A staining reagent and a method for obtaining a microscopic structure of a large seaweed with small filaments having nodes

Abstract

The present invention discloses a staining reagent and a method for obtaining a microscopic structure of a large seaweed with noded filaments. The invention relates to the field of biological sample preparation methods. The staining reagent comprises the following components: p-chloro-meta-xylenol; an acidic reagent; a magenta reagent; a solvent; wherein the volume ratio of p-chloro-meta-xylenol, the acidic reagent and the solvent is 5:(45-80):(55-80); the acidic reagent comprises glacial acetic acid. The staining reagent of the present invention can stain out certain important features in the structure, such as nodes connecting pits, plasmodesmata, etc. These features are conducive to a clearer display of cortical development and tetrasporangium development.

Description

Technical Field

[0001] The invention relates to the field of biological sample preparation methods, in particular to a staining reagent and a method for obtaining a microscopic structure of a large seaweed with noded filamentous filaments. Background Art

[0002] Microscopic structural research is an essential means to reveal life phenomena and processes. It has been more than 300 years since Dutch scientist Hooke invented the microscope, and many advances have been made. However, research in this field is often affected by the quality of the preparation technology during sample processing.

[0003] The large seaweeds with small filaments and nodes are large lower plants in the ocean, mainly species in the order CERAMIALES, including species of genera such as Ceramiun, Centroceras, Polysiphonia, Neosiphonia, and Herposiphonia. Microscopic structural characteristics such as the cross-section of the algae, the origin of the tetrasporangium, the origin of the rhizoids, and the development of the cortex are important features for accurate species identification, classification, and research on life activities of these genera and species. However, the algae of these genera and species are too slender, with a width ranging from tens of micrometers to several millimeters, and it is very difficult to obtain clear microscopic structural characteristics. In the past, scholars in this field mainly used paraffin sections to obtain their microscopic structural characteristics. However, there are obvious shortcomings: 1) Paraffin sections need to go through multiple procedures such as fixation-washing-dehydration-transparent-wax dipping-embedding-slicing-slicing-slicing-baking, which is complicated, time-consuming and labor-intensive; 2) Paraffin sections require instruments such as dehydrators, embedding machines, and slicers, and various reagents are required in the above operation procedures, which has high investment costs; 3) Some algae are only more than 40 microns wide, which is very thin, and it is difficult to cut out ideal microstructures even with paraffin sections; 4) The microstructures obtained by paraffin sections are independent cross-sections, which are not complete and cannot intuitively reflect the continuous changes in the microstructural characteristics of a section of algae; 5) Paraffin sections cannot obtain cortical structural characteristics. Some scholars also use the method of staining and preparation, but they can only obtain cortical structures. Due to the defects of the formula, it is impossible to further obtain clearer and more complete key microstructures, and it is impossible to highlight important features in the structure through staining. For example, in the prior art, the nodes and intercellular filaments connected by the pits in the seaweed structure cannot be observed by staining methods.

[0004] How to quickly obtain the microscopic structure of large seaweed with segmented, filamentous structures is obviously a scientific problem. Summary of the invention

[0005] The first technical problem to be solved by the present invention is:

[0006] A staining reagent is provided.

[0007] The second technical problem to be solved by the present invention is:

[0008] Provided is a method for preparing the staining reagent.

[0009] In order to solve the first technical problem, the technical solution adopted by the present invention is:

[0010] A staining reagent comprising the following components:

[0011] p-Chloro-meta-xylenol;

[0012] Acidic reagents;

[0013] Magenta reagent;

[0014] Solvents;

[0015] Wherein, the volume ratio of p-chloro-meta-xylenol, acidic reagent and solvent is 5:(45-80):(55-80);

[0016] The acidic reagent includes glacial acetic acid.

[0017] According to the embodiments of the present invention, one of the technical solutions has at least one of the following advantages or beneficial effects:

[0018] 1. The staining reagent of the present invention, on the one hand, uses the magenta reagent in the staining reagent to stain the sample, highlighting certain important features in the structure, such as the nodes connected by the pits, etc. On the other hand, because the large seaweed with segmented fine filaments has various connections such as intercellular mucus and intercellular links between the cortical cells and the periaxial cells, between the periaxial cells, and between the periaxial cells and the axial cells, and the pit connections, there is density, so it is necessary to first use the corrosiveness of parachlorometaxylenol and acetic acid to dissociate the mucus and their connections between the cortical cells and the periaxial cells, between the periaxial cells, and between the periaxial cells and the axial cells, and then by controlling the volume ratio of parachlorometaxylenol, the acidic reagent and the solvent in the mixed reagent, the connection between the cells inside the cortex is retained.

[0019] 2. The dyeing reagent of the present invention can dye out some important features in the structure, such as nodes connected by pits, plasmodesmata, etc., through the combination of glacial acetic acid and magenta reagent, which are conducive to more clearly showing the development of cortex and tetrasporangium. In addition, the use of chlorometa-xylenol is more suitable for large seaweed with small and filamentous nodes, compared with phenol in existing dyeing reagents. After treatment with chlorometa-xylenol, various clear and complete key microscopic structures such as the rhizoid development mode, tetrasporangium development mode, periaxial cell number and its continuous change process, and cortical development process of large seaweed with small and filamentous nodes can be further obtained.

[0020] 3. The staining reagent of the present invention can promote cell wall separation and further make the intercellular filaments clear.

[0021] According to an embodiment of the present invention, the acidic reagent further includes lactic acid. By adding an acidic reagent including lactic acid to the acidic reagent, the corrosion effect of the acidic reagent can be promoted.

[0022] According to one embodiment of the present invention, the volume ratio of para-chloro-meta-xylenol, the acidic reagent and the solvent is 5:(45-50):(55-60).

[0023] According to one embodiment of the present invention, the amount of the magenta reagent added is excessive.

[0024] In order to solve the second technical problem, the technical solution adopted by the present invention is:

[0025] A method for obtaining a microstructure of a large seaweed with fine filaments having nodes, comprising the following steps:

[0026] S1 prepares the staining reagent;

[0027] S2 mixes the seaweed sample with the staining reagent to obtain a stained sample;

[0028] S3 separates the seaweed sample into sections, and each section is a cross section of a sample;

[0029] S4 cuts off the segments of the seaweed separated in step S3, spreads the broken segments flat, and observes the microstructure through a microscope.

[0030] According to the embodiments of the present invention, one of the technical solutions has at least one of the following advantages or beneficial effects:

[0031] 1. The method of the present invention has fast film-making speed, very low cost, is economical and applicable, and is conducive to industrial production.

[0032] 2. The method of the present invention has good dyeing effect, can obtain various complete and clear microstructures, and is not easy to fade. The sample obtained after dyeing has a bactericidal and antiseptic effect because the dyeing reagent contains parachlorometaxylenol and an acidic reagent, and can be stored for a long time.

[0033] 3. Through the method of the present invention, not only can various clear characteristic microscopic structures of large seaweed with fine filaments with nodes be obtained, but also the continuous changes of the microscopic structural characteristics of a section of algae can be intuitively reflected, which has important application value in the morphological, anatomic, developmental and evolutionary research of seaweed.

[0034] According to one embodiment of the present invention, in step S1, the staining reagent is prepared, including the following steps: mixing an acidic reagent in a solvent to obtain a mixed solution, boiling the mixed solution to boiling point, adding an excess of a magenta reagent, cooling and filtering, and adding para-chlorometaxylenol to the filtrate to obtain the staining reagent.

[0035] According to one embodiment of the present invention, in step S2, the sample and the staining reagent are mixed in a closed tank for 5-6 hours. The mixing time is based on whether the sample after corrosion can be easily opened by an acupuncture needle. In the present invention, by controlling the content of the component used for corrosion in the staining reagent and the time used for corrosion, the connection between the cells inside the cortex is retained to facilitate subsequent operations, such as opening the algae, the entire cortex, or obtaining a cross section.

[0036] According to one embodiment of the present invention, step S2 further includes the following steps: cleaning the seaweed sample with a brush.

[0037] According to one embodiment of the present invention, in step S3, the seaweed sample is separated into sections, and the separation tool used includes an acupuncture needle.

[0038] According to one embodiment of the present invention, step S3 further includes the following steps: firstly washing away the excess staining reagent in the seaweed sample with a solvent, and then adding a solvent to suspend the seaweed sample.

[0039] According to one embodiment of the present invention, the cross section obtained in step S3 can be directly photographed with selected features under 4X and 10X objective lenses without the need for a cover sheet.

[0040] According to one embodiment of the present invention, in step S4, the broken sections are spread out flat, which includes the following steps: spreading the broken sections flat on a glass slide and covering them with a coverslip.

[0041] According to an embodiment of the present invention, in step S4, the broken sections are flattened to obtain a cortical structure.

[0042] According to one embodiment of the present invention, after the sections are spread flat on a glass slide and covered with a cover slip, the steps of adding a moisturizing preservative and sealing the slide are also included.

[0043] Another aspect of the present invention also relates to the use of the staining reagent in morphology, anatomy, development or evolution. It includes the staining reagent as described in the embodiment of the first aspect above. Since the application adopts all the technical solutions of the staining reagent, it at least has all the beneficial effects brought by the technical solutions of the above embodiments.

[0044] Other features and advantages of the present invention will be set forth in the description which follows, and in part will be apparent from the description, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0046] Figure 1 This is a 400 μm microstructure image of the large seaweed sample with noded filaments before treatment in Example 1;

[0047] Figure 2 This is a 20 μm microstructure image of the noded, filamentous, large seaweed sample before treatment in Example 1;

[0048] Figure 3 This is a microscopic picture of the clustered rhizoids of the noded, filamentous, large seaweed sample at 400 μm before treatment in Example 1;

[0049] Figure 4 This is a microscopic structure of the tetrasporangium (T) ring of the noded filamentous macroalgae sample at 20 μm before treatment in Example 1;

[0050] Figure 5 This is a 100 μm microstructure image of the noded, filamentous, large seaweed sample before treatment in Example 2;

[0051] Figure 6 The microstructure of the large seaweed sample with small filaments and nodes obtained after the treatment and neutralization treatment in Example 1;

[0052] Figure 7 The microscopic structure of the cross section of the node of the large seaweed sample with small filaments and nodes obtained after being treated in Example 1;

[0053] Figure 8 The microscopic structure of the tetrasporangium (T) ring of the large seaweed sample with fine filaments and nodes obtained after being treated in Example 1;

[0054] Fig. 9 The microscopic structure of the cross section of the node of the tetrasporangium (T) obtained after the sample of the noded filamentous macroalgae was treated in Example 1;

[0055] Fig.10 This is a microscopic structure of the upper part of the algae body of the large seaweed sample with fine filaments and nodes obtained after being treated in Example 2;

[0056] Fig.11 This is a microscopic structure of the lower part of the algae body of the large seaweed sample with fine filaments and nodes after being treated in Example 2;

[0057] Fig.12 is a microscopic structure diagram of a large seaweed sample with small filaments and nodes after being treated in Example 1;

[0058] Fig.13 This is a microscopic structure of a large seaweed sample with small filaments and nodes after being treated with a comparative example;

[0059] Fig.14 This is a microscopic structure diagram of the perianth cells of the taro root Jiangli before being treated in Example 3;

[0060] Fig.15 This is a microscopic structure diagram of the capsule cells of the taro root Jiangli after being treated in Example 3;

[0061] Fig.16 This is a microscopic structure diagram of the capsule cell sample of the lycopodiella serrata before being treated by Example 4;

[0062] Fig.17 This is a microscopic structure diagram of the capsule cell sample of the heteropodial lily after being treated in Example 4. DETAILED DESCRIPTION

[0063] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of the present invention.

[0064] Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0065] Example 1

[0066] The large seaweed sample with small filaments and nodes selected in Example 1 is a species of the genus Ceramium gardneri Kylin.

[0067] A staining reagent comprising the following components:

[0068] 4-Chloro-meta-xylenol 5ml;

[0069] Glacial acetic acid 45ml;

[0070] Magenta reagent;

[0071] 55ml distilled water;

[0072] Among them, the amount of magenta reagent added is excessive.

[0073] A method for obtaining a microstructure of a large seaweed with fine filaments having nodes, comprising the following steps:

[0074] S1 prepares the dyeing reagent: 45 ml of glacial acetic acid, 55 ml of distilled water, boils the mixed solution to boiling point, adds excess magenta powder until it cannot be dissolved, cools it and filters it, then adds 5 ml of para-chloro-metaxylenol to the filtrate and mixes it to obtain a dyeing reagent;

[0075] S2 Select the target sample, clean the algae surface with a brush 2-3 times to remove impurities and algae on the algae surface, and cut the algae segment to be observed; put the selected sample segment into the staining tank, add the staining reagent, and seal the staining corrosion for about 5 hours;

[0076] S3 uses a needle to pick up the corroded sample and place it in a culture dish. Drip clean water to wash off the excess dye, then add clean water to lightly suspend the sample. Use an acupuncture needle to pick up the sample section by section under a dissecting microscope. Each section is a cross section of the sample. The cross section does not need a cover slide and can be directly photographed with features selected under a 4X and 10X objective lens.

[0077] S4 Use an acupuncture needle to cut the segment, carefully spread the segment flat on a glass slide, cover it with a coverslip to flatten the segment, and the flattened segment is the cortical structure.

[0078] S5 is sealed with moisturizing preservative.

[0079] Example 2

[0080] The difference between Example 2 and Example 1 is that different species are selected.

[0081] The large seaweed samples with small filaments and nodes selected in Example 2 are species of the genus Sphaerotheca.

[0082] A staining reagent comprising the following components:

[0083] 4-Chloro-meta-xylenol 5ml;

[0084] Glacial acetic acid 45ml;

[0085] Magenta reagent;

[0086] 55ml distilled water;

[0087] Among them, the amount of magenta reagent added is excessive.

[0088] A method for obtaining a microstructure of a large seaweed with fine filaments having nodes, comprising the following steps:

[0089] S1 Take the sample to be observed, clean the algae surface 2-3 times with a brush to remove impurities and algae on the algae surface, and cut the sample into algae segments with 3-4 limited branches;

[0090] S2 transfers the algae segment into a staining tank, adds staining reagent, and stains and corrodes for about 5 hours;

[0091] After S3 staining and dissociation, transfer the samples into a small beaker and wash them 3-4 times with distilled water to clean the stain on the surface of the algae segment. Then, place the algae segment flat on a clean glass slide, add water to cover the slide, and absorb the excess water.

[0092] S4 continuously presses the algae segment through the cover glass, and at the same time, places the sample under a microscope to observe whether it is pressed and spread, so that the algae segment cell monolayer is spread flat on the slide. Add 1-2 drops of sealing liquid and seal the slide.

[0093] S5 took photos under an imaging microscope to record microscopic features.

[0094] Example 3

[0095] The difference between Example 3 and Example 1 is that the samples are different. In Example 1, the sample selected is a large seaweed sample with small filaments and nodes, while in Example 3, the sample selected is a perianth cell sample of the taro root lily.

[0096] Example 4

[0097] The difference between Example 4 and Example 1 is that the samples are different. In Example 1, the sample selected is a large seaweed sample with small filaments and nodes, while in Example 4, the sample selected is a perianth cell sample of Glechoma heterophylla.

[0098] Comparative Example

[0099] The difference between the comparative example and Example 1 is that the dyeing reagent components are different. The dyeing reagent components of the comparative example are p-chloro-m-xylenol, lactic acid, aniline blue and distilled water. The dyeing reagent components of Example 1 are p-chloro-m-xylenol, glacial acetic acid, magenta reagent and distilled water.

[0100] After testing, it was found that the development of tetrasporangium could not be clearly observed in the large seaweed samples with segmented, filamentous and skeletal shapes after comparative staining.

[0101] Performance Test:

[0102] Microscopic structure photography was performed on the large seaweed sample with small filaments and nodes selected in Example 1: Ceramium gardneri Kylin, a species of the genus Ceramium.

[0103] in, Figure 1 This is a 400 μm microscopic structure of a large seaweed sample with small filaments and nodes before being treated in Example 1. Figure 1 In the figure, the cortical structure (C) and formation process of the large seaweed sample with segmented, filamentous filaments cannot be seen.

[0104] in, Figure 2 This is a 20 μm microstructure image of a large seaweed sample with fine filaments and nodes before being treated in Example 1.

[0105] in, Figure 3 This is a microscopic picture of the clustered rhizomes of a large seaweed sample with fine filaments and nodes at a depth of 400 μm before being treated in Example 1.

[0106] in, Figure 4 The microscopic structure of the tetrasporangium (T) ring of the large seaweed sample with fine filaments and joints at a depth of 20 μm before being treated in Example 1.

[0107] in, Figure 5 This is a 100 μm microscopic structure of a large seaweed sample with segmented, filamentous filaments before being treated in Example 2.

[0108] in, Figure 6 This is a microscopic structure diagram of a large seaweed sample with segmented, filamentous filaments obtained after being treated and neutralized in Example 1. Figure 6 A to I in the figure can clearly show the whole process of the gradual formation of mature cortical tissue from a periaxial cell (P) based on the intercellular filaments between cortical cells. Figure 6 A in the figure is a periaxial cell; Figure 6 B in the figure is the first cortical cell to divide into the first-order cortical cells at the upper left corner of the periaxial cell; Figure 6 C in the middle is the subsequent division of the first-order cortical cells in the upper right corner; Figure 6 D in the figure is the primary cortical cell which further divides into the secondary cortical cell; Figure 6 E in the figure shows that after the secondary cortical cells appear at the upper end of the periaxial cells, the primary cortical cells begin to divide at the lower left corner of the periaxial cells; Figure 6 F in the figure indicates that the third-order cortical cells begin to appear at the upper end of the periaxial cells. At the same time, the second-order cortical cells also begin to divide on the right side of the lower end of the periaxial cells. Figure 6 G in the figure indicates that the secondary cortical cells also begin to divide immediately at the lower left end of the periaxial cells; Figure 6 The H in the figure indicates that the 4th-order cortical cells begin to appear above the periaxial cells, and at the same time, a 1st-order cortical cell begins to split just below the periaxial cells. Figure 6 The I in the figure is a mature and complete cortical tissue, including the 4th-order cortical cells developed from the periaxial cells on the upper sides, the 3rd-order cortical cells developed on the lower sides, and the 2nd-order cortical cells developed directly below. Figure 6 The AI ​​in the figure is a complete cortical development process. It can be seen that the process of cortical development of the sample can be observed by the staining agent and method of the present invention.

[0109] in, Figure 7 This is a microscopic diagram of the cross-section of a large seaweed sample with fine filaments and nodes obtained after being treated in Example 1. Figure 7 It can be seen that the cross section of the node obtained after the treatment of Example 1 can clearly show the number of periaxial cells (P). Figure 2In the figure, the number of periaxial cells (P) cannot be displayed.

[0110] in, Figure 8 This is a microscopic image of the tetrasporangium (T) ring of a large seaweed sample with fine filaments and nodes obtained after being treated in Example 1. Figure 8 In the figure, it can be clearly shown that the rhizoids are produced by the periaxial cells (P) and the primary cortical cells (C). Figure 3 Clustered rhizoids can be seen in the figure, but it is unclear whether the rhizoids arise from periaxial cells (P) or cortical cells (C).

[0111] in, Fig. 9 The microscopic structure of the cross section of the tetrasporangium (T) obtained from the large seaweed sample with fine filaments and nodes after being treated in Example 1. Fig. 9 In the figure, based on the nodes connecting the tetrasporangium and the pits of the periaxial cells, it can be clearly seen that each periaxial cell (P) can produce two tetrasporangia (T). Figure 4 , it can be seen that the tetrasporangium (T) is arranged in a ring at the node, but it is impossible to know which cell the tetrasporangium (T) develops from.

[0112] in, Fig.10 This is a microscopic structure of the upper part of the large seaweed sample with segmented, filamentous lamina obtained after being treated in Example 2.

[0113] in, Fig.11 This is a microscopic structure of the lower part of the large seaweed sample with segmented, filamentous spherical structure obtained after being treated in Example 2.

[0114] from Fig.10 and Fig.11 In the figure, we can clearly see the number of periaxial cells and their changing patterns. Figure 5 In the figure, the number of periaxial cells cannot be seen.

[0115] Fig.12 : is a microscopic structure diagram of a large seaweed sample with small filaments and joints after being treated in Example 1. In this diagram, the development process of the tetrasporangium is clearly shown, wherein: Fig.12 A in the figure is a dichosporangium; Fig.12 B in the figure is a trisporangium; Fig.12 The C in the figure is a tetrasporangium.

[0116] Fig.13It is a microscopic structure diagram of a large seaweed sample with segmented filaments after being treated by a comparative example. After testing, it is impossible to clearly observe the development of tetrasporangium in the large seaweed sample with segmented filaments after staining in the comparative example. The difference between the comparative example and Example 1 is that the components of the staining reagent are different. Among them, the components of the staining reagent of the comparative example are p-chloro-meta-xylenol, lactic acid, aniline blue and distilled water. The components of the staining reagent of Example 1 are p-chloro-meta-xylenol, glacial acetic acid, magenta reagent and distilled water. It can be known that the use of the staining reagent of the embodiment of the present invention can clearly display the process of tetrasporangium development.

[0117] The capsule cell samples of the taro root Jiangli of Example 3 were tested, wherein: Fig.14 This is a microscopic structure diagram of the tegument cells of the taro root Jiangli before being treated by Example 3. Fig.15 This is a microscopic structure of the capsule cells of the taro root Jiangli after being treated in Example 3. Figure 14-15 It can be seen that in the processed microscopic structure diagram, the intercellular filaments can be clearly seen, thereby proving that the staining reagent of the present invention can promote cell wall separation and can further make the intercellular filaments become very clear.

[0118] The capsule cell samples of the scutellaria baicalensis of Example 4 were tested, wherein: Fig.16 This is a microscopic structure diagram of the perianth cell sample of the Ixodes heterophylla before being treated by Example 4. Fig.17 This is a microscopic structure of the capsule cell sample of the genus Ligusticum chuanxiong after being treated in Example 4. Figure 16-17 It can be seen that in the microscopic structure diagram after the treatment of Example 4, the intercellular filaments can be clearly seen, which proves that the staining reagent of the present invention can promote cell wall separation and can further make the intercellular filaments become very clear.

[0119] The above are only embodiments of the present invention, and are not intended to limit the patent scope of the present invention. Any equivalent transformations made using the contents of the present invention specification, or directly or indirectly applied in related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A method for obtaining the microstructure of a large seaweed with fine filaments using a staining agent, characterized in that: The staining reagent comprises the following components: p-Chloro-meta-xylenol; Acidic reagents; Magenta reagent; Solvents; The solvent is distilled water; Wherein, the volume ratio of the p-chloro-meta-xylenol, the acidic reagent and the solvent is 5:45-80:55-80; The acidic reagent includes glacial acetic acid; The preparation method of the staining reagent comprises the following steps: Mixing an acidic reagent in a solvent to obtain a mixed solution, boiling the mixed solution to boiling point, adding an excess amount of a carmine reagent, cooling and filtering, and adding parachloro-meta-xylenol to the filtrate to obtain a dyeing reagent; The method for obtaining the microstructure of large seaweed with noded filamentous filaments using the staining reagent comprises the following steps: S1: mixing the seaweed sample with the staining reagent to obtain a stained sample; S2 separates the seaweed sample into sections, and each section is a cross section of the sample; S3: cutting off the segments of the seaweed separated in step S2, spreading the broken segments flat, and observing the microstructure through a microscope.

2. The method according to claim 1, characterized in that: The acidic agent also includes lactic acid.

3. The method according to claim 1, characterized in that: The volume ratio of the p-chloro-meta-xylenol, the acidic reagent and the solvent is 5:45-50:55-60.

4. The method according to claim 1, characterized in that: In step S1, the sample and the staining reagent are mixed in a closed tank for 5-6 hours.

5. The method according to claim 1, characterized in that: Step S1 also includes the following steps: cleaning the seaweed sample with a brush.

6. The method according to claim 1, characterized in that: In step S2, the seaweed sample is separated into sections, and the separation tool used includes an acupuncture needle.

7. The method according to claim 1, characterized in that: Step S2 also includes the following steps: firstly, washing away the excess staining reagent in the seaweed sample with a solvent, and then adding a solvent to suspend the seaweed sample.

8. The method according to claim 1, characterized in that: In step S3, the broken section is spread out flat, which includes the following steps: spreading the broken section flat on a glass slide and covering it with a cover glass.

Images