A traditional Chinese medicine composition for invigorating qi and activating blood circulation and its application in preparing a drug for treating cardiovascular aging

Abstract

The present invention relates to a Chinese medicine composition for invigorating qi and promoting blood circulation and its application in preparing a drug for treating cardiovascular aging. The composition comprises 13 kinds of Chinese medicinal materials: epimedium and / or epimedium extract, schisandra and / or schisandra extract, gynostemma pentaphyllum and / or gynostemma pentaphyllum extract, psoralea corylifolia and / or psoralea corylifolia extract, acanthopanax and / or acanthopanax extract, ginseng and / or ginseng extract, licorice and / or licorice extract, osmanthus fragrans and / or osmanthus fragrans extract, immature bitter orange and / or immature bitter orange extract, tangerine peel and / or tangerine peel extract, angelica and / or angelica extract, white peony root and / or white peony root extract, red peony root and / or red peony root extract. The Chinese medicine composition for invigorating qi and promoting blood circulation of the present invention is obtained by extracting and matching different active ingredients of 13 kinds of Chinese medicinal materials to obtain the best formula, which can significantly improve the antioxidant activity of the body, promote macrophage proliferation, slow down the aging speed of the body, and maintain the vitality of the body.

Description

Technical Field

[0001] The invention belongs to the field of pharmaceutical technology, and specifically relates to a traditional Chinese medicine composition for invigorating qi and activating blood circulation and an application thereof in preparing a medicine for treating cardiovascular aging. Background Art

[0002] With the rapid increase of the aging population, aging-related diseases such as cardiovascular disease, diabetes, and Alzheimer's disease are increasingly threatening people's lives and health. Cardiovascular aging is an important hub connecting aging and aging-related diseases. With aging, the function of the aging cardiovascular system changes significantly, leading to the occurrence of cardiovascular diseases such as atherosclerosis, hypertension, and myocardial infarction. The health of the cardiovascular system is crucial to the health of the body, so intervening in cardiovascular aging is of great significance in delaying the human aging process and preventing aging-related diseases. The eight characteristics of cardiovascular aging include loss of macrophage function, loss of protein homeostasis, genomic instability, epigenetic changes, mitochondrial dysfunction, cellular senescence, neurohormonal signaling disorders, and inflammation (Abdellatif, M., Rainer, PP, Sedej, S. et al. Hallmarks of cardiovascularageing. Nat Rev Cardiol20,754-777(2023).), the drugs for the treatment of cardiovascular aging are currently mainly Western medicine, Chinese and Western medicine, and according to Western modern medical theories and modern scientific methods, there are three major categories of drugs, including organic chemical components, inorganic chemical components and biomolecular drugs. The ingredients, chemical structures and dosages of the drugs are very precise, including statins, beta-blockers, angiotensin converting enzyme inhibitors, etc., which can be used to treat cardiovascular diseases. However, long-term use in the treatment of chronic diseases such as cardiovascular aging will bring serious toxic side effects. Once the body develops drug resistance, the therapeutic effect of the drug will be reduced, and the therapeutic effect of Western medicine will be significantly reduced. In actual clinical treatment, the drug dosage is often increased to make up for the loss of efficacy, resulting in greater toxic side effects, thus falling into a vicious circle of drug treatment. Therefore, at present, the treatment direction of chronic diseases such as cardiovascular aging is gradually turning to Chinese medicine and the combination of Chinese and Western medicine.

[0003] At present, the practice of traditional Chinese medicine treatment for cardiovascular diseases shows that traditional Chinese medicine raw materials or traditional Chinese medicine compound preparations such as Danshen, Ginkgo extract, Shengmai Yin, Xinning tablets, etc. have good therapeutic effects. In addition, CN113318193A discloses a traditional Chinese medicine composition with the effect of delaying myocardial cell aging. The composition uses ginseng, Panax notoginseng, Chuanxiong and Dendrobium as active ingredients to regulate telomeres and telomerase, and delay myocardial cell aging; CN110051731A discloses a traditional Chinese medicine composition for delaying aging, which is prepared by compounding 12-30 parts of Epimedium and 8-24 parts of Ligustrum lucidum, and is developed for the aging pathogenesis caused by "insufficient kidney essence and imbalance of yin and yang"; although the traditional Chinese medicine compound has achieved great success in treatment and shows lower toxic side effects during long-term treatment. However, these drugs also have the common defects of traditional Chinese medicine, namely, slow onset of efficacy and unclear therapeutic mechanism. At the same time, due to the wide variety of Chinese medicinal materials, the therapeutic mechanism and dosage are difficult to clarify. Therefore, the search and development of traditional Chinese medicine and traditional Chinese medicine compound preparations remains a difficult problem in the clinical treatment of traditional Chinese medicine. Summary of the invention

[0004] In view of the above-mentioned existing technical problems, the present invention provides a traditional Chinese medicine composition for invigorating qi and promoting blood circulation and its application in the preparation of drugs for treating cardiovascular aging. The traditional Chinese medicine composition for invigorating qi and promoting blood circulation is obtained by processing 13 kinds of Chinese medicinal materials and scientifically combining them. The traditional Chinese medicine composition for invigorating qi and promoting blood circulation can significantly improve the body's anti-chemical activity and macrophage activity, promote macrophage proliferation, slow down the body's aging rate, and maintain the body's vitality.

[0005] In a first aspect, the present invention provides a traditional Chinese medicine composition for invigorating qi and activating blood circulation, wherein the Chinese medicinal materials of the traditional Chinese medicine composition include epimedium and / or epimedium extract, schisandra chinensis and / or schisandra chinensis extract, gynostemma pentaphyllum and / or gynostemma pentaphyllum extract, psoralea corylifolia and / or psoralea corylifolia extract, Acanthopanax senticosus and / or Acanthopanax senticosus extract, ginseng and / or ginseng extract, licorice and / or licorice extract, coix lacryma-jobi and / or coix lacryma-jobi extract, Citrus aurantium and / or Citrus aurantium extract, tangerine peel and / or tangerine peel extract, angelica sinensis and / or angelica sinensis extract, white peony root and / or white peony root extract, red peony root and / or red peony root extract.

[0006] Furthermore, in parts by mass, the Chinese medicinal materials of the Chinese medicine composition include 0.1-10 parts by mass of epimedium and / or epimedium extract, 0.5-10 parts by mass of schisandra and / or schisandra extract, 3-15 parts by mass of gynostemma pentaphyllum and / or gynostemma pentaphyllum extract, 1-5 parts by mass of psoralea corylifolia and / or psoralea corylifolia extract, 0.5-20 parts by mass of Acanthopanax senticosus and / or Acanthopanax senticosus extract, 20-55 parts by mass of ginseng and / or ginseng extract, 5-15 parts by mass of licorice and / or licorice extract, 1.5-15 parts by mass of osmanthus fragrans and / or osmanthus fragrans extract, 0.5-8 parts by mass of immature orange and / or immature orange extract, 3-8 parts by mass of tangerine peel and / or tangerine peel extract, 15-40 parts by mass of angelica and / or angelica extract, 1-20 parts by mass of white peony root and / or white peony root extract, and 0.1-5 parts by mass of red peony root and / or red peony root extract.

[0007] Furthermore, the present invention provides a preferred formula of a Chinese medicine composition for invigorating qi and promoting blood circulation, wherein the Chinese medicinal materials of the Chinese medicine composition include epimedium, schisandra chinensis, gynostemma pentaphyllum, psoralea corylifolia, Acanthopanax senticosus, ginseng, licorice, osmanthus fragrans, immature bitter orange, tangerine peel, angelica sinensis, white peony root and / or red peony root.

[0008] Furthermore, measured by weight, the Chinese medicinal materials of the Chinese medicine composition include 0.1-10 parts by weight of epimedium, 0.5-10 parts by weight of Schisandra chinensis, 3-15 parts by weight of Gynostemma pentaphyllum, 1-5 parts by weight of Psoralea corylifolia, 0.5-20 parts by weight of Acanthopanax senticosus, 20-55 parts by weight of Ginseng, 5-15 parts by weight of Licorice, 1.5-15 parts by weight of Glehnia littoralis, 0.5-8 parts by weight of Citrus aurantium, 3-8 parts by weight of Citrus reticulatae, 15-40 parts by weight of Angelica sinensis, 1-20 parts by weight of White Peony Root and / or 0.1-5 parts by weight of Red Peony Root.

[0009] Furthermore, the present invention provides another preferred formula of a Chinese medicine composition for invigorating qi and activating blood circulation, the Chinese medicinal materials of the Chinese medicine composition comprising epimedium and / or epimedium extract, schisandra chinensis and / or schisandra chinensis extract, gynostemma pentaphyllum and / or gynostemma pentaphyllum extract, psoralea corylifolia and / or psoralea corylifolia extract, Acanthopanax senticosus and / or Acanthopanax senticosus extract, ginseng, licorice, osmanthus fragrans and / or osmanthus fragrans extract, immature bitter orange and / or immature bitter orange extract, tangerine peel, angelica sinensis, white peony root and / or white peony root extract, red peony root and / or red peony root extract.

[0010] More preferably, the Chinese medicinal materials raw materials of the Chinese medicine composition include, by weight, 0.1-10 parts of epimedium extract, 0.5-10 parts of Schisandra extract, 3-15 parts of Gynostemma extract, 1-5 parts of Psoralea corylifolia extract, 0.5-20 parts of Acanthopanax senticosus extract, 20-55 parts of ginseng, 5-15 parts of Licorice, 1.5-15 parts of Glechoma longifolia extract, 0.5-8 parts of Citrus aurantium extract, 3-8 parts of Citrus reticulatae, 15-40 parts of Angelica sinensis, 1-20 parts of White Peony Root Extract, and 0.1-5 parts of Red Peony Root Extract.

[0011] Furthermore, the present invention also provides another preferred formula of a traditional Chinese medicine composition for invigorating qi and activating blood circulation, the traditional Chinese medicine raw materials of the traditional Chinese medicine composition comprising epimedium extract I, epimedium extract II, schisandra extract I, schisandra extract II, gynostemma extract I, gynostemma extract II, psoralea corylifolia extract, Acanthopanax senticosus extract I, Acanthopanax senticosus extract II, Acanthopanax senticosus extract III, ginseng, licorice, radix sibiricum extract I, radix sibiricum extract II, radix sibiricum extract III, immature bitter orange extract I, immature bitter orange extract II, immature bitter orange extract III, tangerine peel, angelica sinensis, white peony extract I, white peony extract II, white peony extract III, red peony extract I, red peony extract II, red peony extract III.

[0012] Further, in parts by mass, the Chinese medicinal material raw materials of the Chinese medicine composition include 0.1-0.5 parts by mass of epimedium extract I, 5-10 parts by mass of epimedium extract II, 1-5 parts by mass of schisandra extract I, 0.5-2 parts by mass of schisandra extract II, 5-10 parts by mass of gynostemma extract I, 3-8 parts by mass of gynostemma extract II, 1-2 parts by mass of psoralea corylifolia extract, 5-10 parts by mass of Acanthopanax senticosus extract I, 5-10 parts by mass of Acanthopanax senticosus extract II, 0.5-2.5 parts by mass of Acanthopanax senticosus extract III, 20-55 parts by mass of ginseng, 5-15 parts by mass of liquorice, 2-5 parts by mass of scutellaria baicalensis extract I, 2-5 parts by mass of scutellaria baicalensis extract II, 1.5-2.5 parts by mass of scutellaria baicalensis extract III, 1-3 parts by mass of Citrus aurantium extract I, 0.5-2 parts by mass of Citrus aurantium extract II, and 0.5-2 parts by mass of Citrus aurantium extract III. 2 to 5 parts by mass, tangerine peel 3 to 8 parts by mass, angelica 15 to 40 parts by mass, white peony extract I 1 to 5 parts by mass, white peony extract II 5 to 15 parts by mass, white peony extract III 1 to 3 parts by mass, red peony extract I 0.1 to 0.5 parts by mass, red peony extract II 0.1 to 2 parts by mass, and red peony extract III 0.5 to 2 parts by mass.

[0013] Furthermore, the traditional Chinese medicine composition for invigorating qi and promoting blood circulation of the present invention may also include pharmaceutically acceptable excipients;

[0014] The excipients include thickeners, fillers, binders, wetting agents, disintegrants, lubricants, etc., but are not limited to the types of pharmaceutical excipients listed above. Other types of pharmaceutical excipients not listed are also applicable;

[0015] Furthermore, the excipients include gelatin, sucrose, mannitol, sorbitol, mannitol, sorbitol, talc, silica gel, magnesium stearate, starch, dextrin, lactose, microcrystalline cellulose, polyethylene glycol, mucilage, povidone, cellulose derivatives, etc., but are not limited to the pharmaceutical excipients listed above. Other excipients not listed within the above-mentioned pharmaceutical excipient types are also applicable.

[0016] In a second aspect, the present invention provides a method for preparing the traditional Chinese medicine composition for invigorating qi and promoting blood circulation, comprising the following steps:

[0017] The Chinese medicinal materials including epimedium and / or epimedium extract, schisandra and / or schisandra extract, gynostemma pentaphyllum and / or gynostemma pentaphyllum extract, psoralea corylifolia and / or psoralea corylifolia extract, Acanthopanax senticosus and / or Acanthopanax senticosus extract, ginseng and / or ginseng extract, licorice and / or licorice extract, osmanthus fragrans and / or osmanthus fragrans extract, immature bitter orange and / or immature bitter orange extract, tangerine peel and / or tangerine peel extract, angelica and / or angelica extract, white peony root and / or white peony root extract, red peony root and / or red peony root extract are crushed, sieved, and made into powder of 20-300 mesh, and fully mixed;

[0018] The preparation method of the epimedium extract is as follows: (1) crushing dried epimedium leaves, adding water, mixing at a solid-liquid ratio of 1g:20-100mL, standing for 12-48h, filtering, mixing the filter residue with water at a ratio of 1g:5-30mL, placing in a 75-95°C water bath and stirring for 2-5h, then extracting with a 450-550W microwave for 15-45min, filtering, combining all filtrates, adding ethanol for precipitation, centrifuging, deproteinizing the precipitate, dialyzing, re-precipitating, centrifuging, and drying to obtain epimedium extract I; (2) adding methanol to the filter residue obtained in step (1) for reflux at a solid-liquid ratio of 1g:20-80mL, filtering, combining the filtrates, and drying the filtrates to obtain epimedium extract II;

[0019] The preparation method of the Schisandra chinensis extract comprises the following steps: slicing, drying and crushing the Schisandra chinensis fruit, adding petroleum ether or anhydrous ether at a solid-liquid ratio of 1 g:10-50 mL, soaking for 6-12 hours, placing in a water bath at 30-60° C. for ultrasonic treatment for 10-60 minutes, filtering and discarding the filtrate, and drying the residue for later use; (1) adding water to the residue at a solid-liquid ratio of 1 g:20-100 mL, standing for 12-48 hours, filtering, mixing the residue with water at a ratio of 1 g:10-50 mL, placing in a water bath at 70-90° C. for stirring for 2-5 hours, and then heating at 300-550 ° C. W for ultrasonic extraction, the ultrasonic time is 5 to 45 minutes, filtered, ethanol is added to the filtrate for precipitation, centrifuged, the precipitate is deproteinized, dialyzed, re-precipitated, centrifuged, and dried to obtain Schisandra extract I; (2) ethanol is added to the filter residue obtained in step (1), the solid-liquid ratio is 1g:20 to 80mL, stirred in a 50 to 60°C water bath for 1 to 3h, and then ultrasonic extraction is performed at 300 to 550W, the ultrasonic time is 5 to 25min, filtered, the filter residue is repeated for 2 to 3 times, the filtrate is combined, and the filtrate is dried to obtain Schisandra extract II;

[0020] The preparation method of the Gynostemma pentaphyllum extract comprises the following steps: (1) crushing dried Gynostemma pentaphyllum leaves into 40-60 meshes, adding water at a solid-liquid ratio of 1 g:10-80 mL, placing the mixture in a water bath at 70-90° C., stirring for 2-6 hours, filtering, adding ethanol to the filtrate for precipitation, centrifuging, deproteinizing the precipitate, dialyzing, re-precipitating, centrifuging, and drying to obtain Gynostemma pentaphyllum extract I; (2) adding ethanol to the filter residue obtained by filtering in step (1) and refluxing for 0.5-3 hours at a solid-liquid ratio of 1 g:10-50 mL, repeating the process 2-3 times, filtering and combining the filtrate, and drying the filtrate to obtain Gynostemma pentaphyllum extract II;

[0021] The preparation method of the psoralea corylifolia extract is as follows: adding petroleum ether or anhydrous ether to psoralea corylifolia powder at a solid-liquid ratio of 1g:10-50mL, stirring for 6-12h, then placing in a 30-60°C water bath for ultrasonication for 10-60min, filtering and discarding the filtrate, adding methanol to the filter residue after drying and soaking for 12-48h, the solid-liquid ratio is 1g:30-80mL, then ultrasonication at 300-350W for 10-35min, filtering, adding methanol to the filter residue for reflux for 15-60min, the solid-liquid ratio is 1g:30-50mL, filtering, combining the filtrate, and drying to obtain the psoralea corylifolia extract;

[0022] The preparation method of the Acanthopanax senticosus extract is as follows: (1) washing, drying and crushing the Acanthopanax senticosus leaves, defatting with petroleum ether or anhydrous ether, adding water after drying at a solid-liquid ratio of 1 g:20-80 mL, placing in a water bath at 70-90° C., stirring for 2-6 hours, filtering, adding ethanol to the filtrate for precipitation, centrifuging, and drying the precipitate for dialysis, ethanol re-precipitation, centrifuging, and drying to obtain Acanthopanax senticosus extract I; (2) adding ethanol to the filter residue obtained by filtering in step (1) and refluxing for 0.5-3 hours at a solid-liquid ratio of 1 g:10-50 mL, repeating 2-3 times, filtering and combining the filtrate, drying the filtrate and adding ethyl acetate for extraction 2-3 times, combining the ethyl acetate extracts, and drying to obtain Acanthopanax senticosus extract II, and continuously extracting the remaining filter residue with n-butanol for 1-2 times, combining the n-butanol extracts, and drying to obtain Acanthopanax sendicosus extract III;

[0023] The preparation method of the chicken bone incense extract is as follows: (1) grinding dried chicken bone incense, defatting with petroleum ether or anhydrous ether, adding water at a solid-liquid ratio of 1g:20-80mL, placing in a 70-90°C water bath, stirring for 2-6h, then ultrasonicating for 15-45min, filtering, adding ethanol to the filtrate for precipitation, centrifugation, dialysis, repeated precipitation, centrifugation, and drying to obtain chicken bone incense extract I; (2) adding ethanol to the filtered residue in step (1) and refluxing for 0.5-3h, with a solid-liquid ratio of 1g:10-50mL, repeating 2-3 times, filtering and combining the filtrate, adding ethyl acetate to the filtrate after drying for extraction 2-3 times, combining the ethyl acetate extracts and drying to obtain chicken bone incense extract II, and continuously extracting the remaining residue with n-butanol for 1-2 times, combining the n-butanol extracts and drying to obtain chicken bone incense extract III;

[0024] The preparation method of the Citrus aurantium extract is as follows: (1) crushing dried Citrus aurantium, adding petroleum ether or anhydrous ether with a solid-liquid ratio of 1g:10-50mL to extract 3-5 times, combining the extracts, and drying to obtain Citrus aurantium extract I; (2) continuously adding ethyl acetate to the solid extracted in step (1) for extraction 2-3 times, with a solid-liquid ratio of 1g:10-50mL, combining the extracts, and drying to obtain Citrus aurantium extract II; (3) continuously adding methanol to the solid extracted in step (2) for extraction 2-3 times, with a solid-liquid ratio of 1g:10-50mL, combining the extracts, and drying to obtain Citrus aurantium extract III;

[0025] The preparation method of the white peony root extract is as follows: (1) slicing, drying and crushing the white peony root, adding petroleum ether or anhydrous ether with a solid-liquid ratio of 1g:10-50mL to extract 3-5 times, combining the extracts, and drying to obtain red peony root extract I; (2) continuously adding 50% ethanol to the solid extracted in step (1) for extraction 2-3 times, with a solid-liquid ratio of 1g:10-50mL, combining the extracts, and drying to obtain white peony root extract II; (3) continuously adding 75% ethanol to the solid extracted in step (2) for extraction 2-3 times, with a solid-liquid ratio of 1g:10-50mL, combining the extracts, and drying to obtain white peony root extract III;

[0026] The preparation method of the red peony root extract comprises the following steps: (1) grinding dried red peony root, adding petroleum ether or anhydrous ether with a solid-liquid ratio of 1 g:10-50 mL, extracting for 3-5 times, combining the extracts, and drying to obtain red peony root extract I; (2) adding ethyl acetate to the solid extracted in step (1) for extraction for 2-3 times with a solid-liquid ratio of 1 g:10-50 mL, combining the extracts, and drying to obtain red peony root extract II; (3) adding 75% ethanol to the solid extracted in step (2) for extraction for 2-3 times with a solid-liquid ratio of 1 g:10-50 mL, combining the extracts, and drying to obtain red peony root extract III.

[0027] The Chinese medicine composition for invigorating qi and activating blood circulation of the present invention comprises 13 kinds of Chinese medicinal materials raw materials, including epimedium, schisandra, gynostemma pentaphyllum, psoralea corylifolia, acanthopanax senticosus, ginseng, licorice, radix scutellariae, immature bitter orange, dried orange peel, angelica, white peony root and red peony root, wherein the active ingredients of four kinds of Chinese medicinal materials, including epimedium, schisandra, gynostemma pentaphyllum and psoralea corylifolia, are extracted, and the epimedium extract II, schisandra extract II, gynostemma extract II and psoralea corylifolia extract with larger polarity are extracted. (5-10): (0.5-2): (3-8): (1-2) are the preferred compounding ratios. Compounding them as yang-tonifying and deficiency-tonifying ingredients can delay cardiovascular aging by treating the degeneration of the corresponding functions of the body caused by the deficiency of yin and yang, the deficiency of internal organs, and the depletion of essence, energy and spirit. The present invention extracts the active ingredients of Acanthopanax senticosus and compounds them with ginseng and liquorice as ingredients for strengthening and replenishing the spleen and qi. The present invention extracts the active ingredients of Acanthopanax senticosus and compounds them with ginseng and liquorice as ingredients for strengthening and replenishing the spleen and qi. The present invention extracts the active ingredients of white peony root and mixes them with dried tangerine peel as a component for regulating qi and soothing the liver, relieving depression and removing stagnation, breaking up qi and eliminating accumulation; the present invention extracts the active ingredients of white peony root and mixes them with angelica as a component for nourishing blood and improving qi and blood deficiency; the present invention extracts the active ingredients of red peony root as an auxiliary harmonizing component for clearing heat and cooling blood; in addition, the present invention extracts the water-soluble substances in five components of epimedium, schisandra, gynostemma pentaphyllum, psoralea corylifolia, acanthopanax senticosus and radix strychnifolia, and the obtained extracts are epimedium extract I, schisandra extract I, gynostemma pentaphyllum extract I, psoralea corylifolia extract I, acanthopanax senticosus extract I and radix strychnifolia extract I, which are mixed in the ratio of (0.1-0.5):(1-5):(5-10):(5-10):(2-5). The serum antioxidant activity of the obtained traditional Chinese medicine composition for invigorating qi and promoting blood circulation is within a preferred range, while the antioxidant activity of the composite composition exceeding this range is significantly reduced.

[0028] In a third aspect, the present invention provides a drug for treating cardiovascular aging, wherein the drug comprises a traditional Chinese medicine composition for invigorating qi and activating blood circulation.

[0029] Furthermore, the drug for treating cardiovascular aging also contains pharmaceutically acceptable excipients, including thickeners, fillers, adhesives, wetting agents, disintegrants, lubricants, etc., but are not limited to the types of pharmaceutical excipients listed above, and other types of pharmaceutical excipients not listed are also applicable;

[0030] Furthermore, the excipients include gelatin, sucrose, mannitol, sorbitol, mannitol, sorbitol, talc, silica gel, magnesium stearate, starch, dextrin, lactose, microcrystalline cellulose, polyethylene glycol, mucilage, povidone, cellulose derivatives, etc., but are not limited to the pharmaceutical excipients listed above. Other excipients not listed within the above-mentioned pharmaceutical excipient types are also applicable.

[0031] Furthermore, the drug for treating cardiovascular aging of the present invention is administered orally or by injection.

[0032] Furthermore, the dosage form of the drug for treating cardiovascular aging is a suspension, granules, capsules, powders, tablets, solutions, pills and / or injections, but is not limited to the dosage forms listed above. Other dosage forms not listed within the above dosage form range are also applicable.

[0033] The active effects of the traditional Chinese medicine composition for invigorating qi and activating blood circulation prepared by the present invention include anti-oxidation, anti-fatigue, inhibition of oxidative stress, reduction of inflammatory response, protection of mitochondrial function, improvement of cell proliferation activity, and the like, which play a role in delaying myocardial cell aging through multiple targets and pathways.

[0034] The indications of the traditional Chinese medicine composition for invigorating qi and activating blood circulation prepared by the present invention include qi deficiency, fatigue, blood stasis, yin deficiency, blood circulation, kidney deficiency, etc., but are not limited to the activities and effects listed above. Other activities and effects not listed within the scope of the above activities and effects are also applicable.

[0035] Compared with the prior art, the present invention has the following beneficial effects:

[0036] (1) The Chinese medicine composition for invigorating qi and promoting blood circulation of the present invention contains 13 kinds of Chinese medicinal materials, and the sources of the raw materials are abundant. The rationality of the compatibility between the components is confirmed through different compounding methods, and the efficacy of the ingredients from different Chinese medicinal materials is distinguished and clarified to a certain extent, which provides a certain theoretical basis for the preparation of subsequent Chinese medicine compound preparations.

[0037] (2) The Chinese herbal medicine composition for invigorating qi and promoting blood circulation of the present invention comprises 13 kinds of Chinese herbal medicine raw materials, and the active ingredients of 9 kinds of Chinese herbal medicine raw materials are extracted respectively. The active ingredients of different polarities are extracted respectively by using different solvents, and scientific combination is carried out through a large number of experiments to finally obtain the best formula. Compared with the prior art, the Chinese herbal medicine composition for invigorating qi and promoting blood circulation of the present invention has a more scientific formula and a better anti-cardiovascular aging activity effect.

[0038] (3) The Chinese herbal composition for invigorating qi and promoting blood circulation of the present invention can significantly increase the activity of the SOD antioxidant enzyme in serum and reduce the content of the lipid peroxidation product MDA in serum. For the D-galactose-induced leukemia, the SOD in serum can reach more than 135 U / mg after administration of the Chinese herbal composition for invigorating qi and promoting blood circulation, and MDA is reduced to 17.56 nmol / mg. It can be seen that the Chinese herbal composition for invigorating qi and promoting blood circulation prepared by the present invention has a good effect on the antioxidant activity of the body. At the same time, the Chinese herbal composition for invigorating qi and promoting blood circulation of the present invention can significantly promote the proliferation of macrophages in the concentration range of 25 to 100 ug / mL, slow down the aging rate of the body, and maintain the vitality of the body. DETAILED DESCRIPTION

[0039] The experimental methods in the following examples of the present invention, where no specific conditions are specified, are usually carried out under conventional conditions or under conditions recommended by the manufacturers. The various commonly used chemical reagents used in the examples are all commercially available products.

[0040] Unless otherwise defined, all technical and scientific terms used in the present invention have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0041] The terms "including" and "having" and any variations thereof of the present invention are intended to cover non-exclusive inclusions. For example, a process, method, device, product or equipment comprising a series of steps is not limited to the listed steps or modules, but may optionally include steps not listed, or may optionally include other steps inherent to these processes, methods, products or equipment.

[0042] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that these descriptions are exemplary only and are not intended to limit the scope of the present invention. In addition, in the following description, the description of known structures and technologies is omitted to avoid unnecessary confusion of the concept of the present invention.

[0043] The present invention is further described in the following examples, but the examples are not intended to limit the scope of protection of the present invention.

[0044] Some important cell lines used in the present invention are described in detail as follows:

[0045] Normal human prostate epithelial cells RWPE-1 were purchased from Shanghai Tongwei Biotechnology Co., Ltd.;

[0046] Human prostate cancer cell line LNCAP (androgen-sensitive) was purchased from Dalian Meilun Biotechnology Co., Ltd. LNCaPClone FGC cell line, which was derived from the left clavicular lymph node biopsy sample of a 50-year-old male patient with metastatic prostate cancer.

[0047] Human prostate cancer cells PC-3 (androgen-insensitive) were purchased from Dalian Meilun Biotechnology Co., Ltd. and originated from the bone marrow metastasis of a 62-year-old male with grade IV prostate cancer.

[0048] Example 1 Preparation of raw materials in the Chinese medicine composition for replenishing qi and promoting blood circulation

[0049] Preparation of Epimedium Extract I and II:

[0050] (1) The dried leaves of Epimedium were crushed, and then deionized water was added, mixed at a solid-liquid ratio of 1 g:50 mL, allowed to stand for 12 h, filtered, the filtrate was mixed with water at a ratio of 1 g:30 mL, placed in an 85° C. water bath and stirred for 2 h, then extracted with a microwave power of 550 W for 30 min, then filtered, all the filtrates were combined, 75% ethanol was added for precipitation, centrifuged, the precipitate was dried at low temperature, an appropriate amount was added to deionized water to prepare a 5% aqueous solution, and 1 / 5 volume of sevage reagent (V 氯仿 :V 正丁醇 =3:1), shake for 20 minutes, centrifuge, take the supernatant, add 10 times the weight of water, dialyze in water for 48 hours (molecular weight cutoff is 12KD-14KD), change the water every 4 hours, then add 75% ethanol to precipitate again until no precipitation occurs, centrifuge, and dry the precipitate at low temperature to obtain Epimedium extract I;

[0051] (2) adding methanol with a solid-liquid ratio of 1 g:50 mL to the residue obtained after microwave extraction in step (1) and performing reflux extraction for 30 min, filtering, repeating the above methanol reflux extraction step on the residue again, filtering, combining the two filtrates, and drying at low temperature to obtain Epimedium extract II;

[0052] Preparation of Schisandra Extracts I and II:

[0053] The fruit of Schisandra chinensis was cut into thin slices, dried and crushed at 50°C, petroleum ether was added at a solid-liquid ratio of 1g:30mL, and the mixture was soaked for 12h, then placed in a 35°C water bath for ultrasonic treatment for 40min, the filtrate was filtered and discarded, and the residue was used for later use after the solvent was evaporated; (1) deionized water was added to the residue at a solid-liquid ratio of 1g:50mL, the residue was allowed to stand for 48h, placed in a 85°C water bath and stirred for 2h, and then ultrasonic extraction was performed at 350W for 25min, filtered, 75% ethanol was added to the filtrate for precipitation, centrifuged, the precipitate was spin-dried at low temperature, deionized water was added to prepare an 8% aqueous solution, 1 / 4 volume of sevage reagent (V 氯仿 :V 正丁醇 =3:1), shake for 20 minutes, centrifuge, take the supernatant, add 10 times the weight of water, dialyze in water for 36 hours (molecular weight cutoff is 12KD-14KD), change the water every 4 hours, then add 75% ethanol to reprecipitate until no precipitation occurs, centrifuge, and dry the precipitate at low temperature to obtain Schisandra chinensis extract I;

[0054] (2) adding ethanol to the residue obtained after ultrasonic extraction in step (1) at a solid-liquid ratio of 1 g:80 mL, stirring in a 60° C. water bath for 1 h, then ultrasonically extracting at 420 W for 15 min, filtering, repeating the extraction step twice on the residue, combining the filtrates, and drying the filtrates at low temperature to obtain Schisandra chinensis extract II;

[0055] Preparation of Gynostemma pentaphyllum Extracts I and II:

[0056] (1) The dried leaves of Gynostemma pentaphyllum were crushed into 40 meshes, deionized water was added at a solid-liquid ratio of 1 g:80 mL, the mixture was placed in an 80°C water bath, stirred for 2 h, and then filtered. The filtrate was precipitated by adding 75% ethanol and centrifuged. After the precipitate was dried, deionized water was added to prepare a 5% aqueous solution, and 1 / 5 volume of sevage reagent (V 氯仿 :V 正丁醇 =3:1), shake for 20 minutes, centrifuge, take the supernatant, add 10 times the weight of water, dialyze in water for 48 hours (molecular weight cutoff is 12KD-14KD), change the water every 4 hours, then add 75% ethanol to reprecipitate until no precipitation occurs, centrifuge, and dry at low temperature to obtain Gynostemma pentaphyllum extract I;

[0057] (2) adding ethanol to the residue obtained by water extraction and filtration in step (1) and refluxing for 2 h, with a solid-liquid ratio of 1 g:40 mL, repeating twice, filtering and combining the filtrate, and drying the filtrate at low temperature to obtain Gynostemma pentaphyllum extract II;

[0058] Preparation of Psoralea corylifolia extract:

[0059] The dried psoralea corylifolia powder was added with petroleum ether at a solid-liquid ratio of 1g:40mL and stirred for 8h, then placed in a 35°C water bath and ultrasonicated (350W) for 20min, the filtrate was filtered and discarded, the filter residue was added with methanol and soaked for 48h, the solid-liquid ratio was 1g:80mL, then ultrasonicated at 350W for 35min, filtered, the filter residue was added with methanol and refluxed for 40min, the solid-liquid ratio was 1g:50mL, filtered, the two methanol filtrates were combined, and the low-temperature spin-dried extract was obtained;

[0060] Preparation of Acanthopanax Senticosus Extracts I, II and III:

[0061] (1) The dried Acanthopanax senticosus leaves were crushed, petroleum ether was added at a solid-liquid ratio of 1 g:30 mL, and the mixture was stirred for 2 h. The filtrate was filtered and discarded. After the solvent was evaporated from the filter residue, deionized water was added at a solid-liquid ratio of 1 g:50 mL, and the mixture was placed in an 80° C. water bath and stirred for 2 h. The mixture was then filtered, and 75% ethanol was added to the filtrate for precipitation and centrifugation. The precipitate was dried at low temperature and then added with 10 times the mass of water and dialyzed in water for 48 h (molecular weight cutoff of 8 KD). The water was changed every 4 h, and then 75% ethanol was added for repeated precipitation until no precipitation was observed. The mixture was centrifuged and dried at low temperature to obtain Acanthopanax senticosus extract I.

[0062] (2) adding ethanol to the filter residue obtained by filtering in step (1) in a water bath and refluxing for 2 h, with a solid-liquid ratio of 1 g:50 mL, repeating twice, filtering and combining the filtrate, and spinning the filtrate at low temperature to obtain an extract, adding ethyl acetate twice the mass of the extract to the extract for extraction three times, combining the ethyl acetate extracts, and spinning at low temperature to obtain Acanthopanax senticosus extract II, and continuing to add n-butanol twice the mass of the extract to the extract for extraction twice, combining the n-butanol extracts, and spinning at low temperature to obtain Acanthopanax senticosus extract III;

[0063] Preparation of chicken bone fragrance extracts I, II, and III:

[0064] (1) The dried chicken bone incense was crushed, petroleum ether was added at a solid-liquid ratio of 1g:30mL, and stirred for 2h, the filtrate was filtered and discarded, and after the solvent was evaporated from the filter residue, deionized water was added at a solid-liquid ratio of 1g:50mL, placed in an 80°C water bath, stirred for 2h, and then ultrasonicated at 350W for 30min, filtered, 75% ethanol was added to the filtrate for precipitation and centrifugation, and the precipitate was spin-dried at low temperature and then 10 times the weight of water was added to dialyze in water for 36h (molecular weight cut-off was 8KD), the water was changed every 4h, and then 75% ethanol was added to precipitate again until no precipitation was precipitated, centrifuged, and spin-dried at low temperature to obtain the chicken bone incense extract I;

[0065] (2) adding ethanol to the filter residue after ultrasonic filtration in step (1) and refluxing for 1h, the solid-liquid ratio is 1g:50mL, and the filter residue is repeatedly refluxed once after filtration, filtered, and the filtrate is combined. The filtrate is spin-dried at low temperature to obtain an extract, and ethyl acetate is added to the extract with 2 times the mass of the extract to fully mix and extract 3 times, filtered, and the ethyl acetate extract is combined and spin-dried at low temperature to obtain Jiguxiang extract II, and n-butanol is added to the extract with 2 times the mass of the extract to extract 2 times, and the n-butanol extract is filtered and combined and spin-dried at low temperature to obtain Jiguxiang extract III;

[0066] Preparation of Citrus aurantium extracts I, II and III:

[0067] (1) crushing the dried Citrus aurantium immaturus, adding petroleum ether with a solid-liquid ratio of 1 g:30 mL, extracting three times, filtering, combining the filtrate, and drying at low temperature to obtain Citrus aurantium immaturus extract I;

[0068] (2) adding ethyl acetate to the filter residue of step (1) for extraction three times, with a solid-liquid ratio of 1 g:30 mL, filtering, combining the filtrate, and drying at low temperature to obtain the Citrus aurantium extract II;

[0069] (3) adding methanol to the filter residue of step (2) for extraction twice, with a solid-liquid ratio of 1 g:30 mL, filtering, combining the filtrate, and drying at low temperature to obtain the Citrus aurantium extract III;

[0070] Preparation of white peony root extracts I, II and III:

[0071] (1) The washed white peony root was sliced, dried, and crushed, and petroleum ether with a solid-liquid ratio of 1 g:30 mL was added and extracted three times, filtered, the filtrate was combined, and the red peony root extract I was obtained by spin drying at low temperature;

[0072] (2) adding 50% ethanol to the residue of step (1) for extraction three times, with a solid-liquid ratio of 1 g:30 mL, filtering, combining the filtrate, and drying at low temperature to obtain white peony root extract II;

[0073] (3) Add 75% ethanol to the residue in step (2) and extract twice with a solid-liquid ratio of 1 g:20 mL, filter, combine the filtrate, and dry at low temperature to obtain white peony root extract III.

[0074] Preparation of red peony root extracts I, II and III:

[0075] (1) grinding the dried red peony root, adding petroleum ether with a solid-liquid ratio of 1 g:30 mL, extracting three times, filtering, combining the filtrate, and drying at low temperature to obtain red peony root extract I;

[0076] (2) adding ethyl acetate to the filter residue of step (1) for extraction three times, with a solid-liquid ratio of 1 g:20 mL, filtering, combining the filtrate, and drying at low temperature to obtain red peony root extract II;

[0077] (3) Add 75% ethanol to the filter residue in step (2) and extract twice with a solid-liquid ratio of 1 g:20 mL. Filter, combine the filtrate, and dry at low temperature to obtain red peony root extract III.

[0078] Example 2 Chinese medicine composition formula 1 for replenishing qi and promoting blood circulation

[0079] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0080]

[0081]

[0082] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0083] Example 3 Chinese medicine composition formula 2 for replenishing qi and promoting blood circulation

[0084] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0085]

[0086]

[0087] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0088] Example 4 Chinese medicine composition formula 3 for replenishing qi and promoting blood circulation

[0089] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0090]

[0091]

[0092] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0093] Example 5 Chinese medicine composition formula 4 for replenishing qi and promoting blood circulation

[0094] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0095]

[0096]

[0097] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0098] Example 6 Chinese medicine composition formula 5 for replenishing qi and promoting blood circulation

[0099] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0100]

[0101]

[0102] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0103] Example 7 Chinese medicine composition formula 6 for replenishing qi and promoting blood circulation

[0104] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0105]

[0106]

[0107] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0108] Example 8 Chinese medicine composition formula 7 for replenishing qi and promoting blood circulation

[0109] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0110] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0111] Example 9 Chinese medicine composition formula 8 for replenishing qi and promoting blood circulation

[0112] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0113]

[0114]

[0115] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0116] Example 10 Chinese medicine composition formula 9 for replenishing qi and promoting blood circulation

[0117] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0118]

[0119]

[0120] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0121] Example 11 Chinese medicine composition formula 10 for replenishing qi and promoting blood circulation

[0122] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0123]

[0124]

[0125] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0126] Example 12 Chinese medicine composition formula 11 for replenishing qi and promoting blood circulation

[0127] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0128]

[0129]

[0130] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0131] Example 13 Chinese medicine composition formula 12 for replenishing qi and promoting blood circulation

[0132] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0133]

[0134]

[0135] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0136] Example 14 Chinese medicine composition formula 13 for replenishing qi and promoting blood circulation

[0137] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0138]

[0139]

[0140] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0141] Example 15 Chinese medicine composition formula 14 for replenishing qi and promoting blood circulation

[0142] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0143]

[0144]

[0145] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0146] Example 16 Chinese medicine composition formula 15 for replenishing qi and promoting blood circulation

[0147] The formula of the Chinese medicine composition for invigorating qi and promoting blood circulation in this embodiment is as follows by weight:

[0148]

[0149]

[0150] The above ingredients were crushed and ground into 60 mesh powder and mixed thoroughly.

[0151] Test Example 1: Determination of the in vivo antioxidant activity of a traditional Chinese medicine composition for invigorating qi and activating blood circulation

[0152] (1) Experimental subjects and modeling: 198 SPF Kunming mice, half male and half female, were selected and adaptively fed for 7 days and randomly divided into 15 experimental groups, 1 blank control group, 1 model control group, and 1 positive control group, with 11 mice in each group. D-galactose was administered to the 15 experimental groups, 1 model control group, and 1 positive drug control group to establish an oxidative damage model. Blood samples were collected 30 days later to determine whether the modeling was successful. If unsuccessful, new mice were purchased for feeding and modeling steps until the modeling was successful;

[0153] (2) Experimental administration: 15 groups of traditional Chinese medicine compositions for invigorating qi and activating blood circulation and positive drug (vitamin C) prepared in Examples 2 to 16 were administered by intragastric administration at a rate of 0.4 g / kg per day for 8 consecutive weeks. During the experiment, the animals were free to drink water and eat;

[0154] (3) Determination of antioxidant indexes: Blood from each group of mice was centrifuged at 3500 r / min for 10 min at 4°C and the supernatant was taken for determination. The levels of superoxide dismutase (SOD) (WST-1 method) and malondialdehyde (MDA) (TBA method) in serum were measured according to the instructions of the kits. The kits were purchased from Nanjing Jiancheng Bioengineering Research Institute Co., Ltd.

[0155] The results obtained by the above experimental method and calculation method are shown in Table 1;

[0156] Table 1: Effect of the Chinese medicine composition for replenishing qi and activating blood circulation on the in vivo antioxidant indexes ( n=10)

[0157]

[0158]

[0159] Note: *P<0.05, **P<0.01, all compared with the blank control group; # P < 0.05, ## P < 0.01, compared with the model control group.

[0160] Experimental groups 1 to 15 in Table 1 are the results of 15 groups of Chinese medicine compositions for invigorating qi and promoting blood circulation prepared in Examples 2 to 16 after administration. It can be seen from the results in Table 1 that compared with the blank control group, the SOD value of the model control group decreased significantly, and the MDA value increased significantly. The statistical results showed extremely significant differences (P<0.01), which showed that the model was successfully established; compared with the model control group, the SOD of experimental groups 1 to 15 increased significantly, and the statistical results showed significant differences (P<0.05). From the analysis of the statistical results, the Chinese medicine compositions for invigorating qi and promoting blood circulation prepared in experimental groups 1 to 5 increased the SOD antioxidant enzyme in serum compared with experimental groups 6 to 15. The activity value is more significant, which shows that the activity difference of different extract parts of different medicinal materials is large, and the antioxidant activity of the Chinese medicine composition for invigorating qi and promoting blood circulation of the present invention needs to be within a specific range of formula to exert better efficacy; on the other hand, the MDA of the experimental groups 1 to 15 of the present invention decreased significantly compared with the model control group, and the statistical results showed significant differences, especially the Chinese medicine composition for invigorating qi and promoting blood circulation prepared by the experimental groups 1 to 5 had a more significant effect on reducing the content of MDA, a lipid peroxidation product in serum. It can be seen that the 5 groups of Chinese medicine compositions for invigorating qi and promoting blood circulation prepared in Examples 2 to 6 of the present invention have better antioxidant activity effects on the body.

[0161] In general, the traditional Chinese medicine composition for invigorating qi and promoting blood circulation of the present invention can significantly improve the body's antioxidant activity, thereby slowing down the body's aging speed and maintaining the body's vitality.

[0162] Experimental Example 2: Effect of the Chinese herbal composition for invigorating qi and activating blood circulation on macrophage proliferation

[0163] (1) Inoculation: Logarithmic phase cells were collected and mouse macrophages RAW264.7 (Shanghai Cell Bank, Chinese Academy of Sciences) in the logarithmic growth phase were prepared into 1×10 5 Cell suspensions of 100 μL / mL were inoculated into cell culture plates, and 100 μL of cell culture medium was added to each well;

[0164] (2) Culture: The cells were cultured in a cell culture incubator at 37°C and 5% CO2 for 24 h;

[0165] (3) Dosing: A certain amount of the Chinese medicine composition sample for invigorating qi and promoting blood circulation was added with DMSO to prepare a mixed solution with a concentration of 25, 50, and 100 ug / mL, and the mixture was shaken for 20 min, filtered, and centrifuged. The supernatant was taken and 15 groups of Chinese medicine composition samples for invigorating qi and promoting blood circulation (Example 2 to Example 16) were added to the cultured cells separately to replace the solution, 100 uL per well, and each group was set with 3 replicate wells;

[0166] (4) Color development: After 48 hours, add 10uL of MTT solution to each well and incubate in an incubator for 4 hours. Terminate the culture, discard the supernatant in the well, add 100uL of DMSO to each well, and shake for 10 minutes to fully dissolve the crystals.

[0167] (1) Colorimetry: Measure the OD value at a wavelength of 570 nm using an ELISA reader;

[0168] (2) Data processing and analysis;

[0169] The cell proliferation rate (%) in this experiment was calculated as follows:

[0170]

[0171] The results of calculating the effect of the traditional Chinese medicine composition for invigorating qi and activating blood circulation on the proliferation rate of macrophages according to the above experimental method are shown in Table 2 below;

[0172] Table 2: Effect of the Chinese herbal medicine composition for invigorating qi and activating blood circulation on the proliferation rate (%) of macrophages n=3)

[0173]

[0174] Note: # P < 0.05, ## P<0.01, compared with the blank control group.

[0175] As can be seen from the results in Table 2, the 15 groups of traditional Chinese medicine compositions for invigorating qi and activating blood circulation in experimental groups 1 to 15 have significant proliferation activity on mouse macrophages RAW264.7 within the concentration range of 25 to 100 ug / mL. The proliferation effect of the traditional Chinese medicine composition for invigorating qi and activating blood circulation prepared by the present invention on cells increases with the increase of concentration, and there is a significant difference in the statistical results compared with the blank control group (P<0.05). From the analysis of the data statistical results, the traditional Chinese medicine composition for invigorating qi and activating blood circulation prepared by experimental groups 1 to 5 has higher cell proliferation activity than experimental groups 6 to 15, while the cell proliferation activity of experimental groups 6 to 8 is lower than that of experimental groups 9 to 10, indicating that the components extracted by organic solvents of Epimedium, Schisandra chinensis, Gynostemma pentaphyllum and Psoralea corylifolia have the greatest effect on the proliferation activity of mouse macrophages RAW264.7 within a specific proportion range.

[0176] It should be noted that the specific features, structures, materials or characteristics described in this specification can be combined arbitrarily. In order to make the description concise, not all possible combinations of the various technical features in the above embodiments are described. If there is no contradiction, technicians in this field can combine and combine the different embodiments and features of different embodiments described in this specification.

[0177] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A Chinese medicine composition for invigorating qi and promoting blood circulation, characterized in that: The raw materials of the traditional Chinese medicine composition are 0.1-0.5 parts by weight of epimedium extract I, 5-10 parts by weight of epimedium extract II, 1-5 parts by weight of schisandra extract I, 0.5-2 parts by weight of schisandra extract II, 5-10 parts by weight of gynostemma extract I, 3-8 parts by weight of gynostemma extract II, 1-2 parts by weight of psoralea corylifolia extract, 5-10 parts by weight of Acanthopanax senticosus extract I, 5-10 parts by weight of Acanthopanax senticosus extract II, 0.5-2.5 parts by weight of Acanthopanax senticosus extract III, 20-55 parts by weight of ginseng, 5-15 parts by weight of liquorice, 2-5 parts by weight of scutellaria baicalensis extract I, 2-5 parts by weight of scutellaria baicalensis extract II, 1.5-2.5 parts by weight of scutellaria baicalensis extract III, 1-3 parts by weight of immature citrus aurantium extract I, 0.5-2 parts by weight of immature citrus aurantium extract II, and 3-8 parts by weight of scutellaria baicalensis extract III. 2-5 parts by mass, tangerine peel 3-8 parts by mass, angelica 15-40 parts by mass, white peony root extract I 1-5 parts by mass, white peony root extract II 5-15 parts by mass, white peony root extract III 1-3 parts by mass, red peony root extract I 0.1-0.5 parts by mass, red peony root extract II 0.1-2 parts by mass, red peony root extract III 0.5-2 parts by mass; The raw materials of the Chinese medicine composition are prepared by the following steps: The preparation method of the epimedium extract is as follows: (1) crushing dried epimedium leaves, adding water, standing for 12 to 48 hours, filtering, adding water to the filter residue, mixing, placing in a water bath at 75 to 95° C. and stirring for 2 to 5 hours, then microwave treatment, filtering, combining all filtrates, adding ethanol to precipitate, centrifuging, deproteinizing the precipitate, dialyzing, re-precipitating, centrifuging, and drying to obtain epimedium extract I; (2) adding methanol to the filter residue obtained in step (1) for reflux, filtering, and drying the filtrate to obtain epimedium extract II; The preparation method of the Schisandra chinensis extract comprises the following steps: slicing, drying and crushing the Schisandra chinensis fruit, adding petroleum ether or anhydrous ether to soak for 6 to 12 hours, ultrasonically treating, filtering and discarding the filtrate, and drying the filter residue for later use; (1) adding water to the filter residue and letting it stand for 12 to 48 hours, filtering, mixing the filter residue with water and placing it in a water bath at 70 to 90° C. and stirring it for 2 to 5 hours, then ultrasonically treating, filtering, adding ethanol to the filtrate for precipitation and centrifugation, deproteinizing the precipitate, dialyzing, re-precipitating, centrifuging and drying to obtain Schisandra chinensis extract I; (2) adding ethanol to the filter residue obtained in step (1), stirring it in a water bath at 50 to 60° C. for 1 to 3 hours, then ultrasonically treating, filtering, and drying the filtrate to obtain Schisandra chinensis extract II; The preparation method of the Gynostemma pentaphyllum extract comprises: (1) grinding dried Gynostemma pentaphyllum leaves, adding water, placing in a water bath at 70-90° C. and stirring for 2-6 hours, then filtering, adding ethanol to the filtrate for precipitation, centrifuging, deproteinizing the precipitate, dialysis, reprecipitation, centrifuging, and drying to obtain Gynostemma pentaphyllum extract I; (2) adding ethanol to the filter residue obtained by filtering in step (1), refluxing, filtering and combining the filtrate, and drying the filtrate to obtain Gynostemma pentaphyllum extract II; The preparation method of the psoralea corylifolia extract comprises: adding petroleum ether or anhydrous ether to psoralea corylifolia powder and stirring for 6 to 12 hours, then placing the mixture in a water bath at 30 to 60° C. for ultrasonic treatment, filtering, drying the filter residue and then adding methanol to soak the mixture for 12 to 48 hours, then ultrasonic treatment, filtering, adding methanol to the filter residue for reflux, filtering, and drying the filtrate to obtain the psoralea corylifolia extract; The preparation method of the Acanthopanax senticosus extract is as follows: (1) washing, drying and crushing Acanthopanax senticosus leaves, defatting with petroleum ether or anhydrous ether, adding water after drying and placing in a water bath at 70-90°C, stirring for 2-6 hours, filtering, adding ethanol to the filtrate for precipitation and centrifugation, drying the precipitate, dialyzing, re-precipitating with ethanol, centrifuging and drying to obtain Acanthopanax senticosus extract I; (2) adding ethanol to the filter residue obtained by filtering in step (1) for reflux, filtering, drying the filtrate and adding ethyl acetate for extraction, drying the ethyl acetate extract to obtain Acanthopanax senticosus extract II, further extracting the remaining filter residue with n-butanol, and drying the n-butanol extract to obtain Acanthopanax senticosus extract III; The preparation method of the chicken bone incense extract is as follows: (1) grinding dried chicken bone incense, defatting with petroleum ether or anhydrous ether, adding water, placing in a water bath at 70-90° C. and stirring for 2-6 hours, then ultrasonically treating, filtering, adding ethanol to the filtrate for precipitation, centrifugation, dialysis, repeated precipitation, centrifugation, and drying to obtain chicken bone incense extract I; (2) adding ethanol to the filtered residue in step (1) for reflux, filtering, drying the filtrate, adding ethyl acetate for extraction, drying the ethyl acetate extract to obtain chicken bone incense extract II, and continuously extracting the remaining filter residue with n-butanol, and drying the n-butanol extract to obtain chicken bone incense extract III; The preparation method of the Citrus aurantium extract comprises: (1) crushing dried Citrus aurantium, adding petroleum ether or anhydrous ether for extraction, and drying the extract to obtain Citrus aurantium extract I; (2) adding ethyl acetate to the solid after extraction in step (1) for extraction, and drying the extract to obtain Citrus aurantium extract II; (3) adding methanol to the solid after extraction in step (2) for extraction, and drying the extract to obtain Citrus aurantium extract III; The preparation method of the white peony root extract comprises: (1) slicing, drying and crushing the white peony root, adding petroleum ether or anhydrous ether for extraction, and drying the extract to obtain red peony root extract I; (2) adding 50% ethanol to the solid after extraction in step (1) for extraction, and drying the extract to obtain white peony root extract II; (3) adding 75% ethanol to the solid after extraction in step (2) for extraction, and drying the extract to obtain white peony root extract III; The preparation method of the red peony root extract comprises: (1) crushing dried red peony root, adding petroleum ether or anhydrous ether for extraction, and drying the extract to obtain red peony root extract I; (2) adding ethyl acetate to the solid after extraction in step (1) for extraction, and drying the extract to obtain red peony root extract II; (3) adding 75% ethanol to the solid after extraction in step (2) for extraction, and drying the extract to obtain red peony root extract III.

2. The Chinese medicine composition for invigorating qi and promoting blood circulation according to claim 1, characterized in that: The traditional Chinese medicine composition for invigorating qi and promoting blood circulation further comprises pharmaceutically acceptable excipients.

3. A method for preparing the Chinese medicine composition for invigorating qi and promoting blood circulation according to claim 1, comprising the following steps: The raw materials of the Chinese medicine composition according to claim 1 are ground into powder, and then fully mixed to obtain a Chinese medicine composition for invigorating qi and activating blood circulation.

4. A medicine comprising the Chinese medicine composition for invigorating qi and activating blood circulation according to claim 1, characterized in that: The dosage form of the drug is suspension, granules, capsules, powders, tablets, solutions, pellets and / or injections.

Images