A skin whitening and repairing composition and its preparation method and application

Abstract

The present invention discloses a skin whitening and repairing composition, a preparation method and an application thereof, wherein the composition is composed of the following substances by mass: 2 to 5 parts of active extract component A, 1 to 3 parts of active extract component B, 10 to 48 parts of cyclodextrin, and 2 to 3 parts of hyaluronic acid. The active ingredients are included in cyclodextrin to improve the stability of the active ingredients and reduce the influence of environmental factors on the active ingredients. In addition, cyclodextrin can control the release rate of the active ingredients, so that the skin care products can achieve a longer-lasting effect.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a skin whitening and repairing composition, a preparation method and application thereof. Background Art

[0002] As consumers become more and more aware of the safety of skin care products, it has become a necessary behavior for many consumers to check the safety of ingredients and efficacy and texture before purchasing skin care products. Therefore, in order to meet the current consumer market demand, the use of safer natural ingredients to prepare skin care products has become one of the mainstream.

[0003] At present, natural active ingredients such as flavonoids are mainly extracted from traditional Chinese medicine, fruits or vegetables. However, these natural active ingredients are either volatile ingredients, unstable compounds, or ingredients that are easily affected by the environment, causing skin care products to evaporate, degrade or become inactivated when exposed to sunlight or high temperatures, thereby reducing the efficacy of the natural active ingredients on the skin. Summary of the invention

[0004] The present invention provides a skin whitening and repairing composition and a preparation method and application thereof, so as to solve the technical problem that the active ingredients in current skin care products are easily affected by the environment and become unstable.

[0005] In a first aspect, the present invention provides a skin whitening and repairing composition, which is composed of the following substances in parts by mass:

[0006] 2 to 5 parts of active extract component A, 1 to 3 parts of active extract component B, 10 to 48 parts of cyclodextrin, and 2 to 3 parts of hyaluronic acid;

[0007] The active extract component A is obtained by extracting the following raw materials in the following mass ratio: Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense and Forsythia suspensa = (4-7): (1-3): (2-5): (2-5): (2-5);

[0008] The active extract component B is extracted from the following raw materials in the mass ratio: purple cabbage, tomato, pomegranate, lemon and grapefruit = (2-5): (4-6): (2-5): (5-9): (2-5).

[0009] The Scleromitrion diffusa of the present invention diffusum (Willd.) RJ Wang) is a heat-clearing medicine. It is the whole herb of Hedyotis diffusa, a plant of the genus Hedyotis of the Rubiaceae family. It contains calendula glycoside, calendula acid, deacetic acid, calendula acid, scutellaria glycoside, scutellaria glycoside methyl ester, 6-O-p-hydroxycinnamoyl calendula glycoside methyl ester, 6-O-p-methoxycinnamoyl calendula glycoside methyl ester, 6-O-feruloyl calendula glycoside methyl ester, 2-methyl-3-hydroxyanthraquinone, 2-methyl-3-methoxyanthraquinone, 2-methyl-3-hydroxy-4-methoxyanthraquinone, ursolic acid, β-sitosterol, hexadecane, stigmasterol, oleanolic acid, β-sitosterol-β-glucoside and p-coumaric acid. The main effects are clearing away heat and detoxifying, relieving pain and dispersing stagnation, and promoting diuresis and removing dampness. Indications: lung heat, wheezing and coughing, sore throat, intestinal carbuncle, furuncle, ulcer, snake bite, hot and painful stranguria, edema, dysentery, enteritis, damp-heat jaundice, and good at treating various cancers.

[0010] The Sophora flavescens Aiton of the present invention is a heat-clearing medicine, which is the dried root of the leguminous plant Sophora flavescens, and mainly contains matrine, which has an inhibitory effect on various skin fungi and tuberculosis bacteria. Sophora flavescens has a bitter and cold taste, can treat abdominal qi stagnation, abdominal masses, accumulation, jaundice, urine with residual drip, water expelling, carbuncle, can nourish the middle, improve eyesight and stop tears.

[0011] The Ophiopogon japonicus (Lf) Ker Gawl. of the present invention is a yin nourishing medicine, which is a dried root tuber of the Liliaceae plant Ophiopogon japonicus, which contains scutellaria glycosides, steroidal saponins, alkaloids, sitosterol, glucose, amino acids, vitamins, etc., and has the effects of anti-fatigue, scavenging free radicals, improving cellular immune function and lowering blood sugar. In addition, Ophiopogon japonicus has the effects of sedation, hypnosis, anti-myocardial ischemia, anti-arrhythmia, anti-tumor, etc., and has multiple effects on improving the health of the elderly.

[0012] The bupleurum root described in the present invention is a medicine for dispelling exterior pathogens, which is the dried root of the umbelliferae plant bupleurum root or bupleurum angustifolia, and contains saponin components, volatile oil, polysaccharide, organic acid, phytosterol and flavonoid components, and has the effects of dispersing fever, relieving liver depression and lifting yang energy. It is mainly used to treat colds and fever, alternating cold and heat, chest and flank distension and pain, irregular menstruation, uterine prolapse and rectal prolapse.

[0013] The forsythia suspensa (Thunb.) Vahl of the present invention is a heat-clearing and detoxifying medicine, which is the dried fruit of the forsythia suspensa of the family Oleaceae, contains triterpenoid saponins, sterols, forsythia phenol, alkaloids, saponins, oleanolic acid, coumarins, rich vitamin P and a small amount of volatile oil, has the functions of clearing heat and detoxifying, relieving swelling and dispersing knots, and evacuating wind-heat. It is mainly used to treat carbuncle, scrofula, mastitis, erysipelas, wind-heat cold, early stage of febrile disease, warm and heat entering the camp, high fever and thirst, coma and spots, and hot stranguria and astringent pain.

[0014] The purple cabbage of the present invention is also called red cabbage, red cabbage, commonly known as purple cabbage, a variety of the Brassica cabbage species of the Cruciferae family, and a type of cabbage. The purple cabbage has purple-red leaves, wax powder on the leaf surface, and a nearly round head. The nutritional components of the purple cabbage include carbohydrates, proteins, vitamins such as folic acid, ascorbic acid, vitamin A, tocopherol, and minerals such as copper, iron, selenium, calcium, manganese, and zinc.

[0015] The tomato described in the present invention is an annual herbaceous plant of the genus Solanum of the Solanaceae family, is rich in carotene, vitamin C and B vitamins, has high nutritional value, can be used as both a vegetable and a fruit, and can be eaten raw or cooked; the tomato can be used to process and make tomato sauce, tomato juice and diced tomatoes, etc.; the tomato is rich in lycopene, has a strong antioxidant capacity, can lower blood pressure, and has the effects of clearing away heat and detoxifying, and can also be extracted as a health product.

[0016] The pomegranate of the present invention is a deciduous shrub or tree of the genus Punica of the Lythraceae family, which is sweet, sour, astringent and warm in nature and has the effects of insecticide, astringency, bowel astringency and dysentery relieving. The pomegranate fruit is rich in nutrition, and the vitamin C content is one to two times higher than that of apples and pears.

[0017] The lemon described in the present invention is a woody plant of the genus Citrus of the Rutaceae family, contains nicotinic acid and rich organic acids, has a very sour taste, and citric acid juice has a strong bactericidal effect. Fresh lemons are extremely rich in vitamins and are a natural beauty product that can prevent and eliminate skin pigmentation and has a whitening effect.

[0018] The grapefruit described in the present invention is a woody plant of the genus Citrus of the Rutaceae family, and contains cellulose, pectin, potassium, vitamin C, folic acid, inositol, bioflavonoids, limonene, etc., among which the vitamin C content is extremely rich, which can promote antibody production and enhance the detoxification function of the human body, and vitamin P can enhance skin elasticity and shrink pores.

[0019] The cyclodextrin (CD) described in the present invention is a general term for a series of cyclic oligosaccharides generated by amylose under the action of cyclodextrin glucosyltransferase produced by Bacillus, and usually contains 6 to 12 D-pyranose units. The cyclodextrin molecule has a slightly conical hollow cylindrical three-dimensional ring structure. In its hollow structure, the upper end (larger opening end) of the outer side is composed of secondary hydroxyl groups of C2 and C3, and the lower end (smaller opening end) is composed of primary hydroxyl groups of C6, which are hydrophilic, and a hydrophobic area is formed in the cavity due to the shielding effect of the CH bond. It has neither reducing ends nor non-reducing ends and is not reducing; it is very stable in alkaline media but can be cleaved by strong acids; it can only be hydrolyzed by α-amylase but not by β-amylase, and its tolerance to acid and general amylases is stronger than that of straight-chain starch; it can crystallize well in aqueous solution and alcohol-water solution; it has no fixed melting point and begins to decompose when heated to about 200°C, and has good thermal stability; it is not hygroscopic, but can easily form various stable hydrates; various organic compounds can be embedded in its hydrophobic cavities to form inclusion complexes and change the physical and chemical properties of the included complexes.

[0020] The hyaluronic acid described in the present invention is also called hyaluronic acid, and has a molecular formula of (C14H21NO11)n. It is a disaccharide unit glycosaminoglycan composed of D-glucuronic acid and N-acetylglucosamine.

[0021] Preferably, the mass ratio of the active extract component A, the active extract component B and cyclodextrin is 2:1:(4-6).

[0022] Preferably, the cyclodextrin is a mixture of α-cyclodextrin, β-cyclodextrin and γ-cyclodextrin in a mass ratio of 1:(5-7):(2-3).

[0023] Preferably, the mass ratio of Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense and Forsythia suspensa is 6.5:1.8:4.6:4.6:5.

[0024] Preferably, the mass ratio of the purple cabbage, tomato, pomegranate, lemon and grapefruit is 4.8:5.5:3.7:4.6:1.8.

[0025] In a second aspect, the present invention further provides a method for preparing the skin whitening and repairing composition as described in the first aspect, comprising:

[0026] Preparation of active extract component A: weigh Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense and Forsythia suspensa according to the mass ratio, wash, dry and crush, add ethanol solution, ultrasonically treat for 30min to 60min at a temperature of 20°C to 30°C and an ultrasonic frequency of 45kHz to 90kHz, take the supernatant and centrifuge, filter and reflux ethanol to obtain active extract component A;

[0027] Preparation of active extract component B: purple cabbage, tomato, pomegranate, lemon and grapefruit are weighed according to the mass ratio, washed, cut into pieces, squeezed and filtered to obtain filtrate a and filter residue; ethanol aqueous solution is added to the filter residue, ultrasonically treated at a temperature of 20°C to 30°C for 15min to 45min, the ultrasonic frequency is 30kHz to 60kHz, and filtrate b is obtained by filtering; filtrate a and filtrate b are mixed and centrifuged, and ethanol is refluxed to obtain active extract component B;

[0028] Preparation of a skin whitening and repairing composition: Weigh active extract component A, active extract component B, cyclodextrin and hyaluronic acid according to their mass fractions; at 40°C to 60°C, mix and stir the active extract component A, active extract component B and cyclodextrin at a speed of 1000rpm to 1200rpm for 20min to 40min, then add hyaluronic acid and continue stirring for 5min to 10min to obtain a skin whitening and repairing composition.

[0029] Preferably, in the preparation step of the active extract component A: the ultrasonic time is 45 min, and the ultrasonic frequency is 60 kHz;

[0030] In the preparation step of the active extract component B: the ultrasonic time is 25 min, and the ultrasonic frequency is 50 kHz;

[0031] In the preparation steps of the skin whitening and repairing composition: the stirring speed is 1000 rpm, the mixing and stirring time is 30 minutes, and the continuous stirring time is 8 minutes.

[0032] Preferably, the concentration of the ethanol solution in the step of preparing the active extract component A is 80%; the concentration of the ethanol aqueous solution in the step of preparing the active extract component B is 50%.

[0033] In a third aspect, the present invention further provides a use of the skin whitening and repairing composition as described in the first aspect in the preparation of cosmetics, wherein the cosmetics include facial masks, facial creams, lotions and essences.

[0034] Preferably, the skin whitening and repairing composition is used in an amount of 3% to 5% in the cosmetic.

[0035] Compared with the prior art, the present invention has the following beneficial effects:

[0036] 1. The active extract component A and the active extract component B are encapsulated by cyclodextrin to improve the stability of the active extract components and reduce the impact of environmental factors on the active ingredients. In addition, cyclodextrin can control the release rate of the active ingredients, so that the skin care products can achieve a longer-lasting effect.

[0037] 2. Extract active extract component A from Chinese medicine raw materials, extract active extract component B from vegetable and fruit raw materials, and then use active extract components A and B for compatibility, use Hedyotis diffusa as the main drug component to improve the skin's immunity such as antibacterial and anti-inflammatory; use Sophora flavescens and Ophiopogon japonicus as auxiliary drug components to further improve the skin's antibacterial and anti-inflammatory ability and make the skin have antioxidant and moisturizing effects; use Bupleurum and Forsythia suspensa as adjuvant components to further improve the skin's antibacterial and anti-inflammatory ability, reduce skin inflammation and infection, and regulate skin pigmentation and improve uneven skin color; use purple cabbage, tomato, pomegranate, lemon and grapefruit as adjuvant components to further improve the skin's ability to resist free radical damage and keep the skin healthy and young. The present invention adopts the Chinese medicine theory of monarch, minister, assistant and envoy (not limited to Chinese medicine components) for compatibility, improves the effect of each active ingredient, and has a significant synergistic effect in skin antibacterial and anti-inflammatory and whitening and repair.

[0038] 3. The preparation method of the present invention can effectively extract the active ingredients of each raw material, and make the active ingredients interact and cooperate with each other, and cyclodextrin can effectively include the active ingredients to improve the efficiency of the active ingredients. DETAILED DESCRIPTION

[0039] In order to make the above-mentioned purpose, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0040] As used herein, the term "prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0041] When amount, concentration or other value or parameter is expressed as range, preferred range or a series of upper preferred value and lower preferred value limit range, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value and any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when disclosing range "1 to 5", described range should be interpreted as including range "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5" etc. When numerical range is described in this article, unless otherwise stated, the range is intended to include its end value and all integers and fractions within the range.

[0042] In addition, the indefinite articles "a" and "an" before the elements or components of the present invention have no restrictions on the quantity requirements (i.e. the number of occurrences) of the elements or components. Therefore, "a" or "an" should be interpreted as including one or at least one, and the elements or components in the singular form also include the plural form, unless the number is obviously intended to be in the singular form.

[0043] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0044] Example 1

[0045] A skin whitening and repairing composition, comprising the following substances in parts by mass:

[0046] 2 parts of active extract component A, 1 part of active extract component B, 10 parts of cyclodextrin, and 2 parts of hyaluronic acid; wherein the cyclodextrin is a mixture of α-cyclodextrin, β-cyclodextrin and γ-cyclodextrin in a mass ratio of 1:5:2;

[0047] The active extract component A is obtained by extracting the following raw materials in a mass ratio: Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense and Forsythia suspensa = 4:1:2:2:5;

[0048] The active extract component B is extracted from the following raw materials in a mass ratio: purple cabbage, tomato, pomegranate, lemon and grapefruit = 2:4:2:5:2.

[0049] The preparation method comprises:

[0050] Preparation of active extract component A: weigh Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense and Forsythia suspensa according to the mass ratio, wash, dry and crush, add 80% ethanol solution, ultrasonically treat for 60 minutes at a temperature of 20°C and an ultrasonic frequency of 45kHz, take the supernatant and centrifuge, filter and reflux ethanol to obtain active extract component A;

[0051] Preparation of active extract component B: purple cabbage, tomato, pomegranate, lemon and grapefruit are weighed according to the mass ratio, washed, cut into pieces, squeezed and filtered to obtain filtrate a and filter residue; 50% ethanol aqueous solution is added to the filter residue, ultrasonically treated at a temperature of 20° C. for 15 minutes with an ultrasonic frequency of 60 kHz, and filtered to obtain filtrate b; filtrate a and filtrate b are mixed and centrifuged, and ethanol is refluxed to obtain active extract component B;

[0052] Preparation of a skin whitening and repairing composition: Weigh active extract component A, active extract component B, cyclodextrin and hyaluronic acid according to their mass fractions; at 40°C, mix and stir the active extract component A, active extract component B and cyclodextrin at a speed of 1000 rpm for 40 minutes, then add hyaluronic acid and continue stirring for 5 minutes to obtain a skin whitening and repairing composition.

[0053] Example 2

[0054] A skin whitening and repairing composition, comprising the following substances in parts by mass:

[0055] 5 parts of active extract component A, 3 parts of active extract component B, 36 parts of cyclodextrin, and 3 parts of hyaluronic acid; wherein the cyclodextrin is a mixture of α-cyclodextrin, β-cyclodextrin and γ-cyclodextrin in a mass ratio of 1:7:3;

[0056] The active extract component A is obtained by extracting the following raw materials in a mass ratio: Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense and Forsythia suspensa = 7:3:5:5:5;

[0057] The active extract component B is extracted from the following raw materials in a mass ratio: purple cabbage, tomato, pomegranate, lemon and grapefruit = 5:6:5:9:5.

[0058] The preparation method comprises:

[0059] Preparation of active extract component A: weigh Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense and Forsythia suspensa according to the mass ratio, wash, dry and crush, add 80% ethanol solution, ultrasonically treat for 30 minutes at a temperature of 30°C and an ultrasonic frequency of 90kHz, take the supernatant and centrifuge, filter and reflux ethanol to obtain active extract component A;

[0060] Preparation of active extract component B: purple cabbage, tomato, pomegranate, lemon and grapefruit are weighed according to the mass ratio, washed, cut into pieces, squeezed and filtered to obtain filtrate a and filter residue; 50% ethanol aqueous solution is added to the filter residue, ultrasonically treated at a temperature of 30° C. for 45 minutes with an ultrasonic frequency of 30 kHz, and filtered to obtain filtrate b; filtrate a and filtrate b are mixed and centrifuged, and ethanol is refluxed to obtain active extract component B;

[0061] Preparation of a skin whitening and repairing composition: Weigh active extract component A, active extract component B, cyclodextrin and hyaluronic acid according to their mass fractions; at 60°C, mix and stir the active extract component A, active extract component B and cyclodextrin at a speed of 1200 rpm for 20 minutes, then add hyaluronic acid and continue stirring for 10 minutes to obtain a skin whitening and repairing composition.

[0062] Example 3

[0063] A skin whitening and repairing composition, comprising the following substances in parts by mass:

[0064] 3.6 parts of active extract component A, 1.8 parts of active extract component B, 30 parts of cyclodextrin, and 2.5 parts of hyaluronic acid; wherein the cyclodextrin is a mixture of α-cyclodextrin, β-cyclodextrin and γ-cyclodextrin in a mass ratio of 1:6:2;

[0065] The active extract component A is obtained by extracting the following raw materials in a mass ratio: Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense and Forsythia suspensa = 6.5:1.8:4.6:4.6:5;

[0066] The active extract component B is extracted from the following raw materials in a mass ratio: purple cabbage, tomato, pomegranate, lemon and grapefruit = 4.8:5.5:3.7:4.6:1.8.

[0067] The preparation method comprises:

[0068] Preparation of active extract component A: weigh Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense and Forsythia suspensa according to the mass ratio, wash, dry and crush, add 80% ethanol solution, ultrasonically treat for 45 minutes at a temperature of 25°C and an ultrasonic frequency of 60kHz, take the supernatant and centrifuge, filter and reflux ethanol to obtain active extract component A;

[0069] Preparation of active extract component B: purple cabbage, tomato, pomegranate, lemon and grapefruit are weighed according to the mass ratio, washed, cut into pieces, squeezed and filtered to obtain filtrate a and filter residue; 50% ethanol aqueous solution is added to the filter residue, ultrasonically treated at a temperature of 25° C. for 25 minutes with an ultrasonic frequency of 50 kHz, and filtered to obtain filtrate b; filtrate a and filtrate b are mixed and centrifuged, and ethanol is refluxed to obtain active extract component B;

[0070] Preparation of skin whitening and repairing composition: Weigh active extract component A, active extract component B, cyclodextrin and hyaluronic acid according to their mass fractions; at 45°C, mix active extract component A, active extract component B and cyclodextrin at a speed of 1000pm for 30 minutes, then add hyaluronic acid and continue stirring for 8 minutes to obtain a skin whitening and repairing composition.

[0071] Comparative Example 1

[0072] Compared with Example 3, Comparative Example 1 does not contain cyclodextrin.

[0073] Comparative Example 2

[0074] Compared with Example 3, the cyclodextrin in Comparative Example 2 is β-cyclodextrin.

[0075] Comparative Example 3

[0076] Compared with Example 3, Comparative Example 3 does not contain Hedyotis diffusa.

[0077] Comparative Example 4

[0078] Compared with Example 3, Comparative Example 4 does not contain Sophora flavescens and Ophiopogon japonicus.

[0079] Comparative Example 5

[0080] Compared with Example 3, Comparative Example 5 does not contain Bupleurum and Forsythia.

[0081] Comparative Example 6

[0082] Compared with Example 3, Comparative Example 6 does not contain active extract component B.

[0083] (I) Test of scavenging ability of hydroxyl free radicals

[0084] Add 3 mL of 2 mmol / L FeSO4 and 3 mL of 1 mmol / L H2O2 to a 25 mL colorimetric tube, mix well, and then add 3 mL of 6 mmol / L salicylic acid. Heat in a 37°C water bath for 15 min, then take out and test the absorbance A0. Then add 100 mg·L -1 Take another colorimetric tube and repeat the above method, but without adding salicylic acid. After heating in a water bath for 15 minutes, measure the absorbance Ax0. Calculate the hydroxyl radical scavenging rate:

[0085] Hydroxyl radical scavenging rate (%) = A0-(Ax-Ax0) / A0×100%; wherein A0 is the absorbance value of the blank control, Ax is the absorbance value after adding the sample, and Ax0 is the absorbance value of the background of the H2O2 solution without adding the color developing agent. The test results are shown in Table 1.

[0086] (II) Test for inhibition of tyrosinase

[0087] 1 mL of phosphate buffer (PH=6.8), 1 mL of the composition sample, and 1 mL of L-tyrosine solution were added to the test tube of the sample group, and 2 mL of phosphate buffer (PH=6.8) and 1 mL of L-tyrosine solution were added to the test tube of the blank group and kept constant in a water bath at 37°C for 10 min. Then, 0.5 mL of tyrosinase was added to the test tube and kept constant for 15 min. Then, the absorbance value was measured at 475 nm, and the tyrosinase activity inhibition rate was calculated:

[0088] Tyrosinase activity inhibition rate (%) = (blank group absorbance - sample group absorbance) / blank group absorbance. The test results are shown in Table 1.

[0089] (III) Anti-inflammatory test

[0090] Take the Raw264.7 cells in the best growth state and dilute them to 7×10 4 ~9×10 4 100 μL of bacterial suspension per well was inoculated into a 96-well plate, cultured in a 37°C, 5% CO2 incubator, and treated with a composition at a mass concentration of 0.25% wt for 24 hours as a sample group, and a blank group without the composition was used. The supernatant was taken and the inhibitory effect on interleukin was determined using an ELISA kit, and the absorbance was measured at 450 nm using an enzyme reader to calculate the anti-inflammatory rate:

[0091] Anti-inflammatory rate (%) = (blank group absorbance - sample group absorbance) / blank group absorbance. The test results are shown in Table 1.

[0092] Table 1

[0093]

[0094]

[0095] As shown in Table 1, Examples 1 to 3 use Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense, Forsythia suspensa, purple cabbage, tomato, pomegranate, lemon and grapefruit to extract active ingredients, which can play a synergistic role in skin antibacterial and anti-inflammatory, whitening and repair. The effect of whether or not cyclodextrin is added and the type of cyclodextrin added in the non-skin test without contact with the skin is not significantly different.

[0096] (IV) Volunteer Testing

[0097] Test samples: facial creams prepared in Examples 1 to 3 and Comparative Examples 1 to 6, the facial creams are shown in Table 2 in terms of mass percentage.

[0098] Table 2

[0099]

[0100]

[0101] Test subjects: female volunteers aged 18 to 30 years old, with a small amount of acne on the face (the total area of ​​acne is less than 5 cm2), 5 people were assigned to each embodiment or comparative example, a total of 45 people.

[0102] Test steps:

[0103] 1. The subjects cleaned their faces with commercially available amino acid cleansing products, dried their skin with a face towel, and sat quietly in a waiting room with a temperature of 25±1℃ and a humidity of 50±10%RH for more than 30 minutes; the subjects' facial images were taken, and the basic gloss value of the subjects' faces was measured using a gloss probe, and the basic chromaticity value of the subjects' faces was measured using a skin colorimeter.

[0104] 2. The subjects used the cream once in the morning and once in the evening every day in the same way. The subjects took a facial image every morning and measured the facial gloss and facial chroma. According to the facial image, the number of days required for acne reduction (acne color lightening rate greater than 80%) was counted; according to the facial gloss, the number of days required for brightening the skin tone (skin brightness improvement rate greater than 40%) was counted; according to the facial chroma, the number of days required for whitening (skin whiteness improvement rate greater than 40%) was counted. The test results are shown in Table 3.

[0105] Table 3

[0106]

[0107]

[0108] As shown in Table 3, Examples 1 to 3 use Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense, Forsythia suspensa, purple cabbage, tomato, pomegranate, lemon and grapefruit to extract active ingredients, which can play a synergistic role in skin antibacterial and anti-inflammatory, whitening and color enhancement. Cyclodextrin inclusion of active ingredients can improve the effect of active ingredients, and using α-cyclodextrin, β-cyclodextrin and γ-cyclodextrin as inclusion matrix can improve the inclusion effect.

[0109] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0110] The specific embodiments described above further illustrate the purpose, technical solutions and beneficial effects of the present invention. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the scope of protection of the present invention. It is particularly pointed out that for those skilled in the art, any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the scope of protection of the present invention.

Claims

1. A skin whitening and repairing composition, characterized in that: It is composed of the following substances in parts by mass: 2 to 5 parts of active extract component A, 1 to 3 parts of active extract component B, 10 to 48 parts of cyclodextrin, and 2 to 3 parts of hyaluronic acid; The active extract component A is obtained by extracting the following raw materials in the following mass ratio: Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense and Forsythia suspensa = (4-7): (1-3): (2-5): (2-5): (2-5); The active extract component B is extracted from the following raw materials in the mass ratio: purple cabbage, tomato, pomegranate, lemon and grapefruit = (2-5): (4-6): (2-5): (5-9): (2-5); A method for preparing a skin whitening and repairing composition, comprising: Preparation of active extract component A: weigh Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense and Forsythia suspensa according to the mass ratio, wash, dry and crush, add ethanol solution, ultrasonically treat for 30min to 60min at a temperature of 20°C to 30°C and an ultrasonic frequency of 45kHz to 90kHz, take the supernatant and centrifuge, filter and reflux ethanol to obtain active extract component A; Preparation of active extract component B: purple cabbage, tomato, pomegranate, lemon and grapefruit are weighed according to the mass ratio, washed, cut into pieces, squeezed and filtered to obtain filtrate a and filter residue; ethanol aqueous solution is added to the filter residue, ultrasonically treated at a temperature of 20°C to 30°C for 15min to 45min, the ultrasonic frequency is 30kHz to 60kHz, and filtrate b is obtained by filtering; filtrate a and filtrate b are mixed and centrifuged, and ethanol is refluxed to obtain active extract component B; Preparation of a skin whitening and repairing composition: Weigh active extract component A, active extract component B, cyclodextrin and hyaluronic acid according to their mass fractions; at 40°C to 60°C, mix and stir the active extract component A, active extract component B and cyclodextrin at a speed of 1000rpm to 1200rpm for 20min to 40min, then add hyaluronic acid and continue stirring for 5min to 10min to obtain a skin whitening and repairing composition.

2. The skin whitening and repairing composition according to claim 1, characterized in that: The mass ratio of the active extract component A, the active extract component B and cyclodextrin is 2:1:(4-6).

3. The skin whitening and repairing composition according to claim 1, characterized in that: The cyclodextrin is a mixture of alpha cyclodextrin, beta cyclodextrin and gamma cyclodextrin in a mass ratio of 1:(5-7):(2-3).

4. The skin whitening and repairing composition according to claim 1, characterized in that: The mass ratio of Hedyotis diffusa, Sophora flavescens, Ophiopogon japonicus, Bupleurum chinense and Forsythia suspensa is 6.5:1.8:4.6:4.6:

5.

5. The skin whitening and repairing composition according to claim 1, characterized in that: The mass ratio of the purple cabbage, tomato, pomegranate, lemon and grapefruit is 5:6:5:9:

5.

6. The skin whitening and repairing composition according to claim 1, characterized in that: In the preparation steps of the active extract component A: the ultrasonic time is 45 min, and the ultrasonic frequency is 60 kHz; In the preparation step of the active extract component B: the ultrasonic time is 25 min, and the ultrasonic frequency is 50 kHz; In the preparation steps of the skin whitening and repairing composition: the stirring speed is 1000 rpm, the mixing and stirring time is 30 minutes, and the continuous stirring time is 8 minutes.

7. The skin whitening and repairing composition according to claim 1, characterized in that: The concentration of the ethanol solution in the step of preparing the active extract component A is 80%; the concentration of the ethanol aqueous solution in the step of preparing the active extract component B is 50%.

8. Use of the skin whitening and repairing composition according to any one of claims 1 to 7 in the preparation of cosmetics, characterized in that: The cosmetics include facial masks, facial creams, lotions and essences.

9. Use of the skin whitening and repairing composition according to claim 8 in preparing cosmetics, characterized in that: The percentage usage of the skin whitening and repairing composition in the cosmetic is 3% to 5%.

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