Application method of nanoparticle preparation technology in cosmetics

Abstract

The present invention relates to an application method of nanoparticle preparation technology in cosmetics, belonging to the technical field of emulsions. The application method comprises the following steps: S1, caprylic acid / capric acid triglyceride, lupin protein, caffeic acid, octenyl succinic anhydride modified starch, astaxanthin and deionized water are mixed to obtain a mixed solution; S2, the mixed solution is emulsified, and after standing and defoaming, an emulsion is obtained; the starch is modified by octenyl succinic anhydride, and the starch is mixed with lupin protein and caffeic acid to prepare an emulsion, and the presence of lupin protein, caffeic acid and octenyl succinic anhydride modified starch in the emulsion can synergistically improve the storage stability of astaxanthin.

Description

Technical Field

[0001] The invention belongs to the technical field of emulsions and relates to an application method of nanoparticle preparation technology in cosmetics. Background Art

[0002] Natural astaxanthin has significant antioxidant properties. However, its highly unsaturated structure makes it at risk of chemical degradation under conditions such as high temperature and light, resulting in fading and reduced biological activity. This has, to a certain extent, limited the application of natural astaxanthin in food, medicine, cosmetics and other fields. In order to improve the utilization of astaxanthin in these fields, researchers have explored a variety of stabilization technologies, including emulsion encapsulation technology, microencapsulation technology, liposomes and nano-scale encapsulation technology.

[0003] Emulsions have a wide range of applications in the food, cosmetics and other industries, and therefore have important practical value. Many products exist in the form of emulsions. The traditional method of stabilizing emulsions is to add surfactants (such as polysorbates, phospholipids). However, for emulsions of this system, especially in cosmetic applications, surfactants often show adverse effects, such as irritation. In addition, such emulsions are prone to phase separation under the influence of heat and gravity, which is a problem faced by traditional emulsions. Summary of the invention

[0004] The purpose of the present invention is to provide an application method of nanoparticle preparation technology in cosmetics, wherein starch is modified by octenyl succinic anhydride, and an emulsion is prepared by modifying the starch with lupin protein and caffeic acid. The presence of lupin protein, caffeic acid and starch modified by octenyl succinic anhydride in the emulsion can synergistically improve the storage stability of astaxanthin.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A nanoparticle preparation technology comprises the following steps:

[0007] 1) Stir the starch base and ammonia water uniformly, perform ultrasonic treatment, freeze-dry, add sulfuric acid solution, stir and mix, let stand, filter, wash the solid, add deionized water, heat and mix, and obtain a suspension;

[0008] 2) After mixing the suspension and octenyl succinic anhydride, adjusting the pH value and stirring the reaction, adjusting the pH value to neutral, centrifuging, washing, and obtaining modified starch.

[0009] As a preferred technical solution of the present invention, in step 1), the stirring time is 2-3h; the stirring and mixing conditions are at a temperature of 38-40°C, a speed of 150-200r / min, and stirring for 4.5-5.0d; the washing conditions are washing with deionized water until neutral; the heating and mixing conditions are at a temperature of 30-60°C and stirring for 20-30min.

[0010] As a preferred technical solution of the present invention, in step 1), the conditions of the ultrasonic treatment are 40KHz frequency, 100W power, and ultrasonic treatment for 3min.

[0011] As a preferred technical solution of the present invention, in step 1), the usage ratio of the starch base and ammonia water is 1.0-1.2g:8mL; before the hydrolysis of the starch base, a small amount of ammonia water is added to enhance the thermal stability of the starch base and prevent the hydrolysis reaction from being too violent; further, ammonia water will affect the surface charge of starch particles, reduce electrostatic repulsion, and make the starch-based particles more stable, thereby inhibiting the damage caused by strong shear force and stress caused by ultrasound, and can effectively protect the structural integrity of starch particles.

[0012] As a preferred technical solution of the present invention, in step 1), the mass concentration of the ammonia water is 1.2-1.5%; the concentration of the sulfuric acid solution is 3.16 mol / L; the usage ratio of the starch base, sulfuric acid solution and deionized water is 14.7 g:100 mL:16-18 g.

[0013] As a preferred technical solution of the present invention, in step 2), the condition for adjusting the pH value is to adjust the pH value to 8.5-8.8 using a 3% mass concentration sodium hydroxide aqueous solution; the stirring reaction is adjusted to stir for 5.0-5.5h at a speed of 450-600r / min; the condition for adjusting the pH value to neutrality is to adjust it using a 0.1mol / L hydrochloric acid aqueous solution; and the washing condition is to wash with deionized water until the filtrate is neutral.

[0014] As a preferred technical solution of the present invention, in step 2), the mass ratio of the suspension to octenyl succinic anhydride is 30:0.47-0.52.

[0015] A method for applying nanoparticle preparation technology in cosmetics, the application method comprising the following steps:

[0016] S1, mixing caprylic / capric triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water to obtain a mixed solution;

[0017] S2. Emulsifying the mixed solution, standing it to defoam, and obtaining an emulsion.

[0018] As a preferred technical solution of the present invention, in step S1, the weight ratio of caprylic / capric triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water is 70: 0.50-0.55: 0.20-0.25: 1.2-1.5: 10: 35;

[0019] As a preferred technical solution of the present invention, in step S2, the emulsification condition is emulsification for 2 minutes at a rotation speed of 10000 r / min.

[0020] As a preferred technical solution of the present invention, octenyl succinic anhydride is subjected to an acylation reaction with the hydroxyl functional groups on starch, the chemical structure of the surface of the acylated starch nanocrystals is changed, and part of the hydroxyl groups are replaced by acyl groups, thereby obtaining octenyl succinic anhydride-modified starch. The grafting of acetyl groups increases the negative charge on the surface of the starch particles, and its amphiphilicity is improved, with better emulsification ability. Furthermore, lupin protein and octenyl succinic anhydride-modified starch can synergistically encapsulate astaxanthin and form a composite protection to prevent it from being destroyed by a high temperature environment.

[0021] As a preferred technical solution of the present invention, the emulsion contains polyphenols (caffeic acid) which is beneficial to protect the internal astaxanthin against adverse environments. Specifically, caffeic acid, as an antioxidant, inhibits the oxidation reaction, thereby reducing the loss of astaxanthin in a high temperature environment.

[0022] As a preferred technical solution of the present invention, lupin protein and starch modified with octenylsuccinic anhydride both have good emulsifying and thickening properties, which are helpful for the dispersion and stabilization of astaxanthin in the emulsion, thereby reducing the possibility of thermal decomposition; further, under the action of caffeic acid, the interaction between astaxanthin and natural lupin protein and starch components modified with octenylsuccinic anhydride is promoted to form a self-assembled form of water-soluble astaxanthin, wherein these natural components are wrapped around the outside of astaxanthin to make it stable in water solubility, and the astaxanthin in the obtained astaxanthin aqueous solution does not aggregate, etc., which significantly increases the stability of astaxanthin in the emulsion system.

[0023] As a preferred technical solution of the present invention, the starch base is one or more of the starch of glutinous corn, corn, wheat, potato, early indica rice, waxy rice and cassava.

[0024] As a preferred technical solution of the present invention, starch nanocrystals are polyhydroxy polymers, and each glucose structural unit has three hydroxyl groups on its surface, forming certain intermolecular and intramolecular hydrogen bonds.

[0025] As a preferred technical solution of the present invention, the emulsion prepared by the solution of the present invention can be used with the following components to prepare skin care products, including but not limited to water, glycerin, isopropyl myristate, propylene glycol, cetearyl alcohol, jojoba seed oil, evening primrose oil, hydrogenated polyisobutene, white beeswax, cyclopentasiloxane, caprylic / capric triglyceride, 1,3-propylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, polydimethylsiloxane, glyceryl stearate, cetearyl glucoside, cyclohexasiloxane, PEG-100 stearate, carbomer, squalane, arginine, bisabolol; and other trace ingredients: vanillyl butyl ether, Guangxi Curcuma zedoaria extract, sodium hyaluronate, disodium EDTA, sodium heparin, (daily) flavor, caprylhydroxamic acid , butylene glycol, tetrahydropiperine, caprylyl glycol, tetrahydromethylpyrimidine carboxylic acid, hydroxyethylcellulose, acrylates / C10-30 alkyl acrylate crosspolymer, palm leaf extract, lutein, shrimp spine orchid extract, Italian immortelle extract, mugwort leaf extract, oligopeptide-1, deoxyribonucleic acid, trehalose, phenoxyethanol, Ruscus aconitifolia root extract, ginger root extract, angelica root extract, safflower extract, white flower hedyotis diffusa extract, sea buckthorn fruit extract, citron peel extract, goldenrod extract, xanthan gum, calcium gluconate, hexylene glycol, polysorbate-20, sodium benzoate, ethylhexylglycerin, potassium sorbate, acetyl hexapeptide-8, hexapeptide-9, palmitoyl pentapeptide-4, gluconolactone, arginine / lysine polypeptide

[0026] Further, the method of using the skin care product is: wipe the skin with a hot towel before use or use this product after bathing, apply it on the body, shoulders, neck and back and massage for absorption.

[0027] Beneficial effects of the present invention:

[0028] The invention modifies starch by octenyl succinic anhydride, and prepares an emulsion by modifying the starch with lupin protein and caffeic acid. The presence of lupin protein, caffeic acid and starch modified by octenyl succinic anhydride in the emulsion can synergistically improve the storage stability of astaxanthin. DETAILED DESCRIPTION

[0029] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0030] Example 1

[0031] A nanoparticle preparation technology comprises the following steps:

[0032] 1) Stir corn starch and ammonia water evenly, perform ultrasonic treatment, freeze-dry, add sulfuric acid solution, stir and mix, let stand, filter, wash the solid, add deionized water, heat and mix, and obtain a suspension; wherein, the stirring time is 120 minutes; the ultrasonic treatment conditions are 40KHz frequency, 100W power, and ultrasonic treatment for 3 minutes; the stirring and mixing conditions are 38°C temperature, 150r / min speed, and stirring for 4.5 days; the washing conditions are washing with deionized water until neutral; the heating and mixing conditions are stirring at 30°C temperature for 20 minutes; the usage ratio of corn starch and ammonia water is 1.0g:8mL; the mass concentration of ammonia water is 1.2%; the concentration of sulfuric acid solution is 3.16mol / L; the usage ratio of corn starch, sulfuric acid solution and deionized water is 14.7g:100mL:16g;

[0033] 2) After mixing the suspension and octenyl succinic anhydride, adjusting the pH value and stirring the reaction, adjusting the pH value to neutral, centrifuging, washing, and obtaining modified starch; wherein the mass ratio of the suspension to octenyl succinic anhydride is 30:0.47; the condition for adjusting the pH value is to use a 3% mass concentration of sodium hydroxide aqueous solution to adjust the pH value to 8.5; the stirring reaction is adjusted at a speed of 450r / min and stirred for 5.0h; the condition for adjusting the pH value to neutral is to use a 0.1mol / L hydrochloric acid aqueous solution for adjustment; the washing condition is to use deionized water to wash until the filtrate is neutral;

[0034] A method for applying nanoparticle preparation technology in cosmetics, the application method comprising the following steps:

[0035] S1, mixing caprylic acid / capric acid triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water to obtain a mixed solution; wherein the weight ratio of caprylic acid / capric acid triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water is 70:0.50:0.20:1.2:10:35;

[0036] S2. Emulsifying the mixed solution, standing it for defoaming, and obtaining an emulsion; wherein the emulsification condition is emulsifying at a speed of 10000 r / min for 2 min.

[0037] Example 2

[0038] A nanoparticle preparation technology comprises the following steps:

[0039] 1) Stir corn starch and ammonia water evenly, perform ultrasonic treatment, freeze-dry, add sulfuric acid solution, stir and mix, let stand, filter, wash the solid, add deionized water, heat and mix, and obtain a suspension; wherein, the stirring time is 132 minutes; the ultrasonic treatment conditions are 40KHz frequency, 100W power, and ultrasonic treatment for 3 minutes; the stirring and mixing conditions are 38.4°C temperature, 160r / min speed, and stirring for 4.6 days; the washing conditions are washing with deionized water until neutral; the heating and mixing conditions are stirring at 36°C temperature for 22 minutes; the usage ratio of corn starch and ammonia water is 1.04g:8mL; the mass concentration of ammonia water is 1.26%; the concentration of sulfuric acid solution is 3.16mol / L; the usage ratio of corn starch, sulfuric acid solution and deionized water is 14.7g:100mL:16.4g;

[0040] 2) After mixing the suspension and octenyl succinic anhydride, adjusting the pH value and stirring the reaction, adjusting the pH value to neutral, centrifuging, washing, and obtaining modified starch; wherein the mass ratio of the suspension to octenyl succinic anhydride is 30:0.48; the condition for adjusting the pH value is to use a 3% mass concentration of sodium hydroxide aqueous solution to adjust the pH value to 8.56; the stirring reaction is adjusted at a speed of 480r / min and stirred for 5.1h; the condition for adjusting the pH value to neutral is to use a 0.1mol / L hydrochloric acid aqueous solution for adjustment; the washing condition is to use deionized water to wash until the filtrate is neutral;

[0041] A method for applying nanoparticle preparation technology in cosmetics, the application method comprising the following steps:

[0042] S1, mixing caprylic acid / capric acid triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water to obtain a mixed solution; wherein the weight ratio of caprylic acid / capric acid triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water is 70:0.51:0.21:1.26:10:35;

[0043] S2. Emulsifying the mixed solution, standing it for defoaming, and obtaining an emulsion; wherein the emulsification condition is emulsifying at a speed of 10000 r / min for 2 min.

[0044] Example 3

[0045] A nanoparticle preparation technology comprises the following steps:

[0046] 1) Stir corn starch and ammonia water evenly, perform ultrasonic treatment, freeze-dry, add sulfuric acid solution, stir and mix, let stand, filter, wash the solid, add deionized water, heat and mix, and obtain a suspension; wherein, the stirring time is 144 minutes; the ultrasonic treatment conditions are 40KHz frequency, 100W power, and ultrasonic treatment for 3 minutes; the stirring and mixing conditions are 38.8°C temperature, 170r / min speed, and stirring for 4.7 days; the washing conditions are washing with deionized water until neutral; the heating and mixing conditions are 42°C temperature and stirring for 24 minutes; the usage ratio of corn starch and ammonia water is 1.08g:8mL; the mass concentration of ammonia water is 1.32%; the concentration of sulfuric acid solution is 3.16mol / L; the usage ratio of corn starch, sulfuric acid solution and deionized water is 14.7g:100mL:16.8g;

[0047] 2) After mixing the suspension and octenyl succinic anhydride, adjusting the pH value and stirring the reaction, adjusting the pH value to neutral, centrifuging, washing, and obtaining modified starch; wherein the mass ratio of the suspension to octenyl succinic anhydride is 30:0.49; the condition for adjusting the pH value is to use a 3% mass concentration of sodium hydroxide aqueous solution to adjust the pH value to 8.62; the stirring reaction is adjusted at a speed of 510r / min and stirred for 5.2h; the condition for adjusting the pH value to neutral is to use a 0.1mol / L hydrochloric acid aqueous solution for adjustment; the washing condition is to use deionized water to wash until the filtrate is neutral;

[0048] A method for applying nanoparticle preparation technology in cosmetics, the application method comprising the following steps:

[0049] S1, mixing caprylic acid / capric acid triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water to obtain a mixed solution; wherein the weight ratio of caprylic acid / capric acid triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water is 70:0.52:0.22:1.32:10:35;

[0050] S2. Emulsifying the mixed solution, standing it for defoaming, and obtaining an emulsion; wherein the emulsification condition is emulsifying at a speed of 10000 r / min for 2 min.

[0051] Example 4

[0052] A nanoparticle preparation technology comprises the following steps:

[0053] 1) Stir corn starch and ammonia water evenly, perform ultrasonic treatment, freeze-dry, add sulfuric acid solution, stir and mix, let stand, filter, wash the solid, add deionized water, heat and mix, and obtain a suspension; wherein, the stirring time is 156 minutes; the ultrasonic treatment conditions are 40KHz frequency, 100W power, and ultrasonic treatment for 3 minutes; the stirring and mixing conditions are 39.2°C temperature, 180r / min speed, and stirring for 4.8 days; the washing conditions are washing with deionized water until neutral; the heating and mixing conditions are 48°C temperature and stirring for 26 minutes; the dosage ratio of corn starch to ammonia water is 1.12g:8mL; the mass concentration of ammonia water is 1.38%; the concentration of sulfuric acid solution is 3.16mol / L; the dosage ratio of corn starch, sulfuric acid solution and deionized water is 14.7g:100mL:17.2g;

[0054] 2) After mixing the suspension and octenyl succinic anhydride, adjusting the pH value and stirring the reaction, adjusting the pH value to neutral, centrifuging, washing, and obtaining modified starch; wherein the mass ratio of the suspension to octenyl succinic anhydride is 30:0.5; the condition for adjusting the pH value is to use a 3% mass concentration of sodium hydroxide aqueous solution to adjust the pH value to 8.68; the stirring reaction is adjusted at a speed of 540r / min and stirred for 5.3h; the condition for adjusting the pH value to neutral is to use a 0.1mol / L hydrochloric acid aqueous solution for adjustment; the washing condition is to use deionized water to wash until the filtrate is neutral;

[0055] A method for applying nanoparticle preparation technology in cosmetics, the application method comprising the following steps:

[0056] S1, mixing caprylic acid / capric acid triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water to obtain a mixed solution; wherein the weight ratio of caprylic acid / capric acid triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water is 70:0.53:0.23:1.38:10:35;

[0057] S2. Emulsifying the mixed solution, standing it for defoaming, and obtaining an emulsion; wherein the emulsification condition is emulsifying at a speed of 10000 r / min for 2 min.

[0058] Example 5

[0059] A nanoparticle preparation technology comprises the following steps:

[0060] 1) Stir corn starch and ammonia water evenly, perform ultrasonic treatment, freeze-dry, add sulfuric acid solution, stir and mix, let stand, filter, wash the solid, add deionized water, heat and mix, and obtain a suspension; wherein, the stirring time is 168 minutes; the ultrasonic treatment conditions are 40KHz frequency, 100W power, and ultrasonic treatment for 3 minutes; the stirring and mixing conditions are 39.6°C temperature, 190r / min speed, and stirring for 4.9 days; the washing conditions are washing with deionized water until neutral; the heating and mixing conditions are 54°C temperature and stirring for 28 minutes; the usage ratio of corn starch and ammonia water is 1.16g:8mL; the mass concentration of ammonia water is 1.44%; the concentration of sulfuric acid solution is 3.16mol / L; the usage ratio of corn starch, sulfuric acid solution and deionized water is 14.7g:100mL:17.6g;

[0061] 2) After mixing the suspension and octenyl succinic anhydride, adjusting the pH value and stirring the reaction, adjusting the pH value to neutral, centrifuging, washing, and obtaining modified starch; wherein the mass ratio of the suspension to octenyl succinic anhydride is 30:0.51; the condition for adjusting the pH value is to use a 3% mass concentration of sodium hydroxide aqueous solution to adjust the pH value to 8.74; the stirring reaction is adjusted at a speed of 570r / min and stirred for 5.4h; the condition for adjusting the pH value to neutral is to use a 0.1mol / L hydrochloric acid aqueous solution for adjustment; the washing condition is to use deionized water to wash until the filtrate is neutral;

[0062] A method for applying nanoparticle preparation technology in cosmetics, the application method comprising the following steps:

[0063] S1, mixing caprylic acid / capric acid triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water to obtain a mixed solution; wherein the weight ratio of caprylic acid / capric acid triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water is 70:0.54:0.24:1.44:10:35;

[0064] S2. Emulsifying the mixed solution, standing it for defoaming, and obtaining an emulsion; wherein the emulsification condition is emulsifying at a speed of 10000 r / min for 2 min.

[0065] Example 6

[0066] A nanoparticle preparation technology comprises the following steps:

[0067] 1) Stir corn starch and ammonia water evenly, perform ultrasonic treatment, freeze-dry, add sulfuric acid solution, stir and mix, let stand, filter, wash the solid, add deionized water, heat and mix, and obtain a suspension; wherein, the stirring time is 180 minutes; the ultrasonic treatment conditions are 40KHz frequency, 100W power, and ultrasonic treatment for 3 minutes; the stirring and mixing conditions are 40°C temperature, 200r / min speed, and stirring for 5.0d; the washing conditions are washing with deionized water until neutral; the heating and mixing conditions are stirring at 60°C temperature for 30 minutes; the usage ratio of corn starch and ammonia water is 1.2g:8mL; the mass concentration of ammonia water is 1.5%; the concentration of sulfuric acid solution is 3.16mol / L; the usage ratio of corn starch, sulfuric acid solution and deionized water is 14.7g:100mL:18g;

[0068] 2) After mixing the suspension and octenyl succinic anhydride, adjusting the pH value and stirring the reaction, adjusting the pH value to neutral, centrifuging, washing, and obtaining modified starch; wherein the mass ratio of the suspension to octenyl succinic anhydride is 30:0.52; the condition for adjusting the pH value is to use a 3% mass concentration of sodium hydroxide aqueous solution to adjust the pH value to 8.8; the stirring reaction is adjusted at a speed of 600r / min and stirred for 5.5h; the condition for adjusting the pH value to neutral is to use a 0.1mol / L hydrochloric acid aqueous solution for adjustment; the washing condition is to use deionized water to wash until the filtrate is neutral;

[0069] A method for applying nanoparticle preparation technology in cosmetics, the application method comprising the following steps:

[0070] S1, mixing caprylic acid / capric acid triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water to obtain a mixed solution; wherein the weight ratio of caprylic acid / capric acid triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water is 70:0.55:0.25:1.5:10:35;

[0071] S2. Emulsifying the mixed solution, standing it for defoaming, and obtaining an emulsion; wherein the emulsification condition is emulsifying at a speed of 10000 r / min for 2 min.

[0072] Comparative Example 1

[0073] Compared with Example 5, the difference is that no ammonia water is used in Comparative Example 1, and the other components, preparation steps and parameters are the same.

[0074] Comparative Example 2

[0075] Compared with Example 5, the difference is step 1);

[0076] Step 1) stir corn starch and ammonia water evenly, freeze-dry, add sulfuric acid solution, stir and mix, stand, filter, wash the solid, add deionized water and heat and mix to obtain a suspension; wherein the stirring time is 171 minutes; the stirring and mixing conditions are at a temperature of 39.6°C, a speed of 190r / min, and stirring for 4.9 days; the washing conditions are washing with deionized water until neutral; the heating and mixing conditions are at a temperature of 54°C and stirring for 28 minutes; the usage ratio of corn starch and ammonia water is 1.16g:8mL; the mass concentration of ammonia water is 1.44%; the concentration of sulfuric acid solution is 3.16mol / L; the usage ratio of corn starch, sulfuric acid solution and deionized water is 14.7g:100mL:17.6g;

[0077] The remaining components, preparation steps and parameters were the same.

[0078] Comparative Example 3

[0079] Compared with Example 5, the difference is that Comparative Example 3 does not use octenyl succinic anhydride, and the other components, preparation steps and parameters are the same.

[0080] Comparative Examples 4-6

[0081] Compared with Example 5, the difference is that the amounts of lupin protein, caffeic acid and modified starch in Comparative Examples 4-6 are as shown in Table 1, and the other components, preparation steps and parameters are the same.

[0082] Table 1 (Unit: parts by weight)

[0083] Lupin protein Caffeic acid Modified starch Example 5 0.54 0.24 1.44 Comparative Example 4 0 0.51 1.71 Comparative Example 5 0.66 0 1.56 Comparative Example 6 1.26 0.96 0

[0084] Different samples of Examples 1-6 and Comparative Examples 1-6 were stored at 55° C. for 15 days, and the astaxanthin retention rates therein were measured to evaluate the storage stability of the emulsion prepared by the present invention on astaxanthin. The test results are shown in Table 2.

[0085] The retention rate of astaxanthin is calculated as follows: retention rate of astaxanthin (%) = C 15 / C 0 ×100%;

[0086] Table 2

[0087] sample Astaxanthin retention rate (%) Example 1 89.4 Example 2 89.6 Example 3 89.9 Example 4 90.4 Example 5 90.9 Example 6 88.8 Comparative Example 1 79.4 Comparative Example 2 79.0 Comparative Example 3 70.7 Comparative Example 4 68.3 Comparative Example 5 65.4 Comparative Example 6 63.1

[0088] From the test results in Table 2, it can be seen that compared with Comparative Examples 1-6, the emulsions prepared in Examples 1-6 of the present invention have better stability, indicating that the presence of lupin protein, caffeic acid and modified starch in the emulsion can synergistically improve the storage stability of astaxanthin.

[0089] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A nanoparticle preparation technology, characterized in that: The following steps are involved: 1) Stir starch base and ammonia water evenly, perform ultrasonic treatment, freeze-dry, add sulfuric acid solution, stir and mix, let stand, filter, wash solid, add deionized water, heat and mix, and obtain a suspension; the dosage ratio of starch base and ammonia water is 1.0-1.2g:8mL; the mass concentration of ammonia water is 1.2-1.5%; the conditions of ultrasonic treatment are 40KHz frequency, 100W power, and ultrasonic treatment for 3min; 2) mixing the suspension and octenyl succinic anhydride, adjusting the pH value and stirring the reaction, adjusting the pH value to neutral, centrifuging, washing, and obtaining modified starch; The application method of the modified starch comprises the following steps: S1, mixing caprylic / capric triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water to obtain a mixed solution; S2. Emulsifying the mixed solution, standing it to defoam, and obtaining an emulsion.

2. A nanoparticle preparation technology according to claim 1, characterized in that: In step 1), the stirring time is 2-3 hours; the stirring and mixing conditions are 38-40°C temperature, 150-200r / min speed, and stirring for 4.5-5.0d; the washing conditions are washing with deionized water until neutral; the heating and mixing conditions are 30-60°C temperature and stirring for 20-30min.

3. A nanoparticle preparation technology according to claim 1, characterized in that: In step 1), the concentration of the sulfuric acid solution is 3.16 mol / L; the usage ratio of the starch base, the sulfuric acid solution and the deionized water is 14.7 g:100 mL:16-18 g.

4. The nanoparticle preparation technology according to claim 1, characterized in that: In step 2), the condition for adjusting the pH value is to adjust the pH value to 8.5-8.8 using a 3% mass concentration sodium hydroxide aqueous solution; the stirring reaction is adjusted to stir for 5.0-5.5 hours at a speed of 450-600 r / min; the condition for adjusting the pH value to neutrality is to adjust it using a 0.1 mol / L hydrochloric acid aqueous solution; the washing condition is to wash with deionized water until the filtrate is neutral.

5. The nanoparticle preparation technology according to claim 1, characterized in that: In step 2), the mass ratio of the suspension to octenyl succinic anhydride is 30:0.47-0.

52.

6. The nanoparticle preparation technology according to claim 1, characterized in that: In step S1, the weight ratio of caprylic / capric triglyceride, lupin protein, caffeic acid, modified starch, astaxanthin and deionized water is 70: 0.50-0.55: 0.20-0.25: 1.2-1.5: 10: 35; 7. The nanoparticle preparation technology according to claim 1, characterized in that: In step S2, the emulsification condition is at a rotation speed of 10000 r / min and emulsification for 2 min.