A synergistic compound composition containing two active components and its application

Abstract

The present invention belongs to the field of pesticides and relates to a synergistic compound composition containing two active components. The composition contains two active components A and B, wherein component A is selected from the following structural compounds, and component B is selected from insecticides and miticides of the insect growth regulator class; the weight ratio of the two active components of component A and component B is 1:50 to 50:1; the composition of the present invention has an obvious synergistic effect and can be used to prevent and control a variety of pests and mites.

Description

Technical Field

[0001] The invention belongs to the field of pesticides and relates to a synergistic compound composition containing two active components and an application thereof. Background Art

[0002] In the actual process of agricultural production, the application of chemical agents is one of the most effective means of preventing and controlling plant diseases and insect pests. However, the most common problem caused by chemical pest control is the development of resistance of pests and mites. At the same time, the long-term continuous high-dose application of a single chemical insecticide and acaricide is likely to cause a series of problems such as agent residues and environmental pollution. Reasonable compounding or mixing of chemical insecticides and acaricides has the positive characteristics of expanding the insecticide and acaricide spectrum, improving the control effect, extending the appropriate period of application, reducing the amount of medicine used, reducing drug damage, reducing residues, and delaying the occurrence and development of pest and acaricide resistance. Compounding or mixing of insecticides and acaricides is one of the most effective ways to solve the above problems. The price of developing new pesticides continues to rise. In contrast, the development and research of high-efficiency, low-toxicity, and low-residue compounds and mixtures have low investment and short development cycle, and have attracted attention at home and abroad, and have increased their development efforts.

[0003] Insect growth regulators (IGRs) are a new type of insecticide that causes the death of pests by inhibiting insect growth and development (such as inhibiting molting, new cuticle formation, feeding, etc.). Different from traditional insecticides that act on the nervous system, IGRs have the characteristics of low toxicity, low pollution, and little impact on natural enemies and beneficial organisms. According to their mode of action and chemical structure, IGRs are mainly divided into juvenile hormone analogs, chitin synthesis inhibitors, and ecdysone analogs. IGRs are a type of specific insecticide that does not directly kill insects when used, but hinders or interferes with the normal development of insects during their individual development period, reducing the vitality of individual insects and causing their death. Common pesticides include diflubenzuron, cypermethrin, flubendiamide, and fenbuconazole.

[0004] Chinese invention patent CN112457288 discloses a piperic acid derivative and its application, wherein compound I-72 has contact and stomach poison activity and good conduction activity, and can be used to control a variety of Lepidoptera, Hemiptera, Thysanoptera, Coleoptera and other pests. The compound has the following structural formula:

[0005]

[0006] The combination of different ingredients may produce some unexpected technical effects and is also an effective method for preventing and controlling resistant pests. However, there is no report on the combination of compound I-72 and insect growth regulator pesticides. Summary of the invention

[0007] The purpose of the present invention is to provide a synergistic compound composition containing two active components and its application.

[0008] To achieve the above purpose, the technical solution adopted by the present invention is:

[0009] A synergistic composite composition containing two active components, the composition comprises two active components A and B, component A is selected from a piperic acid derivative compound of the following structure (i.e., compound I-72), and component B is selected from insecticides and acaricides of the insect growth regulator class; the weight ratio of the two active components of components A and B is 1:50 to 50:1; wherein the structural formula of the piperic acid derivative compound is as follows:

[0010]

[0011] The component B is a chitin synthesis inhibitor insecticide, a molting hormone insecticide or a juvenile hormone insecticide.

[0012] The chitin synthesis inhibitor insecticides: B1 buprofezin, B2 diflubenzuron, B3 hexaflumuron, B4 lufenuron, B5 flufenoxuron, B6 triflumuron, B7 chlorfluazuron, B8 teflubenzuron, B9 dichlorbenzuron, B10 fluazuron, B11 noviflumuron, B12 2 dichloroazithromyrin; B13 flucofuron; B14 flucycloxuron, B15 bistrifluron, B16 chlorbenzuron, B17 penfluron, B18 novaluron, B19 methotrexate, B20 thiazole, B21 azithromyrin, B22 diafenthiuron, B23 chloromethiuron, B24 diflovidazin, B25 cyromazine;

[0013] Molting hormone insecticides: B26 methoxyfenozide, B27 fufenozide, B28 furantebufenozide, B29 halofenozide, B30 chromafenozide, B31 tebufenozide, B32 fenprofenozide, B33 flometoquin, B34 protrifenbute;

[0014] Juvenile hormone insecticides: B35 pyriproxyfen, B36 hydroprene, B37 kinoprene, B38 methoprene, B39 triprene, B40 epofenonane, B41 epofenonane, B42 diofenolan, B43 diofenolan, B44 diofenolan, B45 precocene Ⅰ, B46 precocene Ⅱ, B47 precocene Ⅲ, B48 juvenilehormone Ⅰ, B49 juvenile hormone Ⅱ, B50 juvenile hormone Ⅲ.

[0015] Preferably, in the composition, component A is selected from the above-mentioned piperic acid derivative compounds; component B is selected from B1 buprofezin, B2 diflubenzuron, B3 hexaflumuron, B4 lufenuron, B5 flufenoxuron, B6 triflumuron, B7 chlorfluazuron, B16 chlorbenzuron, B22 diafenthiuron, B24 diflovidazin, B25 cyromazine, B26 methoxyfenozide, B27 fufenozide, B30 chromafenozide, B31 tebufenozide, B32 tebufenozide, B35 pyriproxyfen, and B38 methoprene;

[0016] The weight ratio of the two active components, component A and component B, is 1:20 to 20:1.

[0017] Further preferably, in the composition, component A is selected from the above-mentioned piperic acid derivative compounds; component B is selected from B1 buprofezin; B3 hexaflumuron; B4 lufenuron; B26 methoxyfenozide; B25 cyromazine;

[0018] The weight ratio of the two active components A and B is 1:10 to 10:1.

[0019] The application of the composition is used for preparing insecticides and miticides for preventing and controlling pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees and evergreen trees.

[0020] The composition is used for preventing or controlling pests of Lepidoptera, Hemiptera, Homoptera, Coleoptera, Diptera, Thysanoptera, Acarina, and their larvae and eggs.

[0021] The synergistic compound composition of the present invention has a significant synergistic effect in preventing and controlling pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, and evergreen trees. The results of indoor biological activity assays show that compound I-72 can be mixed with insect growth regulator insecticides and miticides to improve the control effect, reduce the dosage, and expand the insecticide spectrum. For example, when compound I-72 is mixed with thiamethoxam, hexaflumuron, lufenuron, methoxyfenozide, etc. in a ratio of 1:10 to 10:1, the activity against rice brown planthopper, striped stem borer, beet armyworm, and leafminer can be greatly improved; therefore, the synergistic compound composition of the present invention can be used for the preparation of insecticide drugs.

[0022] The synergistic compound composition of the present invention can be used to control pests, mites and underground pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, evergreen trees and the like; it can be particularly used to control piercing-sucking mouthpart pests and lepidopteran pests, such as planthoppers, whiteflies, aphids, thrips, leafhoppers, stink bugs, as well as striped stem borers, corn borers, diamondback moths, armyworms, leaf miners, cotton bollworms, beet armyworms and the like on a variety of crops.

[0023] In particular, the synergistic composite composition of the present invention is particularly useful for controlling Hemiptera pests, a non-exhaustive list of which includes, but is not limited to, Acrosternum hilare, green stink bug, Blissus leucopterus, chinch bug, Calocoris norvegicus, Cimex hemipterus, tropical bed bug, Cimex lectularius, bed bug, Dagbertus fasciatus, Dichelops furcatus, Dysdercus suturellus, cotton bug, Edessa meditabunda, Eurygaster maura, cereal bug, Euschistus neotropicalis heros), Euschistus servus, Brown stink bug, Helopeltis antonii, Helopeltis theivora, Tea horn stink bug, Lagynotomus spp., stink bugs, Leptocorisa oratorius, Leptocorisa varicornis, Lygus spp., Lygus, Lygus hesperus, Western grass stink bug, Maconellicoccus hirsutus, Neurocolpus longirostris, Nezara viridula, Southern green stink bug, Phytocoris spp., Lygus, California plant stink bug californicus), Phytocoris relativus, Piezodorus guildingi, Poecilocapsus lineatus, Four-lined stink bug, Psallus vaccinicola, Pseudacysta perseae, Scaptocoris castanea, and Triatoma spp., bloodsucking conenose bugs / kissing bugs.

[0024] In particular, the synergistic composite composition of the present invention is particularly useful for controlling homopteran pests (aphids, scale insects, whiteflies, planthoppers, leafhoppers), a non-exhaustive list of which includes, but is not limited to, pea aphids (Acrythosiphon pisum), pea aphids, Adelges spp., aphids, cabbage whitefly (Aleurodes proletella), cabbage whitefly, spiral whitefly (Aleurodicus disperses), soft hair whitefly (Aleurothrixus floccosus), cotton whitefly, Aluacaspis spp., Amrasca bigutella bigutella, Aphrophora spp., leafhoppers, Aonidiella aurantii, Aphis spp., aphids of Aphis spp., cotton aphids (Aphis gossypii), cotton aphids, apple aphids (Aphis pomi), apple aphid, Aulacorthum solani, foxglove aphid, Bemisia spp., whitefly, Bemisia argentifolii, Bemisia tabaci, sweet potato whitefly, Brachycolus noxius, Russian aphid, Brachycorynella asparagi, asparagus aphid, Brevennia rehi, Brevicoryne brassicae, cabbage aphid, Ceroplastes spp. scale insects, Ceroplastes rubens, red wax scale insects, Chionaspis spp. scale insects, Chrysomphalus spp.), scale insects of the genus Coccus, Dysaphis plantaginea, pink apple aphid, leafhoppers of the genus Empoasca, woolly apple aphids Eriosoma lanigerum, woolly apple aphids, Icerya purchasi, woolly scale insects, Idioscopus nitidulus, mango leafhoppers, Laodelphax striatellus, brown planthoppers, Lepidosaphes, Macrosiphum.), potato aphid (Macrosiphum euphorbiae), potato aphid, wheat aphid (Macrosiphum granarium), English wheat aphid, rose aphid (Macrosiphum rosae), rose aphid, aster leafhopper (Macrosteles quadrilineatus), aster leafhopper, raspberry foamhopper (Mahanarva frimbiolata), wheat aphid (Metopolophium dirhodum), rose wheat aphid, long-horned bug (Mictis longicornis), peach aphid (Myzus persicae), green peach aphid, leafhoppers of the genus Nephotettix (Nephotettix spp.), black-tailed leafhopper (Nephotettix cinctipes), green leafhopper, green leafhopper, rice brown planthopper (Nilaparvata lugens), brown planthopper, bran shield scale (Parlatoria pergandii), bran scale, black scale scale (Parlatoria ziziphi), ebony scale, corn planthopper (Peregrinus maidis), corn flower-winged planthopper, Philaenus spp.'s scale, grape phylloxera (Phylloxera vitifoliae), grape phylloxera, spruce scale (Physokermes piceae), spruce round scale, planococcus spp., mealybugs, mealybugs of Pseudococcus spp., pineapple mealybug (Pseudococcus brevipes), pineapple mealybug, pear round scale (Quadraspidiotus perniciosus), St. Joseph's scale, Rhapalosiphum spp.), corn leaf aphids (Rhapalosiphummaida), corn aphids, grain aphids (Rhapalosiphum padi), grain aphids, scale insects of the genus Saissetia spp., olive black helmet scale insects (Saissetia oleae), black scale insects, Schizaphis graminum, green aphids, wheat long-tube aphids (Sitobion avenae), English wheat aphids, white-backed rice hoppers (Sogatella furcifera), white-backed planthoppers, aphids of the genus Therioaphis spp., scale insects of the genus Toumeyella spp., aphids of the genus Toxoptera spp., whiteflies (Trialeurodes spp.), greenhouse whitefly (Trialeurodes vaporariorum), greenhouse whitefly, green hemp whitefly (Trialeurodes abutiloneus), winged whitefly, scale insects of the genus Unaspis spp., Unaspis yanonensis, arrowhead scale insects, and Zulia entreriana.

[0025] In particular, the synergistic combination composition of the present invention is particularly useful for controlling Thysanoptera pests (thrips), a non-exhaustive list of which includes, but is not limited to, Frankliniella fusca, Tobacco thrips, Frankliniella occidentalis, Western flower thrips, Frankliniella shultzei, Frankliniella williamsi, Corn thrips, Heliothrips haemorrhaidalis, Greenhouse thrips, Riphiphorothrips cruentatus, Scirtothrips spp., Scirtothrips citri, Citrus thrips, Scirtothrips dorsalis, Scirtothrips dorsalis, Taeniothrips rhopalantennalis, and Thrips spp.

[0026] In particular, the synergistic composite composition of the present invention is particularly useful for controlling coleopteran (beetle) pests, a non-exhaustive list of which includes, but is not limited to: Acanthoscelides spp., weevils, Acanthoscelides obtectus, soybean weevils, Agrilus planipennis, ash borers, Agriotes spp., wireworms, Anoplophora glabripennis, Asian longhorn beetles, Anthonomus spp., weevils, Anthonomus grandis, boll weevils, Aphidius spp., Apion spp., weevils, Apogonia spp., grubs, Ataenius spretulus, turfgrass black beetle, beet cryptic beetle (Atomaria linearis), small beet beetle, melon beetle species (Aulacophora spp.), beet weevil (Bothynoderes punctiventris), beet root weevil, bean weevil species (Bruchus spp.), pea weevil (Bruchus pisorum), pea weevil, Cacoesia species, four-striped bean weevil (Callosobruchusmaculatus), southern cowpea weevil, yellow-spotted dew beetle (Carpophilus hemipteras), dried fruit beetle, beet tortoise beetle (Cassida vittata), Cerosterna species, Cerotoma species, leaf beetle, bean leaf beetle (Cerotomatrifurcata), bean leaf beetle, tortoise beetle species (Ceutorhynchus spp.), cabbage weevil (Ceutorhynchus assimilis), cabbage heart weevil, citrus weevil (Ceutorhynchus napi), cabbage weevil, Chaetocnema spp., Colaspis spp.), soil beetles, Conoderus scalaris, Conoderus stigmosus, Conotrachelus nenuphar, plum cone weevil, Cotinus nitidis, green flower beetle, Crioceris asparagi, asparagus leaf beetle, Cryptolestes ferrugineus, rusty flat flour beetle, Cryptolestes pusillus, flat flour beetle, Turkish flat flour beetle (Cryptolestesturcicus), Turkish flour beetle, Ctenicera spp., Curculio spp., Cyclocephala spp., Cylindrocpturus adspersus, sunflower stem weevil, Deporaus marginatus), mango leaf cutter weevil, Dermesteslardarius, ham weevil, Dermestes maculates, hidden beetle, Diabrotica spp. chrysolemid, Epilachna varivestis, Mexican bean beetle, Faustinus cubae, pale bark weevil, aggressive weevil, Hypera spp., Hypera postica, clover weevil, Hyperdoes spp., Argentine stem weevil, Hypothenemus hampei, coffee berry beetle, Ips spp., engraver beetle, Lasioderma serricorne), tobacco beetle, potato beetle (Leptinotarsa ​​decemlineata), Colorado potato beetle, Liogenys fuscus, Liogenys suturalis, Lissorhoptrus oryzophilus, rice water weevil, Lyctus spp., wood beetle, powder beetle, Maecolaspis joliveti, Megascelis spp., corn beetle (Melanotus communis), cauliflower beetle (Meligethes spp.), Meligethes aeneus, Flower beetles, Melolontha melolontha, Common European beetle, Oberea brevis, Oberea linearis, Oryctes rhinoceros, Date palm beetle, Oryzaephilus mercator, Market saw-toothed grain beetle, Oryzaephilus surinamensis, Saw-toothed grain beetle, Otiorhynchus spp., Oulema melanopus, Grain leaf beetle, Oulema oryzae, Pantomorus spp., Phyllophaga spp., May / June beetle, June beetle cuyabana), Phyllotreta spp., Phyllotretavittuta fabr., Yellow curvy flea beetle, Yellow straight flea beetle, Yellow narrow flea beetle, Yellow wide flea beetle, Leaf beetle, Phynchites spp., Popillia japonica, Japanese beetle, Prostephanus truncates, Rhizopertha dominica, Rhizopertha dominica, Rhizotrogus spp., European beetle, Rhynchophorus spp., Scolytus spp., Wood beetle), Shenophorus spp., Sitonalineatus, Pea leaf weevil, Sitophilus spp. spp.), cereal weevils, grain weevils (Sitophilusgranaries), grain worms, rice weevils (Sitophilus oryzae), rice weevils, small round skin beetles (Stegobiumpaniceum), stealing beetles, Tribolium spp., flour beetles, red flour beetles (Triboliumcastaneum), red flour beetles, mixed flour beetles (Triboliumconfusum), mixed flour beetles, spotted skin beetles (Trogodermavariabile), warehouse beetles and greedy carabids (Zabrustenebioides).

[0027] In particular, the synergistic combination composition of the present invention is particularly useful for controlling lepidopteran pests, a non-exhaustive list of which includes, but is not limited to: Pyralidae, such as Chilosuppressalis (Walker), Tryporyzaincertulas (Walker), Cnaphalocrocis medinalis Guenee, Hellullaundalis, Conogethes punctiferlis; Pieridae, such as Pieris rapae, Papilio xuthus, Pierisrapaecrucivora, Parnara guttata; Arctiidae, such as Hyphantria nymphae, cunea); Noctuidae, such as trichoplusiani, mamestra brassicae, spodoptera exigua, spodoptera litura, helicoverpaarmigera, Pseudaletia separate, Agrotis ipsilon; Tortricidae, such as adoxophyes orana fasciata, archipsfuscocureanus, homona magnanima; Plutellidae, such as plutella xylotella; Gelechiidae, such as pectinophoragossypiella, Pink bollworm, etc.

[0028] In particular, the synergistic compound composition of the present invention can be used to control citrus panonychus mite, citrus rust mite, citrus early spider mite, hawthorn spider mite, apple panonychus mite, two-spotted spider mite, tea yellow mite, tea orange gall mite, tea short-palped mite, tea gall mite, cinnabarinus spider mite, truncate spider mite, Turkestan spider mite, Dunhuang spider mite, wheat round red spider, wheat long-legged spider, jujube tick, citrus leaf miner, citrus scale insect, fruit tree leaf miner, Fruit borer, soybean borer, golden moth, tea geometrid, tea caterpillar, tea green leafhopper, pear psyllid, rice planthopper, greenhouse whitefly, American leafminer, yellow-striped flea beetle, striped stem borer, yellow stem borer, corn borer, bean pod borer, melon borer, rice water weevil, cotton bollworm, pink bollworm, fruit aphid, soybean aphid, vegetable aphid, wheat aphid, cotton aphid, diamondback moth, armyworm, cabbage worm, tobacco budworm, longhorn beetle and other pests and mites.

[0029] The synergistic compound composition of the present invention can be applied at an effective dose to the pests / mites to be controlled and / or their habitats. Therefore, the synergistic compound composition of the present invention provides a method for controlling or preventing plant pests.

[0030] The active ingredients in the synergistic compound composition of the present invention can be applied to pests, plants and their habitats simultaneously (for example, as a preformulated mixture or tank mix) or in a suitable sequence.

[0031] Advantages of the present invention: The binary composition obtained by compounding compound I-72 with insect growth regulator insecticides and miticides in an appropriate ratio has a good synergistic effect, thereby improving the prevention effect, reducing the dosage, and expanding the insecticide spectrum. Specifically, 1. The compounded two active components A and B have different mechanisms of action, which can delay the development of drug resistance in pests and mites. 2. The insecticide spectrum is expanded and can be used to prevent and control various pests and mites on various crops such as fruit trees, vegetables, ornamental plants, tea, and cotton; 3. The amount of pesticide used is reduced, the amount of pesticide residues on crops is reduced, and the friendliness to the environment is enhanced. 4. The two active components A and B are compounded within an appropriate ratio range to improve the control effect of pests and mites such as rice brown planthoppers, striped stem borers, beet armyworms, and leafminers. DETAILED DESCRIPTION

[0032] The following examples are used to explain the present invention in detail, so as to facilitate further understanding of the present invention. The active component A is selected from compound I-72 (prepared according to the description in the patent document CN112457288, with a content of 97%); the active component B and other raw materials are all commercially available products, and each component is measured by weight percentage.

[0033] Example 1: Indoor synergistic screening test of compound I-72 mixed with different chitin synthesis inhibitors against Plutella xylostella

[0034] Test target: 2nd instar larvae of diamondback moth (Plutella xylostella).

[0035] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0036] Experimental method: First, select cabbage leaves, and immerse them in the prepared solution for 10 seconds in the order from low dose to high dose according to the experimental design. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate neatly packed healthy test insects. Each treatment has 10 heads and each treatment is repeated 3 times. A blank control (CK) is also set up.

[0037] The treated test materials were placed in an observation room where the temperature, humidity and light intensity could be adjusted as needed. The number of dead and live insects was investigated after 72 hours and the corrected mortality rate was calculated using the Abbott formula.

[0038] The Bliss method was used to evaluate the synergistic effect of mixed pesticides. Based on the concept of independent combined effects proposed by Bliss, he believed that the theoretical mortality rate P of mixed pesticides can be calculated by the following formula:

[0039] P=Pm+Pn(1-Pm)

[0040] Pm is the measured mortality rate (%) of the target when the concentration of the first active component is m; Pn is the measured mortality rate (%) of the target when the concentration of the second active component is n.

[0041] If the actual mortality rate of the target after the two active components are mixed at a certain concentration is greater than the theoretical mortality rate P, it is determined that the two active components have a synergistic effect when mixed at the set concentration, otherwise it is an antagonistic effect. The test results are shown in Table 1.

[0042] Table 1 Results of indoor synergistic screening of compound I-72 and different chitin synthesis inhibitors against Plutella xylostella

[0043]

[0044]

[0045] Example 2: Indoor synergistic screening test of compound I-72 mixed with different ecdysone insecticides and juvenile hormone insecticides on corn borer

[0046] Test target: Test target: 3rd instar larvae of corn borer (Ostrinia furnacnlis Guenee).

[0047] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0048] Experimental method: First, select corn tender stems, and immerse them in the prepared solution for 10 seconds in the order from low dose to high dose according to the experimental design. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate neatly arranged healthy test insects. Each treatment has 10 heads, and each treatment is repeated 3 times. A blank control (CK) is also set up.

[0049] The treated test materials were placed in an observation room where the temperature, humidity and light intensity could be adjusted as needed. The number of dead and live insects was investigated after 72 hours and the corrected mortality rate was calculated using the Abbott formula.

[0050] The synergistic effect of the mixture was evaluated by the Bliss method, which was the same as in Example 1. The test results are shown in Table 2.

[0051] Table 2 Results of indoor synergistic screening of compound I-72 mixed with different ecdysone insecticides and juvenile hormone insecticides against corn borer

[0052]

[0053]

[0054] Example 3: Indoor joint toxicity test of compound I-72 and buprofezin against rice brown planthopper

[0055] Test target: Rice brown planthopper [Nilaparvata lugens (Stal)] nymphs, a susceptible strain reared indoors.

[0056] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0057] Test method: The pot seedling spray method was used to determine the activity against rice brown planthopper nymphs. First, rice seedlings were planted in a culture pot and sprayed evenly in the order from low dose to high dose according to the experimental design, 2 mL per plant, and then rice brown planthopper nymphs of the same size were inoculated on the rice seedlings. Each treatment was repeated 3 times, and a blank control was set up.

[0058] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture.50 Values and 95% confidence limits were determined, and the co-toxicity coefficient of each formulation was calculated using the Sun Y-p method to evaluate the combined effect. The test results are shown in Table 3.

[0059] The co-toxicity coefficient (CTC value) of the mixture was calculated according to the following formula:

[0060]

[0061] Where: ATI - the actual toxicity index of the mixture; S - the LC 50 ; M - the LC 50 .

[0062] TTI = TIA × PA + TIB × PB

[0063] Where: TTI - the theoretical toxicity index of the mixture; TIA - the toxicity index of agent A; PA - the percentage content of agent A in the mixture; TIB - the toxicity index of agent B; PB - the percentage content of agent B in the mixture.

[0064]

[0065] Where: CTC - co-toxicity coefficient; ATI - the actual toxicity index of the mixture; TTI - the theoretical toxicity index of the mixture.

[0066] The co-toxicity coefficient (CTC) of the compound mixture: CTC ≥ 120 indicates synergistic effect; 80 < CTC < 120 indicates additive effect; CTC ≤ 80 indicates antagonistic effect.

[0067] Table 3 Results of indoor combined toxicity determination of compound I-72 and buprofezin against Nilaparvata lugens

[0068]

[0069] Example 4: Indoor combined toxicity determination test of compound I-72 and tebufenozide against Chilo suppressalis

[0070] Test target: 3rd instar larvae of Chilo suppressalis (Walker).

[0071] Preparation of liquid medicine: According to different test requirements, the test samples were accurately weighed separately using an electronic analytical balance. The technical materials were dissolved in acetone and then diluted with 0.1% Tween 80 aqueous solution to a series of liquid medicines with a certain concentration gradient according to the test design dose.

[0072] It should be noted that in the original text, there is an error in the description of the additive effect range in item . It should be "80 < CTC < 120" instead of "120 < CTC < 120" as it is currently written. The translation has been corrected accordingly.Experimental method: First, select tender rice stems and immerse them in the prepared solution for 10 seconds in the order from low dose to high dose according to the experimental design. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate neatly arranged healthy test insects. Each treatment has 10 heads and each treatment is repeated 3 times. A blank control is also set up.

[0073] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 3). The test results are shown in Table 4.

[0074] Table 4 Results of indoor combined toxicity test of compound I-72 and methoxyfenozide against Chilo suppressalis

[0075]

[0076] Example 5: Indoor combined toxicity test of compound I-72 and lufenuron against Spodoptera exigua

[0077] Test target: 2nd instar larvae of beet armyworm (Spodoptera exigua Hübner).

[0078] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0079] Experimental method: First, select cabbage leaves, and immerse them in the prepared solution for 10 seconds in the order from low dose to high dose according to the experimental design. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate neatly packed healthy test insects. Each treatment has 10 heads, and each treatment is repeated 3 times. A blank control is also set up.

[0080] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The co-toxicity coefficient of each ratio was calculated by Sun Yunpei's method to evaluate the mixing effect (same as Example 3). The test results are shown in Table 5.

[0081] Table 5 Results of indoor combined toxicity test of compound I-72 and lufenuron against Spodoptera exigua

[0082]

[0083] Example 6: Indoor combined toxicity test of compound I-72 and cyromazine against American leafminer

[0084] Test target: American leafminer (Liriomyza sativae Blanchard) larvae.

[0085] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0086] Test method: First, select bean seedlings carrying leafminer larvae (larvae have just invaded and formed worm tunnels), and spray them in the order from low dose to high dose according to the test design. Each treatment is repeated 3 times, and a blank control is set up.

[0087] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After the blank control had pupated, the number of dead and live insects was checked, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations and LC values ​​for each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 3). The test results are shown in Table 6.

[0088] Table 6 Results of indoor combined toxicity test of compound I-72 and cyromazine against American leafminer

[0089]

[0090]

[0091] Example 7: Indoor combined toxicity test of compound I-72 and hexaflumuron against armyworms

[0092] Test target: 2nd instar larvae of armyworm (Mythimna seperata).

[0093] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0094] Experimental method: First, select corn leaves and immerse them in the prepared solution for 10 seconds in the order from low dose to high dose according to the experimental design. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate neatly packed healthy test insects. Each treatment has 10 heads and each treatment is repeated 3 times. A blank control is also set up.

[0095] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 3). The test results are shown in Table 7.

[0096] Table 7 Results of indoor combined toxicity test of compound I-72 and hexaflumuron against armyworms

[0097]

[0098] The description of the above embodiments is only used to help understand the method of the present invention and its core idea. It should be pointed out that for ordinary technicians in this technical field, without departing from the principle of the present invention, the present invention can also be improved and modified in several ways, and these improvements and modifications also fall within the protection scope of the claims of the present invention. It can be seen from the above technical scheme that the insecticide and acaricide composition of the present invention has the advantages of improving drug efficacy, expanding insecticide spectrum, reducing the amount of pesticides used, reducing the amount of pesticide residues on crops, reducing environmental pollution, being safe for humans and animals, having good environmental compatibility, and pests are not easy to develop drug resistance. The composition of the present invention can be applied to the prevention and control of various pests and mites of various crops, and can be widely used in the field of agriculture.

Claims

1. A synergistic composite composition containing two active ingredients, characterized in that: The composition comprises two active components A and B, wherein component A is selected from the following piperic acid derivative compounds, and component B is selected from B1 buprofezin, B2 diflubenzuron, B3 hexaflumuron, B4 lufenuron, B5 flufenoxuron, B6 triflumuron, B7 chlorfluazuron, B16 chlorbenzuron, B22 diafenthiuron, B25 cyromazine, B26 methoxyfenozide, B27 fufenozide, B31 tebufenozide, B35 pyriproxyfen, and B38 methoprene; the weight ratio of the two active components A and B is 1:50 to 50:1; wherein the structural formula of the piperic acid derivative compound is as follows: 。 2. The composition according to claim 1, characterized in that: The weight ratio of the two active components, component A and component B, is 1:20 to 20:

1.

3. The composition according to claim 2, characterized in that: Component A in the composition is selected from the piperic acid derivative compounds; component B is selected from B1 buprofezin; B3 hexaflumuron; B4 lufenuron; B26 methoxyfenozide; B25 cyromazine; the weight ratio of the two active components A and B is 1:10 to 10:

1.

4. The use of the composition according to claim 1, characterized in that: The composition is used for preparing insecticides for preventing and controlling brown planthopper, American leafminer, diamondback moth, pyralids and noctuid pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees and evergreen trees.

Images