Baoyuan decoction for relieving fatigue and regulating immunity and preparation method thereof

Abstract

The invention discloses a Baoyuan decoction for relieving fatigue and regulating immunity and a preparation method thereof, and relates to the technical field of traditional Chinese medicine. The preparation method comprises: crushing ginseng, astragalus, licorice and cinnamon to obtain raw material A; mixing raw material A with extraction solvent 1, adding mixed enzyme, extracting, filtering to obtain extract 1 and filter residue 1; mixing filter residue 1 with water to obtain filter residue liquid, stirring and passing ozone treatment, filtering, washing, drying, and obtaining raw material B; mixing raw material B with extraction solvent 2, extracting, and combining extracts to obtain extract 2; mixing extract 1 and extract 2 uniformly, centrifuging, taking supernatant, adding water, and obtaining the Baoyuan decoction for relieving fatigue and regulating immunity. The present invention optimizes the extraction process, improves the efficacy, obtains better anti-fatigue effect and immunity improvement effect, and reduces the dosage of medication and improves patient compliance.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a Baoyuan decoction for relieving fatigue and regulating immunity and a preparation method thereof. Background Art

[0002] The pressure of work and life often makes many people prone to fatigue. The US Centers for Disease Control and Prevention defines Chronic Fatigue Syndrome (CFS) as a disease with long-term extreme fatigue as the main symptom, accompanied by insomnia, forgetfulness, muscle and joint pain, mental and neurological abnormalities and other symptoms.

[0003] At the same time, fatigue and decreased immunity are inseparable. Physical fatigue, lack of sleep or overwork will increase the burden on the autonomic nerves, and will also have adverse effects on the endocrine system and the immune system, thereby causing decreased immunity. Immunity is the body's own defense mechanism. It is the body's ability to identify and eliminate any foreign bodies (viruses, bacteria, etc.) that invade from the outside, deal with aging, damaged, dead, and degenerated self-cells, and identify and deal with mutant cells and virus-infected cells in the body. A body with low immunity is prone to infection or cancer. Biologically speaking, it refers to the ability to resist the development of disease. Modern immunology believes that immunity is the body's physiological response to identify and exclude foreign bodies.

[0004] From the perspective of traditional Chinese medicine, excessive emotional fluctuations such as joy, anger, sorrow, thinking, fear, and worry, or excessive hunger, overwork, or invasion of the body by external evils such as wind, cold, heat, dryness, and fire, can all cause meridian blockage, qi and blood to stagnate, the immune system to be damaged, and its function to decline, which will eventually lead to disease. Modern life is busy and tense, people get up early and go to bed late, and the quality of sleep is poor. They are prone to fatigue, headaches, chest tightness, and bloating. Women are prone to irregular menstruation. Traditional Chinese medicine calls the above symptoms liver qi stagnation. If accompanied by oral ulcer inflammation, angular cheilitis, etc., it means liver fire. The busy and tense life disrupts the body's physiological laws and weakens immunity.

[0005] Therefore, it is increasingly important to develop traditional Chinese medicine prescriptions that can relieve fatigue and enhance immunity.

[0006] Chinese patent application CN202310987979.5 discloses a new prescription and preparation method of Baoyuan Decoction for replenishing vital energy, tolerating hypoxia, and regulating chronic fatigue, which belongs to the field of traditional Chinese medicine technology. The invention cooperates ginseng, astragalus, licorice, cinnamon, and rhodiola with each other, and compatibility of medicinal materials is carried out according to the principle of monarch, minister, assistant and envoy. Ginseng and astragalus are monarch medicines, rhodiola is minister medicine, and licorice is used to reconcile the medicinal properties. Cinnamon leads fire back to the origin, relieves deficiency heat, and replenishes kidney yang. The compatibility is reasonable, and it has good functions of replenishing vital energy, tolerating hypoxia, and regulating chronic fatigue, and it is non-dependent and has low side effects. However, the composition has no clear effect of improving immunity, and cannot simultaneously solve the problem of decreased immunity.

[0007] Based on this, the present invention provides a Baoyuan Decoction for relieving fatigue and regulating immunity and a preparation method thereof. Ginseng, astragalus, licorice and cinnamon are used in combination, and the extraction process is optimized to improve the efficacy of the medicine, thereby obtaining better anti-fatigue and immunity-enhancing effects, while reducing the dosage of the medicine and improving patient compliance. Summary of the invention

[0008] The purpose of the present invention is to provide a Baoyuan decoction for relieving fatigue and regulating immunity and a preparation method thereof, which has good anti-fatigue effect and immunity-enhancing effect, realizes the reduction of prescription and enhancement of efficacy of traditional Chinese medicine, improves the safety of taking, reduces the drug dosage, improves the storage stability, optimizes the preparation process, and increases the content of effective ingredients.

[0009] To achieve the above-mentioned object of the invention, the technical solution of the present invention is as follows:

[0010] In one aspect, the present invention provides a Baoyuan decoction for relieving fatigue and regulating immunity, and the preparation method thereof comprises:

[0011] (1) Grind ginseng, astragalus, liquorice and cinnamon to obtain raw material A;

[0012] (2) mixing raw material A and extraction solvent 1, adding mixed enzyme, extracting, and filtering to obtain extract 1 and filter residue 1;

[0013] (3) Mixing the filter residue 1 and water to obtain a filter residue liquid, introducing ozone into the mixture while stirring, filtering, washing, and drying to obtain raw material B;

[0014] (4) mixing raw material B and extraction solvent 2, extracting, and combining the extracts to obtain extract 2;

[0015] (5) Evenly mix the extract 1 and the extract 2, centrifuge, take the supernatant, add water, and obtain the Baoyuan decoction for relieving fatigue and regulating immunity.

[0016] Preferably, in step (1), the weight ratio of ginseng, astragalus, liquorice and cinnamon is 5-7:10-15:2-5:0.5-2.

[0017] Further preferably, in step (1),

[0018] The weight ratio of ginseng, astragalus, licorice and cinnamon is 6:12:3:1.2.

[0019] More preferably, in step (1), the powder is sieved through a 60-mesh sieve after being crushed.

[0020] Preferably, in step (2), the mass ratio of the raw material A to the extraction solvent 1 is 1:5-8, more preferably 1:6.

[0021] Preferably, in step (2), the extraction solvent 1 is water.

[0022] Preferably, in step (2), the mixed enzyme is a mixture of pectinase, cellulase and xylanase, and further preferably, the mass ratio of pectinase, cellulase and xylanase is 0.5-2:1-5:0.5-2, and further preferably 1:2:1.

[0023] Preferably, in step (2), the extraction conditions are: extraction at 35-55°C for 20-60 min, more preferably extraction at 52°C for 40 min.

[0024] Preferably, in step (3), the solid content of the filtrate liquid is 10-30%, more preferably 20%.

[0025] Preferably, in step (3), the amount of ozone introduced is 0.3-1% of the mass of the filtrate liquid, and more preferably 0.7%.

[0026] Preferably, in step (3), the ozone treatment time is 10-30 min, more preferably 18 min.

[0027] Preferably, in step (3), the drying is oven drying, and further preferably, the temperature of the oven drying is not higher than 60°C.

[0028] Preferably, in step (4), the extraction solvent 2 is at least one of water, 60-80% ethanol, methanol, acetone, petroleum ether, and chloroform, and is further preferably a mixture of water, 60-80% ethanol and petroleum ether. Further, the volume ratio of the water, 60-80% ethanol and petroleum ether is 1:0.2-1:0.2-1, and is further preferably 1:0.5:0.3. Further, the water is purified water, and the ethanol is 75% ethanol.

[0029] Preferably, in step (4), the mass ratio of the solvent 2 to the raw material B is 1:5-10, more preferably 1:8.

[0030] Preferably, in step (4), the extraction is performed 2-5 times, more preferably 2 times.

[0031] Preferably, in step (4), the extraction conditions are: extraction at 40-60°C for 20-80 min, more preferably extraction at 50°C for 30 min.

[0032] Preferably, in step (5), the adding of water is adding purified water.

[0033] Preferably, in step (5), the adding of water is to adjust the crude drug concentration to 0.012-0.020 g / mL, more preferably 0.013 g / mL.

[0034] Preferably, the preparation method of the Baoyuan Decoction for relieving fatigue and regulating immunity further comprises the steps of filling and sterilizing.

[0035] Preferably, the filling is: 280 mL per bottle.

[0036] Preferably, the sterilization condition is: sterilization at 115-125° C. for 10-18 min, and most preferably sterilization at 121° C. for 15 min. The inventors found in production that too long a sterilization time would lead to a decrease in the content of ginsenosides.

[0037] In another aspect, the present invention provides the use of the Baoyuan Decoction in the preparation of medicines for treating and relieving fatigue and improving immunity.

[0038] The beneficial effects of the present invention include at least:

[0039] (1) The Baoyuan Decoction after the improved process of the present invention has improved bioavailability and good storage stability, and has good anti-fatigue effect and immunity-enhancing effect.

[0040] (2) By using ozone treatment, the internal structure of the filter residue is loosened, which promotes the further dissolution of the active ingredients, greatly increases the content of the active ingredients, and improves the efficacy.

[0041] (3) By screening mixed enzymes, it was found that mixed treatment with pectinase, cellulase and xylanase could achieve a greater degree of dissolution of the active ingredients.

[0042] (4) With respect to the formula composition of Baoyuan Decoction, the present invention also provides targeted extraction solvents and extraction conditions to improve the anti-fatigue and immunity-enhancing effects of Baoyuan Decoction, reduce the dosage of medication, and improve patient compliance. DETAILED DESCRIPTION

[0043] In order to make the technical means, creative features, purpose and efficacy of the present invention easy to understand, the present invention is further explained in conjunction with specific embodiments below, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the embodiments in the implementation mode, other embodiments obtained by those skilled in the art without creative work all belong to the protection scope of the present invention. In the following embodiments, unless otherwise specified, the operating methods used are conventional operating methods, the equipment used are conventional equipment, and the equipment materials used in each embodiment are the same.

[0044] In the following examples, some raw materials are from the following sources, which do not limit the present application:

[0045] Ginseng was produced in Jilin and purchased from Hangzhou Huadong Chinese Medicine Pieces Co., Ltd.

[0046] Astragalus was produced in Gansu and purchased from Hangzhou Huadong Chinese Medicine Pieces Co., Ltd.

[0047] Licorice was produced in Xinjiang Uygur Autonomous Region and purchased from Hangzhou Huadong Chinese Medicine Pieces Co., Ltd.

[0048] Cinnamon bark was produced in Guangxi Zhuang Autonomous Region and purchased from Hangzhou Huadong Chinese Medicine Pieces Co., Ltd.;

[0049] The pectinase was Novozymes Pectinex Ultra Clear, with an enzyme activity of 8600U / g;

[0050] The cellulase was Cosmay AL573708136185, with an enzyme activity of 10000 U / g;

[0051] The xylanase used was Huaxi 101, with an enzyme activity of 10000U / g;

[0052] Ginsenoside Rg1 standard was purchased from China National Institute for Food and Drug Control with a purity of 94.0%;

[0053] Ginsenoside Re standard was purchased from China National Institute for Food and Drug Control with a purity of 96.0%;

[0054] Ginsenoside Rb1 standard was purchased from China National Institute for Food and Drug Control with a purity of 94.3%.

[0055] Example 1

[0056] A Baoyuan decoction for relieving fatigue and regulating immunity, the preparation method of which is as follows:

[0057] (1) Mix 6 g of ginseng, 12 g of astragalus, 3 g of licorice, and 1.2 g of cinnamon, grind them, and pass through a 60-mesh sieve to obtain raw material A;

[0058] (2) Mix raw material A and 6 times the weight of purified water, heat to 52° C., add mixed enzyme (a mixture of pectinase, cellulase and xylanase, with a mass ratio of 1:2:1), keep at 52° C. and stir for extraction for 40 min. After the extraction is completed, heat to 80° C. to inactivate the enzyme for 3 min, filter, and rinse with purified water 5 times to obtain extract 1 and residue 1;

[0059] (3) Mix the filter residue 1 and purified water, adjust the solid content (mass fraction of the filter residue 1) to 20%, and obtain a filter residue liquid. Use an ozone generator to introduce ozone treatment while stirring. The amount of ozone introduced is 0.7% of the mass of the filter residue liquid. Stir for 18 minutes, filter, rinse with purified water 5 times, transfer to an oven, and dry at 55° C. to obtain raw material B;

[0060] (4) Mixing raw material B and 8 times the weight of extraction solvent 2 (the volume ratio of purified water, 75% ethanol and petroleum ether is 1:0.5:0.3), extracting at 50° C. for 30 min, repeating the extraction twice, and combining the extracts to obtain extract 2;

[0061] (5) The extract 1 obtained in step (2) and the extract 2 obtained in step (4) are mixed evenly, centrifuged at 8000 r / min for 3 min, the supernatant is collected, and purified water is added to adjust the crude drug concentration to 0.013 g / mL, thereby obtaining Baoyuan Decoction for relieving fatigue and regulating immunity.

[0062] Example 2

[0063] (1) Mix 6 g of ginseng, 12 g of astragalus, 3 g of licorice, and 1.2 g of cinnamon, grind them, and pass them through a 60-mesh sieve to obtain raw material A;

[0064] (2) Mix raw material A and 8 times the weight of purified water, heat to 55° C., add mixed enzyme (a mixture of pectinase, cellulase and xylanase, with a mass ratio of 0.5:5:0.5), keep at 55° C. and stir for extraction for 20 min. After the extraction is completed, heat to 80° C. to inactivate the enzyme for 3 min, filter, and rinse with purified water 5 times to obtain extract 1 and residue 1;

[0065] (3) Mix the filter residue 1 and purified water, adjust the solid content (mass fraction of the filter residue 1) to 10%, and obtain a filter residue liquid. Use an ozone generator to introduce ozone while stirring. The amount of ozone introduced is 1% of the mass of the filter residue liquid. Stir for 10 minutes, filter, rinse with purified water 5 times, transfer to an oven, and dry at 55° C. to obtain raw material B;

[0066] (4) Mixing raw material B and 10 times the weight of extraction solvent 2 (the volume ratio of purified water, 75% ethanol and petroleum ether is 1:0.2:0.2), extracting at 60° C. for 20 min, repeating the extraction twice, and combining the extracts to obtain extract 2;

[0067] (5) The extract 1 obtained in step (2) and the extract 2 obtained in step (4) are mixed evenly, centrifuged at 8000 r / min for 3 min, the supernatant is collected, and purified water is added to adjust the crude drug concentration to 0.013 g / mL, thereby obtaining Baoyuan Decoction for relieving fatigue and regulating immunity.

[0068] Example 3

[0069] (1) Mix 6 g of ginseng, 12 g of astragalus, 3 g of licorice, and 1.2 g of cinnamon, grind them, and pass them through a 60-mesh sieve to obtain raw material A;

[0070] (2) Mix raw material A and 8 times the weight of purified water, heat to 35° C., add mixed enzyme (a mixture of pectinase, cellulase and xylanase, with a mass ratio of 2:1:2), keep at 35° C. and stir for extraction for 60 min. After the extraction is completed, heat to 80° C. to inactivate the enzyme for 3 min, filter, and rinse with purified water 5 times to obtain extract 1 and residue 1;

[0071] (3) Mix the filter residue 1 and purified water, adjust the solid content (mass fraction of the filter residue 1) to 30%, and obtain a filter residue liquid. Use an ozone generator to introduce ozone while stirring. The amount of ozone introduced is 0.3% of the mass of the filter residue liquid. Stir for 30 minutes, filter, rinse with purified water 5 times, transfer to an oven, and dry at 55° C. to obtain raw material B;

[0072] (4) Mixing raw material B and 5 times the weight of extraction solvent 2 (purified water, 75% ethanol and petroleum ether in a volume ratio of 1:1:1), extracting at 40° C. for 80 min, repeating the extraction twice, and combining the extracts to obtain extract 2;

[0073] (5) The extract 1 obtained in step (2) and the extract 2 obtained in step (4) are mixed evenly, centrifuged at 8000 r / min for 3 min, the supernatant is collected, and purified water is added to adjust the crude drug concentration to 0.013 g / mL, thereby obtaining Baoyuan Decoction for relieving fatigue and regulating immunity.

[0074] Example 4

[0075] Different from Example 1, in step (3) of Example 4-13, the amount of ozone introduced is adjusted as follows, and the other conditions remain unchanged:

[0076]

[0077]

[0078] Examples 14-19

[0079] Different from Example 1, in step (2) of Examples 14-19, the composition of the mixed enzyme is adjusted as follows, and the other conditions remain unchanged:

[0080] Mass ratio of pectinase, cellulase and xylanase Embodiment 14 0:4:0 Embodiment 15 0:2:2 Example 16 2:2:0 Embodiment 17 0.5:3:0.5 Embodiment 18 1.5:1:1.5 Embodiment 19 2:0:2

[0081] Embodiment 20

[0082] The Baoyuan Decoction obtained in Example 1 was filled in a conventional manner, with 280 mL per bottle, and sterilized at 121° C. for 15 min to obtain the product.

[0083] Comparative Example 1

[0084] Different from Example 1, in step (4), the extraction solvent 2 is a mixture of purified water and 75% ethanol in a volume ratio of 1:0.5.

[0085] Comparative Example 2

[0086] The difference from Example 20 is that the sterilization condition is 116° C. for 40 min, and the rest is the same.

[0087] Results

[0088] 1. Ginsenoside content detection

[0089] Detection method: Refer to the 2020 "Chinese Pharmacopoeia" Volume 1, the method for determining the content of ginsenosides in ginseng.

[0090] Test solution: Centrifuge the product (12000 rpm, 10 min) and take the supernatant.

[0091] Reference solution: Accurately weigh ginsenoside Rg1 reference, ginsenoside Re reference and ginsenoside Rb1 reference, add methanol to make a mixed solution containing 0.2 mg of each per 1 ml, shake well, and the solution is ready. Chromatographic conditions:

[0092] Chromatographic column: Welch Ultimate AQ-C18 4.6*250mm 5μm

[0093] Flow rate: 1.0ml / min

[0094] Column temperature: 30℃

[0095] Detection wavelength: 203nm

[0096] Injection volume: 10 μL.

[0097] Use acetonitrile as mobile phase A and water as mobile phase B, and perform gradient elution according to the following table:

[0098] Test results:

[0099] The results of Examples 1-19 and Comparative Example 1 are as follows:

[0100]

[0101]

[0102] The results showed that ozone treatment greatly increased the extraction of insoluble matter from the filter residue. The change in ozone dosage showed that the extraction of effective substances first increased and then decreased. When the ozone intake was 0.7%, it was most conducive to the extraction of effective substances. In addition, the treatment of different mixed enzymes will also lead to different contents of effective ingredients in the first extraction, and will also affect the cell structure of the filter residue, thus affecting the progress of subsequent ozone treatment. When the mixed enzyme is a mixture of pectinase, cellulase and xylanase, and the mass ratio is 1:2:1, it is most conducive to the extraction of effective substances.

[0103] After testing according to the following method, the physical and chemical indicators of Example 20 and Comparative Example 2 all met the requirements:

[0104]

[0105] In addition, the results of ginsenoside content determination are as follows:

[0106]

[0107] The results showed that too long sterilization time had no significant effect on the properties, but would cause a significant decrease in the content of ginsenosides. A sterilization time of 15 minutes could retain the effective substances to the maximum extent.

[0108] 2. Stability test

[0109] Detection method: The sample of Example 20 was placed under accelerated conditions (40°C) to examine the changes in properties, clarity and content indicators after different periods of time.

[0110] Test results:

[0111]

[0112] The results showed that under accelerated conditions, the properties and smell of the sample in Example 20 were stable and unchanged within 30 days, and the content of the active ingredient was maintained at a good level, proving that the extract solution obtained by the extraction method of the present invention had good stability.

[0113] 3. Experiment on relieving fatigue and improving immunity

[0114] 3.1 Weighted swimming test

[0115] Male Kunming mice, weighing 18-22g, after the pre-swimming test, 60 mice with correct swimming posture were selected, raised in plastic cages, 12 / 12h light / dark cycle, free drinking water and diet, and the feeding conditions were 25°C and 40-50% air humidity, keeping the environment stable, and adaptive feeding for 1 week, weighed, and the mice were randomly divided into groups, 10 in each group. The experimental group was gavaged once a day, the dosage was 0.5g / kg, the gavage volume was 10mL / kg, and the drugs of Example 1, Example 2, Example 3, Example 4, and Example 5 were administered respectively, and the control group was gavaged with an equal amount of normal saline for one week. 30 minutes after the last administration, weighed, and the mouse weight-bearing swimming test was carried out, with a weight of 5% body weight lead, and the water temperature was 25°C. The swimming time of each group of mice was recorded (the time from the beginning of the mouse swimming to the exhaustion of sinking under the water surface for 8s and not being able to float to the surface), and the average value was calculated.

[0116] Mouse body weight measurement results:

[0117] Group Initial body weight (g) Last weight (g) Growth (g) Control group 20.6±0.92 34.2±1.35 13.6±1.09 Example 1 20.3±0.61 33.9±1.81 13.6±1.54 Example 2 19.7±0.52 33.4±0.94 13.7±1.17 Example 3 21.1±0.86 34.6±1.73 13.5±0.92 Example 4 19.8±0.43 33.0±1.65 13.2±1.47 Example 5 20.2±0.88 34.1±1.20 13.9±0.69

[0118] Note: Compared with the control group, *P<0.05.

[0119] The results showed that there was no statistical difference in body weight among the groups (p>0.05), indicating that the drug of the present invention had no significant effect on the body weight of mice and was highly safe.

[0120] Weighted swimming time results:

[0121] Group Swimming time Control group 23.8±5.31 Example 1 52.7±10.27** Example 2 45.1±13.64** Example 3 44.4±8.05** Example 4 35.6±10.33 Example 5 38.7±12.40*

[0122] Note: Compared with the control group, *P<0.05, **P<0.01.

[0123] The results showed that the weight-bearing swimming time of mice was significantly prolonged in the groups of Examples 1-3 and Example 5, among which Example 1 had the best effect, while the prolongation effect of Example 4 was not significant.

[0124] 3.2 Mononuclear macrophage carbon clearance experiment

[0125] Male Kunming mice, weighing 18-22g, were raised in plastic cages, with a 12 / 12h light / dark cycle, free access to water and diet, and the feeding conditions were a temperature of 25°C and an air humidity of 40-50%. The environment was kept stable. After one week of adaptive feeding, the mice were randomly divided into groups, with 10 mice in each group. The experimental group was gavaged once a day, with a dose of 0.5g / kg and a gavage volume of 10mL / kg. The drugs of Example 1, Example 2, Example 3, Example 4, and Example 5 were administered respectively, and the control group was gavaged with an equal amount of normal saline for 4 consecutive weeks. After the last administration, the body weight m1 was recorded, and the mice were injected with Indian ink (diluted with 4 times the volume of normal saline in advance) through the tail vein, with an injection volume of 10mL / k. The timing was immediately started after the ink was injected, and 20μL of blood was taken from the medial angular venous plexus with a capillary tube at 2 and 12 minutes after the injection, respectively, and a sodium carbonate solution with a mass fraction of 0.1% was added, mixed evenly, and a mixed solution with a volume concentration of 0.1% was configured to zero. A sodium carbonate solution with a mass fraction of 0.1% was used as a blank sample, and the optical density was measured at a wavelength of 600 nm.

[0126] After the determination, the mice were killed by cervical dislocation, and the liver and spleen were weighed, and the weight m2 was recorded to calculate the phagocytic index K, and then the phagocytic activity a and the organ index were calculated. The calculation formulas are as follows:

[0127] K = (1gOD1-1gOD2) / (t2-t1);

[0128] Among them, OD1 and OD2 are the optical densities of the two blood samplings, and t2-t1 is the time difference between the two blood samplings;

[0129] a=K 1 / 3 ×m1 / m2;

[0130] Organ index = spleen weight / body weight × 100%.

[0131] The results are as follows:

[0132] Group Organ index (%) Phagocytic activity Control group 0.351±0.019 6.83±0.59 Example 1 0.365±0.024 9.36±0.82** Example 2 0.362±0.013 8.70±0.46* Example 3 0.367±0.032 8.54±0.73* Example 4 0.349±0.016 7.62±0.69 Example 5 0.358±0.011 8.13±0.66*

[0133] Note: Compared with the control group, *P<0.05, **P<0.01.

[0134] The mononuclear macrophage system (RES) is an important component of the body's nonspecific immune system, which can quickly remove a variety of pathogenic substances and maintain the stability of the body's internal environment. The results showed that the groups of Examples 1-3 and Example 5 significantly improved the phagocytic activity of macrophages, among which Example 1 had the best effect, indicating that the extract of the present invention has a good effect of enhancing immunity.

[0135] In summary, the Baoyuan Decoction with improved process of the present invention increases the content of effective ingredients, thereby further improving the anti-fatigue effect and the immunity-enhancing effect. At the same time, the accelerated test results show that the product has good storage stability.

[0136] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A Baoyuan decoction for relieving fatigue and regulating immunity, characterized in that: The preparation method comprises the following steps: (1) Grind ginseng, astragalus, licorice and cinnamon to obtain raw material A; (2) Mixing raw material A and extraction solvent 1, adding mixed enzyme, extracting, filtering to obtain extract 1 and filter residue 1; the mixed enzyme is a mixture of pectinase, cellulase and xylanase, and the mass ratio of pectinase, cellulase and xylanase is 0.5-2:1-5:0.5-2; the mass ratio of raw material A and extraction solvent 1 is 1:5-8, the extraction solvent 1 is water, and the extraction conditions are: extraction at 35-55°C for 20-60 min; (3) Mix the filter residue 1 and water to obtain a filter residue liquid, introduce ozone into the mixture while stirring, filter, wash, and dry to obtain raw material B; the solid content of the filter residue liquid is 10-30%, the amount of ozone introduced is 0.5-1% of the mass of the filter residue liquid, and the ozone treatment time is 10-30 minutes; (4) Mixing raw material B and extraction solvent 2, extracting, and combining the extracts to obtain extract 2; the extraction solvent 2 is a mixture of water, 60-80% ethanol and petroleum ether, the mass ratio of the extraction solvent 2 to raw material B is 1:5-10, the number of extractions is 2-5 times, and the extraction conditions are: extraction at 40-60° C. for 20-80 min; (5) Evenly mix the extract 1 and the extract 2, centrifuge, take the supernatant, add water, and obtain the Baoyuan decoction for relieving fatigue and regulating immunity.

2. The Baoyuan Decoction for relieving fatigue and regulating immunity according to claim 1, characterized in that: The mass ratio of the pectinase, the cellulase and the xylanase is 1:2:

1.

3. The Baoyuan decoction for relieving fatigue and regulating immunity according to claim 1, characterized in that: The solid content of the filtrate liquid was 20%, the amount of ozone introduced was 0.7% of the mass of the filtrate liquid, and the ozone treatment time was 18 minutes.

4. The Baoyuan Decoction for relieving fatigue and regulating immunity according to claim 1, characterized in that: In step (4), the volume ratio of water, 60-80% ethanol and petroleum ether is 1:0.5:0.3, the mass ratio of solvent 2 and raw material B is 1:8, the number of extractions is 2 times, and the extraction conditions are: extraction at 50° C. for 30 min.

5. The Baoyuan Decoction for relieving fatigue and regulating immunity according to claim 1, characterized in that: The preparation method of the Baoyuan Decoction for relieving fatigue and regulating immunity also includes the steps of filling and sterilization. The sterilization conditions are: sterilization at 115-125° C. for 10-18 minutes.

6. Use of the Baoyuan decoction according to any one of claims 1 to 5 in the preparation of medicines for treating and relieving fatigue and improving immunity.

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