A synergistic compound composition containing spirocyclic quaternary acid insecticides and acaricides and its application

Abstract

The present invention belongs to the field of pesticides, and relates to a synergistic compound composition containing two active components. The composition contains two active components A and B, wherein component A is selected from the following structural compounds, and component B is selected from spirodiclofen, spirotetramat, spiromesifen, spirotetramat, spiropidion, spirobamate, and spidoxamat; the weight ratio of the two active components A and B is 1:50 to 50:1; the composition of the present invention has an obvious synergistic effect, and can be used to prevent and control a variety of pests and mites.

Description

Technical Field

[0001] The invention belongs to the field of pesticides and relates to a spirocyclic quaternary acid insecticide and acaricide synergistic compound composition and application thereof. Background Art

[0002] In the actual process of agricultural production, the application of chemical agents is one of the most effective means of preventing and controlling plant diseases and insect pests. However, the most common problem caused by chemical pest control is the development of resistance of pests and mites. At the same time, the long-term continuous high-dose application of a single chemical insecticide or miticide is likely to cause a series of problems such as agent residues and environmental pollution. Reasonable compounding or mixing of chemical insecticides and miticides has the positive characteristics of expanding the insecticide and acaricide spectrum, improving the control effect, extending the appropriate period of application, reducing the amount of medicine used, reducing drug damage, reducing residues, and delaying the occurrence and development of pest and acaricide resistance. Compounding or mixing of insecticides and miticides is one of the most effective ways to solve the above problems.

[0003] The insecticidal and acaricidal mechanism of spirocyclic tert-acid compounds is completely different from the existing insecticides and acaricides. It inhibits lipid synthesis in the pests, destroys the energy metabolism of the pests, and ultimately kills the pests and mites. Spirodiclofen belongs to the tert-acid class and is a highly efficient and low-toxic acaricide with a completely new mechanism of action. It does not have the problem of cross-resistance with existing acaricides and has a good control effect on citrus mites. Spirotetramat is a modern insecticide with bidirectional systemic conduction properties. At the end of 2007, spirotetramat was approved for the first registration in the world in Tunisia. In 2008, Bayer registered and marketed spirotetramat in the United States and Canada under the trade name Movento. On September 7, 2012, Bayer CropScience (China) Co., Ltd. obtained the formal registration of 22.4% spirotetramat SC in China. The preparation product is registered for the control of tomato whiteflies and citrus scale insects. Spiromesifen is a spirocyclic quaternary ketoacid insecticide and acaricide developed by Bayer. Bayer CropScience first registered it in the UK in 2003. It is mainly used for cotton, vegetables and ornamental plants to control whiteflies and spider mites. Spiropidion is the fourth spirocyclic quaternary ketoacid insecticide developed and marketed by Syngenta after Bayer launched spiromesifen, spiromesifen and spirotetramat. The molecule contains a keto-enol tautomerization structure, and its chemical structure is extremely similar to spirotetramat. Spiromethafen is a new compound synthesized on the basis of studying the structure of the high-efficiency isomer of spiromesifen. It has different groups and higher activity. Currently, only Zhejiang Yulong Biotechnology Co., Ltd. has obtained the exclusive registration of spiromethafen in China.

[0004] Chinese invention patent CN112457288 discloses a piperic acid derivative and its application, wherein compound I-72 has contact and stomach poison activity and good conduction activity, and can be used to control a variety of Lepidoptera, Hemiptera, Thysanoptera, Coleoptera and other pests. The compound has the following structural formula:

[0005]

[0006] The compounding of different varieties of ingredients may produce some unexpected technical effects and is also one of the effective methods for preventing and controlling resistant pests and mites. However, there is no report on the compounding composition of compound I-72 and tetronic acid insecticides and miticides. Summary of the invention

[0007] The purpose of the present invention is to provide a spirocyclic quaternary acid insecticide and acaricide synergistic compound composition and application.

[0008] To achieve the above purpose, the technical solution adopted by the present invention is:

[0009] A spirocyclic tetronic acid insecticide and acaricide synergistic composite composition, the composition comprises two active components A and B, component A is selected from a piperonic acid derivative compound of the following structure (i.e., compound I-72), and component B is selected from a tetronic acid insecticide and acaricide; the weight ratio of the two active components A and B is 1:50 to 50:1; wherein the piperonic acid derivative compound has the following structural formula:

[0010]

[0011] The tetronic acid insecticide and acaricide is selected from: B1 spirodiclofen, B2 spirotetramat, B3 spiromesifen, B4 spirotetramat, B5 spiropidion, B6 spirodiclofen or B7 spidoxamat.

[0012] Preferably, in the composition, component A is selected from piperic acid derivative compounds; component B is selected from B1 spirodiclofen, B2 spirotetramat, B3 spiromesifen, B4 spirotetramat, B5 spiropidion, B6 spirobamate or B7 spidoxamat;

[0013] The weight ratio of the two active components, component A and component B, is 1:20 to 20:1.

[0014] Further preferably, in the composition, component A is selected from piperic acid derivative compounds; component B is selected from B1 spirodiclofen, B2 spirotetramat, B3 spiromesifen, B5 spiropidion or B6 spirodiclofen;

[0015] The weight ratio of the two active components A and B is 1:10 to 10:1.

[0016] The application of the composition is used for preparing insecticides and miticides for preventing and controlling pests, mites or urban health pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, evergreen trees.

[0017] The composition is used for preventing or controlling pests of Hemiptera, Homoptera, Lepidoptera, Coleoptera, Diptera, Thysanoptera, Acarina and their larvae and eggs.

[0018] The insecticidal and acaricidal composition of the present invention has a significant synergistic effect in preventing and controlling pests, mites or urban health pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, evergreen trees. The results of indoor biological activity assays show that compound I72 can be mixed with spirodiclofen, spirotetramat, spiromesifen, ethyl methoxypiperidion, and spirodiclofen to improve the prevention effect, reduce the dosage, and expand the insecticide spectrum. For example, when component A is mixed with spirodiclofen, spirotetramat, spiromesifen, ethyl methoxypiperidion, and spirodiclofen in a ratio range of 1:10 to 10:1, the activity against targets such as cinnabarinus spider mite, cotton aphid, yellow thrips, and whitefly can be greatly improved; therefore, the two active component compositions of the present invention can be used for the preparation of insecticidal and acaricidal drugs.

[0019] The insecticidal and acaricidal composition of the present invention can be used for preventing and controlling pests, mites, underground pests or urban health pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, evergreen trees and the like; it can be particularly used for preventing and controlling piercing-sucking mouthpart pests on a variety of crops, such as planthoppers, whiteflies, aphids, scale insects, thrips, leafhoppers, rust mites, spider mites, as well as striped stem borers, corn borers, diamondback moths, armyworms, leaf miners, cotton bollworms, termites and other pests and urban health pests.

[0020] In particular, the insecticidal and acaricidal compositions of the present invention are particularly useful for controlling Hemiptera pests, a non-exhaustive list of which includes, but is not limited to, Acrosternum hilare, green stink bug, Blissus leucopterus, chinch bug, Calocoris norvegicus, Cimex hemipterus, tropical bed bug, Cimex lectularius, bed bug, Dagbertus fasciatus, Dichelops furcatus, Dysdercus suturellus, cotton bug, Edessa meditabunda, Eurygaster maura, cereal bug, Euschistus neotropicalis, heros), Euschistus servus, Brown stink bug, Helopeltis antonii, Helopeltis theivora, Tea horn stink bug, Lagynotomus spp., stink bugs, Leptocorisa oratorius, Leptocorisa varicornis, Lygus spp., Lygus, Lygus hesperus, Western grass stink bug, Maconellicoccus hirsutus, Neurocolpus longirostris, Nezara viridula, Southern green stink bug, Phytocoris spp., Lygus, California plant stink bug californicus), Phytocoris relativus, Piezodorus guildingi, Poecilocapsus lineatus, Four-lined stink bug, Psallus vaccinicola, Pseudacysta perseae, Scaptocoris castanea, and Triatoma spp., bloodsucking conenose bugs / kissing bugs.

[0021] In particular, the insecticidal and acaricidal compositions of the present invention are particularly useful for controlling homopteran pests (aphids, scale insects, whiteflies, planthoppers, leafhoppers), a non-exhaustive list of which includes, but is not limited to, pea aphids (Acrythosiphon pisum), pea aphids, Adelges spp., aphids, cabbage whitefly (Aleurodes proletella), cabbage whitefly, spiral whitefly (Aleurodicus disperses), soft-haired whitefly (Aleurothrixus floccosus), cotton whitefly, Aluacaspis spp., Amrasca bigutella bigutella, Aphrophora spp., leafhoppers, Aonidiella aurantii, Aphis spp., aphids of Aphis spp., cotton aphid (Aphis gossypii), cotton aphid, apple aphid (Aphis pomi), apple aphid, Aulacorthum solani, foxglove aphid, Bemisia spp., whitefly, Bemisia argentifolii, Bemisia tabaci, sweet potato whitefly, Brachycolus noxius, Russian aphid, Brachycorynella asparagi, asparagus aphid, Brevennia rehi, Brevicoryne brassicae, cabbage aphid, Ceroplastes spp. scale insects, Ceroplastes rubens, red wax scale insects, Chionaspis spp. scale insects, Chrysomphalus spp.), scale insects of the genus Coccus, Dysaphis plantaginea, pink apple aphid, leafhoppers of the genus Empoasca, woolly apple aphids Eriosoma lanigerum, woolly apple aphids, Icerya purchasi, woolly scale insects, Idioscopus nitidulus, mango leafhoppers, Laodelphax striatellus, brown planthoppers, Lepidosaphes, Macrosiphum.), potato aphid (Macrosiphum euphorbiae), potato aphid, wheat aphid (Macrosiphum granarium), English wheat aphid, rose aphid (Macrosiphum rosae), rose aphid, aster leafhopper (Macrosteles quadrilineatus), aster leafhopper, raspberry foamhopper (Mahanarva frimbiolata), wheat aphid (Metopolophium dirhodum), rose wheat aphid, long-horned bug (Mictis longicornis), peach aphid (Myzus persicae), green peach aphid, leafhoppers of the genus Nephotettix (Nephotettix spp.), black-tailed leafhopper (Nephotettix cinctipes), green leafhopper, green leafhopper, rice brown planthopper (Nilaparvata lugens), brown planthopper, bran shield scale (Parlatoria pergandii), bran scale, black scale scale (Parlatoria ziziphi), ebony scale, corn planthopper (Peregrinus maidis), corn flower-winged planthopper, Philaenus spp.'s scale, grape phylloxera (Phylloxera vitifoliae), grape phylloxera, spruce scale (Physokermes piceae), spruce round scale, planococcus spp., mealybugs, mealybugs of Pseudococcus spp., pineapple mealybug (Pseudococcus brevipes), pineapple mealybug, pear round scale (Quadraspidiotus perniciosus), St. Joseph's scale, Rhapalosiphum spp.), corn leaf aphids (Rhapalosiphummaida), corn aphids, grain aphids (Rhapalosiphum padi), grain aphids, scale insects of the genus Saissetia spp., olive black helmet scale insects (Saissetia oleae), black scale insects, Schizaphis graminum, green aphids, wheat long-tube aphids (Sitobion avenae), English wheat aphids, white-backed rice hoppers (Sogatella furcifera), white-backed planthoppers, aphids of the genus Therioaphis spp., scale insects of the genus Toumeyella spp., aphids of the genus Toxoptera spp., whiteflies (Trialeurodes spp.), greenhouse whitefly (Trialeurodes vaporariorum), greenhouse whitefly, green hemp whitefly (Trialeurodes abutiloneus), winged whitefly, scale insects of the genus Unaspis spp., Unaspis yanonensis, arrowhead scale insects, and Zulia entreriana.

[0022] In particular, the insecticidal and acaricidal compositions of the present invention are particularly useful for controlling thysanoptera pests (thrips), a non-exhaustive list of which includes, but is not limited to, Frankliniella fusca, tobacco thrips, Frankliniella occidentalis, western flower thrips, Frankliniella shultzei, Frankliniella williamsi, corn thrips, Heliothrips haemorrhaidalis, greenhouse thrips, Riphiphorothrips cruentatus, Scirtothrips spp., Scirtothrips citri, citrus thrips, Scirtothrips dorsalis, Scirtothrips dorsalis, Taeniothrips rhopalantennalis, and Thrips spp.

[0023] In particular, the insecticidal and acaricidal compositions of the present invention are particularly useful for controlling coleoptera (beetle) pests, a non-exhaustive list of which includes, but is not limited to: Acanthoscelides spp., weevils, Acanthoscelides obtectus, soybean weevils, Agrilus planipennis, ash borers, Agriotes spp., wireworms, Anoplophora glabripennis, Asian longhorn beetles, Anthonomus spp., weevils, Anthonomus grandis, boll weevils, Aphidius spp., Apion spp., weevils, Apogonia spp., grubs, Ataenius spretulus, turfgrass black beetle, beet cryptic beetle (Atomaria linearis), small beet beetle, melon beetle species (Aulacophora spp.), beet weevil (Bothynoderes punctiventris), beet root weevil, bean weevil species (Bruchus spp.), pea weevil (Bruchus pisorum), pea weevil, Cacoesia species, four-striped bean weevil (Callosobruchusmaculatus), southern cowpea weevil, yellow-spotted dew beetle (Carpophilus hemipteras), dried fruit beetle, beet tortoise beetle (Cassida vittata), Cerosterna species, Cerotoma species, leaf beetle, bean leaf beetle (Cerotomatrifurcata), bean leaf beetle, tortoise beetle species (Ceutorhynchus spp.), cabbage weevil (Ceutorhynchus assimilis), cabbage heart weevil, citrus weevil (Ceutorhynchus napi), cabbage weevil, Chaetocnema spp., Colaspis spp.), soil beetles, Conoderus scalaris, Conoderus stigmosus, Conotrachelus nenuphar, plum cone weevil, Cotinus nitidis, green flower beetle, Crioceris asparagi, asparagus leaf beetle, Cryptolestes ferrugineus, rusty flat flour beetle, Cryptolestes pusillus, flat flour beetle, Turkish flat flour beetle (Cryptolestesturcicus), Turkish flour beetle, Ctenicera spp., Curculio spp., Cyclocephala spp., Cylindrocpturus adspersus, sunflower stem weevil, Deporaus marginatus), mango leaf cutter weevil, Dermesteslardarius, ham weevil, Dermestes maculates, hidden beetle, Diabrotica spp. chrysolemid, Epilachna varivestis, Mexican bean beetle, Faustinus cubae, pale bark weevil, aggressive weevil, Hypera spp., Hypera postica, clover weevil, Hyperdoes spp., Argentine stem weevil, Hypothenemus hampei, coffee berry beetle, Ips spp., engraver beetle, Lasioderma serricorne), tobacco beetle, potato beetle (Leptinotarsa ​​decemlineata), Colorado potato beetle, Liogenys fuscus, Liogenys suturalis, Lissorhoptrus oryzophilus, rice water weevil, Lyctus spp., wood beetle, powder beetle, Maecolaspis joliveti, Megascelis spp., corn beetle (Melanotus communis), cauliflower beetle (Meligethes spp.), Meligethes aeneus, Flower beetles, Melolontha melolontha, Common European beetle, Oberea brevis, Oberea linearis, Oryctes rhinoceros, Date palm beetle, Oryzaephilus mercator, Market saw-toothed grain beetle, Oryzaephilus surinamensis, Saw-toothed grain beetle, Otiorhynchus spp., Oulema melanopus, Grain leaf beetle, Oulema oryzae, Pantomorus spp., Phyllophaga spp., May / June beetle, June beetle cuyabana), Phyllotreta spp., Phyllotretavittuta fabr., Yellow curvy flea beetle, Yellow straight flea beetle, Yellow narrow flea beetle, Yellow wide flea beetle, Leaf beetle, Phynchites spp., Popillia japonica, Japanese beetle, Prostephanus truncates, Rhizopertha dominica, Rhizopertha dominica, Rhizotrogus spp., European beetle, Rhynchophorus spp., Scolytus spp., Wood beetle), Shenophorus spp., Sitonalineatus, Pea leaf weevil, Sitophilus spp. spp.), cereal weevils, grain weevils (Sitophilusgranaries), grain worms, rice weevils (Sitophilus oryzae), rice weevils, small round skin beetles (Stegobiumpaniceum), stealing beetles, Tribolium spp., flour beetles, red flour beetles (Triboliumcastaneum), red flour beetles, mixed flour beetles (Triboliumconfusum), mixed flour beetles, spotted skin beetles (Trogodermavariabile), warehouse beetles and greedy carabids (Zabrustenebioides).

[0024] In particular, the insecticidal and acaricidal compositions of the present invention are particularly useful for controlling eggs, larvae, nymphs and adult mites of the order Acari, a non-exhaustive list of which includes, but is not limited to: spider mites and red mites of the family Tetranychidae, for example, Panonychus ulmi Koch, Tetranychus urticae Koch, Tetranychus mcdanieli McGregor; flat mites of the family Tenuipalpidae, for example, Brevipalpus lewisi McGregor; rust mites and bud mites of the family Eriophyidae. mite, as well as other leaf-feeding mites and mites of importance in human and animal health, namely, dust mites of the family Epidermoptidae, hair follicle mites of the family Demodicidae, grain mites of the family Glycyphagidae; ticks of the family Ixodidae, commonly known as hard ticks, e.g., deer tick (Ixodes scapularis Say), Australian paralysis tick (Ixodesholocyclus Neumann), American dog tick (Dermacentor variabilis Say), lone star tick (Amblyommaamericanum Linnaeus), and ticks of the family Argasidae, commonly known as soft ticks, e.g., relapsing fever tick (Ornithodoros turicata), common chicken tick (Argas radiatus); scabies of the families Psoroptidae, Pyemotidae, and Sarcoptidae. mite) and itch mite.

[0025] In particular, the insecticide and acaricide composition of the present invention can be used to control citrus panonychus mites, citrus rust mites, citrus Tetranychus spp., hawthorn Tetranychus spp., apple Panonychus mites, fruit moss mites, two-spotted Tetranychus spp., tea yellow mites, tea orange gall mites, tea short-palped mites, tea leaf gall mites, cinnabar Tetranychus spp., truncate Tetranychus spp., Turkestan Tetranychus spp., Dunhuang Tetranychus spp., wheat round red spider mites, wheat long-legged spiders, jujube ticks, Tetranychus spp. that harm jujube trees, Tetranychus spp. that harm jujube trees, pest mites on vegetables and flowers, citrus leaf miners, citrus scale insects, fruit tree Leaf miners, fruit borers, soybean borers, golden moths, tea geometrids, tea caterpillars, tea green leafhoppers, pear psyllids, rice planthoppers, greenhouse whiteflies, Bemisia tabaci, American leafminers, yellow-striped flea beetles, striped stem borers, yellow stem borers, corn borers, bean pod borers, melon borers, rice water weevils, cotton bollworms, pink bollworms, fruit aphids, soybean aphids, vegetable aphids, wheat aphids, cotton aphids, diamondback moths, armyworms, cabbage worms, tobacco budworms, longhorn beetles, mosquitoes, flies, cockroaches, termites and other pests, mites or urban health pests.

[0026] The insecticide and acaricide composition of the present invention can be applied in an effective dose to the pests / mites to be controlled and / or their habitats. Therefore, the insecticide and acaricide composition of the present invention provides a method for controlling or preventing plant pests.

[0027] The active ingredients in the insecticidal and acaricidal composition of the present invention can be applied to pests, plants and their habitats simultaneously (for example, as a preformulated mixture or tank mix) or in a suitable sequence.

[0028] The advantages of the present invention are as follows: the binary composition obtained by compounding component A with spirodiclofen, spirotetramat, spiromesifen, spiropidion and spirodiclofen in an appropriate ratio has a good synergistic effect, thereby improving the prevention effect, reducing the dosage and expanding the insecticide spectrum. Specifically: 1. The compounded two active components A and B have different action mechanisms and can delay the generation of drug resistance of pests and mites. 2. The control spectrum is expanded and can be used to control various pests and mites on various crops such as fruit trees, vegetables, ornamental plants, tea and cotton; 3. The amount of pesticide used is reduced, the amount of pesticide residues on crops is reduced, and the friendliness to the environment is enhanced. 4. The two active components A and B are compounded within an appropriate ratio range to improve the control effect of pests and mites such as cinnabarinus spider mite, whitefly, corn borer, cotton aphid and the like. DETAILED DESCRIPTION

[0029] The following examples are used to illustrate the present invention in detail, so as to facilitate further understanding of the present invention. The active component A is selected from compound I72 (prepared according to the description in the patent document CN112457288, with a content of 97%); the active component B and other raw materials are all commercially available products, and each component is measured by weight percentage.

[0030] Example 1: Indoor joint toxicity test of compound I72 and spiroclofen against Tetranychus cinnabarinus

[0031] Test target: Adult mites of Tetranychus cinnabarinus (boisduval), a sensitive strain reared indoors.

[0032] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0033] Test method: The potted seedling spray method was used to determine the activity against adult Tetranychus cinnabarinus. First, adult Tetranychus cinnabarinus mites of the same size were inoculated on the leaves of bean seedlings in the first pair of true leaves, and the base number was counted after the adult mites stabilized; then, according to the experimental design, the order from low dose to high dose was sprayed evenly, 1.5 mL per plant, 3 replicates per treatment, and a blank control was set.

[0034] The treated samples were placed in an observation room, where the temperature, humidity and light can be adjusted as needed. The number of dead and live mites was investigated after 72 hours. The corrected mortality rate was calculated using the Abbott formula, and statistical analysis was performed using the DPS data processing software to obtain the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect. The test results are shown in Table 1.

[0035] The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0036]

[0037] Where: ATI-actual toxicity index of the mixture; S-LC of the standard agent 50 ; LC of M-mixture 50 .

[0038] TTI=TIA×PA+TIB×PB

[0039] Where: TTI-theoretical toxicity index of mixture; TIA-toxicity index of A agent; percentage of PA-A agent in the mixture; TIB-toxicity index of B agent; percentage of PB-B agent in the mixture.

[0040]

[0041] Where: CTC-co-toxicity coefficient; ATI-actual toxicity index of mixture; TTI-theoretical toxicity index of mixture.

[0042] The co-toxicity coefficient (CTC) of the compound mixture: CTC ≥ 120 indicates a synergistic effect; 80 < CTC < 120 indicates an additive effect; CTC ≤ 80 indicates an antagonistic effect.

[0043] Table 1 Results of indoor joint toxicity determination of Compound I72 and spirodiclofen against Tetranychus cinnabarinus

[0044]

[0045] Example 2: Indoor joint toxicity determination test of Compound I-72 and spirotetramat against Thrips flavus

[0046] Test target: Nymphs of Thrips flavus Schrank.

[0047] Preparation of the liquid medicine: According to different test requirements, accurately weigh the test samples with an electronic analytical balance. The technical materials are dissolved in acetone and then diluted with 0.1% Tween 80 water into a series of liquid medicines with a certain concentration gradient according to the test design dose.

[0048] Test method: First, select eggplant seedlings carrying Thrips flavus, count the base number, and spray evenly in the order from low dose to high dose according to the test design, 2 mL per plant, with 3 replicates for each treatment, and set a blank control.

[0049] Place the treated test materials in the observation room, where the temperature, humidity, and light in the observation room can be adjusted as needed. After 48 hours, investigate the number of dead and live insects, calculate the corrected mortality rate using the Abbott formula, and perform statistical analysis with DPS data processing software to obtain the toxicity regression equations, LC 50 values and 95% confidence limits of each test single agent and each different ratio mixture, and then calculate the co-toxicity coefficient of each ratio using the Sun y-p method to evaluate the mixing effect (the same as in Example 1). The test results are shown in Table 2.

[0050] Table 2 Results of indoor joint toxicity determination of Compound I72 and spirotetramat against Thrips flavus

[0051]

[0052]

[0053] Example 3: Indoor joint toxicity determination test of Compound I-72 and spiromesifen against nymphs of Bemisia tabaci

[0054] Test target: Nymphs of Bemisia tabaci, a sensitive strain reared indoors.

[0055] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0056] Experimental method: First, select cotton leaves with neat and consistent whitefly nymphs and make leaf discs. Each leaf disc carries about 20 insects. Then, spray them evenly in the order from low dose to high dose according to the experimental design, 1.5 mL per leaf, and repeat each treatment 3 times. Set up a blank control.

[0057] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 96 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 3.

[0058] Table 3 Results of indoor combined toxicity test of compound I72 and spiromesifen on Bemisia tabaci nymphs

[0059]

[0060] Example 4: Indoor combined toxicity test of compound I-72 and methylphenidate on cotton aphids

[0061] Test target: cotton aphid (Aphis gossypii Glover) nymphs, a susceptible strain reared indoors.

[0062] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0063] Test method: First, select cotton leaves with neat and consistent cotton aphids, with each leaf carrying about 30-40 insects. Then, spray evenly from low dose to high dose according to the test design, 1.5 mL per leaf, repeat each treatment 3 times, and set up a blank control.

[0064] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 48 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 4.

[0065] Table 4 Results of indoor combined toxicity test on cotton aphids by mixing compound I72 and methylphenidate

[0066]

[0067] Example 5: Indoor combined toxicity test of compound I72 and spiroclofen against Tetranychus urticae

[0068] Test target: Adult two-spotted spider mite (Tetranychus urticae Koch), a sensitive strain raised indoors.

[0069] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0070] Test method: The pot seedling spray method was used to determine the activity against adult two-spotted spider mites. First, the two-spotted spider mites of the same size were inoculated on the leaves of bean seedlings in the first pair of true leaves. The base number of adult mites was counted after the adult mites stabilized; then, according to the experimental design, the order from low dose to high dose was sprayed evenly, 1.5 mL per plant, 3 replicates per treatment, and a blank control was set up.

[0071] The treated samples were placed in an observation room, where the temperature, humidity and light can be adjusted as needed. The number of dead and live mites was investigated after 72 hours. The corrected mortality rate was calculated using the Abbott formula, and statistical analysis was performed using the DPS data processing software to obtain the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 5.

[0072] Table 5 Results of indoor combined toxicity test of compound I72 and spiroclofenac against Tetranychus urticae

[0073]

[0074] Example 6: Indoor combined toxicity test of compound I-72 and spirotetramat against Acanthopanax spp.

[0075] Test target: Nymphs of Unaspis yanonensis Kuwana.

[0076] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0077] Test method: The activity against citrus scale nymphs was determined by the immersion method. First, the collected citrus leaves carrying scale nymphs were treated in the order from low dose to high dose according to the test design. After drying, the petioles were moisturized with absorbent cotton and then placed in a culture dish. Each treatment was repeated 3 times, and a blank control was set up.

[0078] The treated test materials were placed in an observation room, where the temperature, humidity and light intensity could be adjusted as needed. The number of dead and live insects was investigated after 120 hours. The corrected mortality rate was calculated using the Abbott formula, and statistical analysis was performed using the DPS data processing software to obtain the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 6.

[0079] Table 6 Results of indoor combined toxicity test of compound I-72 and spirotetramat against Acanthopanax spp.

[0080]

[0081]

[0082] Example 7: Indoor joint toxicity test of compound I-72 and methylphenidate against Plutella xylostella

[0083] Test target: 2nd instar larvae of diamondback moth (Plutella xylostella).

[0084] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0085] Experimental method: First, select cabbage leaves, and immerse them in the prepared solution for 10 seconds in the order from low dose to high dose according to the experimental design. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate neatly packed healthy test insects. Each treatment has 10 heads, and each treatment is repeated 3 times. A blank control is also set up.

[0086] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The co-toxicity coefficient of each ratio was calculated by Sun Yunpei's method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 7.

[0087] Table 7 Results of indoor combined toxicity test of compound I-72 and methylphenidate against Plutella xylostella

[0088]

[0089] The description of the above embodiments is only used to help understand the method of the present invention and its core idea. It should be pointed out that for ordinary technicians in this technical field, without departing from the principle of the present invention, the present invention can also be improved and modified in several ways, and these improvements and modifications also fall within the protection scope of the claims of the present invention. It can be seen from the above technical scheme that the insecticide and acaricide composition of the present invention has the advantages of improving drug efficacy, expanding insecticide spectrum, reducing the amount of pesticides used, reducing the amount of pesticide residues on crops, reducing environmental pollution, being safe for humans and animals, having good environmental compatibility, and pests are not easy to develop drug resistance. The composition of the present invention can be applied to the prevention and control of various pests and mites of various crops, and can be widely used in the field of agriculture.

Claims

1. A synergistic compound composition containing spirocyclic quaternary acid insecticides and acaricides, characterized in that: The composition comprises two active components A and B, wherein component A is selected from the piperic acid derivative compounds of the following structure, and component B is selected from B1 spirodiclofen, B2 spirotetramat, B3 spiromesifen, B5 spiropidion or B6 spirodiclofen; The weight ratio of the two active components A and B is 1:50 to 50:1; wherein the piperic acid derivative compound has the following structural formula:

2. The composition according to claim 1, characterized in that: The weight ratio of the two active components A and B is 1:10 to 10:

1.

3. The use of the composition according to claim 1, characterized in that: The composition is used for preparing insecticides and miticides for preventing and controlling Tetranychus pests, yellow thrips, whitefly, cotton aphid, scale insect and diamondback moth on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees and evergreen trees.

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