A poultry frozen semen sample detection device and use method thereof

Abstract

The present invention discloses a poultry frozen semen sample detection device and a method for using the same, and relates to the field of sample detection devices, including a support frame, a top plate provided with a transposition unit for rotating a trypan blue sampling tube and a duck semen sampling tube, and a collecting hopper provided with a rotating member. The present invention provides a rotating member, and when the trypan blue sampling tube rotates relative to the top plate, the disc is driven to rotate, so that the first contact piece is in contact with the second contact piece, and at this time, the second motor is powered on and operated, and the operation of the second motor causes the second spur gear to drive the first spur gear to rotate, so that the collecting hopper is operated, so that the duck semen discharged from the duck semen sampling tube and the trypan blue solution discharged from the trypan blue sampling tube alternately fall into the collecting hopper, so that the duck semen is fully in contact with the trypan blue solution, so as to reduce the reaction time of the duck semen and the trypan blue solution falling into the collecting hopper, and improve the overall detection efficiency of the equipment.

Description

Technical Field

[0001] The invention relates to the field of sample detection devices, in particular to a poultry frozen semen sample detection device and a use method thereof. Background Art

[0002] Poultry semen cryopreservation is a practical and effective method for long-term preservation of avian genetic resources. However, due to the particularity of avian sperm, although poultry semen cryopreservation has been widely studied since the 1940s, the current cryopreservation technology can be used to place sperm, eggs, embryos, etc. in an environment of -196°C to inhibit their metabolism, thereby preserving them. They can be thawed and used when needed. After thawing, their survival rate needs to be tested. Generally, sperm motility is identified by dropping trypan blue into duck semen samples. The basic principle is that when the cell membrane structure is intact, trypan blue can be repelled and prevented from entering the cell; while sperm cells that have lost their activity or have incomplete cell membranes have increased cell membrane permeability and can be stained blue by trypan blue. The survival rate is then calculated based on the proportion of blue cells.

[0003] Generally, when performing a survival rate test on thawed duck semen, it is necessary to first take a sample from the duck semen sample, inject the taken sample into a reagent tube, then extract a certain amount of trypan blue, inject the extracted trypan blue into the reagent tube containing duck semen, let it stand for a period of time, and then extract the mixture of duck semen and trypan blue for testing. This process is cumbersome, which not only affects the detection efficiency, but also easily causes excessive dripping when the mixture of duck semen and trypan blue is dripped on the slide, affecting the detection effect. Summary of the invention

[0004] The purpose of the present invention is to provide a poultry frozen semen sample detection device and a use method thereof in order to solve the problem of low detection efficiency.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a poultry frozen semen sample detection device, comprising a support frame, a positioning plate is installed on the top of the support frame, a first positioning column is arranged on the inner side of the support frame and is located below the positioning plate, a placement plate is installed on the top of the first positioning column, an observation platform is arranged on the side of the placement plate away from the support frame, a mounting frame is installed on the top of the observation platform, a microscope is installed on the top of the mounting frame, arc clamps are clamped at both ends of the positioning plate through torsion springs, a first motor is installed on the top of the support frame, a reciprocating screw is connected to the output end of the first motor, and a first A set of blocks, a top plate located above the positioning plate is installed on one side of the first set of blocks, a second set of blocks is arranged on the side of the top plate away from the first set of blocks, a guide column penetrating the second set of blocks is installed on the top of the support frame, one end of the top plate is rotatably connected to a duck semen sampling tube through a rotating shaft, and the end of the top plate away from the duck semen sampling tube is rotatably connected to a trypan blue sampling tube through a rotating shaft, a movable extraction part connected to the trypan blue sampling tube and the duck semen sampling tube is arranged on the top of the top plate, a transposition unit for rotating the trypan blue sampling tube and the duck semen sampling tube is arranged on the top plate, a collecting hopper located below the top plate is installed on the top of the positioning plate, and a rotating part is arranged on the collecting hopper.

[0006] As a further scheme of the present invention: the movable extraction part includes a first placement rack installed on the top of the top plate, a second placement rack is installed on the top of the first placement rack, a telescopic cylinder is installed on the top of the second placement rack, the output end of the telescopic cylinder is connected to a second corrugated telescopic tube connected to the top of the first placement rack and located on the inner side of the second placement rack, the bottom end of the second corrugated telescopic tube is connected to a connecting hose, the inner side of the duck semen sampling tube is plugged with a second piston rod extending to the top of the duck semen sampling tube, the inner side of the trypan blue sampling tube is plugged with a first piston rod extending to the top of the trypan blue sampling tube, a pull-up plate connected to the connecting hose is installed on the top of the second piston rod, the bottom of the pull-up plate is provided with a first corrugated telescopic tube connected to one side of the top of the duck semen sampling tube, and the bottom of the pull-up plate is provided with a reset spring located on the outside of the first corrugated telescopic tube and connected to one side of the top of the duck semen sampling tube.

[0007] As a further solution of the present invention: the first piston rod and the trypan blue sampling tube are also connected through a pull plate, a return spring and a first corrugated telescopic tube.

[0008] As a further scheme of the present invention: the transposition unit includes a single spiral screw which is rotatably connected to the top of the top plate through a bearing and is located on the inner side of the first placement rack, the top of the top plate is provided with a second positioning column arranged side by side with the single spiral screw, the outer side of the single spiral screw is sleeved with a movable plate which is slidably connected to the second positioning column, the movable plate is provided with a rack plate located on the inner side of the first set block, one side of the top plate is rotatably connected to a rotating connecting shaft through a bearing, second transmission bevel gears are installed at both ends of the rotating connecting shaft, a steering gear meshing with the rack plate is provided on the rotating connecting shaft, the duck semen sampling tube is connected to the top plate at one end of the rotating shaft at which the first transmission bevel gear is meshed with the second transmission bevel gear, and the trypan blue sampling tube is connected to the top plate at one end of the rotating shaft at which the first transmission bevel gear is also provided.

[0009] As a further solution of the present invention: the second transmission bevel gears at both ends of the rotating connection shaft are symmetrically arranged along the vertical center axis of the rotating connection shaft.

[0010] As a further solution of the present invention: the movable plate is provided with a threaded hole matching the outer thread of the single-screw screw.

[0011] As a further solution of the present invention: the rotating part includes a disc installed on the end of the first transmission bevel gear away from the trypan blue sampling tube, the disc is provided with an offset pin, the outer side of the offset pin is sleeved with a straight tooth groove, one side of the positioning plate is provided with a connecting bin which is arranged horizontally side by side with the positioning plate and located below the straight tooth groove, the bottom of the straight tooth groove is provided with an extension plate extending to the interior of the connecting bin, the top of the inner wall of the connecting bin is provided with a second contact piece, the bottom end of the extension plate is provided with a first contact piece located below the second contact piece, the outer side of the collecting hopper is provided with a first spur gear located above the positioning plate, the bottom of the positioning plate is provided with a second motor located on one side of the collecting hopper, and the output end of the second motor is connected to a second spur gear meshing with the first spur gear.

[0012] As a further solution of the present invention: the offset pin and the center of the disc are in a misaligned state, and the width of the inner part of the straight tooth groove is equal to the diameter of the offset pin.

[0013] As a further solution of the present invention: the second contact piece is electrically connected to the second motor through a wire, and the first contact piece is electrically connected to an external power supply through a wire.

[0014] The present invention also discloses a poultry frozen semen sample detection method, which uses the poultry frozen semen sample detection device and comprises the following steps:

[0015] S1: first open the arc clamp, place the test tube containing the thawed duck semen under the duck semen sampling tube, then loosen the arc clamp, limit the test tube by the arc clamp, and similarly place the test tube containing the trypan blue solution under the trypan blue sampling tube;

[0016] S2: placing a glass slide on the top of the placement plate so that it is located below the collecting hopper, starting the first motor, and driving the reciprocating screw to rotate by the first motor so that the top plate limited by the guide column and the second set of blocks moves downward, so that the duck semen sampling tube is inserted into the test tube containing the thawed duck semen, and the trypan blue sampling tube is inserted into the test tube containing the trypan blue solution. When the top plate moves to the lowest point and moves upward along the reciprocating screw, the movable extraction part is started, so that the duck semen sampling tube and the trypan blue sampling tube extract the duck semen and the trypan blue solution during the upward movement, so as to ensure the extraction of different liquid levels, thereby improving the detection range;

[0017] S3: When the top plate moves to the highest point, the first motor is turned off, and then the bottom ends of the duck semen sampling tube and the trypan blue sampling tube are swung to the top of the collecting hopper by operating the transposition unit. During this process, the rotating part operates to rotate the collecting hopper relative to the positioning plate. At this time, the duck semen sampling tube and the trypan blue sampling tube are operated to make the duck semen and the trypan blue solution inside fall into the collecting hopper. With the rotation of the collecting hopper, the duck semen and the trypan blue solution are alternately rotated and fall into the collecting hopper, so that the duck semen and the trypan blue solution are fully in contact;

[0018] S4: After the mixture of duck semen and trypan blue solution in the collecting hopper is placed for a period of time, the bottom of the collecting hopper is opened to allow the mixture in the collecting hopper to fall onto the slide on the top of the placement plate, and then the slide with the mixture of duck semen and trypan blue solution is placed on the observation table;

[0019] S5: The thawed duck semen is observed under a microscope, and the survival rate in the duck semen is calculated based on the color of the cells in the duck semen.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. By setting a movable extraction part, the telescopic cylinder is extended to compress the second bellows telescopic tube. At this time, the air inside the second bellows telescopic tube will enter the first bellows telescopic tube through the connecting hose, so that the second piston rod is moved upward relative to the duck semen sampling tube. Similarly, the first piston rod is moved upward relative to the trypan blue sampling tube. In this way, the duck semen and the trypan blue solution can be drawn into the duck semen sampling tube and the trypan blue sampling tube respectively, so that the amount of the duck semen and the trypan blue solution are equal. Then, the duck semen and the trypan blue solution inside the duck semen sampling tube and the trypan blue sampling tube are made to fall into the collecting hopper by extending and retracting the telescopic cylinder, so as to achieve a one-to-one mixing of the duck semen and the trypan blue solution, thereby improving the accuracy of subsequent detection data;

[0022] 2. By setting a transposition unit, the moving plate limited by the second positioning column is moved up along the single spiral screw by rotating the single spiral screw, so that the rack plate can turn the steering gear to rotate as the moving plate moves up, so that the rotating connecting shaft drives the duck semen sampling tube and the trypan blue sampling tube to swing towards each other through the second transmission bevel gear and the first transmission bevel gear, so that the bottom ends of the duck semen sampling tube and the trypan blue sampling tube are swung toward the vertical center axis of the top plate, so that the duck semen sampling tube and the trypan blue sampling tube are moved to the top of the collecting hopper. In this process, the duck semen sampling tube and the trypan blue sampling tube have a small range of movement, thereby preventing the duck semen and the trypan blue solution inside the duck semen sampling tube and the trypan blue sampling tube from falling onto the workbench, so as to ensure a clean and tidy working environment;

[0023] 3. By setting a rotating part, when the trypan blue sampling tube rotates relative to the top plate, the disc will be driven to rotate. At this time, the disc drives the extension plate to move upward through the offset pin and the straight tooth groove, so that the first contact piece is in contact with the second contact piece. At this time, the second motor is energized and operated. The operation of the second motor drives the first straight gear to rotate, so that the collecting hopper is operated, so that the duck semen discharged from the duck semen sampling tube and the trypan blue solution discharged from the trypan blue sampling tube fall into the collecting hopper alternately, so that the duck semen and the trypan blue solution are fully in contact, thereby reducing the reaction time of the duck semen and the trypan blue solution falling into the collecting hopper, and improving the overall detection efficiency of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a structural schematic diagram of the present invention;

[0025] Figure 2 It is a schematic diagram of the connection between the clamping plate and the supporting frame of the present invention;

[0026] Figure 3 It is a structural schematic diagram of the clamping plate of the present invention;

[0027] Figure 4It is a schematic diagram of the top structure of the top plate of the present invention;

[0028] Figure 5 It is a schematic diagram of the connection between the duck semen sampling tube and the trypan blue sampling tube of the present invention;

[0029] Figure 6 It is a structural schematic diagram of the movable extraction member of the present invention;

[0030] Figure 7 It is a structural schematic diagram of the rotating member of the present invention;

[0031] Figure 8 It is a schematic diagram of the internal structure of the connection warehouse of the present invention.

[0032] In the figure: 1, support frame; 2, first positioning column; 3, placement plate; 4, observation platform; 5, mounting frame; 6, microscope; 701, positioning plate; 702, arc clamp; 703, duck semen sampling tube; 704, guide column; 705, top plate; 706, first placement rack; 707, second placement rack; 708, telescopic cylinder; 709, trypan blue sampling tube; 710, extension plate; 711, reciprocating screw rod; 712, connecting bin; 713, first motor; 714, collecting hopper; 715, first spur gear; 716, second spur gear; 717, second motor; 718, first Set block; 719, second set block; 720, first piston rod; 721, connecting hose; 722, pull plate; 723, return spring; 724, first bellows telescopic tube; 725, second piston rod; 726, second bellows telescopic tube; 727, first transmission bevel gear; 728, second transmission bevel gear; 729, steering gear; 730, rack plate; 731, disc; 732, second positioning column; 733, single spiral screw; 734, moving plate; 735, rotating connecting shaft; 736, straight tooth groove; 737, offset pin; 738, first contact piece; 739, second contact piece. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance. In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", and "set" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, or it can be the internal connection of two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. The following is an explanation of an embodiment of the present invention based on the overall structure of the present invention.

[0035] See also Figures 1 to 8 In an embodiment of the present invention, a poultry frozen semen sample detection device includes a support frame 1, a positioning plate 701 is installed on the top of the support frame 1, a first positioning column 2 is arranged on the inner side of the support frame 1 and is located below the positioning plate 701, a placement plate 3 is installed on the top of the first positioning column 2, an observation platform 4 is arranged on the side of the placement plate 3 away from the support frame 1, a mounting frame 5 is installed on the top of the observation platform 4, a microscope 6 is installed on the top of the mounting frame 5, arc clamps 702 are clamped at both ends of the positioning plate 701 through torsion springs, a first motor 713 is installed on the top of the support frame 1, a reciprocating screw rod 711 is connected to the output end of the first motor 713, a first set block 718 is sleeved on the outer side of the reciprocating screw rod 711, and a first set block 718 is installed on one side of the first set block 718. A top plate 705 above 701, a second set of blocks 719 is arranged on the side of the top plate 705 away from the first set of blocks 718, a guide column 704 penetrating the second set of blocks 719 is installed on the top of the support frame 1, one end of the top plate 705 is rotatably connected to a duck semen sampling tube 703 through a rotating shaft, and one end of the top plate 705 away from the duck semen sampling tube 703 is rotatably connected to a trypan blue sampling tube 709 through a rotating shaft, a movable extraction part connected to the trypan blue sampling tube 709 and the duck semen sampling tube 703 is arranged on the top of the top plate 705, a transposition unit for rotating the trypan blue sampling tube 709 and the duck semen sampling tube 703 is arranged on the top plate 705, and a collecting hopper 714 located below the top plate 705 is installed on the top of the positioning plate 701, and a rotating part is arranged on the collecting hopper 714.

[0036] In this embodiment, the arc clamp 702 is first opened, and the test tube containing the thawed duck semen is placed under the duck semen sampling tube 703, and then the arc clamp 702 is loosened, and the test tube is limited by the arc clamp 702. Similarly, the test tube containing the trypan blue solution is placed under the trypan blue sampling tube 709, and then the slide is placed on the top of the placement plate 3 so that it is located under the collecting hopper 714, and the first motor 713 is started. The reciprocating screw rod 711 is driven by the first motor 713 to rotate so that the guide column 704 and the second set The top plate 705 limited by the block 719 moves downward, so that the duck semen sampling tube 703 is inserted into the test tube containing the thawed duck semen, and the trypan blue sampling tube 709 is inserted into the test tube containing the trypan blue solution. When the top plate 705 moves to the lowest point and moves upward along the reciprocating screw rod 711, the movable extraction member is started, so that the duck semen sampling tube 703 and the trypan blue sampling tube 709 extract the duck semen and the trypan blue solution during the upward movement, so as to ensure the extraction of different liquid levels, so as to improve the detection range. When the top plate 705 moves upward, the duck semen sampling tube 703 and the trypan blue sampling tube 709 extract the duck semen and the trypan blue solution. After reaching the highest point, the first motor 713 is turned off, and then the bottom ends of the duck semen sampling tube 703 and the trypan blue sampling tube 709 are swung to the top of the collecting hopper 714 by operating the transposition unit. During this process, the rotating part operates to rotate the collecting hopper 714 relative to the positioning plate 701. At this time, the duck semen sampling tube 703 and the trypan blue sampling tube 709 are operated to make the duck semen and the trypan blue solution fall into the collecting hopper 714, and the duck semen and the trypan blue solution are exchanged with each other in coordination with the rotation of the collecting hopper 714. The duck semen is rotated and falls into the collecting hopper 714, so that the duck semen and the trypan blue solution are fully in contact. After that, the mixed liquid of the duck semen and the trypan blue solution in the collecting hopper 714 is placed for a period of time, and then the bottom of the collecting hopper 714 is opened to allow the mixed liquid in the collecting hopper 714 to fall onto the slide on the top of the placement plate 3. Then, the slide with the mixed liquid of the duck semen and the trypan blue solution is placed on the observation table 4, and the thawed duck semen is observed through a microscope 6, and the survival rate in the duck semen is calculated according to the color of the cells in the duck semen.

[0037] Please refer to Figure 1 , Figure 4 , Figure 5 , Figure 6The movable extraction member includes a first placement rack 706 installed on the top of the top plate 705, a second placement rack 707 is installed on the top of the first placement rack 706, a telescopic cylinder 708 is installed on the top of the second placement rack 707, the output end of the telescopic cylinder 708 is connected to a second corrugated telescopic tube 726 connected to the top of the first placement rack 706 and located on the inner side of the second placement rack 707, the bottom end of the second corrugated telescopic tube 726 is connected to a connecting hose 721, and the inner side of the duck semen sampling tube 703 is plugged with a second bellows extending to the duck semen sampling tube 707. The second piston rod 725 is provided at the top of the duck semen sampling tube 703, the first piston rod 720 extending to the top of the trypan blue sampling tube 709 is inserted into the inner side of the trypan blue sampling tube 709, a pull plate 722 connected to the connecting hose 721 is installed on the top of the second piston rod 725, a first corrugated telescopic tube 724 connected to one side of the top of the duck semen sampling tube 703 is provided at the bottom of the pull plate 722, and a return spring 723 located outside the first corrugated telescopic tube 724 and connected to one side of the top of the duck semen sampling tube 703 is provided at the bottom of the pull plate 722.

[0038] In this embodiment, after the duck semen sampling tube 703 and the trypan blue sampling tube 709 are respectively inserted into the duck semen and the trypan blue solution, the telescopic cylinder 708 is started to extend the telescopic cylinder 708. At this time, the output end of the telescopic cylinder 708 pushes the top of the second bellows telescopic tube 726 to compress the second bellows telescopic tube 726. At this time, the air inside the second bellows telescopic tube 726 enters the first bellows telescopic tube 724 through the connecting hose 721, so that the second piston rod 725 is relatively moved relative to the duck semen sampling tube 703. Similarly, the first piston rod 720 moves upward relative to the trypan blue sampling tube 709, so that the duck semen and the trypan blue solution can be drawn into the duck semen sampling tube 703 and the trypan blue sampling tube 709 respectively, so as to make the amount of duck semen and the trypan blue solution equal, and then the telescopic cylinder 708 is extended and retracted to make the duck semen and the trypan blue solution inside the duck semen sampling tube 703 and the trypan blue sampling tube 709 fall into the collecting hopper 714, thereby achieving a one-to-one mixing of the duck semen and the trypan blue solution, thereby improving the accuracy of subsequent detection data.

[0039] Please refer to Figure 6 The first piston rod 720 and the trypan blue sampling tube 709 are also connected through the pull plate 722, the return spring 723, and the first corrugated telescopic tube 724.

[0040] In the present embodiment: this structure is provided to make the amount of trypan blue solution extracted by the first piston rod 720 moving upward relative to the trypan blue sampling tube 709 equal to the amount of duck semen extracted by the second piston rod 725 moving upward relative to the duck semen sampling tube 703, thereby achieving a one-to-one mixing of the subsequent duck semen and the trypan blue solution, preventing the amount of trypan blue solution from being too much relative to the amount of duck semen and causing a waste of the trypan blue solution, and at the same time preventing the amount of trypan blue solution from being too little relative to the amount of duck semen and affecting the accuracy of the detection data.

[0041] Please refer to Figure 1 , Figure 4 , Figure 5 The transposition unit includes a single screw rod 733 which is rotatably connected to the top of the top plate 705 through a bearing and is located on the inner side of the first placement frame 706. A second positioning column 732 which is arranged side by side with the single screw rod 733 is arranged on the top of the top plate 705. A moving plate 734 which is slidably connected to the second positioning column 732 is sleeved on the outer side of the single screw rod 733. A rack plate 730 which is located on the inner side of the first set block 718 is arranged on the moving plate 734. One side of the top plate 705 is rotatably connected to the top plate 705 through a bearing. It is dynamically connected with a rotating connecting shaft 735, and second transmission bevel gears 728 are installed at both ends of the rotating connecting shaft 735. A steering gear 729 meshing with the rack plate 730 is provided on the rotating connecting shaft 735. The end of the rotating shaft connecting the duck semen sampling tube 703 and the top plate 705 is provided with a first transmission bevel gear 727 meshing with the second transmission bevel gear 728, and the end of the rotating shaft connecting the trypan blue sampling tube 709 and the top plate 705 is also provided with a first transmission bevel gear 727.

[0042] In this embodiment, the single screw rod 733 is rotated, and the movable plate 734 limited by the second positioning column 732 is moved up along the single screw rod 733 by the rotation of the single screw rod 733, so that the rack plate 730 can turn the steering gear 729 to rotate as the movable plate 734 moves up, so that the rotating connecting shaft 735 drives the duck semen sampling tube 703 and the trypan blue sampling tube 709 to rotate correspondingly through the second transmission bevel gear 728 and the first transmission bevel gear 727. The duck semen sampling tube 703 and the trypan blue sampling tube 709 are swung toward the vertical center axis of the top plate 705, so that the duck semen sampling tube 703 and the trypan blue sampling tube 709 are moved to the top of the collecting hopper 714. During this process, the duck semen sampling tube 703 and the trypan blue sampling tube 709 have a smaller range of movement, thereby preventing the duck semen sampling tube 703 and the trypan blue solution inside the duck semen sampling tube 703 and the trypan blue sampling tube 709 from falling onto the workbench, thereby ensuring a clean and tidy working environment.

[0043] Please refer to Figure 5 The second transmission bevel gears 728 at both ends of the rotating connecting shaft 735 are symmetrically arranged along the vertical center axis of the rotating connecting shaft 735.

[0044] In this embodiment: by setting this structure, when the rotating connecting shaft 735 rotates, the second transmission bevel gear 728 and the first transmission bevel gear 727 make the trypan blue sampling tube 709 and the duck semen sampling tube 703 rotate in opposite directions, so that the bottom ends of the trypan blue sampling tube 709 and the duck semen sampling tube 703 swing towards each other.

[0045] Please refer to Figure 5 The movable plate 734 is provided with a threaded hole matching the outer thread of the single screw rod 733 .

[0046] In this embodiment: by setting this structure, when the single screw rod 733 rotates, the moving plate 734 moves upward along the single screw rod 733, thereby improving the stability of the rack plate 730 driving the steering gear 729 to rotate.

[0047] Please refer to Figure 1 , Figure 3 , Figure 7 , Figure 8 The rotating member includes a disc 731 installed on the end of the first transmission bevel gear 727 away from the trypan blue sampling tube 709, a biasing pin 737 is provided on the disc 731, and a straight tooth groove 736 is sleeved on the outer side of the biasing pin 737. A connecting bin 712 which is arranged horizontally side by side with the positioning plate 701 and located below the straight tooth groove 736 is installed on one side of the positioning plate 701, an extension plate 710 extending to the inside of the connecting bin 712 is installed at the bottom of the straight tooth groove 736, a second contact piece 739 is installed on the top of the inner wall of the connecting bin 712, and a first contact piece 738 located below the second contact piece 739 is installed at the bottom of the extension plate 710. A first spur gear 715 located above the positioning plate 701 is provided on the outer side of the collecting hopper 714, and a second motor 717 located on one side of the collecting hopper 714 is provided at the bottom of the positioning plate 701. The output end of the second motor 717 is connected to a second spur gear 716 meshing with the first spur gear 715.

[0048] In this embodiment: when the trypan blue sampling tube 709 rotates relative to the top plate 705, it will drive the disc 731 to rotate. At this time, the disc 731 drives the extension plate 710 to move upward through the offset pin 737 and the straight tooth groove 736, so that the first contact piece 738 is in contact with the second contact piece 739. At this time, the second motor 717 is powered on and operated. The operation of the second motor 717 drives the first straight gear 715 to rotate, so that the collecting hopper 714 is operated, so that the duck semen discharged from the duck semen sampling tube 703 is fully in contact with the trypan blue solution discharged from the trypan blue sampling tube 709, so as to reduce the reaction time of the duck semen falling into the collecting hopper 714 and the trypan blue solution, thereby improving the overall detection efficiency of the equipment.

[0049] Please refer to Figure 7 The offset pin 737 and the center of the disk 731 are in a misaligned state, and the width inside the straight tooth groove 736 is equal to the diameter of the offset pin 737.

[0050] In this embodiment: by setting this structure, when the offset pin 737 rotates along the center of the disk 731, the straight tooth groove 736 drives the extension plate 710 limited by the connecting bin 712 to move, thereby improving the accuracy of the docking between the first contact piece 738 and the second contact piece 739.

[0051] Please refer to Figure 7 , Figure 8 The second contact piece 739 is electrically connected to the second motor 717 through a wire, and the first contact piece 738 is electrically connected to the external power supply through a wire.

[0052] In this embodiment, by providing this structure, the second motor 717 is operated when the second contact piece 739 contacts the first contact piece 738 , thereby controlling the operation timing of the second motor 717 .

[0053] In combination with the above-mentioned poultry frozen semen sample detection device, a poultry frozen semen sample detection method is provided below, which specifically includes the following steps:

[0054] S1: weigh 0.5-0.75 g D-fructose, 0.6-0.9 g sodium glutamate, 0.034-0.068 g magnesium chloride hexahydrate, 0.2-0.43 g sodium acetate, 0.06-0.08 g sodium citrate, 0.05-0.065 g potassium dihydrogen phosphate, 0.3-0.5 g disodium hydrogen phosphate, 0.15-0.2 g N-trimethyl-2-aminoethanesulfonic acid, 0.1 g penicillin, 0.1 g streptomycin and double distilled water;

[0055] S2: Dissolve the raw materials weighed in step 1 in 100 ml of double distilled water, adjust the pH value to 7.0-7.4, and prepare duck sperm diluent:

[0056] S3: artificially inducing the collection of duck semen, taking a small amount of the duck sperm diluent prepared in step 2 to dilute the duck semen, observing sperm motility under a microscope and counting the sperm;

[0057] S4: dilute the duck semen with duck sperm diluent at a ratio of 1:3 according to the counting results;

[0058] S5: adding 12-20% of cryoprotectant DMA to the duck sperm diluent according to the volume ratio, and then freezing the duck semen mixed with the cryoprotectant, and taking out the duck semen frozen sample after freezing for a period of time;

[0059] S6: Open the arc clamp 702, place the test tube containing the thawed duck semen under the duck semen sampling tube 703, then loosen the arc clamp 702, limit the test tube by the arc clamp 702, and similarly place the test tube containing the trypan blue solution under the trypan blue sampling tube 709;

[0060] S7: Place the glass slide on the top of the placement plate 3 so that it is located below the collecting hopper 714, start the first motor 713, and drive the reciprocating screw 711 to rotate through the first motor 713 so that the top plate 705 limited by the guide column 704 and the second set of blocks 719 moves downward, so that the duck semen sampling tube 703 is inserted into the test tube containing the thawed duck semen, and the trypan blue sampling tube 709 is inserted into the test tube containing the trypan blue solution. When the top plate 705 moves to the lowest point and moves upward along the reciprocating screw 711, start the telescopic cylinder 708 to extend the telescopic cylinder 708. The output end of the cylinder 708 will push the top of the second bellows expansion tube 726, so that the second bellows expansion tube 726 is compressed. At this time, the air inside the second bellows expansion tube 726 will enter the first bellows expansion tube 724 through the connecting hose 721, so that the second piston rod 725 moves upward relative to the duck semen sampling tube 703. Similarly, the first piston rod 720 moves upward relative to the trypan blue sampling tube 709, so that the duck semen and the trypan blue solution can be drawn into the duck semen sampling tube 703 and the trypan blue sampling tube 709 respectively, so that the amount of duck semen and the trypan blue solution are equal;

[0061] S8: When the top plate 705 moves to the highest point, the first motor 713 is turned off, and the single screw rod 733 is rotated. The moving plate 734 limited by the second positioning column 732 is moved upward along the single screw rod 733 through the rotation of the single screw rod 733, so that the rack plate 730 can turn the steering gear 729 to rotate during the upward movement of the moving plate 734, so that the rotating connecting shaft 735 drives the duck semen sampling tube 703 and the trypan blue sampling tube 709 to swing toward each other through the second transmission bevel gear 728 and the first transmission bevel gear 727, so that the bottom ends of the duck semen sampling tube 703 and the trypan blue sampling tube 709 are swung toward the vertical center axis of the top plate 705, so that the duck semen sampling tube 703 and the trypan blue sampling tube 709 are moved to the top of the collecting hopper 714;

[0062] S9: The duck semen in the duck semen sampling tube 703 and the trypan blue solution in the trypan blue sampling tube 709 are made to fall into the collecting hopper 714 by extending the telescopic cylinder 708. At the same time, the disc 731 drives the extension plate 710 to move upward through the offset pin 737 and the straight tooth groove 736, so that the first contact piece 738 is in contact with the second contact piece 739. At this time, the second motor 717 is powered on and operated. The operation of the second motor 717 drives the second straight gear 716 to drive the first straight gear 715 to rotate, so that the collecting hopper 714 is operated, so that the duck semen discharged from the duck semen sampling tube 703 and the trypan blue solution discharged from the trypan blue sampling tube 709 are fully in contact, so as to reduce the reaction time of the duck semen and the trypan blue solution falling into the collecting hopper 714, and improve the overall detection efficiency of the equipment;

[0063] S10: After the mixed solution of duck semen and trypan blue solution in the collecting hopper 714 is placed for a period of time, the bottom of the collecting hopper 714 is opened, so that the mixed solution in the collecting hopper 714 falls onto the slide glass on the top of the placing plate 3, and then the slide glass with the mixed solution of duck semen and trypan blue solution is placed on the observation platform 4;

[0064] S11: observing the thawed duck semen through a microscope 6, calculating the survival rate in the duck semen according to the color of the cells in the duck semen, and then comparing the activity ratio of the duck semen before and after freezing.

[0065] What is described above is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A poultry frozen semen sample detection device, comprising a support frame (1), characterized in that: A positioning plate (701) is installed on the top of the support frame (1), a positioning column (2) located below the positioning plate (701) is arranged on the inner side of the support frame (1), a placement plate (3) is installed on the top of the positioning column (2), an observation platform (4) is arranged on the side of the placement plate (3) away from the support frame (1), a mounting frame (5) is installed on the top of the observation platform (4), a microscope (6) is installed on the top of the mounting frame (5), arc-shaped clamps (702) are clamped at both ends of the positioning plate (701) through torsion springs, a first motor (713) is installed on the top of the support frame (1), a reciprocating screw rod (711) is connected to the output end of the first motor (713), a first sleeve block (718) is sleeved on the outer side of the reciprocating screw rod (711), and a top plate (705) located above the positioning plate (701) is installed on one side of the first sleeve block (718). A second set of blocks (719) is provided on a side of the top plate (705) away from the first set of blocks (718); a guide column (704) penetrating the second set of blocks (719) is installed on the top of the support frame (1); one end of the top plate (705) is rotatably connected to a duck semen sampling tube (703) via a rotating shaft; and one end of the top plate (705) away from the duck semen sampling tube (703) is rotatably connected to a trypan blue sampling tube (709) via a rotating shaft. ), a movable extraction member connected to a trypan blue sampling tube (709) and a duck semen sampling tube (703) is disposed on the top of the top plate (705), a transposition unit for rotating the trypan blue sampling tube (709) and the duck semen sampling tube (703) is disposed on the top plate (705), a collecting hopper (714) located below the top plate (705) is installed on the top of the positioning plate (701), and a rotating member is disposed on the collecting hopper (714); The movable extraction member comprises a first placement rack (706) installed on the top of the top plate (705), a second placement rack (707) is installed on the top of the first placement rack (706), a telescopic cylinder (708) is installed on the top of the second placement rack (707), the output end of the telescopic cylinder (708) is connected to a second corrugated telescopic tube (726) connected to the top of the first placement rack (706) and located on the inner side of the second placement rack (707), the bottom end of the second corrugated telescopic tube (726) is connected to a connecting hose (721), and the inner side of the duck semen sampling tube (703) is plugged with a second bellows extending to the duck semen sampling tube (703). 03) at the top, the first piston rod (720) extending to the top of the trypan blue sampling tube (709) is inserted into the inner side of the trypan blue sampling tube (709), a pull plate (722) connected to the connecting hose (721) is installed on the top of the second piston rod (725), the bottom of the pull plate (722) is provided with a first corrugated telescopic tube (724) connected to one side of the top of the duck semen sampling tube (703), and the bottom of the pull plate (722) is provided with a return spring (723) located outside the first corrugated telescopic tube (724) and connected to one side of the top of the duck semen sampling tube (703); The transposition unit comprises a single spiral screw (733) which is rotatably connected to the top of the top plate (705) through a bearing and is located on the inner side of the first placement frame (706); a positioning column (732) which is arranged side by side with the single spiral screw (733) is arranged on the top of the top plate (705); a moving plate (734) which is slidably connected to the positioning column (732) is sleeved on the outer side of the single spiral screw (733); a rack plate (730) which is located on the inner side of the first set block (718) is arranged on the moving plate (734); one side of the top plate (705) is rotatably connected to the top plate (705) through a bearing; The rotating shaft (735) is rotatably connected to the top plate (705), and the two ends of the rotating shaft (735) are equipped with second transmission bevel gears (728). The rotating shaft (735) is provided with a steering gear (729) meshing with the rack plate (730). The end of the rotating shaft connecting the duck semen sampling tube (703) and the top plate (705) is provided with a first transmission bevel gear (727) meshing with the second transmission bevel gear (728). The end of the rotating shaft connecting the trypan blue sampling tube (709) and the top plate (705) is also provided with a first transmission bevel gear (727). The rotating member comprises a disc (731) mounted on one end of the first transmission bevel gear (727) away from the trypan blue sampling tube (709), a deflection pin (737) is arranged on the disc (731), and a straight tooth groove (736) is sleeved on the outer side of the deflection pin (737), a connecting bin (712) is installed on one side of the positioning plate (701) and is arranged in parallel with the positioning plate (701) and is located below the straight tooth groove (736), and an extension plate (710) extending to the inside of the connecting bin (712) is installed at the bottom of the straight tooth groove (736), and the A second contact piece (739) is installed at the top of the inner wall of the connecting bin (712); a first contact piece (738) located below the second contact piece (739) is installed at the bottom end of the extension plate (710); a first spur gear (715) located above the positioning plate (701) is arranged on the outer side of the collecting hopper (714); a second motor (717) located on one side of the collecting hopper (714) is arranged at the bottom of the positioning plate (701); an output end of the second motor (717) is connected to a second spur gear (716) meshing with the first spur gear (715).

2. A poultry frozen semen sample detection device according to claim 1, characterized in that: The first piston rod (720) and the trypan blue sampling tube (709) are also connected via a pull plate (722), a return spring (723), and a first corrugated telescopic tube (724).

3. A poultry frozen semen sample detection device according to claim 1, characterized in that: The second transmission bevel gears (728) at both ends of the rotating connection shaft (735) are symmetrically arranged along the vertical center axis of the rotating connection shaft (735).

4. A poultry frozen semen sample detection device according to claim 1, characterized in that: The movable plate (734) is provided with a threaded hole matching the outer thread of the single screw rod (733).

5. The poultry frozen semen sample detection device according to claim 1, characterized in that: The offset pin (737) and the center of the disc (731) are in a misaligned state, and the width inside the straight tooth groove (736) is equal to the diameter of the offset pin (737).

6. A poultry frozen semen sample detection device according to claim 1, characterized in that: The second contact piece (739) is electrically connected to the second motor (717) via a wire, and the first contact piece (738) is electrically connected to an external power source via a wire.

7. A method for detecting poultry frozen semen samples, characterized in that: A poultry frozen semen sample detection device according to any one of claims 1 to 6 is used, comprising the following steps: S1: first open the arc clamp (702), place the test tube containing the thawed duck semen under the duck semen sampling tube (703), then loosen the arc clamp (702), limit the test tube by the arc clamp (702), and similarly place the test tube containing the trypan blue solution under the trypan blue sampling tube (709); S2: placing a glass slide on the top of the placement plate (3) so that it is located below the collecting hopper (714), starting the first motor (713), and driving the reciprocating screw (711) to rotate through the first motor (713) so that the top plate (705) limited by the guide column (704) and the second set of blocks (719) moves downward, so that the duck semen sampling tube (703) is inserted into the test tube containing the thawed duck semen, and the trypan blue sampling tube (709) is inserted into the test tube containing the trypan blue solution. When the top plate (705) moves to the lowest point and moves upward along the reciprocating screw (711), the movable extraction member is started, so that the duck semen sampling tube (703) and the trypan blue sampling tube (709) extract the duck semen and the trypan blue solution during the upward movement, so as to ensure the extraction of different liquid levels, thereby improving the detection range; S3: When the top plate (705) moves to the highest point, the first motor (713) is turned off, and then the bottom ends of the duck semen sampling tube (703) and the trypan blue sampling tube (709) are swung to the top of the collecting hopper (714) by operating the transposition unit. During this process, the rotating member operates to rotate the collecting hopper (714) relative to the positioning plate (701). At this time, the duck semen and trypan blue solution in the duck semen sampling tube (703) and the trypan blue sampling tube (709) fall into the collecting hopper (714) through the operation of the movable extraction member. With the rotation of the collecting hopper (714), the duck semen and the trypan blue solution rotate alternately and fall into the collecting hopper (714), so that the duck semen and the trypan blue solution are fully in contact; S4: After the mixture of duck semen and trypan blue solution in the collecting hopper (714) is placed for a period of time, the bottom of the collecting hopper (714) is opened, so that the mixture in the collecting hopper (714) falls onto the slide on the top of the placement plate (3), and then the slide with the mixture of duck semen and trypan blue solution is placed on the observation platform (4); S5: Observe the thawed duck semen through a microscope (6), and calculate the survival rate in the duck semen based on the color of the cells in the duck semen.

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