A method and device for preparing immobilized acetylcholine filler

Abstract

The present invention relates to the field of biomedical technology, and in particular to a preparation method and equipment for an immobilized acetylcholine filler. The epoxy resin is cleaned and impurities are removed for standby use. The cleaning solvent is ultrapure water. The impurities are removed by gauze elution. After cleaning and impurities are removed, the resin is dried. The epoxy resin is weighed and a buffer solution is added and stirred evenly. After stirring, the buffer solution is filtered out and dried. The buffer solution is added again to complete ring-opening activation to obtain an epoxy resin mixture containing an opened epoxy ring. Acetylcholine is added to the mixture and mixed evenly to obtain an acetylcholine filler blank. Water in the acetylcholine filler blank is drained to obtain the acetylcholine filler. Acetylcholine is fixed on the resin filler and the activity of cholinesterase in the blood is reduced by blood perfusion to reduce the aggravation of the neurotoxicity of Alzheimer's patients.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technology, and in particular to a preparation method and equipment for an immobilized acetylcholine filler. Background Art

[0002] Acetylcholine (ACh) is the first neurotransmitter discovered by humans. The neurotransmission mediated by acetylcholine is the basis of nervous system function. Sudden blockage of ACh-mediated neurotransmission is fatal. Its gradual loss is associated with the gradual deterioration of cognition, autonomic nerves and neuromuscular function, leading to diseases such as Alzheimer's disease and multiple system atrophy. Acetylcholinesterase (AChE) is an efficient hydrolase that catalyzes the hydrolysis of acetylcholine to produce choline and acetate ions. Acetylcholinesterase is a key enzyme in biological neurotransmission. At cholinergic synapses, the enzyme can degrade acetylcholine, terminate the excitatory effect of neurotransmitters on the postsynaptic membrane, and ensure the normal transmission of nerve signals in the body.

[0003] Accumulation of acetylcholinesterase may interact with beta-amyloid plaques and cause increased neurotoxicity. Beta-amyloid plaques are extracellular protein deposits that are a major hallmark of Alzheimer's disease.

[0004] Therefore, the present invention proposes an immobilized acetylcholine filler, which can greatly reduce the accumulation of acetylcholinesterase and reduce the neurotoxicity caused by the interaction with β-amyloid protein plaques by fixing exogenous acetylcholine and combining and reacting with acetylcholinesterase in the body. Summary of the invention

[0005] The purpose of the present invention is to provide a method and equipment for preparing an immobilized acetylcholine filler to solve the problems raised in the above background technology.

[0006] A method for preparing an immobilized acetylcholine filler comprises weighing raw materials in parts by mass, wherein the raw materials include epoxy resin and acetylcholine, and comprising the following steps:

[0007] 1) The epoxy resin is cleaned and removed for use. The cleaning solvent is ultrapure water. The impurity removal method is gauze leaching. After cleaning and removing impurities, the resin is dried. After drying, the humidity of the resin is maintained at 50% to 70%;

[0008] 2) Weigh 1-5 parts (mass) of epoxy resin and add 4-8 parts (volume) of buffer solution and stir evenly for 2-4 hours. After stirring, filter out the buffer solution and spin dry. The humidity of the resin after spinning is maintained at 50%-70%;

[0009] 3) Add 1 to 5 parts (volume) of buffer solution again, and shake on a shaker for 3 to 5 hours to complete the ring-opening activation to obtain an epoxy resin mixture containing an opened epoxy ring;

[0010] 4) Add 1 to 5 parts (by weight) of acetylcholine to the mixture in step 3 and mix evenly for 4 to 6 hours to obtain an acetylcholine filler blank;

[0011] 5) Drain the water in the acetylcholine filler blank, control the humidity of the acetylcholine filler to be maintained at 70% to 90%, and obtain the acetylcholine filler.

[0012] In step 1, at least 5 cleanings are required. In steps 2 and 3, the buffer is Tris buffer, and the pH value of Tris buffer is 7-9; the stirring rate is 90-110 ml / min; and the shaking frequency of the shaker is 80-120 r / min.

[0013] A device for preparing immobilized acetylcholine filler comprises a bracket, wherein the bracket is provided with a cleaning device, a drying device and a weighing device in sequence from top to bottom, wherein the cleaning device comprises a bin body, wherein a spray assembly and a filter assembly are provided in the bin body, wherein the spray assembly is located above the filter assembly, and wherein a circulation device is further provided on the bin body, wherein the circulation device is used to deliver the resin below the filter assembly to above the filter assembly.

[0014] By adopting the above technical scheme: the cleaning device is provided with a spray component and a filter component for cleaning and removing impurities from the resin, the drying device is used to dehydrate and dry the resin, the weighing device is used to weigh the dried resin, and the circulation device is used to repeatedly clean the resin in the cleaning device.

[0015] The filter assembly includes multiple filter units, each filter unit includes a first filter frame and a second filter frame arranged up and down, a filter slot is opened in the middle of the first filter frame, and guide blocks are arranged on both sides of the filter slot; a guide block is arranged in the middle of the second filter frame, and filter slots are arranged on both sides of the guide block; gauze is arranged in the filter slots of the first filter frame and the second filter frame, the width of the guide block on the second filter frame is greater than the width of the filter slot on the first filter frame; the height of the cross-sectional line of the guide block on the first filter frame decreases from both ends to the middle, and the height of the cross-sectional line of the guide block on the second filter frame decreases from the middle to both ends, and magnetic materials are arranged in the guide blocks.

[0016] By adopting the above technical solution: considering the need to remove iron filings in the resin, two first filter frames and second filter frames are provided in the filter unit for use in conjunction with each other, the resin flows through the guide block on the first filter frame, the guide block sends the resin to the filter slot of the first filter frame for filtration through the gauze, the resin continues to fall from the first filter frame onto the guide block of the second filter frame, and is then sent by the guide block to the filter slot of the second filter frame for secondary filtration through the gauze, and by setting up multiple filter units, it is beneficial to make the resin fully contact with the guide block, so that the iron filings in the resin can be well removed through the magnetic material inside the guide block.

[0017] A slot is provided in the guide block parallel to the filter tank, a magnet is provided in the slot, an opening communicating with the slot is provided on one side of the bin body, and a baffle for closing the opening is connected to one end of the magnet.

[0018] By adopting the above technical solution: the slot on the guide block is used to insert the magnet, and the magnet can be taken out from the opening outside the bin body by opening the baffle. When the magnet is taken out, the guide blocks on all filter racks lose their magnetic cores, and the surface of the guide block can be effectively cleaned by flushing.

[0019] The cross-sectional line of the guide block is an arc line.

[0020] By adopting the above technical solution: the cross-sectional line of the guide block is an arc line, which means that the upper surface of the guide block is an arc surface. When the resin flows through the guide block, it has a larger contact area with the guide block, which is beneficial to improving the removal effect of iron filings in the resin.

[0021] The spray assembly includes a spray head, a bin cover is provided at the top of the bin body, a feed port is provided at the top of the bin cover, the spray head is arranged on the inner wall of the bin cover, and the height of the cross-section line of the inner wall of the bin cover decreases from the middle to both sides.

[0022] By adopting the above technical solution: the internal components of the bin body can be inspected by opening the bin cover, the spray head is arranged on the bin cover, and the cross-sectional line of the inner wall of the bin cover is conducive to improving the use effect of the spray head. When the spray head is blocked, the resin can be cleaned further by removing the bin cover.

[0023] The circulation device includes a feed barrel, a discharge pipe is provided at the top of the feed barrel, a feed pipe is provided at the bottom of the feed barrel, a conveying screw is installed in the feed barrel, a first material valve is installed at the bottom of the bin body, a first pipe opening connected to the feed pipe is opened on the side wall of the discharge port at the bottom of the bin body, the first pipe opening is located above the first material valve, and a second pipe opening connected to the discharge pipe is opened on the top side wall of the bin body above the filter assembly.

[0024] By adopting the above technical solution: when the cleaning process needs to be repeated for multiple times, close the first material valve, then start the conveying screw inside the feed barrel to start working, and the conveying screw will transport the resin that falls on the bottom of the bin body to the discharge pipe again through the feed pipe. At this time, it will be vertically filtered through the filter assembly again, and this process will be repeated until the cleaning times are completed.

[0025] The spin-drying device includes a spin-drying barrel with an outer shell and a mesh cover structure, the bottom of the spin-drying barrel has a funnel-shaped neck, the neck is installed on the bottom end surface of the outer shell through a rotating support seat, a pulley groove is provided on the outer side of the neck, a second material valve is installed in the neck, a transmission shaft is installed on the bracket, the upper end of the transmission shaft is connected to the rotating shaft of the conveying screw, and the lower end of the transmission shaft is installed with a pulley, and the pulley is connected to the pulley groove through a belt drive.

[0026] By adopting the above technical solution: opening the first material valve, the resin cleaned by the cleaning device falls into the spin-drying barrel, the power of the rotating shaft on the conveying screw is transmitted to the neck of the spin-drying barrel through the transmission shaft, the rotating support seat provides support for the spin-drying barrel, and the dried resin falls from the neck after humidity detection.

[0027] The weighing device includes a funnel-shaped weighing tray, which is located below the neck. A third valve is installed on the discharge port of the weighing tray. Support blocks are fixedly connected to the two side edges of the weighing tray. A cross bar is provided below the bracket corresponding to the support block, and a weighing module is connected between the support block and the cross bar.

[0028] By adopting the above technical solution: the weighing tray weighs the resin through the weighing module, and the third material valve is opened, so that the weighed resin can be taken out from the discharge port of the weighing tray.

[0029] In summary, the present invention has the following beneficial effects:

[0030] In the present invention, acetylcholine is fixed on a resin filler, and the activity of cholinesterase in the blood is reduced by blood perfusion, so as to reduce the aggravation of the condition of Alzheimer's patients caused by neurotoxicity.

[0031] In the present invention, the cleaning device is provided with a spray assembly and a filter assembly for cleaning and removing impurities from the resin, a drying device for dehydrating and drying the resin, a weighing device for weighing the dried resin, and a circulation device for repeatedly cleaning the resin in the cleaning device.

[0032] In the present invention, considering the need to remove iron filings in the resin, a first filter frame and a second filter frame that are used in conjunction with each other are provided in the filter unit. The resin flows through the guide block on the first filter frame, and the guide block sends the resin to the filter slot of the first filter frame for filtration through a gauze. The resin continues to fall from the first filter frame onto the guide block of the second filter frame, and is then sent by the guide block to the filter slot of the second filter frame for secondary filtration through a gauze. By setting up multiple filter units, it is beneficial to make the resin fully contact with the guide block, so that the iron filings in the resin can be well removed through the magnetic material inside the guide block. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0034] Figure 1 A schematic diagram of the structure of the device for preparing the immobilized acetylcholine filler provided by the present invention;

[0035] Figure 2 A schematic cross-sectional view of a cleaning device for a device for preparing an immobilized acetylcholine filler provided by the present invention;

[0036] Figure 3 A schematic side structural view of a cleaning device for an apparatus for preparing an immobilized acetylcholine filler provided by the present invention;

[0037] Figure 4 A schematic cross-sectional structural diagram of a drying device of an apparatus for preparing an immobilized acetylcholine filler provided by the present invention;

[0038] Figure 5 A schematic cross-sectional structural diagram of a weighing device of an apparatus for preparing an immobilized acetylcholine filler provided by the present invention;

[0039] Figure 6 A schematic structural diagram of a first filter frame of the device for preparing immobilized acetylcholine filler provided by the present invention;

[0040] Figure 7 A schematic structural diagram of a second filter frame of the device for preparing immobilized acetylcholine filler provided by the present invention;

[0041] Figure 8 is a comparative schematic diagram of the first filter rack and the second filter rack;

[0042] Figure numerals: 1. bracket; 2. bin body; 3. bin cover; 4. feed barrel; 5. outer shell; 6. weighing tray; 7. cross bar; 8. weighing module; 9. first filter frame; 10. second filter frame; 11. guide block; 12. gauze; 13. conveying screw; 14. feed pipe; 15. discharge pipe; 16. baffle; 17. belt; 18. rotating support seat; 19. spin dryer; 20. neck; 21. magnet; 22. pulley; 23. support block; 24. transmission shaft. DETAILED DESCRIPTION

[0043] The following examples illustrate the present invention in detail. In these examples, unless otherwise specified, all parts and percentages are calculated by weight.

[0044] The preparation method of the immobilized acetylcholine filler comprises weighing raw materials according to mass parts, and the raw materials include epoxy resin and acetylcholine.

[0045] Embodiment 1: The preparation method of the immobilized acetylcholine filler of this embodiment comprises the following steps:

[0046] 1) The epoxy resin is cleaned and removed for use. The cleaning solvent is ultrapure water. The impurity removal method is gauze rinsing. After cleaning and removing impurities, the resin is dried. After drying, the humidity of the resin is maintained at 50%;

[0047] 2) Weigh 1 part (mass) of epoxy resin and add 4 parts (volume) of buffer solution and stir evenly for 2 hours. After stirring, filter out the buffer solution and spin dry. The humidity of the resin after spinning is maintained at 50%;

[0048] 3) Add 1 part (volume) of buffer solution again and shake on a shaker for 3 hours to complete the ring-opening activation to obtain an epoxy resin mixture containing an opened epoxy ring;

[0049] 4) Add 1 part (by mass) of acetylcholine to the mixture in step 3 and mix evenly for 4 hours to obtain an acetylcholine filler blank;

[0050] 5) Drain the water in the acetylcholine filler blank and control the humidity of the acetylcholine filler to be maintained at 70% to obtain the acetylcholine filler.

[0051] Embodiment 2: The preparation method of the immobilized acetylcholine filler of this embodiment comprises the following steps:

[0052] 1) The epoxy resin is cleaned and removed for use. The cleaning solvent is ultrapure water. The impurity removal method is gauze rinsing. After cleaning and removing impurities, the resin is dried. After drying, the humidity of the resin is maintained at 60%;

[0053] 2) Weigh 2 parts (mass) of epoxy resin and add 6 parts (volume) of buffer solution and stir evenly for 3 hours. After stirring, filter out the buffer solution and spin dry. The humidity of the resin after drying is maintained at 60%;

[0054] 3) Add 2 parts (volume) of buffer again and shake on a shaker for 4 hours to complete the ring-opening activation to obtain an epoxy resin mixture containing an opened epoxy ring;

[0055] 4) Add 2 parts (by mass) of acetylcholine to the mixture in step 3 and mix evenly for 5 hours to obtain an acetylcholine filler blank;

[0056] 5) Drain the water in the acetylcholine filler blank, control the humidity of the acetylcholine filler to be maintained at 80%, and obtain the acetylcholine filler.

[0057] Embodiment 3: The preparation method of the immobilized acetylcholine filler of this embodiment comprises the following steps:

[0058] 1) The epoxy resin is cleaned and removed for use. The cleaning solvent is ultrapure water. The impurity removal method is gauze leaching. After cleaning and removing impurities, the resin is dried. After drying, the humidity of the resin is maintained at 70%;

[0059] 2) Weigh 5 parts (mass) of epoxy resin and add 8 parts (volume) of buffer solution and stir evenly for 4 hours. After stirring, filter out the buffer solution and spin dry. The humidity of the resin after drying is maintained at 70%;

[0060] 3) Add 5 parts (volume) of buffer again and shake on a shaker for 5 hours to complete the ring-opening activation to obtain an epoxy resin mixture containing an opened epoxy ring;

[0061] 4) Add 5 parts (by weight) of acetylcholine to the mixture in step 3 and mix evenly for 6 hours to obtain an acetylcholine filler blank;

[0062] 5) Drain the water in the acetylcholine filler blank, control the humidity of the acetylcholine filler to be maintained at 90%, and obtain the acetylcholine filler.

[0063] In the above-mentioned Examples 1 to 3, the number of washing times in step 1 is 5 times, the buffer in steps 2 and 3 is Tris buffer, and the pH value of Tris buffer is 8; secondly, the stirring rate is 100 ml / min; and the shaking frequency of the shaker is 100 r / min.

[0064] Experimental example

[0065] The same mass of acetylcholine fillers in Examples 1 to 3 were taken respectively, and the content of acetylcholine in the fixing solution was measured. The fixed acetylcholine was used to filter the choline acylesterase in the blood, and the number of choline acylesterase units was measured. The experimental results are shown in Tables 1-3 below.

[0066] Table 1 Experimental results of Example 1

[0067] Perfusion time The unit of cholinesterase is U / L Drop rate initial 13573 none 10min 12364 8.91% 15min 11295 16.78% 20min 8884 34.55% 30min 6022 55.63%

[0068] Table 2 Experimental results of Example 2

[0069] Perfusion time The unit of cholinesterase is U / L Drop rate initial 13505 none 10min 12033 10.91% 15min 10018 25.82% 20min 7543 44.14% 30min 5142 61.92%

[0070] Table 3 Experimental results of Example 3

[0071] Perfusion time The unit of cholinesterase is U / L Drop rate initial 13260 none 10min 11892 10.32% 15min 9978 24.75% 20min 7904 40.39% 30min 5541 58.21%

[0072] As can be seen from Tables 1-3 above, by adopting the preparation method of Example 2, acetylcholine is fixed on the resin filler, and then blood perfusion is performed, the activity of cholinesterase in the blood can be effectively reduced, thereby reducing the aggravation of the neurotoxicity to the condition of Alzheimer's patients.

[0073] Embodiment 4: The present invention also provides a device for preparing immobilized acetylcholine filler, such as Figure 1 As shown, it includes a bracket 1, and the bracket 1 is provided with a cleaning device, a drying device and a weighing device in sequence from top to bottom. The cleaning device includes a bin body 2, and a spray component and a filter component are provided in the bin body 2. The spray component is located above the filter component. A circulation device is also provided on the bin body 2, and the circulation device is used to send the resin below the filter component to the top of the filter component. The cleaning device is provided with a spray component and a filter component for cleaning and removing impurities from the resin. The drying device is used to dehydrate and dry the resin. The weighing device is used to weigh the dried resin, and the circulation device is used to repeatedly clean the resin in the cleaning device.

[0074] like Figure 2 As shown, the filter assembly includes a plurality of filter units, each filter unit includes a first filter frame 9 and a second filter frame 10 arranged up and down, a filter slot is provided in the middle of the first filter frame 9, and guide blocks 11 are provided on both sides of the filter slot; a guide block 11 is provided in the middle of the second filter frame 10, and filter slots are provided on both sides of the guide block 11; a gauze 12 is provided in the filter slots of the first filter frame 9 and the second filter frame 10, and Figure 8As shown, the width of the guide block 11 on the second filter frame 10 is greater than the width of the filter slot on the first filter frame 9; the height of the cross-sectional line of the guide block 11 on the first filter frame 9 decreases from both ends to the middle, and the height of the cross-sectional line of the guide block 11 on the second filter frame 10 decreases from the middle to both ends. The guide blocks 11 are both provided with magnetic materials. Considering the need to remove iron filings in the resin, two first filter frames 9 and second filter frames 10 are provided in the filter unit for use together. The resin flows through the guide block 11 on the first filter frame 9, and the guide block 11 sends the resin to the filter slot of the first filter frame 9 for filtration through the gauze 12. The resin continues to fall from the first filter frame 9 onto the guide block 11 of the second filter frame 10, and is then sent by the guide block 11 to the filter slot of the second filter frame 10 for secondary filtration through the gauze 12. By setting up multiple filter units, it is beneficial to make the resin fully contact with the guide block 11, so that the iron filings in the resin can be well removed by the magnetic material inside the guide block 11.

[0075] like Figure 3 As shown, a slot is provided in the guide block 11 parallel to the filter tank, a magnet 21 is provided in the slot, an opening connected to the slot is provided on one side of the bin body 2, one end of the magnet 21 is connected to a baffle 16 for closing the opening, the slot on the guide block 11 is used to insert the magnet 21, outside the bin body 2, the magnet 21 can be taken out from the opening by opening the baffle 16, when the magnet 21 is taken out, all the guide blocks 11 on the filter rack lose their magnetic cores, at this time, the screen 12 can be removed and the surface of the guide block 11 can be cleaned by flushing.

[0076] like Figure 6 and 7 As shown, the cross-sectional line of the guide block 11 is an arc line. The cross-sectional line of the guide block 11 being an arc line means that the upper surface of the guide block 11 is an arc surface. When the resin flows through the guide block 11, it has a larger contact area with the guide block 11, which is beneficial to improving the removal effect of iron filings in the resin.

[0077] Furthermore, the spray assembly includes a spray head, a bin cover 3 is provided at the top of the bin body 2, a feed port is provided at the top of the bin cover 3, the spray head is provided on the inner wall of the bin cover 3, the height of the cross-sectional line of the inner wall of the bin cover 3 decreases from the middle to both sides, the internal components of the bin body 2 can be inspected by opening the bin cover 3, the spray head is provided on the bin cover 3, and the cross-sectional line of the inner wall of the bin cover 3 is conducive to improving the use effect of the spray head, and when the spray head is blocked, the resin can continue to be cleaned by removing the bin cover 3.

[0078] The circulation device includes a feed barrel 4, a discharge pipe 15 is provided at the top of the feed barrel 4, a feed pipe 14 is provided at the bottom of the feed barrel 4, a conveying screw 13 is installed in the feed barrel 4, a first material valve is installed at the bottom of the bin body 2, a first pipe opening connected to the feed pipe 14 is opened on the side wall of the discharge port at the bottom of the bin body 2, the first pipe opening is located above the first material valve, and a second pipe opening connected to the discharge pipe 15 is opened on the top side wall of the bin body 2 above the filter assembly. When the cleaning process needs to be repeated for multiple times, the first material valve is closed, and then the conveying screw 13 inside the feed barrel 4 is started to work. The conveying screw 13 transports the resin that falls on the bottom of the bin body 2 to the discharge pipe 15 again through the feed pipe 14. At this time, it is vertically filtered through the filter assembly again, and this process is repeated until the cleaning times are completed.

[0079] like Figure 4 As shown, the spin drying device includes a shell 5 and a spin drying barrel 19 with a mesh cover structure, the bottom of the spin drying barrel 19 has a funnel-shaped neck 20, the neck 20 is installed on the bottom end surface of the shell 5 through a rotating support seat, the outer side of the neck 20 is provided with a pulley 22 groove, the neck 20 is installed with a second material valve, the bracket 1 is installed with a transmission shaft 24, the upper end of the transmission shaft 24 is connected to the rotating shaft of the conveying screw 13, the lower end of the transmission shaft 24 is installed with a pulley 22, the pulley 22 and the pulley 22 groove are connected by a belt 17, the first material valve is opened, and the cleaning device is The cleaned resin falls into the spin-drying barrel 19, and the power of the rotating shaft on the conveying screw 13 is transmitted to the neck 20 of the spin-drying barrel 19 through the transmission shaft 24. The rotating support seat provides support for the spin-drying barrel 19. The dried resin falls from the neck 20 after humidity detection. The rotating support seat in this embodiment can adopt a high-speed rotating bearing seat sealing structure of the Chinese invention patent with publication number CN117570116A. The sealing structure in this application is applied between the neck 20 and the bottom end surface of the outer shell 5, which can realize the high-speed rotation of the spin-drying barrel 19 while ensuring the sealing effect.

[0080] like Figure 5 As shown, the weighing device includes a funnel-shaped weighing tray 6, and the weighing tray 6 is located below the neck 20. A third valve is installed on the discharge port of the weighing tray 6. Support blocks 23 are fixedly connected to the two side edges of the weighing tray 6. A cross bar 7 is provided below the bracket 1 corresponding to the support block 23. A weighing module 8 is connected between the support block 23 and the cross bar 7. The weighing module 8 uses a weight sensor. The resin is weighed by the weighing module 8. The third valve is opened, and the weighed resin can be taken out from the discharge port of the weighing tray 6.

[0081] Finally, it should be noted that the above are only preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing an immobilized acetylcholine filler, characterized in that: Weigh the raw materials by mass, the raw materials including epoxy resin and acetylcholine, and include the following steps: 1) The epoxy resin is cleaned and removed for use. The cleaning solvent is ultrapure water. The impurity removal method is gauze leaching. After cleaning and removing impurities, the resin is dried. After drying, the humidity of the resin is maintained at 50% to 70%; 2) Weigh 1-5 parts (mass) of epoxy resin and add 4-8 parts (volume) of buffer solution and stir evenly for 2-4 hours. After stirring, filter out the buffer solution and spin dry. The humidity of the resin after spinning is maintained at 50%-70%; 3) Add 1 to 5 parts (volume) of buffer solution again, and shake on a shaker for 3 to 5 hours to complete the ring-opening activation to obtain an epoxy resin mixture containing an opened epoxy ring; 4) Add 1 to 5 parts (by weight) of acetylcholine to the mixture in step 3 and mix evenly for 4 to 6 hours to obtain an acetylcholine filler blank; 5) Drain the water in the acetylcholine filler blank, control the humidity of the acetylcholine filler to be maintained at 70% to 90%, and obtain the acetylcholine filler; The number of washing times in step 1 is 5 times, the buffer in steps 2 and 3 is Tris buffer, and the pH value of Tris buffer is 8; secondly, the stirring rate is 100 ml / min; and the shaking frequency of the shaker is 100 r / min.

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