A sampling and picking structure of a chemiluminescent immunoassay analyzer

Abstract

The present invention discloses a sampling and picking structure of a chemiluminescent immunoanalyzer, which belongs to the field of immunoanalysis sampling, and includes an analysis and picking base and a sampling box. A sample storage mechanism is provided in the sampling box, which is used to provide a temporary storage space for mixing samples in the sampling box. A sampling and picking unit is provided on the top of the analysis and picking base, which is used to provide a driving force for sampling the sample in the sampling box and extracting the key components of the sample. After the original collected sample is injected into the sampling box, the present invention separates the sampling box from the external driving space by a closed cover, so as not to pollute the analysis environment, and directly transfers the original sample and the mixed composite magnetic particles internally by a quantitative sampling tube and a picking box, so as to avoid the use of the sampling tube causing the solution to adhere to the surface and reduce the accuracy of the analysis data. During this period, the plug is driven upward by a negative pressure to form a negative pressure space in the immunolabeling tank, so as to quantitatively extract the original sample in the original sample storage tank.

Description

Technical Field

[0001] The invention relates to the technical field of immunoassay sampling, and in particular to a sampling and picking structure of a chemiluminescent immunoassay analyzer. Background Art

[0002] Chemiluminescent immunoassay is a new type of labeled immunoassay technology that combines chemical reflection with immune reaction and is used to detect trace antigens or antibodies. According to the different reagents used, it is divided into: direct chemiluminescent immunoassay, chemiluminescent enzyme immunoassay, electrochemiluminescent immunoassay and photoinduced chemiluminescent immunoassay. Among them, electrochemiluminescent immunoassay has the advantages of long duration of luminescent reaction, high signal intensity, easy measurement and control, and has a wider application range than other analytical methods. Electrochemiluminescent immunoassay is to label antibodies (antigens) with electrochemiluminescent agent terpyridine ruthenium, use tripropylamine (TPA) as electron donor, and produce specific chemiluminescent reaction due to electron transfer in the electric field. Magnetic particle separation technology is used to adsorb magnetic particles coated with antibody-antigen to be tested-terpyridine ruthenium labeled antibody complex, and unbound labeled antibodies and specimens are washed away by buffer, and the magnetic particle solution after binding by electrode pressure undergoes electrochemiluminescent reaction;

[0003] At present, the sampling and picking method in the electrochemiluminescence immunoassay is: control the movement of the negative pressure tube through a multi-dimensional mobile module, then control the air pressure in the sampling tube through a negative pressure tube with adjustable air pressure, suck the sample to be tested into the sampling tube, and inject it into the labeled antigen (antibody) solution and the electrochemiluminescent agent in turn, during which the magnetic particles in the sampling tube are attracted by an electromagnet to achieve the transfer of the labeled complex, but when the sampling tube passes through different solution tubes, the solution will adhere to the surface of the sampling tube, causing the sample collection volume to be larger than the standard sampling value, and the solution hole is directly exposed to the air, which will pollute the external environment and pollute the next sampling and picking environment, affecting the subsequent electrochemiluminescence reaction. The numerical accuracy of the calculated antigen (antibody) to be tested based on the luminescence integral and the standard curve of the antigen (antibody) to be tested.

[0004] Therefore, we propose a sampling and picking structure for a chemiluminescent immunoassay analyzer. Summary of the invention

[0005] The purpose of the present invention is to solve the problem that in the existing electrochemiluminescence immunoassay sampling and picking method, when the sampling tube passes through different solution test tubes, the solution will adhere to the surface of the sampling tube, causing the sample collection amount to be larger than the standard sampling value, and the solution hole is directly exposed to the air, which will pollute the external environment and pollute the next sampling and picking environment. A sampling and picking structure of a chemiluminescence immunoassay is proposed.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A sampling and picking structure of a chemiluminescent immunoassay analyzer comprises an analysis and picking base and a sampling box, wherein the sampling box is placed on the top of the analysis and picking base, and a sample storage mechanism is arranged in the sampling box, wherein the sample storage mechanism is used to provide a temporary storage space for mixing samples in the sampling box;

[0008] A sampling and picking unit is arranged on the top of the analysis and picking base, and the sampling and picking unit consists of a sampling drive mechanism and a picking drive mechanism. The sampling drive mechanism is used to provide a driving force for sampling the sample in the sampling box, and the picking drive mechanism is used to provide a driving force for extracting key components of the sample in the sampling box.

[0009] Preferably, the sample storage mechanism includes an original sample storage tank, an immune labeling tank, a washing tank, a reaction substrate tank and an analysis and observation tank which are sequentially opened inside the sampling box in the order of sample mixing; the original sample storage tank and the immune labeling tank are isolated from each other, and the immune labeling tank, the washing tank, the reaction substrate tank and the analysis and observation tank are unidirectionally connected.

[0010] Preferably, a closed cover is fixed on the top of the sampling box, and the immunolabeling tank, washing tank, reaction substrate tank and analysis observation tank are connected to each other through a closed one-way slide groove.

[0011] Preferably, the sampling drive mechanism comprises a sampling placement truncated table arranged on the top of the analysis and picking base, a servo motor is arranged at the bottom of the sampling placement truncated table, and a groove is opened at the top of the sampling placement truncated table for stabilizing the bottom of the sampling box.

[0012] Preferably, the top of the sampling placement truncated table is movably connected to an original sampling extraction disc via a stabilizing column, the inner wall of the original sampling extraction disc is provided with a limiting arc-shaped slide groove undulating along the vertical direction, and a linear electromagnet group is radially arranged inside the original sampling extraction disc.

[0013] Preferably, a locking column that is engaged with the inner wall of the original sampling extraction disk is fixed on the outer surface of the stabilizing column, and a driving resistance arm that is magnetically attracted to the edge of the linear electromagnet group is rotatably connected to the top of the analysis pickup base.

[0014] Preferably, the picking drive mechanism includes a sampling closed hydraulic cylinder arranged inside the sampling placement table, a sampling closed ring is fixed to the top of the sampling closed hydraulic cylinder by a hydraulic connecting rod, a quantitative sampling tube is connected between the original sample storage slot and the immune labeling slot, the inner wall of the quantitative sampling tube is slidably connected to a sampling sliding rod driven to rise and fall by a limiting arc-shaped sliding groove, and a negative pressure driving plug is arranged at the bottom of the sampling sliding rod, and the bottom of the quantitative sampling tube is connected to a closed valve driven by a sampling closed ring.

[0015] Preferably, a pneumatically controlled resistor is provided on the top of the sampling placement truncated table, and the pneumatically controlled resistor consists of a pneumatic blocking cover slidably connected to the inner wall of the closed one-way slide groove, a pneumatic control cylinder fixed to the bottom of the sampling box, and a pneumatic driving cover slidably connected to the inner wall of the pneumatic control cylinder. A driving support bracket slidably connected to the top of the sampling closed circle is fixed to the bottom of the pneumatic driving cover, and the driving support bracket consists of support rods whose heights gradually increase toward the outside of the sampling placement truncated table.

[0016] Preferably, a pick-up box is slidably connected to the inner wall of the closed one-way slide groove, and the pick-up box is provided with a through groove along the tangential direction of the rotation of the sampling placement table. The inner wall of the closed one-way slide groove is provided with a limit slot for limiting the sliding position of the pick-up box. A lifting detector is provided at the bottom of the support rod, and the lifting detector is composed of a lifting detection sleeve slidably connected to the outer surface of the support rod, a support spring fixed to the bottom of the support rod and a pressure sensor arranged at the top of the sampling closed ring. When the pressure sensor detects an increase in pressure, the electromagnet at the corresponding position in the linear electromagnet group is turned on.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. In view of the problem that the solution hole slot in the sampling and picking method of the existing electrochemiluminescence immunoassay is directly exposed to the air and pollutes the analysis environment, and the problem that the solution adheres to the surface of the sampling tube when the sampling tube passes through different solution test tubes, a closing cover, a quantitative sampling tube and a picking box are provided to cooperate with each other. After the original collected sample is injected into the sampling box, the original sample storage slot is closed by the closing cover, and the sampling box is separated from the external driving space, so as not to pollute the analysis environment, and the original sample and the mixed composite magnetic particles are directly transferred internally through the quantitative sampling tube and the picking box, so as to avoid the solution adhering to the surface caused by the use of the sampling tube, thereby reducing the accuracy of the analysis data.

[0019] 2. By setting up the quantitative sampling tube, sampling sliding rod and negative pressure driving plug and other devices to cooperate with each other, the quantitative sampling tube is set to an inverted "U" shape, and the sampling sliding rod is driven by the rotation of the servo motor to slide on the inner wall of the limiting arc-shaped slide groove, driving the negative pressure driving plug to move upward, so that a negative pressure space is formed in the immune labeling groove, and the original sample in the original sample storage groove is quantitatively extracted. When the air pressure in the connecting space is the same, the closed valve is closed by the rising sampling closing ring to prevent the original sample from entering the immune analysis groove during the subsequent solution rotation and mixing process, thereby increasing the extraction amount of the original sample and causing deviations in the analysis data, thereby achieving the effect of accurately and quantitatively extracting the original sample.

[0020] 3. By setting up devices such as an air pressure blocking cover, an air pressure control cylinder and an air pressure driving cover to cooperate with each other, during the rising process of the sampling closed circle, the driving support bracket is driven to rise synchronously, and the support rod is driven to squeeze the air pressure blocking cover at the corresponding position on its top, thereby increasing the air pressure in the air pressure control cylinder and supporting the air pressure blocking cover upward to temporarily block the movement of the pick-up box. Then, when the rotation speed of the servo motor is increased, the blocking force is not sufficient to support the circular motion of the pick-up box with the previous radius, so that the pick-up box moves backward, thereby achieving a convenient control effect of controlling the movement of the pick-up box by controlling the servo motor. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is an exploded view of the overall structure of a sampling and picking structure of a chemiluminescent immunoassay analyzer proposed by the present invention;

[0022] Figure 2 A schematic diagram of the internal structure of the cross section of a sampling truncated table and an original sampling extraction disk of a sampling and picking structure of a chemiluminescent immunoassay analyzer proposed by the present invention;

[0023] Figure 3 For the present invention Figure 2 A magnified view of the structure at center A;

[0024] Figure 4 A schematic diagram of the coordination between a sampling closed circle and a driving support bracket structure of a sampling pickup structure of a chemiluminescent immunoassay analyzer proposed by the present invention;

[0025] Figure 5 An exploded view of a driving support bracket structure of a sampling and picking structure of a chemiluminescent immunoassay analyzer proposed by the present invention;

[0026] Figure 6 A schematic diagram of the internal structure of a sampling box cross section of a sampling and picking structure of a chemiluminescent immunoassay analyzer proposed by the present invention;

[0027] Figure 7 For the present invention Figure 6 A magnified view of the structure at B in the middle.

[0028] In the figure: 1. Analysis and picking base; 2. Sampling box; 21. Original sample storage tank; 22. Immunolabeling tank; 23. Washing tank; 24. Reaction substrate tank; 25. Analysis and observation tank; 26. Closing cover; 27. Closed one-way slide; 271. Limiting card slot; 3. Sampling placement round table; 31. Servo motor; 32. Placement slot; 33. Stable column; 331. Locking column; 34. Original sample extraction disk; 341. Limiting arc Slide; 342, linear electromagnet group; 35, drive resistance arm; 4, sampling closed hydraulic cylinder; 41, sampling closed ring; 5, quantitative sampling tube; 51, sampling sliding rod; 52, negative pressure drive plug; 53, closing valve; 6, air-controlled resistor; 61, air pressure blocking cover; 62, air pressure control cylinder; 7, drive support bracket; 71, support rod; 72, lifting detection sleeve; 73, support spring; 8, pick-up box; 81, through slot. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0030] Reference Figure 1-7 A sampling and picking structure of a chemiluminescence immunoassay analyzer includes an analysis and picking base 1 and a sampling box 2. The sampling box 2 is placed on the top of the analysis and picking base 1. A sample storage mechanism is arranged in the sampling box 2. The sample storage mechanism is used to provide a temporary storage space for mixing samples in the sampling box 2. A sampling and picking unit is arranged on the top of the analysis and picking base 1. The sampling and picking unit consists of a sampling drive mechanism and a picking drive mechanism. The sampling drive mechanism is used to provide a driving force for sampling samples in the sampling box 2. The picking drive mechanism is used to provide a driving force for extracting key components of the samples in the sampling box 2.

[0031] like Figure 5 and Figure 6 As shown, the sample storage mechanism includes an original sample storage tank 21, an immune labeling tank 22, a washing tank 23, a reaction substrate tank 24 and an analysis observation tank 25 which are sequentially opened inside the sampling box 2 according to the sample mixing order. The original sample storage tank 21 and the immune labeling tank 22 are isolated from each other, and the immune labeling tank 22, the washing tank 23, the reaction substrate tank 24 and the analysis observation tank 25 are unidirectionally connected through the picking box 8.

[0032] Through the above technical solution, the original sample storage tank 21 stores the original sample to be tested, the immune labeling tank 22 stores the labeled antigen-antibody solution, the washing tank 23 stores the washing buffer solution for the magnetic particles of the coated antibody-antigen to be tested-terpyridine ruthenium labeled antibody complex, the reaction substrate tank 24 stores the tripropylamine solution, and the analysis observation tank 25 is placed in an electric field to transfer electrons in the coated antibody-antigen to be tested-terpyridine ruthenium labeled antibody complex solution after mixing with tripropylamine, thereby generating a specific chemiluminescent reaction;

[0033] Based on the above, a closed cover 26 is fixed on the top of the sampling box 2 , and the immunolabeling tank 22 , the washing tank 23 , the reaction substrate tank 24 and the analysis and observation tank 25 are connected to each other through a closed one-way slide 27 .

[0034] Based on the above, before placing the sampling box 2 in the placement slot 32, the original sample is stored in the original sample storage slot 21, and then the closing cover 26 is closed on the top of the sampling box 2 to close the sampling box 2, and the original sample storage slot 21 is connected with the immune labeling slot 22 through the quantitative sampling tube 5.

[0035] like Figure 1 As shown, the sampling drive mechanism includes a sampling placement table 3 arranged on the top of the analysis and picking base 1, a servo motor 31 is arranged at the bottom of the sampling placement table 3, a placement groove 32 for stabilizing the bottom of the sampling box 2 is opened on the top of the sampling placement table 3, an original sampling extraction disk 34 is movably connected to the top of the sampling placement table 3 through a stabilizing column 33, a limiting arc-shaped slide groove 341 undulating along the vertical direction is opened on the inner wall of the original sampling extraction disk 34, and a linear electromagnet group 342 is radially arranged inside the original sampling extraction disk 34.

[0036] Through the above technical solution, a locking column 331 is fixed on the outer surface of the stabilizing column 33 and is engaged with the inner wall of the original sampling extraction disk 34, and a driving resistance arm 35 is rotatably connected to the top of the analysis and picking base 1 and is magnetically attracted to the edge of the linear electromagnet group 342, thereby providing rotational resistance to the rotation of the original sampling extraction disk 34, so that during the low-speed rotation of the sampling placement table 3, the original sampling extraction disk 34 and the sampling placement table 3 can rotate alternately first and then rotate synchronously.

[0037] Based on the above, the rotation speed of the servo motor 31 is divided into low-speed rotation and five-speed high-speed rotation. The first-speed high-speed rotation corresponds to the rotation speed of the original sample after mixing from the immune labeling tank 22 into the first washing tank 23, the second-speed high-speed rotation corresponds to the rotation speed of the complex magnetic particles entering the second washing tank 23 after preliminary washing, the third-speed high-speed rotation corresponds to the rotation speed of the complex magnetic particles entering the reaction substrate tank 24, i.e., the tripropylamine solution storage tank, after washing, and the fourth-speed high-speed rotation corresponds to the rotation speed of the mixed labeled sample solution after mixing the tripropylamine solution into the analysis and observation tank 25. After a period of time after reaching the fourth-speed rotation speed, the servo motor 31 is turned off to facilitate the observation of the chemiluminescence reaction in the analysis and observation tank 25.

[0038] like Figure 1 and Figure 2 As shown, the picking drive mechanism includes a sampling closed hydraulic cylinder 4 arranged inside the sampling placement truncated table 3, a sampling closed ring 41 is fixed to the top of the sampling closed hydraulic cylinder 4 through a hydraulic connecting rod, a quantitative sampling tube 5 is connected between the original sample storage tank 21 and the immune marker tank 22, a sampling sliding rod 51 driven to rise and fall through a limiting arc-shaped sliding groove 341 is slidably connected to the inner wall of the quantitative sampling tube 5, and a negative pressure driving plug 52 is arranged at the bottom of the sampling sliding rod 51, and a closed valve 53 driven by the sampling closed ring 41 is connected to the bottom of the quantitative sampling tube 5;

[0039] Through the above technical solution, the quantitative sampling tube 5 is set to an inverted "U" shape, and the sampling sliding rod 51 is set at the top of the immunolabeling groove 22. During the movement of the sampling sliding rod 51, a negative pressure space is formed inside the immunolabeling groove 22, and the original sample in the original sample storage groove 21 is sucked into the immunolabeling groove 22. After the negative pressure state in the immunolabeling groove 22 is released, the quantitative sampling tube 5 is closed by the closing valve 53, so that the original sample storage groove 21 enters the solution in the immunolabeling groove 22, and will not increase during the subsequent high-speed rotation process, thereby achieving the effect of quantitative sampling of the original sample;

[0040] Based on the above, after the top of the sampling box 2 is closed, the sampling box 2 is stabilized by placing it in the placement slot 32, and then the servo motor 31 is started to drive the sampling placement table 3 to rotate at a low speed, so that the sampling sliding rod 51 slides on the inner wall of the limiting arc-shaped sliding slot 341, driving the negative pressure driving plug 52 to slide upward, so that a negative pressure environment is formed in the immune labeling slot 22, and after the negative pressure driving plug 52 slides upward until the immune labeling slot 22 is connected to the original sample storage slot 21, the original sample is injected into the immune labeling slot 22 until the air pressure at both ends of the quantitative sampling tube 5 is the same, so that the original sampling sample is quantitatively transported to the immune labeling slot 22, completing the quantitative transfer operation of the original sampling sample, which is the initial sampling transfer process of the original sampling sample.

[0041] like Figure 5-7As shown, a pneumatically controlled resistor 6 is arranged on the top of the sampling placement truncated table 3, and the pneumatically controlled resistor 6 is composed of a pneumatic blocking cover 61 slidably connected to the inner wall of the closed one-way slide groove 27, a pneumatic control cylinder 62 fixed to the bottom of the sampling box 2, and a pneumatic driving cover slidably connected to the inner wall of the pneumatic control cylinder 62. A driving support bracket 7 slidably connected to the top of the sampling closed circle 41 is fixed to the bottom of the pneumatic driving cover, and the driving support bracket 7 is composed of support rods 71 ​​whose heights gradually increase toward the outside of the sampling placement truncated table 3, so that when the rotation speed of the servo motor 31 is increased, the blocking force of the pneumatic blocking cover 61 on the picking box 8 is increased, that is, the centripetal force on the picking box 8 during rotation is increased.

[0042] Through the above technical solution, the inner wall of the closed one-way slide 27 is slidably connected with the pick-up box 8, and the pick-up box 8 is provided with a through groove 81 along the tangential direction of the rotation of the sampling placement table 3. The inner wall of the closed one-way slide 27 is provided with a limit slot 271 for sliding the pick-up box 8. A lifting detector is provided at the bottom of the support rod 71. The lifting detector is composed of a lifting detection sleeve 72 slidably connected to the outer surface of the support rod 71, a support spring 73 fixed to the bottom of the support rod 71, and a pressure sensor provided at the top of the sampling closed ring 41. When the pressure sensor detects an increase in pressure, the electromagnet at the corresponding position in the linear electromagnet group 342 is turned on;

[0043] Based on the above, after the original sample is mixed with the solution of the immunolabeling tank 22 by rotating at a low speed for a period of time, the speed of the servo motor 31 is increased to a first-gear high-speed rotation, driving the pick-up box 8 located between the immunolabeling tank 22 and the washing tank 23 to slide and connect with the top of the air pressure blocking cover 61 under centrifugal motion, and rise to a first-gear high-speed process. At the top between the immunolabeling tank 22 and the washing tank 23, the electromagnet at the corresponding position of the linear electromagnet group 342 is turned on to attract the composite magnetic particles to the top of the pick-up box 8, and the key substance attached to the inner wall of the closed one-way slide 27, the composite magnetic particles, are scraped and pushed toward the next storage tank, i.e., the washing tank 23, through the movement of the pick-up box 8, and when the pick-up box 8 passes over the air pressure blocking cover 61, the electromagnet at its corresponding position is closed to complete the transfer operation of the key substance in the pick-up box 8. This is the extraction process of the composite magnetic particles;

[0044] Based on the above, in the subsequent washing process in the washing tank 23, the washing process of the composite magnetic particles is completed by gradually increasing the speed of the servo motor 31 to the third gear high speed, and then the tripropylamine solution in the reaction substrate tank 24 is used as the electron donor of the composite magnetic particles. After mixing, the speed of the servo motor 31 is increased to the fourth gear high speed, and the mixed pickup box 8 is moved to the analysis and observation tank 25 to wait for observation. This is the picking operation of the composite magnetic particles in the process of washing, substrate mixing and analysis and observation transfer;

[0045] Based on the above, a pressure sensor is further provided on the top of the sampling closed circle 41, which can identify the moving position of the picking box 8, thereby controlling the on and off of the electromagnet at the corresponding position, providing picking and positioning power, i.e. magnetic force, for the composite magnetic particles, and the magnetic force control is independent and does not affect each other, thereby ensuring the smooth progress of the magnetic particle separation process.

[0046] Before placing the sampling box 2 in the placement slot 32, the present invention stores the original sample in the original sample storage slot 21, and then closes the closing cover 26 on the top of the sampling box 2 to close the sampling box 2, and connects the original sample storage slot 21 with the immune labeling slot 22 through the quantitative sampling tube 5. This is the closing preparation process before sampling and picking up the original sample;

[0047] After the top of the sampling box 2 is closed, the sampling box 2 is stabilized by placing it in the placement slot 32, and then the servo motor 31 is started to drive the sampling placement table 3 to rotate at a low speed, so that the sampling sliding rod 51 slides on the inner wall of the limiting arc-shaped sliding slot 341, driving the negative pressure driving plug 52 to slide upward, so that a negative pressure environment is formed in the immune labeling slot 22, and after the negative pressure driving plug 52 slides upward until the immune labeling slot 22 is connected to the original sample storage slot 21, the original sample is injected into the immune labeling slot 22 until the air pressure at both ends of the quantitative sampling tube 5 is the same, so that the original sampling sample is quantitatively transported to the immune labeling slot 22, completing the quantitative transfer operation of the original sampling sample, which is the initial sampling transfer process of the original sampling sample;

[0048] Based on the above, the rotation speed of the servo motor 31 is divided into low-speed rotation and five-speed high-speed rotation. The first-speed high-speed rotation corresponds to the rotation speed of the original sample after mixing from the immune labeling tank 22 into the first washing tank 23. The second-speed high-speed rotation corresponds to the rotation speed of the composite magnetic particles after preliminary washing into the second washing tank 23. The third-speed high-speed rotation corresponds to the rotation speed of the composite magnetic particles after washing into the reaction substrate tank 24, i.e., the tripropylamine solution storage tank. The fourth-speed high-speed rotation corresponds to the rotation speed of the mixed labeled sample solution after mixing with the tripropylamine solution into the analysis and observation tank 25. After a period of time after reaching the fourth-speed rotation speed, the servo motor 31 is turned off to facilitate the observation of the chemiluminescence reaction in the analysis and observation tank 25.

[0049] Based on the above, the immune labeling tank 22 is further divided into a labeling antigen (antibody) tank and an antibody (antigen) tank, and a mixing hose is arranged between the two. In the initial placement process of the sampling box 2, through the squeezing block fixed to the sampling sliding rod 51 at the top of the mixing hose, when the sampling sliding rod 51 is not lifted and lowered by the limiting arc sliding groove 341, the inside of the immune labeling tank 22 close to the original sample storage tank 21 is in a negative pressure state, and the mixing hose is squeezed to make the mixing hose in a closed state, and the labeling antigen (antibody) tank is isolated from the antibody (antigen) tank. In the upward process of the sampling sliding rod 51, the closed state of the mixing hose is released, so that the labeled antibody (antigen) is mixed with the antigen (antibody), and the original sample is mixed with the solution in the immune labeling tank 22, so that the original sample forms a composite magnetic particle;

[0050] After the original sample is mixed with the solution of the immunolabeling tank 22 by rotating at a low speed for a period of time, the speed of the servo motor 31 is increased to the first gear high-speed rotation, driving the pick-up box 8 located between the immunolabeling tank 22 and the washing tank 23 to slide and connect with the top of the air pressure blocking cover 61 under centrifugal motion, and rise to the first gear high-speed process. At the top between the immunolabeling tank 22 and the washing tank 23, the electromagnet at the corresponding position of the linear electromagnet group 342 is turned on to attract the composite magnetic particles to the top of the pick-up box 8, and through the movement of the pick-up box 8, the key substance attached to the inner wall of the closed one-way slide 27 - the composite magnetic particles are scraped and pushed toward the next storage tank, i.e., the washing tank 23, and when the pick-up box 8 passes over the air pressure blocking cover 61, the electromagnet at its corresponding position is closed to complete the transfer operation of the key substance in the pick-up box 8. This is the extraction process of the composite magnetic particles.

[0051] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A sampling and picking structure of a chemiluminescent immunoassay analyzer, comprising an analysis and picking base (1) and a sampling box (2), wherein the sampling box (2) is placed on the top of the analysis and picking base (1), characterized in that: The sampling box (2) is provided with a sample storage mechanism, and the sample storage mechanism is used to provide a temporary storage space for mixing samples in the sampling box (2); A sampling and picking unit is arranged on the top of the analysis and picking base (1), and the sampling and picking unit is composed of a sampling drive mechanism and a picking drive mechanism, wherein the sampling drive mechanism is used to provide a driving force for sampling the sample in the sampling box (2), and the picking drive mechanism is used to provide a driving force for extracting key components of the sample in the sampling box (2); The sampling drive mechanism comprises a sampling placement truncated table (3) arranged on the top of the analysis and picking base (1), a servo motor (31) is arranged at the bottom of the sampling placement truncated table (3), a slot (32) for stably placing the bottom of the sampling box (2) is provided at the top of the sampling placement truncated table (3), an original sampling extraction disk (34) is movably connected to the top of the sampling placement truncated table (3) through a stabilizing column (33), a limiting arc-shaped sliding groove (341) undulating in the vertical direction is provided on the inner wall of the original sampling extraction disk (34), and a linear electromagnet group (342) is radially arranged inside the original sampling extraction disk (34); A locking column (331) is fixed on the outer surface of the stabilizing column (33) and is engaged with the inner wall of the original sampling extraction disk (34), and a driving resistance arm (35) is rotatably connected to the top of the analysis and picking base (1) and is magnetically attracted to the edge of the linear electromagnet group (342); The picking drive mechanism comprises a sampling closed hydraulic cylinder (4) arranged inside the sampling placement truncated table (3), a sampling closed ring (41) is fixed to the top of the sampling closed hydraulic cylinder (4) through a hydraulic connecting rod, an inverted U-shaped quantitative sampling tube (5) is arranged in the sampling box (2), the inner wall of the quantitative sampling tube (5) is slidably connected to a sampling sliding rod (51) driven to rise and fall through a limiting arc-shaped sliding groove (341), and a negative pressure driving plug (52) is arranged at the bottom of the sampling sliding rod (51), and the bottom of the quantitative sampling tube (5) is connected to a closed valve (53) driven by the sampling closed ring (41); A pneumatically controlled resistor (6) is arranged on the top of the sampling placement truncated table (3), and the pneumatically controlled resistor (6) is composed of a pneumatic blocking cover (61) slidably connected to the inner wall of the closed one-way slide groove (27), a pneumatic control cylinder (62) fixed to the bottom of the sampling box (2), and a pneumatic driving cover slidably connected to the inner wall of the pneumatic control cylinder (62), and a driving support bracket (7) slidably connected to the top of the sampling closed circle (41) is fixed to the bottom of the pneumatic driving cover, and the driving support bracket (7) is composed of support rods (71) whose heights gradually increase toward the outside of the sampling placement truncated table (3); The sampling box (2) is provided with a closed one-way slide groove (27), and a pick-up box (8) is slidably connected to the inner wall of the closed one-way slide groove (27). The pick-up box (8) is provided with a through groove (81) along the tangential direction of the rotation of the sampling placement table (3). The inner wall of the closed one-way slide groove (27) is provided with a limit slot (271) for sliding the pick-up box (8). A lifting detector is provided at the bottom of the support rod (71). The lifting detector consists of a lifting detection sleeve (72) slidably connected to the outer surface of the support rod (71), a support spring (73) fixed to the bottom of the support rod (71) and a pressure sensor arranged at the top of the sampling closed ring (41). When the pressure sensor detects an increase in pressure, the electromagnet at the corresponding position in the linear electromagnet group (342) is turned on.

2. The sampling and picking structure of a chemiluminescent immunoassay analyzer according to claim 1, characterized in that: The sample storage mechanism comprises an original sample storage tank (21), an immune labeling tank (22), a washing tank (23), a reaction substrate tank (24) and an analysis observation tank (25) which are sequentially opened inside the sampling box (2) according to the sample mixing sequence; the original sample storage tank (21) and the immune labeling tank (22) are isolated from each other, and the immune labeling tank (22), the washing tank (23), the reaction substrate tank (24) and the analysis observation tank (25) are unidirectionally connected.

3. The sampling and picking structure of a chemiluminescent immunoassay analyzer according to claim 2, characterized in that: A closed cover (26) is fixed on the top of the sampling box (2), and the immunolabeling tank (22), the washing tank (23), the reaction substrate tank (24) and the analysis and observation tank (25) are connected to each other via a closed one-way sliding groove (27).

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