A fermented agarwood preparation for broiler feeding and preparation method thereof

Abstract

The present invention belongs to the field of biological feed preparation, and in particular to a fermented agarwood preparation for broilers and a preparation method thereof. The ultrasonically extracted extract solution is modified under acidic conditions and then mixed with a starch mixture. The obtained hygroscopic basic nutritional powder contains all the nutrients required for broiler growth, and cooperates with creatine hydrate, fenugreek saponin, and L-alanyl-L-glutamine to improve the tenderness of broiler meat during the market period; the drug active solution is mixed with an active anthelmintic to protect the gastrointestinal mucosa of broilers, reduce lung damage in broilers, and kill parasites; the flavor-correcting fermentation product containing agarwood leaves obtained by fermentation increases the surface area after being puffed and modified by an expander, improves the hygroscopic capacity of the modified nutrient puffed powder, cooperates with sodium dehydroacetate and schisandra chinensis B, and the fermented agarwood preparation for broilers prepared is kept dry, does not produce mold, and each component is stably present.

Description

Technical Field

[0001] The invention belongs to the field of biological feed preparation, and specifically refers to a fermented feeding type agarwood preparation for broilers and a preparation method thereof. Background Art

[0002] Feed is the main source of energy and nutrition for broilers. For broiler farming, different feeds have a great impact on their growth and meat quality. High-protein, high-energy feeds can promote the growth of broilers, while high-fat, low-protein feeds will slow down the growth rate of broilers. Nutritional imbalance in feeds can also lead to broiler development, slow growth, and poor meat quality.

[0003] In addition to affecting the growth rate of broilers, feed also directly affects the quality of broiler meat. When fed with different feeds, there will be differences in the muscle tissue, fat distribution, meat tenderness, and water content of broilers. Pollution and toxins in the feed are also an important factor affecting the quality of broiler meat. If the feed contains too many impurities and pollutants, it will lead to excessive accumulation of toxins in the broiler body, which will affect the quality of the meat.

[0004] At present, the existing feeding preparation technology for broilers has the following problems: First, it cannot improve the meat quality of broilers at the market stage. As the age increases, the connective tissue with poor meat quality increases in the broilers, the muscle fibers become harder, the muscle water content decreases, and the meat is coarse; second, it has no disease resistance. The ammonia smell, hydrogen sulfide, and carbon dioxide concentrations inside the chicken house are high, which can easily cause respiratory diseases, lung infections, and lung damage. Broilers will also have parasites in their bodies during the feeding process; third, in hot and humid weather, chicken feed is put into the barrel and left for a long time, and the chicken feed at the bottom of the barrel will become moldy and produce toxins. Summary of the invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a fermented feeding agarwood preparation for broilers and a preparation method thereof. In order to solve the problems that the existing feeding preparations for broilers cannot improve the meat quality of broilers in the market period, cannot prevent and control lung damage and the occurrence of parasites, and are prone to mildew, the present invention uses agarwood leaves powder and a flavor-correcting fermentation product for expansion modification, and then mixes an anti-disease complex, creatine water, sodium dehydroacetate, fenugreek saponin, L-alanyl-L-glutamine, and schisandrin B. The fermented feeding agarwood preparation for broilers improves the meat quality of broilers in the market period, has disease resistance, and does not mildew.

[0006] In order to achieve the above-mentioned object, the technical solution adopted by the present invention is as follows: the present invention proposes a fermented agarwood preparation for broiler chickens, which specifically comprises the following components in parts by weight:

[0007] 100-120 parts of agarwood leaves, 200-240 parts of hygroscopic basic nutrient powder, 70-80 parts of disease-resistant compound, 60-80 parts of flavor-correcting fermentation bacteria agent, 35-50 parts of creatine hydrate, 15-20 parts of sodium dehydroacetate, 12-18 parts of fenugreek saponin, 15-20 parts of L-alanyl-L-glutamine and 15-20 parts of schisandra chinensis B.

[0008] Preferably, the flavor-correcting fermentation bacteria agent specifically includes: Corynebacterium glutamicum, Acinetobacter calcoaceticus, Enterobacter mulberry, and Tetradecanobacterium dodecanedi, and the mixing ratio by weight is 1-1.5:1-2:2-3:1.

[0009] Furthermore, the Corynebacterium glutamicum was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.15647; the Acinetobacter calcoaceticus was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.6186; the Enterobacter mulberryi was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.10322; and the Tetradecapsula dodecagrami was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.10746.

[0010] Preferably, the method for preparing the hygroscopic basic nutritional powder specifically comprises the following steps:

[0011] S1, weighing weight portions is 200-230 parts of Polygonum sibiricum, 120-150 parts of Agar grass, 150-180 parts of brown rice, putting into a dryer with a power of 1.3-1.8 kW, the drying temperature is 65-70 ° C, and the drying time is 1-1.5 h. After drying, a powder beating machine with a power of 1.6-2.2 kW is used, and the powder beating temperature is 25-28 ° C, and the powder beating time is 20-25 min. Powder is beaten to obtain a starch mixture;

[0012] S2, weighing 60-80 parts of Yunnan sorrel, 50-70 parts of Artemisia selengensis, and 40-50 parts of Brasenia schrenkiana by weight, and putting them into a vacuum freeze dryer with a power of 5.5-7.5 kW, with a vacuum degree of 60-80 Pa, a freezing temperature of -20°C, and a freezing time of 2-3 h, then heating to 2-4°C, drying for 1.2-1.5 h, and using a powdering machine with a power of 1.6-2.2 kW, a powdering temperature of 25-28°C, and a powdering time of 20-25 min to obtain a nutritional supplement mixed powder;

[0013] S3, weighing 220-280 parts by weight of ultrapure water and the mixed powder of the nutritional supplement prepared in S2, putting them into an ultrasonic extractor with a power of 1.2-1.5 kW, an ultrasonic frequency of 20-30 MHz, an ultrasonic extraction temperature of 40-45° C., and an ultrasonic extraction time of 8-12 min, and performing ultrasonic extraction to obtain an extract solution;

[0014] S4, weighing 60-70 parts of cecropin, 30-50 parts of octaarginine, and 120-130 parts of ultrapure water, putting them into a stirring tank with a power of 1.2-1.5 kW, stirring at a temperature of 23-25°C, stirring for 10-15 min, stirring at a speed of 110-120 r / min, stirring and mixing, adjusting the pH to 6.2 with a 2% hydrochloric acid solution, and mixing with the extract solution prepared in S3 to obtain a modified extract solution;

[0015] S5. Put the starch mixture prepared by S1 into the modified extract solution prepared by S4, stir at a temperature of 25-28°C, stir for 12-16 min, stir at a speed of 120-130 r / min, stir evenly, put into a dryer with a power of 1.3-1.8 kW, dry at a temperature of 35-42°C, dry for 1.2-1.6 h, and obtain hygroscopic basic nutrient powder after drying.

[0016] Preferably, the method for preparing the anti-disease compound specifically comprises the following steps:

[0017] L1. Weigh 35-40 parts by weight of sulforaphane, 30-40 parts by weight of cabbage sulforaphane, 30-40 parts by weight of citric acid, and 100-110 parts by weight of ultrapure water, put them into a stirring pot with a power of 1.8-2.2 kW, stir at a temperature of 20-25° C., stir for 8-12 minutes, and stir at a speed of 100-120 r / min to mix to obtain a pharmaceutically active solution;

[0018] L2, weighing 10-15 parts by weight of diallyl trisulfide, 18-26 parts of allicin, 10-20 parts of nasunin, and 25-35 parts of colloidal pectin bismuth, putting them into a stirring pot with a power of 1.8-2.2 kW, stirring at a temperature of 23-26° C., stirring for 7-10 min, and stirring at a speed of 100-110 r / min to obtain an active insect repellent;

[0019] L3. Add the active anthelmintic prepared in L2 into the active drug solution prepared in L1, stir at a temperature of 23-26°C, for 15-18 min, at a speed of 120-135 r / min, and stir to mix to obtain an anti-disease complex.

[0020] The present invention also proposes a method for preparing a fermented agarwood preparation for broiler chickens, which specifically comprises the following steps:

[0021] Step 1, putting the agarwood leaves into a dryer with a power of 1.3-1.8 kW, the drying temperature is 38-45°C, the drying time is 1.5-1.8 h, and after drying, using a powdering machine with a power of 1.6-2.2 kW, the powdering temperature is 25-28°C, the powdering time is 15-22 min, and after powdering, it is put into a fermentation tank with a power of 4.5-6.5 kW with a flavoring fermentation agent and a hygroscopic basic nutrient powder, and the stirring fermentation temperature is 37-42°C, the stirring fermentation time is 24-30 h, and the stirring fermentation speed is 120-140 r / min, and stirring and fermenting is performed to obtain a flavoring fermentation product;

[0022] Step 2: Put the flavor-corrected fermentation product prepared in step 1 into a puffing machine with a power of 6.2-7.5 kW, the puffing temperature is 150-170° C., and the puffing time is 30-40 min to obtain a modified nutritional puffed powder;

[0023] Step 3: Put the modified nutritional puffed powder prepared in step 2, the anti-disease complex, creatine water, sodium dehydroacetate, fenugreek saponin, L-alanyl-L-glutamine, and schisandra chinensis B into a stirring tank with a power of 1.2-1.5 kW, the stirring temperature is 20-25°C, the stirring time is 25-30 min, the stirring speed is 120-130 r / min, and after stirring and mixing, put it into a granulator with a power of 10-12 kW, the granulation temperature is 60-65°C, the granulation time is 40-55 min, the granulation size is 1.5-3.5 mm, and after granulation, air-dry at a temperature of 25-28°C for 2-3 h to obtain a fermented feeding agarwood preparation for broilers.

[0024] The beneficial effects achieved by the present invention are as follows:

[0025] The invention adopts a nutritional supplement mixed powder containing Yunnan sowberry, Artemisia selengensis and Brasenia schrenkiana to obtain an extract solution through ultrasonic extraction, adds cecropin and octaargine, obtains a modified extract solution under acidic conditions, and stirs and mixes it with a starch mixture obtained by drying and beating Polygonum aviculare, Agar-agar and brown rice. The prepared hygroscopic basic nutritional powder contains rich carbohydrates, proteins, minerals and trace elements required for the growth stage of broilers, is beneficial to the replication, activation and regeneration of various active enzymes in broilers, cooperates with creatine hydrate, fenugreek saponin and L-alanyl-L-glutamine, increases the water content in broiler muscles, reduces the decomposition of proteins in muscles, and improves the tenderness of broiler muscle fibers during the market period; sulforaphane, cabbage sulfonate and citric acid are mixed with an active anthelmintic containing diallyl trisulfide, allicin, nasunin and colloidal pectin bismuth, and the obtained anti-disease complex protects the gastrointestinal mucosa of broilers while , increase the anti-inflammatory and antiviral capabilities of pulmonary vascular endothelial cells in broiler chickens, reduce the damage of respiratory viral infections to the lungs, and can serve as an antagonist of parasite calmodulin, antagonizing the sites where calmodulin is activated by calcium ions to affect its binding with target enzymes, thereby killing parasites; agarwood leaves are mixed and fermented with flavor-correcting fermentation bacteria and hygroscopic basic nutrient powder to produce volatile flavor substances such as phenylethanol, ethyl caprylate, and ethyl 2-methylbutyrate, which increase the feed intake of broilers. After being puffed and modified by an extruder, the high temperature of the extruder can kill the bacteria in the fermentation process and mature the traditional Chinese medicine components therein. The increase in surface area after puffing further improves the moisture absorption capacity of the modified nutrient puffed powder, increases the contact area between the components, and makes the distribution more uniform. It cooperates with sodium dehydroacetate and schisandra chinensis B to keep the fermented agarwood preparation for broilers dry, free of mold, and with stable components. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the present invention or the technical solutions in the prior art, the following will be described in a clear and understandable manner in conjunction with the accompanying drawings. Obviously, the drawings described below are only for the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0027] Figure 1 This is the result diagram of mouse cytokine content described in Experimental Example 2 of the present invention;

[0028] Figure 2 This is a graph showing the cure rate of Ascaris lumbricoides in Experimental Example 2 of the present invention;

[0029] Figure 3 This is a graph showing the fungal colony count results described in Experimental Example 3 of the present invention. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0031] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0032] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials and test strains used in the following examples are purchased from commercial channels unless otherwise specified.

[0033] Example 1: This example provides a fermented agarwood preparation for broilers and a preparation method thereof. The fermented agarwood preparation for broilers comprises the following components in parts by weight:

[0034] 100 parts of agarwood leaves, 200 parts of hygroscopic basic nutrient powder, 70 parts of disease-resistant compound, 60 parts of flavor-correcting fermentation bacteria, 35 parts of creatine hydrate, 15 parts of sodium dehydroacetate, 12 parts of fenugreek saponin, 15 parts of L-alanyl-L-glutamine and 15 parts of schisandra chinensis B.

[0035] The flavor-correcting fermentation bacterial agent specifically includes: Corynebacterium glutamicum, Acinetobacter calcoaceticus, Enterobacter mulberry, and Tetradecanobacterium dodecanedigensis, with a weight ratio of 1:1:2:1.

[0036] The Corynebacterium glutamicum was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.15647; the Acinetobacter calcoaceticus was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.6186; the Enterobacter mulberryi was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.10322; and the Tetradecapsula dodecagenes was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.10746.

[0037] The preparation method of the hygroscopic basic nutritional powder specifically comprises the following steps:

[0038] S1, weighing 200 parts of Polygonum sibiricum, 120 parts of Agar-agar grass, and 150 parts of brown rice, putting them into a dryer with a power of 1.3 kW, a drying temperature of 65 ° C, and a drying time of 1 h, and after drying, using a powdering machine with a power of 1.6 kW, a powdering temperature of 25 ° C, and a powdering time of 20 min, to powder, and obtain a starch mixture;

[0039] S2, weighing 60 parts of Yunnan sorrel, 50 parts of Artemisia selengensis, and 40 parts of Brasenia schrenkiana by weight, and putting them into a vacuum freeze dryer with a power of 5.5 kW, the vacuum degree is 60 Pa, the freezing temperature is -20°C, the freezing time is 2 h, and then the temperature is raised to 2°C. After drying for 1.2 h, a powdering machine with a power of 1.6 kW is used, the powdering temperature is 25°C, and the powdering time is 20 min to obtain a nutritional supplement mixed powder;

[0040] S3, weighing 220 parts by weight of ultrapure water and the mixed powder of the nutritional supplement prepared in S2, putting them into an ultrasonic extractor with a power of 1.2 kW, an ultrasonic frequency of 20 MHz, an ultrasonic extraction temperature of 40° C., and an ultrasonic extraction time of 8 min, and performing ultrasonic extraction to obtain an extract solution;

[0041] S4, weighing 60 parts of cecropin, 30 parts of octaarginine, and 120 parts of ultrapure water, putting them into a stirring tank with a power of 1.2 kW, stirring at a temperature of 23°C, stirring for 10 min, stirring at a speed of 110 r / min, stirring and mixing, adjusting the pH to 6.2 with a 2% hydrochloric acid solution, and mixing with the extract solution prepared in S3 to obtain a modified extract solution;

[0042] S5. Put the starch mixture prepared by S1 into the modified extract solution prepared by S4, stir at a temperature of 25°C, stir for 12 min, and stir at a speed of 120 r / min. Stir evenly, put into a dryer with a power of 1.3 kW, and dry at a temperature of 35°C for 1.2 h. After drying, obtain the hygroscopic basic nutrient powder.

[0043] The method for preparing the anti-disease compound specifically comprises the following steps:

[0044] L1. Weigh 35 parts by weight of sulforaphane, 30 parts by weight of cabbage sulforaphane, 30 parts by weight of citric acid, and 100 parts by weight of ultrapure water, put them into a stirring pot with a power of 1.8 kW, stir at a temperature of 20° C., stir for 8 min, and stir at a speed of 100 r / min to mix to obtain a pharmaceutically active solution;

[0045] L2, weighing 10 parts of diallyl trisulfide, 18 parts of allicin, 10 parts of nasunin, and 25 parts of colloidal pectin bismuth, putting them into a stirring pot with a power of 1.8 kW, stirring at a temperature of 23°C, stirring for 7 min, and stirring at a speed of 100 r / min to obtain an active insect repellent;

[0046] L3. Put the active anthelmintic prepared in L2 into the active drug solution prepared in L1, stir at 23°C for 15 min and at 120 r / min, and mix by stirring to obtain an anti-disease complex.

[0047] This embodiment also provides a method for preparing a fermented agarwood preparation for broiler chickens, which specifically comprises the following steps:

[0048] Step 1, put the agarwood leaves into a dryer with a power of 1.3 kW, the drying temperature is 38°C, the drying time is 1.5 h, and after drying, use a powdering machine with a power of 1.6 kW, the powdering temperature is 25°C, the powdering time is 15 min, and after powdering, put it into a fermentation tank with a power of 4.5 kW, the stirring fermentation temperature is 37°C, the stirring fermentation time is 24 h, the stirring fermentation speed is 120 r / min, and stirring fermentation is performed to obtain a flavor-correcting fermentation product;

[0049] Step 2: Put the flavor-corrected fermentation product prepared in step 1 into a puffing machine with a power of 6.2 kW, the puffing temperature is 150° C., and the puffing time is 30 min to obtain a modified nutritional puffed powder;

[0050] Step 3: Put the modified nutritional puffed powder prepared in step 2 and the anti-disease complex, creatine water, sodium dehydroacetate, fenugreek saponin, L-alanyl-L-glutamine, and schisandra chinensis B into a stirring tank with a power of 1.2 kW, the stirring temperature is 20°C, the stirring time is 25 min, the stirring speed is 120 r / min, and after stirring and mixing, put it into a granulator with a power of 10 kW, the granulation temperature is 60°C, the granulation time is 40 min, the granulation size is 1.5 mm, and after granulation, air-dry at 25°C for 2 hours to obtain a fermented feeding agarwood preparation for broilers.

[0051] Example 2: This example provides a fermented agarwood preparation for broilers and a preparation method thereof. The fermented agarwood preparation for broilers comprises the following components in parts by weight:

[0052] 110 parts of agarwood leaves, 220 parts of hygroscopic basic nutrient powder, 75 parts of disease-resistant compound, 70 parts of flavor-correcting fermentation bacteria, 45 parts of creatine hydrate, 18 parts of sodium dehydroacetate, 16 parts of fenugreek saponin, 18 parts of L-alanyl-L-glutamine and 18 parts of schisandra chinensis B.

[0053] The flavor-correcting fermentation bacterial agent specifically includes: Corynebacterium glutamicum, Acinetobacter calcoaceticus, Enterobacter mulberry, and Tetradecanobacterium dodecanedigensis, and the mixing ratio by weight is 1.3:1.5:2.5:1.

[0054] The Corynebacterium glutamicum was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.15647; the Acinetobacter calcoaceticus was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.6186; the Enterobacter mulberryi was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.10322; and the Tetradecapsula dodecagenes was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.10746.

[0055] The preparation method of the hygroscopic basic nutritional powder specifically comprises the following steps:

[0056] S1, weighing 220 parts of Polygonum sibiricum, 135 parts of Agar grass, and 170 parts of brown rice in parts by weight, putting into a dryer with a power of 1.5 kW, the drying temperature is 68 ° C, and the drying time is 1.3 h. After drying, a powdering machine with a power of 1.9 kW is used, and the powdering temperature is 27 ° C, and the powdering time is 23 min. Powdering is performed to obtain a starch mixture;

[0057] S2, weighing 65 parts of Yunnan sorrel, 60 parts of Artemisia selengensis, and 45 parts of Brasenia schrenkiana by weight, and putting them into a vacuum freeze dryer with a power of 6.5 kW, the vacuum degree is 70 Pa, the freezing temperature is -20°C, the freezing time is 2.5 h, and then the temperature is raised to 3°C. After drying for 1.3 h, a powdering machine with a power of 1.9 kW is used, the powdering temperature is 26°C, and the powdering time is 23 min to obtain a nutritional supplement mixed powder;

[0058] S3, weighing 260 parts by weight of ultrapure water and the mixed powder of the nutritional supplement prepared in S2, putting them into an ultrasonic extractor with a power of 1.4 kW, an ultrasonic frequency of 25 MHz, an ultrasonic extraction temperature of 43° C., and an ultrasonic extraction time of 10 min, and performing ultrasonic extraction to obtain an extract solution;

[0059] S4, weighing 65 parts of cecropin, 40 parts of octaarginine, and 125 parts of ultrapure water, putting them into a stirring tank with a power of 1.3 kW, stirring at a temperature of 24°C, stirring for 13 min, stirring at a speed of 115 r / min, stirring and mixing, adjusting the pH to 6.2 with a 2% hydrochloric acid solution, and mixing with the extract solution prepared in S3 to obtain a modified extract solution;

[0060] S5. Put the starch mixture prepared by S1 into the modified extract solution prepared by S4, stir at 27°C, stir for 14 min, stir at 120 r / min, stir evenly, put into a dryer with a power of 1.6 kW, dry at 38°C, dry for 1.4 h, and obtain hygroscopic basic nutrient powder after drying.

[0061] The method for preparing the anti-disease compound specifically comprises the following steps:

[0062] L1. Weigh 38 parts by weight of sulforaphane, 35 parts by weight of cabbage sulforaphane, 35 parts by weight of citric acid, and 105 parts by weight of ultrapure water, put them into a stirring pot with a power of 2.1 kW, stir at a temperature of 23° C., stir for 10 min, and stir at a speed of 110 r / min to mix to obtain a pharmaceutically active solution;

[0063] L2, weighing 13 parts by weight of diallyl trisulfide, 22 parts of allicin, 15 parts of nasunin, and 30 parts of colloidal pectin bismuth, putting them into a stirring pot with a power of 2.1 kW, stirring at a temperature of 25°C, stirring for 9 min, and stirring at a speed of 100 r / min to obtain an active insect repellent;

[0064] L3. Put the active anthelmintic prepared in L2 into the active drug solution prepared in L1, stir at 25°C for 17 min and at 130 r / min, and mix by stirring to obtain an anti-disease complex.

[0065] This embodiment also provides a method for preparing a fermented agarwood preparation for broiler chickens, which specifically comprises the following steps:

[0066] Step 1, put the agarwood leaves into a dryer with a power of 1.6 kW, the drying temperature is 42°C, the drying time is 1.7 h, and after drying, use a powdering machine with a power of 1.8 kW, the powdering temperature is 27°C, the powdering time is 20 min, and after powdering, put it into a fermentation tank with a power of 5.5 kW, the stirring fermentation temperature is 40°C, the stirring fermentation time is 28 h, the stirring fermentation speed is 130 r / min, and stirring fermentation is performed to obtain a flavor-correcting fermentation product;

[0067] Step 2: Put the flavor-corrected fermentation product prepared in step 1 into a puffing machine with a power of 6.8 kW, the puffing temperature is 160° C., and the puffing time is 35 min to obtain a modified nutritional puffed powder;

[0068] Step 3: Put the modified nutritional puffed powder prepared in step 2 and the anti-disease complex, creatine water, sodium dehydroacetate, fenugreek saponin, L-alanyl-L-glutamine, and schisandra chinensis B into a stirring tank with a power of 1.4 kW, the stirring temperature is 23°C, the stirring time is 28 min, the stirring speed is 125 r / min, and after stirring and mixing, put it into a granulator with a power of 11 kW, the granulation temperature is 63°C, the granulation time is 50 min, the granulation size is 2.5 mm, and after granulation, air-dry at 27°C for 2.5 h to obtain a fermented feeding agarwood preparation for broilers.

[0069] Example 3: This example provides a fermented agarwood preparation for broilers and a preparation method thereof. The fermented agarwood preparation for broilers comprises the following components in parts by weight:

[0070] 120 parts of agarwood leaves, 240 parts of hygroscopic basic nutrient powder, 80 parts of disease-resistant compound, 80 parts of flavor-correcting fermentation bacteria, 50 parts of creatine hydrate, 20 parts of sodium dehydroacetate, 18 parts of fenugreek saponin, 20 parts of L-alanyl-L-glutamine and 20 parts of schisandra chinensis B.

[0071] The flavor-correcting fermentation bacterial agent specifically includes: Corynebacterium glutamicum, Acinetobacter calcoaceticus, Enterobacter mulberry, and Tetradecanobacterium dodecanedigensis, with a weight ratio of 1.5:2:3:1.

[0072] The Corynebacterium glutamicum was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.15647; the Acinetobacter calcoaceticus was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.6186; the Enterobacter mulberryi was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.10322; and the Tetradecapsula dodecagenes was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.10746.

[0073] The preparation method of the hygroscopic basic nutritional powder specifically comprises the following steps:

[0074] S1, weighing 230 parts of Polygonum sibiricum, 150 parts of Agar-agar grass, and 180 parts of brown rice in parts by weight, putting into a dryer with a power of 1.8 kW, a drying temperature of 70 ° C, and a drying time of 1.5 h, and after drying, using a powdering machine with a power of 2.2 kW, a powdering temperature of 28 ° C, and a powdering time of 25 min, and powdering to obtain a starch mixture;

[0075] S2, weighing 80 parts of Yunnan sorrel, 70 parts of Artemisia selengensis, and 50 parts of Brasenia schrenkiana by weight, and putting them into a vacuum freeze dryer with a power of 7.5 kW, the vacuum degree is 80 Pa, the freezing temperature is -20°C, the freezing time is 3 h, and then the temperature is raised to 4°C, and after drying for 1.5 h, a powdering machine with a power of 2.2 kW is used, the powdering temperature is 28°C, and the powdering time is 25 min, to obtain a nutritional supplement mixed powder;

[0076] S3, weighing 280 parts by weight of ultrapure water and the mixed powder of the nutritional supplement prepared in S2, putting them into an ultrasonic extractor with a power of 1.5 kW, an ultrasonic frequency of 30 MHz, an ultrasonic extraction temperature of 45° C., and an ultrasonic extraction time of 12 min, and performing ultrasonic extraction to obtain an extract solution;

[0077] S4, weighing 70 parts of cecropin, 50 parts of octaarginine, and 130 parts of ultrapure water, putting them into a stirring tank with a power of 1.5 kW, stirring at a temperature of 25°C, stirring for 15 min, stirring at a speed of 120 r / min, stirring and mixing, adjusting the pH to 6.2 with a 2% hydrochloric acid solution, and mixing with the extract solution prepared in S3 to obtain a modified extract solution;

[0078] S5. Put the starch mixture prepared by S1 into the modified extract solution prepared by S4, stir at a temperature of 28°C, stir for 16 min, and stir at a speed of 130 r / min. Stir evenly, put into a dryer with a power of 1.8 kW, and dry at a temperature of 42°C for 1.6 h. After drying, a hygroscopic basic nutrient powder is obtained.

[0079] The method for preparing the anti-disease compound specifically comprises the following steps:

[0080] L1. Weigh 40 parts by weight of sulforaphane, 40 parts by weight of cabbage sulforaphane, 40 parts by weight of citric acid, and 110 parts by weight of ultrapure water, put them into a stirring pot with a power of 2.2 kW, stir at a temperature of 25° C., stir for 12 min, and stir at a speed of 120 r / min to mix to obtain a pharmaceutically active solution;

[0081] L2, weighing 15 parts of diallyl trisulfide, 26 parts of allicin, 20 parts of nasunin, and 35 parts of colloidal pectin bismuth, putting them into a stirring pot with a power of 2.2 kW, stirring at a temperature of 26°C, stirring for 10 min, and stirring at a speed of 110 r / min to obtain an active insect repellent;

[0082] L3. Put the active anthelmintic prepared in L2 into the active drug solution prepared in L1, stir at 26°C for 18 min and at a speed of 135 r / min, and mix by stirring to obtain an anti-disease complex.

[0083] This embodiment also provides a method for preparing a fermented agarwood preparation for broiler chickens, which specifically comprises the following steps:

[0084] Step 1, put the agarwood leaves into a dryer with a power of 1.8 kW, the drying temperature is 45°C, the drying time is 1.8 h, and after drying, use a powdering machine with a power of 2.2 kW, the powdering temperature is 28°C, the powdering time is 22 min, and after powdering, put it into a fermentation tank with a power of 6.5 kW, the stirring fermentation temperature is 42°C, the stirring fermentation time is 30 h, the stirring fermentation speed is 140 r / min, and stirring fermentation is performed to obtain a flavor-correcting fermentation product;

[0085] Step 2: Put the flavor-corrected fermentation product prepared in step 1 into a puffing machine with a power of 7.5 kW, the puffing temperature is 170° C., and the puffing time is 40 min to obtain a modified nutritional puffed powder;

[0086] Step 3: Put the modified nutritional puffed powder prepared in step 2 and the anti-disease complex, creatine water, sodium dehydroacetate, fenugreek saponin, L-alanyl-L-glutamine, and schisandra chinensis B into a stirring tank with a power of 1.5 kW, the stirring temperature is 25°C, the stirring time is 30 min, the stirring speed is 130 r / min, and after stirring and mixing, put it into a granulator with a power of 12 kW, the granulation temperature is 65°C, the granulation time is 55 min, the granulation size is 3.5 mm, and after granulation, air-dry at 28°C for 3 hours to obtain a fermented feeding agarwood preparation for broilers.

[0087] Example 4: This example provides a fermented agarwood preparation for broilers and a preparation method thereof. The fermented agarwood preparation for broilers comprises the following components in parts by weight:

[0088] 106 parts of agarwood leaves, 210 parts of hygroscopic basic nutrient powder, 73 parts of disease-resistant compound, 65 parts of flavor-correcting fermentation bacteria, 38 parts of creatine hydrate, 16 parts of sodium dehydroacetate, 14 parts of fenugreek saponin, 17 parts of L-alanyl-L-glutamine and 16 parts of schisandra chinensis B.

[0089] The flavor-correcting fermentation bacterial agent specifically includes: Corynebacterium glutamicum, Acinetobacter calcoaceticus, Enterobacter mulberry, and Tetradecanobacterium dodecanedigensis, and the weight ratio of the mixture is 1.2:1.4:2.3:1.

[0090] The Corynebacterium glutamicum was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.15647; the Acinetobacter calcoaceticus was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.6186; the Enterobacter mulberryi was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.10322; and the Tetradecapsula dodecagenes was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.10746.

[0091] The preparation method of the hygroscopic basic nutritional powder specifically comprises the following steps:

[0092] S1, weighing 210 parts of Polygonum sibiricum, 125 parts of Agar grass, and 160 parts of brown rice in parts by weight, putting into a dryer with a power of 1.4 kW, the drying temperature is 67 ° C, the drying time is 1.2 h, and after drying, a powdering machine with a power of 1.7 kW is used, the powdering temperature is 26 ° C, and the powdering time is 22 min, and powdering is performed to obtain a starch mixture;

[0093] S2, weighing 63 parts of Yunnan sorrel, 55 parts of Artemisia selengensis, and 43 parts of Brasenia schrenkiana by weight, and putting them into a vacuum freeze dryer with a power of 6.2 kW, the vacuum degree is 60 Pa, the freezing temperature is -20°C, the freezing time is 2.2 h, and then the temperature is raised to 3°C. After drying for 1.2 h, a powdering machine with a power of 1.8 kW is used, the powdering temperature is 25°C, and the powdering time is 21 min to obtain a nutritional supplement mixed powder;

[0094] S3, weighing 230 parts by weight of ultrapure water and the mixed powder of the nutritional supplement prepared in S2, putting them into an ultrasonic extractor with a power of 1.3 kW, an ultrasonic frequency of 23 MHz, an ultrasonic extraction temperature of 42° C., and an ultrasonic extraction time of 9 min, and performing ultrasonic extraction to obtain an extract solution;

[0095] S4, weighing 63 parts of cecropin, 35 parts of octaarginine, and 123 parts of ultrapure water, putting them into a stirring tank with a power of 1.3 kW, stirring at a temperature of 24°C, stirring for 12 min, stirring at a speed of 110 r / min, stirring and mixing, adjusting the pH to 6.2 with a 2% hydrochloric acid solution, and mixing with the extract solution prepared in S3 to obtain a modified extract solution;

[0096] S5. Put the starch mixture prepared by S1 into the modified extract solution prepared by S4, stir at a temperature of 26°C, stir for 13 min, and stir at a speed of 120 r / min. Stir evenly, put into a dryer with a power of 1.5 kW, and dry at a temperature of 37°C for 1.3 h. After drying, obtain the hygroscopic basic nutrient powder.

[0097] The method for preparing the anti-disease compound specifically comprises the following steps:

[0098] L1. Weigh 37 parts by weight of sulforaphane, 33 parts by weight of cabbage sulforaphane, 34 parts by weight of citric acid, and 103 parts by weight of ultrapure water, put them into a stirring pot with a power of 1.9 kW, stir at a temperature of 22° C., stir for 9 min, and stir at a speed of 110 r / min to mix to obtain a pharmaceutically active solution;

[0099] L2, weighing 12 parts by weight of diallyl trisulfide, 20 parts of allicin, 13 parts of nasunin, and 28 parts of colloidal pectin bismuth, putting them into a stirring pot with a power of 1.9 kW, stirring at a temperature of 24°C, stirring for 8 min, and stirring at a speed of 100 r / min to obtain an active insect repellent;

[0100] L3. Put the active anthelmintic prepared in L2 into the active drug solution prepared in L1, stir at 24°C for 16 min and at a speed of 130 r / min, and stir to mix to obtain an anti-disease complex.

[0101] This embodiment also provides a method for preparing a fermented agarwood preparation for broiler chickens, which specifically comprises the following steps:

[0102] Step 1, put the agarwood leaves into a dryer with a power of 1.5 kW, the drying temperature is 40°C, the drying time is 1.6 h, and after drying, use a powdering machine with a power of 1.7 kW, the powdering temperature is 26°C, the powdering time is 18 min, and after powdering, put it into a fermentation tank with a power of 5.2 kW, the stirring fermentation temperature is 38°C, the stirring fermentation time is 26 h, the stirring fermentation speed is 130 r / min, and stirring fermentation is performed to obtain a flavor-correcting fermentation product;

[0103] Step 2: Put the flavor-corrected fermentation product prepared in step 1 into a puffing machine with a power of 6.3 kW, the puffing temperature is 155° C., and the puffing time is 32 min to obtain a modified nutritional puffed powder;

[0104] Step 3: Put the modified nutritional puffed powder prepared in step 2 and the anti-disease complex, creatine water, sodium dehydroacetate, fenugreek saponin, L-alanyl-L-glutamine, and schisandra chinensis B into a stirring tank with a power of 1.3 kW, the stirring temperature is 22°C, the stirring time is 27 min, the stirring speed is 120 r / min, and after stirring and mixing, put it into a granulator with a power of 11 kW, the granulation temperature is 62°C, the granulation time is 45 min, the granulation size is 2 mm, and after granulation, it is air-dried at 26°C for 2.3 hours to obtain a fermented feeding agarwood preparation for broilers.

[0105] Example 5: This example provides a fermented agarwood preparation for broilers and a preparation method thereof. The fermented agarwood preparation for broilers comprises the following components in parts by weight:

[0106] 115 parts of agarwood leaves, 230 parts of hygroscopic basic nutrient powder, 78 parts of disease-resistant compound, 75 parts of flavor-correcting fermentation bacteria, 48 parts of creatine hydrate, 19 parts of sodium dehydroacetate, 17 parts of fenugreek saponin, 19 parts of L-alanyl-L-glutamine and 19 parts of schisandra chinensis B.

[0107] The flavor correcting fermentation bacterial agent specifically includes: Corynebacterium glutamicum, Acinetobacter calcoaceticus, Enterobacter mulberry, and Tetradecanobacterium dodecanedigensis, and the mixing ratio by weight is 1.4:1.8:2.7:1.

[0108] The Corynebacterium glutamicum was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.15647; the Acinetobacter calcoaceticus was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.6186; the Enterobacter mulberryi was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.10322; and the Tetradecapsula dodecagenes was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.10746.

[0109] The preparation method of the hygroscopic basic nutritional powder specifically comprises the following steps:

[0110] S1, weigh 225 parts of Polygonum sibiricum, 145 parts of Agar grass, 175 parts of brown rice, put into a dryer with a power of 1.7 kW, the drying temperature is 69 ° C, the drying time is 1.4 h, and after drying, a powder beating machine with a power of 2.1 kW is used, the powder beating temperature is 27 ° C, and the powder beating time is 24 min, and powder is beaten to obtain a starch mixture;

[0111] S2, weighing 75 parts of Yunnan sorrel, 68 parts of Artemisia selengensis, and 49 parts of Brasenia schrenkiana by weight, and putting them into a vacuum freeze dryer with a power of 7.2 kW, the vacuum degree is 80 Pa, the freezing temperature is -20°C, the freezing time is 2.8 h, and then the temperature is raised to 3°C, and after drying for 1.4 h, a powdering machine with a power of 2.1 kW is used, the powdering temperature is 27°C, and the powdering time is 24 min, and powdering is performed to obtain a nutritional supplement mixed powder;

[0112] S3, weighing 270 parts by weight of ultrapure water and the mixed powder of the nutritional supplement prepared in S2, putting them into an ultrasonic extractor with a power of 1.4 kW, an ultrasonic frequency of 28 MHz, an ultrasonic extraction temperature of 44° C., and an ultrasonic extraction time of 11 min, and performing ultrasonic extraction to obtain an extract solution;

[0113] S4, weighing 68 parts of cecropin, 45 parts of octaarginine, and 128 parts of ultrapure water, putting them into a stirring tank with a power of 1.4 kW, stirring at a temperature of 24°C, stirring for 14 min, stirring at a speed of 120 r / min, stirring and mixing, adjusting the pH to 6.2 with a 2% hydrochloric acid solution, and mixing with the extract solution prepared in S3 to obtain a modified extract solution;

[0114] S5. Put the starch mixture prepared by S1 into the modified extract solution prepared by S4, stir at 27°C for 15 min, and stir at 130 r / min. Stir evenly, put into a dryer with a power of 1.7 kW, and dry at 40°C for 1.5 h. After drying, obtain the hygroscopic basic nutrient powder.

[0115] The method for preparing the anti-disease compound specifically comprises the following steps:

[0116] L1. Weigh 39 parts by weight of sulforaphane, 38 parts by weight of cabbage sulforaphane, 37 parts by weight of citric acid, and 108 parts by weight of ultrapure water, put them into a stirring pot with a power of 2.1 kW, stir at a temperature of 24° C., stir for 11 min, and stir at a speed of 110 r / min to mix to obtain a pharmaceutically active solution;

[0117] L2, weighing 14 parts of diallyl trisulfide, 25 parts of allicin, 18 parts of nasunin, and 33 parts of colloidal pectin bismuth, putting them into a stirring pot with a power of 2.1 kW, stirring at a temperature of 25°C, stirring for 9 min, and stirring at a speed of 110 r / min to obtain an active insect repellent;

[0118] L3. Put the active anthelmintic prepared in L2 into the active drug solution prepared in L1, stir at 25°C for 18 min and at 130 r / min, and mix by stirring to obtain an anti-disease complex.

[0119] This embodiment also provides a method for preparing a fermented agarwood preparation for broiler chickens, which specifically comprises the following steps:

[0120] Step 1, put the agarwood leaves into a dryer with a power of 1.7 kW, the drying temperature is 43°C, the drying time is 1.7 h, and after drying, use a powdering machine with a power of 2.1 kW, the powdering temperature is 27°C, the powdering time is 21 min, and after powdering, put it into a fermentation tank with a power of 6.2 kW, the stirring fermentation temperature is 41°C, the stirring fermentation time is 29 h, the stirring fermentation speed is 140 r / min, and stirring fermentation is performed to obtain a flavor-correcting fermentation product;

[0121] Step 2: Put the flavor-corrected fermentation product prepared in step 1 into a puffing machine with a power of 7.2 kW, the puffing temperature is 160° C., and the puffing time is 38 min to obtain a modified nutritional puffed powder;

[0122] Step 3: Put the modified nutritional puffed powder prepared in step 2 and the anti-disease complex, creatine water, sodium dehydroacetate, fenugreek saponin, L-alanyl-L-glutamine, and schisandra chinensis B into a stirring tank with a power of 1.5 kW, the stirring temperature is 24°C, the stirring time is 29 min, the stirring speed is 130 r / min, and after stirring and mixing, put it into a granulator with a power of 11 kW, the granulation temperature is 64°C, the granulation time is 52 min, the granulation size is 3 mm, and after granulation, it is air-dried at 27°C for 2.8 hours to obtain a fermented feeding agarwood preparation for broilers.

[0123] Experimental Example 1: Test for determining the quality of broiler meat.

[0124] The test steps for determining the effect of the fermented agarwood preparation for broiler chickens prepared in Examples 1-5 of the present invention on the quality of broiler chickens are as follows:

[0125] (1) 300 newly hatched yellow-feathered broilers were selected and divided into 6 groups, with 50 chickens in each group;

[0126] (2) The broiler groups were fed with the fermented agarwood preparations prepared in Examples 1-5 as the experimental group. After the broilers hatched, the fermented agarwood preparations were added at 10% of the broiler body weight for 5 times a day. After 28 days of feeding, the fermented agarwood preparations were added at 8% of the broiler body weight for 4 times a day. After 21 days of feeding, the fermented agarwood preparations were added at 4% of the broiler body weight for 3 times a day for 14 days. The broiler group fed with ordinary broiler feed according to the same feeding method and number of days was used as the CK control group.

[0127] (3) Yellow-feathered broilers can be marketed after being fed for 63 days. After being marketed, they are slaughtered. After the meat is marketed, the color (brightness, redness, and yellowness) of the left breast muscle of yellow-feathered broilers is measured using a meat color meter, and the pH value of the right breast muscle is measured using a pH value meter 45 minutes after slaughter. 45min The pH value was measured after taking part of the right pectoral muscle and placing it in a PE fresh-keeping plastic bag and sealing it at 4℃ for 24 h. 24h The values ​​were recorded in Table 1.

[0128] Table 1. Meat quality test table

[0129]

[0130] Result analysis: As shown in Table 1, the pH value of broilers fed with the fermented agarwood preparations prepared in Examples 1-5 was 24h and pH 45min The decrease value was lower than that of the control group, the drip loss rate of broiler muscle in Example 1-5 groups was lower than that in the control group, the muscle shear force was higher than that in the control group, and the brightness and redness of the muscle were significantly higher than those in the control group, indicating that the fermented feeding agarwood preparation for broilers described in the present invention can make the pH of the breast muscle of broilers more stable, the muscle color more vivid, the drip loss rate low, the shear force large, the breast muscle water-binding capacity strong, and the meat more tender and elastic.

[0131] Experimental Example 2: Disease resistance determination test.

[0132] The test steps for determining the effect of the fermented agarwood preparation for broiler feeding prepared in Examples 1-5 of the present invention on disease resistance are as follows:

[0133] (1) 60 C57BL / 6J mice weighing 25 g were selected and randomly divided into 6 groups of 10 mice each. After each mouse was anesthetized by intraperitoneal injection of 12.5 mg of tribromoethanol, 75 mg of lipopolysaccharide was administered intratracheally to the anesthetized mouse once a day. On the 7th day after the injection of lipopolysaccharide, the mice in experimental groups 1-5 were fed 10 g of the fermented agarwood preparation for broilers prepared in Example 1-5, once a day. The group fed with ordinary broiler feed was the CK control group;

[0134] (2) On the 21st day after LPS injection, the mice with lung injury model were killed by cervical dislocation, the chest cavity of the mice was opened, 100 mg of lung tissue was weighed, homogenized using a homogenizer, and centrifuged at 13,000 r / min for 10 min at 4°C, and the supernatant was collected;

[0135] (3) Use mouse interleukin 6 ELISA kit (ELISA, catalog number: ml098430), mouse interleukin 10 ELISA kit (ELISA, catalog number: ml037873), mouse interleukin 1β ELISA kit (ELISA, catalog number: ml059774), mouse transforming growth factor β1 ELISA kit (ELISA, catalog number: ml002115) to detect the levels of interleukin 6, interleukin 10, interleukin 1β, and transforming growth factor β1 in the supernatant of lung tissue;

[0136] (4) 120 30-day-old Hy-Line Brown chickens infected with Ascaris were selected and divided into 6 groups of 20 chickens each. The symptoms of Ascaris infection included depression, fluffy feathers, curled head, loss of appetite, fluid-filled glottis, pale comb and visible mucous membranes, emaciation, red carrot-like or brown feces, and bloody stools. The test showed that the chickens contained Ascaris or Ascaris eggs in their bodies. The average weight was 268 g per chicken.

[0137] (5) Each group of Hy-Line Brown chickens was raised separately to avoid inter-group infection. The fermented agarwood preparation prepared in Example 1-5 for broiler chickens was added at 8% of the body weight of the Hy-Line Brown chickens as the experimental group and fed 4 times a day. The Hy-Line Brown chicken group fed with ordinary broiler feed was used as the CK control group. After 7 days, the cure rate of ascariasis in Hy-Line Brown chickens was recorded.

[0138] Experimental Example 3: Anti-mildew ability determination test.

[0139] The test steps for determining the mildew resistance of the fermented agarwood preparation for broiler feeding prepared in Examples 1-5 of the present invention are as follows:

[0140] (1) Take 10 g of each of the fermented agarwood preparations for broiler chickens prepared in Examples 1-5 and the ordinary broiler feed for the control group, and take three replicates for each group;

[0141] (2) The fermented agarwood preparations for broiler feeding prepared in Examples 1-5 and the ordinary broiler feed of the control group were stored at a temperature of 40° C. and a humidity of 80%;

[0142] (3) Under the conditions of 40°C and 80% humidity, 1 g of the fermented agarwood preparation for broiler feeding prepared in Examples 1-5 and the ordinary broiler feed of the control group were taken respectively, and three parallel samples were taken from each group. The number of molds in the samples was measured every 5 days for 40 consecutive days;

[0143] (4) The fermented agarwood preparation for broiler feeding prepared in Examples 1-5 and the ordinary broiler feed of the control group were placed in a test tube containing 10 mL of sterile liquid beef extract peptone medium, and cultured in a shaker at 37°C and 120 r / min for 24 h to obtain a cultured bacterial liquid;

[0144] (5) Dilute the cultured bacterial solution 100 times and evenly spread it on a beef extract peptone solid culture medium plate in an ultra-clean workbench. Then invert and culture it in a 37°C constant temperature incubator for 24 h to obtain the number of mold colonies in each group. Calculate the number of mold colonies according to the formula: Total mold colony count (cfu / g) = number of colonies on each plate × dilution factor.

[0145] Result analysis:

[0146] Figure 1This is the result graph of mouse cytokine content described in Experimental Example 2 of the present invention. As shown in the figure, after the fermented agarwood preparation for broiler chickens described in Examples 1-5 was fed to lung injury model mice, the pro-inflammatory factor interleukin 6 content in the mouse lung tissue was 74.9 pg / mL, 73.6 pg / mL, 71.8 pg / mL, 72.5 pg / mL, and 74.4 pg / mL, the pro-inflammatory factor interleukin 1β content was 57.2 pg / mL, 55.4 pg / mL, 53.8 pg / mL, 54.9 pg / mL, and 55.1 pg / mL, the anti-inflammatory factor interleukin 10 content was 79.8 pg / mL, 81.4 pg / mL, 82.6 pg / mL, 81.9 pg / mL, and 82.3 pg / mL, and the anti-inflammatory factor transforming growth factor β1 content was 216.7 pg / mL, 217.6 pg / mL, 218.4 pg / mL, 217.9 pg / mL, and 218.2 pg / mL, while the content of pro-inflammatory factor interleukin-6 in the control group of mice was 126.8 pg / mL, the content of pro-inflammatory factor interleukin-1β was 127.9 pg / mL, the content of anti-inflammatory factor interleukin-10 was 48.6 pg / mL, and the content of anti-inflammatory factor transforming growth factor β1 was 94.7 pg / mL; Figure 2 This is a graph showing the cure rate of ascaris in Hailan Brown chickens as described in Experimental Example 2 of the present invention. As shown in the figure, the fermented agarwood preparation for broilers described in Examples 1-5 was fed to 30-day-old Hailan Brown chickens infected with ascaris. After 7 days, the cure rate of ascaris in Hailan Brown chickens was 100%, while the cure rate of ascaris in the control group was 6.7%. This shows that the fermented agarwood preparation for broilers prepared by the present invention can effectively repair lung damage and resist lung infection by inhibiting the production of pro-inflammatory factors and promoting the expression of anti-inflammatory factors, and can effectively fight ascaris in broilers.

[0147] Figure 3 This is a graph showing the fungal colony count results described in Experimental Example 3 of the present invention. As shown in the figure, after the fermented agarwood preparations for broiler chickens described in Examples 1-5 were stored at a temperature of 40°C and a humidity of 80% for 40 days, the total number of mold colonies produced was 7.41 cfu / g, 7.38 cfu / g, 7.37 cfu / g, 7.4 cfu / g, and 7.42 cfu / g, while the total number of mold colonies produced by the ordinary broiler feed in the control group was 115.3 cfu / g, indicating that the fermented agarwood preparations for broiler chickens prepared by the present invention can be stored in a high temperature and humid environment for a long time without becoming moldy and can exist stably.

[0148] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0149] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A fermented agarwood preparation for broiler chickens, characterized in that: The fermented agarwood preparation for broiler chickens specifically comprises the following raw materials in parts by weight: 100-120 parts of agarwood leaves, 200-240 parts of hygroscopic basic nutrient powder, 70-80 parts of disease-resistant compound, 60-80 parts of flavor-correcting fermentation bacteria, 35-50 parts of creatine hydrate, 15-20 parts of sodium dehydroacetate, 12-18 parts of fenugreek saponin, 15-20 parts of L-alanyl-L-glutamine, and 15-20 parts of schisandra chinensis B; The preparation method of the hygroscopic basic nutritional powder specifically comprises the following steps: S1, weighing 200-230 parts of Polygonum sibiricum, 120-150 parts of Agar-agar grass, and 150-180 parts of brown rice, drying and beating the mixture to obtain a starch mixture; S2, weighing 60-80 parts of Sophora flavescens, 50-70 parts of Artemisia selengensis, and 40-50 parts of Brasenia schrenkiana by weight, freeze-dried them in vacuum, ground them into powder, mixed them with 220-280 parts of ultrapure water, and subjected to ultrasonic extraction to obtain an extract solution; S3, weighing 60-70 parts of cecropin, 30-50 parts of octaarginine, and 120-130 parts of ultrapure water, stirring and mixing, adjusting the pH to 6.2 with a 2% hydrochloric acid solution, mixing with the extract solution prepared in S2, adding the starch mixture prepared in S1, stirring evenly, and drying to obtain a hygroscopic basic nutrient powder; The method for preparing the anti-disease compound specifically comprises the following steps: L1. Weigh 35-40 parts by weight of sulforaphane, 30-40 parts by weight of cabbage sulforaphane, 30-40 parts by weight of citric acid, and 100-110 parts by weight of ultrapure water, and stir and mix to obtain a pharmaceutically active solution; L2, weighing 10-15 parts by weight of diallyl trisulfide, 18-26 parts by weight of allicin, 10-20 parts by weight of nasunin, and 25-35 parts by weight of colloidal bismuth pectin, and stirring to obtain an active insect repellent; L3, dispersing the active insect repellent prepared in L2 in the active drug solution prepared in L1 to obtain an anti-disease complex.

2. The fermented agarwood preparation for broiler chickens according to claim 1, characterized in that: The flavor correcting fermentation bacterial agent specifically comprises: Corynebacterium glutamicum, Acinetobacter calcoaceticus, Enterobacter mulberry, and Tetradecanobacterium dodecanedigensis, and the mixing ratio by weight is 1-1.5:1-2:2-3:

1.

3. A method for preparing a fermented agarwood preparation for broiler chickens according to any one of claims 1-2, characterized in that: The specific steps include: The agarwood leaves are dried and powdered, mixed with a flavor-correcting fermentation bacteria agent and a hygroscopic basic nutrient powder, and stirred for fermentation. The stirred and fermented product is modified by a puffing machine, and then stirred and mixed with an anti-disease complex, creatine water, sodium dehydroacetate, fenugreek saponin, L-alanyl-L-glutamine, and schisandra chinensis B, granulated, and dried to obtain a fermented agarwood preparation for broiler feeding.

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