is cobalt 60 irradiation, plasma beam irradiation sterilization, preferably plasma beam irradiation sterilization; the irradiation intensity is 5000-8000 kGy, preferably 7000 kGy. The fermentation time is preferably 60 hours. In the centrifugation, the rotation speed is 6000-12000 r / min, preferably 10000 r / min.

[0050] Example 2

[0051] The preparation method of yeast peony root bark fermentation liquid comprises the following steps:

[0052] (1) cleaning and crushing the root bark of Paeonia suffruticosa, drying it into powder, and sterilizing it by irradiation;

[0053] (2) culturing the yeast using potato culture medium so that the live yeast content in the culture medium is ≥106 cfu / ml;

[0054] (3) adding irradiated peony root bark powder in an amount of 2-10% by mass to the yeast culture solution, fermenting for 48-72 hours to obtain a fermentation solution; centrifuging the fermentation solution to obtain a supernatant; centrifuging the supernatant again to obtain a final peony root bark fermentation solution.

[0055] The peony root bark is dried or newly harvested peony root bark, preferably dried peony root bark; the irradiation sterilization is cobalt 60 irradiation, plasma beam irradiation sterilization, preferably plasma beam irradiation sterilization; the irradiation intensity is 5000-8000 kGy, preferably 7000 kGy. The fermentation time is preferably 60 hours. In the centrifugation, the rotation speed is 6000-12000 r / min, preferably 10000 r / min.

[0056] Comparative Example 5

[0057] The present invention provides a method for preparing a jujube tree bark extract, comprising the following preparation steps:

[0058] The bark of the jujube tree is extracted with ethanol at a mass concentration of 30-75% for 2-4 hours and then filtered. The filtrate is collected for later use. The residue is then extracted with ethanol at a mass concentration of 20-65% for 2-3 hours and then filtered. The two filtrates are combined and concentrated, and then adsorbed with a macroporous resin, and then eluted with ethanol at a mass concentration of 30-70%. The eluate is concentrated under reduced pressure to obtain an extract.

[0059] Comparative Example 6

[0060] The present invention provides a method for preparing a peony root bark extract, comprising the following preparation steps:

[0061] The root bark of peony root is extracted with ethanol at a mass concentration of 30-75% for 2-4 hours and then filtered, the filtrate is collected for later use, and then the residue is extracted with ethanol at a mass concentration of 20-65% for 2-3 hours and then filtered, the two filtrates are combined and concentrated, and then adsorbed with a macroporous resin, and then eluted with ethanol at a mass concentration of 30-70%, and the eluate is concentrated under reduced pressure to obtain an extract.

[0062] Preparation of fermented plant-derived composite antibacterial agents of various proportions in Examples 4-6 and Comparative Examples 1-4:

[0063] According to the mass percentage composition in Table 1, the fermentation liquid, extract, synergistic enhancer and solvent are stirred evenly, mixed and dissolved, and the fermented plant extract composite antibacterial agents of various proportions can be obtained.

[0064] Table 1. Compound antibacterial agents of fermentation broth and plant extracts in various ratios.

[0065]

[0066]

[0067] Example 6 Application of the composition in skin care products

[0068] The application of the composition prepared in the above Example 4 and the formula of the sunscreen lotion product containing the composition are shown in Table 2.

[0069] Table 2 Sunscreen lotion product formula containing preservative composition

[0070]

[0071] Comparative Example 7 Skin Care Matrix Formula

[0072] The product formula of the sunscreen lotion without adding the composition prepared in Example 4 is shown in Table 3.

[0073] Table 3 Sunscreen lotion product formula without preservative composition

[0074]

[0075]

[0076] Test Example 1. Anticorrosion Challenge Comparative Experiment of Compositions

[0077] 1. Preparation of experimental bacterial suspension:

[0078] This antiseptic challenge experiment will adopt the mixed bacterial inoculation method, inoculating Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa on soybean casein agar (TSA) slant medium respectively, and culturing at (37±2)℃ for 48h. Wash the bacterial lawn with 5ml 0.85% sterile saline to make 1×10 7 ~10 8 CFU / ml bacterial suspension, the above three bacterial suspensions were mixed in equal volumes to prepare a mixed bacterial suspension, which was placed in a 4°C refrigerator for later use. Candida albicans and Aspergillus niger were inoculated on modified Martin agar slant medium, Candida albicans was cultured at (28±2)°C for 72h, Aspergillus niger was cultured at (28±2)°C for 5d, and the bacterial lawn was washed with 5ml of 0.85% sterile saline to prepare 1×10 6 ~10 7 CFU / ml bacterial suspension, mix the two fungal suspensions in equal volumes to prepare a mixed fungal suspension, which is stored in a 4°C refrigerator for later use.

[0079] 2. Anticorrosion Challenge Experiment

[0080] This experiment used a 28-day microbial challenge experiment with one-time addition of bacteria. On the first day of the experiment (i.e., day 0), microorganisms were inoculated into the test samples. The inoculated test samples were placed in an incubator at (25±2)°C, and then the test samples were taken out of the incubator on days 0, 7, 14, 21, and 28, respectively. The plate count method was used to determine the microbial concentration of each test sample. The specific steps are as follows.

[0081] Weigh 100g of each of the eight test samples under sterile conditions, weigh 2 portions of each sample, place them in a sterile container, add 1ml of mixed bacterial suspension and 1ml of mixed fungal suspension respectively, and shake well. Ensure that the bacterial content of each gram of sample added to the bacterial suspension is about 10 8 CFU / ml, added to the sample of fungal suspension, the bacterial count per gram of sample is about 10 6 CFU / ml. Weigh 10g of sample accurately, add it to a conical bottle containing glass balls and 90ml sterile saline, shake it thoroughly and mix it. This suspension is a 1:10 dilution, and then dilute it with saline in a 10-fold gradient. Take appropriate dilutions on days 0, 7, 14, 21, and 28, and use the plate pouring method to detect the number of colonies on days 0, 7, 14, 21, and 28 of inoculation. The results and analysis are shown in Table 2.

[0082] 3. Evaluation Criteria

[0083] This experiment refers to the CTFA method. If the number of surviving bacteria is below 100 CFU / g (CFU / ml) 7 days after adding bacteria, and all drops to 0 CFU / g (CFU / ml) within 28 days, it can be regarded as an excellent preservative effect and passes the preservative challenge test; if the number of surviving bacteria is below 1000 CFU / g (CFU / ml) on the 7th day, it is regarded as barely passed; if the number of surviving bacteria is above 1000 CFU / g (CFU / ml) on the 7th day, it is considered to have no preservative effect.

[0084] 4. Results Statistics

[0085] Table 4 Statistical table of microbial preservation challenge test results

[0086]

[0087]

[0088] According to the statistical results in Table 4, Examples 3, 4 and 5 passed the antiseptic challenge, indicating that the composition has an obvious antiseptic effect. Comparison of the results of Example 6 and Comparative Example 5 shows that the composition can significantly enhance the antiseptic ability of skin care products.

[0089] Test Example 2 Stability test of the composition

[0090] The compositions obtained according to Examples 3 to 5 and Comparative Examples 1 to 4 were numbered: Group A1, Group A2, Group A3, Group B1, Group B2, Group B3, Group B4. Samples from each group were placed in a cycle (stored at -18°C for 12 hours, taken out, stored at 4°C for 12 hours, placed at 45°C for 12 hours, placed at -18°C, and stored in a cycle in sequence), -18°C, 4°C, and 45°C, and the appearance changes of the skin barrier repair composition were observed at 1 week, 2 weeks, and 4 weeks, respectively, such as discoloration, precipitation, taste change, fungal growth, etc. If any appearance change occurs, the experimental result is marked as "×", and if the above appearance change does not occur, the experimental result is marked as "√". The results are shown in Table 5.

[0091] Table 5 Stability investigation results of the composition

[0092]

[0093] It can be seen from Table 5 that propylene glycol can increase the stability of the composition.

[0094] Test Example 3: Occluded patch test of the composition on sensitive skin

[0095] 1. Method introduction:

[0096] "Technical Specification for Safety of Cosmetics" (2015 Edition), Chapter 7, Section 2, Human Skin Closed Patch Test Method.

[0097] Human skin closed patch test method: Select a qualified patch tester and place 0.020-0.025mL of the test substance into the small chamber of the patch tester. Apply the patch tester with the test substance to the back of the subject for 24 hours. Remove the test substance patch tester after 24 hours, and observe the skin reaction at 0.5h, 24h, and 48h after removal. Record the observation results according to the skin reaction grading standards in the "Safety Technical Specifications for Cosmetics" (2015 Edition).

[0098] 2. Interpretation criteria

[0099] The skin reaction grading standards for skin occlusion patch test are shown in Table 6:

[0100] Table 6 Skin reaction grading standards for skin occlusive patch test

[0101] Degree of reaction Rating Skin reactions - 0 Negative reaction ± 1 Suspected reaction: only slight erythema + 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, and mounds ++ 3 Strong positive reaction (herpes reaction): erythema, infiltration, edema, papules, +++ 4 Very strong positive reaction (confluent herpes reaction): obvious erythema, severe infiltration

[0102] According to the interpretation of results in the "Cosmetic Hygiene Standards (2007 Edition)", the criteria for interpreting the results of the skin occluded patch test are: among 30 subjects, if more than 5 cases of Grade 1 adverse skin reactions occur, or more than 2 cases of Grade 2 adverse skin reactions occur, or if any case of Grade 3 or above adverse skin reaction occurs, the test substance is judged to have an adverse skin reaction to humans.

[0103] 3. Test information

[0104] Number of volunteers: 30

[0105] Volunteer skin type: Sensitive skin:

[0106] Volunteer age: 23 to 60 years old

[0107] Test area: back

[0108] 4. Test Results

[0109] According to the grading standards in Table 5, the skin reactions of the volunteers' test areas were scored 0.5 h, 24 h and 48 h after the spot tester was removed. The results are shown in Table 7.

[0110] Table 7 Test results at different time points (0.5h, 24h, 48h)

[0111]

[0112] According to the results in Table 7, the composition itself has no potential to cause adverse skin reactions, while the extracts obtained by ordinary extraction technology will have abnormal reactions at different levels, and the addition of the composition can also reduce the possibility of sunscreen lotion causing adverse reactions to the skin.

[0113] Antioxidant skin care effect of test example 4 composition

[0114] The promoting effect of the composition (Example 4) on the DPPH scavenging ability in HSF cells

[0115] HSF cells were cultured at 1×10 5 Cells were inoculated into 6-well plates at a seeding density of 100 cells / well. After incubation in an incubator (37°C, 5% CO2) for 24 hours, the cell plating rate reached 40% to 60%. A blank control group and sample groups with different final concentrations were set up and cultured in the incubator for another 24 hours. After the incubation, the cells were digested and collected, centrifuged at 2000 rpm for 10 minutes, and the supernatant was taken. The cells were grouped according to the figure below, and the OD value was detected at 517 nm to detect the changes in the DPPH scavenging ability of HSF cells. The results are shown in FIG. Figure 2 As shown, when the fermentation broth composition of Example 5 was used for intervention at a concentration of 0.2%, the DPPH scavenging ability thereof was increased by 7.2%, and when the fermentation broth composition was used for intervention at a concentration of 0.5%, the DPPH scavenging ability thereof was increased by 19.6%.

[0116] As shown in Tables 8 and 9, the single peony root fermentation product does not have a very significant DPPH scavenging ability.

[0117] Table 8 Comparison table of sample treatment in different groups

[0118]

[0119] Table 9 DPHP removal ability of test objects

[0120]

[0121] The experimental results show that the two extracts have no obvious antioxidant capacity. Comparative Example 1 shows that the antioxidant capacity of the fermented Ziziphus joazeiro bark is stronger than that of the ordinary extract. The combination of the two fermented products has the strongest antioxidant capacity. It can be seen that they have a synergistic effect.

[0122] It should be noted that the above examples are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention is described in detail with reference to the given examples, those skilled in the art may modify or replace the technical solution of the present invention as needed without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A probiotic fermentation composite plant preservative, characterized in that: The invention comprises the following components: probiotic jujube bark fermentation liquid, yeast peony root bark fermentation liquid, and synergistic enhancer; the mass ratio of the probiotic jujube bark fermentation liquid is 51-61%, the mass ratio of the yeast peony root bark fermentation liquid is 23-33%, and the mass ratio of the synergistic enhancer is 16%; the synergistic enhancer is propylene glycol; The preparation method of the probiotic jujube bark fermentation liquid comprises the following steps: (1) Wash the bark of the jujube tree with clean water, dry it at 60°C, crush it through a sieve with a pore size of 0.21 mm for later use, and sterilize it by irradiation; (2) Anaerobically culturing lactic acid bacteria using MRS medium at 37° C.; adding irradiated Ziziphus joaillerie bark powder in an amount of 5-12% by volume to the lactic acid bacteria culture solution and anaerobically fermenting for 48 hours to obtain a fermentation solution; (3) subjecting the fermentation liquid to solid-liquid centrifugation separation, and obtaining the supernatant as the probiotic Ziziphus jojoba bark fermentation liquid; The method for preparing the yeast peony root bark fermentation liquid comprises the following preparation steps: (1) Washing, crushing and drying the root bark of peony into powder, and sterilizing by irradiation; (2) The yeast was cultured in potato culture medium so that the live yeast content in the culture medium was ≥10 6 cfu / ml; (3) Adding irradiated peony root bark powder in a yeast culture medium at a mass ratio of 2-10%, fermenting for 48-72 hours to obtain a fermentation liquid; centrifuging the fermentation liquid to obtain a supernatant; centrifuging the supernatant again to obtain the final peony root bark fermentation liquid.

2. The probiotic fermentation composite plant preservative according to claim 1, characterized in that: In the method for preparing the probiotic jujube bark fermentation liquid, the lactic acid bacteria is one of Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus gasseri, and Lactobacillus helveticus; the jujube bark is dried or newly harvested jujube bark; the irradiation sterilization is cobalt 60 irradiation; and the centrifugal speed in step (3) is 6000-12000 r / min.

3. A probiotic fermentation composite plant preservative according to claim 2, characterized in that: The lactic acid bacteria is Lactobacillus rhamnosus, the jujube bark is dried jujube bark, the fermentation time is 60 hours, and the centrifugal speed in step (3) is 10000 r / min.

4. The probiotic fermentation composite plant preservative according to claim 1, characterized in that: In the method for preparing yeast peony root bark fermentation liquid, the peony root bark is dried or newly picked peony root bark; the irradiation sterilization is cobalt 60 irradiation; and the centrifugal speed in step (3) is 6000-12000r / min.

5. The probiotic fermentation composite plant preservative according to claim 4, characterized in that: The peony root bark is dried peony root bark, and the fermentation time is 60 hours; the centrifugal speed in step (3) is 10000 r / min.

6. Use of the probiotic fermentation composite plant preservative according to any one of claims 1 to 5 in the preparation of skin care products requiring preservation or anti-oxidation.

7. The use according to claim 6, characterized in that: The antiseptic or anti-oxidant skin care products include essences, lotions, creams and facial masks.

8. The use according to claim 7, characterized in that: The lotions include sunscreen lotions.

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