A preparation method of probiotic emulsifier for feed production

Abstract

The invention discloses a method for preparing a probiotic emulsifier for feed production, and relates to the technical field of feed emulsifiers, including: preparing raw materials for preparing probiotic emulsifiers, preparing soybean lecithin slurry solutions, adding choline and mixing to prepare choline lecithin solutions, then adding polyoxyethylene ether aqueous solutions to obtain emulsifier base solutions, and then sequentially adding sucrose fatty acid esters, probiotics and stabilizers to obtain probiotic emulsifier solutions, and cooling, filtering, concentrating and drying to obtain finished probiotic emulsifiers. The present invention changes the head group structure of natural soybean lecithin by mixing natural soybean lecithin with choline solution, and mixes choline lecithin solution with polyoxyethylene ether aqueous solution to increase the emulsifier chain length, which is beneficial to improving the hydrophilicity and emulsification effect of the probiotic emulsifier, and is beneficial to improving the utilization rate of fat in feed, and by adding stabilizers, it is beneficial to increase the inactivation temperature of the emulsifier and enhance the applicability of the emulsifier.

Description

Technical Field

[0001] The invention relates to the technical field of feed emulsifiers, in particular to a method for preparing a probiotic emulsifier for feed production. Background Art

[0002] During the growth process of animals, nutrients such as oil or fat need to be supplemented, so oil or fat needs to be added to the feed. However, due to the limited secretion of bile salts in the intestines of animals, it is difficult to fully emulsify the fat in the feed, which greatly limits the digestion and absorption of oil. In order to improve the utilization rate of oil in feed, emulsifiers need to be added during feed production to assist the animal intestine in emulsifying oil or fat. Existing emulsifiers are mainly phospholipid emulsifiers, but they have poor hydrophilicity, poor emulsification effect, and poor stability, which is not convenient for feed pelleting and other processes.

[0003] The defects of existing feed emulsifiers are:

[0004] 1. In the patent document US06657123B2, the main consideration is to improve the dispersibility of the emulsifier, without considering the poor emulsification effect and poor emulsification stability of phospholipids or large proportions of phospholipids emulsifiers;

[0005] 2. The patent document US20070009644A1 mainly considers the problem of reducing the production cost of emulsifiers, but does not consider the problem that phospholipid emulsifiers have low inactivation temperature and poor stability;

[0006] 3. Patent document CN102640890B mainly considers the problem of improving the emulsification performance of livestock and poultry feed fat, but does not consider the problem that when the intestinal absorption capacity of livestock and poultry is poor, it is difficult to absorb emulsified fat and other substances;

[0007] 4. In the application document WO1995025434A1, the composition of the emulsifier includes one or more substances such as monocalcium phosphate, calcium chloride dihydrate, dicalcium hydrogen phosphate trihydrate and potassium chloride, but the problem that the introduction of more heavy metal ions will reduce the biological safety of the emulsifier is not considered. Summary of the invention

[0008] The object of the present invention is to provide a method for preparing a probiotic emulsifier for feed production, so as to solve the problems raised in the above background technology.

[0009] To achieve the above object, the present invention provides the following technical solution: a method for preparing a probiotic emulsifier for feed production, comprising the following steps:

[0010] Step 1: Prepare the raw materials for preparing the probiotic emulsifier, which include the following components: 80-120 parts of natural soybean lecithin, 50-80 parts of sucrose fatty acid ester, 40-60 parts of polyoxyethylene ether, 40-55 parts of choline, 60-80 parts of organic solvent, 20-40 parts of probiotics, 5-15 parts of stabilizer; 20-35 parts of water;

[0011] Step 2: Add natural soybean lecithin and organic solvent into a reaction kettle, heat to 50°C to 60°C, react for 1.5h to 2h, then cool to 20°C to 30°C, keep warm for 0.5h to 1h, and obtain a soybean lecithin slurry solution;

[0012] Step 3: Add choline to the soybean lecithin slurry solution, react at 50°C to 65°C for 1h to 2h to obtain a choline lecithin solution;

[0013] Step 4: Mixing polyoxyethylene ether with water and stirring evenly to obtain a polyoxyethylene ether aqueous solution;

[0014] Step 5: adding the polyoxyethylene ether aqueous solution to the choline phospholipid solution, heating to 50°C to 70°C, reacting for 2h to 3h to obtain an emulsifier base solution, and passing the emulsifier base solution into a homogenizer for high-pressure homogenization treatment;

[0015] Step 6: heat the sucrose fatty acid ester to 90°C to 100°C to melt, and then mix it with the homogenized emulsifier base solution, and react at a temperature of 65°C to 85°C for 1h to 1.5h, and then add probiotics and stabilizers, and react for 1h to 2h to obtain a probiotic emulsifier solution;

[0016] Step 7: Cooling the probiotic emulsifier solution to room temperature, filtering, concentrating and drying to obtain a finished probiotic emulsifier;

[0017] Step 8: Take the finished probiotic emulsifier to emulsify the added fat for animal feed, and record the duration of the fat emulsification process and the remaining fat content after the emulsification stops.

[0018] Preferably, in step 1, the mass ratio of natural soybean lecithin to organic solvent is (1-2.5): (1-1.5).

[0019] Preferably, the organic solvent is one or more of the green organic solvents butanediol, ethanol and propanol.

[0020] Preferably, the mass ratio of the choline to soybean lecithin slurry solution is (1-2):(4-10).

[0021] Preferably, the mass ratio of the polyoxyethylene ether to water is (2-3):(1-1.5), the stirring speed is 100-160 r / min, and the stirring time is 20-30 min.

[0022] Preferably, the emulsifier base solution is homogenized at 55-80 MPa for 1-5 times.

[0023] Preferably, the mass ratio of the sucrose fatty acid ester, the emulsifier base solution, the probiotics and the stabilizer is (10-15): (40-60): (1-3): (4-8).

[0024] Preferably, the probiotics are one or more of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium, and Lactobacillus gasseri, and the stabilizer includes one or more of citric acid, plant cellulose, and vitamin E.

[0025] Preferably, the probiotic emulsifier solution in step seven is filtered through a 28-mesh filter.

[0026] Preferably, in step S6, the following steps are also included:

[0027] S61: When the added stabilizer is plant cellulose, the plant cellulose is ground to 200-300 μm and then added to the emulsifier base solution.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] 1. The present invention dissolves natural soybean lecithin and mixes it with a choline solution to change the head group structure of natural soybean lecithin, mixes the choline lecithin solution with a polyoxyethylene ether aqueous solution to introduce hydrophilic functional groups, increase the emulsifier chain length, and improve the hydrophilicity of the emulsifier. By mixing with sucrose fatty acid esters with a high HBL value, the hydrophilicity of the probiotic emulsifier is improved, the emulsification effect is improved, and the utilization rate of fat in the feed is improved.

[0030] 2. The present invention helps to increase the inactivation temperature of the emulsifier and enhance the thermal stability of the emulsifier by adding a stabilizer, so that the emulsifier can be mixed with the feed production raw materials and then produced into feed through processes with higher temperatures such as granulation or puffing, thereby improving the applicability of the probiotic emulsifier.

[0031] 3. The present invention is beneficial to improving the biological activity of the emulsifier by adding probiotics, and provides a good growth environment for the beneficial bacteria in the animal intestine, improves the intestinal microenvironment of the animal, and improves the animal intestine's absorption capacity of nutrients in the feed.

[0032] 4. The present invention uses natural soybean lecithin as the emulsifier base material, which is easy to obtain, and uses one or more of the green organic solvents butanediol, ethanol, and propanol as the organic solvent to avoid the introduction of substances such as heavy metal ions, thereby improving the biosafety of the probiotic emulsifier and avoiding damage to the animal intestine. DETAILED DESCRIPTION

[0033] The following will be combined with the specific embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] The present invention provides a method for preparing a probiotic emulsifier for feed production, comprising the following steps:

[0035] Step 1: Prepare the raw materials for preparing the probiotic emulsifier, which include the following components: 80-120 parts of natural soybean lecithin, 50-80 parts of sucrose fatty acid ester, 40-60 parts of polyoxyethylene ether, 40-55 parts of choline, 60-80 parts of organic solvent, 20-40 parts of probiotics, 5-15 parts of stabilizer; 20-35 parts of water;

[0036] Step 2: Add natural soybean lecithin and organic solvent into a reaction kettle, heat to 50°C to 60°C, react for 1.5h to 2h, then cool to 20°C to 30°C, keep warm for 0.5h to 1h, and obtain a soybean lecithin slurry solution;

[0037] The mass ratio of natural soybean lecithin to organic solvent is (1-2.5): (1-1.5), and the organic solvent is one or more of green organic solvents such as butanediol, ethanol and propanol;

[0038] Step 3: Add choline to the soybean lecithin slurry solution, react at 50°C to 65°C for 1h to 2h to obtain a choline lecithin solution;

[0039] The mass ratio of choline to soybean lecithin slurry solution is (1-2): (4-10);

[0040] Step 4: Mixing polyoxyethylene ether with water and stirring evenly to obtain a polyoxyethylene ether aqueous solution;

[0041] The mass ratio of polyoxyethylene ether to water is (2-3): (1-1.5), the stirring speed is 100-160 r / min, and the stirring time is 20-30 min;

[0042] Step 5: adding the polyoxyethylene ether aqueous solution to the choline phospholipid solution, heating to 50°C to 70°C, reacting for 2h to 3h to obtain an emulsifier base solution, and passing the emulsifier base solution into a homogenizer for high-pressure homogenization treatment;

[0043] The homogenization treatment condition of the emulsifier base solution is 1 to 5 times at 55 to 80 MPa;

[0044] Step 6: heat the sucrose fatty acid ester to 90°C to 100°C to melt, and then mix it with the homogenized emulsifier base solution, and react at a temperature of 65°C to 85°C for 1h to 1.5h, and then add probiotics and stabilizers, and react for 1h to 2h to obtain a probiotic emulsifier solution;

[0045] The mass ratio of sucrose fatty acid ester, emulsifier base solution, probiotics and stabilizer is (10-15): (40-60): (1-3): (4-8).

[0046] The probiotics are one or more of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium, and Lactobacillus gasseri, and the stabilizer includes one or more of citric acid, plant cellulose, and vitamin E. When the added stabilizer is plant cellulose, the plant cellulose is ground to 200-300 μm and then added to the emulsifier base solution;

[0047] Step 7: Cooling the probiotic emulsifier solution to room temperature, filtering, concentrating and drying to obtain a finished probiotic emulsifier;

[0048] The probiotic emulsifier solution is filtered through a 28-mesh filter;

[0049] Step 8: Take the finished probiotic emulsifier to emulsify the added fat for animal feed, and record the duration of the fat emulsification process and the remaining fat content after the emulsification stops.

[0050] Embodiment 1:

[0051] Step 1: Prepare the raw materials for the probiotic emulsifier;

[0052] Step 2: Add 90 parts of natural soybean lecithin and 60 parts of organic solvent into a reactor, heat to 55°C, react for 1.5 hours, cool to 25°C, and keep warm for 0.6 hours to obtain a soybean lecithin slurry solution;

[0053] The organic solvent is a mixed solution of butanediol, ethanol and propanol, and the mass ratio is 1:1:1;

[0054] Step 3: Add 40 parts of choline to the soybean lecithin slurry solution, react at 55°C for 1.5 hours to obtain a choline lecithin solution, and change the head group structure of the lecithin;

[0055] Step 4: Mix 40 parts of polyoxyethylene ether with 25 parts of water, and stir and mix at 120 r / min for 20 minutes to obtain a polyoxyethylene ether aqueous solution;

[0056] Step 5: Add the polyoxyethylene ether aqueous solution to the choline phospholipid solution in step 3, heat to 60° C., and react for 2.5 hours to introduce polyoxyethylene chains into the choline phospholipid to obtain an emulsifier base solution, pass the emulsifier base solution into a homogenizer, and homogenize 3 times at 60 MPa to obtain a homogenized emulsifier base solution;

[0057] Step 6: Heat 55 parts of sucrose fatty acid ester to 95°C to melt, mix with the homogenized emulsifier base solution, heat to 70°C, mix and react at 250r / min for 1h, then add 25 parts of probiotics and 8 parts of stabilizer, react for 1.5h to obtain a probiotic emulsifier solution;

[0058] The probiotics are Lactobacillus acidophilus and Bifidobacterium, and the mass ratio is 1:1; the stabilizer is plant cellulose and vitamin E, and the mass ratio is 1:1.3; and the plant cellulose is ground into particles with a diameter of 260 μm by a grinding device;

[0059] Step 7: Cool the probiotic emulsifier solution to room temperature, filter it through a 28-mesh filter, and then concentrate and dry it to obtain a finished probiotic emulsifier;

[0060] Embodiment 2:

[0061] Step 1: Prepare the raw materials for the probiotic emulsifier;

[0062] Step 2: Add 100 parts of natural soybean lecithin and 75 parts of organic solvent into a reactor, heat to 55°C, react for 1.5 hours, then cool to 28°C, and keep warm for 0.8 hours to obtain a soybean lecithin slurry solution;

[0063] The organic solvent is a mixed solution of butanediol and propanol, and the mass ratio is 1:1;

[0064] Step 3: Add 40 parts of choline to the soybean lecithin slurry solution, react at 55°C for 1.5 hours to obtain a choline lecithin solution, and change the head group structure of the lecithin;

[0065] Step 4: Mix 45 parts of polyoxyethylene ether with 35 parts of water, and stir and mix at 120 r / min for 20 minutes to obtain a polyoxyethylene ether aqueous solution;

[0066] Step 5: Add the polyoxyethylene ether aqueous solution to the choline phospholipid solution in step 3, heat to 60° C., and react for 2.5 hours to introduce polyoxyethylene chains into the choline phospholipid to obtain an emulsifier base solution, pass the emulsifier base solution into a homogenizer, and homogenize 4 times at 60 MPa to obtain a homogenized emulsifier base solution;

[0067] Step 6: Heat 65 parts of sucrose fatty acid ester to 95°C to melt, mix with the homogenized emulsifier base solution, heat to 70°C, mix and react at 250r / min for 1h, then add 35 parts of probiotics and 8 parts of stabilizer, react for 1.5h to obtain a probiotic emulsifier solution;

[0068] The probiotics are Lactobacillus acidophilus and Bifidobacterium, and the mass ratio is 1:1; the stabilizer is plant cellulose and vitamin E, and the mass ratio is 1:1.3; and the plant cellulose is ground into particles with a diameter of 260 μm by a grinding device;

[0069] Step 7: Cool the probiotic emulsifier solution to room temperature, filter it through a 28-mesh filter, and then concentrate and dry it to obtain a finished probiotic emulsifier;

[0070] Embodiment three:

[0071] Step 1: Prepare the raw materials for the probiotic emulsifier;

[0072] Step 2: Add 100 parts of natural soybean lecithin and 75 parts of organic solvent into a reactor, heat to 60°C, react for 1.5 hours, cool to 30°C, and keep warm for 0.6 hours to obtain a soybean lecithin slurry solution;

[0073] The organic solvent is a mixed solution of butanediol and propanol, and the mass ratio is 1:1;

[0074] Step 3: Add 50 parts of choline to the soybean lecithin slurry solution, react at 60°C for 1.5 hours to obtain a choline lecithin solution, and change the head group structure of the lecithin;

[0075] Step 4: Mix 50 parts of polyoxyethylene ether with 35 parts of water, and stir and mix at 140 r / min for 25 minutes to obtain a polyoxyethylene ether aqueous solution;

[0076] Step 5: Add the polyoxyethylene ether aqueous solution to the choline phospholipid solution in step 3, heat to 60° C., and react for 2.5 hours to introduce polyoxyethylene chains into the choline phospholipid to obtain an emulsifier base solution, pass the emulsifier base solution into a homogenizer, and homogenize 4 times at 70 MPa to obtain a homogenized emulsifier base solution;

[0077] Step 6: Heat 70 parts of sucrose fatty acid ester to 95°C to melt, mix with the homogenized emulsifier base solution, heat to 70°C, mix and react at 250r / min for 1h, then add 30 parts of probiotics and 15 parts of stabilizer, react for 1.5h to obtain a probiotic emulsifier solution;

[0078] The probiotics are Lactobacillus acidophilus and Bifidobacterium, and the mass ratio is 1:1, and the stabilizers are citric acid and vitamin E, and the mass ratio is 1:1.5;

[0079] Step 7: Cool the probiotic emulsifier solution to room temperature, filter it through a 28-mesh filter, and then concentrate and dry it to obtain a finished probiotic emulsifier;

[0080] Embodiment 4:

[0081] Step 1: Prepare the raw materials for the probiotic emulsifier;

[0082] Step 2: Add 120 parts of natural soybean lecithin and 70 parts of organic solvent into a reactor, heat to 60°C, react for 1.5 hours, cool to 30°C, and keep warm for 0.6 hours to obtain a soybean lecithin slurry solution;

[0083] The organic solvent is a mixed solution of butanediol and propanol, and the mass ratio is 1:1;

[0084] Step 3: Add 50 parts of choline to the soybean lecithin slurry solution, react at 60°C for 1.5 hours to obtain a choline lecithin solution, and change the head group structure of the lecithin;

[0085] Step 4: Mix 45 parts of polyoxyethylene ether with 30 parts of water, and stir and mix at 140 r / min for 25 minutes to obtain a polyoxyethylene ether aqueous solution;

[0086] Step 5: Add the polyoxyethylene ether aqueous solution to the choline phospholipid solution in step 3, heat to 60° C., and react for 2.5 hours to introduce polyoxyethylene chains into the choline phospholipid to obtain an emulsifier base solution, pass the emulsifier base solution into a homogenizer, and homogenize 4 times at 70 MPa to obtain a homogenized emulsifier base solution;

[0087] Step 6: Heat 60 parts of sucrose fatty acid ester to 95°C to melt, mix with the homogenized emulsifier base solution, heat to 70°C, mix and react at 250r / min for 1h, then add 30 parts of probiotics and 10 parts of stabilizer, react for 1.5h to obtain a probiotic emulsifier solution;

[0088] The probiotics are Lactobacillus acidophilus and Bifidobacterium, and the mass ratio is 1:1, and the stabilizers are citric acid and vitamin E, and the mass ratio is 1:1.5;

[0089] Step 7: Cool the probiotic emulsifier solution to room temperature, filter it through a 28-mesh filter, and then concentrate and dry it to obtain a finished probiotic emulsifier;

[0090] Embodiment five:

[0091] Step 1: Prepare the raw materials for the probiotic emulsifier;

[0092] Step 2: Add 90 parts of natural soybean lecithin and 60 parts of organic solvent into a reactor, heat to 55°C, react for 1.5 hours, cool to 25°C, and keep warm for 0.6 hours to obtain a soybean lecithin slurry solution;

[0093] The organic solvent is a mixed solution of butanediol, ethanol and propanol, and the mass ratio is 1:1:1;

[0094] Step 3: Add 45 parts of choline to the soybean lecithin slurry solution, react at 55°C for 1.5 hours to obtain a choline lecithin solution, and change the head group structure of the lecithin;

[0095] Step 4: passing the choline phospholipid solution into a homogenizer and homogenizing it 3 times at 60 MPa to obtain a homogenized choline phospholipid solution;

[0096] Step 5: heat 55 parts of sucrose fatty acid ester to 95°C to melt, mix with the homogenized choline phospholipid solution, heat to 70°C, mix and react at 250r / min for 1h, then add 25 parts of probiotics, react for 1.5h, and obtain a probiotic emulsifier solution;

[0097] The probiotics are Lactobacillus acidophilus and Bifidobacterium, and the mass ratio is 1:1;

[0098] Step 6: Cool the probiotic emulsifier solution to room temperature, filter it through a 28-mesh filter, and then concentrate and dry it to obtain a finished probiotic emulsifier;

[0099] Embodiment six:

[0100] Step 1: Prepare the raw materials for the probiotic emulsifier;

[0101] Step 2: Add 90 parts of natural soybean lecithin and 60 parts of organic solvent into a reactor, heat to 55°C, react for 1.5 hours, cool to 25°C, and keep warm for 0.6 hours to obtain a soybean lecithin slurry solution;

[0102] The organic solvent is a mixed solution of butanediol, ethanol and propanol, and the mass ratio is 1:1:1;

[0103] Step 3: Add 45 parts of choline to the soybean lecithin slurry solution, react at 55°C for 1.5 hours to obtain a choline lecithin solution, and change the head group structure of the lecithin;

[0104] Step 4: Mix 45 parts of polyoxyethylene ether with 30 parts of water, and stir and mix at 120 r / min for 20 minutes to obtain a polyoxyethylene ether aqueous solution;

[0105] Step 5: Add the polyoxyethylene ether aqueous solution to the choline phospholipid solution in step 3, heat to 60° C., and react for 2.5 hours to introduce polyoxyethylene chains into the choline phospholipid to obtain an emulsifier base solution, pass the emulsifier base solution into a homogenizer, and homogenize 3 times at 60 MPa to obtain a homogenized emulsifier base solution;

[0106] Step 6: Add 30 parts of probiotics and 10 parts of stabilizer to the homogenized emulsifier base solution, react for 1.5 hours, and obtain a probiotic emulsifier solution;

[0107] The probiotics are Lactobacillus acidophilus, Bifidobacterium and Lactobacillus plantarum in a mass ratio of 1:1:1, the stabilizers are plant cellulose and vitamin E in a mass ratio of 1:2, and the plant cellulose is ground into particles with a diameter of 280 μm by a grinding device;

[0108] Step 7: Cool the probiotic emulsifier solution to room temperature, filter it through a 28-mesh filter, and then concentrate and dry it to obtain a finished probiotic emulsifier;

[0109] Embodiment 1:

[0110] Step 1: Prepare the raw materials for the probiotic emulsifier;

[0111] Step 2: Add 120 parts of natural soybean lecithin and 70 parts of organic solvent into a reactor, heat to 55°C, react for 1.5 hours, cool to 25°C, and keep warm for 0.6 hours to obtain a soybean lecithin slurry solution;

[0112] The organic solvent is a mixed solution of butanediol, ethanol and propanol, and the mass ratio is 1:1:1;

[0113] Step 3: Mix 45 parts of polyoxyethylene ether with 30 parts of water, and stir and mix at 120 r / min for 20 minutes to obtain a polyoxyethylene ether aqueous solution;

[0114] Step 4: Add the polyoxyethylene ether aqueous solution to the choline phospholipid solution in step 3, heat to 60° C., and react for 2.5 hours to introduce polyoxyethylene chains into the choline phospholipid to obtain an emulsifier base solution, pass the emulsifier base solution into a homogenizer, and homogenize 3 times at 60 MPa to obtain a homogenized emulsifier base solution;

[0115] Step 5: Heat 60 parts of sucrose fatty acid ester to 95°C to melt, mix with the homogenized emulsifier base solution, heat to 70°C, mix and react at 250r / min for 1h, then add 30 parts of probiotics and 10 parts of stabilizer, react for 1.5h to obtain a probiotic emulsifier solution;

[0116] The probiotics are Lactobacillus acidophilus and Bifidobacterium, and the mass ratio is 1:1; the stabilizer is plant cellulose and vitamin E, and the mass ratio is 1:1.3; and the plant cellulose is ground into particles with a diameter of 260 μm by a grinding device;

[0117] Step 6: Cool the probiotic emulsifier solution to room temperature, filter it through a 28-mesh filter, and then concentrate and dry it to obtain a finished probiotic emulsifier;

[0118] Take a unit mass portion of the added fat for feed production, and take the same mass portion of the finished probiotic emulsifier in Example 1, Example 2, Example 3, Example 4, Example 5, Example 6 and Example 7, respectively add the unit mass portion of the fat to multiple groups of beakers filled with equal amounts of aqueous solution, add a unit mass of fat to each group of beakers, and then add the finished probiotics prepared in Example 1 to Example 7 to the beakers, respectively, place the beakers at room temperature and heat the beakers in water at 30°C, 50°C, 60°C, 70°C, 80°C and 90°C, respectively, record the time when the unit fat stops emulsification, and the remaining content of the unemulsified fat when the fat stops emulsification;

[0119] The finished probiotic emulsifier components are as follows:

[0120]

[0121] The emulsification time of the added fat for feed production under different temperature conditions and the remaining content of the unemulsified fat when the fat stops emulsification are as follows:

[0122]

[0123]

[0124] In summary, according to Example 1, Example 2, Example 5, and Example 7, it can be known that by dissolving natural soybean lecithin and mixing it with a choline solution, the head group structure of natural soybean lecithin is changed, and by mixing a choline lecithin solution with a polyoxyethylene ether aqueous solution, a hydrophilic functional group is introduced to increase the emulsifier Liancheng, and by mixing with a sucrose fatty acid ester with a high HBL value, the hydrophilicity of the probiotic emulsifier is improved, the emulsification effect is improved, and it is beneficial to improve the utilization rate of fat in the feed;

[0125] According to Example 2, Example 4 and Example 5, the addition of a stabilizer is beneficial to increasing the inactivation temperature of the emulsifier, enhancing the thermal stability of the emulsifier, and enabling the emulsifier to be added to feeds of different forms through processes with higher temperatures such as granulation or puffing, thereby improving the practicality of the probiotic emulsifier.

[0126] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

Claims

1. A method for preparing a probiotic emulsifier for feed production, characterized in that: The following steps are involved: Step 1: Prepare raw materials for preparing probiotic emulsifier, which raw materials include the following components: 80-120 parts of natural soybean lecithin, 50-80 parts of sucrose fatty acid ester, 40-60 parts of polyoxyethylene ether, 40-55 parts of choline, 60-80 parts of organic solvent, 20-40 parts of probiotics, 5-15 parts of stabilizer; 20-35 parts of water; Step 2: Add natural soybean lecithin and organic solvent into a reaction kettle, heat to 50°C to 60°C, react for 1.5h to 2h, then cool to 20°C to 30°C, keep warm for 0.5h to 1h, and obtain a soybean lecithin slurry solution; Step 3: Add choline to the soybean lecithin slurry solution, react at 50°C to 65°C for 1h to 2h to obtain a choline lecithin solution; Step 4: Mixing polyoxyethylene ether with water and stirring evenly to obtain a polyoxyethylene ether aqueous solution; Step 5: adding the polyoxyethylene ether aqueous solution to the choline phospholipid solution, heating to 50°C to 70°C, reacting for 2h to 3h to obtain an emulsifier base solution, and passing the emulsifier base solution into a homogenizer for high-pressure homogenization treatment; Step 6: heat the sucrose fatty acid ester to 90°C to 100°C to melt, and then mix it with the homogenized emulsifier base solution, and react at a temperature of 65°C to 85°C for 1h to 1.5h, and then add probiotics and stabilizers, and react for 1h to 2h to obtain a probiotic emulsifier solution; Step 7: Cooling the probiotic emulsifier solution to room temperature, filtering, concentrating and drying to obtain a finished probiotic emulsifier; Step 8: Take the finished probiotic emulsifier to emulsify the added fat for animal feed, and record the duration of the fat emulsification process and the remaining fat content after the emulsification stops; The homogenization treatment condition of the emulsifier base solution is 1 to 5 times at 55 to 80 MPa; The stabilizer includes one or more of citric acid, plant cellulose and vitamin E.

2. The method for preparing a probiotic emulsifier for feed production according to claim 1, characterized in that: In the step 1, the mass ratio of natural soybean lecithin to organic solvent is (1-2.5): (1-1.5).

3. The method for preparing a probiotic emulsifier for feed production according to claim 2, characterized in that: The organic solvent is one or more of the green organic solvents butanediol, ethanol and propanol.

4. The method for preparing a probiotic emulsifier for feed production according to claim 1, characterized in that: The mass ratio of the choline to soybean lecithin slurry solution is (1-2): (4-10).

5. The method for preparing a probiotic emulsifier for feed production according to claim 1, characterized in that: The mass ratio of the polyoxyethylene ether to water is (2-3): (1-1.5), the stirring speed is 100-160 r / min, and the stirring time is 20-30 min.

6. The method for preparing a probiotic emulsifier for feed production according to claim 1, characterized in that: The probiotics are one or more of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium, and Lactobacillus gasseri.

7. The method for preparing a probiotic emulsifier for feed production according to claim 1, characterized in that: The probiotic emulsifier solution in step seven is filtered through a 28-mesh filter.

8. The method for preparing a probiotic emulsifier for feed production according to claim 1, characterized in that: In the step six, when the stabilizer added is plant cellulose, the plant cellulose is ground to 200-300 μm and then added to the emulsifier base solution.

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