A white enoki mushroom and its application

Abstract

The invention relates to a white enoki mushroom 3W4 (Flammulina filiformis), with a preservation number of GDMCC No. 64488, which has fast mycelium growth speed, early primordium formation, fast stipe elongation and shortened growth period, and can be used for enoki mushroom cultivation and breeding.

Description

Technical Field

[0001] The invention relates to the biological field, and in particular to a white golden needle mushroom and application thereof. Background Art

[0002] Flammulina filiformis, also known as the mushroom, the mushroom, the mushroom, the winter mushroom, the hairy money mushroom, etc., is an important edible and medicinal mushroom. Flammulina filiformis is delicious, smooth, nutritious, and rich in essential amino acids, vitamins, unsaturated fatty acids, and dietary fiber for humans. In addition, Flammulina filiformis also contains a large number of bioactive substances that are beneficial to health, including fungal polysaccharides, fungal immunomodulatory proteins, flavonoids, terpenes, glycosides, and phenolic compounds. It has specific functions in anti-tumor, lowering blood pressure, lowering cholesterol, anti-oxidation, and delaying aging. It also has a certain effect on the treatment of liver and allergic respiratory diseases.

[0003] At present, the domestic market for Flammulina velutipes is dominated by white varieties, accounting for more than 90%. Most of the existing white Flammulina velutipes strains have a long growth period and low yield and biological conversion efficiency. Therefore, it is necessary to find white Flammulina velutipes strains with a shorter growth period and higher utilization rate. Summary of the invention

[0004] The invention aims to provide a white enoki mushroom, which is named as Flammulina filiformis 3W4 and has a deposit number of GDMCC No.64488.

[0005] Another object of the present invention is to provide the use of the white Flammulina velutipes of the present invention in the cultivation and breeding of Flammulina velutipes.

[0006] Unless otherwise specified, when the present invention relates to the percentage between liquids, the percentage is volume / volume percentage; when the present invention relates to the percentage between liquids and solids, the percentage is volume / weight percentage; when the present invention relates to the percentage between solids and liquids, the percentage is weight / volume percentage; the rest are weight / weight percentages.

[0007] Compared with the prior art, the beneficial technical effects of the present invention include:

[0008] The white Flammulina velutipes scientifically screened out by the present invention has the advantages of fast mycelium growth speed, early primordium formation, fast stipe elongation, short growth period, high yield and high biological conversion rate, small difference between bottles, uniform mushroom production, high consistency, and is suitable for a factory-based large-scale production mode of one-time harvesting, which can greatly improve production efficiency and reduce costs, can be used for the cultivation and breeding of Flammulina velutipes, and has broad market prospects.

[0009] Biomaterial Deposit

[0010] The white enoki mushroom described in the present invention is named Flammulina filiformis, which was submitted for preservation on April 3, 2024, with the preservation number: GDMCC NO: 64488, the preservation unit: Guangdong Microbiological Culture Collection Center, and the preservation address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou City, Guangdong Province. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 Study on the mycelium growth rate of white Flammulina velutipes of the present invention;

[0012] Figure 2 The invention relates to a study on the factory-based bag-loading results of the white Flammulina velutipes. DETAILED DESCRIPTION

[0013] The present invention will be described below with reference to examples, but the present invention is not limited to the examples.

[0014] The PDA medium used in the embodiment is formulated as follows: 10 g potato, 20 g glucose, 15 g agar, and distilled water added to 1 L.

[0015] The cultivation medium used in the embodiment is formulated as follows: 80 g of cottonseed hulls, 10 g of wheat bran, 8 g of corn flour, 2 g of lime, and 150 g of water. Each cultivation bottle contains 750 g of the cultivation medium.

[0016] The white Flammulina velutipes F4 in the embodiment was purchased from Changhong Agricultural Biotechnology Co., Ltd., and the light yellow Flammulina velutipes Chuanjin No. 3 was purchased from Yuechi County Wanpeng Fungus Development Co., Ltd.

[0017] Example 1 Screening of white Flammulina velutipes strains of the present invention

[0018] The spore prints of white Flammulina velutipes F4 and light yellow Flammulina velutipes Chuanjin No. 3 were collected with sterile water, gradiently diluted and coated with PDA medium, cultured at 25°C in the dark for 4 days, and the monospore strains were isolated. The strains without lock-like union were detected under a microscope for subsequent hybridization experiments. A white Flammulina velutipes strain 3W4 was obtained using the single hybridization technique. After its tissue was separated, it was identified. The ITS sequence of strain 3W4 was amplified using ITS4 and ITS5 primers, and the Flammulina velutipes ITS sequence (accession number OK632275.1) was aligned to the Flammulina velutipes ITS sequence in NCBI, with a sequence identity of 99.56%.

[0019] After identification, the strain is Flammulina filiformis 3W4, which was submitted for deposit on April 3, 2024, with the deposit number: GDMCC NO: 64488. The deposit unit is Guangdong Provincial Microbiological Culture Collection Center, and the deposit address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou City, Guangdong Province.

[0020] Example 2 Research on the white Flammulina velutipes of the present invention

[0021] White Flammulina velutipes F4 and white Flammulina velutipes 3W4 were inoculated into PDA medium, cultured at 25°C for 7 days, and four groups were set up:

[0022] Experimental group 1: The edge hyphae (5 mm in diameter) of white Flammulina velutipes 3W4 were taken using a 0.5 cm diameter borer and inoculated onto PDA medium and cultured at 25° C. in the dark for 7 days.

[0023] Control group 1: Use a 0.5 cm diameter puncher to take the edge hyphae (5 mm in diameter) of white Flammulina velutipes F4, inoculate it on PDA medium, and culture it at 25° C. in the dark for 7 days.

[0024] Experimental group 2: The edge hyphae (5 mm in diameter) of white Flammulina velutipes 3W4 were taken using a 0.5 cm diameter puncher and inoculated onto the culture medium, and cultured at 25° C. in the dark for 14 days.

[0025] Control group 2: Use a 0.5 cm diameter puncher to take the edge hyphae (5 mm in diameter) of white Flammulina velutipes F4, inoculate it on the cultivation medium, and culture it at 25°C in the dark for 14 days.

[0026] The mycelial growth rate of each group was observed. Figure 1 , the mycelial growth rate of strain 3W4 was faster than that of F4.

[0027] Example 3 Study on the factory-based bagging of Flammulina velutipes of the present invention

[0028] White Flammulina velutipes 3W4 and White Flammulina velutipes F4 were inoculated into PDA medium and cultured at 25°C for 7 days. Mycelium blocks (2cm*2cm) of both were directly inoculated into the culture medium, and 30 bottles were inoculated for each.

[0029] The culture conditions are as follows: the temperature is 25℃ in the spawning stage, away from light, and the relative humidity of the environment is about 70%; after the mycelium fills the bottle, the spawn is moved to the low-temperature fruiting room, the temperature is about 15℃, the relative humidity of the ambient air is about 95%, and the carbon dioxide concentration is about 3000ppm. After a large number of primordia are formed, the temperature is adjusted to 7℃, the carbon dioxide concentration is about 8000ppm; the light intensity is about 50 lux. When the fruiting body grows to 3-4cm, a plastic tube is put around the fruiting body. The fruiting body reaches the harvest standard when the length is about 17cm. The fruiting results are shown in Figure 2 After harvesting, the fresh weight, dry weight and yield of Flammulina velutipes were recorded, and the bioconversion rate was calculated according to the biological conversion rate = (fresh weight of Flammulina velutipes / dry weight) * 100%.

[0030] The observation results show that the mycelium of white Flammulina velutipes 3W4 fills the bottle after 20 days of cultivation, a large number of primordia are formed after 6 days of scratching, and the harvest standard is reached after 45 days of cultivation. The overall growth period is 5-7 days earlier than that of white Flammulina velutipes F4. It has a higher average yield and biological conversion rate than F4 (the average yield of 3W4 per bottle is 462.0g, and the biological conversion rate is 154%), with smaller differences between bottles, more uniform mushroom production, and higher consistency. The factory-based large-scale production model suitable for one-time harvesting can greatly improve production efficiency, reduce costs, and has broad market prospects.

[0031] The above description of the specific embodiments of the present invention does not limit the present invention. Those skilled in the art may make various changes or modifications based on the present invention. As long as they do not depart from the spirit of the present invention, they should all fall within the scope of protection of the claims of the present invention.

Claims

1. A white enoki mushroom ( Flammulina filiformis ) 3W4, the deposit number is GDMCC No. 64488.

2. Application of the white Flammulina velutipes as claimed in claim 1 in the cultivation and breeding of Flammulina velutipes.

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