A synergistic compound composition containing pyrethroid insecticides and its application

Abstract

The present invention belongs to the field of pesticides and relates to a synergistic compound composition containing two active components. The composition contains two active components A and B, wherein component A is selected from the following structural compounds and component B is selected from pyrethroid insecticides; the weight ratio of the two active components of components A and B is 1:50 to 50:1; the composition of the present invention has an obvious synergistic effect and can be used to prevent and control a variety of pests and mites.

Description

Technical Field

[0001] The invention belongs to the field of pesticides and relates to a synergistic compound composition containing pyrethroid insecticides and application thereof. Background Art

[0002] As an important guarantee for stable and bumper crop yields, chemical pesticides play an irreplaceable role in pest control. Pyrethroid insecticides affect sodium ion channels in nerve membranes, causing neurotransmission disorders, which in turn lead to poisoning and death of pests. However, due to excessive input, the detoxification and metabolic capacity of pests is enhanced, the target sensitivity is reduced, and resistance is very likely to occur. The combination of different pesticides can not only delay the resistance of a single pesticide target and improve the activity of pesticides, but also reduce the amount of pesticides used and reduce the risk of environmental exposure, providing broad prospects for the sustainable application of pesticides.

[0003] Reasonable compounding or mixing of chemical agents has the positive characteristics of expanding the insecticide spectrum, improving the control effect, extending the application period, reducing the dosage, reducing the damage caused by the pesticide, reducing the residue, delaying the occurrence and development of the resistance and drug resistance of pests and mites, etc. Compounding or mixing of insecticides is one of the most effective ways to solve the above problems. The price of developing new insecticides is rising. In contrast, the development and research of high-efficiency, low-toxicity, and low-residue compounds and mixtures have low investment and short development cycle, and have attracted attention at home and abroad, and have increased their development efforts.

[0004] Chinese invention patent CN112457288 discloses a piperic acid derivative and its application, wherein compound I-72 has contact and stomach poison activity and good conduction activity, and can be used to control a variety of Lepidoptera, Hemiptera, Thysanoptera, Coleoptera and other pests. The compound has the following structural formula:

[0005]

[0006] The combination of different ingredients may produce some unexpected technical effects and is also an effective method for preventing and controlling resistant pests. However, there is no report on the combination of compound I-72 and pyrethroid insecticides. Summary of the invention

[0007] The purpose of the present invention is to provide a synergistic compound composition containing pyrethroid insecticides and application thereof.

[0008] To achieve the above purpose, the technical solution adopted by the present invention is:

[0009] A synergistic compound composition containing pyrethroid insecticides, the composition comprises two active components A and B, component A is selected from a piperic acid derivative compound (compound I-72) of the following structure, and component B is selected from a pyrethroid insecticide; the weight ratio of the two active components of components A and B is 1:50 to 50:1; wherein the structural formula of the piperic acid derivative compound is as follows:

[0010]

[0011] The pyrethroid insecticide is selected from B1 bifenthrin; B2 furethrin; B3 gamma-cyhalothrin; B4 barthrin; B5 metofluthrin; B6 fenpropathrin; B7 methotrexate; B8 terallethrin; B9 cyclethrin; B10 beta-cypermethrin permethrin); B11 lambda-cyhalothrin; B12 cypermethrin; B13 beta-cyfluthrin; B14 cyfluthrin; B15 tetramethrin; B16 permethrin; B17 etofenprox; B18 deltamethrin; B19 pyresmethrin; B20 Phenothrin; B21 cyphenothrin; B22 fenpirithrin; B23 resmethrin; B24 dimethrin; B25 butethrin; B26 transpermethrin; B27 theta-cypermethrin; B28 rich-dt-tetramethrin; B29 flucythrinate flucythrinate; B30 tan-fluvalinate; B31 proparthin; B32 acrinathrin; B33 silafluofen; B34 flumethrin; B35 pyrethrins; B36 empenthrin; B37 heptafluthrin; B38 meperfluthrin; B39 zeta-cypermethrin; B40 chlorempenthrin; B41 japothrins; B42 tefluthrin; B43 fenvalerate;B44 S-esfenvalerate; B45 prallethrin; B46 furamethrin; B47 imiprothrin; B48 kadethrin; B49 cyhalothrin; B50 flufenprox; B51 bioresmethrin; B52 biopermethrin; B53 bioallethrin; B54 S-bioallethrin; B55 cismethrin; B56 alpha-cypermethrin; B57 transfluthrin; B58 dimefluthrin; B59 tetramethylfluthrin; B60 tralomethrin; B61 fenfluthrin; B62 valerate; B63 pentmethrin; B64 allethrin; B65 bromomethrin omethrin); B66 brofluthrinate; B67 tralocythrin; B68 brofenvalerate; B69 cycloprothrin; B70 d-tetramethrin; B71 d-trans-tetramethrin; B72 d-trans-chloroprallethrin; B73 d-tetramethrin; B74 bio-allethrin; B75 chlorphenethrin; B76 d-allethrin; B77 d-trans-allethrin; B78 d-phenothrin; B79 d-phenothrin; B80 profluthrin; B81 momfluorothrin; B82 kappa-tefluthrin; B83 kappa-bifenthrin; B84 epsilon-momfluorothrin; B85 epsilon-metofluthrin; B86 α-cypermethrin; B87 cis-cypermethrin; B88 cyfluthrin; B89 dextro-trans-allethrin; B90 Es-bio-allethrin or B91 dextro-cypermethrin. ;

[0012] Preferably, in the composition, component A is selected from the above-mentioned piperic acid derivative compounds; component B is selected from B1 bifenthrin; B3 gamma-cyhalothrin; B5 metofluthrin; B6 fenpropathrin; B7 methotrexate; B10 beta-cypermethrin; B11 lambda-cyhalothrin; B12 cypermethrin; B13 beta-cyfluthrin; B14 cyfluthrin; B15 tetramethrin; B16 permethrin. thrin); B17 etofenprox; B18 deltamethrin; B20 phenothrin; B21 cyphenothrin; B23 resmethrin; B27 theta-cypermethrin; B28 rich-dt-tetramethrin; B32 acrinathrin; B33 silafluofen; B34 flumethrin; B35 pyrethrins; B36 empenthrin; B38 meperfluthrin; B39 zeta-cypermethrin; B40 chlorempenthrin; B43 fenvalerate; B44 esfenvalerate; B45 prallethrin; B47 imiprothrin; B53 bioallethrin; B54 S-bioallethrin; B55 cismethrin; B56 alpha-cypermethrin; B57 transfluthrin; B58 dimefluthrin; B59 tetramethylfluthrin; B64 allethrin; B70 d-tetramethrin;B71 d-trans-tetramethrin; B72 d-trans-chloroprallethrin; B86 α-cypermethrin; B87 cis-cypermethrin or B88 cyhalothrin;

[0013] The weight ratio of the two active components, component A and component B, is 1:20 to 20:1.

[0014] Further preferably, in the composition, component A is selected from the above-mentioned piperic acid derivative compounds; component B is selected from B1 bifenthrin; B11 lambda-cyhalothrin; B6 fenpropathrin; B10 beta-cypermethrin; B13 beta-cyfluthrin; B17 etofenprox; B43 fenvalerate; B18 deltamethrin or B35 pyrethrins;

[0015] The weight ratio of the two active components A and B is 1:10 to 10:1.

[0016] The application of the composition is used for preparing insecticides and miticides for preventing and controlling pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees and evergreen trees.

[0017] The composition is used for preventing or controlling pests of Lepidoptera, Hemiptera, Homoptera, Coleoptera, Diptera, Thysanoptera, Acarina, and their larvae and eggs.

[0018] The synergistic compound composition of the present invention has a significant synergistic effect in preventing and controlling pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, and evergreen trees. Indoor biological activity assay results show that mixing compound I-72 with pyrethroid insecticides can improve the prevention effect, reduce the dosage, and expand the insecticide spectrum. For example, when compound I-72 is mixed with bifenthrin, high-efficiency cyhalothrin, cypermethrin, high-efficiency cypermethrin, high-efficiency cyhalothrin, ethomethrin, cypermethrin, deltamethrin, etc. in a ratio range of 1:10 to 10:1, the activity against targets such as cinnabarin spider mite, whitefly, armyworm, corn borer, diamondback moth, brown planthopper, yellow flea beetle, peach aphid, etc. can be greatly improved. Mixing compound I-72 with pyrethroid insecticides can delay the target resistance of pyrethroid pesticides and reduce the high residue and non-target toxicity of pyrethroid pesticides. Therefore, the synergistic composite composition of the present invention can be used for the preparation of insecticide drugs.

[0019] The synergistic compound composition of the present invention can be used to control pests, mites and underground pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, evergreen trees and the like; it can be particularly used to control piercing-sucking mouthpart pests and lepidopteran pests, such as planthoppers, whiteflies, aphids, thrips, leafhoppers, stink bugs, as well as striped stem borers, corn borers, diamondback moths, armyworms, leaf miners, cotton bollworms, beet armyworms and the like on a variety of crops.

[0020] In particular, the synergistic composite composition of the present invention is particularly useful for controlling Hemiptera pests, a non-exhaustive list of which includes, but is not limited to, Acrosternum hilare, green stink bug, Blissus leucopterus, chinch bug, Calocoris norvegicus, Cimex hemipterus, tropical bed bug, Cimex lectularius, bed bug, Dagbertus fasciatus, Dichelops furcatus, Dysdercus suturellus, cotton bug, Edessa meditabunda, Eurygaster maura, cereal bug, Euschistus neotropicalis heros), Euschistus servus, Brown stink bug, Helopeltis antonii, Helopeltis theivora, Tea horn stink bug, Lagynotomus spp., stink bugs, Leptocorisa oratorius, Leptocorisa varicornis, Lygus spp., Lygus, Lygus hesperus, Western grass stink bug, Maconellicoccus hirsutus, Neurocolpus longirostris, Nezara viridula, Southern green stink bug, Phytocoris spp., Lygus, California plant stink bug californicus), Phytocoris relativus, Piezodorus guildingi, Poecilocapsus lineatus, Four-lined stink bug, Psallus vaccinicola, Pseudacysta perseae, Scaptocoris castanea, and Triatoma spp., bloodsucking conenose bugs / kissing bugs.

[0021] In particular, the synergistic composite composition of the present invention is particularly useful for controlling homopteran pests (aphids, scale insects, whiteflies, planthoppers, leafhoppers), a non-exhaustive list of which includes, but is not limited to, pea aphids (Acrythosiphon pisum), pea aphids, Adelges spp., aphids, cabbage whitefly (Aleurodes proletella), cabbage whitefly, spiral whitefly (Aleurodicus disperses), soft hair whitefly (Aleurothrixus floccosus), cotton whitefly, Aluacaspis spp., Amrasca bigutella bigutella, Aphrophora spp., leafhoppers, Aonidiella aurantii, Aphis spp., aphids of Aphis spp., cotton aphids (Aphis gossypii), cotton aphids, apple aphids (Aphis pomi), apple aphid, Aulacorthum solani, foxglove aphid, Bemisia spp., whitefly, Bemisia argentifolii, Bemisia tabaci, sweet potato whitefly, Brachycolus noxius, Russian aphid, Brachycorynella asparagi, asparagus aphid, Brevennia rehi, Brevicoryne brassicae, cabbage aphid, Ceroplastes spp. scale insects, Ceroplastes rubens, red wax scale insects, Chionaspis spp. scale insects, Chrysomphalus spp.), scale insects of the genus Coccus, Dysaphis plantaginea, pink apple aphid, leafhoppers of the genus Empoasca, woolly apple aphids Eriosoma lanigerum, woolly apple aphids, Icerya purchasi, woolly scale insects, Idioscopus nitidulus, mango leafhoppers, Laodelphax striatellus, brown planthoppers, Lepidosaphes, Macrosiphum.), potato aphid (Macrosiphum euphorbiae), potato aphid, wheat aphid (Macrosiphum granarium), English wheat aphid, rose aphid (Macrosiphum rosae), rose aphid, aster leafhopper (Macrosteles quadrilineatus), aster leafhopper, raspberry foamhopper (Mahanarva frimbiolata), wheat aphid (Metopolophium dirhodum), rose wheat aphid, long-horned bug (Mictis longicornis), peach aphid (Myzus persicae), green peach aphid, leafhoppers of the genus Nephotettix (Nephotettix spp.), black-tailed leafhopper (Nephotettix cinctipes), green leafhopper, green leafhopper, rice brown planthopper (Nilaparvata lugens), brown planthopper, bran shield scale (Parlatoria pergandii), bran scale, black scale scale (Parlatoria ziziphi), ebony scale, corn planthopper (Peregrinus maidis), corn flower-winged planthopper, Philaenus spp.'s scale, grape phylloxera (Phylloxera vitifoliae), grape phylloxera, spruce scale (Physokermes piceae), spruce round scale, planococcus spp., mealybugs, mealybugs of Pseudococcus spp., pineapple mealybug (Pseudococcus brevipes), pineapple mealybug, pear round scale (Quadraspidiotus perniciosus), St. Joseph's scale, Rhapalosiphum spp.), corn leaf aphids (Rhapalosiphummaida), corn aphids, grain aphids (Rhapalosiphum padi), grain aphids, scale insects of the genus Saissetia spp., olive black helmet scale insects (Saissetia oleae), black scale insects, Schizaphis graminum, green aphids, wheat long-tube aphids (Sitobion avenae), English wheat aphids, white-backed rice hoppers (Sogatella furcifera), white-backed planthoppers, aphids of the genus Therioaphis spp., scale insects of the genus Toumeyella spp., aphids of the genus Toxoptera spp., whiteflies (Trialeurodes spp.), greenhouse whitefly (Trialeurodes vaporariorum), greenhouse whitefly, green hemp whitefly (Trialeurodes abutiloneus), winged whitefly, scale insects of the genus Unaspis spp., Unaspis yanonensis, arrowhead scale insects, and Zulia entreriana.

[0022] In particular, the synergistic combination composition of the present invention is particularly useful for controlling Thysanoptera pests (thrips), a non-exhaustive list of which includes, but is not limited to, Frankliniella fusca, Tobacco thrips, Frankliniella occidentalis, Western flower thrips, Frankliniella shultzei, Frankliniella williamsi, Corn thrips, Heliothrips haemorrhaidalis, Greenhouse thrips, Riphiphorothrips cruentatus, Scirtothrips spp., Scirtothrips citri, Citrus thrips, Scirtothrips dorsalis, Scirtothrips dorsalis, Taeniothrips rhopalantennalis, and Thrips spp.

[0023] In particular, the synergistic composite composition of the present invention is particularly useful for controlling coleopteran (beetle) pests, a non-exhaustive list of which includes, but is not limited to: Acanthoscelides spp., weevils, Acanthoscelides obtectus, soybean weevils, Agrilus planipennis, ash borers, Agriotes spp., wireworms, Anoplophora glabripennis, Asian longhorn beetles, Anthonomus spp., weevils, Anthonomus grandis, boll weevils, Aphidius spp., Apion spp., weevils, Apogonia spp., grubs, Ataenius spretulus, turfgrass black beetle, beet cryptic beetle (Atomaria linearis), small beet beetle, melon beetle species (Aulacophora spp.), beet weevil (Bothynoderes punctiventris), beet root weevil, bean weevil species (Bruchus spp.), pea weevil (Bruchus pisorum), pea weevil, Cacoesia species, four-striped bean weevil (Callosobruchusmaculatus), southern cowpea weevil, yellow-spotted dew beetle (Carpophilus hemipteras), dried fruit beetle, beet tortoise beetle (Cassida vittata), Cerosterna species, Cerotoma species, leaf beetle, bean leaf beetle (Cerotomatrifurcata), bean leaf beetle, tortoise beetle species (Ceutorhynchus spp.), cabbage weevil (Ceutorhynchus assimilis), cabbage heart weevil, citrus weevil (Ceutorhynchus napi), cabbage weevil, Chaetocnema spp., Colaspis spp.), soil beetles, Conoderus scalaris, Conoderus stigmosus, Conotrachelus nenuphar, plum cone weevil, Cotinus nitidis, green flower beetle, Crioceris asparagi, asparagus leaf beetle, Cryptolestes ferrugineus, rusty flat flour beetle, Cryptolestes pusillus, flat flour beetle, Turkish flat flour beetle (Cryptolestesturcicus), Turkish flour beetle, Ctenicera spp., Curculio spp., Cyclocephala spp., Cylindrocpturus adspersus, sunflower stem weevil, Deporaus marginatus), mango leaf cutter weevil, Dermesteslardarius, ham weevil, Dermestes maculates, hidden beetle, Diabrotica spp. chrysolemid, Epilachna varivestis, Mexican bean beetle, Faustinus cubae, pale bark weevil, aggressive weevil, Hypera spp., Hypera postica, clover weevil, Hyperdoes spp., Argentine stem weevil, Hypothenemus hampei, coffee berry beetle, Ips spp., engraver beetle, Lasioderma serricorne), tobacco beetle, potato beetle (Leptinotarsa ​​decemlineata), Colorado potato beetle, Liogenys fuscus, Liogenys suturalis, Lissorhoptrus oryzophilus, rice water weevil, Lyctus spp., wood beetle, powder beetle, Maecolaspis joliveti, Megascelis spp., corn beetle (Melanotus communis), cauliflower beetle (Meligethes spp.), Meligethes aeneus, Flower beetles, Melolontha melolontha, Common European beetle, Oberea brevis, Oberea linearis, Oryctes rhinoceros, Date palm beetle, Oryzaephilus mercator, Market saw-toothed grain beetle, Oryzaephilus surinamensis, Saw-toothed grain beetle, Otiorhynchus spp., Oulema melanopus, Grain leaf beetle, Oulema oryzae, Pantomorus spp., Phyllophaga spp., May / June beetle, June beetle cuyabana), Phyllotreta spp., Phyllotretavittuta fabr., Yellow curvy flea beetle, Yellow straight flea beetle, Yellow narrow flea beetle, Yellow wide flea beetle, Leaf beetle, Phynchites spp., Popillia japonica, Japanese beetle, Prostephanus truncates, Rhizopertha dominica, Rhizopertha dominica, Rhizotrogus spp., European beetle, Rhynchophorus spp., Scolytus spp., Wood beetle), Shenophorus spp., Sitonalineatus, Pea leaf weevil, Sitophilus spp. spp.), cereal weevils, grain weevils (Sitophilusgranaries), grain worms, rice weevils (Sitophilus oryzae), rice weevils, small round skin beetles (Stegobiumpaniceum), stealing beetles, Tribolium spp., flour beetles, red flour beetles (Triboliumcastaneum), red flour beetles, mixed flour beetles (Triboliumconfusum), mixed flour beetles, spotted skin beetles (Trogodermavariabile), warehouse beetles and greedy carabids (Zabrustenebioides).

[0024] In particular, the synergistic combination composition of the present invention is particularly useful for controlling lepidopteran pests, a non-exhaustive list of which includes, but is not limited to: Pyralidae, such as Chilosuppressalis (Walker), Tryporyzaincertulas (Walker), Cnaphalocrocis medinalis Guenee, Hellullaundalis, Conogethes punctiferlis; Pieridae, such as Pieris rapae, Papilio xuthus, Pierisrapaecrucivora, Parnara guttata; Arctiidae, such as Hyphantria nymphae, cunea); Noctuidae, such as trichoplusiani, mamestra brassicae, spodoptera exigua, spodoptera litura, helicoverpaarmigera, Pseudaletia separate, Agrotis ipsilon; Tortricidae, such as adoxophyes orana fasciata, archipsfuscocureanus, homona magnanima; Plutellidae, such as plutella xylotella; Gelechiidae, such as pectinophoragossypiella, Pink bollworm, etc.

[0025] In particular, the synergistic compound composition of the present invention can be used to control citrus panonychus mite, citrus rust mite, citrus early spider mite, hawthorn spider mite, apple panonychus mite, two-spotted spider mite, tea yellow mite, tea orange gall mite, tea short-palped mite, tea gall mite, cinnabarinus spider mite, truncate spider mite, Turkestan spider mite, Dunhuang spider mite, wheat round red spider, wheat long-legged spider, jujube tick, citrus leaf miner, citrus scale insect, fruit tree leaf miner, Fruit borer, soybean borer, golden moth, tea geometrid, tea caterpillar, tea green leafhopper, pear psyllid, rice planthopper, greenhouse whitefly, American leafminer, yellow-striped flea beetle, striped stem borer, yellow stem borer, corn borer, bean pod borer, melon borer, rice water weevil, cotton bollworm, pink bollworm, fruit aphid, soybean aphid, vegetable aphid, wheat aphid, cotton aphid, diamondback moth, armyworm, cabbage worm, tobacco budworm, longhorn beetle and other pests and mites.

[0026] The synergistic compound composition of the present invention can be applied at an effective dose to the pests / mites to be controlled and / or their habitats. Therefore, the synergistic compound composition of the present invention provides a method for controlling or preventing plant pests.

[0027] The active ingredients in the synergistic compound composition of the present invention can be applied to pests, plants and their habitats simultaneously (for example, as a preformulated mixture or tank mix) or in a suitable sequence.

[0028] Advantages of the present invention: The binary composition obtained by compounding compound I-72 and pyrethroid insecticide in a suitable ratio has a good synergistic effect, thereby improving the prevention effect, reducing the dosage, and expanding the insecticide spectrum. Specifically, 1. The compounded two active components A and B have different mechanisms of action, which can delay the development of drug resistance in pests and mites. 2. The insecticide spectrum is expanded, and it can be used to prevent and control various pests and mites on various crops such as fruit trees, vegetables, ornamental plants, tea, and cotton; 3. The amount of pesticide used is reduced, the amount of pesticide residues on crops is reduced, and the friendliness to the environment is enhanced. 4. The two active components A and B are compounded within a suitable ratio range to improve the control effect of pests and mites such as cinnabar spider mites, whiteflies, armyworms, corn borers, diamondback moths, brown planthoppers, yellow flea beetles, and peach aphids. DETAILED DESCRIPTION

[0029] The following examples are used to explain the present invention in detail, so as to facilitate further understanding of the present invention. The active component A is selected from compound I-72 (prepared according to the description in the patent document CN112457288, with a content of 97%); the active component B and other raw materials are all commercially available products, and each component is measured by weight percentage.

[0030] Example 1: Indoor synergistic screening test of compound I-72 mixed with different pyrethroid insecticides against plum moth Yponomeuta evonymallus (Linnaeus) Test target: 3rd instar larvae of plum moth Yponomeuta evonymallus (Linnaeus).

[0031] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0032] Experimental method: First, select Sedum leaves, and immerse them in the prepared solution for 10 seconds in the order from low dose to high dose according to the experimental design. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate neatly arranged healthy test insects. Each treatment has 10 heads, and each treatment is repeated 3 times. A blank control (CK) is also set up.

[0033] The treated test materials were placed in an observation room where the temperature, humidity and light intensity could be adjusted as needed. The number of dead and live insects was investigated after 72 hours and the corrected mortality rate was calculated using the Abbott formula.

[0034] The Bliss method was used to evaluate the synergistic effect of mixed pesticides. Based on the concept of independent combined effects proposed by Bliss, he believed that the theoretical mortality rate P of mixed pesticides can be calculated by the following formula:

[0035] P=Pm+Pn(1-Pm)

[0036] Pm is the measured mortality rate (%) of the target when the concentration of the first active component is m; Pn is the measured mortality rate (%) of the target when the concentration of the second active component is n.

[0037] If the actual mortality rate of the target after the two active components are mixed at a certain concentration is greater than the theoretical mortality rate P, it is determined that the two active components have a synergistic effect when mixed at the set concentration, otherwise it is an antagonistic effect. The test results are shown in Table 1.

[0038] Table 1 Results of indoor synergistic screening of compound I-72 mixed with different pyrethroid insecticides against clump moth

[0039]

[0040]

[0041]

[0042] Example 2: Indoor synergistic screening test of compound I-72 mixed with different pyrethroid insecticides against broad bean aphids Test target: broad bean aphid (Aphis craccivora) nymphs, a sensitive strain reared indoors.

[0043] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0044] Experimental method: First, select broad bean seedlings with neat and consistent broad bean aphids, with an insect load of about 30-40 heads per plant, and then spray evenly in order from low dose to high dose according to the experimental design, 1.5mL per leaf, and repeat each treatment 3 times, with a blank control (CK) set up.

[0045] The treated test materials were placed in an observation room where the temperature, humidity and light intensity could be adjusted as needed. The number of dead and live insects was investigated after 48 hours and the corrected mortality rate was calculated using the Abbott formula.

[0046] The synergistic effect of the mixture was evaluated by the Bliss method, which was the same as in Example 1. The test results are shown in Table 2.

[0047] Table 2 Results of indoor synergistic screening of compound I-72 mixed with different pyrethroid insecticides against broad bean aphids

[0048]

[0049]

[0050] Example 3: Indoor joint toxicity test of compound I-72 and cypermethrin against Tetranychus cinnabarinus

[0051] Test target: Adult mites of Tetranychus cinnabarinus (boisduval), a sensitive strain reared indoors.

[0052] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0053] Test method: The potted seedling spray method was used to determine the activity against adult Tetranychus cinnabarinus. First, adult Tetranychus cinnabarinus mites of the same size were inoculated on the leaves of bean seedlings in the first pair of true leaves, and the base number was counted after the adult mites stabilized; then, according to the experimental design, the order from low dose to high dose was sprayed evenly, 1.5 mL per plant, 3 replicates per treatment, and a blank control was set.

[0054] The treated samples were placed in an observation room, where the temperature, humidity and light can be adjusted as needed. The number of dead and live mites was investigated after 72 hours. The corrected mortality rate was calculated using the Abbott formula, and statistical analysis was performed using the DPS data processing software to obtain the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture.50 The values and 95% confidence limits were obtained, and the co-toxicity coefficient of each formulation was calculated using the Sun Y-p method to evaluate the mixing effect. The test results are shown in Table 3.

[0055] The co-toxicity coefficient (CTC value) of the mixture was calculated according to the following formula:

[0056]

[0057] Where: ATI - the actual toxicity index of the mixture; S - the LC of the standard agent 50 ; M - the LC of the mixture 50 .

[0058] TTI = TIA × PA + TIB × PB

[0059] Where: TTI - the theoretical toxicity index of the mixture; TIA - the toxicity index of agent A; PA - the percentage content of agent A in the mixture; TIB - the toxicity index of agent B; PB - the percentage content of agent B in the mixture.

[0060]

[0061] Where: CTC - co-toxicity coefficient; ATI - the actual toxicity index of the mixture; TTI - the theoretical toxicity index of the mixture.

[0062] The co-toxicity coefficient (CTC) of the compound mixture: CTC ≥ 120 indicates a synergistic effect; 80 < CTC < 120 indicates an additive effect; CTC ≤ 80 indicates an antagonistic effect.

[0063] Table 3 Results of indoor combined toxicity determination of compound I-72 and fenpropathrin against Tetranychus cinnabarinus

[0064]

[0065] Example 4: Indoor combined toxicity determination test of compound I-72 and bifenthrin against Bemisia tabaci

[0066] Test target: Nymphs of Bemisia tabaci, a sensitive strain reared indoors.

[0067] Preparation of the liquid medicine: According to different test requirements, the test samples were accurately weighed separately using an electronic analytical balance. The technical materials were dissolved in acetone and then diluted with 0.1% Tween 80 aqueous solution into a series of liquid medicines with a certain concentration gradient according to the test design dose.

[0068] Test method: First, select cotton leaves with neat and consistent nymphs of Bemisia tabaci to make leaf discs, with about 20 insects per leaf disc. Then, spray evenly in the order of low dose to high dose according to the test design, 1.5 mL per leaf, and each treatment was replicated 3 times. A blank control was also set up. It should be noted that there is an error in the original text in item . It should be "80 < CTC < 120" instead of "120 < CTC < 120" for the description of the additive effect. The translation has been corrected accordingly.

[0069] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 96 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 3). The test results are shown in Table 4.

[0070] Table 4 Results of indoor combined toxicity test of compound I-72 and bifenthrin against Bemisia tabaci

[0071]

[0072]

[0073] Example 5: Indoor combined toxicity test of compound I-72 and high-efficiency cyfluthrin against armyworms

[0074] Test target: 2nd instar larvae of armyworm (Mythimna seperata).

[0075] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0076] Experimental method: First, select corn leaves and immerse them in the prepared solution for 10 seconds in the order from low dose to high dose according to the experimental design. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate neatly packed healthy test insects. Each treatment has 10 heads and each treatment is repeated 3 times. A blank control is also set up.

[0077] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 3). The test results are shown in Table 5.

[0078] Table 5 Results of indoor combined toxicity test of compound I-72 and high-efficiency cyfluthrin against armyworms

[0079]

[0080] Example 6: Indoor joint toxicity test of compound I-72 and high-efficiency cyhalothrin against corn borer

[0081] Test target: 3rd instar larvae of corn borer (Ostrinia furnacnlis Guenee).

[0082] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0083] Experimental method: First, select tender corn stalks and immerse them in the prepared solution for 10 seconds in the order from low dose to high dose according to the experimental design. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate neatly packed healthy test insects. Each treatment has 10 heads and each treatment is repeated 3 times. A blank control is also set up.

[0084] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 3). The test results are shown in Table 6.

[0085] Table 6 Results of indoor combined toxicity test of compound I-72 and high-efficiency cyhalothrin against corn borer

[0086]

[0087] Example 7: Indoor joint toxicity test of compound I-72 and highly effective cypermethrin against Plutella xylostella

[0088] Test target: 2nd instar larvae of diamondback moth (Plutella xylostella).

[0089] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0090] Experimental method: First, select cabbage leaves, and immerse them in the prepared solution for 10 seconds in the order from low dose to high dose according to the experimental design. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate neatly packed healthy test insects. Each treatment has 10 heads, and each treatment is repeated 3 times. A blank control is also set up.

[0091] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 3). The test results are shown in Table 7.

[0092] Table 7 Results of indoor combined toxicity test of compound I-72 and highly effective cypermethrin against Plutella xylostella

[0093]

[0094]

[0095] Example 8: Indoor joint toxicity test of compound I-72 and ethoxyfenpropathrin against rice brown planthopper

[0096] Test target: Rice brown planthopper [Nilaparvata lugens (Stal)] nymphs, a susceptible strain reared indoors.

[0097] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0098] Test method: The pot seedling spray method was used to determine the activity against rice brown planthopper nymphs. First, rice seedlings were planted in a culture pot and sprayed evenly in the order from low dose to high dose according to the experimental design, 2 mL per plant, and then rice brown planthopper nymphs of the same size were inoculated on the rice seedlings. Each treatment was repeated 3 times, and a blank control was set up.

[0099] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 3). The test results are shown in Table 8.

[0100] Table 8 Results of indoor combined toxicity test of compound I-72 and ethoxyfenpropathrin against rice brown planthopper

[0101]

[0102] Example 9: Indoor joint toxicity test of compound I-72 and deltamethrin against yellow striped flea beetle

[0103] Test target: Adult yellow-striped flea beetle (Phyllotreta vittuta Fabr.).

[0104] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0105] Experimental method: First, select neat and consistent potted cabbage seedlings, and then spray them evenly in order from low dose to high dose according to the experimental design. After drying in the shade, transfer the treated cabbage seedlings to the adult flea beetles and cover them with glass covers, about 30-40 heads per plant. Each treatment is repeated 3 times, and a blank control is set up.

[0106] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 3). The test results are shown in Table 9.

[0107] Table 9 Results of indoor combined toxicity test of compound I-72 and deltamethrin against yellow striped flea beetle

[0108]

[0109] Example 10: Indoor combined toxicity test of compound I-72 and fenvalerate against green peach aphid

[0110] Test target: Green peach aphid [Myzus persicae (Sulzer)] nymphs, a susceptible strain reared indoors.

[0111] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0112] Test method: First, select cabbage leaves with neat and uniform peach aphids, with each leaf carrying about 30-40 insects. Then, spray evenly from low dose to high dose according to the test design, 1.5 mL per leaf, repeat each treatment 3 times, and set up a blank control.

[0113] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 48 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 3). The test results are shown in Table 10.

[0114] Table 10 Results of indoor combined toxicity test of compound I-72 and fenvalerate against green peach aphid

[0115]

[0116]

[0117] Example 11: Indoor joint toxicity test of compound I-72 and pyrethrin against radish aphid

[0118] Test target: Radish aphid [Lipaphis erysimi (Kaltenbach)].

[0119] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0120] Test method: First, select the cabbage leaves with neat and uniform radish aphids, with each leaf carrying about 30-40 insects. Then, spray evenly from low dose to high dose according to the test design, 1.5 mL per leaf, repeat each treatment 3 times, and set up a blank control.

[0121] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 48 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 3). The test results are shown in Table 11.

[0122] Table 11 Results of indoor combined toxicity test on compound I-72 and pyrethrin against radish aphid

[0123]

[0124] The description of the above embodiments is only used to help understand the method of the present invention and its core idea. It should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, the present invention can also be improved and modified in several ways, and these improvements and modifications also fall within the scope of protection of the claims of the present invention. It can be seen from the above technical scheme that the synergistic compound composition of the present invention has the advantages of improving drug efficacy, expanding insecticide spectrum, reducing pesticide dosage, reducing pesticide residues on crops, reducing environmental pollution, being safe for humans and animals, having good environmental compatibility, and pests are not easy to develop drug resistance. The composition of the present invention can be applied to the prevention and treatment of various pests and mites of various crops, and can be widely used in the field of agriculture.

Claims

1. A synergistic compound composition containing pyrethroid insecticides, characterized in that The composition comprises two active components, A and B, wherein component A is selected from the piperic acid derivative compounds of the following structure, and component B is selected from pyrethroid insecticides; the weight ratio of the two active components, component A and component B, is 1:50 to 50:1; wherein the structural formula of the piperic acid derivative compound is as follows: The pyrethroid insecticide is selected from B1 bifenthrin; B3 gamma-cyhalothrin; B6 fenpropathrin; B10 beta-cypermethrin; B11 lambda-cyhalothrin; B12 cypermethrin; B13 beta-cyfluthrin; B14 cyfluthrin; B16 permethrin; B17 etofenprox; B18 deltamethrin; B27 theta-cypermethrin; B35 pyrethrins; B39 zeta-cypermethrin; B43 fenvalerate; B44 esfenvalerate; B56 alpha-cypermethrin.

2. The composition according to claim 1, characterized in that: Component A in the composition is selected from the above-mentioned piperic acid derivative compounds; the weight ratio of the two active components, component A and component B, is 1:20 to 20:

1.

3. The composition according to claim 2, characterized in that: Component A in the composition is selected from the above-mentioned piperic acid derivative compounds; component B is selected from B1 bifenthrin; B11 lambda-cyhalothrin; B6 fenpropathrin; B10 beta-cypermethrin; B13 beta-cyfluthrin; B17 etofenprox; B43 fenvalerate; B18 deltamethrin or B35 pyrethrins; The weight ratio of the two active components A and B is 1:10 to 10:

1.

4. The use of the composition according to claim 1, characterized in that: The composition is used for preparing insecticides and miticides for controlling pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, and evergreen trees; The pests and mites are one or more of the group consisting of the thick plum nest moth, broad bean aphid, peach aphid, radish aphid, cinnabarinus spider mite, Bemisia tabaci, Noctuidae, Pyralidae, Plutella xylostella, rice brown planthopper, and yellow-striped flea beetle.

5. The use of the composition according to claim 1, characterized in that: The composition is used for preventing or controlling plum nest moth, broad bean aphid, peach aphid, radish aphid, cinnabarinus spider mite, whitefly, Noctuidae, Pyralidae, Plutella xylostella, rice brown planthopper, yellow striped flea beetle pests and their larvae and eggs.

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